This is the result of a statistical analysis of the ...
E Value: Definition and Origin
This is the result of a statistical analysis of the similarity between the query sequence and the sequence in the table. A lower E value indicates a lower probability that the two sequences are aligned by chance alone. Originally this was presented as a “p value” (just like that used in a chi square test) but as the DNA database grew, the statistical method could not keep up. Soon there were so many sequences in the database that an alignment between two sequences might have a p value indicating that the alignment was biologically relevant (i.e., “real”), but in fact the two sequences were aligned due to chance. To avoid confusion over the usually accepted cut-off value for a p value, the score was changed to an E value. We now use a loose cut-off value of smaller than 10-6 (1e-06) to indicate sequences that are not merely aligned by chance.
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