ABSA International: The Association for Biosafety and ...
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Date: Mon, 4 Jan 1999 17:34:49 -0500
Reply-To: btepper@
Sender: A Biosafety Discussion List
From: "Byron S. Tepper"
Organization: @Home Network
Subject: Biosafety Course-Preliminary Announcement
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The Johns Hopkins University course - Control of Biohazards in the
Research Laboratory - will be presented on June 14-18,1999
Place: Omni Inner Harbor Hotel
101 West Fayette Street
Baltimore, MD 21201
Registration: 8:00-8:30 a.m., Monday, June 14, 1999
Fee: $1475 per person to include registration, continental breakfasts,
refreshments, 2 lunches and a banquet. All fees are payable in advance.
A letter confirming enrollment will be sent to each registrant. Foreign
payments must be made in a draft on a U.S. bank. Only one half of the
fee will be returned if withdrawal is made after May 14, 1999.
Course Credits: This program qualifies for 3 continuing education
credits from the Johns Hopkins University. This course also qualifies
for 4.5 ABIH Certification Maintenance Credits.
Course Manual: A course manual will provide lecture outlines, data
tables and graphics used in the lectures, and supporting materials.
Social Functions: A complimentary banquet for registrants will be held
on Thursday will be held on Thursday, June 17. Guests are invited for a
charge of $ 40.00.
Lodging: Accommodations have been reserved at the Omni Inner Harbor
Hotel. Rate: $159 Single or Double - guaranteed until May 14, 1999
For Further Information Contact
Dr. Byron S. Tepper
Phone: (410) 828-6330
Fax: (410) 828-6331
E-mail: btepper@
or
Dr. Richard W. Gilpin
Phone: (410) 955-5918
Fax: (410) 955-5929
E-mail: gilpin@welchlink.welch.jhu.edu
COURSE REGISTRATION AND HOTEL REGISTRATION FORMS FOLLOW:
Course Registration Form - Control of Biohazards in the Research
Laboratory - June 1999
Please mail remittance and completed registration to:
Becky Vaeth, Registrar
Center for Occupational and Environmental Health
The Johns Hopkins University
2024 E Monument Street, Suite 1-300
Baltimore, MD 21205-2223
Name:__________________________________________________________________
Address:________________________________________________________________
City: _____________________State: ____ Zip
Code:____________________________
Telephone Number: ( )_____________________ Fax ( ) ________________
Social Security Number: ________________________________________________
Employer________________________________________________________________
* Please make checks ($1,475 per person) payable to: JHU Biohazards
Course.
Check or purchase order must accompany registration form to guarantee
enrollment in the course.
Hotel Reservation Form Control of Biohazards in the Research Laboratory
- June 1999
Please complete and mail to:
Omni Inner Harbor Hotel
101 W. Fayette Street
Baltimore, MD 21201
Telephone: 410-752-1100 or (800) THE-OMNI
I am enrolled in the Biohazards Course presented by the Johns Hopkins
University, June 14 - 18, 1999. Please make the following reservation
for me.
Room Rates- $159 Single or Double, per night.
_____ Single(s) _____ Doubles for ______nights
Room rates guaranteed until May 14, 1998.
Name: __________________________________________________________________
Address:
________________________________________________________________
City State Zip
Code:_______________________________________________________
Area Code/ Telephone Number:
______________________________________________
Arrival Date _________________ Departure Date
_______________________________
Please hold my reservation for late arrival __________
Reservations may be guaranteed for late arrival by one night's deposit.
Include
check payable to the hotel or use major credit card.
AMEX________ VISA_________ MASTER CARD ________
Card #_____________________________________ Exp. Date___________________
Signature____________________________________
Date___________________
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Date: Wed, 6 Jan 1999 11:28:28 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Heather Gonsoulin
Subject: Summary re: respirator dliemma
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Thank you all for your answers to the question I posed regarding the =
respirator dilemma. I have compiled the responses and list them below.
1. Use PAPR with a clear hood: This will not suit our needs because if =
these babies are to be raised with as much human contact as possible, =
the hood creates a barrier.
2. Pump mother's milk to feed to the babies or bank it for later =
feeding: This may not be a feasible option because of the fact that the =
mothers would have to be sedated to obtain the milk. We try to sedate =
our animals only when absolutely necessary.
3. Vaccinate the chimp babies and mothers for the pneumonias they are =
susceptible to: This is most likely the path we will choose, along with =
a combination of the following suggestions.
4. Consistant personal hygiene: Shower before entering and after =
exiting the chimp nursery. Wash hands frequently and thoroughly. =20
5. Consistent chimp hygiene: Bathe the chimps daily. Make sure that =
sick chimps are not allowed to spread an illness to the rest of the =
group by isolating them until the illness is treated properly.
6. Create rules for the caretakers: For example: Do not go into chimp =
area if you have any of the following: cough, sore throat, fever, etc.
Again, I want to thank everyone for their input. The NET has done it's =
job!
Heather H. Gonsoulin
Occupational Health and Safety Officer
USL-NIRC
(318) 482-0306 (fax) 318-373-0057
hah8377@usl.edu
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Date: Wed, 6 Jan 1999 14:53:59 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Debra Hunt
Subject: Re: Summary re: respirator dliemma
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This is great!! Sounds just like we handle moms and babies of the human
variety in the hospital!!!!
Debra L. Hunt, DrPH, CBSP
Duke University Biological Safety
919-684-8822
e-mail: hunt0009@mc.duke.edu
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Date: Thu, 7 Jan 1999 15:04:02 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Mildew
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I am hoping that one of us has some experience with decontaminating film
and videotape and can help this person. I know how to do paper, but not
sure what to use on these materials. Thanks one and all -- respond to
Carlos directly (email address at end).
Richard Fink
Biosafty List Owner
rfink@mit.edu
>>>>
X-Comment: mitvma.mit.edu: Mail was sent by atlas.
From: "Carlos Gonzalez - "
To:
Subject:
Date: Thu, 7 Jan 1999 13:40:33 -0400
X-Msmail-Priority: Normal
X-Mailer: Microsoft Outlook Express 4.71.1712.3
X-Mimeole: Produced By Microsoft MimeOLE V4.71.1712.3
Our Company is in the Indoor Air Quality Industry and we have receive =
request from our customer to decontaminate (clean) mildew in audiovisual =
items such as Video Tapes, Micro-Film, Negatives, etc.. .
Please mail (or Fax) us information on equipments, techniques =
(procedures) and
products that can be use for this purpose. Any help will be appreciated
If you need any assistance here in Puerto Rico, please do not hesitate =
to contact us
Thanks (Gracias)
In-Viro Care Inc.
377 Escorial
San Juan, P.R. 00920-3508
Tel. 787 792-4190 Fax 787 792-3720
Site:
E:mail: carlos@
Contact: Carlos Gonzalez Boothby
on this subject and could supply me with links, animation, pictures, that
> revolve around this topic.
>
> I would like to make my presentations worthwhile - and fun!
>
> Thank you!
> Bliss M. Schlank
> Biosafety Specialist
> Zeneca Pharmaceuticals
> 1800 Concord Pike
> Wilmington DE 19064
> 302.886.2185
> Fax: 302.886.2909
> bliss.schlank@phwilm.
--------------BB91C53F8AB1EA0002D10766
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X-MIME-Autoconverted: from 8bit to quoted-printable by netstats.ouhsc.edu id RAA00668
NIOSH/Asthma in Animal Handlers Alert
&=
nbsp;
ALERT
Preventing Asthmain Animal Handlers
January 1998
DHHS (NIOSH) Publication No. 97-116
Disclaimer
Preventi=
ng Asthma in
Animal Handlers
WARNING!
Exposure to animals or animal products in the workplace can cause =
asthma
and allergies.
Animal handlers should take steps to protect themselves from exposure =
to animals and
animal products:=20
Perform animal manipulations within ventilated hoods or safety cabi=
nets when possible.
Avoid wearing street clothes while working with animals.
Leave work clothes at the workplace to avoid potential exposure pro=
blems for family
members.
Keep cages and animal areas clean.
Reduce skin contact with animal products such as dander, serum, and=
urine by using
gloves, lab coats, and approved particulate respirators with faceshie=
lds.
Employers of animal handlers should take steps to protect workers from=
exposure to
animals and animal products:
Modify ventilation and filtration systems:
=20
—Increase the ventilation rate and humidity in the animal-hou=
sing areas.
—Ventilate animal-housing and -handling areas separately from the re=
st of the facility.
—Direct airflow away from workers and toward the backs of the animal=
cages.
—Install ventilated animal cage racks or filter-top animal cages.
=20
Decrease animal density (number of animals per cubic meter of roo=
m volume).
Keep cages and animal areas clean.
Use absorbent pads for bedding. If these are not available, use c=
orncob bedding instead
of sawdust bedding.
Use an animal species or sex that is known to be less allergenic =
than others.
Provide protective equipment for animal handlersgloves, lab coats=
, and approved
particulate respirators with faceshields.
Provide training to educate workers about animal allergies and st=
eps for risk reduction.
Provide health monitoring and appropriate counseling and medical =
followup for workers
who have become sensitized or have developed allergy symptoms.
=
Preventing Asthma
in Animal Handlers
WARNING!
Exposure to animals or animal products in the workplace can cause =
asthma
and allergies.
The National Institute for Occupational Safety and Health (NIOSH) requ=
ests assistance
in preventing asthma and allergies in animal handlers. Approximately 2 mi=
llion workers
have jobs that require constant contact with animals or animal products [=
Brooks 1992].
About 33% of animal handlers have allergic symptoms, and approximately 10=
% have symptoms
of animal-induced asthma [Chan-Yeung and Malo 1994]. Animals or animal pr=
oducts such as
dander, hair, scales, fur, saliva, and body wastes contain powerful aller=
gens that can
cause both respiratory and skin disorders. Workers at risk include labora=
tory animal and
veterinary technicians, researchers, veterinarians, and others who have p=
rolonged, close
association with animals or their secretions or excretions. Also at risk =
are workers who
handle animal products or associated materials such as bedding and feed.
This Alert describes three case reports of workers affected by exposur=
es to animals.
The recommendations presented here can help reduce such exposures and pre=
vent
animal-induced asthma and allergies.
BACK=
GROUND
Animal-related asthma and allergies are exaggerated reactions of the b=
ody=3Ds immune system to anim=
al proteins, also known as
allergens. Sources of these allergens include animal dander, scales, fur,=
body wastes, and
saliva [Bardana 1992; Lincoln et al. 1974].
Inhalation is one of the most common ways for allergens to enter the b=
ody. After a
period of time (often several months, but occasionally many years), worke=
rs may inhale
sufficient quantities of allergens to become sensitizedCthat
is, they develop symptoms when exposed again, even to tiny amounts of the=
allergen
[Bardana 1992; Chan-Yeung and Malo 1994]. Airborne exposures to dusts der=
ived from animals
are not currently regulated to protect workers from developing allergic p=
roblems.
The diagnosis of animal allergy or sensitization is made using skin-pr=
ick tests, blood
antibody tests, and other methods. Symptoms vary among workers who have b=
ecome sensitized
to animals. Mild reactions include sneezing and runny nose. More serious =
reactions to an
inhaled allergen may result in asthma symptoms such as cough, chest tight=
ness, wheezing,
or shortness of breath. In sensitized workers, reactions often occur soon=
after exposure
to the animal or animal product, but they may be delayed for 2 to 8 hours=
or more.
A worker who has developed asthma symptoms from animal allergies often=
improves or
recovers completely if he or she immediately stops being exposed to dusts=
containing the
animal allergens. However, the longer the exposures continue, the more li=
kely the illness
will persist, even after all contact with animals has stopped.
Symptoms from animal-related asthma and allergies can be severe and ma=
y require
affected workers to change jobs or careers [Bardana 1992]. Affected worke=
rs and their
employers must bear the costs for treatment, time lost from work, and tem=
porary or even
permanent disability [Newill et al. 1986].
COMM=
ON
SOURCES OF EXPOSURE
Sources of exposure to animal allergens vary with animal species. For =
example, the most
important allergens have been found in the urine of rats and in the urine=
, saliva, and
pelts of guinea pigs [Chan-Yeung and Malo 1994]. Rat urine contains signi=
ficant amounts of
a protein that is also found in dust samples from ventilation systems of =
animal facilities
[Bardana 1992]. Other important sources of allergen exposure include rabb=
it pelts, cat
saliva and dander, dog dander, and horse serum and dander [Bardana 1992].=
Exposures to rats, mice, and rabbits have frequently been associated w=
ith the
development of occupational asthma. Species other than mammals have also =
been reported to
cause respiratory symptomsCva=
rious insects, for
example, and frogs (which are commonly used in science classes) [Bardana =
1992]. Exposures
to birds have been associated with other respiratory diseases, including =
hypersensitivity
pneumonitis [Parker et al. 1992]. A person who becomes allergic to one an=
imal species may
react to other species as well. Even a low exposure to these common sourc=
es of animal
allergens can result in allergies, but the risk increases as the worker=3Ds exposure increases [Hollan=
der et al. 1997].
TYPE=
S
OF ANIMAL HANDLERS AT RISK
All animal handlers appear to be at risk for developing work-related a=
llergy symptoms.
However, workers who had symptoms or signs of allergies before they were =
employed as
animal handlers are more likely to develop animal-induced asthma [Beckett=
1994; Chan-Yeung
and Malo 1994]. Allergic workers, particularly those sensitized to domest=
ic animals such
as cats and dogs, are more likely to develop sensitivity to laboratory an=
imals and asthma
than nonallergic workers [Bryant et al. 1995].
Studies of workers exposed to animals associate many occupations with =
an increased risk
of asthma and other respiratory symptoms [Lutsky et al. 1985; Zejda et al=
. 1993; Zuskin et
al. 1992a,b; Bar-Sela et al. 1984]. These occupations include laboratory =
animal workers,
veterinarians, livestock workers, garment workers, and horse handlers. Ri=
sks associated
with some of these occupations are outlined here.
Laboratory Animal Workers
Workers are exposed to laboratory animals in the pharmaceutical indust=
ry, university
laboratories, research units, and animal breeding facilities [Chan-Yeung =
and Malo 1994].
Most reactions to exposures in these facilities involve small animals suc=
h as rodents.
Reactions associated with primates, cats, dogs, and domestic farm animals=
have also been
reported [Lincoln et al. 1974].
Animal contact occurs during feeding, cleaning, dosing, sacrifice, sur=
gery, and body
fluid collection, measurement, and transport through the facilities [Harr=
ies and Cromwell
1982]. Workers are exposed to animal dander, hair, urine, saliva, tissues=
, and sera
[Harries and Cromwell 1982].
Animal-related allergy is one of the most important health hazards enc=
ountered by
laboratory animal workers [Newman-Taylor and Gordon 1993]. Health surveys=
of persons
currently working with laboratory animals indicate that up to 56% are aff=
ected by
animal-related allergies [Aoyama et al. 1992; Bardana 1992; Bryant et al.=
1995; Hunskaar
and Fosse 1993; Kibby et al. 1989; Lutsky et al. 1985; Newill et al. 1986=
; Zejda et al.
1993]. In a survey of 5,641 workers from 137 animal facilities, 23% had a=
llergic symptoms
related to laboratory animals. Of the workers with symptoms, 82% had nasa=
l or eye
symptoms, 46% had skin complaints, and 33% had asthma. These figures do n=
ot include former
workers who became ill and could not continue to work.
Veterinarians and Veterinary Technicians
Increased prevalences of asthma, respiratory infections, and obstructi=
ve lung disease
have been observed in veterinarians. Those who work with large animals se=
em to have fewer
problems with asthma and allergies than those who work with small animals=
[Lutsky et al.
1985].
Livestock Workers
Rhinitis and occupational asthma are recognized effects of working wit=
h livestock such
as cattle, hogs, sheep, and goats. Hog producersCparticularly
those who work in large confinement areas with inadequate ventilationChave been shown to develop wheez=
ing and chronic
coughing [Zejda et al. 1993; Zuskin et al. 1992b].
Garment Workers
Workers in the garment industry may have allergic reactions to pelts a=
nd fur as well as
to textiles made from animal products such as wool, cashmere, alpaca, vic=
una, and mohair
[Bardana 1992].
Horse Handlers
Horse exposure poses a risk to agricultural workers, mounted law enfor=
cement units, and
race track and stable attendants.
HEAL=
TH
EFFECTS
Exposure to airborne animal allergens may at first result in nasal, ey=
e, and throat
irritation as well as skin hives [Ohman 1978; Lincoln et al. 1974]. As ma=
ny as 50% of
workers with these symptoms go on to develop asthma symptoms such as recu=
rrent episodes of
coughing, wheezing, chest tightness, and difficult breathing [Bardana 199=
2]. Nasal
symptoms usually develop first; occupational asthma without nasal symptom=
s is uncommon.
Once an individual has become sensitized to animals, allergy symptoms can=
occur after only
a few minutes of exposure, or they may be delayed up to 8 hours or more. =
In severe cases,
anaphylactic reactions (including shock) may develop, although rarely.
Symptoms of asthma may first appear long after beginning work with ani=
mals. Laboratory
animal allergy usually develops within 36 months of starting exposure, an=
d most cases
develop after 6 to 36 months of exposure. Animal workers who do not becom=
e allergic within
3 years of exposure are less likely to develop the problem after longer e=
xposures [Aoyama
et al. 1992]. However, a study of 16 poultry workers with symptoms of ast=
hma and rhinitis
showed that the onset of symptoms can be delayed for up to 10 years [Bar-=
Sela et al.
1984].
After exposure is terminated, the nasal and eye symptoms often disappe=
ar shortly, but
the lung symptoms tend to persist [Newman-Taylor and Gordon 1993]. In the=
poultry workers,
nasal symptoms and asthma were persistent even after affected workers lef=
t the poultry
house [Bar-Sela et al. 1984].
CASE
REPORTS
Case 1 - Exposure
to Laboratory Rats
A 21-year-old female worker at a pharmaceutical company prepared rats =
for experiments.
She had no prior respiratory illnesses, but she had a family history of a=
llergies. Three
months after she started working, the worker noted hives on her forearms =
and hands. Her
symptoms worsened until every direct contact with rats produced hives. We=
aring gloves
alleviated the problem, but she could not perform her work adequately whe=
n using them.
The worker then began to suffer episodes of sneezing, nasal drainage, =
watery eyes, and
chest tightness. She was transferred to another department, where her sym=
ptoms ceased.
However, they recurred if she entered a room with rats or where rats had =
previously been
housed. The worker had positive skin tests to animal dander and to rat ha=
ir. She also had
elevated antibodies (IgE) to various rat proteins [DeGroot and Messerschm=
idt 1984].
Case 2 - Exposure to
Rabbits
A 32-year-old physician had been working on a research project involvi=
ng rabbits for 22 years. He h=
ad an allergy to cats but not to dust
mites or other common allergens. The physician developed progressively wo=
rsening nasal
congestion and eye irritation. During work with a rabbit, he received an =
accidental
needlestick. Within 15 minutes, the physician noted progressive itching, =
swelling of the
face, hives, throat tightness, and inability to speak. He was admitted to=
the hospital
where he received emergency treatment for anaphylactic shock. His symptom=
s stabilized over
a 5-hour period. Blood samples showed increased antibodies (IgE) to cat d=
ander and rabbit
epithelium. The antibodies to rabbit epithelium declined over the 6-month=
period after he
left the job that involved rabbit contact [Watt and McSharry 1996].
Case 3 - Exposure to
Various Animals
Thirty-eight students were examined during their first year of trainin=
g as laboratory
technicians (median age was 21 years). They were re-examined after workin=
g with various
laboratory animals (primarily rats, mice, and rabbits) for an average of =
18 months. At
that time, nine students (24%) had developed allergies to laboratory anim=
als. Symptoms
included nasal and eye irritation in seven students, skin rashes in four,=
and chest
problems in three. Of the nine students with animal allergies, seven had =
reactions to rat
or mouse antigen in skin-prick tests, and eight showed asthma-like reacti=
ons during lung
testing [Renstr=F6m et al. 1995].
CONC=
LUSIONS
Asthma and other respiratory illnesses may develop in persons whose wo=
rk requires close
contact with animals and animal products. Asthma in animal handlers can r=
esult in
respiratory symptoms that are severe and persistent. These symptoms can l=
ead to permanent
disability or require a career change. Workers at risk for developing sym=
ptoms should be
advised to take precautions to avoid or minimize exposures. Several metho=
ds for reducing
exposures and decreasing the risk of sensitization are available to emplo=
yers and workers.
Medical monitoring of exposed workers and remedial actions for workers wi=
th symptoms can
further reduce the risk of permanent adverse health effects. With timely =
and appropriate
action, many cases of animal-related asthma can be prevented.
RECO=
MMENDATIONS
NIOSH recommends the following measures to reduce exposures to animal =
allergens in the
workplace and prevent animal-induced asthma and allergies:
1. Modify ventilation and filtration systems:
Increase the ventilation rate and humidity in the animal-hous=
ing areas.
Ventilate animal-housing and -handling areas separately from the=
rest of the facility.
Direct airflow away from workers and toward the backs of the animal=
cages.
Install ventilated animal cage racks or filter-top animal cages.
2. Perform animal manipulations within ventilated hoods or safety cabi=
nets when
possible.
3. Decrease animal density (number of animals per cubic meter of room =
volume).
4. Avoid wearing street clothes while working with animals. Leave work=
clothes at the
workplace to avoid potential exposure problems for family members.
5. Keep cages and animal areas clean. Take particular care to control =
exposures during
cleaning.
6. Use absorbent pads for bedding. If these are unavailable, use cornc=
ob bedding
instead of sawdust bedding.
7. Use an animal species or sex that is known to be less allergenic th=
an others.
8. Reduce skin contact with animal products such as dander, serum, and=
urine by using
gloves, lab coats, and approved particulate respirators with faceshields.=
9. Provide training to educate workers about animal allergies and step=
s for risk
reduction.
10. Provide health monitoring and appropriate counseling and medical f=
ollowup for
workers who have become sensitized or have developed allergy symptoms.
These recommendations are discussed briefly in the following subsectio=
ns.
Environmental Factors
Exposures to airborne allergens are affected by patterns of air flow, =
air filtration,
bedding type, and humidity [Newman-Taylor and Gordon 1993]. Manipulating =
such
environmental factors has successfully reduced or eliminated the risk of =
animal-induced
allergies [Ohman 1978]. For example, patterns of room ventilation can be =
manipulated to
reduce workplace exposures to animal allergens. Recirculated airflow shou=
ld be avoided
unless it is well filtered to remove animal dander and odors [Lincoln et =
al. 1974; Ohman
1978]. Increasing the ventilation rate and humidity decreases the amount =
of rat urine
protein in laboratory air [Newman-Taylor and Gordon 1993; Hunskaar and Fo=
sse 1993].
Allergen exposures are also reduced by performing animal manipulations wi=
thin ventilated
hoods in safety cabinets, by directing airflow away from the worker and t=
oward the backs
of the cages, and by using ventilated animal cage racks or filter-top cag=
es [Lincoln et
al. 1974].
To prevent the dispersion of allergens, street clothes should not be w=
orn while working
with animals. Potential problems for family members can be minimized by s=
toring and
laundering work clothes at the workplace [Ohman 1978; Lincoln et al. 1974=
].
Animal Maintenance Factors
The following animal maintenance factors influence the worker's airbor=
ne exposures to allergens:
Animal density (the number of animals per cubic meter of room vol=
ume)
Activity (sweeping and cleaning of cages, which can result in very hi=
gh exposures)
Cage design
Bedding type [Eggleston and Wood 1992; Newman-Taylor and Gordon 1993;=
Bardana 1992]
The elimination of sawdust bedding and the use of absorbent pads as be=
dding material
have been shown to reduce concentrations of allergens in the air [Gordon =
et al. 1992]. If
absorbent pads are unavailable, corncob bedding is preferable to sawdust =
bedding
[Sakaguchi et al. 1990; Edwards et al. 1983]. Vacuum cleaners or ventilat=
ion benches
should always be used when cleaning cages to avoid airborne exposure.
Less Allergenic Animals
Some animals appear to produce allergic reactions in workers more freq=
uently than
others. For example, male rats are more allergenic than female rats, and =
rats are more
allergenic than rabbits. Using a less allergenic species or sex can help =
reduce risks
[Hunskaar and Fosse 1993; Newman-Taylor and Gordon 1993; Bardana 1992].
Skin Contact
Avoiding skin contact with animal products such as animal dander, seru=
m, and urine has
no proven benefit, but it may decrease the risk of sensitization. Gloves,=
lab coats, and
approved particulate respirators with faceshields can all decrease skin e=
xposure [Lincoln
et al. 1974; Newman-Taylor and Gordon 1993; Bardana 1992].
Training
Workers at risk of developing animal-related asthma or allergies shoul=
d be offered
training that reviews the type and timing of typical symptoms, the import=
ance of early
detection and intervention, and steps that workers and managers can take =
to reduce the
risk of sensitization.
Medical Monitoring and Surveillance=
Ongoing medical monitoring for symptoms of asthma may help protect the=
health of animal
handlers. Medical screening with standardized questionnaires can identify=
workers with
early symptoms of asthma [Venables et al. 1993]. Workers who report sympt=
oms related to
their jobs (sneezing, runny nose, chest tightness, wheezing, and episodes=
of cough or
shortness of breath) should be referred for more extensive evaluation and=
early
intervention, as appropriate. Current knowledge suggests that early termi=
nation of animal
exposure for workers with asthma symptoms can reduce their risk of develo=
ping long-term
symptoms. Spirometry and blood antibody testing have also been used in me=
dical monitoring
for asthma, but their exact roles are not yet defined. NIOSH has develope=
d a surveillance
case definition for occupational asthma (see Appendix). This definition m=
ay help guide
medical evaluations.
Some workers with animal-related asthma and allergies may improve or c=
ompletely resolve
their symptoms, whereas others may have persistent symptoms. Several fact=
ors affect this
outcome. Individuals are more likely to do poorly if they
—have their symptoms for a long period before the condition is recog=
nized,
—have severe disease at diagnosis (as indicated by lung function and=
airway
responsiveness tests), or
—have a long period of exposure before developing symptoms [Venables=
and
Chan-Yeung 1997; Paggiaro et al. 1994].
Thus delays in recognizing the condition or stopping the exposure may =
result in more
severe and persistent lung disease and disability [Brooks 1992].
Some employers have used preplacement examinations to identify workers=
at high risk for
developing animal-related asthma and allergies. However, no evidence indi=
cates that the
use of a particular criterion will predict which workers will become alle=
rgic or develop
animal-related asthma and allergies [Aoyama et al. 1992; Kibby et al. 198=
9]. Workers with
a history of allergic disease are at increased risk, but this criterion i=
s not useful for
screening. For example, in one group of workers using the questionnaire a=
s a screening
tool, only 3 of 12 workers with a history of allergy developed animal-ind=
uced asthma. The
screening criterion would have excluded nine workers who did not develop =
the problem
[Kibby et al. 1989].
The presence of antibodies (IgE) in a worker's=20
serum also fails to correlate with the presence of symptoms or the develo=
pment of disease. Pre-employment screening for allergy risk factors is t=
herefore not warranted [Aoyama et al. 1992].
Appropriate Counseling for Affected Workers=
Occupational asthma symptoms must be recognized early and affected wor=
kers must be
removed from exposure to allergens, since prolonged exposure can lead to =
irreversible
disease. However, removal from exposure does not always lead to complete =
recovery
[Venables and Chan-Yeung 1997]. Only about 50% of those with occupational=
asthma from
various causes recover completely after exposures are ended [Brooks 1992]=
.
Some workers may be unwilling to leave their jobs in spite of health p=
roblems. A worker
who has severe or life-threatening allergic reactions should be strongly =
advised to change
jobs, since no prevention strategy is completely effective [Newman-Taylor=
and Gordon
1993].
Workers with symptoms who wish to continue working with animals should=
be advised of
the risks. Careful medical monitoring is necessary to assure adequate con=
trol of the
illness. Strict use of approved particulate respirators (as part of a for=
mal respiratory
protection program), prudent work practices, and careful housekeeping may=
allow a person
with mild asthma to continue working [Brooks 1992]. However, the routine =
use of
respirators is not recommended as an allergen control technique [Lincoln =
et al. 1974]. If
extensive medical treatment (for example, the use of steroid tablets) is =
required, or if
repeated asthma attacks occur after all steps have been taken to reduce e=
xposure, the
affected worker should leave the offending job.
Surveillance and Disease Reporting
NIOSH encourages the surveillance of occupational asthma by State heal=
th departments.
To encourage uniform reporting, NIOSH recommends reporting guidelines and=
an asthma
surveillance case definition (see Appendix). These guidelines and the cas=
e definition are
recommended for public health surveillance of work-related asthma reporte=
d by physicians
and other health care providers. As of 1998, three State health departmen=
tsCCalifornia, Massachusetts,=
and MichiganCare funded by N=
IOSH for asthma surveillance
activities.
ACKN=
OWLEDGMENTS
The principal contributors to this Alert were Gina Buono, Bradley Husb=
erg, Hector
Ortega, Edward Petsonk, Dorothy Tan-Wilhelm, and John Parker. Please dire=
ct comments,
questions, or requests for additional information to the following:
Director
Division of Respiratory Disease Studies
NIOSH
1095 Willowdale Road
Morgantown, WV 26505-2888
Telephone, (304) 285-5750; or call 1-800-35-NIOSH (1-800-356-4674).
We greatly appreciate your assistance in protecting the lives of U.S. =
workers.
Linda Rosenstock, M.D., M.P.H.
Director, National Institute for Occupational Safety and Health
Centers for Disease Control and Prevention
REFE=
RENCES
Aoyama K, Ueda A, Manda F, Matsushita T, Ueda T, Yamauchi C [1992]. Al=
lergy to
laboratory animals: an epidemiological study. Br J Ind Med 49(1):4=
1-47.
Bardana EJ Jr. [1992]. Occupational asthma and related conditions in a=
nimal workers.
In: Bardana EJ Jr., Montanaro A, O'Hollaren MT,
eds. Occupational asthma. Philadelphia, PA: Hanley & Belfus.
Bar-Sela S, Teichtahl H, Lutsky I [1984]. Occupational asthma in poult=
ry workers. J
Allergy Clin Immunol 73(2):271-275.
Beckett WS [1994]. The epidemiology of occupational asthma. Eur Respir=
J 7:161-164.
Brooks SM [1992]. Occupational and environmental asthma. In: Rom WN, e=
d. Environmental
and occupational medicine. 2nd ed. Boston, MA: Little, Brown and Company,=
pp. 393-446.
Bryant DH, Boscato LM, Mboloi PN, Stuart MC [1995]. Allergy to laborat=
ory animals among
animal handlers. Med J Aust 163(8):415-418.
Chan-Yeung M, Malo JL [1994]. Aetiological agents in occupational asth=
ma. Eur Respir J 7:346
-371.
DeGroot AC, Messerschmidt HJM [1984]. Laboratory animal allergy. Conta=
ct Dermatitis 11(2):121-122.
Edwards RG, Beeson MF, Dewdney JM [1983]. Laboratory animal allergy: t=
he measurement of
airborne urinary allergens and the effects of different environmental con=
ditions. Lab Anim
17(3):235-239.
Eggleston PA, Wood RA [1992]. Management of allergies to animals. Alle=
rgy Proc 13(6):289-292.
Gordon S, Tee RD, Lowson D, Wallace J, Newman Taylor AJ [1992]. Reduct=
ion of airborne
allergenic urinary proteins from laboratory rats. Br J Ind Med 49(=
6):416-422.
Harries MG, Cromwell O [1982]. Occupational asthma caused by allergy t=
o pigs' urine. Br Med J Clin =
Res 284(6319):867.
Hollander A, Heederik D, Doekes G [1997]. Respiratory allergy to rats:
exposure-response relationships in laboratory animal workers. Am J Respir=
Crit Care Med 155:562-567.
Hunskaar S, Fosse RT [1993]. Allergy to laboratory mice and rats: a re=
view of its
prevention, management, and treatment. Lab Anim 27(3):206-221.
Kibby T, Powell G, Cromer J [1989]. Allergy to laboratory animals: a p=
rospective and
cross-sectional study. J Occup Med 31(10):842-846.
Lincoln TA, Bolton NE, Garrett AS Jr. [1974]. Occupational allergy to =
animal dander and
sera. J Occup Med 16(7):456-469.
Lutsky I, Baum GL, Teichtahl H, Mazar A, Aizer F, Bar-Sela S [1985]. O=
ccupational
respiratory disease in veterinarians. Ann Allergy 55:153-156.
Newill CA, Evans III R, Khoury MJ [1986]. Preemployment screening for =
allergy to
laboratory animals: epidemiologic evaluation of its potential usefulness.=
J Occup Med 28(11):1158-1164.
Newman-Taylor AJ, Gordon S [1993]. Laboratory animal and insect allerg=
y. In: Bernstein
IL, Chan-Yeung M, Malo J-L, Bernstein DI, eds. Asthma in the workplace. N=
ew York, NY:
Marcel Dekker, Inc., pp. 399-414.
NHLBI (National Heart, Lung, and Blood Institute) [1995]. Global initi=
ative for asthma.
Global strategy for asthma management and prevention. NHLBI/WHO Workshop =
Report. Bethesda,
MD: U.S. Department of Health and Human Services, Public Health Service, =
National
Institutes of Health, NIH Publication No. 95-3659.
Ohman JL Jr. [1978]. Allergy in man caused by exposure to mammals. J A=
m Vet Med Assoc 172(1):1403-1406.
Paggiaro PL, Vagaggini B, Bacci E, Bancalari L, Carrara M, Di Franco A=
, et al. [1994].
Prognosis of occupational asthma. Eur Respir J 7:761-767.
Parker JE, Petsonk EL, Weber SL [1992]. Hypersensitivity pneumonitis a=
nd organic dust
toxic syndrome. Immunol and Allergy Clin of North America 12(2):27=
9-290.
Renstr=F6m A, Maimberg P, Larsson K, Sundblad B-M [1995]. Allergic sen=
sitization is
associated with increased bronchial responsiveness: a prospective study o=
f allergy to
laboratory animals. Eur Respir J 8:1514-1519.
Sakaguchi M, Inouye S, Miyazawa H, Kamimura H, Kimura M, Yamazaki S [1=
990]. Evaluation
of countermeasures for reduction of mouse airborne allergens. Lab Anim Sc=
i 40(6):613-615.
Salvaggio JE, Taylor G, Weill H [1986]. Occupational asthma and rhinit=
is. In: Merchant
JA, ed. Occupational respiratory diseases. Cincinnati, OH: U.S. Departmen=
t of Health and
Human Services, Public Health Service, Centers for Disease Control, Natio=
nal Institute for
Occupational Safety and Health, DHHS (NIOSH) Publication No. 86-102, pp. =
461-477.
Venables KM, Chan-Yeung M [1997]. Occupational asthma. Lancet 349:1465-1469.
Venables KM, Farrer N, Sharp L, Graneek BJ, Newman-Taylor AJ [1993]. R=
espiratory
symptoms questionnaire for asthma epidemiology: validity and reproducibil=
ity. Thorax 48(3):214-219.
Watt AD, McSharry CP [1996]. Laboratory animal allergy: anaphylaxis fr=
om a needle
injury. Occup Environ Med 53:573-574.
Zejda JE, Hurst TS, Rhodes CS, Barber EM, McDuffie HH, Dosman JA [1993=
]. Respiratory
health of swine producers. Focus on young workers. Chest 103(3):70=
2-709.
Zuskin E, Kanceljak B, Stilinovic L, Schachter EN, Kopjar B [1992a]. I=
mmunological
status and respiratory findings in furriers. Am J Ind Med 21(3):43=
3-441.
Zuskin E, Zagar Z, Schachter EN, Mustajbegovic J, Kern J [1992b]. Resp=
iratory symptoms
and ventilatory capacity in swine confinement workers. Br J Ind Med 49=
(6):435-440.
APPE=
NDIX
NIOSH Surveillance Guidelines for State Health Departments: Occup=
ational Asthma*,†
Reporting Guidelines=
State health departments should encourage providers to =
report all
suspected or diagnosed cases of occupational asthma. These should include=
persons with
A. A physician's diagnosis of
asthma
AND
B. An association between symptoms of asthma and work.
State health departments should collect appropriate cli=
nical,
epidemiologic, and workplace information about reported cases to set prio=
rities for
workplace investigations.
Surveillance Case Definition
A. A physician's diagnosis of
asthma
AND
B. An association between symptoms of asthma and work a=
nd any one of the
following:
1. Workplace exposure to an agent or process previously=
associated with
occupational asthma,
OR
2. Significant work-related changes in forced expirator=
y volume in 1
second (FEV1) or peak expiratory flow rate (PEFR),
OR
3. Significant work-related changes in airway responsiv=
eness as measured
by nonspecific inhalation challenge,
OR
4. Positive response to inhalation provocation testing =
with an agent to
which the patient is exposed at work. Inhalation provocation testing with=
workplace
substances is potentially dangerous and should be performed by experience=
d personnel in a
hospital setting where resuscitation facilities are available and where f=
requent
observations can be made over sufficient time to monitor for delayed reac=
tions.
*Asthma is a chronic inflammatory disorder of the=
airways in which
many cells and cellular elements play a role. In susceptible individuals,=
this
inflammation causes recurrent episodes of wheezing, breathlessness, chest=
tightness, and
coughing, particularly at night or in the early morning. These episodes a=
re usually
associated with widespread but variable airflow obstruction that is often=
reversible,
either spontaneously or with treatment. This inflammation also causes an =
associated
increase in the existing bronchial hyperresponsiveness to a variety of st=
imuli [NHLBI
1995]. This hyperresponsiveness may be demonstrated by significant change=
s in the forced
expiratory volume in 1 second (FEV1) or peak expiratory flow rate (PEFR).=
Airflow changes
can occur spontaneously with treatment, with a precipitating exposure, or=
with diagnostic
maneuvers such as nonspecific inhalation challenge.
Patterns of association can vary. The following example=
s are patterns that
may suggest an occupational etiology:
Symptoms of asthma develop after a worker start=
s a new job or after new
materials are introduced on a job (a substantial period of time may=
elapse between initial
exposure and development of symptoms).
Symptoms develop within minutes of specific act=
ivities or exposures at
work.
Delayed symptoms occur several hours after expo=
sure, during the evenings
of workdays.
Symptoms occur less frequently or not at all on=
days away from work and
on vacations.
Symptoms occur more frequently on returning to =
work.
Work-related changes in medication requirements may hav=
e similar patterns,
also suggesting an occupational etiology.
Many agents and processes have been associated with occ=
upational asthma
[Chan-Yeung and Malo 1994; Salvaggio et al. 1986], and others continue to=
be recognized.
Changes in nonspecific bronchial hyperactivity can be measured by serial =
inhalation
challenge testing with methacholine or histamine. Increased bronchial rea=
ctivity
(manifested by reaction to lower concentrations of methacholine or histam=
ine) following
exposure and decreased bronchial reactivity after a period away from work=
are evidence of
work-relatedness.
†Reprinted from CDC [1990], p. 43.
DISCLAIMER
Mention of any company or product does not constitute endorsem=
ent by the National
Institute for Occupational Safety and Health.
This document is in the public domain and may be freel=
y
copied or reprinted.
Copies of this and other NIOSH documents are availabl=
e from
Publications Dissemination, EID
National Institute for Occupational Safety and Health
4676 Columbia Parkway
Cincinnati, OH 45226-1998
Fax number: (513) 533-8573
Telephone number: 1-800-35-NIOSH
(1-800-356-4674)
E-mail: pubstaft@
To receive other information about occupational safet=
y and health
problems,
call 1-800-35-NIOSH (1-800-356-4674),
or visit the NIOSH Homepage
on the World Wide Web at
DHHS (NIOSH) Publicat=
ion No. 97-116
January 1998=
This page was last updated on February 13, 1998
--------------BB91C53F8AB1EA0002D10766--
=========================================================================
Date: Fri, 8 Jan 1999 08:47:38 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jairo Betancourt
Subject: Re: Eye/Face Protection While Handling Non-Human Primates
MIME-version: 1.0
Content-type: text/plain; charset="iso-8859-1"
Content-transfer-encoding: 7bit
Hi David!: We had a research project with three species of monkeys and
Mycobacterium tuberculosis. The monkeys were always "knock out" before any
handling procedure, such as surgery, broncoscopy, etc. It does not have any
influence on the research results. However, our scientists wore al the PPE
necessary, such as N95 masks, etc.and all personnel entering our holding
facility (BSL3) had to wear the appropriate masks. Thank you!
-----Original Message-----
From: DAVID STRUEBEL
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Thursday, January 07, 1999 5:33 PM
Subject: Eye/Face Protection While Handling Non-Human Primates
> I imagine that nearly all facilities using human human primates have
> re-addressed the eye protection requirments for handling these
> animals in light of the Herpes B related fatality in late 1997.
>
> We have attempted several approachs, none of which totally resolve the
> issue.
>
> Orignally our animal caretakers were required to wear safety glasses
> under a full face shield when handling or manipulating the animals.
> In many cases they were also wearing surgical masks.... One of the
> first things we did was to require the use of a NIOSH N95 or HEPA
> respirator.
>
> Realizing that the safety glasses even with the face shield did not
> provide adequate splash protection to the eye, we suggested the use of
> goggles. This has not been accepted very well due to fogging
> problems (yes, we tried anti-fog and vented goggles) plus the
> reduction in peripheral vision caused by the goggles).
>
> In some areas with a high probablity of aersol generation such a
> during necropsy procedures we have used a Powered Air Purifying
> Repirator (PAPR) but they are rather bulky.
>
> I need to add that we routinely do not anestitize our animals for
> dosing or examination. We have a particular problem with animal eye
> examinations by an Opthmalogist where the physician has to physically
> look into the animal's eyes at a short distance. We have thought
> about perhaps fabricationg a restraining device the would hold the
> animal's head in position for the physician to examine the eye
> through a slotted area.
>
>
> What are others doing about this issue?
>
> What type of eye/face/splash protection do you require while handling
> non-human primates?
>
> What PPE are you requiring for necropsy operations.
>
> Also advice on whether you put your animals down for exams or dosing,
> and whether this is practical on a large scale study.... Do you think
> the use of the anesthtetic will affect the outcome of the study?
=========================================================================
Date: Fri, 8 Jan 1999 09:08:53 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Heather Gonsoulin
Subject: Re: Eye/Face Protection While Handling Non-Human Primates
MIME-Version: 1.0
Content-Type: multipart/mixed; boundary="---- =_NextPart_000_01BE3AE6.85216540"
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Well, have you brought up a very timely issue! We are going through the =
process of determining if we should change our current policies on =
eye/face protection. I have been contacting some of the regional =
primate centers to find out what they are doing regarding eye/face =
protection. It seems that there is quite a range of eye protection =
policies out there. At our facility, we are wearing safety glasses and =
the N95 respirators everywhere except with alert studies, necropsies and =
dental procedures. When doing anything with an alert animal or during a =
necropsy or dental procedure, a faceshield is also worn with the safety =
glasses. At some of the regional primate centers the policies range =
from faceshield and goggles at all times to faceshield and safety =
glasses only when working with macaques. I know there will be a meeting =
between the CDC and the regional primate centers at the end of January =
to discuss this very subject. =20
And for the question about sedation or anesthesia, we do studies both =
ways and it does not affect the results in any way. Also, we do =
physicals on all of our 5000 primates at least once a year. During =
these physicals the animals are almost always sedated.
Now I have question for you. How many primates do you have and how many =
employees?
Heather H. Gonsoulin, RRA
Occupational Health and Safety Officer
USL-NIRC
(318) 482-0306 (fax) 318-373-0057
hah8377@usl.edu
-----Original Message-----
From: DAVID STRUEBEL [SMTP:DAVID.STRUEBEL@]
Sent: Thursday, 07 January, 1999 3:56 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Eye/Face Protection While Handling Non-Human Primates
I imagine that nearly all facilities using human human primates =
have
re-addressed the eye protection requirments for handling these
animals in light of the Herpes B related fatality in late 1997.
We have attempted several approachs, none of which totally resolve =
the
issue.
Orignally our animal caretakers were required to wear safety =
glasses
under a full face shield when handling or manipulating the animals.
In many cases they were also wearing surgical masks.... One of the
first things we did was to require the use of a NIOSH N95 or HEPA
respirator.
Realizing that the safety glasses even with the face shield did not
provide adequate splash protection to the eye, we suggested the use =
of
goggles. This has not been accepted very well due to fogging
problems (yes, we tried anti-fog and vented goggles) plus the
reduction in peripheral vision caused by the goggles).
In some areas with a high probablity of aersol generation such a
during necropsy procedures we have used a Powered Air Purifying
Repirator (PAPR) but they are rather bulky.
I need to add that we routinely do not anestitize our animals for
dosing or examination. We have a particular problem with animal =
eye
examinations by an Opthmalogist where the physician has to =
physically
look into the animal's eyes at a short distance. We have thought
about perhaps fabricationg a restraining device the would hold the
animal's head in position for the physician to examine the eye
through a slotted area.
What are others doing about this issue?
What type of eye/face/splash protection do you require while =
handling
non-human primates?
What PPE are you requiring for necropsy operations.
Also advice on whether you put your animals down for exams or =
dosing,
and whether this is practical on a large scale study.... Do you =
think
the use of the anesthtetic will affect the outcome of the study?
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------ =_NextPart_000_01BE3AE6.85216540--
=========================================================================
Date: Fri, 8 Jan 1999 12:53:24 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: Eye/Face Protection While Handling Non-Human Primates
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
David:
Here is a Biohazards Alert that I prepared for my clients concerning
this issue. I pass it on to yo in hopes that it will help. Joe Coggin.
BIOHAZARD ALERT
Date: January 6, 1999
To: University Safety Associates, Inc Clients
From: Joseph H. Coggin, Jr. Ph.D., RBP, CBSP
Professor and Chair, USA College of Medicine
Phone no. (334) 460-6314; Fax no. (334) 460-7269
Re: Interim recommendations from CDC&P, NIH and OSHA
regarding worker protection against accidental eye/ mucocutaneous
exposure to Cercopithecine herpesvirus [Herpes B virus]. See attached
copy of MMWR Vol. 47, No. 49 Dec. 1998, pp. 1073- 1076.
This report in the MMWR details the events leading to the death
on December 10, 1997 of a 22 year old animal worker who was exposed
mucocutaneously through her right eye to fecal material contamination
emanating from an unidentified, free-ranging Rhesus macaque which was
being moved to another cage. The report states that she was not required
to wear eye protection during this routine activity by the Yerkes Primate
Center which considered such activity a "low-risk" work situation for
exposure to B virus. The following additional "mistakes" were outlined
from the OSHA requested study that followed her death, some 42 days post-
exposure:
1) She wiped here eye with a paper towel to remove the splashed fecal
material;
2) She waited 45 minutes before irrigating the eye with tap water and
then only flushed the eye for 2-3 minutes;
3) The incident was not reported immediately;
4) The specific monkey source(s) responsible was not identified for B
virus testing;
5) Her eye was inflammed some 10 days later and only then did she seek
medical treatment at the emergency room [ER], first revealing the
exposure incident to her employer;
6) The attending I.D. physician was only consulted by phone, and assumed
that infection with B virus was unlikely based on the data base in the
Yerkes facility ER protocol. The I.D. physician was not available for an
immediate eye examination and evaluation;
7) Sulfonamide treatment was given by the attending ER physician lacking
the availability of an I.D. physician to see the patient;
8) Fearing a serious infection, she then consulted with her private
physician to get follow up on the inflamed eye;
9) She was referred to a private ophthalmologist who suspected cat
scratch fever initiated by a scratch from a pet cat and treated the eye
with doxycycline;
10) She sought additional medical treatment from yet another
ophthalmologist 2 days later after noting the beginning of intense pain,
neurological and other pronounced symptoms. She was hospitalized. She
was subsequently treated for possible Herpes B infection with Acyclovir
for two days, then treated continuously with Ganciclovir in the hospital
followed by short-course plasmapharesis and steroids which produced
temporary improvement. She was release from the hospital. She remained
Herpes B virus negative when her eye fluid/ tissue was tested. Ten days
later she reentered the hospital with ascending paralysis. She died on
December 10, 1997 of refractory respiratory failure. While she was
culture negative for B virus through November 13, 1997, she converted to
seropositivity to herpes B virus in Western blot testing.
Interim Recommendations to Prevent Ocular Splash and Mucocutaneous Exposures.
1. Prevent exposure by mandating regular and proper use of eye
protection and other facial mucous membrane personal protective equipment
[PPE] for all personnel entering a NH Primate facility in any areas where
primates or their wastes are present.
Note: Previous CDC, NIH and OSHA recommendations for preventing such
exposures had included protection with face shields, or surgical masks
with goggles or safety glasses [with side shields] when handling uncaged
active macaques. Many primate facilities require the constant use of eye
protection when entering the monkey facility, cleaning cages, or doing
any activity therein. This seems entirely appropriate. Face shields
only provide secondary level eye protection. Hence, face shields should
be used in conjunction with safety glasses or goggles, preferably using
goggles with fog resistant lenses and models that preserve peripheral vision.
2. Specific institutional procedures must be drawn up to manage
accidental mucocutaneous exposures should they occur in spite of eye/
face protection mandated when working with any non-human primates or
their wastes in any activity. It is imperative to specify the
recommended IMMEDIATE cleansing all wounds, bites and scratches or
abraded skin, and the prompt flushing of eyes and mucous membranes for a
full 15 minutes following accidental exposures using approved equipment
and solutions. Care must be taken not to use towels to wipe out
contaminating material which may serve to enhance deposition of viruses
into delicate tissues. Written protocols for such emergency response
must be immediately available to all employees at risk for exposure.
Training in using the emergency first aid procedures must be given.
3. Exposed workers must have rapid and direct access to a local medical
consultant knowledgeable in occupational exposures to B virus and other
pathogens likely to be transmitted to humans by non-human primates. I
recommend that written protocols outlining correct first aid procedures,
animal and human testing required, and medical follow up be provided to
the local occupational medicine physician and to any consultant I.D.
physicians engaged to care for the exposed worker to insure medical "due
diligence" compliance.
4. Diagnostic specimens must be collected from the NH primate source and
human patient promptly, and immediately tested at the B Virus Research
and Resource Laboratory now located at Georgia State University in
Atlanta, GA. Patient specimens should be handled, packaged and shipped
as a significant biohazard noting that herpes B virus is possibly present
in the clinical material.
On
Thu, 7 Jan 1999, DAVID STRUEBEL wrote:
> I imagine that nearly all facilities using human human primates have
> re-addressed the eye protection requirments for handling these
> animals in light of the Herpes B related fatality in late 1997.
>
> We have attempted several approachs, none of which totally resolve the
> issue.
>
> Orignally our animal caretakers were required to wear safety glasses
> under a full face shield when handling or manipulating the animals.
> In many cases they were also wearing surgical masks.... One of the
> first things we did was to require the use of a NIOSH N95 or HEPA
> respirator.
>
> Realizing that the safety glasses even with the face shield did not
> provide adequate splash protection to the eye, we suggested the use of
> goggles. This has not been accepted very well due to fogging
> problems (yes, we tried anti-fog and vented goggles) plus the
> reduction in peripheral vision caused by the goggles).
>
> In some areas with a high probablity of aersol generation such a
> during necropsy procedures we have used a Powered Air Purifying
> Repirator (PAPR) but they are rather bulky.
>
> I need to add that we routinely do not anestitize our animals for
> dosing or examination. We have a particular problem with animal eye
> examinations by an Opthmalogist where the physician has to physically
> look into the animal's eyes at a short distance. We have thought
> about perhaps fabricationg a restraining device the would hold the
> animal's head in position for the physician to examine the eye
> through a slotted area.
>
>
> What are others doing about this issue?
>
> What type of eye/face/splash protection do you require while handling
> non-human primates?
>
> What PPE are you requiring for necropsy operations.
>
> Also advice on whether you put your animals down for exams or dosing,
> and whether this is practical on a large scale study.... Do you think
> the use of the anesthtetic will affect the outcome of the study?
>
=========================================================================
Date: Fri, 8 Jan 1999 15:56:46 -0500
Reply-To: dliberma@
Sender: A Biosafety Discussion List
From: dan liberman
Subject: Re: Eye/Face Protection While Handling Non-Human Primates
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
DAVID STRUEBEL wrote:
>
> I imagine that nearly all facilities using human human primates have
> re-addressed the eye protection requirments for handling these
> animals in light of the Herpes B related fatality in late 1997.
>
> We have attempted several approachs, none of which totally resolve the
> issue.
>
> Orignally our animal caretakers were required to wear safety glasses
> under a full face shield when handling or manipulating the animals.
> In many cases they were also wearing surgical masks.... One of the
> first things we did was to require the use of a NIOSH N95 or HEPA
> respirator.
>
> Realizing that the safety glasses even with the face shield did not
> provide adequate splash protection to the eye, we suggested the use of
> goggles. This has not been accepted very well due to fogging
> problems (yes, we tried anti-fog and vented goggles) plus the
> reduction in peripheral vision caused by the goggles).
>
> In some areas with a high probablity of aersol generation such a
> during necropsy procedures we have used a Powered Air Purifying
> Repirator (PAPR) but they are rather bulky.
>
> I need to add that we routinely do not anestitize our animals for
> dosing or examination. We have a particular problem with animal eye
> examinations by an Opthmalogist where the physician has to physically
> look into the animal's eyes at a short distance. We have thought
> about perhaps fabricationg a restraining device the would hold the
> animal's head in position for the physician to examine the eye
> through a slotted area.
>
> What are others doing about this issue?
>
> What type of eye/face/splash protection do you require while handling
> non-human primates?
>
> What PPE are you requiring for necropsy operations.
>
> Also advice on whether you put your animals down for exams or dosing,
> and whether this is practical on a large scale study.... Do you think
> the use of the anesthtetic will affect the outcome of the study?
I was advised by a pathologist from the Primate where the Young Lady was
exposed that OSHA has required them to wear goggles and a face shield
when working with animals and performing high risk activities. Such
activities include handling and transport of animals, changing cages and
cleaning cages, etc. Safety glasses with side shields and a face shield
are not sufficient.
Dan Liberman
=========================================================================
Date: Fri, 8 Jan 1999 16:00:20 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Nick Rinaldi
Subject: Job Ad San Diego
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
San Diego, CA - Health & Safety Coordinator
PLEASE DISTRIBUTE THIS MESSAGE:
Wanted: Health Physicist or Industrial Hygienist.
This job requires a person who:
Has a strong desire to develop their career in Health and Safety,
Has the ability to work independently in a productive small business
environment, and
Has an entrepreneurial spirit to develop new business opportunities.
An expanding San Diego consulting firm is hiring a full-time Health and Safety
Coordinator to service our industrial client base. The position requires 1-4
years experience and a B.S. or M.S. in Health Physics, Industrial Hygiene or
Environmental & Occupational Health. The candidate will be required to
provide
services in laboratory safety protocols, radiation safety, biological safety
and chemical safety. Strong written, communication, and training skills are
mandatory. The applicant must also be proficient user of Microsoft Word,
Excel,
Power Point, and Access.
Interested candidates should fax or email their resume and salary requirements
to Occupational Services, Inc.
Fax: (619) 558-5756
Email: admin@
- Nick Rinaldi
voice: (619) 558-6736
fax: (619) 558-6756
Occupational Services, Inc.
11230 Sorrento Valley Rd. Suite 160
San Diego, CA 92121
=========================================================================
Date: Fri, 8 Jan 1999 22:45:30 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Diane Fleming
Subject: Re: Mildew
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
I asked my son, Mark Fleming, who has been working with negatives, tapes etc
in audiovisual communications for about 10 years, and this is his reply.
Negatives from film can be rewashed and dried without any ill effects,
however mildew will most likely leave a residual mark of some kind.
There are film cleaners, mostly acetone based (I think) that can be
used.
As far as video tapes go.. I don't know what kind of cleaning they can
tolerate. My guess is that they are too fragile to put up with much
cleaning, although I don't see how mildew can cause a negative effect,
as long as the tape isn't stuck together. If I had a chance, I would try
forwarding and rewinding the tape in a seperate device and then copy the
tape to a new cassette through an inexpensive VCR (just in case it gets
messed up).
Hope this helps. I wouldn't mind a being in your warm country at this time of
year..
Diane O. Fleming, Ph.D, CBSP
Biosafety Consultant
15611 Plumwood Court
Bowie, MD 20716-1434
Tel 301-249-3951
FAX 301-239-8837
email Dimerck@
=========================================================================
Date: Mon, 11 Jan 1999 12:02:25 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: chemical decontamination of urine/feces
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
We need to come up with a way to decontaminate collected pig urine/feces
from pigs infected with a hydrophilic virus. Because of volume and liquid
state, autoclaving or incineration are not reasonable options. We would
like to find an appropriate disinfectant that can be added to the collection
pans. Any suggestions anyone has would be appreciated.
Julie A. Johnson, Biosafety Officer
Iowa State University
Environmental Health & Safety
118 Agronomy Lab
phone: 515-294-7657
fax: 515-294-9357
E-mail: jajohns@iastate.edu
=========================================================================
Date: Mon, 11 Jan 1999 11:12:11 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Alfred L. 'Al' Jin"
Subject: Re: chemical decontamination of urine/feces
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Julie,
This is just a thought, but without opening up a can of worms, have you
thought of disposing the material into the sanitary sewage system. Once the
material enters the system, most virus will not survive due to the
competitive environment. If you think this idea can work for ya, make sure
that the system has tertiary process that involves final chlorination of
effluent materials. (Please note some sewage systems are not design with
this final stage and disposal into the environment may definately have an
impact).
Alternatively, if this is going to be a long term project, how about
setting up a septic tank system to collect and digest the waste. This would
be clean and simple (excuse the pun).
Al Jin, BSO, IH, CBSP, MS, CM(AAM), M(ASCP),
Lawrence Livermore National Laboratory, Livermore, CA,
Hazards Control Department, MS-289,
925 423-7385, E-mail:Jin2@
>We need to come up with a way to decontaminate collected pig urine/feces
>from pigs infected with a hydrophilic virus. Because of volume and liquid
>state, autoclaving or incineration are not reasonable options. We would
>like to find an appropriate disinfectant that can be added to the collection
>pans. Any suggestions anyone has would be appreciated.
>
>Julie A. Johnson, Biosafety Officer
>Iowa State University
>Environmental Health & Safety
>118 Agronomy Lab
>phone: 515-294-7657
>fax: 515-294-9357
>E-mail: jajohns@iastate.edu
>
=========================================================================
Date: Mon, 11 Jan 1999 13:32:54 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Melissa Nellis
Subject: BMBL
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Does anyone know if there will be a new edition of the CDC/NIH publication
entitled "Biosafety in Microbiological and Biomedical Laboratories" coming
out anytime soon? We want to order the latest edition for classroom use
next quarter.
_______________________________________________________________
Melissa A. Nellis, MPH
Research Biosafety Specialist Phone:(612)626-5892
University of Minnesota FAX: (612)624-1949
Environmental Health and Safety nelli001@maroon.tc.umn.edu
Boynton Health Service W-140
410 Church St. SE
Minneapolis, Minnesota 55455 Website: dehs.umn.edu
_______________________________________________________________
=========================================================================
Date: Mon, 11 Jan 1999 14:40:59 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Adrian Felts
Subject: Re: BMBL
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Melissa,
I recently received the 1993 edition of the BMBL. I believe this is the
most current edition, but in case you'd like to check you can call the US
Govt Printing Office at (202) 512-2356. They should be able to tell you if
there is a more current edition available.
At 01:32 PM 1/11/99 -0500, you wrote:
>Does anyone know if there will be a new edition of the CDC/NIH publication
>entitled "Biosafety in Microbiological and Biomedical Laboratories" coming
>out anytime soon? We want to order the latest edition for classroom use
>next quarter.
>
>
>_______________________________________________________________
> Melissa A. Nellis, MPH
> Research Biosafety Specialist Phone:(612)626-5892
> University of Minnesota FAX: (612)624-1949
> Environmental Health and Safety nelli001@maroon.tc.umn.edu
> Boynton Health Service W-140
> 410 Church St. SE
> Minneapolis, Minnesota 55455 Website: dehs.umn.edu
>_______________________________________________________________
>
****************************************************************
Adrian Felts
Research Associate and Safety Officer
Anthony G. Gristina Institute for Biomedical Research
=========================================================================
Date: Mon, 11 Jan 1999 14:59:18 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Larry Thompson
Subject: Re: chemical decontamination of urine/feces
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
>This is just a thought, but without opening up a can of worms, have you
>thought of disposing the material into the sanitary sewage system. Once the
>material enters the system, most virus will not survive due to the
>competitive environment.
Julie,
Al has a good idea that is worth pursuing. One of the smaller pig units at
Cornell has been hooked to the municipal sewage system for many years. The
sewage plant initially needed information such as number of animals, amount
of fecal material, and amount of water (pens have automatic flushing system
under the slatted floor).
Depending on the size and location of the housing unit, however, hooking to
municipal may not be practical. Most of our remote locations have septic
systems only sized for the human use, ie toilets and showers, and so a
septic system may be easily overwhelmed by the animal fecal matter added to
it.
Larry
Larry J. Thompson, DVM PhD
Director of Biosafety
College of Veterinary Medicine
Cornell University
Upper Tower Road phone 607-253-3900
Ithaca, NY 14853 fax 607-253-3943
"There is no limit to the good you can do if you don't care who gets the
credit."
Gen. George Marshall
=========================================================================
Date: Mon, 11 Jan 1999 15:01:23 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: BMBL
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Melissa,
It is my understanding that a current copy of this document is available
online at the CDC or NIH website. I am sure that they will also annnounce
when a new edition is published. We now tell our people to consult the
online document. We have a link at our website to the CDC.
Hope this helps.
Bob
>Does anyone know if there will be a new edition of the CDC/NIH publication
>entitled "Biosafety in Microbiological and Biomedical Laboratories" coming
>out anytime soon? We want to order the latest edition for classroom use
>next quarter.
>
>
>_______________________________________________________________
> Melissa A. Nellis, MPH
> Research Biosafety Specialist Phone:(612)626-5892
> University of Minnesota FAX: (612)624-1949
> Environmental Health and Safety nelli001@maroon.tc.umn.edu
> Boynton Health Service W-140
> 410 Church St. SE
> Minneapolis, Minnesota 55455 Website: dehs.umn.edu
>_______________________________________________________________
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Mon, 11 Jan 1999 14:56:42 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janet Peterson
Subject: Re: BMBL
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Melissa,
There is definitely a new edition of BMBL in preparation, which I believe
is due to be published in February or March 1999. Jonathan Richmond at the
CDC would know the exact date. Janet
Melissa Nellis wrote:
> Does anyone know if there will be a new edition of the CDC/NIH publication
> entitled "Biosafety in Microbiological and Biomedical Laboratories" coming
> out anytime soon? We want to order the latest edition for classroom use
> next quarter.
>
> _______________________________________________________________
> Melissa A. Nellis, MPH
> Research Biosafety Specialist Phone:(612)626-5892
> University of Minnesota FAX: (612)624-1949
> Environmental Health and Safety nelli001@maroon.tc.umn.edu
> Boynton Health Service W-140
> 410 Church St. SE
> Minneapolis, Minnesota 55455 Website: dehs.umn.edu
> _______________________________________________________________
--
******************************************
Janet S. Peterson, CBSP
Biological Safety Officer
University of Maryland
Department of Environmental Safety
7505 Yale Avenue
College Park, MD 20742
Tel: 301/405-3975; Fax: 301/314-9294
******************************************
=========================================================================
Date: Mon, 11 Jan 1999 12:11:29 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: BMBL
MIME-Version: 1.0
Content-Type: text/plain
According to what Rich Knudsen of the CDC said at the ABSA meeting last
October, the new edition of the BMBL is due out this year, probably
fairly soon. Try calling Rich at 404-639-3235. Also, the NIH
Guidelines for Research Involving Recombinant DNA Molecules gets updated
fairly frequently and contains much information similar to that of the
BMBL but with an rDNA slant. Try accessing these doucments on-line
through the ABSA web site (.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Adrian Felts [mailto:afelts@OSF1.GMU.EDU]
Sent: Monday, January 11, 1999 11:41 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: BMBL
Melissa,
I recently received the 1993 edition of the BMBL. I believe this is the
most current edition, but in case you'd like to check you can call the
US
Govt Printing Office at (202) 512-2356. They should be able to tell you
if
there is a more current edition available.
At 01:32 PM 1/11/99 -0500, you wrote:
>Does anyone know if there will be a new edition of the CDC/NIH
publication
>entitled "Biosafety in Microbiological and Biomedical Laboratories"
coming
>out anytime soon? We want to order the latest edition for classroom
use
>next quarter.
>
>
>_______________________________________________________________
> Melissa A. Nellis, MPH
> Research Biosafety Specialist Phone:(612)626-5892
> University of Minnesota FAX: (612)624-1949
> Environmental Health and Safety nelli001@maroon.tc.umn.edu
> Boynton Health Service W-140
> 410 Church St. SE
> Minneapolis, Minnesota 55455 Website: dehs.umn.edu
>_______________________________________________________________
>
****************************************************************
Adrian Felts
Research Associate and Safety Officer
Anthony G. Gristina Institute for Biomedical Research
=========================================================================
Date: Mon, 11 Jan 1999 15:00:36 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Patricia Olinger
Subject: CHO Position
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: 7bit
Environment and Safety Professional Needed
Pharmacia & Upjohn Co., an international Pharmaceutical company, is
looking to hire a Safety/Industrial Hygiene Professional to support their
U.S. R&D, Kalamazoo, Michigan facility.
The incumbent will be responsible for the Chemical Hygiene Plan
implementation and maintenance in support of laboratory activities, assuring
the achievement of regulatory compliance objectives. Depending on
experience and background other activities may include: laboratory safety
training program development and industrial hygiene program support, RCRA,
facility planning and basic safety programs.
The incumbent must have the ability to interface within a wide variety of
situations and handle multiple tasks at one time. Proven written and oral
communication skills are a necessity as well as a strong background in
chemistry.
Previous experience as a CHO is highly desirable.
Interested candidates should fax or email their resume and salary
requirements to:
Pharmacia & Upjohn, Inc.,
Safety and Environment Services.
MS 7259-126-732 Attn: Patty Olinger
301 Henrietta St.
Kalamazoo, MI 49001
616-833-9488 FAX
616-833-7931 Voice
=========================================================================
Date: Mon, 11 Jan 1999 15:55:16 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Barbara Ernisse
Subject: Odor problems - Can you help?
In-Reply-To:
MIME-version: 1.0
Content-type: TEXT/PLAIN
Happy New Year.
I am hoping that the collective biosafety community can come up with some ideas
for a problem that is new to our building but apparently not uncommon...
We have a two tank water neutralizing system for the research building. All
water running from the research laboratory sinks is collected into an 800 gallon
tank, mixed, the pipet tips and other solids settle, and the water is gravity
fed to a second tank for pH adjustment before discharge to POTW. Water from
animal facilities and service labs in the basements gravity feeds to a secondary
tank system where it sits until the sump pump activates and pumps the water to
the main tanks.
About 10 months ago we began to experience a nasty odor. There is no
relationship between odor and use of chemicals for acid/base neutralizing.
There seems to be a relationship between low water flow and increased odor.
Engineering has done a great job adjusting ventillation and sealing the tanks to
contain the odor but the building operated without odor problems for years; we
are concerned about the onset of odors.
Various noses of course react differently. I think that the smell varies
between something dead and hay infusions. Whatever the odor is, it has
blackened the copper pipes that run through the area. It can be overwhelming.
Drain disposal of hazardous, noxious, and a long list of other chemicals is
forbidden in our location. We have walked the building during early morning
odor occurances and found no one in the labs
To get to the point, I am wondering if anyone has any experience in similar
problems. What was the root cause of the odor? Are there any strong microbial
suspects we can or should culture for? If you had this problem, did draining
and cleaning the tanks end the problem or was that a temporary fix?
Any help or direction will be gratefully accepted and considered.
Hope your 1999 smells better than mine--
Thanks
Barb Ernisse
Children's Hospital
(617)355-6230
[ernisse_b@a1.tch.harvard.edu}
=========================================================================
Date: Mon, 11 Jan 1999 15:24:23 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: Odor problems - Can you help?
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Sounds to me like you're doing a little anaerobic biodegradation down
there. Does it smell like rotten eggs? Hydrogen sulfide from sulfate
bacteria. Stinky feet? volatile organic chemicals from other microbs.
Maybe you better stop trying to seal it off and instead bubble some oxygen
into it. Either that or patent it and sell it to the oil patch.
Barbara Ernisse on 01/11/99 02:55:16 PM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: Odor problems - Can you help?
Happy New Year.
I am hoping that the collective biosafety community can come up with some
ideas
for a problem that is new to our building but apparently not uncommon...
We have a two tank water neutralizing system for the research building.
All
water running from the research laboratory sinks is collected into an 800
gallon
tank, mixed, the pipet tips and other solids settle, and the water is
gravity
fed to a second tank for pH adjustment before discharge to POTW. Water
from
animal facilities and service labs in the basements gravity feeds to a
secondary
tank system where it sits until the sump pump activates and pumps the water
to
the main tanks.
About 10 months ago we began to experience a nasty odor. There is no
relationship between odor and use of chemicals for acid/base neutralizing.
There seems to be a relationship between low water flow and increased odor.
Engineering has done a great job adjusting ventillation and sealing the
tanks to
contain the odor but the building operated without odor problems for years;
we
are concerned about the onset of odors.
Various noses of course react differently. I think that the smell varies
between something dead and hay infusions. Whatever the odor is, it has
blackened the copper pipes that run through the area. It can be
overwhelming.
Drain disposal of hazardous, noxious, and a long list of other chemicals is
forbidden in our location. We have walked the building during early
morning
odor occurances and found no one in the labs
To get to the point, I am wondering if anyone has any experience in similar
problems. What was the root cause of the odor? Are there any strong
microbial
suspects we can or should culture for? If you had this problem, did
draining
and cleaning the tanks end the problem or was that a temporary fix?
Any help or direction will be gratefully accepted and considered.
Hope your 1999 smells better than mine--
Thanks
Barb Ernisse
Children's Hospital
(617)355-6230
[ernisse_b@a1.tch.harvard.edu}
=========================================================================
Date: Mon, 11 Jan 1999 16:28:02 EST
Reply-To: speaker@ehs.psu.edu
Sender: A Biosafety Discussion List
From: Curt Speaker
Organization: University Safety - Penn State Univ
Subject: Re: Odor problems - Can you help?
In-Reply-To:
Barb:
Try checking any and all sinks (including cup sinks on lab islands)
to make sure that there is water present in the trap. If the drain
traps are allowed to dry out, and the lab is properly designed (i.e.,
under negative pressure to the outdoors), then what can happen is
that odors from the sewer will find the path of least resistance back
into the building. The negative pressure literally pulls sewer odors
up through the drain lines. Running a few quarts of water down
each and every drain will often solve the problem, and for those
sinks (like cup sinks) that aren't used very often, mineral oil can be
placed in the trap instead of water (it won't evaporate).
It has worked for me here at Penn State.
Hope this helps....
Curt
Curt Speaker
Biosafety Officer
Penn State University
Environmental Health and Safety
speaker@ehs.psu.edu
^...^
(O_O)
=(Y)=
"""
=========================================================================
Date: Mon, 11 Jan 1999 16:34:19 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Odor problems - Can you help?
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Is this a local phenomina? or is it in the whole building?
Local: Look in the areas affected for unused or ignored sinks, uncapped
drain line or very old drain lines. The source would then be the drains
themselves. In unused sinks, the traps dry out and provide the path of
least resistance for the odors and gass to leave the sewer system.
The whole building: Where are the ventilation intakes? If all intakes are
in one place, the source could be your ventilation system supply air. If
you have separate air for each floor or sections. Are the intakes all
together or are they in differnt areas? If only certain areas are affected
and you have multiple ventilation units, check the supply air for the
affected floors or areas.
More information could help.
Hope this helps.
Bob
>Happy New Year.
>
>I am hoping that the collective biosafety community can come up with some
>ideas
>for a problem that is new to our building but apparently not uncommon...
>
>We have a two tank water neutralizing system for the research building. All
>water running from the research laboratory sinks is collected into an 800
>gallon
>tank, mixed, the pipet tips and other solids settle, and the water is gravity
>fed to a second tank for pH adjustment before discharge to POTW. Water from
>animal facilities and service labs in the basements gravity feeds to a
>secondary
>tank system where it sits until the sump pump activates and pumps the water to
>the main tanks.
>
>About 10 months ago we began to experience a nasty odor. There is no
>relationship between odor and use of chemicals for acid/base neutralizing.
>There seems to be a relationship between low water flow and increased odor.
>Engineering has done a great job adjusting ventillation and sealing the
>tanks to
>contain the odor but the building operated without odor problems for years; we
>are concerned about the onset of odors.
>
>Various noses of course react differently. I think that the smell varies
>between something dead and hay infusions. Whatever the odor is, it has
>blackened the copper pipes that run through the area. It can be overwhelming.
>Drain disposal of hazardous, noxious, and a long list of other chemicals is
>forbidden in our location. We have walked the building during early morning
>odor occurances and found no one in the labs
>
>To get to the point, I am wondering if anyone has any experience in similar
>problems. What was the root cause of the odor? Are there any strong
>microbial
>suspects we can or should culture for? If you had this problem, did draining
>and cleaning the tanks end the problem or was that a temporary fix?
>
>Any help or direction will be gratefully accepted and considered.
>
>Hope your 1999 smells better than mine--
>
>Thanks
>Barb Ernisse
>Children's Hospital
>(617)355-6230
>[ernisse_b@a1.tch.harvard.edu}
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Tue, 12 Jan 1999 08:35:01 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Odor problems - Can you help?
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hi Barbara,
I second Judy's response. We had something similar at MIT (a small under
sink neutralizer). The culprits were good old bacteria (a real zoo of
stuff including lots of facultative bacteria, fecal bacteria, anaerobes).
We removed the tank (not needed in that area) and the odor went away.
You may have to take your system offline, drain, scrub and disinfect and
reconnect with oxygen bubbler to rid yourself of the problem.
>Sounds to me like you're doing a little anaerobic biodegradation down
>there. Does it smell like rotten eggs? Hydrogen sulfide from sulfate
>bacteria. Stinky feet? volatile organic chemicals from other microbs.
>Maybe you better stop trying to seal it off and instead bubble some oxygen
>into it. Either that or patent it and sell it to the oil patch.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Tue, 12 Jan 1999 08:51:28 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: BSO -description
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
I saw this request b/f on the newsgroup - but didn't save the responses.
What I need to come up with is a description of the duties,
responsibilities, and authority for a Biological Safety Officer of a
research/hospital/institute. I know what the federal regulations say, I'd
like to know what similar institutes have written down.
If any of you are willing to share your position descriptions for BSO with
me, I'd sure appreciate it. Please send them to me personally.
Thanks,
Judy Pointer
U of TX MD Anderson Cancer Center
Houston, TX 77030
FAX 713 745-1523
TEL 713 745-1423
E-mail jpointer@
=========================================================================
Date: Tue, 12 Jan 1999 08:59:19 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Kyle Boyett
Subject: Re: Odor problems - Can you help?
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Barbara, We had a similar problem in one of our labs a few years ago. The PI
suspected that a sewer like odor was originating from the supply air for the
room. After investigation, my IAQ group determined that the problem was not
in the air supply but rather in an old abandoned p-trap behind some case
work. The trap had run dry obviously, and there was never a cap placed on
the trap when renovations were done years ago. The supply air to the room
created turbulence that gave the illusion that the odor was coming form that
source. Bottom line, check all traps to make sure they are capped if not in
use or filled with water if they are used occasionally. Hope this helps.
Kyle Boyett
Asst. Director of Biosafety
Occupational Health and Safety
University of Alabama at Birmingham
e-mail- kboyett@healthsafe.uab.edu
Phone- 205-934-2487
** Asking me to overlook a safety violation is like asking me to reduce the
value I place on YOUR life**
-----Original Message-----
From: Barbara Ernisse [mailto:ERNISSE_B@A1.TCH.HARVARD.EDU]
Sent: Monday, January 11, 1999 2:55 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Odor problems - Can you help?
Happy New Year.
I am hoping that the collective biosafety community can come up with some
ideas
for a problem that is new to our building but apparently not uncommon...
We have a two tank water neutralizing system for the research building. All
water running from the research laboratory sinks is collected into an 800
gallon
tank, mixed, the pipet tips and other solids settle, and the water is
gravity
fed to a second tank for pH adjustment before discharge to POTW. Water from
animal facilities and service labs in the basements gravity feeds to a
secondary
tank system where it sits until the sump pump activates and pumps the water
to
the main tanks.
About 10 months ago we began to experience a nasty odor. There is no
relationship between odor and use of chemicals for acid/base neutralizing.
There seems to be a relationship between low water flow and increased odor.
Engineering has done a great job adjusting ventillation and sealing the
tanks to
contain the odor but the building operated without odor problems for years;
we
are concerned about the onset of odors.
Various noses of course react differently. I think that the smell varies
between something dead and hay infusions. Whatever the odor is, it has
blackened the copper pipes that run through the area. It can be
overwhelming.
Drain disposal of hazardous, noxious, and a long list of other chemicals is
forbidden in our location. We have walked the building during early morning
odor occurances and found no one in the labs
To get to the point, I am wondering if anyone has any experience in similar
problems. What was the root cause of the odor? Are there any strong
microbial
suspects we can or should culture for? If you had this problem, did
draining
and cleaning the tanks end the problem or was that a temporary fix?
Any help or direction will be gratefully accepted and considered.
Hope your 1999 smells better than mine--
Thanks
Barb Ernisse
Children's Hospital
(617)355-6230
[ernisse_b@a1.tch.harvard.edu}
=========================================================================
Date: Tue, 12 Jan 1999 16:06:44 -0000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stuart Thompson
Subject: Re: BSO -description
In-Reply-To:
MIME-Version: 1.0
Content-Type: multipart/mixed;
boundary="----=_NextPart_000_002C_01BE3E45.8C6509D0"
This is a multi-part message in MIME format.
------=_NextPart_000_002C_01BE3E45.8C6509D0
Content-Type: text/plain;
charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Try this document from the other side of the pond. The law may be different,
but the problems we have to solve and control are probably very similar
Best wishes
Stuart
Dr Stuart Thompson
Health & Safety Services
University of Manchester
Waterloo Place
182/184 Oxford Road
Manchester M13 9PG
tel: +44 (0)161 275 5069
fax: +44 (0)161 275 6989
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Judy Pointer
Sent: 12 January 1999 14:51
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: BSO -description
I saw this request b/f on the newsgroup - but didn't save the responses.
What I need to come up with is a description of the duties,
responsibilities, and authority for a Biological Safety Officer of a
research/hospital/institute.
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////////////////////////////////////////////////////////////////////////////
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////////////////////////////////////////////////////////////////////////////
////////////////////////////////////////////////////////////////////////////
////////////////////////////////////////////////////////////////////////////
////////////////////////////////////////////////////////////////////////////
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------=_NextPart_000_002C_01BE3E45.8C6509D0--
=========================================================================
Date: Tue, 12 Jan 1999 16:10:27 -0000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stuart Thompson
Subject: FW: BSO -description - an apology
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Sorry for inflicting my reply on everyone. I intended to send it only to
Judy Pointer, but hit the wrong key before I had changed the address.
Best wishes
Stuart
Dr Stuart Thompson
Health & Safety Services
University of Manchester
=========================================================================
Date: Tue, 12 Jan 1999 10:34:53 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Separation of trials from research
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
I've got another question for the collective expertise of the group.
I'd like to know what precautions other research/hospital instututes take
for facility separation of clinical materials from basic science research
(non-clinical) materials. This is not initial product production, but
final manipulations prior to patient trial administrations. This specific
trial involves autologous bone marrow cells. We've been able to do most of
these in hospital certified facilities or other dedicted facilities -- but
this one ???? will be difficult to shuffle off that way.
I know the FDA has lots of criteria documents along these lines - so much
so it's overwhelming. I peaked at them a few years back and past out from
exhaustion. I'm not even certain, at this stage, if the IND requires cGMP.
Anyway, if anyone can give me their approach/policy (if standardized) or
refer me to an FDA or other expert in the know, that I could call and
query, I'd appreciate it.
Thanks, in advance
Judy Pointer
jpointer@
=========================================================================
Date: Tue, 12 Jan 1999 09:15:40 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Karen Ruhl
Subject: REMINDER- NEW HIRE SAFETY TRAINING TODAY
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
AT 11:30 IN THE LARGE CONFERENCE ROOM NR1.
For all new hires (including temporary) that have not yet attended a safety
orientation class at CBI.
Questions? Call Karen 5912
=========================================================================
Date: Tue, 12 Jan 1999 12:56:25 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: BSO -description
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Thanks Stuart
=========================================================================
Date: Tue, 12 Jan 1999 13:21:56 EST
Reply-To: Karen=B.=Byers%SS%DFCI@EYE.DFCI.HARVARD.EDU
Sender: A Biosafety Discussion List
From: "Karen B. Byers"
Subject: Re: Separation of trials from research
MIME-Version: 1.0
Content-type: text/plain; charset=us-ascii
Hi Judy.
I had the same question about separation of research and clinical activities
and I also looked for a regulatory answer. I never found it specifically
spelled out in the FDA materials [this was about 8 years ago], although the
previous biosafety officer, Gwladys Caspar, had told me that it was important
to verify compliance on this separation during lab audits. I finally asked
the IBC whether the unwritten policy requiring the separation of research
operations and clinical operations was a policy which I should be enforcing.
Unanimously, the IBC voted to make separation of clinical and research
activities a formal policy. This eliminated questions and simplified my
responsibilities. And the ruling helped the PI get the space required in an
area which could be dedicated to clinical work. Of course, he was initially
offended. And, honestly, I did agree that his lab was very well run. But I
pressed on because I was concerned that allowing clinical/research activities
to go on in the same laboratory would set a precedent which could be cited in
the future, perhaps in less ideal situations. Hope this helps! Good luck!
Karen B. Byers, MS, RBP, CBSP
Biosafety Officer
Dana-Farber Cancer Inst.
44 Binney St.
Boston, MA 02115
karen_byers@dfci.harvard.edu
=========================================================================
Date: Tue, 12 Jan 1999 14:38:06 -0600
Reply-To: "mkinsey@"
Sender: A Biosafety Discussion List
From: Melina Kinsey
Organization: MRI
Subject: Notice: Hemorrhagic Disease, Dogs, FL
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
The following notice I captured from another list group I subscribe to. I
thought it may be of interest to the group.
I have found belonging to the ProMED-mail group has been very informational.
If any of you would like to sign up, I attached the info to the bottom of this
post.
Melina Kinsey
Sr. Biosafey Technician
Midwest Research Institute
mkinsey@
From: ProMED-AHEAD
Date: Tue, 12 Jan 1999 14:00:38 -0500 (EST)
Subject: PRO/AH/EDR> Hemorrhagic disease, dogs - USA (Florida)
HEMORRHAGIC DISEASE, DOGS - USA (FLORIDA)
***********************************************
A ProMED-mail post
Date: Sat, 9 Jan 1999 18:39:50 -0500
From: "George A. Robertson" ,
jlentino , &
Peter Cyrog, DVM
Source: News agency [edited]
Florida -- Derby Lane, the world's oldest continuously operating dog
track, was closed Friday after six greyhounds have died from a mystery
illness and other dogs were ill with a pneumonia-like illness. More
than 100 other dogs, mostly 2- and 3-year-olds, are suffering from
this highly contagious cough. The dogs are coughing up blood and the
pathologist involved is uncertain as to the cause, labeling it a
mystery.
Dr. William Castleman, the University of Florida pathologist who
performed an autopsy on one of the dogs, Gaffer, from the St.
Petersburg race track. "Is it something new?'' Castleman asked. "Is
it a common infection that's just taken a new turn? We don't know.
But the fact [that] this dog became very ill and started bleeding and
died has us thinking there has to be something else going on here.''
Castleman said it will probably take two weeks before scientists can
isolate the pneumonia-like virus.
Trainers reported that dogs suffering the mystery illness were
bleeding through the nose and the mouth, discharging bloody stools.
Some had blood vessels in their skin burst. "Whatever type of
infection this is, it breaks down the clotting mechanisms in the dog.''
The track imposed a quarantine on its greyhounds and set up an
around-the-clock infirmary to combat the illness, which officials say
poses no threat to humans. A necropsy confirmed that one of the dogs
died of a viral respiratory disease and had internal bleeding, said
Sarah Casey, spokeswoman for the University of Florida College of
Veterinary Medicine. [With veterinarians involved, it may not be
_Bordetella_ spp, the common "kennel cough" - Mod.TG]
The dogs at Hollywood Greyhound Track [Florida] have been put under
quarantine after several greyhounds came down with a virus different
than the one in St. Petersburg, Garry Duell, assistant racing
secretary at the track, said Sunday. "It's not the same virus," he
insisted. "It's the normal kennel cough that they get this time of
year.'' Several dogs were scratched from the race card Saturday but
none were taken off Sunday, Duell said.
Dog tracks in Daytona Beach, Jacksonville, Miami, Naples and Orlando
also have quarantined their dogs as a precaution. Races will probably
be scratched at Derby Lane at least through Wednesday, track general
manager Vey Weaver said. Derby Lane is one of the largest taxpaying
pari-mutuels in the state. About 1100 dogs were scheduled to race
there this year. Because of the virus scare, Greyhound Protection
League, a San Francisco-based anti-racing group, has urged owners to
impose a nationwide quarantine at the greyhound tracks.
- --
ProMED-mail
e-mail: promed@usa.
[I expect that we will hear more about this in the future. If any of
our Florida readers know the definitive diagnosis, we would
appreciate you sharing it with us - Mod.TG]
.................................tg/jw
- --
Dr Jack Woodall
Director, ProMED-mail
Institute of Biomedical Sciences
Federal University of Rio de Janeiro
Rio de Janeiro - RJ, Brazil
For all requests, send a message to: "majordomo@usa."
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......................................................................
PROMED-MAIL DIRECTOR, MANAGING EDITOR, MODERATORS AND POLICY COMMITTEE:
JACK WOODALL, Director, ProMED-mail and Chairman, Policy Committee;
Director, Arborvirus Laboratory, New York State Department of Health,
Albany, NY, USA; currently on leave directing the Nucleus for the
Investigation of Emerging Infectious Diseases, Institute of Biomedical
Sciences,
Federal University of Rio de Janeiro, Brazil; formerly Division of
epidemiological surveillance, WHO, Geneva, Switzerland.
ED SCHRODER, Managing Editor, ProMED-mail; National Institute for
Allergy and Infectious Diseases, Div. of Extramural Activities,
Rockville, MD, USA
CHARLES H. CALISHER, ProMED-mail viral diseases Moderator; Professor of
Microbiology, Colorado State University, Fort Collins, CO, USA
MARTIN HUGH-JONES, ProMED-mail animal disease Moderator; Director, WHO
Collaborating Center for Reference and Training in Remote Sensing and
Geographic Information Systems for Veterinary Public Health, Louisiana
State University, Baton Rouge, LA, USA
PETER COWEN, ProMED-mail animal disease Moderator; Director, PAHO/WHO
Consulting Center for Graduate and Residency Training in Veterinary
Public Health, North Carolina State University, Raleigh, NC, USA
KALYAN BANERJEE, ProMED-mail Moderator; former Director, National
Institute of Virology, Pune, India
ALEXANDER VLADYKO, ProMED-mail Moderator; Department of Special
Pathogens, Byelorussian Research Institute of Epidemiology and
Microbiology (BRIEM), Minsk, Belarus
AYOADE M.J. ODUOLA, ProMED-mail Moderator; Department of Pharmacology
and Therapeutics, College of Medicine, University of Ibadan, Ibadan,
Nigeria
PAMELA ANDERSON, Coordinator and Moderator, ProMED-Plant; Centro
Internacional de Agricultura Tropical, Cali, Colombia
DOROTHY B. PRESLAR, Director, ProMED-AHEAD, ProMED-mail Policy
Committee Administrator, Federation of American Scientists, Washington,
DC, USA
STEPHEN S. MORSE, ProMED-mail Policy Committee; Professor, Columbia
University School of Public Health, New York, NY, USA
BARBARA HATCH ROSENBERG, ProMED-mail Policy Committee; Professor, State
University of New York at Purchase, NY, USA
=========================================================================
Date: Wed, 13 Jan 1999 09:40:07 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Odor problems - Can you help?
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Does the system have venting? Could it be blocked? This should not be
happening if the source is the tank. Consult a plumber.
Bob
>Hi Barbara,
>
>I second Judy's response. We had something similar at MIT (a small under
>sink neutralizer). The culprits were good old bacteria (a real zoo of
>stuff including lots of facultative bacteria, fecal bacteria, anaerobes).
>We removed the tank (not needed in that area) and the odor went away.
>
>You may have to take your system offline, drain, scrub and disinfect and
>reconnect with oxygen bubbler to rid yourself of the problem.
>
>
>>Sounds to me like you're doing a little anaerobic biodegradation down
>>there. Does it smell like rotten eggs? Hydrogen sulfide from sulfate
>>bacteria. Stinky feet? volatile organic chemicals from other microbs.
>>Maybe you better stop trying to seal it off and instead bubble some oxygen
>>into it. Either that or patent it and sell it to the oil patch.
>
>Richard Fink, SM(NRM), CBSP
>Assoc. Biosafety Officer
>Mass. Inst. of Tech.
>617-258-5647
>rfink@mit.edu
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Thu, 14 Jan 1999 14:02:33 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Face shield recommendation
What face shields do you recommend to your employees for use as a slash
guard?
Bliss M. Schlank
Biosafety Specialist
Zeneca Pharmaceuticals
1800 Concord Pike
Wilmington DE 19064
302.886.2185
Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Thu, 14 Jan 1999 16:35:59 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: Re: Separation of trials from research
MIME-Version: 1.0
Content-Type: text/plain
Same questions are on my mind. I have downloaded the FDA/CBER guidelines
for Human Somatic Cell Therapy and Gene Therapy, updated 3/98. They are on
the web at:
.
That will be my weekend reading, along with 21 CFR as appropriate. But I
would like to hear what others are doing and how involved they get in FDA as
opposed to NIH guidance issues.
=========================================================================
Date: Fri, 15 Jan 1999 10:19:28 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: Re: Face shield recommendation
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
You may use the face shield of acetate materials.
YK
At 02:02 PM 1/14/99 -0500, you wrote:
>What face shields do you recommend to your employees for use as a slash
>guard?
>Bliss M. Schlank
>Biosafety Specialist
>Zeneca Pharmaceuticals
>1800 Concord Pike
>Wilmington DE 19064
>302.886.2185
>Fax: 302.886.2909
>bliss.schlank@phwilm.
>
>
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
!!!!!!!!
Yu Kwan WAN,
Safety Officer
The Chinese University of Hong Kong
Shatin, NT, Hong Kong
Email:
ulsoykwan@cuhk.edu.hk
ulsoykwan@
ulsoykwan@
ulsoykwan@
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
!!!!!!!!!
=========================================================================
Date: Fri, 15 Jan 1999 13:38:24 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Gill Norton
Organization: University of Western Ontario
Subject: labs for processing human and animal samples
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
As a point of information are there any rules/regulations about whether
animal clinical samples for testing can be handled in the same
laboratory as human clinincal samples? e.g. frozen monkey tissue
samples to be stained and examined in the same laboratory as the
equivalent human tissue slices are being examined for diagnostic work?
Assume that the laboratory personnel would be experienced and using
universal precautions.
--
------------------------------------------------------------------
Gillian Norton
Biosafety Officer
The University of Western Ontario
Occupational Health and Safety
Somerville House, Rm 2345
Phone: (519)661-2036
FAX: (519)661-3420
-------------------------------------------------------------------
=========================================================================
Date: Fri, 15 Jan 1999 12:26:17 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: A Decon Question
MIME-Version: 1.0
Content-Type: text/plain
A scenario for my fellow BIOSAFTY netters -
You have just purchased a new cryofreezer for your BSL3 lab (in which
you study HIV, HTLV, HBV, HCV) and you want to turn your old Revco
chest-type freezer over to Surplus so that someone else can use it. How
would you decon and remove the Revco from the containment lab?
Thanks for sharing your opinions on this. Please respond directly to me
at the e-mail address below. I'll be happy to share the final process
with anyone who wants to know it.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
=========================================================================
Date: Fri, 15 Jan 1999 14:24:51 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Sarah E Palmer
Subject: Laboratory Certification
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Dear Biosafty Members:
Our Institutional Biosafety Committee is about to begin a review to
determine if we should change from the present proposal based tracking
approach to that of laboratory certification. The Committee is
considering a three year certification for exempt and BL1 research and
possibly an annual certification for all BL2 and 3 laboratories.
In order to help the Committee, I want to put together a set of examples
from other IBC's. Those of you that have annual licensing or
certification programs, can you please send me a copy of your form and
time permitting can you let me know what you feel the pros and cons of a
certification program are as opposed to a program that covers every
proposal.
In the interest of space, please email me directly, or fax the information
to me at 520 621-3709.
Thanks in advance for your comments and feedback.
Sincerely,
Sarah Palmer
Sarah Palmer
Institutional Biosafety Committee
PO BOX 210106
Life Sciences South, rm 346
University of Arizona
Tucson AZ 85721-0106
spalmer@U.Arizona.EDU
ph. 520 621-5279
fax 520 621-3709
=========================================================================
Date: Sat, 16 Jan 1999 22:10:47 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Sony SO
Subject: Re: Biohazard sign
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
I would like the standard for the biohazard sign, can I change the
wording of "BIOHAZARD" into "CLINICAL WASTE", or "DANGER OF
INFECTION"....?
Any feedback would be appreciated.
Sony SO
Nursing Officer, Infection Control Team
Princess Margaret Hospital
Email: sonyso@
=========================================================================
Date: Mon, 18 Jan 1999 09:49:15 +1100
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Le Blanc Smith, Peter"
Subject: Re: chemical decontamination of urine/feces
MIME-Version: 1.0
Content-Type: text/plain
I note that the material is collected and presumably smaller quantities
could be taken out of the animal accommodation for your work. Heat treatment
would be better than chemical disinfection. How impractical is sterilization
by autoclave? Do you require sterilization or disinfection?
Chemical treatment may require more processing of the material to ensure
thorough disinfection. You may need to macerate the faeces to ensure any
virus in the faecal mass is more easily contacted by the chosen
disinfectant.
You could consider sodium hydroxide, final concentration of 2% for, say, 24
hours. It would be a cheap and effective disinfectant and is a major
component of "drain cleaners". The alkalinity could be neutralised before
disposal - should there be any environmental concerns with quantities of
strong alkali entering the disposal system.
Just one idea. Hope it helps.
Peter Le Blanc Smith
Biocontainment Microbiologist
Australian Animal Health Laboratory
Private Mail Bag 24
Geelong Vic 3220
Australia
Ph: (03) 5227 5451
Int: +61 3 5227 5451
Fax: (03) 5227 5555
Int: +61 3 5227 5555
E-mail address. Peter.Le.Blanc.Smith@dah.csiro.au
-----Original Message-----
From: Johnson, Julie A. [SMTP:jajohns@IASTATE.EDU]
Sent: Tuesday, January 12, 1999 4:02 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: chemical decontamination of urine/feces
We need to come up with a way to decontaminate collected pig
urine/feces
from pigs infected with a hydrophilic virus. Because of volume and
liquid
state, autoclaving or incineration are not reasonable options. We
would
like to find an appropriate disinfectant that can be added to the
collection
pans. Any suggestions anyone has would be appreciated.
Julie A. Johnson, Biosafety Officer
Iowa State University
Environmental Health & Safety
118 Agronomy Lab
phone: 515-294-7657
fax: 515-294-9357
E-mail: jajohns@iastate.edu
=========================================================================
Date: Mon, 18 Jan 1999 12:16:13 +1300
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stu Macdiarmid
Subject: Re: chemical decontamination of urine/feces -Reply
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: quoted-printable
>>> "Le Blanc Smith, Peter" 18/January/=
1999 11:49am >>>
I note that the material is collected and presumably smaller quantities
could be taken out of the animal accommodation for your work. Heat =
treatment
would be better than chemical disinfection. How impractical is sterilizatio=
n
by autoclave? Do you require sterilization or disinfection?
Chemical treatment may require more processing of the material to ensure
thorough disinfection. You may need to macerate the faeces to ensure any
virus in the faecal mass is more easily contacted by the chosen
disinfectant.
You could consider sodium hydroxide, final concentration of 2% for, say, =
24
hours. It would be a cheap and effective disinfectant and is a major
component of "drain cleaners". The alkalinity could be neutralised before
disposal - should there be any environmental concerns with quantities of
strong alkali entering the disposal system.
Just one idea. Hope it helps.
Peter Le Blanc Smith
Biocontainment Microbiologist
Australian Animal Health Laboratory
Private Mail Bag 24
Geelong Vic 3220
Australia
Ph: (03) 5227 5451
Int: +61 3 5227 5451
Fax: (03) 5227 5555
Int: +61 3 5227 5555
E-mail address. Peter.Le.Blanc.Smith@dah.csiro.au
-----Original Message-----
From: Johnson, Julie A. [SMTP:jajohns@IASTATE.EDU]
Sent: Tuesday, January 12, 1999 4:02 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: chemical decontamination of urine/feces
We need to come up with a way to decontaminate collected pig
urine/feces
from pigs infected with a hydrophilic virus. Because of volume =
and
liquid
state, autoclaving or incineration are not reasonable options. We
would
like to find an appropriate disinfectant that can be added to the
collection
pans. Any suggestions anyone has would be appreciated.
Julie A. Johnson, Biosafety Officer
Iowa State University
Environmental Health & Safety
118 Agronomy Lab
phone: 515-294-7657
fax: 515-294-9357
E-mail: jajohns@iastate.edu
=========================================================================
Date: Mon, 18 Jan 1999 09:59:10 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: Re: Biohazard sign
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Dear Sony
I think you are using this warning sign for disposing clinical waste. You
may design you own word for description. The basic design should include
the biohazard symbol and words "Biohazard" in English and Chinese. The
others just describe the sources or the material.
You may contact the Environmenatal Protection Officer in your area or the
special wastes section. There is a EPD guideline for disposing the wastes.
Actually, I modify it for clinical wastes control in the university campus.
YK Wan
Safety Officer
email: ulsoykwan@cuhk.edu.hk
At 10:10 PM 1/16/99 +0800, you wrote:
>I would like the standard for the biohazard sign, can I change the
>wording of "BIOHAZARD" into "CLINICAL WASTE", or "DANGER OF
>INFECTION"....?
>
>Any feedback would be appreciated.
>
>Sony SO
>Nursing Officer, Infection Control Team
>Princess Margaret Hospital
>Email: sonyso@
>
>
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
!!!!!!!!
Yu Kwan WAN,
Safety Officer
The Chinese University of Hong Kong
Shatin, NT, Hong Kong
Email:
ulsoykwan@cuhk.edu.hk
ulsoykwan@
ulsoykwan@
ulsoykwan@
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
!!!!!!!!!
=========================================================================
Date: Mon, 18 Jan 1999 07:01:42 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Laura Newton
Subject: Re: Biohazard sign
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
No, the word Biohazard with the biohard emblem is specified in the OSHA
Bloodborne Pathogens regulation. Your state may also have specific
requirements for labeling infectious waste that you should be familiar with.
Laura Newton, consultant
-----Original Message-----
From: Sony SO
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Saturday, January 16, 1999 9:14 AM
Subject: Re: Biohazard sign
>I would like the standard for the biohazard sign, can I change the
>wording of "BIOHAZARD" into "CLINICAL WASTE", or "DANGER OF
>INFECTION"....?
>
>Any feedback would be appreciated.
>
>Sony SO
>Nursing Officer, Infection Control Team
>Princess Margaret Hospital
>Email: sonyso@
=========================================================================
Date: Mon, 18 Jan 1999 21:27:23 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Sony SO
Subject: Re: BIOHAZARD SIGN
MIME-Version: 1.0
Content-Type: multipart/mixed; boundary="------------6CCC9A71249"
3o,O$@-S MINE .f&!*: multi-part +H.'.
--------------6CCC9A71249
Content-Type: text/plain; charset=us-ascii
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Thank you for those respone to my inquiry.
According to the OSHA bloodborne standard 29 CFR 1910.1030 The biohazard
sign should be "Labels required by this section shall include the
following legend: [see attached file] (C) These labels shall be
fluorescent orange or orange-red or predominantly so, with lettering or
symbols in a contrasting color."
I want to know do we have other standard or requirement for the
international biohazard sign (except OSHA)?
Thank you.
Sony SO
Nursing Officer, Infection Control Team
Princess Margaret Hospital
Fax: 852-2990-1854
Phone: 852-2990-1861L-Soft list server at MITVMA (1.8c) wrote:
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--------------6CCC9A71249--
=========================================================================
Date: Mon, 18 Jan 1999 09:45:24 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "J.H. Keene"
Subject: Re: Laboratory Certification
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
BE CAREFULL!!! Remember that the NIH Guidelines refer to risks associated
with the gene transfer and ability of an organism to be changed and become
more "hazardous" as a result of that transfer. Biosafety Levels are based
on the probability of certain organisms causing occupationally acquired
infections as a result of working with them in the laboratory. "Exempt
experiments" do not automatically fall into BL-1. Example: Transfer of
E.coli genomic material to a Salmonella is exempt because the transfer
occurs in nature, but it may require BL-2 containment if either the E.
coli(ie. E. coli O157), or the Salmonella (ie. non-debilitated S.
typhimurium) is a potential human pathogen. I would hesitate to reduce the
review with regard to proposals because many researchers do not understand
the differences between the NIH RDNA Guideline requirements and the BMBL
Guidelines for containment. In actuality, you probably should continue
proposal based review and certify your laboratories. Just My Opinion.
John H. Keene, Dr. P.H.
Biohaztec Associates, Inc.
Midlothian, VA
-----Original Message-----
From: Sarah E Palmer
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Friday, January 15, 1999 4:28 PM
Subject: Laboratory Certification
>Dear Biosafty Members:
>
>Our Institutional Biosafety Committee is about to begin a review to
>determine if we should change from the present proposal based tracking
>approach to that of laboratory certification. The Committee is
>considering a three year certification for exempt and BL1 research and
>possibly an annual certification for all BL2 and 3 laboratories.
>
>In order to help the Committee, I want to put together a set of examples
>from other IBC's. Those of you that have annual licensing or
>certification programs, can you please send me a copy of your form and
>time permitting can you let me know what you feel the pros and cons of a
>certification program are as opposed to a program that covers every
>proposal.
>
>In the interest of space, please email me directly, or fax the information
>to me at 520 621-3709.
>
>Thanks in advance for your comments and feedback.
>
>Sincerely,
>Sarah Palmer
>
>Sarah Palmer
>Institutional Biosafety Committee
>PO BOX 210106
>Life Sciences South, rm 346
>University of Arizona
>Tucson AZ 85721-0106
>
>spalmer@U.Arizona.EDU
>ph. 520 621-5279
>fax 520 621-3709
>
=========================================================================
Date: Mon, 18 Jan 1999 11:30:29 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: Laboratory Certification
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Sarah:
If I am having my secretary send you our Colleges Biosafety Manual which
details a well practiced IBC/ Biosafety Committee review format that
includes unannounced site visits to labs doing BSL-1 and 2 registered
work. All BSL-3 level work requires a pre-approval of the lab before
work begins by the IBC/BSC. Hope it helps.
Joe Coggin, Jr. Ph.D. RBP, CBSP
On Fri, 15 Jan 1999, Sarah E Palmer wrote:
> Dear Biosafty Members:
>
> Our Institutional Biosafety Committee is about to begin a review to
> determine if we should change from the present proposal based tracking
> approach to that of laboratory certification. The Committee is
> considering a three year certification for exempt and BL1 research and
> possibly an annual certification for all BL2 and 3 laboratories.
>
> In order to help the Committee, I want to put together a set of examples
> from other IBC's. Those of you that have annual licensing or
> certification programs, can you please send me a copy of your form and
> time permitting can you let me know what you feel the pros and cons of a
> certification program are as opposed to a program that covers every
> proposal.
>
> In the interest of space, please email me directly, or fax the information
> to me at 520 621-3709.
>
> Thanks in advance for your comments and feedback.
>
> Sincerely,
> Sarah Palmer
>
> Sarah Palmer
> Institutional Biosafety Committee
> PO BOX 210106
> Life Sciences South, rm 346
> University of Arizona
> Tucson AZ 85721-0106
>
> spalmer@U.Arizona.EDU
> ph. 520 621-5279
> fax 520 621-3709
>
=========================================================================
Date: Mon, 18 Jan 1999 17:12:14 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Susan Souder
Subject: Serilizing soil
MIME-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: 7bit
Dear Biosafety Members,
I am very interested in finding out if anyone is familiar with
serilizing soil. I was asked the best procedure. I am not certain but
I would probably go the route of dry heat. I realize some spores will
still survive, however, I have not been experienced with this kind of
problem before.
I would appreciate any help. You may reply directly to me.
Thank you
Sue
Susan Souder
Biological Safety Officer
Environmental Health and Safety
Thomas Jefferson University
Phila., Pa. 19107
215-503-7422
FAX 215-503-7727
=========================================================================
Date: Tue, 19 Jan 1999 15:15:09 +1300
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stu Macdiarmid
Subject: Serilizing soil -Reply
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: quoted-printable
Susan Souder asks about procedures for sterilizing soil.=20
I have no advice on soil sterilization but I recall that in the 1960s a =
paper was published in the New Zealand Veterinary Journal dexcribing =
experiments in which the soil in sheep yards was disinfected of Salmonella =
using formalin. I also remember reading of experiments in the United =
Kingdom in which formalin was shown to be useful in disinfecting soil =
contaminated [during wartime bio-warfare experiments] with anthrax.
Stuart C MacDiarmid
National Manager (Agricultural Security)
Regulatory Authority
Ministry of Agriculture and Forestry
PO Box 2526
Wellington
New Zealand.
Phone ;+64-4-474 4100
Fax ;+64-4-474 4133
Email ; macdiarmids@t.nz,
StuartMacDiarmid@
***************************************************************
>>> Susan Souder 19/January/1999 11:12am >>>
Dear Biosafety Members,
I am very interested in finding out if anyone is familiar with
serilizing soil. I was asked the best procedure. I am not certain but
I would probably go the route of dry heat. I realize some spores will
still survive, however, I have not been experienced with this kind of
problem before.
I would appreciate any help. You may reply directly to me.
Thank you
Sue
Susan Souder
Biological Safety Officer
Environmental Health and Safety
Thomas Jefferson University
Phila., Pa. 19107
215-503-7422
FAX 215-503-7727
=========================================================================
Date: Tue, 19 Jan 1999 07:53:46 -0000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stuart Thompson
Subject: Re: Sterilizing soil -Reply
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Stu Macdiarmid
Sent: 19 January 1999 02:15
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Serilizing soil -Reply
Susan Souder asks about procedures for sterilizing soil.
I have no advice on soil sterilization but I recall that in the 1960s a
paper was published in the New Zealand Veterinary Journal dexcribing
experiments in which the soil in sheep yards was disinfected of Salmonella
using formalin. I also remember reading of experiments in the United Kingdom
in which formalin was shown to be useful in disinfecting soil contaminated
[during wartime bio-warfare experiments] with anthrax.
I believe that the name of the island contaminated with anthrax was Gurnard,
so a search of the web may produce details. As I recall, the final
decontamination was achieved only within the last 10 - 20 years, so any
information available should be reasonably up to date. However Britain still
lacks a Freedom of Information Act, so vital facts could still be held
secret by the Ministry of Defence.
Dr Stuart Thompson
Health & Safety Services
University of Manchester
Waterloo Place
182/184 Oxford Road
Manchester M13 9PG
tel: +44 (0)161 275 5069
fax: +44 (0)161 275 6989
=========================================================================
Date: Tue, 19 Jan 1999 09:12:19 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stefan Wagener
Subject: New ABSA Home Page
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hi everyone:
ABSA's has changed their home page!
The new URL is:
This URL will NOT be routed to MSU anymore, since ABSA has switched to a
new ISP (web host). Starting today, the old pages at MSU will be
disconnected. If you have any links to the old page(s), please make sure to
correct the link. Check with the web person in your office and verify the
links on your own home pages. Administration of the new ABSA web site is
now in the hands of David Silberman (davidhs@leland.Stanford.EDU).
Thanks to all of you for your help, encouragement and support. It was a lot
of fun. I will still be involved in web development with a focus on
web-based training (WBT) and safety information technology.
Stefan :-)
Stefan Wagener, PhD, CBSP
Office of Radiation, Chemical and Biological Safety
Michigan State University
Email: stefan@pilot.msu.edu
Phone: (517) 355-6503
Fax: (517) 353-4871
=========================================================================
Date: Tue, 19 Jan 1999 09:39:33 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "J.H. Keene"
Subject: Re: New ABSA Home Page
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Agreat round of applause for Stefan and all the work that went into making
the ABSA homepage what it is today and for encouraging us to move forward
continuing what he has begun. Thanks, Stefan for all your foresight and for
the hours of work you put into that project. Congratulations for a job well
done.
Jack Keene
Biohaztec Associates, Inc.
-----Original Message-----
From: Stefan Wagener
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Tuesday, January 19, 1999 9:13 AM
Subject: New ABSA Home Page
>Hi everyone:
>
>ABSA's has changed their home page!
>
>The new URL is:
>
>
>
>This URL will NOT be routed to MSU anymore, since ABSA has switched to a
>new ISP (web host). Starting today, the old pages at MSU will be
>disconnected. If you have any links to the old page(s), please make sure to
>correct the link. Check with the web person in your office and verify the
>links on your own home pages. Administration of the new ABSA web site is
>now in the hands of David Silberman (davidhs@leland.Stanford.EDU).
>
>Thanks to all of you for your help, encouragement and support. It was a lot
>of fun. I will still be involved in web development with a focus on
>web-based training (WBT) and safety information technology.
>
>
>Stefan :-)
>
>
>
>
>Stefan Wagener, PhD, CBSP
>Office of Radiation, Chemical and Biological Safety
>Michigan State University
>Email: stefan@pilot.msu.edu
>Phone: (517) 355-6503
>Fax: (517) 353-4871
>
=========================================================================
Date: Tue, 19 Jan 1999 09:44:19 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jairo Betancourt
Subject: Re: New ABSA Home Page
MIME-version: 1.0
Content-type: text/plain; charset="iso-8859-1"
Content-transfer-encoding: 7bit
Thank youuu! Stefan. You did a hell of a good job! Kudos to you!
-----Original Message-----
From: Stefan Wagener
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Tuesday, January 19, 1999 9:13 AM
Subject: New ABSA Home Page
>Hi everyone:
>
>ABSA's has changed their home page!
>
>The new URL is:
>
>
>
>This URL will NOT be routed to MSU anymore, since ABSA has switched to a
>new ISP (web host). Starting today, the old pages at MSU will be
>disconnected. If you have any links to the old page(s), please make sure to
>correct the link. Check with the web person in your office and verify the
>links on your own home pages. Administration of the new ABSA web site is
>now in the hands of David Silberman (davidhs@leland.Stanford.EDU).
>
>Thanks to all of you for your help, encouragement and support. It was a lot
>of fun. I will still be involved in web development with a focus on
>web-based training (WBT) and safety information technology.
>
>
>Stefan :-)
>
>
>
>
>Stefan Wagener, PhD, CBSP
>Office of Radiation, Chemical and Biological Safety
>Michigan State University
>Email: stefan@pilot.msu.edu
>Phone: (517) 355-6503
>Fax: (517) 353-4871
=========================================================================
Date: Tue, 19 Jan 1999 10:42:42 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janet Ives
Subject: Autoclaving animal bedding and bioterrorism
In-Reply-To:
MIME-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
Greetings Everyone,
I have recently been presented with two problems which I seek
some advice on.
The first problem is with waste animal bedding from experiments
conducted under ABSL 2 containment. We have some on going
experiments infecting mice with either listeria monocytogenes,
rickettsia rickettsii, or vaccinia virus. Our IBC has recommended
that the soiled animal bedding from ABSL-2 work be autoclaved
prior to being dumped to increase employee safety, but now I am
wondering if this is a case of overkill. Bedding from ABSL 1
experiments are dumped using regularly maintained class 1
dumping stations by personnel wearing N95 respirators, scrubs,
and gloves. All animal bedding is finally disposed of through
incineration on site.
Our Vivarium is experiencing an increase in the number of ABSL 2
experiments would like to know how other academic institutions
are handling their soiled animal bedding. Are you autoclaving? If
you are autoclaving, do you autoclave the cage as an intact unit
that is bagged in the animal room or do you separate the cage
components and then autoclave.
My second problem is more sensitive and if you want to respond
directly to my e-mail, or by fax, or by phone that would be OK. The
country as a whole has experienced many bioterrorism threats.
Our city has had three within the last 3 months. We are in the
process of developing an appropriate response on a University level
as well as on a County/City level. Have any of you wrestled with
the question of how you would handle a such a situation (for
example, a letter indicating that you have been exposed to a
biological agent) if presented at your doorstep? We are very
interested in how people across the country (and abroad) are
dealing with these situations.
Thanks very much.
Janet
Janet Ives, Industrial Hygienist
Department of Environmental Health and Safety
University of Rochester
300 East River Rd. room 23
Rochester, New York 14623
jives@safety.rochester.edu
(716)275-3014 VOICE
(716)256-3155 FAX
=========================================================================
Date: Tue, 19 Jan 1999 10:30:47 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Scott Rusk
Subject: Serilizing soil -Reply
Mime-Version: 1.0
Content-Type: text/plain
I don't have details but you may want to make contact with plant
researchers. I seem to recall that there are systems used for sterilizing
soils for use in greenhouses prior to research projects.
J. Scott Rusk, Director
Environmental Health & Safety
National Animal Disease Center
Ames, IA 50010
phone: 515-239-8258
fax: 515-239-8458
e-mail: srusk@nadc.ars.
>>> Susan Souder 01/18/99 04:12pm
>>>
Dear Biosafety Members,
I am very interested in finding out if anyone is familiar with
serilizing soil. I was asked the best procedure. I am not certain but
I would probably go the route of dry heat. I realize some spores will
still survive, however, I have not been experienced with this kind of
problem before.
I would appreciate any help. You may reply directly to me.
Thank you
Sue
Susan Souder
Biological Safety Officer
Environmental Health and Safety
Thomas Jefferson University
Phila., Pa. 19107
215-503-7422
FAX 215-503-7727
=========================================================================
Date: Tue, 19 Jan 1999 12:20:02 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: Autoclaving animal bedding and bioterrorism
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Sorry, but our protocol for ABSL2 calls for all soiled cages, lids,
bottles, filter lids, bedding and food to be double bagged and
decontaminated before disposal. The cages are bagged in the animal room
and autoclaved "whole", not separated as components.
Hope this is helpful.
At 10:42 AM 1/19/99 -0500, you wrote:
>Greetings Everyone,
>
>I have recently been presented with two problems which I seek
>some advice on.
>
>The first problem is with waste animal bedding from experiments
>conducted under ABSL 2 containment. We have some on going
>experiments infecting mice with either listeria monocytogenes,
>rickettsia rickettsii, or vaccinia virus. Our IBC has recommended
>that the soiled animal bedding from ABSL-2 work be autoclaved
>prior to being dumped to increase employee safety, but now I am
>wondering if this is a case of overkill. Bedding from ABSL 1
>experiments are dumped using regularly maintained class 1
>dumping stations by personnel wearing N95 respirators, scrubs,
>and gloves. All animal bedding is finally disposed of through
>incineration on site.
>
>Our Vivarium is experiencing an increase in the number of ABSL 2
>experiments would like to know how other academic institutions
>are handling their soiled animal bedding. Are you autoclaving? If
>you are autoclaving, do you autoclave the cage as an intact unit
>that is bagged in the animal room or do you separate the cage
>components and then autoclave.
>
>My second problem is more sensitive and if you want to respond
>directly to my e-mail, or by fax, or by phone that would be OK. The
>country as a whole has experienced many bioterrorism threats.
>Our city has had three within the last 3 months. We are in the
>process of developing an appropriate response on a University level
>as well as on a County/City level. Have any of you wrestled with
>the question of how you would handle a such a situation (for
>example, a letter indicating that you have been exposed to a
>biological agent) if presented at your doorstep? We are very
>interested in how people across the country (and abroad) are
>dealing with these situations.
>
>Thanks very much.
>
>Janet
>
>Janet Ives, Industrial Hygienist
>Department of Environmental Health and Safety
>University of Rochester
>300 East River Rd. room 23
>Rochester, New York 14623
>jives@safety.rochester.edu
>(716)275-3014 VOICE
>(716)256-3155 FAX
>
>
Janice Flesher, MS
Principal Industrial Hygienist
Environmental & Occupational Health & Safety Service
University Medical Dental School of New Jersey
97 Paterson Street #227
New Brunswick, NJ 08901
732-235-8497 phone
732-235-8499 fax
=========================================================================
Date: Tue, 19 Jan 1999 12:50:37 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Leonard, Thomas"
Subject: Re: Autoclaving animal bedding and bioterrorism
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Janet,
In my experience, those responsible for cleaning animal cages are often
less than diligent when it comes to properly using dumping
stations--particularly when large numbers of cages are to be cleaned. We
autoclave intact soiled cages prior to dumping.
Regards,
Tom
>The first problem is with waste animal bedding from experiments
>conducted under ABSL 2 containment. We have some on going
>experiments infecting mice with either listeria monocytogenes,
>rickettsia rickettsii, or vaccinia virus. Our IBC has recommended
>that the soiled animal bedding from ABSL-2 work be autoclaved
>prior to being dumped to increase employee safety, but now I am
>wondering if this is a case of overkill. Bedding from ABSL 1
>experiments are dumped using regularly maintained class 1
>dumping stations by personnel wearing N95 respirators, scrubs,
>and gloves. All animal bedding is finally disposed of through
>incineration on site.
>
>Our Vivarium is experiencing an increase in the number of ABSL 2
>experiments would like to know how other academic institutions
>are handling their soiled animal bedding. Are you autoclaving? If
>you are autoclaving, do you autoclave the cage as an intact unit
>that is bagged in the animal room or do you separate the cage
>components and then autoclave.
R. Thomas Leonard, M.S., ASP
Safety Officer
The Wistar Institute
3601 Spruce Street
Philadelphia, PA 19104
tleonard@wistar.upenn.edu
215-898-3712
Fx:215-898-3868
=========================================================================
Date: Tue, 19 Jan 1999 11:30:24 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: Autoclaving animal bedding and bioterrorism
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
I'll try on your first question. At MDA, we do not autoclave cages for
most BL2 organisms b/f dumping BUT we do dump in the biohazard suite in
rooms w/ total exhaust, negative airflow and inside neg pressure HEPA
filtered cage dumpers. The animals (rodents) are all housed in
microisolator cages. The staff wear fit-tested HEPA canaster respirators
and they gown up and garb when entering the biohazard suite, and shower out
at the end of the day and b/f lunch.
The cages and cage racks (sans bedding) are wiped with alcohol or ROH/soap
mixture before they are transported to the centralized cage washer. The
spent bedding, after dumping, is sealed in biohazard boxes and incinerated.
We have autoclaved Mycobaterium avium cages b/f dumping, but have not done
this for Adenovirus cages. We haven't had vaccinia in rodents yet, but
when the time comes I will recommend that the cages be autoclaved b/f
dumping - b/c of the lack of smallpox vaccine, now-a-days, by animal
handlers (either that or make them all get vaccinated - including the
cage/rack wash staff). I'd be more concerned about vacinnia b/c of
possible scratching into the skin, than I am the Adenovirus, which is a
mild disease and most have antibody to already. This is probably splitting
hairs with risk assessment - but it makes me more comfortable. Haven't
worked with the other two agents you mentioned, in vivo - so far.
Judy Pointer
UT MD Anderson
Janet Ives on 01/19/99 09:42:42 AM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: Autoclaving animal bedding and bioterrorism
Greetings Everyone,
I have recently been presented with two problems which I seek
some advice on.
The first problem is with waste animal bedding from experiments
conducted under ABSL 2 containment. We have some on going
experiments infecting mice with either listeria monocytogenes,
rickettsia rickettsii, or vaccinia virus. Our IBC has recommended
that the soiled animal bedding from ABSL-2 work be autoclaved
prior to being dumped to increase employee safety, but now I am
wondering if this is a case of overkill. Bedding from ABSL 1
experiments are dumped using regularly maintained class 1
dumping stations by personnel wearing N95 respirators, scrubs,
and gloves. All animal bedding is finally disposed of through
incineration on site.
Our Vivarium is experiencing an increase in the number of ABSL 2
experiments would like to know how other academic institutions
are handling their soiled animal bedding. Are you autoclaving? If
you are autoclaving, do you autoclave the cage as an intact unit
that is bagged in the animal room or do you separate the cage
components and then autoclave.
My second problem is more sensitive and if you want to respond
directly to my e-mail, or by fax, or by phone that would be OK. The
country as a whole has experienced many bioterrorism threats.
Our city has had three within the last 3 months. We are in the
process of developing an appropriate response on a University level
as well as on a County/City level. Have any of you wrestled with
the question of how you would handle a such a situation (for
example, a letter indicating that you have been exposed to a
biological agent) if presented at your doorstep? We are very
interested in how people across the country (and abroad) are
dealing with these situations.
Thanks very much.
Janet
Janet Ives, Industrial Hygienist
Department of Environmental Health and Safety
University of Rochester
300 East River Rd. room 23
Rochester, New York 14623
jives@safety.rochester.edu
(716)275-3014 VOICE
(716)256-3155 FAX
=========================================================================
Date: Tue, 19 Jan 1999 11:50:44 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Rachael L. Brooks"
Subject: Re: Sterilizing soil
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Hi Susan, Our Vertebrate biology professor keeps a lot of snakes, lizards,
etc. Occasionally we have autoclaved soil for use in the cages. We put it
on the liquid cycle for 20 minutes.
Rachael L. Brooks
Microbiology Lab Coordinator
Texas A&M University - Corpus Christi
=========================================================================
Date: Tue, 19 Jan 1999 12:25:51 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: Sterilizing soil
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Hi Susan
I had to come up with a way to completely sterilize soil for my masters
thesis which involved biodegradation of sorbed Jet fuel out of soil. I had
to seed it with a defined set of microbs - so needed to be sure nothing
else was in it. The only way I could get completely sterilized soil was to
bake it at 450*C for at least 3 hours in aluminim pans. I spread it out no
more than 3 inches thick. I never could get autoclave sterilization to do
the trick - no matter how I packed it.
Judy Pointer.
"Rachael L. Brooks" on 01/19/99 11:50:44 AM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: Re: Sterilizing soil
Hi Susan, Our Vertebrate biology professor keeps a lot of snakes,
lizards,
etc. Occasionally we have autoclaved soil for use in the cages. We put it
on the liquid cycle for 20 minutes.
Rachael L. Brooks
Microbiology Lab Coordinator
Texas A&M University - Corpus Christi
=========================================================================
Date: Tue, 19 Jan 1999 13:34:24 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janet Ives
Subject: Re: Autoclaving animal bedding and bioterrorism
In-Reply-To:
MIME-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
Just a clarification at the request of one of the list members:
I know that Rickettsia rickettsii is considered a biosafety level 3
agent. We follow biosafety level 3 precautions in the laboratory.
Once the agent is in the animal, the containment drops to ABSL 2.
There is no evidence of shedding to the environment from the
animal or of transmission through bites and scratches. That
information comes from one of the world's leaders in rickettsial
research Dr. David Walker.
Thanks.. Janet
Date sent: Tue, 19 Jan 1999 12:20:02 -0500
Send reply to: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: Autoclaving animal bedding and bioterrorism
To: BIOSAFTY@MITVMA.MIT.EDU
Sorry, but our protocol for ABSL2 calls for all soiled cages, lids,
bottles, filter lids, bedding and food to be double bagged and
decontaminated before disposal. The cages are bagged in the animal room
and autoclaved "whole", not separated as components.
Hope this is helpful.
At 10:42 AM 1/19/99 -0500, you wrote:
>Greetings Everyone,
>
>I have recently been presented with two problems which I seek
>some advice on.
>
>The first problem is with waste animal bedding from experiments
>conducted under ABSL 2 containment. We have some on going
>experiments infecting mice with either listeria monocytogenes,
>rickettsia rickettsii, or vaccinia virus. Our IBC has recommended
>that the soiled animal bedding from ABSL-2 work be autoclaved
>prior to being dumped to increase employee safety, but now I am
>wondering if this is a case of overkill. Bedding from ABSL 1
>experiments are dumped using regularly maintained class 1
>dumping stations by personnel wearing N95 respirators, scrubs,
>and gloves. All animal bedding is finally disposed of through
>incineration on site.
>
>Our Vivarium is experiencing an increase in the number of ABSL 2
>experiments would like to know how other academic institutions
>are handling their soiled animal bedding. Are you autoclaving? If
>you are autoclaving, do you autoclave the cage as an intact unit
>that is bagged in the animal room or do you separate the cage
>components and then autoclave.
>
>My second problem is more sensitive and if you want to respond
>directly to my e-mail, or by fax, or by phone that would be OK. The
>country as a whole has experienced many bioterrorism threats.
>Our city has had three within the last 3 months. We are in the
>process of developing an appropriate response on a University level
>as well as on a County/City level. Have any of you wrestled with
>the question of how you would handle a such a situation (for
>example, a letter indicating that you have been exposed to a
>biological agent) if presented at your doorstep? We are very
>interested in how people across the country (and abroad) are
>dealing with these situations.
>
>Thanks very much.
>
>Janet
>
>Janet Ives, Industrial Hygienist
>Department of Environmental Health and Safety
>University of Rochester
>300 East River Rd. room 23
>Rochester, New York 14623
>jives@safety.rochester.edu
>(716)275-3014 VOICE
>(716)256-3155 FAX
>
>
Janice Flesher, MS
Principal Industrial Hygienist
Environmental & Occupational Health & Safety Service
University Medical Dental School of New Jersey
97 Paterson Street #227
New Brunswick, NJ 08901
732-235-8497 phone
732-235-8499 fax
Janet Ives, Industrial Hygienist
Department of Environmental Health and Safety
University of Rochester
300 East River Rd. room 23
Rochester, New York 14623
jives@safety.rochester.edu
(716)275-3014 VOICE
(716)256-3155 FAX
=========================================================================
Date: Tue, 19 Jan 1999 14:46:46 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Gill Norton
Organization: University of Western Ontario
Subject: Re: Autoclaving animal bedding and bioterrorism
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Janet, all our L2 infected animal cages are dumped inside a reverse
horizonal flow HEPA unit adapted for the purpose by having a bin
inserted into the work surface. You can buy these commercially. In
addition the workers should wear N95 respirators and wear separate
gowns gloves shoes etc in the room where the bedding is dumped. If you
try to autoclave the cages intact with the bedding inside the cage and
the cage bagged you will fing that it requires a long time to get a kill
inside the beddding - we did the tests with spore strips in the sawdust
and it took over an hour to get a complete kill ( 121C). Autoclaving
for that long is very hard on the plastic cage material and ruined the
cages after only a few cycles and thats why we went to dumping. Once the
bedding is sealed into bags it can be sprayed with disinfectant, double
bagged and taken to your on-site incinerator. Gillian
Janet Ives wrote:
>
> Greetings Everyone,
>
> I have recently been presented with two problems which I seek
> some advice on.
>
> The first problem is with waste animal bedding from experiments
> conducted under ABSL 2 containment. We have some on going
> experiments infecting mice with either listeria monocytogenes,
> rickettsia rickettsii, or vaccinia virus. Our IBC has recommended
> that the soiled animal bedding from ABSL-2 work be autoclaved
> prior to being dumped to increase employee safety, but now I am
> wondering if this is a case of overkill. Bedding from ABSL 1
> experiments are dumped using regularly maintained class 1
> dumping stations by personnel wearing N95 respirators, scrubs,
> and gloves. All animal bedding is finally disposed of through
> incineration on site.
>
> Our Vivarium is experiencing an increase in the number of ABSL 2
> experiments would like to know how other academic institutions
> are handling their soiled animal bedding. Are you autoclaving? If
> you are autoclaving, do you autoclave the cage as an intact unit
> that is bagged in the animal room or do you separate the cage
> components and then autoclave.
>
> My second problem is more sensitive and if you want to respond
> directly to my e-mail, or by fax, or by phone that would be OK. The
> country as a whole has experienced many bioterrorism threats.
> Our city has had three within the last 3 months. We are in the
> process of developing an appropriate response on a University level
> as well as on a County/City level. Have any of you wrestled with
> the question of how you would handle a such a situation (for
> example, a letter indicating that you have been exposed to a
> biological agent) if presented at your doorstep? We are very
> interested in how people across the country (and abroad) are
> dealing with these situations.
>
> Thanks very much.
>
> Janet
>
> Janet Ives, Industrial Hygienist
> Department of Environmental Health and Safety
> University of Rochester
> 300 East River Rd. room 23
> Rochester, New York 14623
> jives@safety.rochester.edu
> (716)275-3014 VOICE
> (716)256-3155 FAX
--
------------------------------------------------------------------
Gillian Norton
Biosafety Officer
The University of Western Ontario
Occupational Health and Safety
Somerville House, Rm 2345
Phone: (519)661-2036
FAX: (519)661-3420
-------------------------------------------------------------------
=========================================================================
Date: Tue, 19 Jan 1999 16:10:15 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "J.H. Keene"
Subject: Re: Autoclaving animal bedding and bioterrorism
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Be careful when using disinfectant on bedding. 1) It is very difficult to
ensure kill with so much organic material available to use up the
disinfectant. 2) Use of hypochlorite could be dangerous with the ammonia
available in the bedding from urine and feces.
-----Original Message-----
From: Gill Norton
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Tuesday, January 19, 1999 2:48 PM
Subject: Re: Autoclaving animal bedding and bioterrorism
>Janet, all our L2 infected animal cages are dumped inside a reverse
>horizonal flow HEPA unit adapted for the purpose by having a bin
>inserted into the work surface. You can buy these commercially. In
>addition the workers should wear N95 respirators and wear separate
>gowns gloves shoes etc in the room where the bedding is dumped. If you
>try to autoclave the cages intact with the bedding inside the cage and
>the cage bagged you will fing that it requires a long time to get a kill
>inside the beddding - we did the tests with spore strips in the sawdust
>and it took over an hour to get a complete kill ( 121C). Autoclaving
>for that long is very hard on the plastic cage material and ruined the
>cages after only a few cycles and thats why we went to dumping. Once the
>bedding is sealed into bags it can be sprayed with disinfectant, double
>bagged and taken to your on-site incinerator. Gillian
>
>Janet Ives wrote:
>>
>> Greetings Everyone,
>>
>> I have recently been presented with two problems which I seek
>> some advice on.
>>
>> The first problem is with waste animal bedding from experiments
>> conducted under ABSL 2 containment. We have some on going
>> experiments infecting mice with either listeria monocytogenes,
>> rickettsia rickettsii, or vaccinia virus. Our IBC has recommended
>> that the soiled animal bedding from ABSL-2 work be autoclaved
>> prior to being dumped to increase employee safety, but now I am
>> wondering if this is a case of overkill. Bedding from ABSL 1
>> experiments are dumped using regularly maintained class 1
>> dumping stations by personnel wearing N95 respirators, scrubs,
>> and gloves. All animal bedding is finally disposed of through
>> incineration on site.
>>
>> Our Vivarium is experiencing an increase in the number of ABSL 2
>> experiments would like to know how other academic institutions
>> are handling their soiled animal bedding. Are you autoclaving? If
>> you are autoclaving, do you autoclave the cage as an intact unit
>> that is bagged in the animal room or do you separate the cage
>> components and then autoclave.
>>
>> My second problem is more sensitive and if you want to respond
>> directly to my e-mail, or by fax, or by phone that would be OK. The
>> country as a whole has experienced many bioterrorism threats.
>> Our city has had three within the last 3 months. We are in the
>> process of developing an appropriate response on a University level
>> as well as on a County/City level. Have any of you wrestled with
>> the question of how you would handle a such a situation (for
>> example, a letter indicating that you have been exposed to a
>> biological agent) if presented at your doorstep? We are very
>> interested in how people across the country (and abroad) are
>> dealing with these situations.
>>
>> Thanks very much.
>>
>> Janet
>>
>> Janet Ives, Industrial Hygienist
>> Department of Environmental Health and Safety
>> University of Rochester
>> 300 East River Rd. room 23
>> Rochester, New York 14623
>> jives@safety.rochester.edu
>> (716)275-3014 VOICE
>> (716)256-3155 FAX
>
>--
>------------------------------------------------------------------
>Gillian Norton
>Biosafety Officer
>The University of Western Ontario
>Occupational Health and Safety
>Somerville House, Rm 2345
>Phone: (519)661-2036
>FAX: (519)661-3420
>-------------------------------------------------------------------
=========================================================================
Date: Tue, 19 Jan 1999 20:22:29 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Adrian Felts
Subject: Chemical showers and eye wash facilities
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
I'm in the process of reestablishing/reactivating a biosafety program here
at my Institute. I've recently tested the chemical shower and eye wash
fountain and was horrified at the amount of rust that was thrust through
the shower head and eye pieces. Have any of you ever had that problem? Is
it best to replace the equipment? Is this an error of the last biosafey
officer in not testing the shower and fountain periodically?
Thanks ahead of time for any advice and info.
****************************************************************
Adrian Felts
Research Associate and Safety Officer
Anthony G. Gristina Institute for Biomedical Research
=========================================================================
Date: Wed, 20 Jan 1999 10:38:58 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: Re: Chemical showers and eye wash facilities
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
The rust is due to the iron pipe of your water supply. Unless you open the
tape frequently, say every week, the rust will be accumulated in the pipe.
In Hong Kong, our practice is to replace the iron pipe with the copper pipe.
YK Wan
Safety Officer
The Chinese University of Hong Kong
At 08:22 PM 1/19/99 -0500, you wrote:
>I'm in the process of reestablishing/reactivating a biosafety program here
>at my Institute. I've recently tested the chemical shower and eye wash
>fountain and was horrified at the amount of rust that was thrust through
>the shower head and eye pieces. Have any of you ever had that problem? Is
>it best to replace the equipment? Is this an error of the last biosafey
>officer in not testing the shower and fountain periodically?
>
>Thanks ahead of time for any advice and info.
>****************************************************************
>Adrian Felts
>Research Associate and Safety Officer
>Anthony G. Gristina Institute for Biomedical Research
>
>
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
!!!!!!!!
Yu Kwan WAN,
Safety Officer
The Chinese University of Hong Kong
Shatin, NT, Hong Kong
Email:
ulsoykwan@cuhk.edu.hk
ulsoykwan@
ulsoykwan@
ulsoykwan@
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
!!!!!!!!!
=========================================================================
Date: Wed, 20 Jan 1999 09:50:01 -0000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stuart Thompson
Subject: Biological Safety course (CAL)
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Dear Colleagues
I have been asked to recommend a CAL (Computer-assisted learning) course in
biological safety. It should be intelligible from undergraduate level
onwards. Some of you may be thinking that we should design our practicals so
that undergraduates never encounter anything nasty anyway. However the
laudable philosophy behind this course is that making undergraduates (in
particular this target group of pharmacists) aware of safety matters well
before they will face such problems for real is a valid educational
objective.
Can anyone suggest a suitable course?
Best wishes
Stuart
Dr Stuart Thompson
Health & Safety Services
University of Manchester
Waterloo Place
182/184 Oxford Road
Manchester M13 9PG
tel: +44 (0)161 275 5069
fax: +44 (0)161 275 6989
=========================================================================
Date: Wed, 20 Jan 1999 10:41:45 -0000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stuart Thompson
Subject: FW: Disposal of guanidine thiocyanate
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Best wishes
Stuart
Dr Stuart Thompson
Health & Safety Services
University of Manchester
Waterloo Place
182/184 Oxford Road
Manchester M13 9PG
tel: +44 (0)161 275 5069
fax: +44 (0)161 275 6989
-----Original Message-----
From: Stuart Thompson [mailto:Stuart.Thompson@man.ac.uk]
Sent: 15 January 1999 14:55
To: hasnet-link@mailbase.ac.uk
Subject: Disposal of guanidine thiocyanate
One of our molecular biologists uses guanidine thiocyanate (100 - 200g per
series of experiments) as a denaturant for extraction of RNA. Unfortunately,
substitution does not seem to be an option, as the thiocyanate gives much
better results.
He is currently using a disposal company because it is not good practice to
put the residues down the sink. If thiocyanate encounters acid in the
drainage system, cyanide gas can be given off. He would like to avoid this
cost if possible by using an alternative form of disposal (e.g.
inactivation, followed by disposal via the sink)
As usual, safety data sheets on this compound appear to have been written by
lawyers. They recommended diluting the waste with an appropriate solvent and
sending the solution for incineration. My colleague has seen a paper (Boom
et al., J. Clin. Microbiol., 1990, 28, 495-503)which describes collecting
the residues in strong NaOH so that the concentration of alkali never falls
below 0.3M. The fate of this is not described, but he feels that he would
not come to too much harm if he disposed of the alkaline residue in small
batches, say 50 - 100 ml at a time. Would this be ok for a normal
laboratory, with a short run of drains to the main sewer. I would certainly
caution against storing the alkaline solution, as Durant's "Advanced
Inorganic Chemistry" gives the following equation for oxidation of
thiocyanate in alkaline solution:
[SCN]' + 4H2O = [SO4]2' + [CN]' + 8H+ + 6e
Sorry about the formattting, but E-mail does not lend itself to elegant
chemical equations. At least it balances!
Now comes the sting in the tail. My colleague's lab, though populated
largely by University employees, is in the middle of a large hospital, not
far from the Maternity block. That requires extra caution. How do others see
this situation? Am I being overcautious to suggest that his only real option
is to keep paying out to the disposal company?
Best wishes
Stuart
Dr Stuart Thompson
Health & Safety Services
University of Manchester
Waterloo Place
182/184 Oxford Road
Manchester M13 9PG
tel: +44 (0)161 275 5069
fax: +44 (0)161 275 6989
=========================================================================
Date: Wed, 20 Jan 1999 07:03:11 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Philip C. Dugger"
Subject: Re: Chemical showers and eye wash facilities
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; CHARSET=US-ASCII
It's actually very common which is why ANSI recommends weekly
activation of the eyewash; it's also becaus of protozoa that can
accumulate over time. Testing- flushing will prevent an accumulation fo
these undesirables. replacement of the eyewash might be a
consideration but the shower is questionable. I'm not sure it would
matter.
On Tue, 19 Jan 1999 20:22:29 -0500 Adrian Felts
wrote:
>I'm in the process of reestablishing/reactivating a biosafety program
here
>at my Institute. I've recently tested the chemical shower and eye wash
>fountain and was horrified at the amount of rust that was thrust through
>the shower head and eye pieces. Have any of you ever had that
problem? Is
>it best to replace the equipment? Is this an error of the last biosafey
>officer in not testing the shower and fountain periodically?
>
>Thanks ahead of time for any advice and info.
>****************************************************************
>Adrian Felts
>Research Associate and Safety Officer
>Anthony G. Gristina Institute for Biomedical Research
=========================================================================
Date: Wed, 20 Jan 1999 10:34:56 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Education, Continuing"
Subject: Harvard Course Announcement...
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
The Harvard School of Public Health is pleased to offer the following
safety and environmental training courses beginning in March. Please
register as early as possible to guarantee your space. For more
information,
please visit;
=================================
Analyzing Risk: Science, Assessment, and Management
March 2-5, 1999
OSHA's New Respiratory Standard
March 11-12
Comprehensive Industrial Hygiene Review
March 15-19, 1999
Occupational & Environmental Radiation Protection
March 22-26, 1999
Fundamentals of Industrial Hygiene
March 29 - April 2, 1999
Benefit-Cost Analysis for Environmental, Health, and Safety Regulation
April 6-9, 1999
Certified Safety Professional (CSP) Review
April 26-30, 1999
================================
All courses take place at the Harvard School of Public Health, Boston.
================================
For additional information:
Tel: 617-432-1171
Fax: 617-432-1969
E-mail: contedu@sph.harvard.edu
Harvard School of Public Health
Center for Continuing Professional Education
================================
The Harvard School of Public Health is the designated site of a National
Institute for Occupational Safety and Health (NIOSH) Education and
Research Center (ERC) for Occupational Safety and Health. ERC.
=========================================================================
Date: Wed, 20 Jan 1999 10:06:06 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Alfred L. 'Al' Jin"
Subject: Re: Chemical showers and eye wash facilities
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Adrian,
I would like to add to Phil's comments below regarding eyewashes. First,
before you decide to reactivate the eyewash/ shower as part of your
biosafety program, you must first ask yourself a few questions:
(1) Who has ownership of this program. Is it EH&S, Departments, or
Principal Investigator??
(2) Is an eyewash/ shower really needed? OSHA standard 29 CFR1910.151
Paragraph C states, "Where eyes or body of any person may be exposed to
injurious corrosive materials, suitable facilities for quick drenching or
flushing of the eyes or body shall be provided within the work area for
immediate use". If not, you may consider removing the station or taking it
out of service.
(3) Does an eyewash need to be in every laboratory??? Maybe not, in
accordance to the ANSI Z358.1-1990 Standard, it is recommended that
eyewashes be in an accessible location that require no more than 10 seconds
to reach and should be within a (unobstructed) travel distance of 100 feet
from the hazard.
(4) Does every non-ANSI eyewash need to be replaced with ANSI approved
eyewashes???? Check with your IH. If you are the IH, check with EH&S
management as well as PI's Management for extra funding. It's my opinon, a
good compromise would be to replace any new installations with ANSI
approved Units.
(5) What is the frequency for flushing eyewashes and by who??? In
accordance to ANSI Z358.1-1990 Standard (Section 5.5.1), weekly is
recommended. The length of duration for flushing is not mentioned. Please
note that weekly flushing should be documented. I would also recommend
placing a plastic bag over the eyewash pieces to control the splashing of
water. Also check with the Regs for your State. In California, Cal-OSHA
refers to the ANSI Standard. This may differ in your State.
I hope the information is useful (food for thought). In closing, I would
also like to refer you to an article (I'm sure there are more) by Tyndall,
Lyle, and Ironside (Univ. of Tenn. - Zoology Department) entitled, "The
Presence of Free Living Ameobae in Portable and Stationary Eye Wash
Stations", AIHA J 48(11):933-934 (1987). This article talks about the
presence of Acanthamoebae in eyewashes.
Al Jin, BSO, IH, CBSP, MS, CM(AAM), M(ASCP),
Lawrence Livermore National Laboratory, Livermore, CA,
Hazards Control Department, MS-289,
925 423-7385, E-mail:Jin2@
>It's actually very common which is why ANSI recommends weekly
>activation of the eyewash; it's also becaus of protozoa that can
>accumulate over time. Testing- flushing will prevent an accumulation fo
>these undesirables. replacement of the eyewash might be a
>consideration but the shower is questionable. I'm not sure it would
>matter.
>
>
>On Tue, 19 Jan 1999 20:22:29 -0500 Adrian Felts
> wrote:
>>I'm in the process of reestablishing/reactivating a biosafety program
>here
>>at my Institute. I've recently tested the chemical shower and eye wash
>>fountain and was horrified at the amount of rust that was thrust through
>>the shower head and eye pieces. Have any of you ever had that
>problem? Is
>>it best to replace the equipment? Is this an error of the last biosafey
>>officer in not testing the shower and fountain periodically?
>>
>>Thanks ahead of time for any advice and info.
>>****************************************************************
>>Adrian Felts
>>Research Associate and Safety Officer
>>Anthony G. Gristina Institute for Biomedical Research
=========================================================================
Date: Wed, 20 Jan 1999 10:19:57 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: Chemical showers and eye wash facilities
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Adrian -
I'd like to add my two bits. At UCSF, we (EH&S) have the responsibility
for flushing and testing deluge showers and eyewashes. Our local
requirement is for documented flushing of all showers and eyewashes
monthly with volumetric flow testing annually. However, I recommend to
all labs that they activate and flush their eyewashes at least weekly
for at least 10-15 seconds. My concern is based not only on the
Acanthamoeba references Al mentioned, but also on personal experience
(back in my NASA life sciences spaceflight years) with biofilms
containing large amounts of pseudomonads that become firmly embedded in
plumbed water systems. You don't want either of these beasties in your
eyes, especially when there is another irritant present, and regular
frequent flushing will help keep both of these down or (hopefully) out.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Alfred L. 'Al' Jin [mailto:e442242@POPEYE.]
Sent: Wednesday, January 20, 1999 9:06 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: Chemical showers and eye wash facilities
Adrian,
I would like to add to Phil's comments below regarding eyewashes. First,
before you decide to reactivate the eyewash/ shower as part of your
biosafety program, you must first ask yourself a few questions:
(1) Who has ownership of this program. Is it EH&S, Departments, or
Principal Investigator??
(2) Is an eyewash/ shower really needed? OSHA standard 29 CFR1910.151
Paragraph C states, "Where eyes or body of any person may be exposed to
injurious corrosive materials, suitable facilities for quick drenching
or
flushing of the eyes or body shall be provided within the work area for
immediate use". If not, you may consider removing the station or taking
it
out of service.
(3) Does an eyewash need to be in every laboratory??? Maybe not, in
accordance to the ANSI Z358.1-1990 Standard, it is recommended that
eyewashes be in an accessible location that require no more than 10
seconds
to reach and should be within a (unobstructed) travel distance of 100
feet
from the hazard.
(4) Does every non-ANSI eyewash need to be replaced with ANSI approved
eyewashes???? Check with your IH. If you are the IH, check with EH&S
management as well as PI's Management for extra funding. It's my opinon,
a
good compromise would be to replace any new installations with ANSI
approved Units.
(5) What is the frequency for flushing eyewashes and by who??? In
accordance to ANSI Z358.1-1990 Standard (Section 5.5.1), weekly is
recommended. The length of duration for flushing is not mentioned.
Please
note that weekly flushing should be documented. I would also recommend
placing a plastic bag over the eyewash pieces to control the splashing
of
water. Also check with the Regs for your State. In California, Cal-OSHA
refers to the ANSI Standard. This may differ in your State.
I hope the information is useful (food for thought). In closing, I would
also like to refer you to an article (I'm sure there are more) by
Tyndall,
Lyle, and Ironside (Univ. of Tenn. - Zoology Department) entitled, "The
Presence of Free Living Ameobae in Portable and Stationary Eye Wash
Stations", AIHA J 48(11):933-934 (1987). This article talks about the
presence of Acanthamoebae in eyewashes.
Al Jin, BSO, IH, CBSP, MS, CM(AAM), M(ASCP),
Lawrence Livermore National Laboratory, Livermore, CA,
Hazards Control Department, MS-289,
925 423-7385, E-mail:Jin2@
>It's actually very common which is why ANSI recommends weekly
>activation of the eyewash; it's also becaus of protozoa that can
>accumulate over time. Testing- flushing will prevent an accumulation
fo
>these undesirables. replacement of the eyewash might be a
>consideration but the shower is questionable. I'm not sure it would
>matter.
>
>
>On Tue, 19 Jan 1999 20:22:29 -0500 Adrian Felts
> wrote:
>>I'm in the process of reestablishing/reactivating a biosafety program
>here
>>at my Institute. I've recently tested the chemical shower and eye
wash
>>fountain and was horrified at the amount of rust that was thrust
through
>>the shower head and eye pieces. Have any of you ever had that
>problem? Is
>>it best to replace the equipment? Is this an error of the last
biosafey
>>officer in not testing the shower and fountain periodically?
>>
>>Thanks ahead of time for any advice and info.
>>****************************************************************
>>Adrian Felts
>>Research Associate and Safety Officer
>>Anthony G. Gristina Institute for Biomedical Research
=========================================================================
Date: Wed, 20 Jan 1999 14:25:58 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Christina Z Thompson
Subject: Re: Sterilizing soil
MIME-version: 1.0
Content-type: text/plain; charset=us-ascii
My husband used to work in herbicide research. They did a lot of soil
sterilization, as they had to get rid of plant seeds, as well as soil
microorganisms for controlled studies. He says the only way to completely
sterilize soil is to autoclave it. Dry heat does not do the job.
Keep in mind, 'sterilizing' soil (it isn't really sterile) for greenhouse use
is really a different animal than sterilizing to kill some potential pathogen
that you don't want to release into the environment. So if they found that
dry heat does not work for greenhouse use, then it surely wouldn't work to
kill pathogens you don't want to spread around in the environment.
And however you load it, you'd have to do validation runs to prove you're
getting to all the soil in the middle of the load.
Chris Thompson
Biosafety Officer
Eli Lilly and Company
317-277-4795
=========================================================================
Date: Wed, 20 Jan 1999 14:55:37 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Melissa Nellis
Subject: Re: ...and bioterrorism
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hi Janet,
We are also looking at the question of how to respond to the threat
of bioterrorism. The National Symposium on Medical and Public Health
Response to Bioterrorism is being held in Arlington, VA on Feb 16-17. The
symposium is presented by Johns Hopkins Center for Civilian Biodefense
Studies, US Dept of Health and Human Services, Infectious Disease Society
of America, and the American Society for Microbiology. The program looks
good and I am hoping to get some good information that will help us address
our concerns. Registration is $395. Phone number for questions is
1-800-481-7901. I can FAX you a copy of the program if you are interested.
Melissa
>My second problem is more sensitive and if you want to respond
>directly to my e-mail, or by fax, or by phone that would be OK. The
>country as a whole has experienced many bioterrorism threats.
>Our city has had three within the last 3 months. We are in the
>process of developing an appropriate response on a University level
>as well as on a County/City level. Have any of you wrestled with
>the question of how you would handle a such a situation (for
>example, a letter indicating that you have been exposed to a
>biological agent) if presented at your doorstep? We are very
>interested in how people across the country (and abroad) are
>dealing with these situations.
>Janet Ives, Industrial Hygienist
>Department of Environmental Health and Safety
>University of Rochester
>300 East River Rd. room 23
>Rochester, New York 14623
>jives@safety.rochester.edu
>(716)275-3014 VOICE
>(716)256-3155 FAX
_______________________________________________________________
Melissa A. Nellis, MPH
Research Biosafety Specialist Phone:(612)626-5892
University of Minnesota FAX: (612)624-1949
Environmental Health and Safety nelli001@maroon.tc.umn.edu
Boynton Health Service W-140
410 Church St. SE
Minneapolis, Minnesota 55455 Website: dehs.umn.edu
_______________________________________________________________
=========================================================================
Date: Wed, 20 Jan 1999 17:25:22 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Andy Garcia-Rivera
Subject: Re: ...and bioterrorism
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hi Melissa,
Happy New Year
Can you send me a fax with the info on the bioterrorism conference.
Hope all is well with you!
My fax is 607-255-8267 --Andy
At 02:55 PM 1/20/99 -0500, you wrote:
>Hi Janet,
> We are also looking at the question of how to respond to the threat
>of bioterrorism. The National Symposium on Medical and Public Health
>Response to Bioterrorism is being held in Arlington, VA on Feb 16-17. The
>symposium is presented by Johns Hopkins Center for Civilian Biodefense
>Studies, US Dept of Health and Human Services, Infectious Disease Society
>of America, and the American Society for Microbiology. The program looks
>good and I am hoping to get some good information that will help us address
>our concerns. Registration is $395. Phone number for questions is
>1-800-481-7901. I can FAX you a copy of the program if you are interested.
>
>Melissa
>
>>My second problem is more sensitive and if you want to respond
>>directly to my e-mail, or by fax, or by phone that would be OK. The
>>country as a whole has experienced many bioterrorism threats.
>>Our city has had three within the last 3 months. We are in the
>>process of developing an appropriate response on a University level
>>as well as on a County/City level. Have any of you wrestled with
>>the question of how you would handle a such a situation (for
>>example, a letter indicating that you have been exposed to a
>>biological agent) if presented at your doorstep? We are very
>>interested in how people across the country (and abroad) are
>>dealing with these situations.
>>Janet Ives, Industrial Hygienist
>>Department of Environmental Health and Safety
>>University of Rochester
>>300 East River Rd. room 23
>>Rochester, New York 14623
>>jives@safety.rochester.edu
>>(716)275-3014 VOICE
>>(716)256-3155 FAX
>
>
>
>_______________________________________________________________
> Melissa A. Nellis, MPH
> Research Biosafety Specialist Phone:(612)626-5892
> University of Minnesota FAX: (612)624-1949
> Environmental Health and Safety nelli001@maroon.tc.umn.edu
> Boynton Health Service W-140
> 410 Church St. SE
> Minneapolis, Minnesota 55455 Website: dehs.umn.edu
>_______________________________________________________________
>
>
=========================================================================
Date: Wed, 20 Jan 1999 13:25:27 -1000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Hubert B Olipares
Subject: Soil Sterilization
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
In the state of Hawaii we have a very restrictive import requirement for
soil. State Dept of Agriculture requires the following for sterilization
of soil
All soil sample and residues shall be treated by autoclaving at
15 PSI for 30 mins or longer and shall not exceed two inch in depth. For
dry heat the following schedule is recommended:
110.0 - 120.5 C (230-249F) 16 hours exposure period
121.0 - 154.0 C (250-209F) 2 hours
154.5 - 192.5 C (310-379F) 30 minutes
193.0 - 220.0 C (380-429F) 4 minutes
221.0 - 232.0 C (430-450F) 2 minutes
All water resides must also be treated.
+++++++++++++++++++++++++++++++++
+Hubert B. Olipares, RBP +
+Biological Safety Officer +
+University of Hawaii +
+2040 East-West Road +
+Honolulu, Hawaii 96822-2022 +
+ +
+Telephone: 808-956-3197 +
+Fax: 808-956-3205 +
+E-Mail: olipares@hawaii.edu +
+++++++++++++++++++++++++++++++++
=========================================================================
Date: Thu, 21 Jan 1999 12:21:18 +0100
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: HAHNS
Subject: Xeontransplant Regulation & Biosafet concerns
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hi,
I would like to have other's opinion about possible biosafety regulation
about xenotransplant.
Specially, if anyone knows about individual country's regulation, I would
like to know about it.
Thank you very much in advance.
Sincerely,
S. Hahn
Brussels, Belgium
=========================================================================
Date: Thu, 21 Jan 1999 09:20:41 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Petuch, Brian R."
Subject: Re: Sterilizing soil
MIME-version: 1.0
Content-type: text/plain
Content-transfer-encoding: 7BIT
While I was at Ayerst Labs, we used sandy loam to store actinomycete spores.
The soil was moistened with water and autoclaved 3 times, with storage at
room temp. in between. We did not experience any contamination.
> ----------
> From: Christina Z Thompson[SMTP:THOMPSON_CHRISTINA_Z@]
> Sent: Wednesday, January 20, 1999 2:25 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Re: Sterilizing soil
>
> My husband used to work in herbicide research. They did a lot of soil
> sterilization, as they had to get rid of plant seeds, as well as soil
> microorganisms for controlled studies. He says the only way to completely
> sterilize soil is to autoclave it. Dry heat does not do the job.
>
> Keep in mind, 'sterilizing' soil (it isn't really sterile) for greenhouse
> use
> is really a different animal than sterilizing to kill some potential
> pathogen
> that you don't want to release into the environment. So if they found
> that
> dry heat does not work for greenhouse use, then it surely wouldn't work to
> kill pathogens you don't want to spread around in the environment.
>
> And however you load it, you'd have to do validation runs to prove you're
> getting to all the soil in the middle of the load.
>
> Chris Thompson
> Biosafety Officer
> Eli Lilly and Company
> 317-277-4795
>
=========================================================================
Date: Thu, 21 Jan 1999 11:03:50 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: ML Graham
Subject: Re: Xenotransplantation Regulations
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
From: Mary Louise Graham on 01/21/99 11:03 AM
With respect to Canadian guidelines etc., I believe we are only at the
draft stage so far. The following are sites in Health Canada where you can
find several xenotransplantation documents/reports which may be of
interest.
(1) Excerpts from the Report of the National Forum on Xenotransplantation
Clinical, Ethical and Regulatory Issues:
(2) Report of the National Forum on Xenotransplantation - Clinical, Ethical
and Regulatory Issues
(3) Synopsis of seminar XENOTRANSPLANTATION: BIOLOGICAL AND ETHICAL
ISSUES, Speaker: Fritz H. Bach, M.D., Harvard Medical School, November 24,
1998
Hope this is of assistance,
Mary Louise Graham
Chief, Biocontainment and Certification
Office of Biosafety
Laboratory Centre for Disease Control
Health Canada
HAHNS @ on 99/01/21 06:21:18 AM
Please respond to BIOSAFTY@MITVMA.MIT.EDU
To: BIOSAFTY @ MITVMA.MIT.EDU
cc: (bcc: Mary Louise Graham)
Subject: Xeontransplant Regulation & Biosafet concerns
Hi,
I would like to have other's opinion about possible biosafety regulation
about xenotransplant.
Specially, if anyone knows about individual country's regulation, I would
like to know about it.
Thank you very much in advance.
Sincerely,
S. Hahn
Brussels, Belgium
=========================================================================
Date: Thu, 21 Jan 1999 15:24:49 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janet Ives
Subject: Re: ...and bioterrorism
In-Reply-To:
MIME-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
Melissa,
Thanks for the information. Yes please fax the program to me. My
fax number is 716-256-3155.
Thanks again.
Janet
Date sent: Wed, 20 Jan 1999 14:55:37 -0500
Send reply to: A Biosafety Discussion List
From: Melissa Nellis
Subject: Re: ...and bioterrorism
To: BIOSAFTY@MITVMA.MIT.EDU
Hi Janet,
We are also looking at the question of how to respond to the threat
of bioterrorism. The National Symposium on Medical and Public Health
Response to Bioterrorism is being held in Arlington, VA on Feb 16-17. The
symposium is presented by Johns Hopkins Center for Civilian Biodefense
Studies, US Dept of Health and Human Services, Infectious Disease Society
of America, and the American Society for Microbiology. The program looks
good and I am hoping to get some good information that will help us address
our concerns. Registration is $395. Phone number for questions is
1-800-481-7901. I can FAX you a copy of the program if you are interested.
Melissa
>My second problem is more sensitive and if you want to respond
>directly to my e-mail, or by fax, or by phone that would be OK. The
>country as a whole has experienced many bioterrorism threats.
>Our city has had three within the last 3 months. We are in the
>process of developing an appropriate response on a University level
>as well as on a County/City level. Have any of you wrestled with
>the question of how you would handle a such a situation (for
>example, a letter indicating that you have been exposed to a
>biological agent) if presented at your doorstep? We are very
>interested in how people across the country (and abroad) are
>dealing with these situations.
>Janet Ives, Industrial Hygienist
>Department of Environmental Health and Safety
>University of Rochester
>300 East River Rd. room 23
>Rochester, New York 14623
>jives@safety.rochester.edu
>(716)275-3014 VOICE
>(716)256-3155 FAX
_______________________________________________________________
Melissa A. Nellis, MPH
Research Biosafety Specialist Phone:(612)626-5892
University of Minnesota FAX: (612)624-1949
Environmental Health and Safety nelli001@maroon.tc.umn.edu
Boynton Health Service W-140
410 Church St. SE
Minneapolis, Minnesota 55455 Website: dehs.umn.edu
_______________________________________________________________
Janet Ives, Industrial Hygienist
Department of Environmental Health and Safety
University of Rochester
300 East River Rd. room 23
Rochester, New York 14623
jives@safety.rochester.edu
(716)275-3014 VOICE
(716)256-3155 FAX
=========================================================================
Date: Thu, 21 Jan 1999 15:28:01 -0500
Reply-To: eagleson@
Sender: A Biosafety Discussion List
From: Eagleson
Subject: Bioterrorism & Gene Delivery Technologies
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
The conversation about bioterrorism prompted me to announce the 1999
ABSA/Eagleson Spring Seminar Series, which includes a course on
Bioterrorism.
This year's event will take place on May 5-8 in Atlanta. (Atlanta was the #1
choice on the survey I sent out in the fall.) Emory University is our host.
We are offering the following two courses simultaneously on Thursday and
Friday:
*Emergency Response to Bioterrorism with Stefan Wagener, Ph.D. and Robert
Hawley, Ph.D.
This two-day course is specifically designed to train and
inform health and safety professionals, emergence responders and law
enforcement personnel about the real risk associated with biological agents.
The course will not only cover the most likely biological agents, but also
their acquisition and delivery, legislation, emergency response,
decontamination and protection, as well as biosecurity.
*Biological Safety Issues in the Development and Use of Recombinant DNA
Gene Delivery Technologies with Tom Kost, Ph.D.
and Patrick Condreay, Ph.D.
This two-day course begins with an overview of recombinant DNA technology
and virus replication processes. This is followed by more specialized topics
of gene delivery technologies, with an emphasis on viral vectors and their
application in the research laboratory, animal facilities, and gene therapy.
In addition, Wednesday tours will take us to Yerkes Primate Center,
including the brand new HIV Vaccine Research Center, the Emory campus, and
the CDC Odyssey museum. Tours will be limited to the first forty
registrants.
A new addition this year is a Saturday morning class, an Update on TB and
Hepatitis, facilitated by Jonathan Richmond.
Brochures will be available in two weeks. If you would like to be one of the
first to receive the Spring Seminar brochure, please email us at
eagleson@.
Please feel free to call me if you have any questions.
Mary Ann Sondrini
Eagleson Institute
P.O. Box 954
Sanford, ME 04073
(207) 490-1076
(207) 324-3869 -Fax
=========================================================================
Date: Fri, 22 Jan 1999 10:00:32 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: Biosafety level 3 laboratory
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Do any one know the resources on how to build biosafety level 3 laboratory?
There is little information from the association of architect or engineers
in Hong Kong.
YK Wan
Safety Officer
The Chinese University of Hong Kong
email: ulsoykwan@cuhk.edu.hk
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
!!!!!!!!
Yu Kwan WAN,
Safety Officer
The Chinese University of Hong Kong
Shatin, NT, Hong Kong
Email:
ulsoykwan@cuhk.edu.hk
ulsoykwan@
ulsoykwan@
ulsoykwan@
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
!!!!!!!!!
=========================================================================
Date: Fri, 22 Jan 1999 11:02:54 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: Procedure on BSC Certification
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
In Hong Kong, most engineers do the biosafety cabinets performance test
according to the British Standard Institution. I am trained in Eagleson
Institute in USA and examined under the Field Certifier Accreditation
Program of NSF.
The engineer take the 100% reference directly from the aerosol generator and
scan the HEPA filter at range of 100 instead of 0.01. The scanning rate is
same as the NSF. As he was trained at the manufacturer in UK, I cannot
determine whether it is the standard technique in UK.
Can anyone familarize the procedures in UK? I can be reached at email
"ulsoykwan@cuhk.edu.hk".
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
!!!!!!!!
Yu Kwan WAN,
Safety Officer
The Chinese University of Hong Kong
Shatin, NT, Hong Kong
Email:
ulsoykwan@cuhk.edu.hk
ulsoykwan@
ulsoykwan@
ulsoykwan@
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
!!!!!!!!!
=========================================================================
Date: Fri, 22 Jan 1999 09:08:55 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Leslie Delpin
Subject: Re: Biosafety level 3 laboratory
In-Reply-To:
Yu Wan,
The American Public Health Association (APHA) has a publication
"Designing a Modern Microbiological/Biomedical Laboratory- Lab Design
Process and Technology" which came out in 1997 that you may find
useful. Also you may want to contact Paul Langevin with Agriculture
Canada (613-759-6936). He is an engineer who has presented BL3 lab
design courses at ABSA.
Leslie Delpin - Biological Health and Safety Manager
University of Connecticut
Environmental Health and Safety
3102 Horsebarn Hill Rd. Box U-97
Storrs, CT 06269-4097
(860)486-3613
(860)486-1106
lmdelpin@safety.ehs.uconn.edu
=========================================================================
Date: Fri, 22 Jan 1999 09:41:57 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Mike Pirrello
Subject: Re: Biosafety level 3 laboratory
MIME-Version: 1.0
Content-Type: text/plain
It appears that the document you mention is available from APHA's
Fax-On-Demand service by calling (703) 531-0894 and selecting publication
number 621.
Michael G. Pirrello, CHMM (BSO)
Safety & Environmental Mgr.
Trimeris, Inc.
Mpirrello@
-----Original Message-----
From: Leslie Delpin [SMTP:lmdelpin@SAFETY.EHS.UCONN.EDU]
Subject: Re: Biosafety level 3 laboratory
The American Public Health Association (APHA) has a publication
"Designing a Modern Microbiological/Biomedical Laboratory- Lab
Design
Process and Technology" which came out in 1997 that you may find
useful.
=========================================================================
Date: Fri, 22 Jan 1999 13:58:50 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: CREM Biosafety Course
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
FYI:
>
>Please note the following information concerning the 1999 edition of
>Biosafety in the Laboratory: Principles and Practices to be held in
>Winnipeg, Manitoba, Canada.
>
>Our web site is located at:
>
>Microbio/bmi/biohaz/biohazcoursehome.htm
>
>With Regards,
>
>Dr. Syed A. Sattar, Ph.D.
>Director, CREM
>
>-------------------
>BIOSAFETY IN THE LABORATORY: PRINCIPLES AND PRACTICES
>JUNE 10-11, 1999
>FEDERAL LABORATORIES FOR HEALTH CANADA
>1015 ARLINGTON ST., WINNIPEG, MANITOBA, CANADA
>SPONSORED BY CREM
>
>
>
>
>--
>Jason Tetro, B.Sc.
>Executive Assistant, Centre for Research on Environmental Microbiology
>University of Ottawa
>jtetro@cyberus.ca
>Phone: (613) 562-5800 x 8314
>Address: Room 4119, 451 Smyth Rd.
> University of Ottawa
> Ottawa, Ontario, Canada
> K1H 8M5
>
>
>
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
=========================================================================
Date: Fri, 22 Jan 1999 18:03:45 EST
Reply-To: Karen=B.=Byers%SS%DFCI@EYE.DFCI.HARVARD.EDU
Sender: A Biosafety Discussion List
From: "Karen B. Byers"
Subject: Re: Biosafety level 3 laboratory
MIME-Version: 1.0
Content-type: text/plain; charset=us-ascii
The NIH Design Policy and Guidelines are also very helpful for Biosafety
Level 3 design criteria. These documents are at
Information on Level 3 is found under
"research laboratory" as well as "vivarium" for animal work. Hope this helps!
=========================================================================
Date: Sun, 24 Jan 1999 19:49:12 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Disinfection/inactivation of tissue culture media
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
I'm looking for some assistance with respect to inactivation of tissue culture
media.
At present, the lab has a vacuum system which collects waste material from
biological safety cabinets into a vacuum bottle containing a sodium
hypochlorite (1/4 of the bottle). The waste material is discarded to the sewer
system. There is a concern from the local water pollution control authority
regarding the minute quantities of mercury in the bleach. They would prefer
bleach not be used. However, they have not provided acceptable alternatives.
Autoclaving cannot be performed at this time due to space limitations. It is
not clear to me what viruses they are actually dealing with.
Any other suggestions for inactivation?
Regards,
Edward Krisiunas, MT(ASCP), CIC, MPH
Consultant
Spectrum
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
ekrisiunas@
=========================================================================
Date: Sun, 24 Jan 1999 22:07:33 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "J.H. Keene"
Subject: Re: Disinfection/inactivation of tissue culture media
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Is there really mercury in hypochlorite? Am I the only one that has not
heard about mercury in hypochlorite?
-----Original Message-----
From: Ed Krisiunas
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Sunday, January 24, 1999 7:51 PM
Subject: Disinfection/inactivation of tissue culture media
>I'm looking for some assistance with respect to inactivation of tissue
culture
>media.
>
>At present, the lab has a vacuum system which collects waste material from
>biological safety cabinets into a vacuum bottle containing a sodium
>hypochlorite (1/4 of the bottle). The waste material is discarded to the
sewer
>system. There is a concern from the local water pollution control authority
>regarding the minute quantities of mercury in the bleach. They would prefer
>bleach not be used. However, they have not provided acceptable
alternatives.
>Autoclaving cannot be performed at this time due to space limitations. It
is
>not clear to me what viruses they are actually dealing with.
>
>Any other suggestions for inactivation?
>
>Regards,
>
>Edward Krisiunas, MT(ASCP), CIC, MPH
>Consultant
>Spectrum
>115 Lyons Road
>Burlington, Connecticut
>06013
>860-675-1217
>860-675-1311(fax)
>ekrisiunas@
=========================================================================
Date: Sun, 24 Jan 1999 22:45:56 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Erik Talley
Subject: Re: Disinfection/inactivation of tissue culture media
MIME-Version: 1.0
Content-Type: text/plain
Hmmm. No Hg in NaOCl. Are we talking parts per septillion?
However, depending on what it had been used for I could see mercury
contamination in the vacuum pump line which would end up in the waste
sodium hypochlorite solution.
Erik
___________________________________
Erik A. Talley, Assistant Director
Center for Environmental Health and Safety
Southern Illinois University Carbondale
erik@cehs.siu.edu
-----Original Message-----
From: J.H. Keene [mailto:jkeene@]
Sent: Sunday, January 24, 1999 9:08 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: Disinfection/inactivation of tissue culture media
Is there really mercury in hypochlorite? Am I the only one that has not
heard about mercury in hypochlorite?
-----Original Message-----
From: Ed Krisiunas
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Sunday, January 24, 1999 7:51 PM
Subject: Disinfection/inactivation of tissue culture media
>I'm looking for some assistance with respect to inactivation of tissue
culture
>media.
>
>At present, the lab has a vacuum system which collects waste material
from
>biological safety cabinets into a vacuum bottle containing a sodium
>hypochlorite (1/4 of the bottle). The waste material is discarded to
the
sewer
>system. There is a concern from the local water pollution control
authority
>regarding the minute quantities of mercury in the bleach. They would
prefer
>bleach not be used. However, they have not provided acceptable
alternatives.
>Autoclaving cannot be performed at this time due to space limitations.
It
is
>not clear to me what viruses they are actually dealing with.
>
>Any other suggestions for inactivation?
>
>Regards,
>
>Edward Krisiunas, MT(ASCP), CIC, MPH
>Consultant
>Spectrum
>115 Lyons Road
>Burlington, Connecticut
>06013
>860-675-1217
>860-675-1311(fax)
>ekrisiunas@
=========================================================================
Date: Mon, 25 Jan 1999 05:58:29 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Re: Disinfection and inactivation
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
Good morning to all:
Jack/Erik: I'm in agreement with your comments. This is a new one on me as
well.
One response I received directly regarding my question on disinfection and
inactivation of tissue culture media. I to had not heard of mercury in bleach
until I recently read an article related to the Memorandum of understanding
between the USEPA and the American Hospital Association regarding elimination
of mercury from the health care environment and waste minimization. I believe
the issue of mercury in bleach solutions (or at least the concern) is isolated
to water pollution control authorities in select cities.
"That seems to be a common dilema where the environmental and biosafety
fields meet. I suggest that you analyze the bleach brands for mercury
content until you find a brand that is non-detect for mercury. Then pass
that information along to the water pollution people in the hope that they
let you continue using bleach. I think the problem isn't using bleach, it
is concern over bleach that is poorly made with high levels of mercury.
Perhaps they will agree to let you use the bleach if it is tested to be
clean for mercury.
Many institutions have tested the bleach used for disinfecting tissue
culture media in order to ensure that they are doing all they can to
minimize mercury discharge in the building
Edward Krisiunas, MT(ASCP), CIC, MPH
Consultant
Spectrum
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
ekrisiunas@
=========================================================================
Date: Mon, 25 Jan 1999 09:28:24 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: bleach and Hg
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
According to the Mass. Water Resources Authority:
"The compiled Database provides the most current and complete information
available on the
sources of mercury present within the products consumed by the Member
Hospitals and
Institutions. Using all available inputs, a total of 5,504 products have
been identified and
inventoried. A significant number of these products have also been tested
and found to contain
mercury at some level. In addition, 118 common products, such as bleach,
alcohol, laboratory
lids and embedded tissue samples, have been identified as significant
sources of mercury that
were unknowingly being discharged to the sewerage system. "
Apparently they found Hg in some brands of household bleaches. Hg is
soluble in Sodium hypochlorite solutions so perhaps in manufacturing, some
Hg contamination slips in.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Mon, 25 Jan 1999 09:38:26 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Barbara Ernisse
Subject: Re: bleach and Hg
In-Reply-To:
MIME-version: 1.0
Content-type: TEXT/PLAIN; CHARSET=US-ASCII
Content-transfer-encoding: 7BIT
Unfortunately for us, the level of mercury we can send out in our waste water
is restricted to less than 2 ppb. No, that is not a typographical error. The
Massachusetts Water Resources Authority, (MWRA, the POTW for eastern MA) set
this restriction to eliminate mercury from the sludge they market as fertilizer.
Achieving and maintaining this level can be challenging. As Richie quoted, one
of the unexpected sources of mercury is household bleach which can have Hg
contamination from the manufacturing process. We have tested many brands of
bleach to find a supply that is both low in Hg and registered with the EPA as a
hospital disinfectant. And we still ship out bleach solutions from some labs as
chemical waste.
It's always something
Barb Ernisse
Children's Hospital
Boston
=========================================================================
Date: Mon, 25 Jan 1999 10:05:08 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "J.H. Keene"
Subject: Re: bleach and Hg
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Once again our reach for zero risk gets us into trouble. When will we
realize that there is no such thing as zero risk and proceed with reason?
Jack Keene
Biohaztec Associates
-----Original Message-----
From: Richard Fink
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Monday, January 25, 1999 9:25 AM
Subject: bleach and Hg
>According to the Mass. Water Resources Authority:
>
>"The compiled Database provides the most current and complete information
>available on the
>sources of mercury present within the products consumed by the Member
>Hospitals and
>Institutions. Using all available inputs, a total of 5,504 products have
>been identified and
>inventoried. A significant number of these products have also been tested
>and found to contain
>mercury at some level. In addition, 118 common products, such as bleach,
>alcohol, laboratory
>lids and embedded tissue samples, have been identified as significant
>sources of mercury that
>were unknowingly being discharged to the sewerage system. "
>
>Apparently they found Hg in some brands of household bleaches. Hg is
>soluble in Sodium hypochlorite solutions so perhaps in manufacturing, some
>Hg contamination slips in.
>
>
>
>Richard Fink, SM(NRM), CBSP
>Assoc. Biosafety Officer
>Mass. Inst. of Tech.
>617-258-5647
>rfink@mit.edu
>
=========================================================================
Date: Mon, 25 Jan 1999 10:07:05 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Barbara Ernisse
Subject: Re: bleach and Hg
In-Reply-To:
MIME-version: 1.0
Content-type: TEXT/PLAIN; CHARSET=US-ASCII
Content-transfer-encoding: 7BIT
Sorry about the obvious omission, I am having a very Monday morning:
We found that Chlorox and Star bleach (Star is manufactured by Austin) have
mercury levels of 20 ppb or less and EPA registration. Crown bleach is also
at the same mercury levels but has no registration on the label (at last check).
Asking the manufacturers for mercury information is not helpful for us, the
level we are concerned with is considered mercury free for manufacturing,
shipping and recording purposes.
This information may not be all inclusive, there may very well be a number of
other products out there that we have not tested.
Barb
=========================================================================
Date: Mon, 25 Jan 1999 12:07:02 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Jean.Goldberg"
Subject: Prions from Mice
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; CHARSET=US-ASCII
One of our researchers just called me with the following
concern. One of his colleagues is investigating prions
from mice. He would like to run cells infected with the
prions through a cell sorter. The cell sorter is located
on an open bench. The researcher who called me is
concerned that this work might generate aerosols that are
hazardous to people working in the lab. His colleague
insists that there is absolutely no hazard, and that he
handles the prions with no gloves or other controls at
another institution. I would appreciate your thoughts and
comments on this. Thanks in advance.
----------------------------------------
Jean Goldberg
Email: Jean.Goldberg@Med.Nyu.Edu
"NYU Medical Center"
=========================================================================
Date: Mon, 25 Jan 1999 12:53:49 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Prions from Mice
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
At 12:07 PM 1/25/99 -0500, you wrote:
>One of our researchers just called me with the following
>concern. One of his colleagues is investigating prions
>from mice.
>
>----------------------------------------
>Jean Goldberg
>Email: Jean.Goldberg@Med.Nyu.Edu
>"NYU Medical Center"
>
I would err on the side of caution. It has been shown that prions can
cross specie "barrier" though typically it takes a longer incubation time
to get disease when it crosses. While no mouse prion has been shown to
cause disease in humans, is that because it can't or because research with
the agent has been for less time then the required incubation time? This
is basically a researcher betting their life that it can't cross. Is it
infectious via an aerosol - unknown; what is the infectious dose - unknown.
Can it infect via ingestion (remembering that some of what is inhaled will
be ingested) - probably based on the mad cow disease in the U.K. How long
does the prion remain infectious - years. With all of this I would insist
on a minimum of BL2 with strict aerosol control to prevent surfaces
becoming contaminated as decontamination of lab surfaces is very problematic.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Mon, 25 Jan 1999 11:45:42 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Alfred L. 'Al' Jin"
Subject: Re: Prions from Mice
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/enriched; charset="us-ascii"
Jean,
A few years ago, I had the opportunity to review and give comments (as
a favor) on the topic of cell sorters. The paper was sponsored by the
International Society of Analytical Cytology (ISAC) Biohazard Working
Group. The fruit of everyone's labor now appears in Cytometry
28:99-117 (1997) and is entitled, "Biosafety Guidelines for
Sorting of Unfixed Cells" by Ingrid Schmid, etal. (who works at the
Dept of Hematology and Oncology at the University of California at Los
Angeles).
Enclosed is the abstract from the paper:
"The International Society of Analytical Cytology (ISAC) Biohazard
Working Group present guidelines for sorting of unfixed cell, including
known biohazardous samples, using jet-in-air, deflected droplet cell
sorters. There is a risk that personnel operating these instruments
could become exposed to droplet and aerosols containing biological
agents present in the sample. The following guidelines can aid in the
prevention of exposures of laboratory personnel to pathogens contained
in the sort samples. The document provides biosafety recommendations
for sample handling, operator training and protection, laboratory
facility design and instrument set-up and maintenance. In addition it
describes in detail methods for assessment of instrument aerosol
containment. Recommendations provided here may also help laboratories
to obtain institutional and/or regulatory agency approval for sorting
of unfixed and known biohazardous samples."
In summary, all aspects of biosafety are discussed in this paper, but
the bottom line is the cell sorter itself. Most (newer) cell sorters
provide for a negative pressure environment in which the cells are
counted. This negative pressure environment will provide adequate
protection to the users if the stream is undisturbed. The problem is
created when the stream is distrupted or if the room ventilation is not
balanced. In addition to the biosafety issues involved, don't forget
that most of these units involve the use of lasers, so one must also
focus (excuse the pun) on the laser hazard.
In closing, I hope this helps.
Al Jin, BSO, IH, CBSP, MS, CM(AAM), M(ASCP),
Lawrence Livermore National Laboratory, Livermore, CA,
Hazards Control Department, MS-289,
925 423-7385, E-mail:Jin2@
>One of our researchers just called me with the following
>concern. One of his colleagues is investigating prions
>from mice. He would like to run cells infected with the
>prions through a cell sorter. The cell sorter is located
>on an open bench. The researcher who called me is
>concerned that this work might generate aerosols that are
>hazardous to people working in the lab. His colleague
>insists that there is absolutely no hazard, and that he
>handles the prions with no gloves or other controls at
>another institution. I would appreciate your thoughts and
>comments on this. Thanks in advance.
>
>----------------------------------------
>Jean Goldberg
>Email: Jean.Goldberg@Med.Nyu.Edu
>"NYU Medical Center"
=========================================================================
Date: Mon, 25 Jan 1999 17:13:16 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "J.H. Keene"
Subject: Re: Prions from Mice
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
When in doubt, as we seem to be in this case, contain the process and keep
people from being exposed. The investigator that says there is no risk is
probably related to those lab personnel who used to say that "normal serum"
is harmless and blood is sterile unless it comes from someone with a known
infection. Those folks had a one in four chance of developing Hepatitis B.
As Rich says, we don't know about the infectivity of these prions in humans
and we should be careful with them.
-----Original Message-----
From: Jean.Goldberg
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Monday, January 25, 1999 12:17 PM
Subject: Prions from Mice
>One of our researchers just called me with the following
>concern. One of his colleagues is investigating prions
>from mice. He would like to run cells infected with the
>prions through a cell sorter. The cell sorter is located
>on an open bench. The researcher who called me is
>concerned that this work might generate aerosols that are
>hazardous to people working in the lab. His colleague
>insists that there is absolutely no hazard, and that he
>handles the prions with no gloves or other controls at
>another institution. I would appreciate your thoughts and
>comments on this. Thanks in advance.
>
>----------------------------------------
>Jean Goldberg
>Email: Jean.Goldberg@Med.Nyu.Edu
>"NYU Medical Center"
=========================================================================
Date: Mon, 25 Jan 1999 16:55:54 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Daniel King
Subject: Re: Biosafety level 3 laboratory
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
United States Department of Agriculture Standards for the different
biosafety levels are given in Manual 242.1-ARS - ARS Facilities Design
Standards as well as several other manuals unrelated to construction are
available at two websites - (with
search capability) and (without
search capability).
Daniel J. King
USDA, ARS, SE Poultry Research Lab
934 College Station Rd.
Athens, GA 30605 USA
706-546-3407
706-546-3161 FAX
dking@asrr.
On Fri, 22 Jan 1999, Wan Yu Kwan wrote:
> Do any one know the resources on how to build biosafety level 3 laboratory?
> There is little information from the association of architect or engineers
> in Hong Kong.
>
> YK Wan
> Safety Officer
> The Chinese University of Hong Kong
> email: ulsoykwan@cuhk.edu.hk
> !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
> !!!!!!!!
>
> Yu Kwan WAN,
> Safety Officer
> The Chinese University of Hong Kong
> Shatin, NT, Hong Kong
>
> Email:
> ulsoykwan@cuhk.edu.hk
> ulsoykwan@
> ulsoykwan@
> ulsoykwan@
>
> !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
> !!!!!!!!!
>
=========================================================================
Date: Mon, 25 Jan 1999 13:11:06 -1000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Hubert B Olipares
Subject: Re: (Fwd) (Fwd) bleach and Hg (fwd)
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
---------- Forwarded message ----------
To: SAFETY@LIST.UVM.EDU
Subject: Re: (Fwd) (Fwd) bleach and Hg
Bleach is manufactured from dilute sodium hyroxide and chlorine gas. There
are no points in the manufacturing process were Mercury could be introduced.
HOWEVER, some grades of bleach are manufactured from a "low-salt" grade of
sodium hydroxide, in which the membrane used in the manufacturing process
is composed OF mercury (Mercury-cell/Rayon Grade). Sodium hydroxide from
this manufacturing process typically has a MAXIMUM of 0.2 ppm mercury
content. Therfore, most industrial strength bleach (12.5%) would have a
mercury content of 0.060 ppm at worst.
Household bleach is 5.25% by weight.
The Chlor-Alkalai industry recognizes that even this amount of mercury
needs to be reduced. It has prepared a report to the EPA which can be found
at: .
The Western Lake Superior Sanitary District (Duluth, MN) has found more
mercury in waste generated by Dental Clinics, than can be attributed to
industrial or house-hold bleach.
=========================================================================
Date: Mon, 25 Jan 1999 16:07:45 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: Prions from Mice
MIME-Version: 1.0
Content-Type: text/plain
Hi, Jean --
At UCSF, we consider all animal-origin prions (scrapie, transmissible
mink encephalopathy) to be Risk Group 2 and all human-origin prions
(CJD, GSS, FFI, etc.) as well as BSE ("mad cow" prion, thought to be
associated with the so-called "New Variant CJD" in Europe) to be Risk
Group 3. Thus, tissue cultures inoculated with CNS material from a CJD
patient, and transgenic mice carrying a human prion transgene are both
handled under BSL3 containment. The "colleague" who handles
prion-contaminated material completely without controls needs a good
talking-to by his BSO.
There is no documented evidence of aerosol transmission of prion
infectivity. Under natural conditions, prion illnesses are spread by
ingestion. But as Richie implied, we've only just begun to really start
learning about this agent and its natural history, and to make rash
assumptions about its harmlessness is foolhardy at least and may be
fatal at best.
In my opinion, the cell sorter should be run under minimum BSL2
containment, with proper controls in use during all operations, and
decontamination done with 1N NaOH or fresh undiluted bleach following
the run. Resultant purified or concentrated materials should be
considered infectious at the RG2 level if the prion is one of animal
origin other than BSE, or RG3 if of human origin.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Jean.Goldberg [mailto:Jean.Goldberg@MED.NYU.EDU]
Sent: Monday, January 25, 1999 9:07 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Prions from Mice
One of our researchers just called me with the following
concern. One of his colleagues is investigating prions
from mice. He would like to run cells infected with the
prions through a cell sorter. The cell sorter is located
on an open bench. The researcher who called me is
concerned that this work might generate aerosols that are
hazardous to people working in the lab. His colleague
insists that there is absolutely no hazard, and that he
handles the prions with no gloves or other controls at
another institution. I would appreciate your thoughts and
comments on this. Thanks in advance.
----------------------------------------
Jean Goldberg
Email: Jean.Goldberg@Med.Nyu.Edu
"NYU Medical Center"
=========================================================================
Date: Tue, 26 Jan 1999 10:00:08 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Janet S. Peterson"
Subject: security policy
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
To members of the Biosafty List,
The University of Maryland is in the process of developing a
security policy for research facilities on campus that will encompass
both new construction and retrofit where necessary. The policy will be
limited to research facilities. There will probably be recommendations
for several levels of security, depending on the level of risk in
specific types of laboratories.
Do any of you have security policies for your research
facilities? If you do, would you be willing to share them with me? As
we draft our policy it would be helpful to have examples of formal
policies from other institutions. Thank you for your help. Janet
******************************************
Janet S. Peterson, CBSP
Biological Safety Officer
University of Maryland
Department of Environmental Safety
7505 Yale Avenue
College Park, MD 20742
Tel: 301/405-3975; Fax: 301/314-9294
******************************************
=========================================================================
Date: Tue, 26 Jan 1999 13:35:48 PST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: teresa stimpfel
Subject: Help Please - Disinfectant
Mime-Version: 1.0
Content-Type: text/plain
Hi All,
Problem I need the name of a commerical cleaning product that the
manufacturer agress will kill hepatitis and staph and is presuemable is
registered as a disinfectant. Please respond directly to me at
terystim@
Background- A sanitary line backed up into a grease interceptor and
floor drains in a cafeteria on site. Found and severed the plumbing
connection which did not appear on any building plans. Contractor
immediately acted and had all floors traps etc. cleaned by a reputable
company using a product called Milgro by DriEaz. Then the contractor
contacted the municpal health inspector to reopen the cafeteria.
Health inspector stated correctly that Dri Eaz does not ceritify this
product to kill hepatitis but will certify for staph which the inspector
is concerned about. It is more than a WEEK since cleaning and time alone
(lost by the usual finger pointing and poor communication)is resolving
any residual helath issues.
The inspector rightly refuses to reopen. However he will NOT accept
1:10 bleach and will not provide the name of any acceptable product that
is "certified" (his words) for hepatitis. I presume he means a product
that is registered as a viricial disinfectant that the mfgr will state
kills hep A.
I need the name of a product that is "certifed" for hepatitis (inspector
is not distinguishing among the viruses) to staisfy the municpal health
inspector. We need to get this cafe reopened. Of course I'm away from
my office. Thanks again for any help you can give.
Terry Stimpfel - Montclair State Univ
Terry
______________________________________________________
Get Your Private, Free Email at
=========================================================================
Date: Tue, 26 Jan 1999 17:28:15 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Petuch, Brian R."
Subject: Re: Help Please - Disinfectant
MIME-version: 1.0
Content-type: text/plain
Content-transfer-encoding: 7BIT
You might find answers at the following URL:
> ----------
> From: teresa stimpfel[SMTP:terystim@]
> Sent: Tuesday, January 26, 1999 4:35 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Help Please - Disinfectant
>
> Hi All,
> Problem I need the name of a commerical cleaning product that the
> manufacturer agress will kill hepatitis and staph and is presuemable is
> registered as a disinfectant. Please respond directly to me at
> terystim@
>
> Background- A sanitary line backed up into a grease interceptor and
> floor drains in a cafeteria on site. Found and severed the plumbing
> connection which did not appear on any building plans. Contractor
> immediately acted and had all floors traps etc. cleaned by a reputable
> company using a product called Milgro by DriEaz. Then the contractor
> contacted the municpal health inspector to reopen the cafeteria.
>
> Health inspector stated correctly that Dri Eaz does not ceritify this
> product to kill hepatitis but will certify for staph which the inspector
> is concerned about. It is more than a WEEK since cleaning and time alone
> (lost by the usual finger pointing and poor communication)is resolving
> any residual helath issues.
>
> The inspector rightly refuses to reopen. However he will NOT accept
> 1:10 bleach and will not provide the name of any acceptable product that
> is "certified" (his words) for hepatitis. I presume he means a product
> that is registered as a viricial disinfectant that the mfgr will state
> kills hep A.
>
> I need the name of a product that is "certifed" for hepatitis (inspector
> is not distinguishing among the viruses) to staisfy the municpal health
> inspector. We need to get this cafe reopened. Of course I'm away from
> my office. Thanks again for any help you can give.
> Terry Stimpfel - Montclair State Univ
>
> Terry
>
> ______________________________________________________
> Get Your Private, Free Email at
>
=========================================================================
Date: Tue, 26 Jan 1999 17:16:27 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: Re: Help Please - Disinfectant
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I copied this directly off of the NAIN list of EPA registered products at
this web site:
National Antimicrobial Information Network
NAIN operates as a cooperative agreement between Oregon State University and
the US Environmental Protection Agency.
NAIN can be reached at 800-447-6349 Monday-Friday from 7:30-4:30 pacific
time.
List D: Products registered with the EPA which are effective against
Hepatitis B Virus and HIV.
This information is provided as a service of the National Antimicrobial
Information Network and is based on the most recent information available
from the EPA as of October, 1998. This list is updated periodically as new
information is obtained, accounting for label changes, cancellations,
transferals of registrations, and label edits. It is a violation of Federal
Law to use a pesticide in a manner inconsistent
with the label. The label is the law!
Product: ACTRIL COLD STERILANT
EPA Reg: 52252-7
Manuf: MINNTECH CORP, RENAL SYSTEMS, DIV OF MINNTECH CORP.
Form: SOLUTION-READY-TO-USE
Active Ingredients (%):
HYDROGEN PEROXIDE 00.8000%
PEROXYACETIC ACID 00.0600%
Product: BARQUAT 42Z-10
EPA Reg: 6836-57
Manuf: LONZA INC
Form: SOLUBLE-CONCENTRATE
Active Ingredients (%):
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 5.000%
ALKYL DIMETHYL ETHYLBENZYL AMMONIUM CHLORIDE 5.000%
Product: D125
EPA Reg: 61178-1
Manuf: MICROGEN, INC.
Form: SOLUBLE-CONCENTRATE
Active Ingredients (%):
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 02.2500%
ALKYL DIMETHYL ETHYLBENZYL AMMONIUM CHLORIDE 02.2500%
Product: D125 AG-10
EPA Reg: 46519-1
Manuf: PRINCE BEAUTY AND INFECTION CONTROL, LLC
Form: SOLUBLE-CONCENTRATE
Active Ingredients (%):
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 5.000%
ALKYL DIMETHYL ETHYLBENZYL AMMONIUM CHLORIDE 5.000%
Product: MINNCARE COLD STERILANT
EPA Reg: 52252-4
Manuf: MINNTECH CORP, RENAL SYSTEMS, DIV OF MINNTECH CORP.
Form: SOLUBLE-CONCENTRATE
Active Ingredients (%):
HYDROGEN PEROXIDE 22.000%
PEROXYACETIC ACID 04.500%
Product: PUBLIC PLACES
EPA Reg: 61178-2
Manuf: MICROGEN, INC.
Form: SOLUTION-READY-TO-USE
Active Ingredients (%):
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 00.0700%
ALKYL DIMETHYL ETHYLBENZYL AMMONIUM CHLORIDE 00.0700%
Product: PUBLIC PLACES TOWELETTE
EPA Reg: 61178-4
Manuf: MICROGEN, INC.
Form: IMPREGNATED-MATERIALS
Active Ingredients (%):
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 00.0700%
ALKYL DIMETHYL ETHYLBENZYL AMMONIUM CHLORIDE 00.0700%
Product: STAT-5 ANTIMICROBIAL SPRAY
EPA Reg: 69264-1
Manuf: MCCORMICK DISTILLING CO INC.
Form: SOLUTION-READY-TO-USE
Active Ingredients (%):
ETHANOL 65.8700%
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 00.0900%
NOTE: If you have questions regarding a particular products registration
status, you can compare its EPA registration number with the EPA
Reg: number on this list. The EPA Reg: number may consist of two or three
sets of digits separated by hyphens "-". The first number refers to
the Registrants Identification number. The second number represents the
Product identification number. The third number represents the
Distributor/Relabeler Identification number. This lists contains the primary
registrants products. Other companies may repackage and rename
products but do not change the formulation. These renamed products do not
appear on this list. Some products may have more than one trade
name for a registration number.
-----Original Message-----
From: teresa stimpfel [mailto:terystim@]
Sent: Tuesday, January 26, 1999 3:36 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Help Please - Disinfectant
Hi All,
Problem I need the name of a commerical cleaning product that the
manufacturer agress will kill hepatitis and staph and is presuemable is
registered as a disinfectant. Please respond directly to me at
terystim@
Background- A sanitary line backed up into a grease interceptor and
floor drains in a cafeteria on site. Found and severed the plumbing
connection which did not appear on any building plans. Contractor
immediately acted and had all floors traps etc. cleaned by a reputable
company using a product called Milgro by DriEaz. Then the contractor
contacted the municpal health inspector to reopen the cafeteria.
Health inspector stated correctly that Dri Eaz does not ceritify this
product to kill hepatitis but will certify for staph which the inspector
is concerned about. It is more than a WEEK since cleaning and time alone
(lost by the usual finger pointing and poor communication)is resolving
any residual helath issues.
The inspector rightly refuses to reopen. However he will NOT accept
1:10 bleach and will not provide the name of any acceptable product that
is "certified" (his words) for hepatitis. I presume he means a product
that is registered as a viricial disinfectant that the mfgr will state
kills hep A.
I need the name of a product that is "certifed" for hepatitis (inspector
is not distinguishing among the viruses) to staisfy the municpal health
inspector. We need to get this cafe reopened. Of course I'm away from
my office. Thanks again for any help you can give.
Terry Stimpfel - Montclair State Univ
Terry
______________________________________________________
Get Your Private, Free Email at
=========================================================================
Date: Wed, 27 Jan 1999 10:22:27 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Petuch, Brian R."
Subject: Re: Help Please - Disinfectant
MIME-version: 1.0
Content-type: text/plain; charset=iso-8859-1
Content-transfer-encoding: 7BIT
Just a caution with peracetic acid-containing disinfectants; you could get
involved with exposure to acetic acid.
> ----------
> From: Johnson, Julie A.[SMTP:jajohns@IASTATE.EDU]
> Sent: Tuesday, January 26, 1999 6:16 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Re: Help Please - Disinfectant
>
> I copied this directly off of the NAIN list of EPA registered products at
> this web site:
>
> National Antimicrobial Information Network
> NAIN operates as a cooperative agreement between Oregon State University
> and
> the US Environmental Protection Agency.
> NAIN can be reached at 800-447-6349 Monday-Friday from 7:30-4:30 pacific
> time.
>
>
> List D: Products registered with the EPA which are effective against
> Hepatitis B Virus and HIV.
>
> This information is provided as a service of the National Antimicrobial
> Information Network and is based on the most recent information available
> from the EPA as of October, 1998. This list is updated periodically as new
> information is obtained, accounting for label changes, cancellations,
> transferals of registrations, and label edits. It is a violation of
> Federal
> Law to use a pesticide in a manner inconsistent
> with the label. The label is the law!
>
> Product: ACTRIL COLD STERILANT
> EPA Reg: 52252-7
> Manuf: MINNTECH CORP, RENAL SYSTEMS, DIV OF MINNTECH CORP.
> Form: SOLUTION-READY-TO-USE
> Active Ingredients (%):
> HYDROGEN PEROXIDE 00.8000%
> PEROXYACETIC ACID 00.0600%
>
> Product: BARQUAT 42Z-10
> EPA Reg: 6836-57
> Manuf: LONZA INC
> Form: SOLUBLE-CONCENTRATE
> Active Ingredients (%):
> ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 5.000%
> ALKYL DIMETHYL ETHYLBENZYL AMMONIUM CHLORIDE 5.000%
>
> Product: D125
> EPA Reg: 61178-1
> Manuf: MICROGEN, INC.
> Form: SOLUBLE-CONCENTRATE
> Active Ingredients (%):
> ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 02.2500%
> ALKYL DIMETHYL ETHYLBENZYL AMMONIUM CHLORIDE 02.2500%
>
> Product: D125 AG-10
> EPA Reg: 46519-1
> Manuf: PRINCE BEAUTY AND INFECTION CONTROL, LLC
> Form: SOLUBLE-CONCENTRATE
> Active Ingredients (%):
> ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 5.000%
> ALKYL DIMETHYL ETHYLBENZYL AMMONIUM CHLORIDE 5.000%
>
> Product: MINNCARE COLD STERILANT
> EPA Reg: 52252-4
> Manuf: MINNTECH CORP, RENAL SYSTEMS, DIV OF MINNTECH CORP.
> Form: SOLUBLE-CONCENTRATE
> Active Ingredients (%):
> HYDROGEN PEROXIDE 22.000%
> PEROXYACETIC ACID 04.500%
>
> Product: PUBLIC PLACES
> EPA Reg: 61178-2
> Manuf: MICROGEN, INC.
> Form: SOLUTION-READY-TO-USE
> Active Ingredients (%):
> ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 00.0700%
> ALKYL DIMETHYL ETHYLBENZYL AMMONIUM CHLORIDE 00.0700%
>
> Product: PUBLIC PLACES TOWELETTE
> EPA Reg: 61178-4
> Manuf: MICROGEN, INC.
> Form: IMPREGNATED-MATERIALS
> Active Ingredients (%):
> ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 00.0700%
> ALKYL DIMETHYL ETHYLBENZYL AMMONIUM CHLORIDE 00.0700%
>
> Product: STAT-5 ANTIMICROBIAL SPRAY
> EPA Reg: 69264-1
> Manuf: MCCORMICK DISTILLING CO INC.
> Form: SOLUTION-READY-TO-USE
> Active Ingredients (%):
> ETHANOL 65.8700%
> ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 00.0900%
>
> NOTE: If you have questions regarding a particular products registration
> status, you can compare its EPA registration number with the EPA
> Reg: number on this list. The EPA Reg: number may consist of two or three
> sets of digits separated by hyphens "-". The first number refers to
> the Registrants Identification number. The second number represents the
> Product identification number. The third number represents the
> Distributor/Relabeler Identification number. This lists contains the
> primary
> registrants products. Other companies may repackage and rename
> products but do not change the formulation. These renamed products do not
> appear on this list. Some products may have more than one trade
> name for a registration number.
>
> -----Original Message-----
> From: teresa stimpfel [mailto:terystim@]
> Sent: Tuesday, January 26, 1999 3:36 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Help Please - Disinfectant
>
>
> Hi All,
> Problem I need the name of a commerical cleaning product that the
> manufacturer agress will kill hepatitis and staph and is presuemable is
> registered as a disinfectant. Please respond directly to me at
> terystim@
>
> Background- A sanitary line backed up into a grease interceptor and
> floor drains in a cafeteria on site. Found and severed the plumbing
> connection which did not appear on any building plans. Contractor
> immediately acted and had all floors traps etc. cleaned by a reputable
> company using a product called Milgro by DriEaz. Then the contractor
> contacted the municpal health inspector to reopen the cafeteria.
>
> Health inspector stated correctly that Dri Eaz does not ceritify this
> product to kill hepatitis but will certify for staph which the inspector
> is concerned about. It is more than a WEEK since cleaning and time alone
> (lost by the usual finger pointing and poor communication)is resolving
> any residual helath issues.
>
> The inspector rightly refuses to reopen. However he will NOT accept
> 1:10 bleach and will not provide the name of any acceptable product that
> is "certified" (his words) for hepatitis. I presume he means a product
> that is registered as a viricial disinfectant that the mfgr will state
> kills hep A.
>
> I need the name of a product that is "certifed" for hepatitis (inspector
> is not distinguishing among the viruses) to staisfy the municpal health
> inspector. We need to get this cafe reopened. Of course I'm away from
> my office. Thanks again for any help you can give.
> Terry Stimpfel - Montclair State Univ
>
> Terry
>
> ______________________________________________________
> Get Your Private, Free Email at
>
=========================================================================
Date: Wed, 27 Jan 1999 11:22:16 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Dan Shawler
Subject: International Shipment of Human Specimens
MIME-Version: 1.0
Content-Type: multipart/alternative;
boundary="----_=_NextPart_001_01BE4A2A.599C964E"
This message is in MIME format. Since your mail reader does not understand
this format, some or all of this message may not be legible.
------_=_NextPart_001_01BE4A2A.599C964E
Content-Type: text/plain
We have an investigator that wants to receive a live human tumor biopsy from
a colleague in Spain. She wishes to establish a tumor cell line from the
specimen, so getting the tumor quickly and in good condition is a high
priority. Can someone please let me know how to do this or point me in the
direction of some international shipping regulations?
Thank you for your assistance.
Dan Shawler
------_=_NextPart_001_01BE4A2A.599C964E
Content-Type: text/html
Content-Transfer-Encoding: quoted-printable
International Shipment of Human Specimens
We have an =
investigator that wants to receive a live human tumor biopsy from a =
colleague in Spain. She wishes to establish a tumor cell line =
from the specimen, so getting the tumor quickly and in good condition =
is a high priority. Can someone please let me know how to do this =
or point me in the direction of some international shipping =
regulations?
Thank you for your =
assistance.
Dan =
Shawler
------_=_NextPart_001_01BE4A2A.599C964E--
=========================================================================
Date: Wed, 27 Jan 1999 14:55:01 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Re: International Shipment of Human Specimens
Try these two web sites below:
Bliss M. Schlank
Biosafety Specialist
Zeneca Pharmaceuticals
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185
Fax: 302.886.2909
bliss.schlank@phwilm.
> ----------
> From: Dan Shawler[SMTP:dshawler@]
> Sent: Wednesday, January 27, 1999 2:22 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: International Shipment of Human Specimens
>
> We have an investigator that wants to receive a live human tumor biopsy
> from a colleague in Spain. She wishes to establish a tumor cell line from
> the specimen, so getting the tumor quickly and in good condition is a high
> priority. Can someone please let me know how to do this or point me in
> the direction of some international shipping regulations?
>
> Thank you for your assistance.
>
> Dan Shawler
>
>
>
=========================================================================
Date: Thu, 28 Jan 1999 09:29:48 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Management of MSDS
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
Good day to all:
Question regarding management of MSDS for a small university (1,200 students,
400 employees).
Each department has copies of MSDS for chemicals in their own areas. Copies
are sent to the safety officer and security (the only area where MSDS are
available 24 hours/day, security also covers the phone 24 hours/day).
No campus wide computer network yet that would allow access to a database
(only in certain areas). Slowly being implemented.
Maintain the status quo or any ideas for improvement?
Ed Krisiunas, MT(ASCP), CIC, MPH
Consultant
Spectrum
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
ekrisiunas@
=========================================================================
Date: Thu, 28 Jan 1999 09:35:28 EST
Reply-To: speaker@ehs.psu.edu
Sender: A Biosafety Discussion List
From: Curt Speaker
Organization: University Safety - Penn State Univ
Subject: Re: Management of MSDS
In-Reply-To:
Ed:
There are existing databases of MSDSs that you can tap into that
can provide access to huge amounts of information easily.
There are databases maintained at Cornell Univ., University of
Georgia, University of Vermont, and the Enviro-Net web site to
name just a couple.
Check the Penn State EHS web page at
to see the links that we use.
No sense in reinventing the wheel...
Hope this helps!
Curt
Curt Speaker
Biosafety Officer
Penn State University
Environmental Health and Safety
speaker@ehs.psu.edu
^...^
(O_O)
=(Y)=
"""
=========================================================================
Date: Thu, 28 Jan 1999 08:40:25 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: Management of MSDS
MIME-Version: 1.0
Content-Type: text/plain
Hi, Ed -
Like Curt, I strongly support the idea of on-line MSDS access. At UCSF,
we require that each employee have access to the MSDSs for chemicals in
his or her area. We tell folks they can keep a hardcopy file (which
must be maintained in complete and current form) OR they can provide
workstation access to an on-line source. Since we don't keep an
in-house database, this means a Net-accessible source. Most chemical
companies now have on-line databases for their products. Curt also
mentioned several other such sources maintained by academic
institutions. We do require that lab staff who claim on-line access
know how to use it, so asking for demos of that capability is part of
our audit process. We try to make it easy by asking the labs to
bookmark our EH&S web site () because we have a
page of links to MSDS sources. Janitorial staff (who generally don't
have ready access to a work station) must keep readily available hard
copies in their work areas but the number of MDSDs in their files tends
to be pretty small and product line changes infrequent.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Ed Krisiunas [mailto:EKrisiunas@]
Sent: Thursday, January 28, 1999 6:30 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Management of MSDS
Good day to all:
Question regarding management of MSDS for a small university (1,200
students,
400 employees).
Each department has copies of MSDS for chemicals in their own areas.
Copies
are sent to the safety officer and security (the only area where MSDS
are
available 24 hours/day, security also covers the phone 24 hours/day).
No campus wide computer network yet that would allow access to a
database
(only in certain areas). Slowly being implemented.
Maintain the status quo or any ideas for improvement?
Ed Krisiunas, MT(ASCP), CIC, MPH
Consultant
Spectrum
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
ekrisiunas@
=========================================================================
Date: Thu, 28 Jan 1999 08:47:54 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Alfred L. 'Al' Jin"
Subject: Re: Management of MSDS
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/enriched; charset="us-ascii"
Everyone,
Where to find msds on the internet, try:
for direct access to manufacturers MSDS.
Al Jin, BSO, IH, CBSP, MS, CM(AAM), M(ASCP),
Lawrence Livermore National Laboratory, Livermore, CA, Hazards Control
Department, MS-289,
925 423-7385,
E-mail:Jin2@
>Hi, Ed -
>
>Like Curt, I strongly support the idea of on-line MSDS access. At
UCSF,
>we require that each employee have access to the MSDSs for chemicals
in
>his or her area. We tell folks they can keep a hardcopy file (which
>must be maintained in complete and current form) OR they can provide
>workstation access to an on-line source. Since we don't keep an
>in-house database, this means a Net-accessible source. Most chemical
>companies now have on-line databases for their products. Curt also
>mentioned several other such sources maintained by academic
>institutions. We do require that lab staff who claim on-line access
>know how to use it, so asking for demos of that capability is part of
>our audit process. We try to make it easy by asking the labs to
>bookmark our EH&S web site () because we have
a
>page of links to MSDS sources. Janitorial staff (who generally don't
>have ready access to a work station) must keep readily available hard
>copies in their work areas but the number of MDSDs in their files
tends
>to be pretty small and product line changes infrequent.
>
>-- Glenn
>
>-----------------------------------------------
>Glenn A. Funk, Ph.D., CBSP
>Biosafety Officer
>U.C. San Francisco
>415-476-2097 fax 415-476-0581
>glennf@ehsmail.ucsf.edu
>
>
>-----Original Message-----
>From: Ed Krisiunas [mailto:EKrisiunas@]
>Sent: Thursday, January 28, 1999 6:30 AM
>To: BIOSAFTY@MITVMA.MIT.EDU
>Subject: Management of MSDS
>
>
>Good day to all:
>
>Question regarding management of MSDS for a small university (1,200
>students,
>400 employees).
>
>Each department has copies of MSDS for chemicals in their own areas.
>Copies
>are sent to the safety officer and security (the only area where MSDS
>are
>available 24 hours/day, security also covers the phone 24 hours/day).
>
>No campus wide computer network yet that would allow access to a
>database
>(only in certain areas). Slowly being implemented.
>
>Maintain the status quo or any ideas for improvement?
>
>Ed Krisiunas, MT(ASCP), CIC, MPH
>Consultant
>Spectrum
>115 Lyons Road
>Burlington, Connecticut
>06013
>860-675-1217
>860-675-1311(fax)
>ekrisiunas@
=========================================================================
Date: Thu, 28 Jan 1999 12:40:17 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stefan Wagener
Subject: Re: Management of MSDS
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hi Ed;
If it is not broken and you meet the regulatory requirements, don't fix it.
Your people are obviously used to it, they know where to get them, so don't
mess around with it.
Independent of all computer whiz and whoop you still need hard copies,
especially manufacturer specific copies. No generic MSDS computer CD
database online Web power outage broken monitor spilled coffee can replace
the piece of paper unless you burn it :-)
Did I really write this one????
Stefan
At 09:29 AM 1/28/99 -0500, you wrote:
>Good day to all:
>
>Question regarding management of MSDS for a small university (1,200 students,
>400 employees).
>
>Each department has copies of MSDS for chemicals in their own areas. Copies
>are sent to the safety officer and security (the only area where MSDS are
>available 24 hours/day, security also covers the phone 24 hours/day).
>
>No campus wide computer network yet that would allow access to a database
>(only in certain areas). Slowly being implemented.
>
>Maintain the status quo or any ideas for improvement?
>
>Ed Krisiunas, MT(ASCP), CIC, MPH
>Consultant
>Spectrum
>115 Lyons Road
>Burlington, Connecticut
>06013
>860-675-1217
>860-675-1311(fax)
>ekrisiunas@
=========================================================================
Date: Thu, 28 Jan 1999 15:39:55 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Mike Wendeler
Subject: Bloodborne Pathogens
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: quoted-printable
It's that time of year when I put together my annual bloodborne pathogen =
refresher training. Since this training tends to cover the same topics =
every year, I usually try to throw in some information on newly emerging =
"hot topics" regarding BBP's. Does anyone out there know of any newly =
emerging topics or issues that I could throw in my training this year to =
keep it fresh?
Mike Wendeler
Biosafety Officer
Wyeth-Ayerst Global Pharmaceuticals
=========================================================================
Date: Thu, 28 Jan 1999 16:03:15 -0500
Reply-To: rubockpa@UMDNJ.EDU
Sender: A Biosafety Discussion List
From: Paul Rubock
Organization: eohss-umdnj
Subject: Re: Bloodborne Pathogens
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
How about some HCV information, the quantity of which seems to be
growing geometrically. Try the CDC website for this. I too am always
looking for "new" material for retratining and a fair number of people
in the audience are much less aware of this than HIV or HBV.
Paul Rubock
Lab Safety
UMDNJ
=========================================================================
Date: Thu, 28 Jan 1999 16:06:19 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Gergis, Nasr N."
Subject: Re: Bloodborne Pathogens
MIME-Version: 1.0
Content-Type: text/plain
Mike:
You may try this website, which has Bloodborne Pathogen
nasr
> -----Original Message-----
> From: Mike Wendeler [SMTP:WENDELEM@LABS.]
> Sent: Thursday, January 28, 1999 3:40 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Bloodborne Pathogens
>
> It's that time of year when I put together my annual bloodborne pathogen
> refresher training. Since this training tends to cover the same topics
> every year, I usually try to throw in some information on newly emerging
> "hot topics" regarding BBP's. Does anyone out there know of any newly
> emerging topics or issues that I could throw in my training this year to
> keep it fresh?
>
> Mike Wendeler
> Biosafety Officer
> Wyeth-Ayerst Global Pharmaceuticals
=========================================================================
Date: Thu, 28 Jan 1999 16:07:01 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Gergis, Nasr N."
Subject: Re: Bloodborne Pathogens
MIME-Version: 1.0
Content-Type: text/plain
Mike
you may try this website;
Nasr
> -----Original Message-----
> From: Mike Wendeler [SMTP:WENDELEM@LABS.]
> Sent: Thursday, January 28, 1999 3:40 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Bloodborne Pathogens
>
> It's that time of year when I put together my annual bloodborne pathogen
> refresher training. Since this training tends to cover the same topics
> every year, I usually try to throw in some information on newly emerging
> "hot topics" regarding BBP's. Does anyone out there know of any newly
> emerging topics or issues that I could throw in my training this year to
> keep it fresh?
>
> Mike Wendeler
> Biosafety Officer
> Wyeth-Ayerst Global Pharmaceuticals
=========================================================================
Date: Thu, 28 Jan 1999 13:52:36 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Chris Carlson
Subject: Re: Bloodborne Pathogen "Hot Topics"
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
The State of California is in the process of revising our Bloodborne
Pathogen Standard, and we think it will be become the new model for the
rest of the country. The main changes will be inclusion of Hepatitis C in
all the places where Hepatitis B and HIV are mentioned; required use of
"safer needles" and "needle-less systems"; and keeping a detailed
"needle-stick log".
To see the emergency order adn other resources, go to
and to see the proposed revised standard, go to
There is going to be lots of new stuff to keep our trainees' attention!
Chris
******************************************************************************
Chris Carlson
Biosafety Officer
Office of Environment, Health & Safety
317 University Hall - #1150
University of California
Berkeley, CA 94720-1150
phone: (510) 643-6562
e-mail: ccarlson@uclink4.berkeley.edu
fax: (510) 643-7595
******************************************************************************
Visit our Web Site at
******************************************************************************s
=========================================================================
Date: Thu, 28 Jan 1999 17:38:29 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Esmeralda Party
Subject: Re: Help Please - Disinfectant
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Terry,
I saw that several people responded to your mail with some suggestions.
Where they helpful?
Did you see that Columbia University was cited by OSHA for alleged willful
and other safety and health violations with $77,500 in penalties? You can
find more details in
This might have prompted the administration to act with respect to Ed
Christman, because although the citation appeared only Monday the
inspection by OSHA on July 23, 1998.
Esmeralda
At 01:35 PM 1/26/1999 PST, you wrote:
>Hi All,
>Problem I need the name of a commerical cleaning product that the
>manufacturer agress will kill hepatitis and staph and is presuemable is
>registered as a disinfectant. Please respond directly to me at
>terystim@
>
>Background- A sanitary line backed up into a grease interceptor and
>floor drains in a cafeteria on site. Found and severed the plumbing
>connection which did not appear on any building plans. Contractor
>immediately acted and had all floors traps etc. cleaned by a reputable
>company using a product called Milgro by DriEaz. Then the contractor
>contacted the municpal health inspector to reopen the cafeteria.
>
>Health inspector stated correctly that Dri Eaz does not ceritify this
>product to kill hepatitis but will certify for staph which the inspector
>is concerned about. It is more than a WEEK since cleaning and time alone
>(lost by the usual finger pointing and poor communication)is resolving
>any residual helath issues.
>
>The inspector rightly refuses to reopen. However he will NOT accept
>1:10 bleach and will not provide the name of any acceptable product that
>is "certified" (his words) for hepatitis. I presume he means a product
>that is registered as a viricial disinfectant that the mfgr will state
>kills hep A.
>
>I need the name of a product that is "certifed" for hepatitis (inspector
>is not distinguishing among the viruses) to staisfy the municpal health
>inspector. We need to get this cafe reopened. Of course I'm away from
>my office. Thanks again for any help you can give.
>Terry Stimpfel - Montclair State Univ
>
>Terry
>
>______________________________________________________
>Get Your Private, Free Email at
>
>
=========================================================================
Date: Thu, 28 Jan 1999 15:49:34 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Alfred L. 'Al' Jin"
Subject: Re: Bloodborne Pathogens
In-Reply-To:
Mime-Version: 1.0
Content-Type: multipart/mixed;
boundary="============_-1294558720==_============"
--============_-1294558720==_============
Content-Type: text/plain; charset="us-ascii"
Everyone,
I found the September 16th, 1998 issue of MMWR (Vol 47, RR-19)
entitled,"Recommendations for Prevention and Control of Hepatitis C (HCV)
Infections and Related Chronic Diseases" very useful. For those who missed
it, see the enclosed pdf file. Please direct all questions to CDC.
Al Jin, BSO, IH, CBSP, MS, CM(AAM), M(ASCP),
Lawrence Livermore National Laboratory, Livermore, CA,
Hazards Control Department, MS-289,
925 423-7385, E-mail:Jin2@
>It's that time of year when I put together my annual bloodborne pathogen
>refresher training. Since this training tends to cover the same topics
>every year, I usually try to throw in some information on newly emerging
>"hot topics" regarding BBP's. Does anyone out there know of any newly
>emerging topics or issues that I could throw in my training this year to
>keep it fresh?
>
>Mike Wendeler
>Biosafety Officer
>Wyeth-Ayerst Global Pharmaceuticals
--============_-1294558720==_============
Content-Type: application/mac-binhex40; name="HCV.PDF"
Content-Disposition: attachment; filename="HCV.PDF"
Attachment Converted: "E:\Eudora\Attach\HCV.PDF"
--============_-1294558720==_============--
=========================================================================
Date: Thu, 28 Jan 1999 18:42:10 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Re: Bloodborne Pathogen "Hot Topics"
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
If one looks back to the Preamble of the OSHA Bloodborne Pathogen Standard
from December 0f 1991, in addition to HIV and HBV, Non-A, Non-B Hepatitis,
CJD, Brucellosis, malaria, and other disease are included as bloodborne
pathogens.
How many of us were actually discussing these diseases in 1992 as part of our
training?
Hepatitis C - virus dujour for the late 1990's!
Ed Krisiunas, MT(ASCP), CIC, MPH
Spectrum
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
ekrisiunas@
=========================================================================
Date: Fri, 29 Jan 1999 08:43:51 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Debra Hunt
Subject: Re: Bloodborne Pathogens
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Suggestions about hepatitis C are good ones. I have found that providing
bloodborne pathogen exposure data for my institution each year interests
the audience (particularly the physicians who know everything about
bloodborne pathogens anyway, right?). I try to provide
departmental-specific exposure information...how many needlesticks occur,
how they occur, other cuts, etc., how many exposures occurred to HIV, HCV,
or HBV positive patients, etc....and, unfortunately, we discuss how our
cases of occupationally-acquired hep c infections are occurring. This
tends to put them on alert and probably does more to help them prevent
infections than any discussions about general epidemiology. It's also a
good way to prepare the audience to be more receptive to the increased
costs associated with safety devices.
Debbie Hunt, DrPH, RBP, CBSP
Director, Biological Safety
Duke University/Duke University Health Systems
Durham, NC
919-684-8822
fax:919-681-7509
hunt0009@mc.duke.edu
=========================================================================
Date: Fri, 29 Jan 1999 09:28:37 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Gergis, Nasr N."
Subject: Re: Bloodborne Pathogens
MIME-Version: 1.0
Content-Type: text/plain
> To everyone:
>
For Hepatitis C and other strains: You may review the website:
>
>
> Nasr
>
>
> Suggestions about hepatitis C are good ones. I have found that providing
> bloodborne pathogen exposure data for my institution each year interests
> the audience (particularly the physicians who know everything about
> bloodborne pathogens anyway, right?). I try to provide
> departmental-specific exposure information...how many needlesticks occur,
> how they occur, other cuts, etc., how many exposures occurred to HIV, HCV,
> or HBV positive patients, etc....and, unfortunately, we discuss how our
> cases of occupationally-acquired hep c infections are occurring. This
> tends to put them on alert and probably does more to help them prevent
> infections than any discussions about general epidemiology. It's also a
> good way to prepare the audience to be more receptive to the increased
> costs associated with safety devices.
>
> Debbie Hunt, DrPH, RBP, CBSP
> Director, Biological Safety
> Duke University/Duke University Health Systems
> Durham, NC
> 919-684-8822
> fax:919-681-7509
> hunt0009@mc.duke.edu
=========================================================================
Date: Fri, 29 Jan 1999 07:48:09 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Sandra Fry
Subject: Re: Management of MSDS
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; CHARSET=ISO-8859-1
Nicely said, Stefan :-)
I agree, a hard copy must be maintained on site in the event computer system
is down. Up where I live, this is a requirement.
What we do...use an off site tracking company. For $2 per MSDS / year they
send us updates 4X a year , they come to my office, I distribute them to the
appropriate labs and they update the binders (with the manufacturers MSDS,
not generic). It works.
Sandy
> Hi Ed;
>
> If it is not broken and you meet the regulatory requirements, don't fix
it.
> Your people are obviously used to it, they know where to get them, so
don't
> mess around with it.
>
> Independent of all computer whiz and whoop you still need hard copies,
> especially manufacturer specific copies. No generic MSDS computer CD
> database online Web power outage broken monitor spilled coffee can replace
> the piece of paper unless you burn it :-)
>
> Did I really write this one????
>
> Stefan
>
>
>
> At 09:29 AM 1/28/99 -0500, you wrote:
> >Good day to all:
> >
> >Question regarding management of MSDS for a small university (1,200
students,
> >400 employees).
> >
> >Each department has copies of MSDS for chemicals in their own areas.
Copies
> >are sent to the safety officer and security (the only area where MSDS are
> >available 24 hours/day, security also covers the phone 24 hours/day).
> >
> >No campus wide computer network yet that would allow access to a database
> >(only in certain areas). Slowly being implemented.
> >
> >Maintain the status quo or any ideas for improvement?
> >
> >Ed Krisiunas, MT(ASCP), CIC, MPH
> >Consultant
> >Spectrum
> >115 Lyons Road
> >Burlington, Connecticut
> >06013
> >860-675-1217
> >860-675-1311(fax)
> >ekrisiunas@
---------------End of Original Message-----------------
Sandra C. Fry
Biosafety Officer
BC Centre for Disease Control,
655 W. 12th Avenue
Vancouver, BC V5Z 4R4
phone: (604) 660 6167
pager: (604) 893 5737
fax: (604) 660 6073
=========================================================================
Date: Fri, 29 Jan 1999 11:16:04 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: Bloodborne Pathogens
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Mike,
I always include case studies in my training. It brings the "point" home
and allows them to practice what to do when there is an exposure incident.
Case studies can be found at both CDC and OSHA sites.
Hep C is certainly an emerging risk, trainees were also interested that
malaria and syphillus are sometimes transmitted occupationally in the US.
At 03:39 PM 1/28/99 -0500, you wrote:
>It's that time of year when I put together my annual bloodborne pathogen
refresher training. Since this training tends to cover the same topics
every year, I usually try to throw in some information on newly emerging
"hot topics" regarding BBP's. Does anyone out there know of any newly
emerging topics or issues that I could throw in my training this year to
keep it fresh?
>
>Mike Wendeler
>Biosafety Officer
>Wyeth-Ayerst Global Pharmaceuticals
>
>
Janice Flesher, MS
Principal Industrial Hygienist
Environmental & Occupational Health & Safety Service
University Medical Dental School of New Jersey
97 Paterson Street #227
New Brunswick, NJ 08901
732-235-8497 phone
732-235-8499 fax
=========================================================================
Date: Fri, 29 Jan 1999 14:29:14 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: Bloodborne Pathogen "Hot Topics"
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Ed and Mike:
Ed makes an excellent point. There are a host of other BBPs that OSHA
says trainers must present to workers at risk that almost never get any
attention or verbal citation to trainees. Each is interesting and some
are significant biohazards. Signs and symptoms are commonly ignored even
on the biggees like HIV and HBV and HCV. OSHA requires careful attention
to this problem area. Give them a handout of BBP organisms and signs and
symptoms and go over them citing historical lab exposure reports.
I encourage all to go over the basics
periodically and, while fresh news is more catchy, a walk through your
mail room or serological labs, medical waste areas, autoclave areas, and
blood labs can be down right humilating if you don't do frequent
biosafety audits and relate the results back to the staff. Even if you do,
you often find silly, but dangerous problems. One such walk around can
give you plenty to talk about.
Also, how good is "your" institute's or company's biosafety /BBP
registration process and approval system for work with BBP? If you have
clinical labs, how do they stack up? Sometimes you can find problems
from the personnel simply by going over the outline of your ECP and basic
UPs and fielding their questions. If you are enthusiastic about doing BBP
training, the workers will respond more enthusiastically. I sometimes
use a medical school teaching gimmick, by having a trainee come forward
and read some Pretend Problems or symptoms they are having and then
dissect the problem in an orderly way asking the audience to
participate. It is actually fun!
I also find that some supervisors need more training and re-training in
BBP and good microbiological techniques than the lab workers do! Many
supervisors don't know their importance in the scheme of biosafety
training which always must be supervisor driven and supervisor monitored.
Joe Coggin
On Thu, 28 Jan 1999, Ed Krisiunas wrote:
> If one looks back to the Preamble of the OSHA Bloodborne Pathogen Standard
> from December 0f 1991, in addition to HIV and HBV, Non-A, Non-B Hepatitis,
> CJD, Brucellosis, malaria, and other disease are included as bloodborne
> pathogens.
>
> How many of us were actually discussing these diseases in 1992 as part of our
> training?
>
>
> Hepatitis C - virus dujour for the late 1990's!
>
> Ed Krisiunas, MT(ASCP), CIC, MPH
> Spectrum
> Burlington, Connecticut
> 06013
> 860-675-1217
> 860-675-1311(fax)
> ekrisiunas@
>
=========================================================================
Date: Fri, 29 Jan 1999 17:13:51 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Christopher Brey
Subject: Tissue Homogenization & Fix
Dear Biosaftyers:
I am looking for a tissue homogenizer for processing human tissue samples,
volume with fixative not to exceed 10-20 ml. Can somebody make a specific
recommendation for a homogenizer that could: 1) handle such a volume; 2) keep
aerosol formation to a minimum and; 3) not occupy a lot of space within a
biosafety cabinet.
Additionally, I would appreciate information on the disinfectant properties of
guanidine hcl when used as a fixitive during homogenization. What amount of
contact time is required to inactivate pathogens with guanidine? Is guanidine
alone adequate or should another chemical agent be added during homogenization
to ensure pathogen inactivation?
Thanks for your help!
Christopher Brey
EH&S Department
DNAX Research Institute
brey@
=========================================================================
Date: Fri, 29 Jan 1999 17:36:56 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Chris Carlson
Subject: Re: Bloodborne Pathogen "Hot Topics"
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
The State of California is in the process of revising our Bloodborne
Pathogen Standard, and we think it will be become the new model for the
rest of the country. The main changes will be inclusion of Hepatitis C in
all the places where Hepatitis B and HIV are mentioned; required use of
"safer needles" and "needle-less systems"; and keeping a detailed
"needle-stick log".
To see the emergency order and other resources, go to
and to see the proposed revised standard, go to
There is going to be lots of new stuff to keep our trainees' attention!
Chris
******************************************************************************
Chris Carlson
ccarlson@uclink4.berkeley.edu
******************************************************************************
=========================================================================
Date: Sun, 31 Jan 1999 21:15:56 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: Introduction
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
Hello Biosafety,
This is my first visit to the list. I'd like to introduce myself.
I'm the Director (half-time) of the Laboratory Safety Workshop (Natick, MA).
LSW is a non-profit educational organization specializing in safety in science
education. I also do some work privately as a lab safety consultant.
LSW publishes a newsletter , Speaking of Safety, three time a year, operates
an AV-lending library, and provides seminar and short courses throughout the
world.
This Tuesday, I'll be talking to some Massachusetts Biotech Council member on
"How to Convince People that You are Serious About Safety (And, that It's
important).
LSW has it's own discussion list, LABSAFETY-L for member of the National
Association of Chemical Hygiene Officers (NACHO) and anyone interested in lab
safety issues.
I'm looking forward to learning more about biosafety issues here and sticking
in $0.02 once in a while. ... jim
*****************************************************
James A. Kaufman, President
The Laboratory Safety Workshop
Safety in Science Education
192 Worcester Road, Natick, MA 01760
508-647-1900 Fax: 508-647-0062 labsafe@
LSW is a national non-profit educational organization dedicated to making
health and safety an integral and important part of science education. Free
copies of our Laboratory Safety Guidelines, seminar schedule, Publications
List, AV-Lending Library List, and membership information are available on
request. The LABSAFETY-L discussion list is a public service of LSW.
******************************************************
=========================================================================
Date: Mon, 1 Feb 1999 09:38:07 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph P. Kozlovac"
Subject: Origin of an Old Biosafety Quotation
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hello all,
I am curious as to the origin of a quotation which recently appeared in
Performance Review, Vol 6, Number 3 (The Journal of the Controlled
Environment Testing Association).
The article entitled "Biological Safety Principles Enhance Contamination
Control" by Robert W. Powitz contained the quote,
"It is true that good engineering design is not a completely adequate
substitute for thoughtfully applied good technique, but the reverse also is
true. Both are necessary."
Dr. Powitz in his article stated prior to the quote..."Thus, the art and
science of biological safety wrote...
I would like to know if any of you know the origin of that particular
quotation.
Thanks in advance
______________________________________________________________________________
Biological Safety Officer
Safety and Environmental Protection Program
NCI - Frederick Cancer Research
and Development Center
(301)846-1451 fax: (301)846-6619
email: jkozlovac@mail.
______________________________________________________________________________
=========================================================================
Date: Mon, 1 Feb 1999 09:05:19 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: ventilation issues
MIME-Version: 1.0
Content-Type: text/plain
Can someone recommend a reference that would cover the operation of fan coil
units as part of the ventilation system?
also:
If you have a BSL2 lab, where would you put the HEPA filters in the supply
air system, if you in fact wanted them? how often would you change them
out?
and:
for human somatic cell therapy processes, where you have a BSC, need to
store supplies segregated from other lab supplies, and run a couple of CO2
incubators...
is there a requirement for a clean room operation for this type of process?
=========================================================================
Date: Mon, 1 Feb 1999 16:52:06 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Noel Neighbor
Subject: Re: ventilation issues
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Industrial Ventilation by the American Conference of Governmental
Industrial Hygienists might be of help.
My opinion is that it is better to put the HEPA unit downstream of the fan
coil unit. That way, if the fan coil unit enclosure leaks on the suction
side of the fan, you will be able to clean this air along with the main
stream of air. If the HEPA unit is upstream of the fan coil unit and the
enclosure is leaky, you will contaminate the already filtered air and
make the 99.97% efficiency an inaccurate number relative to what is
flowing into the lab. If you do it this way, though, you will have to do
a good job of filtering the air before it enters the fan coil so as to
avoid dust deposits on the coils. Less than 99.97% is fine for this,
though.
On Mon, 1 Feb 1999, Therese M. Stinnett wrote:
> Can someone recommend a reference that would cover the operation of fan coil
> units as part of the ventilation system?
>
> also:
> If you have a BSL2 lab, where would you put the HEPA filters in the supply
> air system, if you in fact wanted them? how often would you change them
> out?
>
> and:
> for human somatic cell therapy processes, where you have a BSC, need to
> store supplies segregated from other lab supplies, and run a couple of CO2
> incubators...
> is there a requirement for a clean room operation for this type of process?
>
Noel Neighbor, Research Associate
Poultry Health Laboratory
Center of Excellence for Poultry Science
University of Arkansas 72701
(501) 575-8493
(501) 575-8490 Fax
=========================================================================
Date: Mon, 1 Feb 1999 22:05:40 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "J.H. Keene"
Subject: Re: ventilation issues
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Therese, Re the HEPA's for supply air, check with Jim Grantham at Certek,
Inc. in North Carolina. He has some units that would fit this use. Also,
places that handle human transplant tissues such as bone, etc. use the
terminal HEPA's on the exhaust and we used them when I was at Abbott in some
of our fill rooms. As to when they should be changed, that would be when
they begin to load up with dirt and that would depend on the condition of
the air going to them. In most areas where they are used, there is
recirculation of air and eventually it get's pretty clean so they last a
long time. If it's once through air as it would be in a lab, then you will
have to play it by ear and see how long it takes. The one thing you don't
have to do with them is decon them since it is normal air coming in. Best I
can do. good luck. Jack
John H. Keene, Dr. P.H.
Biohaztec Associates, Inc.
-----Original Message-----
From: Therese M. Stinnett
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Monday, February 01, 1999 6:29 PM
Subject: ventilation issues
>Can someone recommend a reference that would cover the operation of fan
coil
>units as part of the ventilation system?
>
>also:
>If you have a BSL2 lab, where would you put the HEPA filters in the supply
>air system, if you in fact wanted them? how often would you change them
>out?
>
>and:
>for human somatic cell therapy processes, where you have a BSC, need to
>store supplies segregated from other lab supplies, and run a couple of CO2
>incubators...
>is there a requirement for a clean room operation for this type of process?
=========================================================================
Date: Tue, 2 Feb 1999 11:46:25 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Mildew Problem
MIME-Version: 1.0
Content-Type: text/plain
I'm sure some of my fellow BIOSAFTYers have encountered this problem. I
haven't (until now) so I'd appreciate your help.
During the power outage that hit the San Francisco Bay Area in December,
one of our med center offices developed a steam register leak that
soaked the carpet. As you can imagine, it didn't take long before the
secretary in that office began to complain about mildew odors, then
upper respiratory reactions. She was moved temporarily to another space
and the carpeting and padding removed from her office. The floor has
been mopped with bleach solution but our IH asks if that is enough. My
strong suspicion is that it isn't an adequate decontamination and that
there should probably be a fungicide with long-duration residual action
applied to the floor before new carpeting is laid.
Can you recommend any specific products in this case? Are there other
decon or preparation activities that should be done here before
returning the office to its former use?
Thanks in advance for your help.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
=========================================================================
Date: Tue, 2 Feb 1999 14:50:31 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Francis Churchill
Subject: Re: Mildew Problem
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
We have had similar situations and spent all of our time trying to clean
the carpet to no avail. Now we just replace the carpet and that seems to be
sufficient at alleviating the symptoms. We try to avoid putting more
chemicals, such as disinfectants, into the mix. It's been effective so
far, though I have no hard data about microbial growth to back it up.
Alcohol and calculus don't mix. Never drink and derive.
Francis Churchill, IHIT
University of Vermont - Environmental Safety Facility
655D Spear Street, Burlington, VT 05405-3010
(802) 656-5405
fchurchi@zoo.uvm.edu
=========================================================================
Date: Tue, 2 Feb 1999 14:54:06 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: James Scott
Organization: University of Toronto Botany
Subject: Re: Mildew Problem
Wet carpets are generally bad things from an IAQ perspective, both
due to associated malodors as well as other, potentially noxious
microbial compounds such as mycotoxins and VOCs. Several fungal
genera noted for the production of profoundly toxic mycotoxins are
common to this habitat (e.g. Aspergillus, Fusarium, Penicillium).
While disinfection (i.e. treatment with a biocide) may stave the
growth of these organisms, it does little or nothing to address the
protential presence of mycotoxic compounds already excreted into the
material (not to mention contributing yet another chemical to the
soup). The best advice is to remove the carpet if it cannot be dried
within 2-3 days. Many commonly used biocides are next to
useless against microfungi in any case. And the application
of these compounds (usually in aqueous solution) do little more than
contribute more water to the problem.
The removal of a contaminated carpet requires some care if it is
likely to be contaminated with a mycotoxigenic fungus. If a
sufficient area is affected, procedures adapted from level 3 asbestos
removal protocols are adopted.
James
--
James Scott -- Department of Botany, University of Toronto
25 Willcocks Street, Toronto ON M5S 3B2 CANADA
Tel: +1 416 978-5563 /-3543 Fax: +1 416 978-5878
Fungal ecology/taxonomy, medical mycology, biodeterioration, IAQ, OH&S
Date sent: Tue, 2 Feb 1999 11:46:25 -0800
Send reply to: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Mildew Problem
To: BIOSAFTY@MITVMA.MIT.EDU
I'm sure some of my fellow BIOSAFTYers have encountered this problem. I
haven't (until now) so I'd appreciate your help.
During the power outage that hit the San Francisco Bay Area in December,
one of our med center offices developed a steam register leak that
soaked the carpet. As you can imagine, it didn't take long before the
secretary in that office began to complain about mildew odors, then
upper respiratory reactions. She was moved temporarily to another space
and the carpeting and padding removed from her office. The floor has
been mopped with bleach solution but our IH asks if that is enough. My
strong suspicion is that it isn't an adequate decontamination and that
there should probably be a fungicide with long-duration residual action
applied to the floor before new carpeting is laid.
Can you recommend any specific products in this case? Are there other
decon or preparation activities that should be done here before
returning the office to its former use?
Thanks in advance for your help.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
=========================================================================
Date: Tue, 2 Feb 1999 15:24:19 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "J.H. Keene"
Subject: Re: Mildew Problem
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
If the carpet and padding has been removed and the floor treated with
bleach, then dried, everything should be OK. Is the floor a concrete slab
or wood flooring? Sometimes a relatively porous material like wood could
take up the water and/or disinfectant and stay wet for an extended period.
The key is to make sure that the leak is fixed and the flooring is dry, then
new carpet can be layed and all should be well.
I also note on one of the other replies that if the agent is "toxigenic" the
contaminated carpet should be removed using Asbestos abatement methods. I
know that some have recommended this, but personally think that such a
requirement is a bit of overkill. I'd like to see some discussion of such
recommendations based on sound biological safety principles and science, not
on the basis of a recommendation made, essentially, by a single individual
and picked up by others. Any comments?
-----Original Message-----
From: Funk, Glenn
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Tuesday, February 02, 1999 2:44 PM
Subject: Mildew Problem
>I'm sure some of my fellow BIOSAFTYers have encountered this problem. I
>haven't (until now) so I'd appreciate your help.
>
>During the power outage that hit the San Francisco Bay Area in December,
>one of our med center offices developed a steam register leak that
>soaked the carpet. As you can imagine, it didn't take long before the
>secretary in that office began to complain about mildew odors, then
>upper respiratory reactions. She was moved temporarily to another space
>and the carpeting and padding removed from her office. The floor has
>been mopped with bleach solution but our IH asks if that is enough. My
>strong suspicion is that it isn't an adequate decontamination and that
>there should probably be a fungicide with long-duration residual action
>applied to the floor before new carpeting is laid.
>
>Can you recommend any specific products in this case? Are there other
>decon or preparation activities that should be done here before
>returning the office to its former use?
>
>Thanks in advance for your help.
>
>-- Glenn
>
>-----------------------------------------------
>Glenn A. Funk, Ph.D., CBSP
>Biosafety Officer
>U.C. San Francisco
>415-476-2097 fax 415-476-0581
>glennf@ehsmail.ucsf.edu
>
=========================================================================
Date: Tue, 2 Feb 1999 16:10:32 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Larry Thompson
Subject: Re: Mildew Problem
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
>Can you recommend any specific products in this case? Are there other
>decon or preparation activities that should be done here before
>returning the office to its former use?
Dr. Funk, et al
Our experience has been that if the carpet cannot be thoroughly dried
within several hours of having been water soaked, the best decision is to
replace it. In our facilities if the wet carpet has had 12-24 hours to
"incubate", we have never been successful in preventing the mold/mildew
problems. Even after an initial "success", some nasty problems have been
delayed until the return of warm weather and increases in humidity. A
springtime surprise!
TTFN,
Larry
Larry J. Thompson, DVM PhD DABVT
Certified Biological Safety Specialist
Director of Biosafety
College of Veterinary Medicine-Cornell University
Upper Tower Road phone 607-253-3900
Ithaca, NY 14853 fax 607-253-3943
"How old would you be if you didn't know when you was born?"
Satchel Paige
=========================================================================
Date: Tue, 2 Feb 1999 15:12:16 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: Mildew Problem
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Well, I consider myself an expert on mildew - since I probably cleaned more
moldy shower stalls than all you males combined!
First of all the asbestos abatement thingy? (I agree with Jack, that's
overkill) - unless it's right next to a patient waiting room full of
immunosuppressed. I'd just have the cleaning staff wear a particulate
disposable respirator. Don't think VOCs would be that high. If it's been
fermenting a while, then maybe a full-face canister with a HEPA/Charcoal
combo is needed.
Secondly, my personal experience with moldy shower stalls and other places
- like tissue culture labs - has led me to believe that just killing spores
with bleach or other disinfectant, is not enough. You've got to get rid of
the residual organic matter (dead mold cells) --> eliminate the carbon
source, to really deter mold from coming back. For shower stalls, this
means the old toothbrush and TSP (trisodium phosphate). You can buy big
jugs of TSP at the hardware store, real cheap. It oxidizes the carbon.
Then wipe it up. No (or little) carbon source - Voila - no mold regrowth!
Of course, absolute elimination of all the moisture (check behind the wall
boards) has to be done first. You might as well seal the floor &/or wall
with a moisture repellant sealer while you're at it, b/f putting the new
carpet and pad back down.
Take it from a Houstonian - mold is with us to stay! With the above
technique, I finally have my shower stall routine down to a once/year
scrubbing.
See you can learn valuable scientific information while cleaning house.
Judy Pointer
=========================================================================
Date: Wed, 3 Feb 1999 07:04:26 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: LSW Seeks Host Sites
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
The Laboratory Safety Workshop is looking for academic
institutions in Seattle and Minneapolis areas to host its
summer program. The 24-hour, four-day lab safety short
course is for secondary and post-secondary science
educators.
The proposed dates are June 16-19 and August 16-19
for Seattle and Minneapolis, respectively.
Please contact Tricia McGann, Membership Services
Manager for more information. LSWPFM@ or
1-800-647-1977,
Thanks, ... Jim
*****************************************************
James A. Kaufman, President
The Laboratory Safety Workshop
Safety in Science Education
192 Worcester Road, Natick, MA 01760
508-647-1900 Fax: 508-647-0062 labsafe@
LSW is a national non-profit educational organization dedicated to making
health and safety an integral and important part of science education. Free
copies of our Laboratory Safety Guidelines, seminar schedule, Publications
List, AV-Lending Library List, and membership information are available on
request. The LABSAFETY-L discussion list is a public service of LSW.
******************************************************
=========================================================================
Date: Wed, 3 Feb 1999 09:02:09 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Mildew Problem
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hi Glenn,
I thought I would give you one more response. Chlorine bleach is great so
long as the floor is non-porous. If porous and the mold has made it from
the carpeting into the flooring, no chemical treatment will reach all of
the fungi. Best in that case would be to seal the floor and try to keep
the RH down forever. I am leary of using products that leave a residue as
walking on the surface will cause some of the residue to get airborne. We
had a case here where the office had a cleaning co. come in and use a quat.
Turned out that they were more sensitive to the quat then the original
fungus. The two most common disinfectants that leave a residue are:
quaternary ammoniums and phenolic based ones. I would HEPA vac. the area
after disinfection to remove the dead fungi and reduce possible allergic
reaction.
The chance that you will have significant toxic compounds after a short
incubation is unlikely.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Wed, 3 Feb 1999 13:27:12 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Help Please - Disinfectant
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Terry,
Enclosed is a copy of the EPA's list of approved Tuberculocidal
Disinfectants. This list as well as the EPA's list of approved
Tuberculocidal Sterilants is approved for disinfection of BBP. I do not
understand why you were cited for using bleach. It is also approved(10%).
In fact it is on this list
Your problem could have been caused by a state regulator. Ohio only allows
the disinfectant list or a 15% bleach solution.
Check your state regs.
BTW, I obtained this list off of the web. I could not locate the addres for
you. I believe it is a University in Oregon under contract to the EPA.
Don't ask the EPA. They don't seem to know this list exists:)
Bob
>Terry,
>
>I saw that several people responded to your mail with some suggestions.
>Where they helpful?
>
>Did you see that Columbia University was cited by OSHA for alleged willful
>and other safety and health violations with $77,500 in penalties? You can
>find more details in
>This might have prompted the administration to act with respect to Ed
>Christman, because although the citation appeared only Monday the
>inspection by OSHA on July 23, 1998.
>
>Esmeralda
>
>
>
>
>At 01:35 PM 1/26/1999 PST, you wrote:
>>Hi All,
>>Problem I need the name of a commerical cleaning product that the
>>manufacturer agress will kill hepatitis and staph and is presuemable is
>>registered as a disinfectant. Please respond directly to me at
>>terystim@
>>
>>Background- A sanitary line backed up into a grease interceptor and
>>floor drains in a cafeteria on site. Found and severed the plumbing
>>connection which did not appear on any building plans. Contractor
>>immediately acted and had all floors traps etc. cleaned by a reputable
>>company using a product called Milgro by DriEaz. Then the contractor
>>contacted the municpal health inspector to reopen the cafeteria.
>>
>>Health inspector stated correctly that Dri Eaz does not ceritify this
>>product to kill hepatitis but will certify for staph which the inspector
>>is concerned about. It is more than a WEEK since cleaning and time alone
>>(lost by the usual finger pointing and poor communication)is resolving
>>any residual helath issues.
>>
>>The inspector rightly refuses to reopen. However he will NOT accept
>>1:10 bleach and will not provide the name of any acceptable product that
>>is "certified" (his words) for hepatitis. I presume he means a product
>>that is registered as a viricial disinfectant that the mfgr will state
>>kills hep A.
>>
>>I need the name of a product that is "certifed" for hepatitis (inspector
>>is not distinguishing among the viruses) to staisfy the municpal health
>>inspector. We need to get this cafe reopened. Of course I'm away from
>>my office. Thanks again for any help you can give.
>>Terry Stimpfel - Montclair State Univ
>>
>>Terry
>>
>>______________________________________________________
>>Get Your Private, Free Email at
>>
>>
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Wed, 3 Feb 1999 13:16:15 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: Re: Help Please - Disinfectant
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
FYI, the web address for the EPA's lists of approved disinfectants is
-----Original Message-----
From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
Sent: Wednesday, February 03, 1999 12:27 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: Help Please - Disinfectant
Terry,
Enclosed is a copy of the EPA's list of approved Tuberculocidal
Disinfectants. This list as well as the EPA's list of approved
Tuberculocidal Sterilants is approved for disinfection of BBP. I do not
understand why you were cited for using bleach. It is also approved(10%).
In fact it is on this list
Your problem could have been caused by a state regulator. Ohio only allows
the disinfectant list or a 15% bleach solution.
Check your state regs.
BTW, I obtained this list off of the web. I could not locate the addres for
you. I believe it is a University in Oregon under contract to the EPA.
Don't ask the EPA. They don't seem to know this list exists:)
Bob
>Terry,
>
>I saw that several people responded to your mail with some suggestions.
>Where they helpful?
>
>Did you see that Columbia University was cited by OSHA for alleged willful
>and other safety and health violations with $77,500 in penalties? You can
>find more details in
>This might have prompted the administration to act with respect to Ed
>Christman, because although the citation appeared only Monday the
>inspection by OSHA on July 23, 1998.
>
>Esmeralda
>
>
>
>
>At 01:35 PM 1/26/1999 PST, you wrote:
>>Hi All,
>>Problem I need the name of a commerical cleaning product that the
>>manufacturer agress will kill hepatitis and staph and is presuemable is
>>registered as a disinfectant. Please respond directly to me at
>>terystim@
>>
>>Background- A sanitary line backed up into a grease interceptor and
>>floor drains in a cafeteria on site. Found and severed the plumbing
>>connection which did not appear on any building plans. Contractor
>>immediately acted and had all floors traps etc. cleaned by a reputable
>>company using a product called Milgro by DriEaz. Then the contractor
>>contacted the municpal health inspector to reopen the cafeteria.
>>
>>Health inspector stated correctly that Dri Eaz does not ceritify this
>>product to kill hepatitis but will certify for staph which the inspector
>>is concerned about. It is more than a WEEK since cleaning and time alone
>>(lost by the usual finger pointing and poor communication)is resolving
>>any residual helath issues.
>>
>>The inspector rightly refuses to reopen. However he will NOT accept
>>1:10 bleach and will not provide the name of any acceptable product that
>>is "certified" (his words) for hepatitis. I presume he means a product
>>that is registered as a viricial disinfectant that the mfgr will state
>>kills hep A.
>>
>>I need the name of a product that is "certifed" for hepatitis (inspector
>>is not distinguishing among the viruses) to staisfy the municpal health
>>inspector. We need to get this cafe reopened. Of course I'm away from
>>my office. Thanks again for any help you can give.
>>Terry Stimpfel - Montclair State Univ
>>
>>Terry
>>
>>______________________________________________________
>>Get Your Private, Free Email at
>>
>>
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental
Safety
=========================================================================
Date: Wed, 3 Feb 1999 16:44:01 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Help Please - Disinfectant
In-Reply-To:
Mime-Version: 1.0
Content-Type: multipart/mixed;
boundary="============_-1294044243==_============"
--============_-1294044243==_============
Content-Type: text/plain; charset="us-ascii"
Sorry, I forgot to include the attachment. oops!
I have on this post.
Bob
>FYI, the web address for the EPA's lists of approved disinfectants is
>
>
>-----Original Message-----
>From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
>Sent: Wednesday, February 03, 1999 12:27 PM
>To: BIOSAFTY@MITVMA.MIT.EDU
>Subject: Re: Help Please - Disinfectant
>
>
>Terry,
>
>Enclosed is a copy of the EPA's list of approved Tuberculocidal
>Disinfectants. This list as well as the EPA's list of approved
>Tuberculocidal Sterilants is approved for disinfection of BBP. I do not
>understand why you were cited for using bleach. It is also approved(10%).
>In fact it is on this list
>
>Your problem could have been caused by a state regulator. Ohio only allows
>the disinfectant list or a 15% bleach solution.
>
>Check your state regs.
>
>BTW, I obtained this list off of the web. I could not locate the addres for
>you. I believe it is a University in Oregon under contract to the EPA.
>Don't ask the EPA. They don't seem to know this list exists:)
>
>Bob
>
>>Terry,
>>
>>I saw that several people responded to your mail with some suggestions.
>>Where they helpful?
>>
>>Did you see that Columbia University was cited by OSHA for alleged willful
>>and other safety and health violations with $77,500 in penalties? You can
>>find more details in
>>This might have prompted the administration to act with respect to Ed
>>Christman, because although the citation appeared only Monday the
>>inspection by OSHA on July 23, 1998.
>>
>>Esmeralda
>>
>>
>>
>>
>>At 01:35 PM 1/26/1999 PST, you wrote:
>>>Hi All,
>>>Problem I need the name of a commerical cleaning product that the
>>>manufacturer agress will kill hepatitis and staph and is presuemable is
>>>registered as a disinfectant. Please respond directly to me at
>>>terystim@
>>>
>>>Background- A sanitary line backed up into a grease interceptor and
>>>floor drains in a cafeteria on site. Found and severed the plumbing
>>>connection which did not appear on any building plans. Contractor
>>>immediately acted and had all floors traps etc. cleaned by a reputable
>>>company using a product called Milgro by DriEaz. Then the contractor
>>>contacted the municpal health inspector to reopen the cafeteria.
>>>
>>>Health inspector stated correctly that Dri Eaz does not ceritify this
>>>product to kill hepatitis but will certify for staph which the inspector
>>>is concerned about. It is more than a WEEK since cleaning and time alone
>>>(lost by the usual finger pointing and poor communication)is resolving
>>>any residual helath issues.
>>>
>>>The inspector rightly refuses to reopen. However he will NOT accept
>>>1:10 bleach and will not provide the name of any acceptable product that
>>>is "certified" (his words) for hepatitis. I presume he means a product
>>>that is registered as a viricial disinfectant that the mfgr will state
>>>kills hep A.
>>>
>>>I need the name of a product that is "certifed" for hepatitis (inspector
>>>is not distinguishing among the viruses) to staisfy the municpal health
>>>inspector. We need to get this cafe reopened. Of course I'm away from
>>>my office. Thanks again for any help you can give.
>>>Terry Stimpfel - Montclair State Univ
>>>
>>>Terry
>>>
>>>______________________________________________________
>>>Get Your Private, Free Email at
>>>
>>>
>
>
> ________________________________________________
>__ / _______________________________________________
>_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
>Safety
> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
> \ / U.S.A. RA Member Environmental
>Safety
--============_-1294044243==_============
Content-Type: text/plain; name="EPATuberculocidestext"; charset="us-ascii"
Content-Disposition: attachment; filename="EPATuberculocidestext"
Product: 1 STROKE ENVIRON
EPA Reg: 1043-26
Manuf: CALGON VESTAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
SODIUM 2-BENZYL-4-CHLOROPHENATE 9.400%
SODIUM O-PHENYLPHENATE 11.300%
4-TERT-AMYLPHENOL SODIUM SALT 2.300%
Product: ACTRIL COLD STERILANT
EPA Reg: 52252-7
Manuf: RENAL SYSTEMS, DIV. MINNTECH CORP.,
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
HYDROGEN PEROXIDE 0.800%
PEROXYACETIC ACID 0.060%
Product: ADVANTAGE 128
EPA Reg: 66171-2
Manuf: PRESERVE INTERNATIONAL
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 3.000%
4-TERT-AMYLPHENOL 2.000%
O-PHENYLPHENOL 5.500%
Product: ADVANTAGE 256 CLEANER DISINFECTANT DEODORANT
EPA Reg: 66171-1
Manuf: PRESERVE INTERNATIONAL
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 6.000%
4-TERT-AMYLPHENOL 4.000%
O-PHENYLPHENOL 11.000%
Product: AIRYSOL BRAND MULTI-PURPOSE DISINFECTANT CLEANER
EPA Reg: 33176-6
Manuf: AMREP INC.
Form: AEROSOL
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 0.080%
O-PHENYLPHENOL 0.100%
Product: AIRYSOL BRAND SURFACE DISINFECTANT AND AIR DEODORANT
EPA Reg: 33176-5
Manuf: AMREP INC.
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 44.250%
O-PHENYLPHENOL 0.250%
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 0.330%
Product: ALCIDE EXSPOR 4:1:1-BASE
EPA Reg: 45631-3
Manuf: ALCIDE CORP.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
SODIUM CHLORITE 1.370%
Product: ALCIDE LD 10:1:1-BASE
EPA Reg: 45631-15
Manuf: ALCIDE CORP.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
SODIUM CHLORITE 2.730%
Product: AMPHYL
EPA Reg: 675-43
Manuf: NATIONAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 5.000%
O-PHENYLPHENOL 10.500%
Product: AMPHYL DISINFECTANT DEODORANT DETERGENT
EPA Reg: 675-21
Manuf: NATIONAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
4-TERT-AMYLPHENOL 6.300%
O-PHENYLPHENOL 15.000%
Product: AMPHYL DISINFECTANT DEODORANT SPRAY
EPA Reg: 675-25
Manuf: NATIONAL LABS.
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 78.500%
O-PHENYLPHENOL 0.136%
Product: AMUCHINA
EPA Reg: 65787-1
Manuf: AMUCHINA INTERNATIONAL INC.
Form: SOLUBLE CONCENTRATE
Active Ingredients and Percentages:
SODIUM HYPOCHLORITE 1.020%
Product: ASEPTICARE
EPA Reg: 42964-17
Manuf: AIRKEM PROFESSIONAL PRODUCTS
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 62.740%
ALKYL DIMETHYL ETHYLBENZYL AMMONIUM CHLORIDE 0.150%
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 0.150%
Product: BEAUCOUP
EPA Reg: 303-223
Manuf: HUNTINGTON LABS. INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4 CHLOROPHENOL 4.560%
4 TERT AMYLPHENOL 1.490%
O--PHENYLPHENOL 2.940%
Product: BEAUCOUP GERMICIDAL DETERGENT
EPA Reg: 63281-5
Manuf: RSP PRIVATE LABEL PACKAGING
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 3.490%
4-TERT-AMYLPHENOL 1.920%
O-PHENYLPHENOL 4.280%
Product: BGC-3 GERMCIDAL SYNTHETIC CLEANER
EPA Reg: 875-131
Manuf: DIVERSEY CORP.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
SODIUM 2-BENZYL-4-CHLOROPHENATE 7.920%
SODIUM-O-PHENYLPHENATE 8.850%
4-TERT-AMYLPHENOL, SODIUM SALT 4.100%
Product: BIO-CLEAN
EPA Reg: 51267-1
Manuf: BIOSERV INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 5.000%
ALKYL DIMETHYL ETHYLBENZYL AMMONIUM CHLORIDE 5.000%
BIS (TRIBUTYLTIN) OXIDE 1.000%
Product: BIOSOL
EPA Reg: 777-72
Manuf: L & F PRODUCTS
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 79.000%
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 0.100%
Product: BORAXO DISINFECTANT TILE AND PORCELAIN CLEANER
EPA Reg: 57125-6
Manuf: DIAL CORP.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
ISOPROPANOL 5.000%
5-CHLORO-2-(2,4 DICHLOROPHENOXY) PHENOL 0.070%
Product: BROAD-CIDE I
EPA Reg: 34810-30
Manuf: WEXFORD LABS, INC.
Form: SOLUBLE CONCENTRATE
Active Ingredients and Percentages:
SODIUM 2-BENZYL-4-CHLOROPHENATE 12.480%
SODIUM O-PHENYLPHENATE 13.140%
Product: BROADSPEC 128
EPA Reg: 106-78
Manuf: BRULIN & CO. INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 2.680%
DIDECYL DIMETHYL AMMONIUM CHLORIDE 1.355%
OCTYL DECYL DIMETHYL AMMONIUM CHLORIDE 2.010%
DIOCTYL DIMETHYL AMMONIUM CHLORIDE 1.005%
Product: BULK LYSOL BRAND DISINFECTANT
EPA Reg: 675-19
Manuf: NATIONAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
O-PHENYLPHENOL 2.800%
2-BENZYL-4-CHLOROPHENOL 2.700%
Product: BURNISHINE DISINFECTANT SOLUTION & PUMP SPRAY
EPA Reg: 1130-13
Manuf: BURNISHINE PRODUCTS
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 0.140%
ALKYL DIMETHYL ETHYLBENZYL AMMONIUM CHLORIDE 0.140%
Product: BURNISHINE GERMICIDAL CLOTH
EPA Reg: 9480-5
Manuf: PDI
Form: IMPREGNATED MATERIALS
Active Ingredients and Percentages:
ISOPROPANOL 8.000%
ALKYLDIMETHYLBENZYLAMMONIUM CHLORIDE 0.140%
ALKYLDIMETHYLETHYLBENZYLAMMONIUM CHLORIDE 0.140%
Product: C-SPRA DISINFECTANT DEODORANT
EPA Reg: 334-417
Manuf: HYSAN CORP.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
ETHANOL 66.000%
O PHENYLPHENOL 0.216%
4-TERT-AMYLPHENOL 0.054%
Product: CAVICIDE DISINFECTANT, CLEANER, DEODORANT SOLUTION
EPA Reg: 38526-1
Manuf: MICRO-ASEPTIC PRODUCTS INC.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
ISOPROPANOL 15.300%
DIISOBUTYLPHENOXYETHOXYETHYL DIMETHYL BENZYL AMMONIUM CHL. 0.250%
Product: CEN-O-PHEN DETERGENT GERMICIDE
EPA Reg: 211-26
Manuf: CENTRAL SOLUTIONS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
4-TERT-AMYLPHENOL 4.190%
2-BENZYL-4-CHLOROPHENOL 4.370%
O-PHENYLPHENOL 3.470%
Product: CETYLCIDE-G
EPA Reg: 3150-4
Manuf: CETYLITE INDUSTRIES INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
GLUTARALDEHYDE 46.580%
Product: CHEMI-CAP HOSP-I-SEPTIC-G SURFACE DISINFECTANT DEODORANT
EPA Reg: 7405-51
Manuf: CHEMICAL PACKAGING CORP.
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 53.720%
4-TERT-AMYLPHENOL 0.030%
O-PHENYLPHENOL 0.100%
Product: CIDEX AQUEOUS ACTIVATED DIALDEHYDE SOLUTION
EPA Reg: 7078-1
Manuf: JOHNSON & JOHNSON MEDICAL INC.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
GLUTARALDEHYDE 2.000%
Product: CIDEX FORMULA 7 LONG-LIFE ACTIVATED DIALDEHYDE SOLUTION
EPA Reg: 7078-4
Manuf: JOHNSON & JOHNSON MEDICAL INC.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
GLUTARALDEHYDE 2.000%
Product: CIDEX PLUS 28-DAY SOLUTION
EPA Reg: 7078-14
Manuf: JOHNSON & JOHNSON MEDICAL INC.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
GLUTARALDEHYDE 3.200%
Product: CITRACE HOSPITAL GERMICIDAL DEODORIZER
EPA Reg: 56392-2
Manuf: CALTECH INDUSTRIES INC.
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 66.600%
O-PHENYLPHENOL 0.120%
Product: CLAIRE DISINFECTANT SPRAY
EPA Reg: 706-69
Manuf: CLAIRE MANUFACTURING CO. (REGWEST AGENT)
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 49.950%
4-TERT-AMYLPHENOL 0.045%
O-PHENYLPHENOL 0.176%
Product: CLIDOX-S BASE
EPA Reg: 8714-8
Manuf: PHARMACAL RESEARCH LABS. INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
SODIUM CHLORITE 0.850%
Product: CLIPPERCIDE SPRAY
EPA Reg: 954-10
Manuf: KING RESEARCH INC.
Form: AEROSOL
Active Ingredients and Percentages:
O-PHENYLPHENOL 0.250%
Product: CLOROX
EPA Reg: 5813-1
Manuf: CLOROX CO.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
SODIUM HYPOCHLORITE 5.250%
Product: COLDCIDE 0.25%
EPA Reg: 55195-3
Manuf: COLCIDE INC.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
GLUTARALDEHYDE 0.250%
4-TERT-AMYLPHENOL 0.013%
O-PHENYLPHENOL 0.125%
Product: COLDCIDE-0.25% DISINFECTING WIPES
EPA Reg: 55195-4
Manuf: COLCIDE INC.
Form: IMPREGNATED MATERIALS
Active Ingredients and Percentages:
GLUTARALDEHYDE 0.250%
4-TERT-AMYLPHENOL 0.0025%
O-PHENYLPHENOL 0.0125%
Product: COLDCIDE-10
EPA Reg: 55195-2
Manuf: COLCIDE INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
GLUTARALDEHYDE 10.000%
4-TERT-AMYLPHENOL 0.100%
O-PHENYLPHENOL 0.500%
Product: COLDCIDE-2
EPA Reg: 55195-1
Manuf: COLCIDE INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
GLUTARALDEHYDE 2.000%
4-TERT-AMYLPHENOL 0.020%
O-PHENYLPHENOL 0.100%
Product: CON-O-SYL DISINFECTANT DETERGENT
EPA Reg: 675-27
Manuf: NATIONAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
O-PHENYLPHENOL 11.750%
2-BENZYL-4-CHLOROPHENOL 7.820%
Product: CONCEPT HOSPITAL DISINFECTANT DEODORANT
EPA Reg: 334-214
Manuf: HYSAN CORP.
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 53.000%
4 TERT AMYLPHENOL 0.046%
O-PHENYLPHENOL 0.199%
Product: CRYOCIDE 20
EPA Reg: 10589-4
Manuf: PETTIBONE LAB. INC.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
CHLORINE DIOXIDE 0.720%
DIDECYL DIMETHYL AMMONIUM CHLORIDE 0.400%
Product: DISINFECTANT #2
EPA Reg: 334-317
Manuf: HYSAN CORP.
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 53.460%
O PHENYLPHENOL 0.176%
4-TERT-AMYLPHENOL 0.440%
Product: DISINFECTANT #3
EPA Reg: 334-318
Manuf: HYSAN CORP.
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 53.460%
O PHENYLPHENOL 0.176%
4-TERT-AMYLPHENOL 0.044%
Product: DISINFECTANT #7 HOSPITAL DISINFECTANT DEODORANT
EPA Reg: 334-312
Manuf: HYSAN CORP.
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 53.460%
O PHENYLPHENOL 0.176%
4-TERT-AMYLPHENOL 0.044%
Product: DISPATCH HOSPITAL STAINLESS & PLASTIC CLEANER DISINFECT.
WITH BLEACH
EPA Reg: 56392-7
Manuf: CALTECH INDUSTRIES INC.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
SODIUM HYPOCHLORITE 0.550%
Product: DOW DISINFECTANT FORMULATION 4A
EPA Reg: 67813-3
Manuf: BIOSPAN TECHNOLOGIES, INC.
Form: EMULSIFIABLE CONCENTRATE
Active Ingredients and Percentages:
O-PHENYLPHENOL 0.200%
Product: DOW LIQUID DISINFECTANT FORMULATION 2A
EPA Reg: 67813-1
Manuf: BIOSPAN TECHNOLOGIES, INC.
Form: SOLUBLE CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 5.760%
O-PHENYLPHENOL 6.730%
Product: DUBOIS GSC
EPA Reg: 875-124
Manuf: DIVERSEY CORP.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
SODIUM 2-BENZYL-4-CHLOROPHENATE 5.790%
SODIUM-O-PHENYLPHENATE 3.950%
4-TERT-AMYLPHENOL, SODIUM SALT 0.880%
Product: EN-VIRON D CONCENTRATED PHENOLIC DISINFECTANT
EPA Reg: 1043-14
Manuf: CALGON VESTAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2,2 METHYLENEBIS (3,4,6 TRICHLOROPHENOL) 0.400%
2-BENZYL-4-CHLOROPHENOL 5.000%
4-TERT-AMYLPHENOL 4.500%
O-PHENYLPHENOL 4.600%
Product: EXPOSE PHENOLIC CLEANER
EPA Reg: 4822-109
Manuf: S.C. JOHNSON & SON INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 4.790%
O-PHENYLPHENOL 2.530%
4-TERT-AMYLPHENOL 4.410%
Product: GALAHAD NEUTRAL DETERGENT-GERMICIDE HOSPITAL GRADE
EPA Reg: 541-168
Manuf: PURITAN/CHURCHILL CHEMICAL CORP.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
4-TERT-AMYLPHENOL 5.000%
O PHENYLPHENOL 5.000%
Product: GERM WARFARE CONCENTRATED DETERGENT GERMICIDE
EPA Reg: 52-208
Manuf: WEST CHEMICAL PRODUCTS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
SODIUM 2-BENZYL-4-CHLOROPHENATE 4.200%
SODIUM-O-PHENYLPHENATE 16.900%
4-TERT-AMYLPHENOL, SODIUM SALT 2.700%
Product: GLD GERMICIDIAL LIQUID DETERGENT
EPA Reg: 2311-4
Manuf: HAAG LABS. INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 4.650%
Product: HERPICIDE SPRAY
EPA Reg: 48920-1
Manuf: JABCO MANUFACTURING INC.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
ISOPROPANOL 40.000%
ALKYLDIMETHYLBENZYLAMMONIUM CHLORIDE 2.00%
Product: HOSPITAL BROAD BAND
EPA Reg: 49403-6
Manuf: NIPA LABS. INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 5.000%
4-TERT-AMYLPHENOL 1.250%
O-PHENYLPHENOL 4.250%
Product: HOSPITAL DISINFECTANTS
EPA Reg: 4000-42
Manuf: SOUTHERN CHEMICAL PRODUCTS CO.
Form: AEROSOL
Active Ingredients and Percentages:
ISOPROPANOL 67.500%
O-PHENYLPHENOL 0.150%
Product: HOSPITAL FOAM CLEANER DISINFECTANT
EPA Reg: 56392-5
Manuf: CALTECH INDUSTRIES INC.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
O-PHENYLPHENOL 0.370%
Product: HOSPITAL SURFACE DISINFECTANT ROOM DEODORIZER
EPA Reg: 3862-104
Manuf: ABC COMPOUNDING CO. INC.
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 53.460%
4-TERT-AMYLPHENOL 0.020%
O-PHENYLPHENOL 0.080%
Product: IONOX DISINFECTANT CLEANER
EPA Reg: 2296-93
Manuf: NATIONAL CHEMICAL LAB. OF PA INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
NONYLPHENOXYPOLYETHOXYETHANOL IODINE COMPLEX 1.750%
Product: ISOTEX 70 DISINFECTING TOWELETTES
EPA Reg: 10492-4
Manuf: PALMERO HEALTH CARE
Form: IMPREGNATED-MATERIALS
Active Ingredients and Percentages:
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 0.120%
ISOPROPANOL 63.250%
ALKYLDIMETHYLETHYLBENZYLAMMONIUM CHLORIDE 0.120%
Product: KLEENASEPTIC GERMICIDAL SURFACE CLEANSER
EPA Reg: 38526-3
Manuf: MICRO ASEPTIC PRODUCTS INC.
Form: PRESSURIZED LIQUID
Active Ingredients and Percentages:
ISOPROPANOL 14.850%
DIISOBUTYLPHENOXYETHOXYETHYLDIMETHYLBENZYLAMMONIUM CHLORIDE .250%
Product: LEGPHENE GERMICIDAL CLEANER
EPA Reg: 2212-5
Manuf: WALTER G. LEGGE CO. INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
POTASSIUM 2 BENZYL 4 CHLOROPHENATE 4.000%
O-PHENYLPHENOL, POTASSIUM SALT 2.400%
4-TERT-AMYLPHENOL, POTASSIUM SALT 3.800%
Product: LEHN & FINK INSTRUMENT GERMICIDE
EPA Reg: 675-15
Manuf: NATIONAL LABS.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 0.250%
O PHENYLPHENOL 0.518%
ISOPROPANOL 0.083%
Product: LPHAG
EPA Reg: 1043-91
Manuf: CALGON VESTAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
4-TERT-AMYLPHENOL 7.400%
O-PHENYLPHENOL 7.300%
Product: LPHSE
EPA Reg: 1043-92
Manuf: CALGON VESTAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
4-TERT-AMYLPHENOL 7.400%
O-PHENYLPHENOL 7.300%
Product: LYSOL BRAND DISINFECTANT SPRAY
EPA Reg: 777-53
Manuf: L & F PRODUCTS
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 79.000%
O-PHENYLPHENOL 0.100%
Product: LYSOL BRAND DISINFECTANT TRIGGER SPRAY
EPA Reg: 777-73
Manuf: L & F PRODUCTS
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
O-PHENYLPHENOL 0.070%
Product: LYSOL BRAND FOAMING DISINFECTANT BASIN, TUB, & TILE
CLEANER
EPA Reg: 777-71
Manuf: L & F PRODUCTS
Form: AEROSOL
Active Ingredients and Percentages:
DIDECYL DIMETHYL AMMONIUM CHLORIDE 0.025%
OCTYL DECYL DIMETHYL AMMONIUM CHLORIDE 0.050%
DIOCTYL DIMETHYL AMMONIUM CHLORIDE 0.025%
Product: M-2205 DISINFECTANT/SANITIZER
EPA Reg: 47371-26
Manuf: H & S CHEMICALS DIVISION
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
IODINE 1.750%
Product: M-2205 FARM DISINFECTANT/SANITIZER
EPA Reg: 47371-93
Manuf: H & S CHEMICALS DIV.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
POLYETHOXYPOLYPROPOXYETHANOL IODINE COMPLEX 1.750%
Product: MATAR GERMICIDAL DETERGENT
EPA Reg: 63281-4
Manuf: RSP PRIVATE LABEL PACKAGING
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
O-PHENYLPHENOL 5.800%
2-BENZYL-4-CHLOROPHENOL 9.120%
4-TERT-AMYLPHENOL 2.970%
Product: MATAR II
EPA Reg: 303-225
Manuf: HUNTINGTON LABS. INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 9.120%
4 TERT AMYLPHENOL 2.970%
O-PHENYLPHENOL 5.880%
Product: MEDAPHENE PLUS DISINFECTANT DEODORANT
EPA Reg: 11694-98
Manuf: DYMON INC.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
ETHANOL 68.000%
O-PHENYLPHENOL 0.190%
Product: MEDAPHENE PLUS DISINFECTANT SPRAY
EPA Reg: 11694-99
Manuf: DYMON INC.
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 68.000%
O-PHENYLPHENOL 0.190%
Product: METRICIDE 28 LONG-LIFE ACTIVATED DIALDEHYDE SOLUTION
EPA Reg: 46781-2
Manuf: METREX RESEARCH CORP.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
GLUTARALDEHYDE 2.000%
Product: METRICIDE ACTIVATED DIALDEHYDE SOLUTION
EPA Reg: 46781-1
Manuf: METREX RESEARCH CORP.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
GLUTARALDEHYDE 2.000%
Product: METRICIDE PLUS 30
EPA Reg: 46781-4
Manuf: METREX RESEARCH CORP.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
GLUTARALDEHYDE 3.200%
Product: MIKROKLENE
EPA Reg: 1677-22
Manuf: ECOLAB INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
BUTOXYPOLYPROPOXYPOLYETHOXYETHANOL IODINE COMPLEX 1.750%
PHOSPHORIC ACID 6.500%
Product: MINTOL-128 FRAGRANT MINT DISINFECTANT
EPA Reg: 1057-61
Manuf: C.B. DOLGE CO.
Form: EMULSIFIABLE CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 3.000%
4-TERT-AMYLPHENOL 2.000%
O-PHENYLPHENOL 5.000%
Product: MIS-TERY HOUSEHOLD DISINFECTANT AND DEODORIZER SPRAY
EPA Reg: 334-385
Manuf: HYSAN CORP.
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 53.460%
4 TERT AMYLPHENOL 0.044%
O-PHENYLPHENOL 0.176%
Product: MISTY HOSPITAL III DISINFECTANT AND DEODORANT
EPA Reg: 10807-178
Manuf: AMREP, INC.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
ETHANOL 67.00%
4-TERT-AMYLPHENOL 0.045%
O-PHENYLPHENOL 0.177%
Product: MISTY II DISINFECTANT & DEODORANT
EPA Reg: 10807-177
Manuf: AMREP, INC.
Form: PRESSURIZED LIQUID
Active Ingredients and Percentages:
ETHANOL 61.348%
4-TERT-AMYLPHENOL 0.045%
O-PHENYLPHENOL 0.177%
Product: MTP PHENOLIC GERMICIDAL DETERGENT
EPA Reg: 47371-163
Manuf: H & S CHEMICALS DIV.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 9.120%
4-TERT-AMYLPHENOL 2.970%
O-PHENYLPHENOL 5.880%
SODIUM LAURYL SULFATE 5.240%
Product: MULTI-WASH
EPA Reg: 61181-1
Manuf: MULTI-WASH MEDICAL LTD.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
NONYLPHENOXYPOLYETHOXYETHANOL IODINE COMPLEX 1.750%
PHOSPHORIC ACID 6.800%
Product: MULTI-WASH MINI
EPA Reg: 61181-2
Manuf: MULTI-WASH MEDICAL, LTD.
Form: SOLUBLE CONCENTRATE
Active Ingredients and Percentages:
NONYLPHENOXYPOLYETHOXYETHANOL - IODINE COMPLEX 1.750%
PHOSPHORIC ACID 6.800%
Product: NEW-O-SYL DISINFECTANT DETERGENT
EPA Reg: 675-24
Manuf: NATIONAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 4.500%
4-TERT-AMYLPHENOL 1.000%
O-PHENYLPHENOL 5.000%
Product: NEW-O-SYL DISINFECTANT-DETERGENT
EPA Reg: 675-46
Manuf: NATIONAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 7.240%
O-PHENYLPHENOL 2.230%
Product: OCIDE HOSPITAL CLEANER-DISINFECTANT
EPA Reg: 49403-23
Manuf: NIPA LABS. INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
POTASSIUM 2-BENZYL-4-CHLOROPHENATE 10.100%
O-PHENYLPHENOL, POTASSIUM SALT 4.900%
4-TERT-AMYLPHENOL, POTASSIUM SALT 2.500%
Product: OMC II READY TO USE
EPA Reg: 46851-5
Manuf: PROCHEM CO.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 0.030%
O-PHENYLPHENOL 0.280%
Product: OMNI-II
EPA Reg: 46851-1
Manuf: PROCHEM CO.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 1.000%
O-PHENYLPHENOL 9.000%
Product: OMNICIDE 14
EPA Reg: 46851-4
Manuf: PROCHEM CO.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
GLUTARALDEHYDE 2.000%
Product: OMNICIDE PLUS
EPA Reg: 46851-9
Manuf: PROCHEM CO.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
GLUTARALDEHYDE 3.400%
Product: OMNICIDE STERILIZING AND DISINFECTING SOLUTION
EPA Reg: 46851-2
Manuf: PROCHEM CO.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
GLUTARALDEHYDE 2.000%
Product: ONYX DETERGENT DISINFECTANT PUMP SPRAY
EPA Reg: 1839-83
Manuf: ONYX CHEMCIAL CO.
Form: SPRAY READY TO USE
Active Ingredients and Percentages:
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 0.105%
ALKYL DIMETHYL ETHYLBENZYL AMMONIUM CHLORIDE 0.105%
Product: OPTI-PHENE
EPA Reg: 11725-9
Manuf: BIO-TEK INDUSTRIES INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
4-TERT-AMYLPHENOL 5.250%
2-BENZYL-4-CHLOROPHENOL 3.000%
O-PHENYLPHENOL 3.000%
Product: OXONIA ACTIVE
EPA Reg: 1677-129
Manuf: ECOLAB INC.
Form: SOLUBLE CONCENTRATE
Active Ingredients and Percentages:
HYDROGEN PEROXIDE 27.50%
PEROXYACETIC ACID 5.800%
Product: P/P DISINFECTANT DEODORANT SPRAY 'G'
EPA Reg: 11525-30
Manuf: CCL CUSTOM MANUFACTURING INC.
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 53.088%
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 0.072%
ALKYL DIMETHYL ETHYLBENZYL AMMONIUM CHLORIDE 0.072%
Product: PD-64 PHENOLIC BASE CLEANER & DISINFECTANT
EPA Reg: 5741-6
Manuf: SPARTAN CHEMICAL CO.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
O-PHENYLPHENOL 6.130%
Product: PERMACIDE BRAND (RISTEX) GERMICIDAL DISINFECTANT
EPA Reg: 8383-3
Manuf: SPORICIDIN INTERNATIONAL
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
BORAX 0.470%
PHENOL 1.410%
SODIUM PHENATE 0.240%
Product: PERMACIDE BRAND RISTEX GERMICIDAL/DISINFECTANT SPRAY
EPA Reg: 8383-4
Manuf: SPORICIDIN INTERNATIONAL
Form: AEROSOL
Active Ingredients and Percentages:
SODIUM PHENATE 0.240%
PHENOL 1.410%
ETHANOL 18.000%
Product: PHENEX CEL
EPA Reg: 34810-16
Manuf: WEXFORD LABS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
SODIUM 2-BENZYL-4-CHLOROPHENATE 7.150%
SODIUM O-PHENYLPHENATE 8.450%
Product: PHENO CEN
EPA Reg: 211-25
Manuf: CENTRAL SOLUTIONS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
POTASSIUM 2 BENZYL 4 CHLOROPHENATE 8.030%
O-PHENYLPHENOL, POTASSIUM SALT 6.280%
4-TERT-AMYLPHENOL, POTASSIUM SALT 4.300%
Product: PHENO-CEN SPRAY DISINFECTANT DEODORANT
EPA Reg: 211-32
Manuf: CENTRAL SOLUTIONS INC.
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 69.623%
O-PHENYLPHENOL 0.210%
Product: PHENOCIDE 128
EPA Reg: 63281-8
Manuf: RSP PRIVATE LABEL PACKAGING
Form: SOLUBLE CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 4.750%
O-PHENYLPHENOL 4.750%
Product: PHENOCIDE 256
EPA Reg: 63281-7
Manuf: RSP PRIVATE LABEL PACKAGING
Form: SOLUBLE CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 9.500%
O-PHENYLPHENOL 9.500%
Product: PHENOSOL
EPA Reg: 63163-1
Manuf: KC LABS.
Form: SOLUBLE CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 1.000%
O-PHENYLPHENOL 9.000%
Product: PRECISE HOSPITAL FOAM CLEANER DISINFECTANT
EPA Reg: 56392-1
Manuf: CALTECH INDUSTRIES INC.
Form: AEROSOL
Active Ingredients and Percentages:
O PHENYLPHENOL 0.370%
Product: PREMEASURED TERGISYL DISINFECTANT DETERGENT
EPA Reg: 675-16
Manuf: NATIONAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
POTASSIUM 2 BENZYL 4 CHLOROPHENATE 7.450%
Product: PROCESS VESPHENE II
EPA Reg: 1043-115
Manuf: CONVATEC
Form: SOLUBLE CONCENTRATE
Active Ingredients and Percentages:
P-TERT-AMYLPHENOL 8.780%
O-PHENYLPHENOL 10.160%
Product: PURITAN #6790 DETERGENT-GERMICIDE
EPA Reg: 541-265
Manuf: PURITAN/CHURCHILL CHEMICAL CORP.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
4-TERT-AMYLPHENOL 7.350%
O PHENYLPHENOL 2.910%
TETRASODIUM ETHYLENEDIAMINETETRAACETATE 2.780%
Product: READY TO USE THYMO-CIDE
EPA Reg: 34810-25
Manuf: WEXFORD LABS INC.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
THYMOXYDICHLOROACETIC ACID 0.051%
Product: READY TO USE TOPPS
EPA Reg: 34810-22
Manuf: WEXFORD LABS INC.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
O-PHENYLPHENOL 0.027%
THYMOL 0.027%
Product: READY TO USE WEX-CIDE
EPA Reg: 34810-21
Manuf: WEXFORD LABS INC.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 0.023%
O-PHENYLPHENOL 0.260%
Product: RENALIN COLD STERILANT FOR DIALYZER USE
EPA Reg: 52252-5
Manuf: RENAL SYSTEMS, DIV. MINNTECH CORP.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
HYDROGEN PEROXIDE 20.000%
PEROXYACETIC ACID 4.000%
Product: RENALIN COLD STERILANT FOR USE WITH RENATRON DIALYZER REPROC
EPA Reg: 52252-6
Manuf: RENAL SYSTEMS, DIV. MINNTECH CORP.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
HYDROGEN PEROXIDE 20.000%
PEROXYACETIC ACID 4.000%
Product: RTU PHENOLIC GERMICIDAL DETERGENT
EPA Reg: 63281-1
Manuf: RSP PRIVATE LABEL PACKAGING
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 0.105%
4-TERT-AMYLPHENOL 0.035%
O-PHENYLPHENOL 0.070%
Product: SAN PHENO X DISINFECTANT AND DEODORANT
EPA Reg: 303-26
Manuf: HUNTINGTON LABS. INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4 CHLOROPHENOL 6.180%
Product: SANI-CLOTH GERMICIDAL WIPES
EPA Reg: 9480-4
Manuf: PDI
Form: IMPREGNATED MATERIALS
Active Ingredients and Percentages:
ISOPROPANOL 55.00%
ALKYLDIMETHYLBENZYLAMMONIUM CHLORIDE 0.250%
ALKYLDIMETHYLETHYLBENZYLAMMONIUM CHLORIDE 0.250%
Product: SINGLE PHENOLIC HOSPITAL DISINFECTANT
EPA Reg: 4313-66
Manuf: CARROLL CO.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 9.720%
Product: SPOR-O-SYL
EPA Reg: 675-39
Manuf: NATIONAL LABS.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
HYDROGEN PEROXIDE 6.000%
PHOSPHORIC ACID 0.850%
Product: SPORICIDIN BRAND DISINFECTANT TOWELETTE
EPA Reg: 8383-7
Manuf: SPORICIDIN INTERNATIONAL
Form: IMPREGNATED MATERIALS
Active Ingredients and Percentages:
SODIUM PHENATE 0.060%
PHENOL 1.560%
Product: SPRAY DISINFECTANT
EPA Reg: 67603-4
Manuf: SHERWIN-WILLIAMS DIVERSIFIED BRANDS INC.
Form: PRESSURIZED LIQUID
Active Ingredients and Percentages:
ETHANOL 37.000%
ALKYLDIMETHYLBENZYLAMMONIUM CHLORIDE 0.050%
ALKYLDIMETHYLETHYLBENZYLAMMONIUM CHLORIDE 0.050%
Product: STAPHENE DISINFECTANT SPRAY AND DEODORIZER
EPA Reg: 1043-19
Manuf: CALGON VESTAL LABS.
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 53.096%
2-BENZYL-4-CHLOROPHENOL 0.077%
4-TERT-AMYLPHENOL 0.074%
O-PHENYLPHENOL 0.041%
Product: STAT-5 ANTIMICROBIAL SPRAY
EPA Reg: 69264-1
Manuf: MCCORMICK DISTILLING CO. INC.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
ETHANOL 64.80%
ALKYLDIMETHYLBENZYLAMMONIUM CHLORIDE 0.080%
Product: STERIL-IZE
EPA Reg: 63281-6
Manuf: RSP PRIVATE LABEL PACKAGING
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
GLUTARALDEHYDE 2.000%
Product: STERIPHENE II BRAND DISINFECTANT DEODORANT
EPA Reg: 5741-22
Manuf: SPARTAN CHEMICAL CO.
Form: AEROSOL
Active Ingredients and Percentages:
ETHANOL 64.000%
2-BENZYL-4-CHLOROPHENOL 0.071%
O-PHENYLPHENOL 0.051%
Product: STERIS 20-D
EPA Reg: 58779-2
Manuf: STERIS CORP.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
SODIUM PERBORATE MONOHYDRATE 30.000%
ACETYLSALICYLIC ACID 30.000%
Product: SUPER WEX-CEL CONCENTRATED GERMICIDAL DETERGENT
EPA Reg: 34810-7
Manuf: WEXFORD LABS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
SODIUM O-PHENYLPHENATE 13.270%
SODIUM 2-BENZYL-4-CHLOROPHENATE 13.020%
Product: T.B.Q. GERMICIDAL DETERGENT
EPA Reg: 1043-60
Manuf: CALGON VESTAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 8.000%
Product: TAMED IODINE WESCODYNE
EPA Reg: 52-150
Manuf: WEST CHEMICAL PRODUCTS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
POLYETHOXYPOLYPROPOXYPOLYETHOXYETHANOL IODINE COMPLEX 9.100%
NONYLPHENOXYPOLYETHOXYETHANOL IODINE COMPLEX 8.740%
Product: TAMED IODINE WESCODYNE G
EPA Reg: 52-111
Manuf: WEST CHEMICAL PRODUCTS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
POLYETHOXYPOLYPROPOXYPOLYETHOXYETHANOL IODINE COMPLEX 9.100%
NONYLPHENOXYPOLYETHOXYETHANOL IODINE COMPLEX 8.740%
HYDROGEN CHLORIDE 0.110%
Product: TEK-PHENE CLEANER-DISINFECTANT-
DEODORANT
EPA Reg: 11725-8
Manuf: BIO-TEK INDUSTRIES INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 6.500%
4-TERT-AMYLPHENOL 10.000%
O-PHENYLPHENOL 6.000%
Product: TEK-TROL DISINFECTANT-CLEANER CONCENTRATE
EPA Reg: 11725-7
Manuf: BIO-TEK INDUSTRIES INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 10.000%
4-TERT-AMYLPHENOL 4.000%
O-PHENYLPHENOL 12.000%
Product: TERGISYL HOSPITAL TYPE DETERGENT DISINFECTANT
EPA Reg: 675-17
Manuf: NATIONAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
4-TERT-AMYLPHENOL 1.800%
O-PHENYLPHENOL 5.700%
Product: THYMO-CIDE
EPA Reg: 34810-18
Manuf: WEXFORD LABS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
THYMOL 13.000%
Product: TOPPS
EPA Reg: 34810-19
Manuf: WEXFORD LABS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
O-PHENYLPHENOL 7.000%
THYMOL 7.000%
Product: TRI-CEN GERMICIDAL DETERGENT
EPA Reg: 211-36
Manuf: CENTRAL SOLUTIONS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
SODIUM 2-BENZYL-4-CHLOROPHENATE 4.400%
SODIUM O PHENYLPHENATE 2.820%
4-TERT-AMYLPHENOL, SODIUM SALT 2.490%
Product: ULTRA
EPA Reg: 34810-28
Manuf: WEXFORD LABS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 8.640%
O-PHENYLPHENOL 10.000%
Product: VANI-SOL BOWL CLEANSE
EPA Reg: 675-1
Manuf: NATIONAL LABS.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
HYDROGEN CHLORIDE 23.000%
2 BENZYL 4 CHLOROPHENOL 0.010%
ALKYL DIMETHYL BENZYL AMMONIUM CHLORIDE 0.050%
Product: VESPHENE II SE
EPA Reg: 1043-87
Manuf: CALGON VESTAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
SODIUM O-PHENYLPHENATE 9.650%
4-TERT-AMYLPHENOL, SODIUM SALT 8.340%
Product: VESTA-SYDE INTERIM INSTRUMENT DECONTAMINATION SOLUTION
EPA Reg: 1043-114
Manuf: CALGON VESTAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
4-TERT-AMYLPHENOL 7.360%
O-PHENYLPHENOL 8.550%
Product: VESTAL AMERSE INSTRUMENT GERMICIDE
EPA Reg: 1043-36
Manuf: CALGON VESTAL LABS.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 5.250%
O-PHENYLPHENOL 1.000%
Product: VIRAHOL
EPA Reg: 60142-1
Manuf: VERIDIEN CORP.
Form: LIQUID READY TO USE
Active Ingredients and Percentages:
ISOPROPANOL 70.000%
Product: WAVICIDE-01
EPA Reg: 15136-1
Manuf: WAVE ENERGY SYSTEMS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
GLUTARALDEHYDE 2.000%
Product: WAVICIDE-01 CONCENTRATE
EPA Reg: 15136-2
Manuf: WAVE ENERGY SYSTEMS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
GLUTARALDEHYDE 20.500%
Product: WAVICIDE-06
EPA Reg: 15136-6
Manuf: WAVE ENERGY SYSTEMS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
GLUTARALDEHYDE 0.250%
Product: WEX-CEL CONCENTRATED GERMICIDAL DETERGENT
EPA Reg: 34810-6
Manuf: WEXFORD LABS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
SODIUM O-PHENYLPHENATE 6.490%
SODIUM 2-BENZYL-4-CHLOROPHENATE 6.500%
Product: WEX-CIDE CONCENTRATED GERMICIDAL DETERGENT
EPA Reg: 34810-8
Manuf: WEXFORD LABS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 5.760%
O-PHENYLPHENOL 6.730%
Product: WEXFORD DRI-CIDE
EPA Reg: 34810-17
Manuf: WEXFORD LABS INC.
Form: POWDER CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 6.090%
O-PHENYLPHENOL 7.330%
Product: WINTER-PHENE
EPA Reg: 421-16
Manuf: JAMES VARLEY & SONS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
ESSENTIAL OILS 0.500%
ISOPROPANOL 25.940%
2-BENZYL-4-CHLOROPHENOL 4.900%
O-PHENYLPHENOL 0.750%
Product: WIPE OUT
EPA Reg: 58994-1
Manuf: HEALTH CARE PRODUCTS INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
GLUTARALDEHYDE 0.300%
PHENOL 1.000%
Product: XXX DISINFECTANT
EPA Reg: 4959-15
Manuf: WEST AGRO INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
NONYLPHENOXYPOLYETHOXYETHANOL IODINE COMPLEX 1.750%
Product: YYY DISINFECTANT
EPA Reg: 4959-34
Manuf: WEST AGRO INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
NONYLPHENOXYPOLYETHOXYETHANOL IODINE COMPLEX 0.350%
Product: ZEP FORMULA 165-A
EPA Reg: 1270-193
Manuf: ZEP MANUFACTURING CO.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
4-TERT-AMYLPHENOL, POTASSIUM SALT 2.710%
O-PHENYLPHENOL, POTASSIUM SALT 4.160%
POTASSIUM-2-BENZYL-4-CHLOROPHENATE 6.430%
Product: ZEP FORMULA 3387
EPA Reg: 1270-238
Manuf: ZEP MANUFACTURING CO.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
2-BENZYL-4-CHLOROPHENOL 5.760%
O-PHENYLPHENOL 6.730%
Product: ZZZ DISINFECTANT
EPA Reg: 4959-16
Manuf: WEST AGRO INC.
Form: LIQUID CONCENTRATE
Active Ingredients and Percentages:
NONYLPHENOXYPOLYETHOXYETHANOL IODINE COMPLEX 1.750%
EPA LIST OF APPORVED TUBERCULOCIDAL DISINFECTANTS AS OF 5/15/97
--============_-1294044243==_============
Content-Type: text/plain; charset="us-ascii"
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
--============_-1294044243==_============--
=========================================================================
Date: Wed, 3 Feb 1999 16:32:53 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Anderson, Bruce"
Subject: Western Canada Biosafety Symposium
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Announcing:
The 2nd Annual
Western Canada Biosafety Symposium
Presented by:
BC Centre for Disease Control, The Provincial Laboratory
The Department of Health Safety and Environment, UBC
ABSA - Canada
June 7th and 8th, 1999
The University of British Columbia
Vancouver, BC Canada
For further Information and Registration visit the Symposium Website:
Agenda Highlights:
Bioterrorism - How Real is the Threat? - Stefan Wagener
Importing Human and Animal Pathogens - Maureen Best
Animal Biosafety Techniques - Scott Rusk
Laboratory Acquired Infections and Control Mechanisms - Jonathan Richmond
Are You Prepared for the New and Emerging Viruses? - Darrel Cook
The Real Hot Zone: High Containment in Canada - Paul Langevin
WCB - The New Biohazard Regulations - Alan Brose
Disposal Options for Biomedical Waste - Ed Krisiunas
Transportation of Dangerous Goods Regulation 6.2 - Update - Eric Cook
Testing Compliance to TDG 6.2: A Study - Michael A. Noble
And of course the "World Famous" West Coast Salmon Barbeque at the UBC
Museum of Anthropology
Hope to see you all there.
> Bruce
>
> T. Bruce Anderson
> Biosafety Officer
> * anderson@safety.ubc.ca
> * safety.ubc.ca
> * (604) 822-7596
> * (604) 880-0711 cellular
>
=========================================================================
Date: Thu, 4 Feb 1999 13:46:53 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: LS&EM West Coast Seminar
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Make Plans NOW to Attend:
"Current Environmental Management Topics
for
Research & Industrial Laboratories"
A One Day Seminar for Laboratory Managers
Thursday, May 20, 1999
Diamond Bar, California
Hosted by Laboratory Safety & Environmental Management - The Conference
(LS&EM)
Sponsored by the South Coast Air Quality Management District and PRIZIM Inc.
For more information, visit the LS&EM web site at
LSEMCONFERE
NCE.htm.
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
=========================================================================
Date: Thu, 4 Feb 1999 15:33:50 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Anderson, Bruce"
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Okay, so we now know some of you are unable to view "frames" on web pages.
If you had trouble viewing the Symposium Website please try it again. We've
re-done the site using a "frameless" approach.
This has actually worked out okay, in that it has given us a second
opportunity to advertise the event:
Announcing (for a second time):
The 2nd Annual
Western Canada Biosafety Symposium
Presented by:
BC Centre for Disease Control, The Provincial Laboratory
The Department of Health Safety and Environment, UBC
ABSA - Canada
June 7th and 8th, 1999
The University of British Columbia
Vancouver, BC Canada
For further Information and Registration visit the Symposium Website:
Agenda Highlights:
Bioterrorism - How Real is the Threat? - Stefan Wagener
Importing Human and Animal Pathogens - Maureen Best
Animal Biosafety Techniques - Scott Rusk
Laboratory Acquired Infections and Control Mechanisms - Jonathan Richmond
Are You Prepared for the New and Emerging Viruses? - Darrel Cook
The Real Hot Zone: High Containment in Canada - Paul Langevin
WCB - The New Biohazard Regulations - Alan Brose
Disposal Options for Biomedical Waste - Ed Krisiunas
Transportation of Dangerous Goods Regulation 6.2 - Update - Eric Cook
Testing Compliance to TDG 6.2: A Study - Michael A. Noble
And of course the "World Famous" West Coast Salmon Barbeque at the UBC
Museum of Anthropology
Hope to see you all there.
> Bruce
>
> T. Bruce Anderson
> Biosafety Officer
> * anderson@safety.ubc.ca
> * safety.ubc.ca
> * (604) 822-7596
> * (604) 880-0711 cellular
>
=========================================================================
Date: Fri, 5 Feb 1999 15:27:53 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Betty Kupskay
Subject: Institutional Biosafety Committee
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Betty Kupskay
02/05/99 04:27 PM
We are presently drafting the terms of reference for our institutional
biosafety committee and have had some points of contention with members
with regard to employee representation and voting rights.
Would anyone out there care to share the format of the terms of reference
of their IBC? This would be greatly appreciated.
Thanks in advance!
Betty Kupskay
Biosafety Specialist/Health Canada
Federal Laboratories
1015 Arlington St., Suite A1010
Winnipeg, MB
R3E 3P6
ph:204-789-2065
FAX: 204-789-2069
e:mail: betty_kupskay@hc-sc.gc.ca
=========================================================================
Date: Sat, 6 Feb 1999 10:08:04 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ben Owens
Subject: USE OF NITRILE GLOVES
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Are thin (4 mil) nitrile gloves (such as the Best N-Dex or Ansell Edmont
TNT) recognized as providing protection against bloodborne pathogens and
other biological hazards (bacteria and viruses)? These nitrile gloves
provide dexterity similar to latex exam gloves but offer increased
chemical resistance and also get away from potential latex allergy
problems. I have yet to see a real recommendation for use of the
nitrile gloves for protection against BBPs. NIOSH sent me many, many
pages of information on latex allergies but no where did they recommend
changing glove types (to nitrile or any other glove material). I'm just
wondering if others are using the nitrile gloves for biological hazards,
and if not, are there limitations to the nitrile gloves that I am not
aware of? Thanks. Ben
--
Ben Owens, Chemical Hygiene Officer
University of Nevada, Reno
Environmental Health and Safety Department, MS 328
Reno, NV 89557
(702) 327-5196
(702) 784-4553 fax
=========================================================================
Date: Sun, 7 Feb 1999 23:25:38 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Mike Miller
Subject: Re: USE OF NITRILE GLOVES
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
Nitrile gloves offer very good protection against bio-infectious materials,
however, I worry about your comment that nitrile gloves offer increased
chemical resistance. Nitrile gloves, like many other types of single and
combination type gloves offer limited protection to many different types of
chemicals. They also offer excellent protection against some chemicals, but
this is not an across the board comment, especially for 4 mil nitrile gloves.
You must always check the manufacturer's permeation data for that specific
model and thickness glove, along with the work practice of the person wearing
the gloves (splash protection or immersion protection).
Michael E. Miller
Industrial Hygiene and Safety Manager
Occupational Health and Safety Program
FBI, Laboratory
Washington, DC 20535
202 324 1675
=========================================================================
Date: Mon, 8 Feb 1999 09:23:44 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: Free Lab Safety Training for Students
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
The Northeastern Section of the American Chemical Society is
sponsoring a series of free, one-day lab safety training seminars for
undergraduates and graduate students.
The next seminar will be presented this coming Saturday,
February 13th at Boston College in the Shea Conference
Room of the Conte Forum.
The seminar, training materials, refreshments, and lunch will be
provided without charge. Registration/Check-in is at 8:30AM with
the seminar lasting until about 4PM.
Please encourage students to attend. This is a students only
seminar.
After a pilot program at five institutions confirmed the effectiveness
of single, large dose safety training programs for students (in
addition to regular pre-lab briefings), LSW has been offering these
one-day student seminars at colleges and universities throughout
the United States. Pilot sites included WPI, Marine Biology
Laboratory, Duquesne/Carnegie Mellon, Villanova, and University
of Nevada-Reno.
Other participating institutions include University of Massachusetts
at Dartmouth, Framingham State College, and Simmons College.
For more information about the Boston College seminar or hosting
the seminar at your institution, please contact Tricia McGann,
Membership Services Manager at LSW (lswpfm@).
Regards, ...... jim
*****************************************************
James A. Kaufman, President
The Laboratory Safety Workshop
Safety in Science Education
192 Worcester Road, Natick, MA 01760
508-647-1900 Fax: 508-647-0062 labsafe@
LSW is a national non-profit educational organization dedicated to making
health and safety an integral and important part of science education. Free
copies of our Laboratory Safety Guidelines, seminar schedule, Publications
List, AV-Lending Library List, and membership information are available on
request. The LABSAFETY-L discussion list is a public service of LSW.
******************************************************.
=========================================================================
Date: Mon, 8 Feb 1999 09:54:05 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: USE OF NITRILE GLOVES
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
I do not believe that the 4 mil glove is rated for chemical resistance. 6
& 8 mil are rated.
bob
>Are thin (4 mil) nitrile gloves (such as the Best N-Dex or Ansell Edmont
>TNT) recognized as providing protection against bloodborne pathogens and
>other biological hazards (bacteria and viruses)? These nitrile gloves
>provide dexterity similar to latex exam gloves but offer increased
>chemical resistance and also get away from potential latex allergy
>problems. I have yet to see a real recommendation for use of the
>nitrile gloves for protection against BBPs. NIOSH sent me many, many
>pages of information on latex allergies but no where did they recommend
>changing glove types (to nitrile or any other glove material). I'm just
>wondering if others are using the nitrile gloves for biological hazards,
>and if not, are there limitations to the nitrile gloves that I am not
>aware of? Thanks. Ben
>--
>Ben Owens, Chemical Hygiene Officer
>University of Nevada, Reno
>Environmental Health and Safety Department, MS 328
>Reno, NV 89557
>(702) 327-5196
>(702) 784-4553 fax
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Mon, 8 Feb 1999 10:14:29 EST
Reply-To: Karen=B.=Byers%SS%DFCI@EYE.DFCI.HARVARD.EDU
Sender: A Biosafety Discussion List
From: "Karen B. Byers"
Subject: Re: USE OF NITRILE GLOVES
MIME-Version: 1.0
Content-type: text/plain; charset=us-ascii
I agree the chemical resistance of nitrile makes it very helpful for
biohazard/chem hazardous protocols. The N-DEX 6 mils, 11"long, hand specific
"non-Latex procedures gloves" has the biohazard symbol in a shield on it, and
states "This glove meets the emergency medical glove requirements of NFPA
1999, Standard protective clothing for emergency medical operations, 1997
edition" This description on the box was the first I'd heard of this
standard, so I don't actually know what other glove products meet it; maybe
someone else does?
Karen Byers, RBP, CBSP
Dana-Farber Cancer Institute
44 Binney Street Boston, MA 02115
karen_byers@dfci.harvard.edu
=========================================================================
Date: Mon, 8 Feb 1999 09:40:03 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Fay Schmaltz
Subject: Fire response in BL3
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hello,
I am looking for information on fire response policies in other BL3
facilities. Specifically, who responds to a fire within BL3 ( i.e.
municipal firefighting personnel, or other)? Do firefighting staff enter
the facility, or is it designated as a burnout zone? Are there any special
entry requirements such as vaccination or specialized training if the
firefighting staff enter the area? How is decontamination of equipment
and protective gear handled? Any information you could provide on your
facility would be greatly appreciated.
Thanks in advance,
Fay
==================================================================
Fay Schmaltz
Biosafety Officer
Medical Countermeasures Section
Defence Research Establishment Suffield
Box 4000 Stn Main phone: (403) 544-4913
Medicine Hat, AB fax: (403) 544-3388
Canada T1A 8K6 email: Fay.Schmaltz@dres.dnd.ca
===================================================================
=========================================================================
Date: Mon, 8 Feb 1999 13:57:51 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: Re: how to approach with tact?
MIME-Version: 1.0
Content-Type: text/plain
I received a request to look into possible bio contamination of a tissue
culture lab and an associated lab. Does this sound familiar?
It may be that there is poor ventilation. It may be that some microorganism
could have taken up residence in the CO2 incubators.
But, the fact of the matter, is that on the face of it, the place is a mess.
I almost wanted to put on blue jeans, a work shirt, rubber gloves, and start
putting "stuff" in the dumpster.
However, admonishing PIs doesn't work well (neither does admonishing teen
age children!)
So, while I have immense empathy for the constraints of time and space, how
do I best get the message across that they need to do some housekeeping?
=========================================================================
Date: Mon, 8 Feb 1999 16:13:11 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Robin Newberry
Subject: Re: how to approach with tact?
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
>But, the fact of the matter, is that on the face of it, the place is a mess.
>However, admonishing PIs doesn't work well.
I wouldn't "admonish" them, but I would point out that I couldn't begin to
make a valid assessment of the possible source of contamination until all
potential sources of contamination which were under the control of the PI
were eliminated. i.e. the lab was clean. Otherwise my report would probably
come back to them with language like "While the exact source of the
contamination could not be determined owing to many potential sources
inside the lab itself, it is the investigator's belief that poor
housekeeping and concomitant cross contamination of cultures is extremely
likely."
Make sure the Dean gets a copy. 8-)
Robin
W. Robert Newberry, IV CIH, CHMM
Director, Environmental Health and Safety
Clemson University
wnewber@clemson.edu ehs@clemson.edu
=========================================================================
Date: Tue, 9 Feb 1999 10:29:52 +1300
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stu Macdiarmid
Subject: Re: how to approach with tact? -Reply
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: quoted-printable
"... how do I best get the message across..."
Probably not by starting out with a request like this broadcast to the =
world. It is, after all, possible that these messy, almost teenage, lab =
workers may also subscribe to this public list. If so, any notion of =
"tact" is out the window!
Cheers
***************************************************************
Stuart C MacDiarmid
National Manager (Agricultural Security)
Regulatory Authority
Ministry of Agriculture and Forestry
PO Box 2526
Wellington
New Zealand.
Phone ;+64-4-474 4100
Fax ;+64-4-474 4133
Email ; macdiarmids@t.nz,
StuartMacDiarmid@
***************************************************************
>>> "Therese M. Stinnett" 9/February/1999 =
09:57am >>>
I received a request to look into possible bio contamination of a tissue
culture lab and an associated lab. Does this sound familiar?
It may be that there is poor ventilation. It may be that some microorganis=
m
could have taken up residence in the CO2 incubators.
But, the fact of the matter, is that on the face of it, the place is a =
mess.
I almost wanted to put on blue jeans, a work shirt, rubber gloves, and =
start
putting "stuff" in the dumpster.
However, admonishing PIs doesn't work well (neither does admonishing teen
age children!)
So, while I have immense empathy for the constraints of time and space, =
how
do I best get the message across that they need to do some housekeeping?
=========================================================================
Date: Mon, 8 Feb 1999 15:38:25 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: how to approach with tact?
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Dear Therese:
Rx No. 1: Good Housekeeping is a major component of Good Microbiological
Practices when biohazardous materials are in use [See BMBL].
Rx. No. 2: Good housekeeping practices are REQUIRED for compliance if
bloodborne pathogens, or human blood, body fluids or tissues are used in
the labs in question. Ask the good doctor running the lab to see his/her
OSHA required Housekeeping Plan. No plan, no compliance! No compliance
with plan, if it exists, is a violation of OSHA Law. Use the OSHA
inspector's checklist which includes many housekeeping details. If all
else [common sense] fails, have your Institutional Biosafety Committee do
their required duty to hold an inspection and shut the operation down if
needed to make the Lab environment "Hygienic". No hygiene -- No Usee till
it is hygienic! Blame the NIH, CDC and OSHA and tell the PI to take it
up with OSHA or the institution's management and the IBC. That
de-personalizes the issue with the safety Department.
Rx no. 3: Remind the PI scientist that good microbiological or
tissue culture research is, in part, no better than the cleanliness of
the facility where the work is done. As the biosafety Officer is charged
with compliance, bite the bullet and tell them to clean it up or make a
trip with you to the Chief of Safety to see who is right. Biosafety is
"driven" by the lab supervisor as a first order of biosafety management.
Good Luck!
Joe Coggin, Jr. Ph.D., RBP, CBSP and PI of a research lab complex.
On Mon, 8 Feb 1999, Therese M. Stinnett wrote:
> I received a request to look into possible bio contamination of a tissue
> culture lab and an associated lab. Does this sound familiar?
>
> It may be that there is poor ventilation. It may be that some microorganism
> could have taken up residence in the CO2 incubators.
>
> But, the fact of the matter, is that on the face of it, the place is a mess.
> I almost wanted to put on blue jeans, a work shirt, rubber gloves, and start
> putting "stuff" in the dumpster.
>
> However, admonishing PIs doesn't work well (neither does admonishing teen
> age children!)
>
> So, while I have immense empathy for the constraints of time and space, how
> do I best get the message across that they need to do some housekeeping?
>
=========================================================================
Date: Mon, 8 Feb 1999 16:55:13 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Francis Churchill
Subject: Re: how to approach with tact?
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
In my recent bout with this problem, I pointed out all of the places that
could serve as sinks for fungi, while looking for the source. I wrote up
ALL of my recommendations, from facilities improvement to remove the
cardboard boxes.
Hope it helps,
Francis
Alcohol and calculus don't mix. Never drink and derive.
Francis Churchill, IHIT
University of Vermont - Environmental Safety Facility
655D Spear Street, Burlington, VT 05405-3010
(802) 656-5405
fchurchi@zoo.uvm.edu
=========================================================================
Date: Mon, 8 Feb 1999 16:01:25 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: Re: how to approach with tact?
MIME-Version: 1.0
Content-Type: text/plain
Thanks for the quick response.
> -----Original Message-----
> From: Joseph H. Coggin Jr. [SMTP:jcoggin@JAGUAR1.USOUTHAL.EDU]
> Sent: Monday, February 08, 1999 2:38 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Re: how to approach with tact?
>
> Dear Therese:
> Rx No. 1: Good Housekeeping is a major component of Good Microbiological
> Practices when biohazardous materials are in use [See BMBL].
> Rx. No. 2: Good housekeeping practices are REQUIRED for compliance if
> bloodborne pathogens, or human blood, body fluids or tissues are used in
> the labs in question. Ask the good doctor running the lab to see his/her
>
>
oops--we're not an OSHA state, and as a state institution, I have no
OSHA to back me up
> OSHA required Housekeeping Plan. No plan, no compliance! No compliance
> with plan, if it exists, is a violation of OSHA Law. Use the OSHA
> inspector's checklist which includes many housekeeping details.
>
where would I find such a checklist?
> If all
> else [common sense] fails, have your Institutional Biosafety Committee do
> their required duty to hold an inspection and shut the operation down if
> needed to make the Lab environment "Hygienic". No hygiene -- No Usee till
> it is hygienic! Blame the NIH, CDC and OSHA and tell the PI to take it
> up with OSHA or the institution's management and the IBC. That
> de-personalizes the issue with the safety Department.
>
well--he's on the IBC....
> Rx no. 3: Remind the PI scientist that good microbiological or
> tissue culture research is, in part, no better than the cleanliness of
> the facility where the work is done. As the biosafety Officer is charged
> with compliance, bite the bullet and tell them to clean it up or make a
> trip with you to the Chief of Safety (I don't think my boss will back me
> up unless there is a law we have to abide by)
> to see who is right. Biosafety is
> "driven" by the lab supervisor as a first order of biosafety management.
>
>
> Good Luck!
>
> Joe Coggin, Jr. Ph.D., RBP, CBSP and PI of a research lab complex.
>
>
> On Mon, 8 Feb 1999, Therese M. Stinnett wrote:
>
> > I received a request to look into possible bio contamination of a tissue
> > culture lab and an associated lab. Does this sound familiar?
> >
> > It may be that there is poor ventilation. It may be that some
> microorganism
> > could have taken up residence in the CO2 incubators.
> >
> > But, the fact of the matter, is that on the face of it, the place is a
> mess.
> > I almost wanted to put on blue jeans, a work shirt, rubber gloves, and
> start
> > putting "stuff" in the dumpster.
> >
> > However, admonishing PIs doesn't work well (neither does admonishing
> teen
> > age children!)
> >
> > So, while I have immense empathy for the constraints of time and space,
> how
> > do I best get the message across that they need to do some housekeeping?
> >
=========================================================================
Date: Tue, 9 Feb 1999 18:02:39 +1100
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Le Blanc Smith, Peter"
Subject: Re: USE OF NITRILE GLOVES
MIME-Version: 1.0
Content-Type: text/plain
Probably the best information would come from a technical expert in the
company that manufactures the gloves. There may be specific gloves that
comply with biosafety regulations and standards whilst others may comply to
different standards e.g. chemical safety.
Some examples of statements in information sheets for N-DEX nitrile EMS
glove styles 9005 and 9005PF include "Meets all FDA Requirements for a Class
1 Medical Device; FDA 510K Document #K902696". "ideal for compliance with
OSHA Bloodborne Pathogen Standard 29CFR 1910.1030." "Biohazard resistant for
use in critical, high risk applications." "Passed the
bacteriophage-penetration resistance test. Note. Test conducted according to
ASTM ES 22-92 for NFPA 1999 Standard on Protective Clothing for Emergency
Medical Operations, 1992 Edition."
It would be prudent to collect good technical advice from the manufacturer
for each type of glove you intend to use.
Peter Le Blanc Smith
Biocontainment Microbiologist
CSIRO Animal Health
Australian Animal Health Laboratory
Private Mail Bag 24
Geelong Vic 3220
Australia
Ph: (03) 5227 5451 (including voice mail)
Int: +61 3 5227 5451
Fax: (03) 5227 5555
Int: +61 3 5227 5555
E-mail address. Peter.Le.Blanc.Smith@dah.csiro.au
-----Original Message-----
From: Ben Owens [SMTP:bowens@UNR.EDU]
Sent: Sunday, February 07, 1999 4:08 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: USE OF NITRILE GLOVES
Are thin (4 mil) nitrile gloves (such as the Best N-Dex or Ansell
Edmont
TNT) recognized as providing protection against bloodborne pathogens
and
other biological hazards (bacteria and viruses)? These nitrile
gloves
provide dexterity similar to latex exam gloves but offer increased
chemical resistance and also get away from potential latex allergy
problems. I have yet to see a real recommendation for use of the
nitrile gloves for protection against BBPs. NIOSH sent me many,
many
pages of information on latex allergies but no where did they
recommend
changing glove types (to nitrile or any other glove material). I'm
just
wondering if others are using the nitrile gloves for biological
hazards,
and if not, are there limitations to the nitrile gloves that I am
not
aware of? Thanks. Ben
--
Ben Owens, Chemical Hygiene Officer
University of Nevada, Reno
Environmental Health and Safety Department, MS 328
Reno, NV 89557
(702) 327-5196
(702) 784-4553 fax
=========================================================================
Date: Tue, 9 Feb 1999 09:00:07 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: how to approach with tact?
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Dear Therese again.
My State, Alabama, is also exempted from OSHA scrutiny for State owned or
operated facilities because the state signed an agreement with the
government called "Safe State". That does not mean that OSHA regulations
do not pertain!!!!! It means your state and mine agree to self-police and
use the OSHA required OR HIGHER safety standard. Hence, your state
facilities still must comply with the OSHA Law but OSHA will not enforce
the law because of your state's pledge to be fully responsible for doing
so. OSHA will only come to our campus if we have a work related death or
pattern of disease transmission but the State is responsible to the US
govt for its promise of compliance. Alabama stringently honors that
committment.
I just mailed you a copy of the OSHA Checklist. Call me if it isn't there
by week's end.
He's on the IB Committee. Great! Schedule him to audit your best and
cleanist lab along with several "informed" IBC members who understand
your problem. Get him to lead the audit and use the checklist. Then
have him tag along with the group when they do his annual physical
audit. He will likely see the "Light" and appreciate his negligence when
his fellow IBC members see the condition of his lab. Good Luck with your
difficult problem. Joe Coggin
On Mon, 8 Feb 1999, Therese M. Stinnett wrote:
> Thanks for the quick response.
>
> > -----Original Message-----
> > From: Joseph H. Coggin Jr. [SMTP:jcoggin@JAGUAR1.USOUTHAL.EDU]
> > Sent: Monday, February 08, 1999 2:38 PM
> > To: BIOSAFTY@MITVMA.MIT.EDU
> > Subject: Re: how to approach with tact?
> >
> > Dear Therese:
> > Rx No. 1: Good Housekeeping is a major component of Good Microbiological
> > Practices when biohazardous materials are in use [See BMBL].
> > Rx. No. 2: Good housekeeping practices are REQUIRED for compliance if
> > bloodborne pathogens, or human blood, body fluids or tissues are used in
> > the labs in question. Ask the good doctor running the lab to see his/her
> >
> >
> oops--we're not an OSHA state, and as a state institution, I have no
> OSHA to back me up
>
> > OSHA required Housekeeping Plan. No plan, no compliance! No compliance
> > with plan, if it exists, is a violation of OSHA Law. Use the OSHA
> > inspector's checklist which includes many housekeeping details.
> >
> where would I find such a checklist?
>
> > If all
> > else [common sense] fails, have your Institutional Biosafety Committee do
> > their required duty to hold an inspection and shut the operation down if
> > needed to make the Lab environment "Hygienic". No hygiene -- No Usee till
> > it is hygienic! Blame the NIH, CDC and OSHA and tell the PI to take it
> > up with OSHA or the institution's management and the IBC. That
> > de-personalizes the issue with the safety Department.
> >
> well--he's on the IBC....
>
> > Rx no. 3: Remind the PI scientist that good microbiological or
> > tissue culture research is, in part, no better than the cleanliness of
> > the facility where the work is done. As the biosafety Officer is charged
> > with compliance, bite the bullet and tell them to clean it up or make a
> > trip with you to the Chief of Safety (I don't think my boss will back me
> > up unless there is a law we have to abide by)
> > to see who is right. Biosafety is
> > "driven" by the lab supervisor as a first order of biosafety management.
> >
> >
> > Good Luck!
> >
> > Joe Coggin, Jr. Ph.D., RBP, CBSP and PI of a research lab complex.
> >
> >
> > On Mon, 8 Feb 1999, Therese M. Stinnett wrote:
> >
> > > I received a request to look into possible bio contamination of a tissue
> > > culture lab and an associated lab. Does this sound familiar?
> > >
> > > It may be that there is poor ventilation. It may be that some
> > microorganism
> > > could have taken up residence in the CO2 incubators.
> > >
> > > But, the fact of the matter, is that on the face of it, the place is a
> > mess.
> > > I almost wanted to put on blue jeans, a work shirt, rubber gloves, and
> > start
> > > putting "stuff" in the dumpster.
> > >
> > > However, admonishing PIs doesn't work well (neither does admonishing
> > teen
> > > age children!)
> > >
> > > So, while I have immense empathy for the constraints of time and space,
> > how
> > > do I best get the message across that they need to do some housekeeping?
> > >
>
=========================================================================
Date: Tue, 9 Feb 1999 10:32:19 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "P. Moravek"
Subject: Re: how to approach with tact?
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Based on Ms. Stinnett's description of the PI with the messy lab, I suggest that
she inundate the PI with citations from solid microbiology texts & articles that
address contamination from lab clutter and mess.
When those criteria are met, then one could test for contamination from other
sources.
Or, based on those cited criteria one could also justify clearing out the junk
yourself. Or, do a preliminary swab test from the surfaces of the actual junk
and see what grows. Present that information to the PI and let them make the
judgment.
Best of luck.
--P.Moravek Biological Safety Officer
Worcester Polytechnic Institute
=========================================================================
Date: Tue, 9 Feb 1999 12:44:34 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Fran Martin
Subject: 1998 U.S. EPA Regs on CD-ROM
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
FYI,
All 24 Volumes of Title 40 (Environmental Regulations/EPA) of the U.S. Code
of Federal Regulations have been updated to the latest 1998 revision levels.
Title 40 CFR is available as part of an "All 50 U.S. CFRs on CD-ROM"
offering. The CD-ROM has 5,000+ inline graphics, instant search/retrieval
with Adobe(R) Acrobat(R) Reader, and PDF format.
See for details.
Fran Martin
FM Research & Consulting
=========================================================================
Date: Tue, 9 Feb 1999 11:29:43 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Michael R. Betlach"
Subject: Inter/Intranet and/or MM Safety Training Survey
MIME-Version: 1.0
Content-Type: text/plain
Our EH&S Manager asked that I forward the survey below to the BioSafty
group. From presentations at the last two ABSA meetings and comments on the
mail list, many of you have direct experience with electronic training. At
present, we use our Intranet to disseminate safety information, but haven't
expanded the program to provide on demand training at the desk. Please reply
directly to Brian Olson. Thank you. I'll post results next month once he has
tabulated them.
Michael R. Betlach, Ph.D.
Biosafety Officer
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
(608) 274-1181, Ext. 1270
(608) 277-2677 FAX
mbetlach@
THIS SURVEY IS BEING SENT TO SEVERAL SAFETY MAIL-LISTS.
TO ENSURE YOUR SURVEY IS INCLUDED, PLEASE SEND IT TO bolson@
.
Good day folks. As a reader and (mild) participant of the safety mail-lists
for the past 5 years, I have a big favor to ask. My company is looking at
training options. This survey requires mostly one-word answers.
The survey looks at ELECTRONIC SAFETY TRAINING OPTIONS. I know this topic
has been talked about in some of the better known publications, but I am
interested in the feedback from you all.
I am sending this to four safety mail-lists. To assure your entry is
tabulated, please send your reply to bolson@
. I will be tabulating all responses and will
send them to the RADSAFE, BIOSAFETY, SAFETY, and LABSAFETY mail-lists by the
end of the month.
If you are using some type of electronic safety training, please take a
minute and complete the survey. Between the four mail-lists that are
receiving this request, I feel many could benefit from the results:
1. What type of institution are you with? (business, academia, large, small,
other)
2. What factor(s) did you consider before/while you moved to electronic
training?
(staffing?, location of buildings?, cost?, etc.)
3. Does your institution have access to Intranet? Internet? LAN? CD-ROM
Carousel?
4. What type of elec. training medium are you using? (Intranet, Internet,
LAN, CD-ROM, e-mail),
5. List some of the titles you offer: (e.g. lab safety, biosafety,
radsafety, hazcomm, dot, etc.),
6. Do you offer classroom and/or hands-on training in addition to the
electronic training, or does this replace it?
7. Did you purchase pre-made titles or did you create your own?
8. If pre-made, what software company(s) did you go with? (Marcom, Mastery,
Summit, J.J. Keller, other?)
9. If created by you, what authoring tools did you use?
(Director/Authorware, Dreamweaver, Frontpage, etc.)
10. Either way, on average, what was your cost per title? Total budget
(range)?
11. On average, how big is your audience - per title? 0-50, 51-99, 100-299,
>300
12. Do you use electronic training for annual refresher training?
13. Is changing content annually (for freshness) important to your audience?
Are you able to change your subject content when you want? Change test
questions? Both? Neither?
14. Overall, are you happy with your choice to go with electronic training?
(i.e. are you getting the results you want? high retention, etc.)
Thanks for your participation. I will tabulate and post all results (sent
to bolson@ ) by the end of February.
Thanks folks,
Brian Olson
Manager, EH&S
Promega Corp. (is a biotech co.)
Madison, WI
bolson@
=========================================================================
Date: Tue, 9 Feb 1999 16:55:07 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Brenda Barry
Subject: Re: 1998 U.S. EPA Regs on CD-ROM
MIME-Version: 1.0
Content-Type: text/plain
Cynthia,
FYI.
Brenda
> ----------
> From: Fran Martin[SMTP:fmartin@]
> Sent: Tuesday, February 09, 1999 12:44 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: 1998 U.S. EPA Regs on CD-ROM
>
> FYI,
>
> All 24 Volumes of Title 40 (Environmental Regulations/EPA) of the U.S.
> Code
> of Federal Regulations have been updated to the latest 1998 revision
> levels.
> Title 40 CFR is available as part of an "All 50 U.S. CFRs on CD-ROM"
> offering. The CD-ROM has 5,000+ inline graphics, instant
> search/retrieval
> with Adobe(R) Acrobat(R) Reader, and PDF format.
>
> See for details.
>
> Fran Martin
> FM Research & Consulting
>
=========================================================================
Date: Wed, 10 Feb 1999 08:47:33 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Mike Miller
Subject: Re: USE OF NITRILE GLOVES
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
Yes, but two 4 mil gloves gives an 8 mil rating.
Mike Miller
=========================================================================
Date: Thu, 11 Feb 1999 11:00:57 -0000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stuart Thompson
Subject: Re: Inter/Intranet and/or MM Safety Training Survey
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Yesterday, I went to a meeting of the British Health & Safety Society at
which Bob Forster of the BBC (British Broadcasting Corporation) gave a very
impressive talk and demonstration of a CD-ROM course developed for the
broadcasting industry. Although designed as a modular self-training course
for managers in radio and TV, much of it gives a very good introduction to
general concepts of risk assessment and safety management. Any institution
that has researchers who travel to study biomedical problems in third world
countries could learn a lot from this. Health hazards and the dangers of
unfamiliar geographical environments, terrorists and armed revolutionaries
can be as big a problem for researchers as for news reporters and camera
operators. In both cases, careful pre-planning can reduce risks to
acceptable levels.
Further details are at:
bbc.co.uk/training
Best wishes
Stuart
Dr Stuart Thompson
Health & Safety Services
University of Manchester
Waterloo Place
182/184 Oxford Road
Manchester M13 9PG
tel: +44 (0)161 275 5069
fax: +44 (0)161 275 6989
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Michael R. Betlach
Sent: 09 February 1999 17:30
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Inter/Intranet and/or MM Safety Training Survey
Our EH&S Manager asked that I forward the survey below to the BioSafty
group. From presentations at the last two ABSA meetings and comments on the
mail list, many of you have direct experience with electronic training. At
present, we use our Intranet to disseminate safety information, but haven't
expanded the program to provide on demand training at the desk. Please reply
directly to Brian Olson. Thank you. I'll post results next month once he has
tabulated them.
Michael R. Betlach, Ph.D.
Biosafety Officer
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
(608) 274-1181, Ext. 1270
(608) 277-2677 FAX
mbetlach@
THIS SURVEY IS BEING SENT TO SEVERAL SAFETY MAIL-LISTS.
TO ENSURE YOUR SURVEY IS INCLUDED, PLEASE SEND IT TO bolson@
.
Good day folks. As a reader and (mild) participant of the safety mail-lists
for the past 5 years, I have a big favor to ask. My company is looking at
training options. This survey requires mostly one-word answers.
The survey looks at ELECTRONIC SAFETY TRAINING OPTIONS.
=========================================================================
Date: Thu, 11 Feb 1999 07:53:00 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Mann
Subject: Copy of: [COMPMED] Respiratory Protective Masks
I have been asked to forward this question by a collegue of mine who is
presently not on the list.
Please direct responses to her.
Thanks
Richard Mann,DVM
VMO
VA Med Ctr Northport NY
Hello again,
When it rains it pours. This is my week to battle the establishment.
Here is the issue.
I require all personnel to wear NIOSH certified N95 Particulate
Respirators when in all our animal rooms. These masks must be
fit-tested. Our IH office has decided that only personnel with known
allergies to laboratory animals need where a respirator. I explained
that use of these masks is as much a preventive measure for nonaffected
workers as it is a protection measure for affected workers. I sighted
the NRC Occupational Health and Safety in the Care and Use of Research
Animals. I pounded the table with my shoe. Still they will not listen.
So it is with that synopsis that I come to "The List". My question is:
Do you require your animal caretaker/technician/veterinarian work force
to use respiratory protective equipment in conventional animal rooms?
If not, what is your institutional policy with respect to PPE in animal
rooms?
If yes, what type of mask/respirator do you use and is it fit tested?
If your workers wear fit-tested NIOSH approved respirators do you
require them to be clean-shaven?
Ten years ago, a mentor of mine, Dr. Mike Flynn, insisted that surgical
masks were no good and everyone at the facility where we worked must
wear respiratory protective masks. I took that lesson home to the bank
and have always applied it. Please educate me if I have been wrong all
these years or give me more ammunition to convince our IH of the need
for this program.
I will summarize the results and send to the list.
Thanks in advance for your help.
Carol Eisenhauer, DVM, DACLAM
Chief, Div of Lab Animal Medicine
Armed Forces Institute of Pathology
Washington, DC 20306
202-782-2235
eisenhauerc@afip.osd.mil
=========================================================================
Date: Thu, 11 Feb 1999 08:08:20 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Re: Copy of: [COMPMED] Respiratory Protective Masks
We recommend the use of respirators when working with animals - especially
when handling the animal (whether in transport, surgery or testing).
We actually did a presentation for the site this past month regarding animal
allergies. This all came about from the NIOSH Alert in January 1998
(attached below). I have also attached benchmarking from other US
pharmaceutical companies. Let me know if I can be of any further
assistance!
Bliss M. Schlank
Biosafety Specialist
Zeneca Pharmaceuticals
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185
Fax: 302.886.2909
bliss.schlank@phwilm.
> ----------
> From: Richard Mann[SMTP:MANN.RICHARD@NORTHPORT.]
> Sent: Thursday, February 11, 1999 7:53 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Copy of: [COMPMED] Respiratory Protective Masks
>
> I have been asked to forward this question by a collegue of mine who is
> presently not on the list.
>
> Please direct responses to her.
>
> Thanks
>
> Richard Mann,DVM
> VMO
> VA Med Ctr Northport NY
>
>
>
>
> Hello again,
>
> When it rains it pours. This is my week to battle the establishment.
> Here is the issue.
>
> I require all personnel to wear NIOSH certified N95 Particulate
> Respirators when in all our animal rooms. These masks must be
> fit-tested. Our IH office has decided that only personnel with known
> allergies to laboratory animals need where a respirator. I explained
> that use of these masks is as much a preventive measure for nonaffected
> workers as it is a protection measure for affected workers. I sighted
> the NRC Occupational Health and Safety in the Care and Use of Research
> Animals. I pounded the table with my shoe. Still they will not listen.
>
> So it is with that synopsis that I come to "The List". My question is:
>
> Do you require your animal caretaker/technician/veterinarian work force
> to use respiratory protective equipment in conventional animal rooms?
>
> If not, what is your institutional policy with respect to PPE in animal
> rooms?
>
> If yes, what type of mask/respirator do you use and is it fit tested?
>
> If your workers wear fit-tested NIOSH approved respirators do you
> require them to be clean-shaven?
>
> Ten years ago, a mentor of mine, Dr. Mike Flynn, insisted that surgical
> masks were no good and everyone at the facility where we worked must
> wear respiratory protective masks. I took that lesson home to the bank
> and have always applied it. Please educate me if I have been wrong all
> these years or give me more ammunition to convince our IH of the need
> for this program.
>
> I will summarize the results and send to the list.
>
> Thanks in advance for your help.
>
> Carol Eisenhauer, DVM, DACLAM
> Chief, Div of Lab Animal Medicine
> Armed Forces Institute of Pathology
> Washington, DC 20306
> 202-782-2235
> eisenhauerc@afip.osd.mil
>
Attachment Converted: "E:\Eudora\Attach\Animal Allergy Comparison US PHARM.doc"
=========================================================================
Date: Thu, 11 Feb 1999 09:17:03 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Daniel King
Subject: Re: Copy of: [COMPMED] Respiratory Protective Masks
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Translation of attachment USPHARM.DOC garbled (unreadable) with my email
system.
Daniel J. (Jack) King
USDA, ARS, Southeast Poultry Research Laboratory
934 College Station Road
Athens, GA 30605
706-546-3407 Phone
706-546-3161 FAX
dking@asrr.
On Thu, 11 Feb 1999, Schlank Bliss BM wrote:
> We recommend the use of respirators when working with animals - especially
> when handling the animal (whether in transport, surgery or testing).
>
> We actually did a presentation for the site this past month regarding animal
> allergies. This all came about from the NIOSH Alert in January 1998
> (attached below). I have also attached benchmarking from other US
> pharmaceutical companies. Let me know if I can be of any further
> assistance!
>
>
>
> Bliss M. Schlank
> Biosafety Specialist
> Zeneca Pharmaceuticals
> 1800 Concord Pike
> Wilmington DE 19850-5437
> 302.886.2185
> Fax: 302.886.2909
> bliss.schlank@phwilm.
>
> > ----------
> > From: Richard Mann[SMTP:MANN.RICHARD@NORTHPORT.]
> > Sent: Thursday, February 11, 1999 7:53 AM
> > To: BIOSAFTY@MITVMA.MIT.EDU
> > Subject: Copy of: [COMPMED] Respiratory Protective Masks
> >
> > I have been asked to forward this question by a collegue of mine who is
> > presently not on the list.
> >
> > Please direct responses to her.
> >
> > Thanks
> >
> > Richard Mann,DVM
> > VMO
> > VA Med Ctr Northport NY
> >
> >
> >
> >
> > Hello again,
> >
> > When it rains it pours. This is my week to battle the establishment.
> > Here is the issue.
> >
> > I require all personnel to wear NIOSH certified N95 Particulate
> > Respirators when in all our animal rooms. These masks must be
> > fit-tested. Our IH office has decided that only personnel with known
> > allergies to laboratory animals need where a respirator. I explained
> > that use of these masks is as much a preventive measure for nonaffected
> > workers as it is a protection measure for affected workers. I sighted
> > the NRC Occupational Health and Safety in the Care and Use of Research
> > Animals. I pounded the table with my shoe. Still they will not listen.
> >
> > So it is with that synopsis that I come to "The List". My question is:
> >
> > Do you require your animal caretaker/technician/veterinarian work force
> > to use respiratory protective equipment in conventional animal rooms?
> >
> > If not, what is your institutional policy with respect to PPE in animal
> > rooms?
> >
> > If yes, what type of mask/respirator do you use and is it fit tested?
> >
> > If your workers wear fit-tested NIOSH approved respirators do you
> > require them to be clean-shaven?
> >
> > Ten years ago, a mentor of mine, Dr. Mike Flynn, insisted that surgical
> > masks were no good and everyone at the facility where we worked must
> > wear respiratory protective masks. I took that lesson home to the bank
> > and have always applied it. Please educate me if I have been wrong all
> > these years or give me more ammunition to convince our IH of the need
> > for this program.
> >
> > I will summarize the results and send to the list.
> >
> > Thanks in advance for your help.
> >
> > Carol Eisenhauer, DVM, DACLAM
> > Chief, Div of Lab Animal Medicine
> > Armed Forces Institute of Pathology
> > Washington, DC 20306
> > 202-782-2235
> > eisenhauerc@afip.osd.mil
> >
>
> begin 600 Animal Allergy Comparison US PHARM.doc
> MT,\1X*&Q&N$`````````````````````/@`#`/[_"0`&```````````````!
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> M`$-/35!!3ED)``,`````````````````````````````````````````````
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> `
> end
>
=========================================================================
Date: Thu, 11 Feb 1999 09:26:46 -0600
Reply-To: "mkinsey@"
Sender: A Biosafety Discussion List
From: Melina Kinsey
Organization: MRI
Subject: Agarose gel electrophoresis
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Biosafety group-
One of our microbiologist is involved with a community project which promotes
science and technology to high school students by putting on informative
workshops. She would like to demonstrate how to run agarose gel
electrophoresis using molecular weight markers obtained commercially. She
would like to perform this in one of our conference rooms on a table with three
groups of 10 kids (13-17 years). The Institutes does not allow children under
the age of 18 in our laboratories, so this was not an option when the workshop
was set up. The microbiologist would like to give the kids some hands on
experience by allowing them to load one lane of the gel. The microbiologist
and the kids would wear a lab coats, gloves and eye protection during this
entire operation.
I have done some reading and ethidium bromide seems to me the most hazardous
material used during this process. However, the protective clothing (coats,
gloves, safety glasses) seemed appropriate.
This is where I need help. I am not that familiar with gel electrophoresis and
the hazards. At this time I have not approved this demonstration for the
workshop and won't until I know a little more about the hazards.
If you can help straighten me out, please email the list or me privately at
mkinsey@.
Thanks in advance for your help.
Melina Kinsey
Sr. Biosafey Technician
Midwest Research Institute
mkinsey@
=========================================================================
Date: Thu, 11 Feb 1999 07:56:19 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Melinda Young
Subject: By-products of autoclaving plastics
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Greetings:
I am looking for any references on this subject. Please respond directly
to me and I will summarize for the group as all might not be interested.
We have been working with a group of maintenance employees who have lots
of concerns about their health. One question was that off-gassing or
break down of plastics during the autoclaving process. This is an
autoclave that processes waste from a hospital but does not do laboratory
waste. There will be a lot of syringes, sharps containers and then
various disposable plastics from surgery, etc.
We did some looking and came up with that off gassing should not be a
concern if the temperature is below 300F and the autoclave operates below
that temperature.
Melinda Young
______________________________________________________________________________
Melinda Young, CBSP University of Washington
Biosafety Supervisor Environmental Health and Safety
Prevention and Assessment Office Box 354400
biosafe@u.washington.edu Seattle, WA 98195-4400
Voice Mail (206)543-7278
Fax (206)616-3360
Phone (206)543-9510
Office: Rm 411 of Hall Health Center my schedule 7:45-4:45
=========================================================================
Date: Thu, 11 Feb 1999 08:54:55 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: By-products of autoclaving plastics
MIME-Version: 1.0
Content-Type: text/plain
Hi, Melinda -
At the 1996 ABSA meeting in Salt Lake City, Julia Hadar from the Dept.
of Safety and Occupational Health, Hebrew University of Jerusalem,
Israel, presented a paper entitled "Emissions from Autoclaves -
Hazardous or Not?" She noted that high and low-density polyethylene,
acrylic, polystyrene, polyvinyl chloride and other plastics are unstable
above 120 degrees C and should not be autoclaved. If my notes are
accurate, she also said, based on some information she obtained from
NUNC, that an autoclave load of only 20 styrene petri plates could
release up to 6000 mg/cubic meter of styrene, well above the TLV. She
recommended the use of local active ventilation and vacating the
autoclave area after opening the unit for at least 10 minutes. I
haven't heard or read anything from her since then so I don't know if
she's continuing this line of research. I'd be happy to fax you the
brief abstract from the conference proceedings if you don't have them -
it's on page 48.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Melinda Young [mailto:biosafe@U.WASHINGTON.EDU]
Sent: Thursday, February 11, 1999 7:56 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: By-products of autoclaving plastics
Greetings:
I am looking for any references on this subject. Please respond
directly
to me and I will summarize for the group as all might not be interested.
We have been working with a group of maintenance employees who have lots
of concerns about their health. One question was that off-gassing or
break down of plastics during the autoclaving process. This is an
autoclave that processes waste from a hospital but does not do
laboratory
waste. There will be a lot of syringes, sharps containers and then
various disposable plastics from surgery, etc.
We did some looking and came up with that off gassing should not be a
concern if the temperature is below 300F and the autoclave operates
below
that temperature.
Melinda Young
________________________________________________________________________
______
Melinda Young, CBSP University of Washington
Biosafety Supervisor Environmental Health and Safety
Prevention and Assessment Office Box 354400
biosafe@u.washington.edu Seattle, WA 98195-4400
Voice Mail (206)543-7278
Fax (206)616-3360
Phone (206)543-9510
Office: Rm 411 of Hall Health Center my schedule 7:45-4:45
=========================================================================
Date: Thu, 11 Feb 1999 12:51:58 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Leslie Delpin
Subject: Caro Safe
Good day all.
Does anyone have information (in particular, disposal info)
concerning a product called "Caro Safe"? It is used as a specimen
transportmedium/perservative?. The MSDS reference I found
(Vermont SIRI) is inaccurate.
Leslie Delpin - Biological Health and Safety Manager
University of Connecticut
Environmental Health and Safety
3102 Horsebarn Hill Rd. Box U-97
Storrs, CT 06269-4097
(860)486-3613
(860)486-1106
lmdelpin@safety.ehs.uconn.edu
=========================================================================
Date: Thu, 11 Feb 1999 13:15:15 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Alan Woodard
Subject: Re: By-products of autoclaving plastics -Reply
Mime-Version: 1.0
Content-Type: text/plain
I would appreciate a copy of the abstract too. Also, do you have an
e-mail address for Ms. Hadar?
Alan G. Woodard, Ph.D.
Regulated Medical Waste Program Supervisor
New York State
Department of Environmental Conservation
Division of Solid & Hazardous Materials
50 Wolf Road, Room 206
Albany, New York 12233-7258
Phone: (518) 457-5695
Fax: (518) 485-7723
E-mail: agwoodar@gw.dec.state.ny.us
>>> "Funk, Glenn" 02/11/99 11:54am
>>>
Hi, Melinda -
At the 1996 ABSA meeting in Salt Lake City, Julia Hadar from the Dept.
of Safety and Occupational Health, Hebrew University of Jerusalem,
Israel, presented a paper entitled "Emissions from Autoclaves -
Hazardous or Not?" She noted that high and low-density polyethylene,
acrylic, polystyrene, polyvinyl chloride and other plastics are unstable
above 120 degrees C and should not be autoclaved. If my notes are
accurate, she also said, based on some information she obtained from
NUNC, that an autoclave load of only 20 styrene petri plates could
release up to 6000 mg/cubic meter of styrene, well above the TLV. She
recommended the use of local active ventilation and vacating the
autoclave area after opening the unit for at least 10 minutes. I
haven't heard or read anything from her since then so I don't know if
she's continuing this line of research. I'd be happy to fax you the
brief abstract from the conference proceedings if you don't have them -
it's on page 48.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Melinda Young [mailto:biosafe@U.WASHINGTON.EDU]
Sent: Thursday, February 11, 1999 7:56 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: By-products of autoclaving plastics
Greetings:
I am looking for any references on this subject. Please respond
directly
to me and I will summarize for the group as all might not be interested.
We have been working with a group of maintenance employees who
have lots
of concerns about their health. One question was that off-gassing or
break down of plastics during the autoclaving process. This is an
autoclave that processes waste from a hospital but does not do
laboratory
waste. There will be a lot of syringes, sharps containers and then
various disposable plastics from surgery, etc.
We did some looking and came up with that off gassing should not be a
concern if the temperature is below 300F and the autoclave operates
below
that temperature.
Melinda Young
________________________________________________________________________
______
Melinda Young, CBSP University of Washington
Biosafety Supervisor Environmental Health and Safety
Prevention and Assessment Office Box 354400
biosafe@u.washington.edu Seattle, WA 98195-4400
Voice Mail (206)543-7278
Fax (206)616-3360
Phone (206)543-9510
Office: Rm 411 of Hall Health Center my schedule 7:45-4:45
=========================================================================
Date: Thu, 11 Feb 1999 13:46:27 +0000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stan Harts
Organization: University of North Carolina at Wilmington
Subject: Re: Caro Safe
In-Reply-To:
MIME-version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
Carosafe is a an alcohol based biological specimen storage liquid.
My understanding is that the specimens are fixed in a fomaldehyde
solution, rinsed in water and then placed in Carosafe for
preservation. The main component are propylene glycol and water.
Carolina biological has 2 MSDSs for Carosafe. One for carosafe only
and one for "Specimens Preserved in Carosafe". The later includes
references to small amounts of formaldehyde carried over from the
fixative process.
Carolina Biological's phone number is 1-800-334-5551 or I have the
MSDSs.
We separate the specimen solids from the waste and mix the liquid
with a haz-waste waste stream containing formaldehyde, water,
glychols, phenol, methanol and other biological specimen fluids. The
cost of disposal is pretty economical at about $130 per drum.
Stan H. Harts
Environmental Safety Manager
University of North Carolina at Wilmington
601 South College Road
Wilmington, NC 28403-3297
E-Mail: Hartss@uncwil.edu
Phone: (910) 962-7017
Fax: (910) 962-4014
=========================================================================
Date: Thu, 11 Feb 1999 10:51:47 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Sandra Fry
Subject: Re: By-products of autoclaving plastics
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; CHARSET=ISO-8859-1
The paper that Julia presented on "Autoclave Emissions, Hazardous or Not?"
has been published in the ABSA Journal, Volume 2, Number 3, 1997 pages 44-51
------------------------
From: "Funk, Glenn"
Subject: Re: By-products of autoclaving plastics
Date: Thu, 11 Feb 1999 08:54:55 -0800
To: BIOSAFTY@mitvma.mit.edu
> Hi, Melinda -
>
> At the 1996 ABSA meeting in Salt Lake City, Julia Hadar from the Dept.
> of Safety and Occupational Health, Hebrew University of Jerusalem,
> Israel, presented a paper entitled "Emissions from Autoclaves -
> Hazardous or Not?" She noted that high and low-density polyethylene,
> acrylic, polystyrene, polyvinyl chloride and other plastics are unstable
> above 120 degrees C and should not be autoclaved. If my notes are
> accurate, she also said, based on some information she obtained from
> NUNC, that an autoclave load of only 20 styrene petri plates could
> release up to 6000 mg/cubic meter of styrene, well above the TLV. She
> recommended the use of local active ventilation and vacating the
> autoclave area after opening the unit for at least 10 minutes. I
> haven't heard or read anything from her since then so I don't know if
> she's continuing this line of research. I'd be happy to fax you the
> brief abstract from the conference proceedings if you don't have them -
> it's on page 48.
>
> -- Glenn
>
> -----------------------------------------------
> Glenn A. Funk, Ph.D., CBSP
> Biosafety Officer
> U.C. San Francisco
> 415-476-2097 fax 415-476-0581
> glennf@ehsmail.ucsf.edu
>
>
> -----Original Message-----
> From: Melinda Young [mailto:biosafe@U.WASHINGTON.EDU]
> Sent: Thursday, February 11, 1999 7:56 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: By-products of autoclaving plastics
>
>
> Greetings:
>
> I am looking for any references on this subject. Please respond
> directly
> to me and I will summarize for the group as all might not be interested.
>
> We have been working with a group of maintenance employees who have lots
> of concerns about their health. One question was that off-gassing or
> break down of plastics during the autoclaving process. This is an
> autoclave that processes waste from a hospital but does not do
> laboratory
> waste. There will be a lot of syringes, sharps containers and then
> various disposable plastics from surgery, etc.
>
> We did some looking and came up with that off gassing should not be a
> concern if the temperature is below 300F and the autoclave operates
> below
> that temperature.
>
> Melinda Young
>
>
> ________________________________________________________________________
> ______
>
> Melinda Young, CBSP University of Washington
> Biosafety Supervisor Environmental Health and Safety
> Prevention and Assessment Office Box 354400
> biosafe@u.washington.edu Seattle, WA 98195-4400
>
> Voice Mail (206)543-7278
> Fax (206)616-3360
> Phone (206)543-9510
>
> Office: Rm 411 of Hall Health Center my schedule 7:45-4:45
---------------End of Original Message-----------------
Sandra C. Fry
Biosafety Officer
BC Centre for Disease Control,
655 W. 12th Avenue
Vancouver, BC V5Z 4R4
phone: (604) 660 6167
pager: (604) 893 5737
fax: (604) 660 6073
=========================================================================
Date: Thu, 11 Feb 1999 14:12:32 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Sandy Dempsey
Subject: Re: Caro Safe
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Hi, Leslie. I believe Caro-safe is the preservative used by Carolina
Biological Supply Co.
Their number is 1-800-334-5551. Cheers, Sandy
Leslie Delpin wrote:
> Good day all.
>
> Does anyone have information (in particular, disposal info)
> concerning a product called "Caro Safe"? It is used as a specimen
> transportmedium/perservative?. The MSDS reference I found
> (Vermont SIRI) is inaccurate.
> Leslie Delpin - Biological Health and Safety Manager
> University of Connecticut
> Environmental Health and Safety
> 3102 Horsebarn Hill Rd. Box U-97
> Storrs, CT 06269-4097
>
> (860)486-3613
> (860)486-1106
> lmdelpin@safety.ehs.uconn.edu
=========================================================================
Date: Thu, 11 Feb 1999 14:07:42 -0600
Reply-To: "mkinsey@"
Sender: A Biosafety Discussion List
From: Melina Kinsey
Organization: MRI
Subject: Thanks!!
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Biosafety Group-
Thanks so much for your responses on my question about agarose gel
electrophoresis. I had so many that responded that I couldn't get to all of
you individually to thank you. Since I signed up a year ago, this list has
been my daily educational source. The responses I received on my question was
able to help me discuss an appropriate outline with the microbiologist for her
demonstration.
First she will discuss the steps the she followed to set-up the experiment,
such as reviewing chemical MSDS for hazards, set-up of equipment and proper
protective clothing. She will have the kids fill wells in a demo gel. She
will then run the gel and discuss the electrical hazard associated with this
process. They will get the experience but will not be exposed to any hazardous
chemicals in the process. The microbiologist will show the kids a picture of
what the results look like after the whole process is complete. This will keep
the kids away from the UV gel reader and protect their eyes.
All of your suggestions helped us plan a safe demonstration. To all who
responded, thanks for the great education and help you provided.
Melina Kinsey
Sr. Biosafey Technician
Midwest Research Institute
mkinsey@
=========================================================================
Date: Thu, 11 Feb 1999 16:31:59 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Leslie Delpin
Subject: Re: Caro Safe
In-Reply-To:
Thanks Sandy.
The MSDS that comes up for this material lists
Carolina Chemical Supply Co as the supplier and the cross reference
product name "fast dry safety solvent". When I called the
company, they had no knowledge of "caro safe"
Leslie Delpin - Biological Health and Safety Manager
University of Connecticut
Environmental Health and Safety
3102 Horsebarn Hill Rd. Box U-97
Storrs, CT 06269-4097
(860)486-3613
(860)486-1106
lmdelpin@safety.ehs.uconn.edu
=========================================================================
Date: Thu, 11 Feb 1999 16:38:32 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Leslie Delpin
Subject: Re: Caro Safe
In-Reply-To:
Many thanks Stan.
Leslie Delpin - Biological Health and Safety Manager
University of Connecticut
Environmental Health and Safety
3102 Horsebarn Hill Rd. Box U-97
Storrs, CT 06269-4097
(860)486-3613
(860)486-1106
lmdelpin@safety.ehs.uconn.edu
=========================================================================
Date: Fri, 12 Feb 1999 09:21:49 EDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jennifer Reader
Organization: Environmental Health and Safety
Subject: zoonotics
MIME-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
Dear group,
I would like to know what other institutions are doing about
zoonotics.
Currently, we register projects where people are cooking up
things as cultures. We do not register projects involving
potentially biohazardous material such as dead squirrels.
Do you register projects for such things as dead squirrels, live
turtles, healthy chickens (which may have salmonella), etc?
Keep in mind we have a vet teaching hospital so anything can
show up at the door there too.
If you register the project, is it in a different format than
conventional, lab-based, culture work?
Thanks. As you can see, this can be "loaded" politically.
Jennifer Reader, B.S., M.S.P.H.
Hazardous Materials Safety Officer
Environmental Health and Safety
University of Guelph
Guelph, Ontario N1G 2W1 Canada
519-824-4120 X3190 Fax 519-824-0364
e-Mail jennifer@ehs.uoguelph.ca
=========================================================================
Date: Fri, 12 Feb 1999 10:04:40 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph P. Kozlovac"
Subject: Re: zoonotics
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Does your institution have an Animal Care and Use Committee...this is the
group which provides oversight on animal work conducted at our facility.
We have also developed formalized administrative mechanisms in which our
biosafety committee and the ACUC communicate and exchange information on
various projects to ensure appropriate registration.
At 09:21 12.2.1999 -0400, you wrote:
>Dear group,
>
>I would like to know what other institutions are doing about
>zoonotics.
>
>Currently, we register projects where people are cooking up
>things as cultures. We do not register projects involving
>potentially biohazardous material such as dead squirrels.
>
>Do you register projects for such things as dead squirrels, live
>turtles, healthy chickens (which may have salmonella), etc?
>Keep in mind we have a vet teaching hospital so anything can
>show up at the door there too.
>
>If you register the project, is it in a different format than
>conventional, lab-based, culture work?
>
>Thanks. As you can see, this can be "loaded" politically.
>
>
>
>
>Jennifer Reader, B.S., M.S.P.H.
>Hazardous Materials Safety Officer
>Environmental Health and Safety
>University of Guelph
>Guelph, Ontario N1G 2W1 Canada
>519-824-4120 X3190 Fax 519-824-0364
>e-Mail jennifer@ehs.uoguelph.ca
______________________________________________________________________________
Biological Safety Officer
Safety and Environmental Protection Program
NCI - Frederick Cancer Research
and Development Center
(301)846-1451 fax: (301)846-6619
email: jkozlovac@mail.
______________________________________________________________________________
=========================================================================
Date: Fri, 12 Feb 1999 09:58:33 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: zoonotics
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Golden Rule No.6:
If in doubt about the biohazardous "nature" or risk potential of a material,
animal, tissue, or lab activity, etc get your research supervisors to be
comfortable and not alarmed to write your IBC or Safety Office a short
description telling you why they are concerned about the matter as a
potential biohazard and seeking an official opinion on things that
fall in the gray area [like zoonotic problems] rather than
filing a formal Biohazardous Agent Registration or BAR as we call them
for approval.
It is usually a good way to handle these matters.
Joe Coggin, Jr Ph.D.
=========================================================================
Date: Fri, 12 Feb 1999 11:20:47 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Andrew Cockburn
Subject: Re: zoonotics
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Our ACUC recently asked me and the IBC to look at projects involving wild
animals. They were especially concerned about "road kill" projects that seem
to fall outside of the ACUC mandate.
We decided that:
1) The Biosafety Officer (me) should be an ex officio member of the ACUC.
This allows me to check up on the projects that need biosafety approval.
2) All wild animal projects (living or dead) that involve possible zoonotic
contacts must be approved by the IBC. Here in West Virginia we are primarily
concerned with rabies and Hanta, so we will be looking carefully at use of
mammalian carnivores and rodents. I wrote a new protocol form to cover these
projects, which I will be glad to send to you. I based it on our human
tissue/sera protocol rather than our pathogen protocol.
3) The ACUC has authority over the importation of dead animals, cell lines,
or tissues onto campus if it may impact animal welfare. As an extreme
example, bringing a cow that died of anthrax onto the WVU farm would
endanger the WVU herd and would need to be approved by the ACUC (as well as
the IBC).
Andrew Cockburn, PhD
Director of Biological Safety
West Virginia University
Morgantown, WV 26506-9006
Telephone 304-293-7157
Email acockbur@wvu.edu
=========================================================================
Date: Fri, 12 Feb 1999 10:37:26 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Terry Howard
Subject: Health Sciences Center Design
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
We have the rare opportunity here to redesign our Health Sciences Center
from the ground up. Do any of you out there have suggestions about which
new facilities, hopefully multidisciplinary Health Science Centers, we
should look at as benchmarks of farsighted, efficient, and effective design?
We would like a few good examples of state of the art facilities. Thanks
for any help you can provide.
Terry L. Howard, CIH
Director, Health and Safety Division
UCHSC Department of Facilities Operations
4200 E. 9th Ave., C275
Denver, CO 80262
Voice (303)315-7967 Fax (303)315-8026
Email Terry.Howard@UCHSC.edu
=========================================================================
Date: Fri, 12 Feb 1999 13:08:12 EST
Reply-To: Karen=B.=Byers%SS%DFCI@EYE.DFCI.HARVARD.EDU
Sender: A Biosafety Discussion List
From: "Karen B. Byers"
Subject: Re: Health Sciences Center Design
MIME-Version: 1.0
Content-type: text/plain; charset=us-ascii
How wonderful to be able to design a new health sciences center. I can't help
you with actual examples, but, a reference resource is on the web:
Choose "planning", then a screen pops up with choices which include "NIH
Design Criteria". This is a huge reference may be helpful to you later -- it
describes standard safety criteria in terms intellible to architects and
engineers. Unfortunately, the really difficult part of the process is
getting what was agreed on in the planning stages reflected in the actual
plans...good luck!
=========================================================================
Date: Sat, 13 Feb 1999 19:17:55 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Gergis, Nasr N."
Subject: Re: zoonotics
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Jennifer:
I beleive you register a facility for working with bihazard to ensure the
safety, no matter this facility has vet clinic or not, as you do with any
other biological and/or animal.
-----Original Message-----
From: Jennifer Reader [mailto:jennifer@EHS.UOGUELPH.CA]
Sent: Friday, February 12, 1999 8:22 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: zoonotics
Dear group,
I would like to know what other institutions are doing about
zoonotics.
Currently, we register projects where people are cooking up
things as cultures. We do not register projects involving
potentially biohazardous material such as dead squirrels.
Do you register projects for such things as dead squirrels, live
turtles, healthy chickens (which may have salmonella), etc?
Keep in mind we have a vet teaching hospital so anything can
show up at the door there too.
If you register the project, is it in a different format than
conventional, lab-based, culture work?
Thanks. As you can see, this can be "loaded" politically.
Jennifer Reader, B.S., M.S.P.H.
Hazardous Materials Safety Officer
Environmental Health and Safety
University of Guelph
Guelph, Ontario N1G 2W1 Canada
519-824-4120 X3190 Fax 519-824-0364
e-Mail jennifer@ehs.uoguelph.ca
=========================================================================
Date: Sun, 14 Feb 1999 23:22:03 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: LSW's Laboratory Safety Guidelines
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
LSW's "Laboratory Safety Guidelines: 40 Suggestions for a Safer Laboratory"
are now available online at on the services page.
The "Guidelines" were written in 1976 and distributed by Dow Chemical to 2,000
colleges and universities. Within one year Dow received requests for 250,000
reprints. Today, more than two million copies have been distributed.
Carolina Science and Math, Inc. is assisting LSW in preparing a 20th
Anniversary edition of the "Guidelines" to celebrate LSW's 20th year of
service safety in science education. Poster size copies should be available
for the NSTA national meeting in Boston this coming March. ... jim
*****************************************************
James A. Kaufman, Director
The Laboratory Safety Workshop
Safety in Science Education
192 Worcester Road, Natick, MA 01760
508-647-1900 Fax: 508-647-0062 labsafe@
LSW is a national non-profit educational organization dedicated to making
health and safety an integral and important part of science education. Free
copies of our Laboratory Safety Guidelines, seminar schedule, Publications
List, AV-Lending Library List, and membership information are available on
request. The LABSAFETY-L discussion list is a public service of LSW.
******************************************************
=========================================================================
Date: Tue, 16 Feb 1999 09:59:31 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: zoonotics
MIME-Version: 1.0
Content-Type: text/plain
Hi, Jennifer -
At UCSF, we require "registration" (through our Biological Use
Authorization process) of all research involving, among other things,
research using sheep (because of the risk of Q-fever in our confined
urban setting) and Old World primates (because of cercopithecine
herpesvirus type 1 [the old B virus], SIV, STLV, foamy agent, etc.).
While there are other zoonoses we are concerned about, we cover them in
Animal Care Facility safety training for the staff but don't require
authorization for them. This may seem an excessively conservative
approach but what the hey, we've got to have some conservatism in San
Francisco ...
We use the same process for these authorizations as for rDNA, infectious
agent or toxin work. You can learn more about our process from our
Biosafety manual, available on our web site at .
Hope this helps.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Jennifer Reader [mailto:jennifer@EHS.UOGUELPH.CA]
Sent: Friday, February 12, 1999 5:22 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: zoonotics
Dear group,
I would like to know what other institutions are doing about
zoonotics.
Currently, we register projects where people are cooking up
things as cultures. We do not register projects involving
potentially biohazardous material such as dead squirrels.
Do you register projects for such things as dead squirrels, live
turtles, healthy chickens (which may have salmonella), etc?
Keep in mind we have a vet teaching hospital so anything can
show up at the door there too.
If you register the project, is it in a different format than
conventional, lab-based, culture work?
Thanks. As you can see, this can be "loaded" politically.
Jennifer Reader, B.S., M.S.P.H.
Hazardous Materials Safety Officer
Environmental Health and Safety
University of Guelph
Guelph, Ontario N1G 2W1 Canada
519-824-4120 X3190 Fax 519-824-0364
e-Mail jennifer@ehs.uoguelph.ca
=========================================================================
Date: Tue, 16 Feb 1999 10:09:22 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: Health Sciences Center Design
MIME-Version: 1.0
Content-Type: text/plain
Hi, Terry -
Planning for our new Mission Bay campus is well under way with first
groundbreaking due later this year. Since UCSF is strictly a health
sciences campus and is located in an area, like yours, with a high level
of social and environmental awareness, Mission Bay planning may offer
you some valuable insights. You can get basic info from the UCSF web
site () - just look up Mission Bay in the "A-Z
Listings".
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Terry Howard [mailto:Terry.Howard@UCHSC.EDU]
Sent: Friday, February 12, 1999 9:37 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Health Sciences Center Design
We have the rare opportunity here to redesign our Health Sciences Center
from the ground up. Do any of you out there have suggestions about
which
new facilities, hopefully multidisciplinary Health Science Centers, we
should look at as benchmarks of farsighted, efficient, and effective
design?
We would like a few good examples of state of the art facilities.
Thanks
for any help you can provide.
Terry L. Howard, CIH
Director, Health and Safety Division
UCHSC Department of Facilities Operations
4200 E. 9th Ave., C275
Denver, CO 80262
Voice (303)315-7967 Fax (303)315-8026
Email Terry.Howard@UCHSC.edu
=========================================================================
Date: Tue, 16 Feb 1999 16:20:17 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: CMH Rxn
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hello all,
Is anyone familiar with a cyanomethohemoglobin (CMH) reaction? It is done
in clinical labs to measure hemoglobin. Apparently the waste contains low
levels of both cyanide and blood products. We have a question regarding
wste disposal and disinfection. The haz waste people won't take it because
of the blood. Does anyone know a proper way to disinfect such a waste
product?
Janice Flesher, MS
Principal Industrial Hygienist & Biosafety Officer
Environmental & Occupational Health & Safety Service
University Medical Dental School of New Jersey
97 Paterson Street #227
New Brunswick, NJ 08901
732-235-8497 phone
732-235-8499 fax
fleshejk@umdnj.edu
=========================================================================
Date: Wed, 17 Feb 1999 10:29:28 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Petuch, Brian R."
Subject: FW: CMH Rxn
MIME-version: 1.0
Content-type: text/plain
Content-transfer-encoding: 7BIT
Check out the Omni/Ajax web site omni-. They have a product,
Bioset, that solidifies and disinfects body fluids. It specifically
mentions neutralizing cyanide compounds.
> ----------
> From: Janice Flesher[SMTP:fleshejk@UMDNJ.EDU]
> Sent: Tuesday, February 16, 1999 4:20 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: CMH Rxn
>
> Hello all,
>
> Is anyone familiar with a cyanomethohemoglobin (CMH) reaction? It is done
> in clinical labs to measure hemoglobin. Apparently the waste contains low
> levels of both cyanide and blood products. We have a question regarding
> wste disposal and disinfection. The haz waste people won't take it
> because
> of the blood. Does anyone know a proper way to disinfect such a waste
> product?
> Janice Flesher, MS
> Principal Industrial Hygienist & Biosafety Officer
> Environmental & Occupational Health & Safety Service
> University Medical Dental School of New Jersey
> 97 Paterson Street #227
> New Brunswick, NJ 08901
> 732-235-8497 phone
> 732-235-8499 fax
> fleshejk@umdnj.edu
>
=========================================================================
Date: Thu, 18 Feb 1999 10:21:36 -0500
Reply-To: der@ovpr.uga.edu
Sender: A Biosafety Discussion List
From: Daryl Rowe
Subject: State of the Art Conference on Emerging Diseases
MIME-Version: 1.0
Content-type: text/enriched; charset=US-ASCII
Content-transfer-encoding: 7BIT
0100,0100,0100A State of the Art Conference on Emerging
Diseases, "Veterinary Medicine, Agriculture, and
Human Health," will be held at the University of
Georgia August 13-14, 1999, focussing on new
zoonotic and animal diseases and the facilities
available for study of these diseases. The
conference is designed to coincide with the
opening of the new BSL-3 Animal Health Research
Center at the University of Georgia. There will be a
number of invited speakers addressing various
disease situations, bioterrorism, and biosafety.
Abstracts for additional oral and poster sessions
are currently being solicited. For more information,
visit the web site:
Sincerely,
Daryl
Daryl E. Rowe, Dr.P.H., R.S.
Coordinator for Biosafety
University of Georgia
Boyd Graduate Studies Research Center
Athens, Georgia 30602-7411
(706) 542-0112 E-mail
=========================================================================
Date: Thu, 18 Feb 1999 11:41:20 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Whitehead Job Posting
Mime-Version: 1.0
Content-Type: multipart/mixed; boundary="=====================_919374080==_"
--=====================_919374080==_
Content-Type: text/plain; charset="us-ascii"
Respond to Linda Wolfe -- wolfe@wi.mit.edu if interested in this job.
>Date: 18 Feb 1999 11:12:59 -0500
>From: "Linda Wolfe"
>Subject: Whitehead Job Posting
>To: "Richard Fink"
>X-Mailer: Mail*Link SMTP for Quarterdeck Mail; Version 4.0.0
>
>
>The Whitehead Institute posted this position Feb. 10, 1999. Interested
>candidates should send resumes to: Whitehead Institute for Biomedical
>Research, Human Resources Department, Nine Cambridge Center, Cambridge, MA
>02142.
>
>Associate Safety Officer - Safety Office - Under the direction of the
Director
>of Environmental Health & Safety, will assist with the development,
>implementation and administration of an expanding and complex safety
program.
>Will develop training programs and conduct appropriate safety training for
all
>laboratory, office, service and facilities staff, oversee the chemical and
>biological waste program, perform site inspections for chemical, electrical
>and physical hazards, share emergency response responsibilities (24 hr.
call),
>interface with various consultants and service providers and perform other
>special projects as required. Requires a BA/BS in Environmental Health and
>Safety, Chemistry or related field, a minimum of five years related
experience
>in an academic research environment, knowledge of fire and life safety codes,
>OSHA, EPA and MA DEP regulations, experience handling emergency situations
and
>experience developing written materials and safety training modules. Must
>have strong interpersonal, verbal and written communication skills, be
able to
>prioritize, work independently, as well as in a team and have proficiency
with
>Macintosh based computers and applications. (job #0399)
>
>Since 1990, the Whitehead Institute for Biomedical Research has ranked among
>the top three research institutions in the world in genetics and molecular
>biology. Our Center for Genome Research has become a dynamic leader in
>functional genomics, genome mapping, sequencing, and informatics. As an
>integral part of the MIT teaching community, we attract the brightest
>scientists with promising research agendas, and then give them the freedom to
>pursue their own dreams. The results are nothing short of extraordinary.
>Join us.
>
>We offer excellent salaries, generous benefits (including tuition
>reimbursement, T-pass discount), interaction with the MIT community, state of
>the art research facilities, and a convenient Kendall Square location. Learn
>more at wi.mit.edu.
>
>
>
>
>
>
--=====================_919374080==_
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x-mac-type="42494E41"; x-mac-creator="4D535744"
Content-Transfer-Encoding: base64
Content-Disposition: attachment; filename="Ad for Associate.doc"
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--=====================_919374080==_
Content-Type: text/plain; charset="us-ascii"
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
--=====================_919374080==_--
=========================================================================
Date: Thu, 18 Feb 1999 15:11:37 -0500
Reply-To: rubockpa@UMDNJ.EDU
Sender: A Biosafety Discussion List
From: Paul Rubock
Organization: eohss-umdnj
Subject: gas connection in BSC
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Can anyone supply a reference requiring a hard connection to a
Biological Safety Cabinet's natural gas supply valve. I noted a lab
using about 10 feet of rubber hose, connected to a bench-top gas outlet,
to provide natural gas for the Bunsen burner in the cabinet. I know
this isn't right, but around here citing a specific reg. makes it easier
to get thinks done.
Thank you,
Paul Rubock
=========================================================================
Date: Fri, 19 Feb 1999 08:02:24 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: gas connection in BSC
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Paul:
Local and State codes apply. Most gas companies won't "endorse"
attachment of any burning source to anything with a rubber hose except
for a few feet on the desk top [in the open lab] from a heavy duty approved
hose connection as used in the traditional Burnsen burner to flame out
bubbles in freshly poured agar. etc. As we all know, flaming to achieve
aseptic culture transfer is unnecessary and is an archaic (sp) technique.
Your institution's Safety or Biosafety Committee can establish its
own safety code and force a hard pipe connection that a licensed plumber
would be allowed to legally install.
The bigger question is why does the lab director need gas flaming in the
biosafety cabinet???? Every study done says it causes contamination
problems rather than solving them. Aseptic technique and transfer does
not "School" the use of flaming. If the workers are using flaming to
decontaminate alcohol soaked glass spreaders of bacteria, a small alcohol
lamp will serve to ignite the alcohol and avoid the dangers of improperly
used natural gas in a biosafety cabinet. I believe the cabinet
manufacturer's counsel users that gas burners with high volume flames create
"eddy's" in the cabinets, beside posing a leak problem. Look through
the manufacturer's web sites and I believe you can get recommendations
not to have NG hook ups to cabinets, period!
Joe Coggin, Jr.
USA College of Medicine
Professor and Chair,
Microbiology and Immunology
LMB
Mobile, AL 36688
On Thu, 18 Feb 1999, Paul Rubock wrote:
> Can anyone supply a reference requiring a hard connection to a
> Biological Safety Cabinet's natural gas supply valve. I noted a lab
> using about 10 feet of rubber hose, connected to a bench-top gas outlet,
>
> to provide natural gas for the Bunsen burner in the cabinet. I know
> this isn't right, but around here citing a specific reg. makes it easier
>
> to get thinks done.
>
> Thank you,
> Paul Rubock
>
=========================================================================
Date: Sat, 20 Feb 1999 11:36:55 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Andrew Braun
Subject: Re: gas connection in BSC
MIME-Version: 1.0
Content-Type: text/plain; charset=ISO-8859-1
Content-Transfer-Encoding: 8bit
Dear all,
At last year's ABSA meeting (or was it the year before) I noticed that the
Rockefeller U safety guide says that Bunsen burners are forbidden in
Biosafety cabinets. The gas taps were closed off. Special dispensation was
required for investigators that had a good reason for installing a burner.
Right on!
The use of burners in a Biosafety cabinet is a very poor idea from nearly
every point of view. Here is what we tell our investigators:
C We discourage flaming flask tops in tissue culture hoods (Biosafety
cabinets). As far as I can see flaming under these circumstances is of no
value and may even be counterproductive. Ages ago bacteriologist doing
their work on benches in filthy labs found flaming a useful method of
protecting cultures from airborne spores. Heating the top of a flask
established a rising convection current that deflected falling spores.
While that was probably useful for bench workers in 1960, there are at
least three reasons it makes no sense in 1998 in a Biosafety cabinet.
First, the air in the work area of a Biosafety cabinet has passed through a
HEPA filter and is therefore sterile. There are no spores to deflect.
Second, any convection from the top of a flask will be defeated by the
downward air flow in the cabinet. And finally, it is probably impossible to
heat the top of a plastic flask to useful temperatures without melting it.
C In addition the burner flame distorts the hood s laminar flow thereby
increasing (!) the chance of contamination.
C On the other hand a Bunsen burner in a Biosafety cabinet is dangerous.
There are regular reports of fires and injury from fires in cabinets. In
general the hoses used to supply the burner are flammable (tygon and latex
are flammable). Picture the consequences of a tygon hose catching fire! So
if you feel it is necessary to use a burner in the cabinet please switch to
flame resistant heavy butyl rubber hoses.
Over the years we have been able to convince most investigators that
burner use is foolish. As the years go by resistance has dropped and every
investigator who has stopped using burners reports not change in
contamination (or, occasionally a reduction in contamination problems due
to the shorter time flasks are open).
Andy Braun, Sc.D.
Massachusetts General Hospital
Brigham and Women's Hospital
McLean Hospital
=========================================================================
Date: Mon, 22 Feb 1999 01:34:38 -0500
Reply-To: bozena@
Sender: A Biosafety Discussion List
From: Ladislav Kristof
Subject: Double Standards in H&S at the University of Guelph
Content-Type: text/plain
MIME-Version: 1.0
Content-Transfer-Encoding: 8bit
Dear BioSafety Mailing List
I had been a laboratory technician for many years (now retired) at the
University of Guelph (ONT. Canada) mostly involved with research in areas such
as horticulture and plant Biochemistry. I have always tried to work safely and
throughout my career Health and Safety officials and other university
personnel have always exhibited the greatest of care in ensuring and promoting
the safety of others in University laboratories. However the recent promotion
of a researcher from the Microbiology dept. (Lucy Mutharia) to the chair of the
Biohazards safety committee by the University should have the Health and Safety
community and the public up in arms. Fraud and lack of scientific integrity
are now being rewarded with a promotion to a safety position that was held in
contempt by this very same researcher.
My wife (Bozena) was infected with deadly blood parasite -African Trypanosomes,
endemic to only Africa- at the University of Guelph by Dr. Mutharia who had
conducted her work in my wife's laboratory in the Microbiology department. Dr.
Mutharia had failed to apply the safety procedures required by federal law to
this research and conducted experiments with this infectious material in my
wife's presence and assistance and in a laboratory frequented by undergraduate
students and other staff. Dr. Mutharia lied to my wife and to other staff
concerning the nature of her work and it wasn't until 4 years after my wife's
exposure and infection that she was made aware that she was working with this
pathogen. However even at this point when my wife was already close to death,
Dr. Mutharia claimed her work involved only non-human pathogenic forms of this
parasite and therefore was impossible to contract. Later it was discovered
through documentation obtained by the Freedom of Information Act that Dr.
Mutharia was working with live human pathogenic forms.
My wife was diagnosed and then treated by a leading specialist in
Sherbrooke Quebec for this disease but the University of Guelph and the Ont.
Ministry of Labour covered up the fact that the researcher had been working
with human pathogenic forms and that the researcher had neglected all of the
safety protocols required by law and had purposely neglected to inform the
Biohazards Safety Committee of this research. The result of this was that my
wife was refused any worker's compensation whatsoever and the University made
sure that my wife would receive no medical help for this disease (by sending a
malicious letter across Canada) which has a high chance of relapse if treated
in its later stages as was her case since she was not informed about the
Trypanosomal research. For example, Dr. Keystone, a Toronto tropical medical
doctor/specialist, (and one of the few able to treat this rare disease in
Canada) refused to even test my wife for the disease after discussing her
case with the University of Guelph.
Dr. Mutharia had lied on various occasions concerning the strains she had
worked with especially covering up the fact that she had used T. gambiense and
T. rhodesiense (human pathogens) in her research nor had she ever informed the
Biohazards committee of her intention to work with such strains. This she did
not do since her original permits required her to impose level-2 containment
which she neglected to follow -i.e she conducted her work in an
undergrad. teaching lab. Moreover the Ministry of Labour acknowledged the
potential of more people being infected as did the Prime Minister's office. The
net result is the promotion of such people to a position where they will do
even more damage, forget about human life and deceive/falsify if necessary.
This is corruption at its worst and the University administration by supporting
and rewarding such a person who had almost killed my wife are no less guilty of
the same crime (as is the MOL for helping the University in their cover-up).
for more info see our web site URL at :
sincerely
Ladislav Kristof
=========================================================================
Date: Mon, 22 Feb 1999 12:43:39 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Gerry.Griffin"
Subject: adenovirus and SPF facility
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; CHARSET=US-ASCII
Do other facilities have BSL 2 rooms within specific pathogen free (SPF)
facilities or are these kept totally separate?
In a separate but related question - as we are trying to accomodate an
increasing number of researchers who want to work with replication
defective adenoviral vectors. For those who require that work with
replication defective adenoviral vectors be conducted at BSL 2 - for how
long following treatment with the vector do you require that animals be
housed in BSL2 rooms and bedding changed as if potentially infectious,
etc.
Please feel free to post directly to me. Once again, thanks in advance
for your help.
----------------------------------------
Gerry Griffin
Environmental Services
Email: Gerry.Griffin@med.nyu.edu
=========================================================================
Date: Tue, 23 Feb 1999 08:19:54 +1300
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stuart Macdiarmid
Subject: Double Standards in H&S at the University of Guelph -Reply
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: quoted-printable
I do not think it appropriate for Ladislav Kristof to wage his polemics =
against the University of Guelph and Professor Lucy Mutharia on this =
internationally-distributed discussion list.=20
Stuart MacDiarmid
=========================================================================
Date: Mon, 22 Feb 1999 14:27:41 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Robin Newberry
Subject: IBC forms and submission procedures
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
We've had a complaint about our IBC's forms and procedures. Essentially the
IBC has an 11 page form they require - which is routed to me prior to
submission to the IBC so that I can run down any gross problems - then
mailed to the IBC members who answer (again via mail) with either a thumbs
up, a thumbs down, questions, or a request for a full committee meeting on
the project. If everything goes well, it takes less than two weeks to get
IBC approval (committee members are given 10 days to respond).
Complicating this is the fact that the IBC has taken it on themselves to
review and approve all biohazard protocols and chemical hazard protocols
(the origins of this can be traced back to a time many years ago before
Clemson had a real EHS program). So many folks who would not normally have
to deal with the IBC have to get IBC approval prior to project initiation.
Even though this is not my committee - it comes out of the Provost's
office; I'm merely an ex officio member - my boss wants me to check and see
how others do their IBC submissions. If you have time, could you please
tell me:
1) Do you require a form for IBC submissions?
2) If so, how long is it?
3) How long does it take your IBC to return approval?
4) Does your IBC review protocols other than rDNA?
I've been asked to make suggestions for streamlining our procedures, so any
suggestions you may have would be welcome.
Many TIA,
Robin
W. Robert Newberry, IV CIH, CHMM
Director, Environmental Health and Safety
Clemson University
wnewber@clemson.edu ehs@clemson.edu
=========================================================================
Date: Mon, 22 Feb 1999 14:57:00 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: IBC forms and submission procedures
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hi Robin,
We do require a form for IBC submissions, but it's only one page. Our IBC
meets once a month, so approvals can be made w/in the month if there are no
complications. We also review the biosafety level of pathogens. I could
send you copies of forms if I could have your fax #.
Janice
At 02:27 PM 2/22/99 -0500, you wrote:
>We've had a complaint about our IBC's forms and procedures. Essentially the
>IBC has an 11 page form they require - which is routed to me prior to
>submission to the IBC so that I can run down any gross problems - then
>mailed to the IBC members who answer (again via mail) with either a thumbs
>up, a thumbs down, questions, or a request for a full committee meeting on
>the project. If everything goes well, it takes less than two weeks to get
>IBC approval (committee members are given 10 days to respond).
>
>Complicating this is the fact that the IBC has taken it on themselves to
>review and approve all biohazard protocols and chemical hazard protocols
>(the origins of this can be traced back to a time many years ago before
>Clemson had a real EHS program). So many folks who would not normally have
>to deal with the IBC have to get IBC approval prior to project initiation.
>
>Even though this is not my committee - it comes out of the Provost's
>office; I'm merely an ex officio member - my boss wants me to check and see
>how others do their IBC submissions. If you have time, could you please
>tell me:
>
>1) Do you require a form for IBC submissions?
>2) If so, how long is it?
>3) How long does it take your IBC to return approval?
>4) Does your IBC review protocols other than rDNA?
>
>I've been asked to make suggestions for streamlining our procedures, so any
>suggestions you may have would be welcome.
>
>Many TIA,
>
>
>
>Robin
>
>W. Robert Newberry, IV CIH, CHMM
>Director, Environmental Health and Safety
>Clemson University
>
>wnewber@clemson.edu ehs@clemson.edu
>
>
>
Janice
=========================================================================
Date: Mon, 22 Feb 1999 14:57:29 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Robin Newberry
Subject: Re: IBC forms and submission procedures
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
>Hi Robin,
>
>We do require a form for IBC submissions, but it's only one page. Our IBC
>meets once a month, so approvals can be made w/in the month if there are no
>complications. We also review the biosafety level of pathogens. I could
>send you copies of forms if I could have your fax #.
Bless you.
864-656-7630
Many TIA,
Robin
W. Robert Newberry, IV CIH, CHMM
Director, Environmental Health and Safety
Clemson University
wnewber@clemson.edu ehs@clemson.edu
=========================================================================
Date: Mon, 22 Feb 1999 20:27:04 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Bozena Kristof
Subject: Re: Double Standards in H&S at the University of Guelph -Reply
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
If my statements are slanderous and mere polemics then the University, Dr.
Mutharia and the MOL can take the proper precautions. If what I say is
true then I have a moral and ethical duty to inform the public about such
people/institutions. You have not lived through such an ordeal as my wife
has and I am sure you would feel differently if you did.
sincerely
Ladislav Kristof
On Tue, 23 Feb 1999, Stuart Macdiarmid wrote:
> I do not think it appropriate for Ladislav Kristof to wage his polemics against the University of Guelph and Professor Lucy Mutharia on this internationally-distributed discussion list.
>
> Stuart MacDiarmid
>
=========================================================================
Date: Tue, 23 Feb 1999 21:04:14 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Richard W. Gilpin, Ph.D., RBP, CBSP"
Organization: Johns Hopkins University & Hospital
Subject: Bioterrorism
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
There was a symposium held for two days in Alexandria VA last week
attended by one thousand health care providers, including members of
ABSA. The National Symposium on Medical and Public Health Response to
Bioterrorism was hosted by the Johns Hopkins Center for Civilian
Biodefense Studies. If you happen to work with hospitals or medical
personnel, check out Johns Hopkins' Civilian Biodefense web site
Richard
--
Richard W. Gilpin, Ph.D., RBP, CBSP
Biosafety Officer
Assistant Professor of Medicine (SOM)
Assistant Professor of Environmental Health Sciences (SHPH)
Director, Biosafety Division
Department of Health, Safety & Environment
Johns Hopkins Institutions
2024 East Monument Street, Room 2-700
Baltimore MD 21205-2223
Phone (410) 955-5918
Fax (410) 955-5929
Internet Email: gilpin@welchlink.welch.jhu.edu
Hopkins Groupwise: gilpin@jhmi.edu
=========================================================================
Date: Wed, 24 Feb 1999 09:01:39 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: FRANCIS COLE
Subject: Bioterrorism -Reply
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: quoted-printable
Richard, Is the hopkins- correct address? I can't =
access it. Anyone else having luck? Frank
=========================================================================
Date: Wed, 24 Feb 1999 09:16:51 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Michael R. Betlach"
Subject: Re: Bioterrorism -Reply
MIME-Version: 1.0
Content-Type: text/plain
The site comes up fine for me with the address Richard supplied. (No space
between and hopkins-...)
Michael Betlach
Promega Corp.
Madison, WI
> -----Original Message-----
> From: FRANCIS COLE [SMTP:FCOLE@]
> Sent: Wednesday, February 24, 1999 9:02 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Bioterrorism -Reply
>
> Richard, Is the hopkins- correct address? I can't
> access it. Anyone else having luck? Frank
=========================================================================
Date: Wed, 24 Feb 1999 10:04:27 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Dave Gelpke
Subject: Re: Bioterrorism -Reply
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
got into it at 9:15 AM EST
FRANCIS COLE on 02/24/99 10:01:39 AM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Dave Gelpke/CORP/US/PBC)
Subject: Bioterrorism -Reply
Richard, Is the hopkins- correct address? I can't
access it. Anyone else having luck? Frank
=========================================================================
Date: Wed, 24 Feb 1999 10:44:02 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Norman, Randy"
Subject: Re: Bioterrorism -Reply
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
The URL is correct (
). I have been looking around in there
for about ten minutes now.
Randy Norman
Safety Specialist Sr.
BioReliance Corporation
Rockville, MD 20850
Rnorman@
"Success is a journey, not a destination" - Ben Sweetland
=========================================================================
Date: Wed, 24 Feb 1999 16:10:39 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: FRANCIS COLE
Subject: Re: Bioterrorism -Reply -Reply
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: quoted-printable
got it thanks. Frank
=========================================================================
Date: Wed, 24 Feb 1999 16:47:03 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Michael R. Betlach"
Subject: Second and last call for Safety Training Survey.
MIME-Version: 1.0
Content-Type: text/plain
Thanks to all who have already replied.
> From: Brian Olson
> Sent: Wednesday, February 24, 1999 4:12 PM
> To: 'Safety'; 'LABSAFETY-L Discussion List';
> 'radsafe@romulus.ehs.uiuc.edu'; 'BIOSAFTY@MITVMA.MIT.EDU'
> Subject: Second and last call for Safety Training Survey.
>
>
> If you are using electronic training techniques, PLEASE take a minute to
> fill out the following survey and send to bolson@
> . I will be compiling the results and will
> post to these four lists later this month.
>
> -----Original Message-----
> From: Brian Olson
> To: 'Safety'; 'LABSAFETY-L Discussion List';
> 'radsafe@romulus.ehs.uiuc.edu'; 'BIOSAFTY@MITVMA.MIT.EDU'
> Subject: Inter/Intranet and/or MM Safety Training Survey.
>
>
>
> THIS SURVEY IS BEING SENT TO SEVERAL SAFETY MAIL-LISTS. TO ENSURE YOUR
> SURVEY IS INCLUDED, PLEASE SEND IT TO bolson@
> .
>
> Good day folks. As a reader and (mild) participant of the safety
> mail-lists for the past 5 years, I have a big favor to ask. My company
> is looking at training options. This survey requires mostly one-word
> answers.
>
> The survey looks at ELECTRONIC SAFETY TRAINING OPTIONS. I know this topic
> has been talked about in some of the better known publications, but I am
> interested in the feedback from you all.
>
> I am sending this to four safety mail-lists. To assure your entry is
> tabulated, please send your reply to bolson@
> . I will be tabulating all responses and will
> send them to the RADSAFE, BIOSAFETY, SAFETY, and LABSAFETY mail-lists by
> the end of the month.
>
> If you are using some type of electronic safety training, please take a
> minute and complete the survey. Between the four mail-lists that are
> receiving this request, I feel many could benefit from the results:
>
> 1. What type of institution are you with? (business, academia, large,
> small, other)
> 2. What factor(s) did you consider before/while you moved to electronic
> training?
> (staffing?, location of buildings?, cost?, etc.)
> 3. Does your institution have access to Intranet? Internet? LAN? CD-ROM
> Carousel?
> 4. What type of elec. training medium are you using? (Intranet, Internet,
> LAN, CD-ROM, e-mail),
> 5. List some of the titles you offer: (e.g. lab safety, biosafety,
> radsafety, hazcomm, dot, etc.),
> 6. Do you offer classroom and/or hands-on training in addition to the
> electronic training, or does this replace it?
> 7. Did you purchase pre-made titles or did you create your own?
> 8. If pre-made, what software company(s) did you go with? (Marcom,
> Mastery, Summit, J.J. Keller, other?)
> 9. If created by you, what authoring tools did you use?
> (Director/Authorware, Dreamweaver, Frontpage, etc.)
> 10. Either way, on average, what was your cost per title? Total budget
> (range)?
> 11. On average, how big is your audience - per title? ( 0-50, 51-99,
> 100-299, >300 )
> 12. Do you use electronic training for annual refresher training?
> 13. Is changing content annually (for freshness) important to your
> audience? (i.e. Are you able to change your subject content when you
> want? Change test questions? Both? Neither? )
> 14. Overall, are you happy with your choice to go with electronic
> training? (i.e. are you getting the results you want? high retention,
> etc.)
>
> Thanks for your participation. I will tabulate and post a summary
> analysis of the results (sent to bolson@
> ) by the end of February.
>
> Thanks folks,
> Brian Olson
> Manager, EH&S
> Promega Corp. (is a biotech co.)
> Madison, WI
> bolson@
>
>
=========================================================================
Date: Wed, 24 Feb 1999 17:15:19 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Philip C. Dugger"
Subject: Re: Second and last call for Safety Training Survey.
MIME-Version: 1.0
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On Wed, 24 Feb 1999 16:47:03 -0600 "Michael R. Betlach"
wrote:
>Thanks to all who have already replied.
>
>
>> From: Brian Olson
>> Sent: Wednesday, February 24, 1999 4:12 PM
>> To: 'Safety'; 'LABSAFETY-L Discussion List';
>> 'radsafe@romulus.ehs.uiuc.edu'; 'BIOSAFTY@MITVMA.MIT.EDU'
>> Subject: Second and last call for Safety Training Survey.
>>
>>
>> If you are using electronic training techniques, PLEASE take a minute
to
>> fill out the following survey and send to bolson@
>> . I will be compiling the results and
will
>> post to these four lists later this month.
>>
>> -----Original Message-----
>> From: Brian Olson
>> To: 'Safety'; 'LABSAFETY-L Discussion List';
>> 'radsafe@romulus.ehs.uiuc.edu'; 'BIOSAFTY@MITVMA.MIT.EDU'
>> Subject: Inter/Intranet and/or MM Safety Training Survey.
>>
>>
>>
>> THIS SURVEY IS BEING SENT TO SEVERAL SAFETY MAIL-LISTS.
TO ENSURE YOUR
>> SURVEY IS INCLUDED, PLEASE SEND IT TO bolson@
>> .
>>
>> Good day folks. As a reader and (mild) participant of the safety
>> mail-lists for the past 5 years, I have a big favor to ask. My
company
>> is looking at training options. This survey requires mostly one-word
>> answers.
>>
>> The survey looks at ELECTRONIC SAFETY TRAINING OPTIONS. I
know this topic
>> has been talked about in some of the better known publications, but I
am
>> interested in the feedback from you all.
>>
>> I am sending this to four safety mail-lists. To assure your entry is
>> tabulated, please send your reply to bolson@
>> . I will be tabulating all responses
and will
>> send them to the RADSAFE, BIOSAFETY, SAFETY, and LABSAFETY
mail-lists by
>> the end of the month.
>>
>> If you are using some type of electronic safety training, please take a
>> minute and complete the survey. Between the four mail-lists that are
>> receiving this request, I feel many could benefit from the results:
>>
>> 1. What type of institution are you with? (business, academia, large,
>> small, other) Medical Center, Large
>> 2. What factor(s) did you consider before/while you moved to
electronic
>> training?Staffing, computer accessibility, cost
>> (staffing?, location of buildings?, cost?, etc.)
>> 3. Does your institution have access to Intranet? Internet? LAN?
CD-ROM
>> Carousel? Yes
>> 4. What type of elec. training medium are you using? (Intranet,
Internet,
>> LAN, CD-ROM, e-mail), Intranet
>> 5. List some of the titles you offer: (e.g. lab safety, biosafety,
>> radsafety, hazcomm, dot, etc.), All above
>> 6. Do you offer classroom and/or hands-on training in addition to the
>> electronic training, or does this replace it? Some subgroup specific
>> 7. Did you purchase pre-made titles or did you create your own?
premade with option to author
>> 8. If pre-made, what software company(s) did you go with? (Marcom,
>> Mastery, Summit, J.J. Keller, other?) Synquest
>> 9. If created by you, what authoring tools did you use?
>> (Director/Authorware, Dreamweaver, Frontpage, etc.)
>> 10. Either way, on average, what was your cost per title? Total
budget
>> (range)?
>> 11. On average, how big is your audience - per title? ( 0-50, 51-99,
>> 100-299, >300 ) 8500
>> 12. Do you use electronic training for annual refresher training? Yes
>> 13. Is changing content annually (for freshness) important to your
>> audience? (i.e. Are you able to change your subject content when
you
>> want? Change test questions? Both? Neither? ) Both
>> 14. Overall, are you happy with your choice to go with electronic
>> training? (i.e. are you getting the results you want? high retention,
>> etc.) For the buck it's the best option- hands on is preferred but not
feasible
Please remember to post results with HealthcareSafety@
>>
>> Thanks for your participation. I will tabulate and post a summary
>> analysis of the results (sent to bolson@
>> ) by the end of February.
>>
>> Thanks folks,
>> Brian Olson
>> Manager, EH&S
>> Promega Corp. (is a biotech co.)
>> Madison, WI
>> bolson@
>>
>>
=========================================================================
Date: Thu, 25 Feb 1999 16:58:04 +0100
Reply-To: doblhoff@edv2.boku.ac.at
Sender: A Biosafety Discussion List
From: DOBLHOFF-DIER OTTO
Organization: Universitaet fuer Bodenkultur Wien
Subject: Advanced EU Biosafety Workshop
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Dear Biosafety Netters
I would like to draw your attention to the
EU Advanced Practical Workshop on
Biological Safety in Industrial and Agricultural Biotechnology
22 to 25 March 1999
TNO Nutrition and Food Research Institute
Zeist, the Netherlands
Course Program:
Monday, 22. March 1999
Biological Hazards
Hazards, risks and safety
Biological agents
Ecology and pathogenicity
Gentically modified MO
Genetics, host-vector systems, gene products
Transgenic plants
Transgenic animals, insects
Hazard evaluation practical workshop
Tuesday, 23. March 1999
Containmnet of biological hazards
Hazard analysis
Physical containment and plant design
Physical containment process equipment
Biosafety cabinets, demo
Transport of hazardous biological materials
Safety and containment measures practical workshop
Wed., 24. March
Excursions:
Excursion to biopharmaceutical operations, transgenic animal
research and trasgenic plant research facilities
Thursd., 25. March 1999
Legistlation and Guidelines
Tasks and responsibilities: education and training
Legistlation on occpational safety and biosafety
Legistlation on environmental protection and biosafety
EU directives
National guidleines and laws
Information and data management
View of environmental/consumer organisation on biotechnology
Deliberate release simulation practical workshop
For more information please contact
Contact: Mrs. H.B. Bastiaanse, TNO Nutrition and Food Research
Institute, Utrechtweg 48, P.O. Box 360, NL-3700 AJ Zeist, the
Netherlands, tel: 0031-30-6944714, Fax: +31-30-6944192, email:
bastiaanse@voeding.tno.nl
______________________________________________________
______________________________________________________
Otto Doblhoff-Dier
Chairman Working Party Safety in Biotechnology of the European Federation =
Biotechnology
Inst. Appl. Microbiol, Univ. Agric.,
Nussdorfer L=E4nde 11, A-1190 Vienna, Austria, Europe
Tel: *43-1-36006-6204 Fax:*43-1-3697615
EMAIL: doblhoff@edv2.boku.ac.at
WWW:
=========================================================================
Date: Fri, 26 Feb 1999 15:18:27 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Robin Newberry
Subject: Biosafety graphic
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I'm updating my general Biosafety lecture slides and was wondering if
anyone had some neat graphics (preferably color drawings) that they would
share. I'll be glad to share mine, but they were all downloaded off the web
and are freely available, so they might not be of much use.
Many TIA,
Robin
W. Robert Newberry, IV CIH, CHMM
Director, Environmental Health and Safety
Clemson University
wnewber@clemson.edu ehs@clemson.edu
=========================================================================
Date: Sat, 27 Feb 1999 08:41:21 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Winston Longue
Subject: Registration of Microbes.
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I'm currently starting up a registartion of microbes at a research
institute. As I am new to this biological safety role, I'm not sure of what
may be neccessary to be registered under the Biological Safety Officer.
As far as registration is concerned, only potential pathogens are
registered currently.
My question is: Is it neccessary to register E.coli K12 and other strains
of E.coli(which come with test kits and are harmless)? What about
Saccharomyces cerevisiae?
Can anyone comment on this and email me at mcbwel@imcb.nus.edu.sg
Regards,
Winston Longue
Winston Longue
Safety Officer
Institute of Molecular and Cell Biology
=========================================================================
Date: Fri, 26 Feb 1999 22:57:40 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: Free Student Training
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The Northeastern Section of the American Chemical Society will sponsor a free,
one-day lab safety training seminar for undergraduates and graduate students
at UMASS Boston on March 17th.
For more information, contact John Warner at UMASS Boston or the Lab Safety
Workshop. ... Jim
*****************************************************
James A. Kaufman, Director
The Laboratory Safety Workshop
Safety in Science Education
192 Worcester Road, Natick, MA 01760
508-647-1900 Fax: 508-647-0062 labsafe@
LSW is a national non-profit educational organization dedicated to making
health and safety an integral and important part of science education. Free
copies of our Laboratory Safety Guidelines, seminar schedule, Publications
List, AV-Lending Library List, and membership information are available on
request. The LABSAFETY-L discussion list is a public service of LSW.
******************************************************
=========================================================================
Date: Sat, 27 Feb 1999 12:42:35 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Winston Longue
Subject: Biosafety Level.
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I'm interested to know more about the Biosafety Levels of Drosophila
melanogaster and the biological safety requirements for recombinant DNA
work dealing with such organisms. Where can I refer to for a
reference/website for work with drosophila?
Regards,
Winston Longue
Safety Officer
Institute of Molecular and Cell Biology,
National University of Singapore.
mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Sat, 27 Feb 1999 10:28:54 -0500
Reply-To: dliberma@
Sender: A Biosafety Discussion List
From: dan liberman
Subject: Re: Double Standards in H&S at the University of Guelph -Reply
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Stuart Macdiarmid wrote:
>
> I do not think it appropriate for Ladislav Kristof to wage his polemics against the University of Guelph and Professor Lucy Mutharia on this internationally-distributed discussion list.
>
> Stuart MacDiarmid
But it is ok for three people to respond to Francis Cole's comment about
a url and the three responses to advise him that the url is correct and
his public acknowledgement of same. I am more interested in hearing
about a safety fraud which can be verified verses a discussion on url's.
Clearly, his was a cry for help from those he thought would be
sympathetic. Perhaps in the future it might be you who needs the support
of colleagues.
If he is wrong let them sue him. If he is correct then he has
experienced what everyone of us in Biosafety fears most. An investigator
who works with very dangerous agents, covers it up and lies about it in
the process.
Is this an example of bioterrorism from within????
Dan Liberman
=========================================================================
Date: Sat, 27 Feb 1999 13:03:03 -0500
Reply-To: dliberma@
Sender: A Biosafety Discussion List
From: dan liberman
Subject: no list
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BIOSAFTY NO LIST
=========================================================================
Date: Sat, 27 Feb 1999 13:20:05 -0500
Reply-To: dliberma@
Sender: A Biosafety Discussion List
From: dan liberman
Subject: SET BIOSFAFTY-L NOMAIL
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BIOSAFTY-L NOMAIL
=========================================================================
Date: Mon, 1 Mar 1999 08:25:20 +0100
Reply-To: doblhoff@edv2.boku.ac.at
Sender: A Biosafety Discussion List
From: DOBLHOFF-DIER OTTO
Organization: Universitaet fuer Bodenkultur Wien
Subject: slides for lectures
In-Reply-To:
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Date sent: Fri, 26 Feb 1999 15:18:27 -0500
Send reply to: A Biosafety Discussion List
From: Robin Newberry
Subject: Biosafety graphic
To: BIOSAFTY@MITVMA.MIT.EDU
Dear Robert,
I have put a number of my lectures concerning various aspects of
biosafety as PowerPoint files on our web server. Some graphics, a
bit of useful information. Please just beware of the fact, that some
of the graphics have been scanned from publications or have been
downloaded from the internet, so they can not be used for other
publications without the consent of the publishers.
newdev.zip
a lecture on new developements in biosafety including animal cell
cultures (operator and environmental safety, product safety),
prions, emerging infetctious diseases originally presented at the
Medical Faculty in Lublijana 96
prions.zip
a lecture on prions including aetiology, animal and human prion
diseases, inactivation of prions etc. These slides are a subset of
the newdev.zip file
cellcult.zip
a lecture on biosafety of animal cell cultures (operator and
environmental safety) and cell culture derived products (including
virus removal validation) originally presented at the biosafety
workshop in Rio de Janeiro 96
Otto Doblhoff-Dier, Inst. Appl. Microbiol, Univ. Agric.,
Nussdorfer L=E4nde 11, A-1190 Vienna, Austria, Europe
Tel: *43-1-36006-6204 Fax:*43-1-3697615
EMAIL: doblhoff@edv2.boku.ac.at
WWW:
=========================================================================
Date: Mon, 1 Mar 1999 16:08:35 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Winston Longue
Subject: Biosafety Levels
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>Date: Mon, 01 Mar 1999 14:11:58 +0800
>To: A Biosafty Discussion List
>From: Winston Longue
>Subject: Biosafety Levels
>
>To the list,
>
>I'm trying to find out how I can go about sourcing the Biosafety Level of
a particular strain of a microorganism. Does anyone know of a good website
or reference literature. Pls enloghten as I am new to this biological
safety role.
>
>Regards,
>
>
Winston Longue
Safety Officer
Institute of Molecular and Cell Biology,
National University of Singapore.
mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Mon, 1 Mar 1999 11:23:54 -0500
Reply-To: rubockpa@UMDNJ.EDU
Sender: A Biosafety Discussion List
From: Paul Rubock
Organization: eohss-umdnj
Subject: respirators/non-human primates
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Our vivarium will be using non-human primates for the first time. They
will be macaques and while we're already OK vis-a-vis precautions for
Herpes B, another question has come to mind. That is, what do you do at
your institutions regarding TB risk? We'll be testing them upon arrival
and 2X more times afterward. But, I was wondering if there was any
consensus on the need for respirators (N-95) prior to or despite a
negative test. What I'm lacking is a feel for the extent of endemic TB
infections in macaques. Would testing (and the finding of a negative
result) of the animals by the vendor, influence the decision.
Thank you for your response.
Paul Rubock,
UMDNJ
=========================================================================
Date: Mon, 1 Mar 1999 09:38:47 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Serum Sample Banking
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Dear Fellow BIOSAFTY-netters -
We, a subcommittee of the UCSF Advisory Board on AIDS and Emerging
Infections, are in the process of establishing a policy for UC San
Francisco (a medical sciences campus) regarding serum sample banking,
also known as baseline serum sample collection. As you know, there are
pros and cons here and discussions of this can become heated.
The BMBL (1993) and the NIH rDNA guidelines (1998) recommend serum
sample banking as a Special Practice for BSL2, 3 and 4. The Canadian
guidelines (1996) require it for BSL3 and 4. The WHO Lab Safety Manual
(1993) says "should" collect baseline samples for BSL3 and "may" for
BSL1 and 2.
On the other side of the coin, there are those who say that baseline
serum samples are of very limited use. The intent is to have a
comparison specimen in the event of a lab exposure that may result in an
infection and seroconversion. If storage conditions aren't ideal,
attempts to identify immunologic components of the serum after months to
years of storage can yield misleading results. In addition, if the
exposure is reported immediately (as it should be) and not only after
symptoms develop, a baseline sample collected immediately following
exposure would yield the same information but without the possibly
confounding variable of storage.
Then there are the more contemporary concerns about security, anonymity
and other discomforting legal aspects of serum sample banking. Who will
physically hold the samples and how will they ensure that the samples
are labeled so that the donor can't be easily identified? Who will
control the identification key? - the PI, employee health, department
chair, human relations? Who will provide storage space and ensure
stable storage conditions and proper disposal of the samples on a
regular basis? Who will have access to the samples and under what
conditions? Who accepts liability for medical decisions made on the
basis of false positive or negative results associated with the baseline
samples? These are thorny questions. We've all heard horror stories
about how the system has caused problems. Unfortunately, when the
system wroks as intented, we rarely hear about it.
To help us sort out the issues and develop policy that best serves our
campus community, I would appreciate knowing your your own policy and
your thoughts about this. Thanks in advance for your help.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
=========================================================================
Date: Mon, 1 Mar 1999 17:08:22 -0500
Reply-To: vis@uhs.umass.edu
Sender: A Biosafety Discussion List
From: Valerie Seinberg
Organization: EH & S
Subject: HIV and periodic Serum Samples and Testing
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We have a group of virologists who will be working with HIV - (the first
for our campus) and we are in the process of establishing procedures for
baseline serum samples and HIV testing. A few questions for those of
you with experience with HIV at your institutions:
1. Do you collect and store baseline serum samples?
2. Do you require HIV testing before research begins?
3. Do you collect periodic serum samples and test routinely for HIV or
do you only test in case of an exposure?
Thanks,
Valerie
--
Valerie I. Steinberg, PhD CIH CBSP
Environmental Health & Safety
Univ. of Massachusetts
Amherst, MA 01003
(413) 545-2682 Fax (413)545-2600
=========================================================================
Date: Mon, 1 Mar 1999 19:37:13 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Andrew Braun
Subject: Re: HIV and periodic Serum Samples and Testing
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Dear Valerie,
The answers at MGH, The Brigham and Women's Hospital and, I believe,
Harvard, are 1) No, 2) No, and 3) in case of exposure.
It is the opinion of our Infectious Disease people that there is no
clinical benefit to baseline samples. A sample taken immediately after
exposure is probably useless but it is reassuring to the victim. A positive
HIV serology means only one thing no matter what the baseline. The proper
response to an exposure is outlined in last year's CDC guidelines. We have
been told by lawyers that only stored "baseline" samples with a legally
acceptable provenance can be used to benefit a victim or the institution.
This is extremely unlikely in an educational institution. None of us can
figure out how the baseline sample can benefit the victim. It can benefit
the institution. Thus only a fool would voluntarily give their blood to
their institution. Aside from the HIV issue a blood sample can be used
against a donor for other proceedings, such as prescription drug use
(tranquilizers) and drugs one would rather not allow others to know of
(abortion pill, for instance).
A couple of years ago I asked members of ABSA to send me instances of
stored serum use. I have had several responses. All were negative. No one
came up with a use. My opinion is serum storage makes us feel we are doing
something good. It benefits PI's and Biosafety officer's psyches. It is of
no value to victims and or employees. It may help employers in suits
against employees.
Andy Braun
----------
> From: Valerie Seinberg
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: HIV and periodic Serum Samples and Testing
> Date: Monday, March 01, 1999 5:08 PM
>
> We have a group of virologists who will be working with HIV - (the first
> for our campus) and we are in the process of establishing procedures for
> baseline serum samples and HIV testing. A few questions for those of
> you with experience with HIV at your institutions:
>
> 1. Do you collect and store baseline serum samples?
>
> 2. Do you require HIV testing before research begins?
>
> 3. Do you collect periodic serum samples and test routinely for HIV or
> do you only test in case of an exposure?
>
> Thanks,
> Valerie
> --
> Valerie I. Steinberg, PhD CIH CBSP
> Environmental Health & Safety
> Univ. of Massachusetts
> Amherst, MA 01003
> (413) 545-2682 Fax (413)545-2600
=========================================================================
Date: Mon, 1 Mar 1999 22:41:11 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Diane Fleming
Subject: Gene therapy vectors
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I just got the copy of the Feb. 17th Federal Register with the NIH RAC notice
and proposed actions. The risk assessment of adeno associated virus (AAV) is
worth reading. I was pleased to see the analysis of why it was put into RG1.
Check it out.
There will be a discussion of containment criteria for viral vectors at the
3/11-12 meeting of the RAC. I think the outcome will be of great interest to
many of us. In the interim, could those of you who are involved in gene
therapy protocol evaluation let me know how the adenoviral vectors with their
various deletions are being assessed and contained at your facility? For
example, an Adeno 5 vector with one deletion (E1) is being handled in small
quantities using a BSC, and the PI believes that he is using BSL1 containment.
As a result of this attitude, coworkers who must use larger quantities think
they should be able to handle it at BSL 1-LS. I have been told that adenoviral
vectors are handled at BSL 3 in France. Wild type would be BSL2, and I tend to
recommend that. The problem arises with the expense and inconvenience of
BSL-2-LS requirements for scale up to greater than 10 L (250-300L) production
quantities. Should true BSL 2 LS really be required or can the containment be
tailor made? If the latter, what really needs to be done? Your input would be
appreciated. You can send comments directly to me to keep the listserv
uncluttered. (Dimerck@) Thanks, Diane.
Diane O. Fleming, Ph.D., CBSP
Biosafety Consultant
15611 Plumwood Court
Bowie, MD 20716-1434
Tel 301-249-3951
Dimerck@
=========================================================================
Date: Wed, 3 Mar 1999 09:52:34 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Winston Longue
Subject: Thanks for responding.
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Hi fellow boisafty list subscribers,
I would like to thank those who have replied my queries on microbe
registration and biosafety levels of drosophila. It has helped me heaps.
Regards,
Winston Longue
Safety Officer
Institute of Molecular and Cell Biology,
National University of Singapore.
mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Tue, 2 Mar 1999 20:54:37 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: DDT and Malaria
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Please respond directly to Ms. Gray (karegray@rph.health..au) if anyone
can answer the following questions.
Regards,
Edward Krisiunas, MT(ASCP), CIC, MPH
Consultant
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
I wonder wether any of you have any information about the effect of DDT on the
environment and human health.
I am interested in the current debate on the potential use of DDT to control
maleria outbreaks.
Many thanks,
Karen Gray
Royal Perth Hospital
Wellington Street
Perth 6000
Telephone 61-08- 9224 2834
Facsimile 61-08- 9221 2093
=========================================================================
Date: Tue, 2 Mar 1999 22:38:39 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: Recent Grad Seeks Job
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Hi NACHOs, SAFETYs, and BIOSAFETYs,
I met a student, Stephen Flynn, last year at Keene State. He
has recently completed his studies and is looking for....
"I've thought long an hard about a career choice and have firmly
decided that laboratory safety is where I belong. I've always felt at
home with labs and scientists. The unique combinations of degrees and
an interest in toxicology, health and safety seems to make me rather
well suited, and I know that I'd find such a position, and the
associated people extremely stimulating." ....
"I've graduated in December with a BS in Safety Studies to add to my
former BS in Chemistry/Geololgy. I'm trying to find a "particular"
job. The job that I'm looking for is an entry level or somewhat
experienced (I have a couple of years of IH experience) laboratory
safety specialist."
Please contact Stephen directly if you have information about a
position for which he might apply in the New England Area.
Stephen Flynn, 42 Pine Street, Keene, NH 03431 603-352-1850
jsflynn@
*****************************************************
James A. Kaufman, Director
The Laboratory Safety Workshop
Safety in Science Education
192 Worcester Road, Natick, MA 01760
508-647-1900 Fax: 508-647-0062 labsafe@
LSW is a national non-profit educational organization dedicated to making
health and safety an integral and important part of science education. Free
copies of our Laboratory Safety Guidelines, seminar schedule, Publications
List, AV-Lending Library List, and membership information are available on
request. The LABSAFETY-L discussion list is a public service of LSW.
******************************************************
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Date: Thu, 4 Mar 1999 15:32:01 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Winston Longue
Subject: Biosafety requirement for primary human cell line.
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To the list,
I'm curious to know if there is any biosafety requirement with the use of
primary human cell lines derived from the heart. Some molecular work
involving gene knockout will be involved but there is no expression of
toxic genes or metabollite. From what I have been reading, as long as
nothing potentially pathogenic is expressed in the cell line there isn't a
biosafety requirement(primary cells die very quickly anyway)
Must BSL1 practices be observed. Anyone with knowledge on this, please feel
free to enlighten me. Thanks!
Regards,
Winston Longue
Safety Officer
Institute of Molecular and Cell Biology,
National University of Singapore.
mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Thu, 4 Mar 1999 15:57:18 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Winston Longue
Subject: Purchase Book!
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Does anyone know where I may obtain a copy of the NIH Guidlines For
Research Involving Recombinant DNA Molecules? I would like to have the
book, if it is purchaseable through the internet?
Thanks.
Regards,
Winston Longue
Safety Officer
Institute of Molecular and Cell Biology,
National University of Singapore.
mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Thu, 4 Mar 1999 20:35:48 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: Re: Purchase Book!
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Winston
You can download the document via internet and the address is:
.
Regards.
YK Wan
Safety Officer
The Chinese University of Hong Kong
At 03:57 PM 3/4/99 +0800, you wrote:
>Does anyone know where I may obtain a copy of the NIH Guidlines For
>Research Involving Recombinant DNA Molecules? I would like to have the
>book, if it is purchaseable through the internet?
>Thanks.
>
>Regards,
>
>
>Winston Longue
>Safety Officer
>Institute of Molecular and Cell Biology,
>National University of Singapore.
>mcbwel@imcb.nus.edu.sg
>
>
>
Please reply when you receive the message. Thank you.
***** Yu Kwan WAN,
***** Safety Officer
***** The Chinese University of Hong Kong
***** Shatin, NT, Hong Kong
***** Email:
***** ulsoykwan@cuhk.edu.hk
***** ulsoykwan@
***** ulsoykwan@
***** ulsoykwan@
=========================================================================
Date: Thu, 4 Mar 1999 09:13:14 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "P. Moravek"
Subject: Re: Biosafety requirement for primary human cell line.
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Hi,
Any human cell lines are BSL1, for starters. Then, depending on other
parameters, they may be classifies upwards of that.
I don't have time to find the reference right now, but believe me I looked it
up myself.
Best wishes.
--Paula Moravek
Winston Longue wrote:
> To the list,
>
> I'm curious to know if there is any biosafety requirement with the use of
> primary human cell lines derived from the heart. Some molecular work
> involving gene knockout will be involved but there is no expression of
> toxic genes or metabollite. From what I have been reading, as long as
> nothing potentially pathogenic is expressed in the cell line there isn't a
> biosafety requirement(primary cells die very quickly anyway)
> Must BSL1 practices be observed. Anyone with knowledge on this, please feel
> free to enlighten me. Thanks!
>
> Regards,
>
> Winston Longue
> Safety Officer
> Institute of Molecular and Cell Biology,
> National University of Singapore.
> mcbwel@imcb.nus.edu.sg
>
=========================================================================
Date: Thu, 4 Mar 1999 08:25:54 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Kyle Boyett
Subject: Re: Biosafety requirement for primary human cell line.
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Winston, Here at UAB we use BSL 2 for all human materials work involving
primary cell culture because we view the material as being potentially
pathogenic. Even though in some cases the chance is slim to none that any
material is hazardous. Because of the principle of Universal Precautions we
have viewed it easier to manage by requiring all human materials (except
established human cell lines certified free of pathogens)to be manipulated
at BSL 2. Hope this helps.
Kyle Boyett
Asst. Director of Biosafety
Occupational Health and Safety
University of Alabama at Birmingham
e-mail- kboyett@healthsafe.uab.edu
Phone- 205-934-2487
** Asking me to overlook a safety violation is like asking me to reduce the
value I place on YOUR life**
-----Original Message-----
From: Winston Longue [mailto:mcbwel@MCBSGS1.IMCB.NUS.EDU.SG]
Sent: Thursday, March 04, 1999 1:32 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Biosafety requirement for primary human cell line.
To the list,
I'm curious to know if there is any biosafety requirement with the use of
primary human cell lines derived from the heart. Some molecular work
involving gene knockout will be involved but there is no expression of
toxic genes or metabollite. From what I have been reading, as long as
nothing potentially pathogenic is expressed in the cell line there isn't a
biosafety requirement(primary cells die very quickly anyway)
Must BSL1 practices be observed. Anyone with knowledge on this, please feel
free to enlighten me. Thanks!
Regards,
Winston Longue
Safety Officer
Institute of Molecular and Cell Biology,
National University of Singapore.
mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Thu, 4 Mar 1999 09:40:47 EDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jennifer Reader
Organization: Environmental Health and Safety
Subject: Re: Biosafety requirement for primary human cell line.
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We treat all human cell lines as infected with all sorts of
nasties regardless of source.
Jennifer Reader, B.S., M.S.P.H.
Hazardous Materials Safety Officer
Environmental Health and Safety
University of Guelph
Guelph, Ontario N1G 2W1 Canada
519-824-4120 X3190 Fax 519-824-0364
e-Mail jennifer@ehs.uoguelph.ca
=========================================================================
Date: Thu, 4 Mar 1999 10:43:36 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Biosafety requirement for primary human cell line.
In-Reply-To:
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Requirements are usually generated by the regulators. In the US, primary
human cells come under the OSHA bloodborne pathogen standard and hence are
considered BL2 material.
Heart tissue may carry a number of potential pathogens, including: Coxellia
spp., Streptrococcus spp., various viruses. Thus, it would seem to be
prudent to treat human material as BL2 unless demonstrated to be free of
pathogens.
>
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Thu, 4 Mar 1999 10:49:30 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Administrative Stuff
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Seems like I picked an interesting time to go away. Some issues that came up:
1) Advertisements -- I think advertisements are fine, IF they are for a
course (and not a product) that is of general interest to the Biosafety
community and the person posting makes clear if they derive any benefit
from the course.
2) Ladislav Kristof -- This is not the best forum for this as we can do
very little if anything to assist. This would seem best to be handled by
the courts.
3) Replying -- please remember that the mail is coming from the Listserv,
not the individual so when you click reply, the reply goes to the WHOLE
list. Some things are best to sent just to the poster, not the whole list.
Please consider whether the reply is of general interest or is rather
specific.
Thanks all,
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
=========================================================================
Date: Thu, 4 Mar 1999 10:15:48 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: FRANCIS COLE
Subject: Biosafety requirement for primary human cell line. -Reply
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If its a primary human cell line it might be contaminated with a baddie, =
suggest universal precautions. Francis
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Date: Thu, 4 Mar 1999 08:35:31 -0800
Reply-To: A Biosafety Discussion List
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From: "Funk, Glenn"
Subject: Re: Biosafety requirement for primary human cell line.
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Winston -
At UCSF, we treat all human cell cultures, including primary cultures,
"strains" and even well-established cell lines like HeLa, as both
invoking our Bloodborne Pathogens health surveillance program and
requiring BSL2 containment.
Our IBC was recently asked to exempt HeLa and other "well-established"
cell lines from this policy. The Biosafety Committee emphatically and
unanimously said "no" to this request. Among many reasons are the
following:
1. Old lines, such as HeLa, have been in extensive use for so long there
has been ample opportunity for contamination of the line within a lab
and cross-contamination between labs. For example, some HeLa cell lines
have been shown to harbor Mason-Pfizer monkey virus, a known pathogenic
retrovirus;
2. HeLa, a cell line derived from human cervical adenocarcinoma, has
been shown to contain the genome of human papillomavirus type 18
(HPV-18), a virus with known oncogenic properties;
3. Human cell lines may harbor as yet uncharacterized, but potentially
infectious, retroviral elements.
Your own institutional policies are driven by many factors, including
regulations and practicality, but the BSO is usually in the unique
position of being able to place candidate policies before the IBC for
approval and implementation. If you feel that certain levels of control
or containment are advisable for the use of human cell cultures, you may
have to initiate a policy through your IBC that provides you and your
institution with that level of control. Chances are the PIs aren't
going to drive that process for you, although many may (and probably
will) adopt such safety practices as part of their lab's SOP anyway.
Good luck.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Winston Longue [mailto:mcbwel@MCBSGS1.IMCB.NUS.EDU.SG]
Sent: Wednesday, March 03, 1999 11:32 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Biosafety requirement for primary human cell line.
To the list,
I'm curious to know if there is any biosafety requirement with the use
of
primary human cell lines derived from the heart. Some molecular work
involving gene knockout will be involved but there is no expression of
toxic genes or metabollite. From what I have been reading, as long as
nothing potentially pathogenic is expressed in the cell line there isn't
a
biosafety requirement(primary cells die very quickly anyway)
Must BSL1 practices be observed. Anyone with knowledge on this, please
feel
free to enlighten me. Thanks!
Regards,
Winston Longue
Safety Officer
Institute of Molecular and Cell Biology,
National University of Singapore.
mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Thu, 4 Mar 1999 12:25:30 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: Biosafety requirement for primary human cell line.
In-Reply-To:
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Dear Friends:
I disagree with the statement below in part. Some years ago ABSA asked me
to contact OSHA and attempt to discover why they made a preliminary
report that human cell lines and second passaged human cells were to be
rated at BSL-1 risk. The statement was apparently drafted by a
temporary OSHA intern.
I was a consultant to OSHA in drafting the BBP Law and testified
before Congress in hearings on the proposed Law. Dr. Susan Harwood, who
headed the drafting for OSHA, was my graduate student years ago. Susan
unfortunately died several years ago from cancer. She was a wonderful
person. She had placed me in touch with several OSHA lawyers to get a
revised report on classing human cell lines to a RG. I reviewed the
revision and we circulated the corrected position statement to ABSA
members the next year.
The bottom line in the final OSHA position paper was that all human
cell lines and passages of fresh tissue cells in vitro were to be regarded as
BSL-2 (RG2 potential pathogen contaminants). Naturally, fresh and frozen
human tissues were considered to require BSL-2 precautions and animals
injected or subjected to human tissues, blood, body fluids, etc. exposure
in the lab were also BSL-2 activities.
I had warned OSHA that many human cell lines could carry BSL-2 or
RG 2 and possibly RG-3 contaminants and I cited a long list of examples.
After many phone calls and re-assignment of the project to permananent
staff, we got the above formal ruling. It was reported in the ASBA
newsletter.
Hence, like several replys to your inquiry, I am certain human cells
lines are to be considered BSL-2 requiring. Some exceptions, according
to OSHA, would be a highly characterized cell line [GSLP] which had been
exhaustively tested for viral and other contaminants [Mycoplasma],
sequestered in the facility at all times under the hands of a single
tissue culturist, and never allowed to be incubated or transferred with
other cells, and then only if a "qualified biosafety specialist or other
qualified scientist-knowledgeable in biosafety signed off on the
activity" could a human cell line be handled at BSL-1 or GLSP.
Hope this helps.
Joe Coggin, Jr. Ph.D., RBP, CBSP
USA, College of Medicine, Dept Micro.
& Immunology, LMB, Mobile, AL 36688
Fax: (334) 460-7269; Phone (334) 460-6314
E mail address: jcoggin@jaguar1,usouthal.edu
On Thu, 4 Mar 1999, P. Moravek wrote:
> Hi,
>
> Any human cell lines are BSL1, for starters. Then, depending on other
> parameters, they may be classifies upwards of that.
>
> I don't have time to find the reference right now, but believe me I looked it
> up myself.
>
> Best wishes.
>
> --Paula Moravek
>
> Winston Longue wrote:
>
> > To the list,
> >
> > I'm curious to know if there is any biosafety requirement with the use of
> > primary human cell lines derived from the heart. Some molecular work
> > involving gene knockout will be involved but there is no expression of
> > toxic genes or metabollite. From what I have been reading, as long as
> > nothing potentially pathogenic is expressed in the cell line there isn't a
> > biosafety requirement(primary cells die very quickly anyway)
> > Must BSL1 practices be observed. Anyone with knowledge on this, please feel
> > free to enlighten me. Thanks!
> >
> > Regards,
> >
> > Winston Longue
> > Safety Officer
> > Institute of Molecular and Cell Biology,
> > National University of Singapore.
> > mcbwel@imcb.nus.edu.sg
> >
>
=========================================================================
Date: Thu, 4 Mar 1999 14:51:40 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Creutzfeldt-Jakob Disease and Biosafety
In-Reply-To:
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This may be propaganda. But it provides good background. I will look for
the other documents.
Bob
>I am a member of CJD Voice, an e-mail discussion group on Creutzfeldt-Jakob
>Disease, that horrendous infectious fatal brain deterioring disease. Most
>members have lost a loved one to CJD or currently have a loved one with CJD.
>Other members are at high risk of getting the disease. In the group we have
>discussed infection control in health care. Along these lines can anyone on
>this list recommend any written literature (booklets, etc) on CJD infection
>control that people can give their medical providers who are unfamilar with
>the disease?
>
>For those on the list not familiar with CJD I have included some basic
>information.
>
>Creutzfeldt-Jakob Disease (CJD) has greater public health consequences than
>the mere number of reported case might lead one to believe because it is not a
>reportable disease in most places, is often misdiagnosed, is infectious and is
>not killed by normal sterilization. CJD is a horrendious infectious fatal
>brain-deteriorating disease for which there is no treatment or cure. It is
>caused by a prion, which is a protein. One strain of CJD (nvCJD, i.e. new
>variant CJD) is linked to bovine spongiform encephalopathy (i.e. Mad Cow
>Disease) in England. CJD is more common than reported. In one study of
>Alzheimer patients 13% when autopsied were found to really have CJD.
>
>In the United States and throughout the world people get CJD through 3 means:
>familial (genetic), sporadic (don't know how) and iatrogenic (through a
>medical procedure such as human pitutitary growth hormones and human
>gonadotrophin fertility treatments received in 1985 and before, contaminated
>surgical equipment and dura mater and cornea transplants.) CJD can take
>decades after exposure for the patient to show symptoms. However, once a
>person shows symptoms their decline is rapid and they die within a year of
>first showing symptoms.
>
>CJD should be considered whenever a patient develops a rapid dementia and
>myoclonus. The initial symptoms are subtle and ambiguous and include insomnia,
>depression, confusion, personality and behavioral changes, strange physical
>sensations, and memory, coordination and visual problems. Rapidly
>progressive dementia and usually myoclonus (involuntary, irregular jerking
>movements) develop as CJD progresses. Also, language, sight, muscular
>weakness, and coordination problems worsen. The patient may appear startled
>and become rigid. In the final stage the patient loses all mental and physical
>functions. The patient may lapse into a coma and usually dies from an
>infection like pneumonia precipitated by the bedridden, unconscious state. The
>duration of CJD from the onset of symptoms to death is usually one year of
>less. A 14-3-3 spinal fluid test is over 95% effective in diagnosing CJD when
>symptoms are present. (For information on the test contact Dr Clarence
>Joseph Gibbs, Jr.; National Institutes of Health, (30l) 496-4821 or 6321.)
>CJD patients often die at home and therefore have home health service and have
>family members as caregivers.
>
>Since early signs of CJD are often psychological, victims will often receive
>psychological treatment as either an in-patient or an outpatient. In
>addition, people, including school-age children of Creutzfeldt-Jakob Disease
>victims who die in their 40s or younger, often require counseling to deal with
>the death. And, then there's the recipients of medical treatments such as
>human pituitary growth hormones and dura mater transplants who are at high
>risk of CJD and the people with a genetic mutation for familial CJD who must
>live with CJD hangiing over their heads. Another group includes people who
>receive withdrawal notices that the blood they or, worst yet, their children,
>received came from a pool which included a donor that died of CJD. While the
>question of whether CJD is passed through blood products is yet to be
>resolved, receiving this type of notice causes great
>anxiety in people.
>
>Also, since normal sterilization methods do no kill the CJD infectious agent
>and, it can therefore be spread by surgical instruments, it is more of a
>danger to public health than mere number of cases would suggest..
>
>Whether CJD is spread by human blood is controversial,. Many people get
>transfusions every year. Pooled blood products are withdrawn as a precaution
>if it is found after the product has been released that a person in the donor
>pool has died of CJD of is at higher risk of contracting CJD. However, by
>this point it has often already been used in humans. Many people such as
>hemophilliacs and Alpha !-Antitrypsin Deficency sufferers use blood products
>constantly to maintain their health and therefore, receive many withdrawal
>notices. In additon, many people get gammaglobulin shots. Also, while the
>controversy remains as to whether CJD can be passed through blood products,
>blood products continue to be used as an ingredient in vaccines such as the
>measles-mumps-rubella, rabies and allergy shots; in InVitro Fertilization
>(IVF) cultures; and in medical test fluids.
>
>Even though people have recieved notices that the blood products they were
>given have been withdrawn due to CJD risk they can still donate blood. Also,
>England recently decided to not use its own people to get blood plasma any
>more and to get blood plasma from other countries due to fears of nvCJD (the
>CJD related to Mad Cow Disease) being transmitted through blood. Yet people
>from England can donate blood in the United States and Canada. At the end of
>this e-mail I have included the U. S. Congressional Mandate requesting the
>Centers for Disease Control to conduct a study to assess whether the CJD
>infectious agent is spread through blood products.
>
>CJD Voice is an e-mail discussion group. Most members have lost a loved one
>to CJD or currently have a loved one with CJD. Other members include people
>who took human pituitary growth hormones as children and therefore are at
>higher risk of getting CJD and people who received notices that the blood
>products their children received were from a pool that included a person who
>died from CJD. It provides support to these people as well as tries to
>increase funding for CJD research so treatments and a cure can be found. The
>CJD webpage has a message board, chat room, links to other websites with CJD
>information and a list of CJD researchers accepting financial contributions.
>
>The address for the CJD Voice Webpage is
>
>Please feel free to visit the website and to refer other people to it.
>
>Feel free to distribute this information to whomever you choose.
>
>DebbieOney@
>
>
>
> The Centers for Disease Control and Prevention (CDC) Creutzfeldt-Jakob
> Disease Program
>
> A Congressional Mandate
>
> In response to concerns that CJD may be transmitted through blood or
> blood products, the U.S. Congress requested that the
> CDC conduct a study to assess whether CJD is a threat to the safety of
> the nation's blood supply. Researchers believe that
> the risk of transmission to humans through blood products is very
> small since there have been no known cases of CJD
> contracted by humans in this manner. However, precautions are being
> taken to assure that the blood supply is safe from this
> infectious agent. Current blood safety policy requires that any blood
> products made from blood donated by a person who
> later develops CJD, or is found to have risk factors for CJD, must be
> withdrawn. Until the question of transmissibility is
> resolved, the availability and the price of blood products will
> continue to be adversely impacted by shortages caused by
> recalls and the destruction of blood products which, to date, has cost
> over $100 million.
>
> The Program: How You Can Help
>
> Because the signs and symptoms of CJD may not develop for up to 30
> years, a person could be infected and not show any
> symptoms during his or her lifetime. Furthermore, there is no
> screening test available for CJD and the only sure way to test
> for CJD is by analyzing brain tissue after death. The CJD program asks
> families of individuals who have received blood
> products to donate brain tissue after their death. The brain tissue
> will be shipped to Stephen J. Armond, M.D., Ph.D.,
> Professor of Neuropathology, University of California, San Francisco
> to test for evidence of CJD. Other brain tissue will be
> stored at CDC in Atlanta for analysis in the future when more is known
> about the cause of CJD.
>
> Participation in the CJD program is voluntary. The CDC is aware that
> the death of a family member is a difficult time to make
> important decisions. Therefore, the CDC is working through your
> regional coordinator, physician, and treatment center staff to
> provide support to you and your family as you discuss brain tissue
> donation. To help with making an informed and rational
> decision, the CDC has developed information packets which answer
> commonly asked questions about the program including:
> how to authorize the donation; how confidentiality will be assured;
> the ability to retrieve the brain tissue without disfigurement
> and without affecting funeral arrangements; and the time frame for
> receiving test results.
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Thu, 4 Mar 1999 16:33:17 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ben Owens
Subject: Biological Sampling of Wet Carpet
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I was contacted by a woman who has a manufactured home that has a
history of water leaks and subsequent wet sub floor (black from mold)
and carpet pad (and presumably wet carpet). To make things worse, she
has a young child that has respiratory problems. She understands that
the recommendation is to fix the leak(s), seal the sub floor, and
replace the pad and carpet. However, the home is under warranty by the
manufacturer and she is anticipating that the manufacturer will be
reluctant to cover the cost of the above recommendation. To provide
"ammo" for her claim the woman would like to have a sample of the carpet
tested for biological agents. My question is what agents should she
have the carpet tested for - mold, fungus, bacteria, all of the above
(specific species?)? How do you interpret positive results from such a
sample? Thanks for your help. Ben
--
Ben Owens, Chemical Hygiene Officer
University of Nevada, Reno
Environmental Health and Safety Department, MS 328
Reno, NV 89557
(702) 327-5196
(702) 784-4553 fax
=========================================================================
Date: Fri, 5 Mar 1999 06:34:45 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: cell cultures & BBP - Purchase Book!
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Dear Winston,
At UT MD Anderson Cancer Center in Houston, TX we handle all viable human
and non-human primate material, using BL2 practices in BL1 or higher
facilities. A quote from the NIH rDNA guidelines Section II-A-3.
Comprehensive Risk Assessment , is pasted below. You don't need to buy the
NIH GUIDELINES FOR RESEARCH INVOLVING RECOMBINANT DNA MOLECULES MAY 1998
and can access the entire document off the web at
"Individuals working with human immunodeficiency virus (HIV), hepatitis B
virus (HBV) or other bloodborne
pathogens should consult Occupational Exposure to Bloodborne Pathogens;
Final Rule (56 FR 64175-64182).
BL2 containment is recommended for activities involving all
blood-contaminated clinical specimens,
body fluids, and tissues from all humans, or from HIV- or HBV-infected or
inoculated laboratory animals."
This is our primary reference. Because it is often difficult to determine
when material
is contaminated with small amounts of blood, etc., and if pathogens in that
blood, may have taken up permanent
residence inside the cells, we apply this guideline to all tissue cultures.
In addition to this ref.,
there have been position letters in the past from OSHA that have indicated
that they
believe that the BBP standard should apply to all human material, including
cell cultures & cell lines.
Our IBC expands this to include non-human primate fluids and cell
lines/cultures, as they felt many
monkey BBPs would present similar risks to humans.
We do not register tissue culture cell lines or track them in any way.
We handle compliance via institution wide training, communication, and
monitoring activities.
Judy Pointer
---------------------- Forwarded by Judy M. Pointer/MDACC on 03/05/99 06:07
AM ---------------------------
Winston Longue on 03/04/99 01:57:18 AM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: Purchase Book!
Does anyone know where I may obtain a copy of the NIH Guidlines For
Research Involving Recombinant DNA Molecules? I would like to have the
book, if it is purchaseable through the internet?
Thanks.
Regards,
Winston Longue
Safety Officer
Institute of Molecular and Cell Biology,
National University of Singapore.
mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Fri, 5 Mar 1999 09:05:07 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ninni Jacob
Subject: Hepatitis A
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Do any of you have specific training for Custodial personnel to deal with
Hepatitis A?
Thanks
Ninni Jacob, CHP
Radiation and Biological Safety Officer
Office of Risk Management
Brown University - Box 1914
164 Angell Street
Providence, RI 02912
Tel:401 863 1738 (direct)
401 863 3353
Fax:401 863 7676
email: Ninni_Jacob@brown.edu
=========================================================================
Date: Fri, 5 Mar 1999 08:50:53 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Biological Sampling of Wet Carpet
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
The mold and fungus definitly. We have had several cases in the Cleveland
area of deaths to infants caused by a "black mold", the genus/species I
cannot remember. I will try to get the name from the MD who identified the
problem and forward it later. this should definitly motivate the
respoonsible parties.
Bob
>I was contacted by a woman who has a manufactured home that has a
>history of water leaks and subsequent wet sub floor (black from mold)
>and carpet pad (and presumably wet carpet). To make things worse, she
>has a young child that has respiratory problems. She understands that
>the recommendation is to fix the leak(s), seal the sub floor, and
>replace the pad and carpet. However, the home is under warranty by the
>manufacturer and she is anticipating that the manufacturer will be
>reluctant to cover the cost of the above recommendation. To provide
>"ammo" for her claim the woman would like to have a sample of the carpet
>tested for biological agents. My question is what agents should she
>have the carpet tested for - mold, fungus, bacteria, all of the above
>(specific species?)? How do you interpret positive results from such a
>sample? Thanks for your help. Ben
>
>--
>Ben Owens, Chemical Hygiene Officer
>University of Nevada, Reno
>Environmental Health and Safety Department, MS 328
>Reno, NV 89557
>(702) 327-5196
>(702) 784-4553 fax
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Fri, 5 Mar 1999 11:52:49 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: black mold
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Our local resident expert is Dr. Dorr G. Dearborn. His webpage, which is
full of this kind of information, is listed below. Hope that this helps.
bob
X-Sender: dxd9@pop.cwru.edu
Date: Fri, 05 Mar 1999 11:26:03 -0500
To: "Robert N. Latsch"
From: "Dorr G. Dearborn"
Subject: Re: Biological Sampling of Wet Carpet
Mime-Version: 1.0
Bob Latsch-
Refer them to our home page which haas
cleanup instructions in addition to links elswhere that may be helpful.
Also, Dr. Linda Stetzenbach at UNLV is a well known expert for them to
contact locally--(702) 895-1419.
Dorr Dearborn
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Fri, 5 Mar 1999 09:19:28 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Alfred L. 'Al' Jin"
Subject: Re: Biological Sampling of Wet Carpet
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Ben,
I would high recommend any comments to this request be done off line.
Because this is matter that does not involve BIOSAFETY but IAQ, it best to
keep out of it since there may be some litigation involved and any
ramifications from such an issue my be detrimental to the future of this
group.
Al Jin, BSO, IH, CBSP, MS, CM(ACM), BSS(ASM), M(ASCP),]
Lawrence Livermore National Laboratory, Livermore, CA,
Hazards Control Department, MS-289,
925 423-7385, E-mail:Jin2@
>I was contacted by a woman who has a manufactured home that has a
>history of water leaks and subsequent wet sub floor (black from mold)
>and carpet pad (and presumably wet carpet). To make things worse, she
>has a young child that has respiratory problems. She understands that
>the recommendation is to fix the leak(s), seal the sub floor, and
>replace the pad and carpet. However, the home is under warranty by the
>manufacturer and she is anticipating that the manufacturer will be
>reluctant to cover the cost of the above recommendation. To provide
>"ammo" for her claim the woman would like to have a sample of the carpet
>tested for biological agents. My question is what agents should she
>have the carpet tested for - mold, fungus, bacteria, all of the above
>(specific species?)? How do you interpret positive results from such a
>sample? Thanks for your help. Ben
>
>--
>Ben Owens, Chemical Hygiene Officer
>University of Nevada, Reno
>Environmental Health and Safety Department, MS 328
>Reno, NV 89557
>(702) 327-5196
>(702) 784-4553 fax
=========================================================================
Date: Sat, 6 Mar 1999 08:39:37 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Winston Longue
Subject: Re: Biological Sampling of Wet Carpet
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hi Ben, as far as fungus and mould is concerned, u should look out for
Aspergillus Mucor spp.. Maybe try a general media for fungus, maybe malt
extract agar or czapek dox agar. Next, isolate pure cultures and look at
its morphology under the microscope. If u think that is what it is, then
try specific agar and identify it by observing its morpholgy. It should
yield a charracterisic morphology on the selective agar.
Hope that helps...
regards,
Winston
At 04:33 PM 3/4/99 -0800, you wrote:
>I was contacted by a woman who has a manufactured home that has a
>history of water leaks and subsequent wet sub floor (black from mold)
>and carpet pad (and presumably wet carpet). To make things worse, she
>has a young child that has respiratory problems. She understands that
>the recommendation is to fix the leak(s), seal the sub floor, and
>replace the pad and carpet. However, the home is under warranty by the
>manufacturer and she is anticipating that the manufacturer will be
>reluctant to cover the cost of the above recommendation. To provide
>"ammo" for her claim the woman would like to have a sample of the carpet
>tested for biological agents. My question is what agents should she
>have the carpet tested for - mold, fungus, bacteria, all of the above
>(specific species?)? How do you interpret positive results from such a
>sample? Thanks for your help. Ben
>
>--
>Ben Owens, Chemical Hygiene Officer
>University of Nevada, Reno
>Environmental Health and Safety Department, MS 328
>Reno, NV 89557
>(702) 327-5196
>(702) 784-4553 fax
>
>
Winston Longue
Safety Officer
Institute of Molecular and Cell Biology,
National University of Singapore.
mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Sat, 6 Mar 1999 10:06:21 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Winston Longue
Subject: Courses/training on biosafety?
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
To the list,
Does anyone know of an institute/university in the US which can conduct
biological safety training. The Institute should follow closely to NIH/CDC
guidelines.I'm new in this biological safety role and I'm looking for a
place where I can visit for a short period for about 2-3wks to learn more
about biological practices in the u.s.
The institute should have a pretty well established biological safety office.
Does NIH/CDC conduct such courses on biological safety training? If so, pls
give me a specific contact person which i could liaise with.
Any help would be greatly appreciated.
Regards,
Winston.
--------------------------------------------------------------------
Winston Longue
Safety Officer
Institute of Molecular and Cell Biology,
National University of Singapore.
mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Sun, 7 Mar 1999 22:55:00 -0500
Reply-To: dliberma@
Sender: A Biosafety Discussion List
From: dan liberman
Subject: Re: respirators/non-human primates
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Paul Rubock wrote:
>
> Our vivarium will be using non-human primates for the first time. They
> will be macaques and while we're already OK vis-a-vis precautions for
> Herpes B, another question has come to mind. That is, what do you do at
> your institutions regarding TB risk? We'll be testing them upon arrival
> and 2X more times afterward. But, I was wondering if there was any
> consensus on the need for respirators (N-95) prior to or despite a
> negative test. What I'm lacking is a feel for the extent of endemic TB
> infections in macaques. Would testing (and the finding of a negative
> result) of the animals by the vendor, influence the decision.
>
> Thank you for your response.
>
> Paul Rubock,
> UMDNJ
Does anyone know if Cynomolgous are more prone to TB than Rhesus or is
the problem the samne in both.
Dan Liberman
=========================================================================
Date: Mon, 8 Mar 1999 04:21:21 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Guy W. Innocente"
Subject: Help on a very basic question
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hello BioSafety group,
I need some help with a basic question.
At what frequency must BioSafety Cabinets (and laminar flow hoods) be
tested and certified on site. I also need to know the regulatory
citation(s) for this.
Due to budget cut-backs I now need to justify the testing of our BSC's,
which has been done semi-annually for as long as I can remember.
I now have to justify this test frequency by showing where this test
frequency is required by OSHA, JCAHO or CAP(for the clinical labs).
Thanks in advance for your help.
Guy Innocente
=========================================================================
Date: Mon, 8 Mar 1999 08:38:17 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Andrew Cockburn
Subject: Re: Help on a very basic question
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
One source is "Primary Containment for Biohazards: Selection, Installation
and Use of Biological Safety Cabinets" by CDC.
There is a copy posted at . See
SECTION VII, Certification of Biological Safety Cabinets.
Andrew Cockburn, PhD
Director of Biological Safety
West Virginia University
Morgantown, WV 26506-9006
Telephone 304-293-7157
Email acockbur@wvu.edu
> -----Original Message-----
> From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
> Behalf Of Guy W. Innocente
> Sent: Monday, March 08, 1999 4:21 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Help on a very basic question
>
>
> Hello BioSafety group,
>
> I need some help with a basic question.
>
> At what frequency must BioSafety Cabinets (and laminar flow hoods) be
> tested and certified on site. I also need to know the regulatory
> citation(s) for this.
>
> Due to budget cut-backs I now need to justify the testing of our BSC's,
> which has been done semi-annually for as long as I can remember.
>
> I now have to justify this test frequency by showing where this test
> frequency is required by OSHA, JCAHO or CAP(for the clinical labs).
>
> Thanks in advance for your help.
>
> Guy Innocente
>
>
=========================================================================
Date: Mon, 8 Mar 1999 09:01:20 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph P. Kozlovac"
Subject: Re: Courses/training on biosafety?
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Winston
Dr. Byron Tepper and Dr. Richard Gilpin of Johns Hopkins Institutes offer a
4.5 day course called "Control of Biohazards In the Research Laboratory".
It is offered in June usually. I do believe they have advertised this
years course on this listserver not to long ago. This may be a good course
for you to attend....I highly recommend it having taken the course myself.
At 10:06 6.3.1999 +0800, you wrote:
>To the list,
>Does anyone know of an institute/university in the US which can conduct
>biological safety training. The Institute should follow closely to NIH/CDC
>guidelines.I'm new in this biological safety role and I'm looking for a
>place where I can visit for a short period for about 2-3wks to learn more
>about biological practices in the u.s.
>The institute should have a pretty well established biological safety office.
>Does NIH/CDC conduct such courses on biological safety training? If so, pls
>give me a specific contact person which i could liaise with.
>Any help would be greatly appreciated.
>
>Regards,
>Winston.
>
>
>--------------------------------------------------------------------
>Winston Longue
>Safety Officer
>Institute of Molecular and Cell Biology,
>National University of Singapore.
>mcbwel@imcb.nus.edu.sg
>
______________________________________________________________________________
Biological Safety Officer
Safety and Environmental Protection Program
NCI - Frederick Cancer Research
and Development Center
(301)846-1451 fax: (301)846-6619
email: jkozlovac@mail.
______________________________________________________________________________
=========================================================================
Date: Mon, 8 Mar 1999 09:05:42 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph P. Kozlovac"
Subject: Re: Help on a very basic question
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
The National Sanitation Foundation Standard 49 recommends biosafety
cabinets be field certified at least on an annual basis. They should also
be certified after major internal repairs, HEPA filter replacement and
when/if the BSC is moved.
At 04:21 8.3.1999 -0500, you wrote:
>Hello BioSafety group,
>
>I need some help with a basic question.
>
>At what frequency must BioSafety Cabinets (and laminar flow hoods) be
>tested and certified on site. I also need to know the regulatory
>citation(s) for this.
>
>Due to budget cut-backs I now need to justify the testing of our BSC's,
>which has been done semi-annually for as long as I can remember.
>
>I now have to justify this test frequency by showing where this test
>frequency is required by OSHA, JCAHO or CAP(for the clinical labs).
>
>Thanks in advance for your help.
>
>Guy Innocente
>
______________________________________________________________________________
Biological Safety Officer
Safety and Environmental Protection Program
NCI - Frederick Cancer Research
and Development Center
(301)846-1451 fax: (301)846-6619
email: jkozlovac@mail.
______________________________________________________________________________
=========================================================================
Date: Mon, 8 Mar 1999 09:18:58 -0500
Reply-To: BBradley@bangate.
Sender: A Biosafety Discussion List
From: Bruce Bradley
Subject: Building BL-3 Lab
MIME-Version: 1.0
Content-type: text/plain; charset=us-ascii
I've been asked to develop some estimates, for budget talks, of the cost of
converting a BL-2 lab to BL-3 and also converting a large animal research
building to BL-3. I know there are many factors which will impact the cost
but I though I would throw this out. Any sources, ideas, or experiences
would be appreciated - like $ per sf :)
**************************************
Bruce D. Bradley, CVM Safety Officer
8401 Muirkirk Road, Laurel, MD 20708
phone 301-827-8252, fax 301-827-8250
bbradley@bangate.
**************************************
*************************************************************************
The opinions and information in this message are those of the
author and do not necessarily reflect the views and policies of
the U.S. Food and Drug Administration. Because of the nature of
electronically transferred information, the integrity or security of
this message cannot be guaranteed.
*************************************************************************
=========================================================================
Date: Mon, 8 Mar 1999 10:38:34 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jairo Betancourt
Subject: Re: Building BL-3 Lab
MIME-version: 1.0
Content-type: text/plain; charset="iso-8859-1"
Content-transfer-encoding: 7bit
Hi Bruce: That is the $ 25,000 question. It is quite complex, but let me lay
some factors, and hopefully the rest of the list will add to it:
1. Ventilation: You want to have a dedicated ventilation for the "new" BL3
lab discharged straight up outside of the bldg., with a HEPA filter in the
exhaust (if your bldg.. is located in a populated area). Obviously with a
negative airflow (how negative?, there are many opinions on this) in
relation to the outside corridors or other areas of the bldg..
2. All cracks, crevices, holes must be sealed. Light and electrical fixtures
should be sealed on the wall.
3. Walls should be sealed with a epoxy coats (how many? up to you and your
facility)
4. Floors ... etc.
etc, etc. If you follow the NIH/CDC Guidelines (BMBl) it will give you an
idea of how much you have to do to upgrade your lab. Sorry about he
generality of my comments. I am sure your will read plenty about it in this
list. Good luck.
-----Original Message-----
From: Bruce Bradley
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Monday, March 08, 1999 10:01 AM
Subject: Building BL-3 Lab
>I've been asked to develop some estimates, for budget talks, of the cost of
>converting a BL-2 lab to BL-3 and also converting a large animal research
>building to BL-3. I know there are many factors which will impact the cost
>but I though I would throw this out. Any sources, ideas, or experiences
>would be appreciated - like $ per sf :)
>
>**************************************
>Bruce D. Bradley, CVM Safety Officer
>8401 Muirkirk Road, Laurel, MD 20708
>phone 301-827-8252, fax 301-827-8250
>bbradley@bangate.
>**************************************
>
>*************************************************************************
>The opinions and information in this message are those of the
>author and do not necessarily reflect the views and policies of
>the U.S. Food and Drug Administration. Because of the nature of
>electronically transferred information, the integrity or security of
>this message cannot be guaranteed.
>*************************************************************************
=========================================================================
Date: Mon, 8 Mar 1999 10:27:45 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Sarah E Palmer
Subject: Biological Weapons list?
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
I am searching for the list of biological agents that is presently being
used for negotiations of the Protocal to the Biological Weapons
Convention. Has anyone heard about or seen this list? I've checked the
Biological and Toxin Weapons Convention web page with no success.
I'd like to see the list so that I can determine how it will impact our
researchers. Any suggestions?
Thanks in advance.
Sarah Palmer, Biosafety Officer
Institutional Biosafety Committee
PO BOX 210106
Life Sciences South, rm 346
University of Arizona
Tucson AZ 85721-0106
spalmer@U.Arizona.EDU
ph. 520 621-5279
=========================================================================
Date: Mon, 8 Mar 1999 14:59:35 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: No Subject
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
I would be interested if someone has a specific protocol for addressing animal
bites occurring to researhers/animal caretakers in research settings (i.e.,
cleaning wound, isolating animal, reviewing immunization history of animal to
determine if there is a concern, etc.).
Regards,
Ed Krisiunas, MT(ASCP), CIC, MPH
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
=========================================================================
Date: Mon, 8 Mar 1999 15:58:32 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Education, Continuing"
Subject: Harvard Lab Design Program
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
The Harvard School of Public Health is pleased to offer the following
program. Please register as early as possible to guarantee your space.
For more information,
please visit;
==================================================
Guidelines for Laboratory Design: Health & Safety Considerations
April 12-16, 1999
Includes guided tours of newly constructed and renovated
laboratories--including clinical, research, classroom, biosafety, and
animal research laboratories.
==================================================
Center for Continuing Professional Education (contedu@sph.harvard.edu)
Harvard School of Public Health
677 Huntington Avenue, LL-23
Boston, MA 02115-6096
Phone: (617) 432-1171
Fax: (617) 432-1969
=========================================================================
Date: Tue, 9 Mar 1999 14:27:04 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: Re: Biological Weapons list?
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
At 10:27 AM 3/8/99 -0700, you wrote:
>I am searching for the list of biological agents that is presently being
>used for negotiations of the Protocal to the Biological Weapons
>Convention. Has anyone heard about or seen this list? I've checked the
>Biological and Toxin Weapons Convention web page with no success.
>I'd like to see the list so that I can determine how it will impact our
>researchers. Any suggestions?
>
>Thanks in advance.
>
>Sarah Palmer, Biosafety Officer
>Institutional Biosafety Committee
>PO BOX 210106
>Life Sciences South, rm 346
>University of Arizona
>Tucson AZ 85721-0106
>
>spalmer@U.Arizona.EDU
>ph. 520 621-5279
>
Sarah
You may search by keyword "bioterrorism"
YK Wan
Safety Officer
Please reply when you receive the message. Thank you.
***** Yu Kwan WAN,
***** Safety Officer
***** The Chinese University of Hong Kong
***** Shatin, NT, Hong Kong
***** Email:
***** ulsoykwan@cuhk.edu.hk
***** ulsoykwan@
***** ulsoykwan@
***** ulsoykwan@
=========================================================================
Date: Tue, 9 Mar 1999 09:00:56 +0100
Reply-To: doblhoff@edv2.boku.ac.at
Sender: A Biosafety Discussion List
From: DOBLHOFF-DIER OTTO
Organization: Universitaet fuer Bodenkultur Wien
Subject: Re: Biological Weapons list?
In-Reply-To:
MIME-Version: 1.0
Content-type: text/plain; charset=ISO-8859-1
Content-transfer-encoding: Quoted-printable
Dear Sarah,
The rolling text of the negotiations with the list of agents can be
found at
[A.] Human Pathogens
[I. Natural organisms]
Viruses
1. Crimean-Congo haemorrhagic fever virus
2. Eastern equine encephalitis virus
3. Ebola virus
4. Hanta viruses98
5. Junin virus
6. Lassa fever virus
7. Machupo virus
8. Marburg virus
9. Rift Valley fever virus
10. [Tick-borne encephalitis virus]99
BWC/AD HOC GROUP/39
Annex 1
Page 118
11. Variola virus (Smallpox virus)
12. Venezuelan equine encephalitis virus
13. Western equine encephalitis virus
14. Yellow fever virus
Bacteria
1. Bacillus anthracis
2. Brucella spp.100
3. Burkholderia (Pseudomonas) mallei
4. Burkholderia (Pseudomonas) pseudomallei
5. [Chlamydia psittaci]
6. Francisella tularensis (tularemia)101
7. Yersinia pestis
Rickettsiae
1. Coxiella burnetti
2. Rickettsia prowazekii
3. Rickettsia rickettsii
Fungi
1. Histoplasma capsulatum (incl. var. duboisii)
[II. Molecular agents]
Toxins
1. Abrin (A. precatorius)
2. Botulinum toxins (Clostridium botulinum)
3. Clostridium perfringens (tox)
4. Corynebacterium diphteriae (tox)
5. Cyanginosins (Microcystins) (Microcystis aeruginosa)
6. [Enterotoxins] [Enterotoxin B] (Staphylococcus aureus)
7. Ricin (Ricinus communis)
8. Saxitoxin (Ganyaulax catanella)
9. Shigatoxin (Shigella dysenteriae)
10. Tetanus toxin (Clostridium tetani)
11. Tetrodotoxin (Spheroides rufripes)
12. Trichothecene mycotoxins [T2]
13. Verrucologen (Myrothecium verrucaria)
14. Aflatoxins
[III. Other agents]
[Prions]
BWC/AD HOC GROUP/39
Annex 1
Page 120
[B.] Animal Pathogens103
The following list of animal pathogens was discussed by the Group
for further consideration with a view to
developing a future list or lists of bacteriological (biological) agents
and toxins, where relevant, for specific
measures designed to strengthen the Convention:
[I. Natural organisms]
1. African swine fever virus
2. Avian influenza virus (Fowl plague virus)
3. [Bluetongue virus]
4. [Camel pox virus]
5. [Classic swine fever virus (Hog cholera virus)]
6. [Contagious bovine (pleuropneumonia)/Mycoplasma mycoides
var. mycoides]
7. [Contagious caprine (pleuropneumonia)/Mycoplasma mycoides
var. capri]
8. Foot and mouth virus
9. [Herpes B virus (monkey)]
10. [Newcastle disease virus]
11. [Peste des petits ruminants virus]
12. [Porcine enterovirus type 9]
13. [Rabies virus]
14. Rinderpest virus (Cattle plague virus)
15. [Sheep pox virus]
16. [Teschen disease virus]
17. [Vesicular stomatitis virus]
18. [African horse sickness virus]
19. [Swine vesicular disease virus]
20. [Lumpy Skin disease virus]
[II. Molecular agents]
[III. Other agents]
[Prions]
[C.] Plant Pathogens
The following list of plant pathogens was discussed by the Group
for further consideration with a view to
developing a future list or lists of bacteriological (biological) agents
and toxins, where relevant, for specific
measures designed to strengthen the Convention:
[I. Natural organisms]
1. [Citrus greening disease bacteria]
2. Colletotrichum coffeanum var. virulans
3. [Chochliobolus miyabeanus]
4. Dothistroma pini (Scirrhia pini)
5. Erwinia amylovora
6. Erwinia carotovora
7. [Phytophthora infestans]
8. Pseudomonas solanacearum105
9. [Puccinia graminis]
10. Puccinia striiformiis (Puccinia glumarum)
11. Pyricularia oryzae
12. [Sugar cane Fiji disease virus]
13. [Tilletia indica]
14. Ustilago maydis
15. Xanthomonas albilineans
16. Xanthomonas campestris pv citri
17. Xanthomonas campestris pv oryzae
18. [Sclerotinia sclerotiorum]
19. [Peronospora hyoscyami de Bary f.sp. tabacina (Adam)
skalicky]
20. [Claviceps purpurea]
[II. Molecular agents]
[III. Other agents]
[Thrips palmi Karny
Frankliniella occidentalis]106
BWC/AD HOC GROUP/39
Annex 1
Page 126
_______________________________________________________
an update to the issue is covered in the quaterly review of
"Progress at the Ad Hoc Group in Geneva, Quarterly Review no 5 "
by Graham S. Pearson
Department of Peace Studies, University of Bradford,UK
to be found on the net under
The new Section I of Annex A is now the
previous II Lists and Criteria (Agents and Toxins). This has been
reor-dered so that there is now a clearer
approach with a list of human, animal and plant pathogens followed
by criteria for human, for animal and for plant
pathogens. In addition some square brackets have been removed
whilst others have been inserted, especially in
respect of animal and plant pathogens which are now entirely
within square brackets for Rinderpest virus. The list
of human pathogens com-prises some 16 viruses, 9 bacteria, 3
rickettsiae, 2 protozoa (within square brackets)
and the list of toxins has reduced from 21 to 19 with the removal of
Aflatoxins and of Ver-rucologen
(Myrothecium verrucaria). The list of animal pathogens has been
reduced from 18 to 14 by the removal of
bluetongue virus, porcine entirovirus type 9, peste des rumi-nants
virus and rabies virus and that of plant pathogens
from 18 to 16 by removal of Erwinia carotovora and Phytophora
infestans. There has been no change to the
subsequent sections of Annex A.
Otto Doblhoff-Dier, Inst. Appl. Microbiol, Univ. Agric.,
Nussdorfer L=E4nde 11, A-1190 Vienna, Austria, Europe
Tel: *43-1-36006-6204 Fax:*43-1-3697615
EMAIL: doblhoff@edv2.boku.ac.at
WWW:
=========================================================================
Date: Tue, 9 Mar 1999 07:37:52 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Sarah E Palmer
Subject: Re: Biological Weapons list?
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Thank you for all of the helpful suggestions. The list I was looking for
is that which Otto posted on the discussion. Another Biosafty netter
provided these helpful US contacts: Dr. Richard Weller, Pacific NW
National Laboratory, 509 372-4838 and Dr. Ronald Atlas, U. Louisville 502
852-0873. In March 1998, they conducted a survey of academia to identify
the potential impacts of POSSIBLE mandated reporting under the Biological
and Toxin Weapons Convention (BWC). The survey was sent to 1500 US
institutions, those with medical and veterinary schools were the primary
recipients.
I spoke to Dr. Weller yesterday and he indicated that the survey results
will be published (not sure where yet) and that both he and Dr. Atlas are
willing to speak to groups (sa ABSA) about their survey and the impact of
the BWC on academia.
Thanks again for all of the help!
Sarah
Sarah Palmer, Biosafety Officer
Institutional Biosafety Committee
PO BOX 210106
LifeSciences South, rm 346
University of Arizona
Tucson AZ 85721-0106
spalmer@U.Arizona.EDU
ph. 520 621-5279
fax 520 621-6159
=========================================================================
Date: Tue, 9 Mar 1999 10:15:00 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Pierre Robillard MD
Subject: 4th ICOH International Conference on Occupational Health for
Health Care Workers
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
De : Pierre Robillard@SSSS le 03/09/99 10:15 AM
Pour : icplist@, biosafty@mitvma.mit.edu, hs-canada@ccohs.ca,
globalocchyg-l@cornell.edu, occ-env-med-l@list.mc.duke.edu,
promed@usa.
cc :
Objet : 4th ICOH International Conference on Occupational Health for
Health Care Workers
THE 4th ICOH INTERNATIONAL CONFERENCE ON OCCUPATIONAL HEALTH FOR
HEALTH CARE WORKERS
Montreal, Canada
Hilton
Bonaventure Hotel
September 28
to
October 1, 1999
SECOND ANNOUNCEMENT, Registration and Call for abstracts is now on our
website
at:
The goal of this international conference is to share solutions to health
and safety
problems facing health care providers. The conference is being held under
the
auspices of the International Commission on Occupational Health.
It is aimed at physicians, nurses, hospital and health care facility
managers,
ergonomists, epidemiologists, infection control professionals, attorneys,
ethicists, etc.
Pierre Robillard MD
Montreal Public Health Department
1301 Sherbrooke street, east
Montreal, Qc, Canada, H2L 1M3
tel.: 514-528-2400 ext. 3245
fax: 514-528-2459
email: probilla@santepub-mtl.qc.ca
=========================================================================
Date: Tue, 9 Mar 1999 11:38:57 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard&Barbara Price
Subject: 4. Biological Weapons list?
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
In reply to Sarah Palmer, here are some of the diseases and toxins being
considered by the BTWC. The list is a draft only.
TOXIN/ BIOREGULATOR
Abrin
Aflatoxins
Anatoxins
Batrachotoxin
Botulinum toxins
Brevetoxins
Bungarotoxins
Centruroides toxins
Ciguatoxin
Cyanginosins/Microcystins
Diphtheria toxin
Endothelin
Modeccin
Palytoxin
Ricin
Saxitoxin
Staphylococal enterotoxins (SEB)
Shigatoxin
Tetanus toxin
Tetrodotoxin
Toxins of Clostridium perfrigens
Trichothecene Mycotoxins (T2, DON, HT2)
Viscumin
Volkensin
HUMAN PATHOGENSVIRUSES
Crimean-Congo HF v.
Eastern Equine Encephalitis (EEE) virus
Ebola virus
[Sin Nombre virus]
[Hantaan virus]
Junin virus
Lassa fever virus
Machupo virus
Marburg virus
Rift Valley fever virus
Tick-borne encephalitis virus
Variola major virus (small pox)
Venezuelan Equine Encephalitis (VEE) virus
Western Equine Encephalitis (WEE) virus
Yellow fever virus
Monkeypox virus
Chikun-Gunya fever virus
Dengue fever virus
Omsk HF virus
HUMAN PATHOGENS (BACTERIA, RICKETTSIAE, PROTOZOA AND FUNGI)
BACTERIA
Bacillus anthracia
Brucella abortus
Brucella melitensis
Brucella suis
Bulkholderia (Pseudomonas) mallei
Bulkholderia (Pseudomonas) pseudomallei
Chlamydia psittaci
Francisella tularensis
Yersinia pestis
RICKETTSIAE
Coxiella birnetti
Rickettsia prowazekii
Rickettsia rickettsii
PROTOZOA
Naegleria fowleri
Naegleria australiensis
FUNGI
Coccidioides immitis
Histoplasma capsulatum
Nocardia asteroides
ANIMAL PATHOGENS
African swine fever virus
Avian influenza virus
Camel pox virus
Classic swine fever virus
Contagious bovine pleuropneumonia (M. mycoides var. mycoides)
Contagious caprine pleuropneumon.
(M. mycoides var. capri)
Foot and mouth virus
Newcastle disease virus
Pest des petits
ruminants virus
Rinderpest virus
Porcine enterovirus type 1
Vesicular stomatitis virus
African hourse sickness v.
Lumpy skin disease virus
PLANT PATHOGENS
Colletotrichum coffeanum var. Virulans
Dothistroma pini (Scirrhia pini)
Erwinia amylovora
Erwinia carotovora
Ralstonia solanacearum
Puccinia graminis
Puccinia striiformiis (Puccinia glumarum)
Pyricularia oryzae
Sugar can Fiji disease virus
Tilletia indica
Ustilgo maydis
Xanthomonas albilineans
Xanthomonas campestris pv citri
Xanthomonas campestris pv oryzae
Sclerotinia sclerotiorum
Peronospora hyoscyami de Bary f.sp. Tabacina (Adam) skalicky
Claviceps purpurea
________________________________________________________________________________
_______
Don't forget to send your completed Who's Who in NBC Defense to ASA
Get the form from
Richard and Barbara Price ....... Applied Science & Analysis, ASA Inc.
PO Box 17533 ....... Portland, ME 04112-8533 ............... USA
phone: 207-829-6376 ............................. fax: 207-829-3040
asa@ .............................
________________________________________________________________________________
________
=========================================================================
Date: Tue, 9 Mar 1999 16:37:41 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Michael R. Betlach"
Subject: Computer Based Training Survey results available
MIME-Version: 1.0
Content-Type: text/plain
Approximately 25 people responded to the survey request. The majority
develop their own in-house training programs (perhaps reflecting that most
respondents were from academic institutions). Those who provided Brian with
survey responses will automatically get a copy of the results.
Others interested in the survey results please see details below to contact
Brian Olson directly.
Michael Betlach, Biosafety Officer
Promega Corp.
> From: Brian Olson
> Sent: Tuesday, March 09, 1999 2:07 PM
> To: 'LABSAFETY-L Discussion List'; 'Safety';
> 'radsafe@romulus.ehs.uiuc.edu'
> Subject: SURVEY RESULTS AVAILABLE: CONTACT ME!
>
> FYI, this is being sent to the SAFETY, RADSAFE, LABSAFETY and BIOSAFTY
> mail-lists.
>
> In early February, I asked you to participate in a Safety CBT (Computer
> Based Training) survey. To those who participated, I THANK YOU FOR YOUR
> TIME - a copy of the survey will automatically be sent out to you today
> (via email).
>
> Anyway, I mentioned that I would send out the results towards the end of
> the month. Thanks for your patience. To save bandwidth, (it is quite
> long) I am asking anyone that is interested in the results of the survey
> to send me an email directly (bolson@). I will email you the
> results.
>
> Thanks all!
>
> Brian Olson
> Manager, EHS
> Promega Corporation (biotech company)
> bolson@
=========================================================================
Date: Wed, 10 Mar 1999 14:05:03 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Winston Longue
Subject: Vaccine required?
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
To the list,
If a research group is working with Yellow virus and its a vaccine strain,
should the personnel working with the virus be vaccinated regardless?
Anyone with such info pls email me at mcbwel@imcb.nus.edu.sg
Regards,
Winston
--------------------------------------------------------------------
Winston Longue
Safety Officer
Institute of Molecular and Cell Biology,
National University of Singapore.
mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Wed, 10 Mar 1999 14:38:52 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Winston Longue
Subject: Database...
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
To the List,
Does anyone know of a reliable database where full information on Biosafety
Levels of microorganisms can be obtained? For example, if I wanted
information on Kunjin virus, strain MRM61C, how do I go about getting such
information, say the safety requirements and whether vaccination is
required....
Any input on this would be greatly appreciated.
Thanks,
Winston
--------------------------------------------------------------------
Winston Longue
Safety Officer
Institute of Molecular and Cell Biology,
National University of Singapore.
mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Wed, 10 Mar 1999 08:01:52 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Heather H. Gonsoulin"
Subject: Re: Database...
MIME-Version: 1.0
Content-Type: multipart/mixed; boundary="---- =_NextPart_000_01BE6ACC.4441FB00"
------ =_NextPart_000_01BE6ACC.4441FB00
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
I would also be very interested in such a resource. Please post answers to the list as I am sure others have the same need.
Heather H. Gonsoulin
Occupational Health and Safety Officer
USL-NIRC
(318) 482-0306 (fax) 318-373-0057
hah8377@usl.edu
-----Original Message-----
From: Winston Longue [SMTP:mcbwel@MCBSGS1.IMCB.NUS.EDU.SG]
Sent: Wednesday, 10 March, 1999 12:39 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Database...
To the List,
Does anyone know of a reliable database where full information on Biosafety
Levels of microorganisms can be obtained? For example, if I wanted
information on Kunjin virus, strain MRM61C, how do I go about getting such
information, say the safety requirements and whether vaccination is
required....
Any input on this would be greatly appreciated.
Thanks,
Winston
--------------------------------------------------------------------
Winston Longue
Safety Officer
Institute of Molecular and Cell Biology,
National University of Singapore.
mcbwel@imcb.nus.edu.sg
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/QD+AMUAIGfG8PWg+cFvYrwAPTFkfj99EQPgcoDKcLIhT7Bp/2Lw+PH9APqxZo9nn2ivFrO1ZQ1L
wqBq9ZHGAHImUEdPsFuh9YJNUk3BYEPdT7BoYrF5UP5hZ/mgvBD/PPF04MjgwQB6cfsiB1VlCZ9z
UU+Q/8UFo/uQcXUdUL+wcKshYgL5UGRRAoN2toD+YzZBZXP+AAdVeHUAkFgo9kFiMPoxcHVRQvH9
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jweCSfHxrIA9APv+8WLwTbSgwnCs8AeRBULeQz1Qz5Bl8bTAZ0+gB1X6TgRWVfKAj9KsgAXQYuH+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------ =_NextPart_000_01BE6ACC.4441FB00--
=========================================================================
Date: Wed, 10 Mar 1999 09:39:45 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Taylor, David G. PHD"
Subject: Re: Database...
MIME-Version: 1.0
Content-Type: text/plain
You might look at the ABSA webpage which has an integrated list of agents.
Individual strains are usually handled at the level as on the general list,
i.e. assumed to be pathogenic. If they are not pathogenic but are used in a
laboratory with other pathogenic strains, then cross-contamination is a
concern.
Dave Taylor
CDC
> -----Original Message-----
> From: hah8377@usl.edu [SMTP:hah8377@usl.edu]
> Sent: Wednesday, March 10, 1999 9:02 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: RE: Database...
>
> I would also be very interested in such a resource. Please post answers
> to the list as I am sure others have the same need.
>
> Heather H. Gonsoulin
> Occupational Health and Safety Officer
> USL-NIRC
> (318) 482-0306 (fax) 318-373-0057
>
>
>
> -----Original Message-----
> From: Winston Longue [SMTP:mcbwel@MCBSGS1.IMCB.NUS.EDU.SG]
> Sent: Wednesday, 10 March, 1999 12:39 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Database...
>
> To the List,
> Does anyone know of a reliable database where full
> information on Biosafety
> Levels of microorganisms can be obtained? For example, if I
> wanted
> information on Kunjin virus, strain MRM61C, how do I go
> about getting such
> information, say the safety requirements and whether
> vaccination is
> required....
> Any input on this would be greatly appreciated.
>
> Thanks,
> Winston
>
>
> --------------------------------------------------------------------
> Winston Longue
> Safety Officer
> Institute of Molecular and Cell Biology,
> National University of Singapore.
> mcbwel@imcb.nus.edu.sg
>
=========================================================================
Date: Thu, 11 Mar 1999 11:14:01 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: medical Surveillance and risk assessments for retrovirus
I will be writing a medical surveillance program for retroviral work. I
have taken the standard wording that seems to be available regarding taking
the employees baseline serum and then perhaps periodically taking more to
test later. If we are using adenovirus, retrovirus, lentivirus: What can
you test for regarding each separately? or what should you test for
regarding each separately? I know you would test for HIV - but what else?
Secondly - I am working on a risk assessment document for retroviral work.
Does anyone have any guidance?
If anyone has guidance reading this please let me know. Thank you!!
Bliss M. Schlank
Biosafety Specialist
Zeneca Pharmaceuticals
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185
Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Thu, 11 Mar 1999 16:06:44 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: Database...
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Winston,
This website in Canada has MSDS's for biologicals. It may not have exactly
what your're looking for but it is a good reference neverless.
At 02:38 PM 3/10/99 +0800, you wrote:
>To the List,
>Does anyone know of a reliable database where full information on Biosafety
>Levels of microorganisms can be obtained? For example, if I wanted
>information on Kunjin virus, strain MRM61C, how do I go about getting such
>information, say the safety requirements and whether vaccination is
>required....
>Any input on this would be greatly appreciated.
>
>Thanks,
>Winston
>
>--------------------------------------------------------------------
>Winston Longue
>Safety Officer
>Institute of Molecular and Cell Biology,
>National University of Singapore.
>mcbwel@imcb.nus.edu.sg
>
>
>
Janice Flesher, MS
Principal Industrial Hygienist & Biosafety Officer
Environmental & Occupational Health & Safety Service
University Medical Dental School of New Jersey
97 Paterson Street #227
New Brunswick, NJ 08901
732-235-8497 phone
732-235-8499 fax
fleshejk@umdnj.edu
=========================================================================
Date: Thu, 11 Mar 1999 16:12:38 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Mike Pirrello
Subject: NBC Incident Resource Page
MIME-Version: 1.0
Content-Type: text/plain
I have tried to put together a resource page for Nuclear/Biological/Chemical
(NBC) Incidents for NC Hazardous Materials Regional Response Team #4. Please
feel free to visit our web site at
and follow the link to the NBC
Incident resource page. At this point, the page is being updated and
expanded almost daily, but hopefully that will slow down in the near future
and I'll be able to improve the formatting a bit.
Constructive criticism and suggestions for additional links and/or
information would be appreciated.
Thanks.
Michael G. Pirrello, CHMM
Team Leader, NC HazMat RRT#4
Durham, NC
Mpirrello@
=========================================================================
Date: Thu, 11 Mar 1999 22:53:03 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: NACHO Lunch
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
The National Association of Chemical Hygiene Officers invites
you to a dutch treat lunch in conjunction with the ACS National
Meeting in Anaheim.
Please join Ray Campbell and Teresa Robertson (two Southern
California NACHOs) for a NACHO lunch at the National ACS
Meeting, on Sunday March 21st, at the West Coast Hotel,
Plaza A, 1855 S. Harbor Blvd., Anaheim, CA.
We will meet at noon directly after the NRCC CHO certification
exam, just outside the exam room.
RSVP to Ray or Teresa (see above)
*****************************************************
James A. Kaufman, Director
The Laboratory Safety Workshop
Safety in Science Education
192 Worcester Road, Natick, MA 01760
508-647-1900 Fax: 508-647-0062 labsafe@
LSW is a national non-profit educational organization dedicated to making
health and safety an integral and important part of science education. Free
copies of our Laboratory Safety Guidelines, seminar schedule, Publications
List, AV-Lending Library List, and membership information are available on
request. The LABSAFETY-L discussion list is a public service of LSW.
******************************************************
=========================================================================
Date: Fri, 12 Mar 1999 09:05:35 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: FW: research-aamc Draft NIH Regulatory Study Available for Commen
t
MIME-Version: 1.0
Content-Type: text/plain
> I just got an email on this this morning and down loaded it. It is a
> large report but I think we all should read it as it may have issues we
> wish to respond to.
>
>
> The House Appropriations Committee report accompanying the FY 1998 NIH
> appropriations bill requested that the NIH undertake a study to
> identify actions that could reduce the regulatory burden on extramural
> researchers. NIH identified five areas for initial study: - financial
> conflict of interest, research integrity, human subjects protections,
> animal care and use, and disposal of hazardous wastes (other than
> radiation). The study included extensive consultations with the
> extramural community, including interviews with extramural scientists
> and research administrators and facilitated workgroups.
>
> The draft report from the NIH study is now available on the web at
> . For the next 30
> days, NIH is seeking comment on the report. Comments should be sent
> to .
>
>
=========================================================================
Date: Fri, 12 Mar 1999 10:09:49 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Erik Talley
Subject: Cadaver Fluid
MIME-Version: 1.0
Content-Type: text/plain
I need some advice about disposal of cadaver fluids from a gross anatomy
lab. The question has arisen after our sister campus sent some "chemical
waste" for incineration but labeled it as "human cadaver waste." In
actuality, 90+% of the waste is phenol, glycerin and formaldehyde used
for embalming or as a wetting agent. As these liquids are collected,
they are stored in a 55G drum for disposal.
When the waste got to the incinerator it was rejected as potentially
infectious since it had "human" in the description. After a lot of
explanation, the disposal company now understands the solution is
chemical waste but says since it has some human waste, they can't accept
it---even though it is preserved human waste therefore falling out of
the standard.
Has anyone else had problems with this or have any suggestions?
Thanks,
Erik
____________________________________
Erik Talley, Assistant Director
Center for Environmental Health and Safety
Southern Illinois University
erik@cehs.siu.edu
=========================================================================
Date: Fri, 12 Mar 1999 11:11:12 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Leslie Delpin
Subject: Re: FW: research-aamc Draft NIH Regulatory Study Available for C
In-Reply-To:
Thanks Terry.
Leslie Delpin - Biological Health and Safety Manager
University of Connecticut
Environmental Health and Safety
3102 Horsebarn Hill Rd. Box U-97
Storrs, CT 06269-4097
(860)486-3613
(860)486-1106
lmdelpin@safety.ehs.uconn.edu
=========================================================================
Date: Fri, 12 Mar 1999 15:28:09 -0400
Reply-To: Thomas Boyle
Sender: A Biosafety Discussion List
From: Thomas Boyle
Subject: News - US Navy Punishes 23
MIME-Version: 1.0
Content-Transfer-Encoding: quoted-printable
Content-Type: text/plain; charset="US-Ascii"
3/12/99 3:25 PM
News - US Navy Punishes 23
Thought you might be interested in viewing this article:
Sorry if you have already saw the information
____________________________________
Thomas Boyle
Biological Safety Officer
Environmental Health and Radiation Safety
University of Pennsylvania
14th Floor Blockley Hall
Philadelphia, Pa. 19104-6021
215 - 898 - 3569 (phone)
215 - 898 - 0140 (FAX)
tom@ehrs.upenn.edu
=========================================================================
Date: Fri, 12 Mar 1999 16:05:08 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Delia Vieira
Subject: autoclave records
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Can anyone tell me how long we have to keep autoclave records for a BSL 3
facility. Any information would be appreciated.
Delia M. Vieira-Cruz
Lab Safety Officer
Albert Einstein College of Medicine
1300 Morris Park Avenue, Forch 800
Bronx, NY 10461
(718)430-3560
vieira@aecom.yu.edu
=========================================================================
Date: Mon, 15 Mar 1999 10:38:08 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Winston Longue
Subject: UV irradiation.
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
To the list,
I was wondering how effective UV light is with regards to uv irradiation of
air-con ducts. Does it reduce significantly the microbial load in the air?
And specificaly what wavelength is recommended.
Anyone with this info, pls feel free to contribute. Thank you!
Regards,
Winston
--------------------------------------------------------------------
Winston Longue
Safety Officer
mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Mon, 15 Mar 1999 07:57:28 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Alan Woodard
Subject: autoclave records -Reply
Mime-Version: 1.0
Content-Type: text/plain
10 NYCRR Section 70-3.5 of the New York State Dept. of Health
Regulations pertaining to the "Operational Requirements For Autoclaves
Used To Treat Regulated Medical Waste" (RMW) requires that records
be maintained for three years.
Discarded infectious materials from BSL 3 laboratories that are
considered zoonotic agents are RMW in New York State.
Alan G. Woodard, Ph.D.
Regulated Medical Waste Program Supervisor
New York State
Department of Environmental Conservation
Division of Solid & Hazardous Materials
50 Wolf Road, Room 206
Albany, New York 12233-7258
Phone: (518) 457-5695
Fax: (518) 485-7723
E-mail: agwoodar@gw.dec.state.ny.us
>>> Delia Vieira 03/12/99 04:05pm >>>
Can anyone tell me how long we have to keep autoclave records for a
BSL 3
facility. Any information would be appreciated.
Delia M. Vieira-Cruz
Lab Safety Officer
Albert Einstein College of Medicine
1300 Morris Park Avenue, Forch 800
Bronx, NY 10461
(718)430-3560
vieira@aecom.yu.edu
=========================================================================
Date: Mon, 15 Mar 1999 09:55:51 PST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: JIM REIMAN
Subject: Re: UV irradiation.
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="ISO-8859-1"; X-MAPIextension=".TXT"
Content-Transfer-Encoding: quoted-printable
Save your money, don't bother. the problem is that you need exposure =
time to get a decent kill, but the air in many ducts moves at at least =
at 1000 feet per minute. so even focused uv has a very short residence/e=
xposure period. There are several charts around listing kill exposures. =
check them out.
Jim Reiman---------
> To the list,
> I was wondering how effective UV light is with regards to uv irradiatio=
n of
> air-con ducts. Does it reduce significantly the microbial load in the =
air?
> And specificaly what wavelength is recommended.
> Anyone with this info, pls feel free to contribute. Thank you!
>
> Regards,
> Winston
>
> --------------------------------------------------------------------
> Winston Longue
> Safety Officer
> mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Mon, 15 Mar 1999 09:26:45 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Madeline J. Dalrymple"
Subject: IBC Project Reviews
MIME-version: 1.0
Content-type: text/plain; charset="iso-8859-1"
Some of our researchers gets grant renewal money to fund a recombinant DNA
research project for years after our IBC initially reviewed the project.
Yet the IBC doesn't hear about the grant renewals. The NIH guidelines
specify IBC review at initiation of a project, but nothing about reviewing
when more money is requested to continue the same research.
Do your IBCs review research for each grant request or by research projects?
Thanks
Madeline
Madeline Dalrymple
Biological Safety Officer
University of Wyoming Environmental Health and Safety Office
Laramie, Wyoming, USA
307-766-2723, fax 307-766-5678
dalrympl@uwyo.edu
=========================================================================
Date: Mon, 15 Mar 1999 11:52:04 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph P. Kozlovac"
Subject: Re: IBC Project Reviews
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
We require our researchers to renew their registrations every three years
for pathogen work and rDNA work. This is in addition to an annual update
letter which we send out to verify that the current information we have on
our database is still correct.
At 09:26 15.3.1999 -0700, you wrote:
>Some of our researchers gets grant renewal money to fund a recombinant DNA
>research project for years after our IBC initially reviewed the project.
>Yet the IBC doesn't hear about the grant renewals. The NIH guidelines
>specify IBC review at initiation of a project, but nothing about reviewing
>when more money is requested to continue the same research.
>Do your IBCs review research for each grant request or by research projects?
>
>Thanks
>Madeline
>
>
>Madeline Dalrymple
>Biological Safety Officer
>University of Wyoming Environmental Health and Safety Office
>Laramie, Wyoming, USA
>307-766-2723, fax 307-766-5678
>dalrympl@uwyo.edu
______________________________________________________________________________
Biological Safety Officer
Safety and Environmental Protection Program
NCI - Frederick Cancer Research
and Development Center
(301)846-1451 fax: (301)846-6619
email: jkozlovac@mail.
______________________________________________________________________________
=========================================================================
Date: Mon, 15 Mar 1999 09:46:50 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: performance evaluations
MIME-Version: 1.0
Content-Type: text/plain
As a state employee here in Colorado, we are going to a "Pay for
Performance" system.
If you have experience with this sort of system, I would appreciate feedback
(to me please) on how you have set goals, objectives, and measurement of
same. We will have a "met expectations (fully competent)", an "exceeded
expectations (peak performer)", and a "did not meet expectations (needs
improvement)" set of categories in this system.
I also supervise two employees who are responsible for lab audits, to
include performance checks on fume hoods, verification of biosafety cabinet
locations and dates of service, and probably chemical inventories in the
future. I know how many labs they need to visit and how long it takes, so I
find it easier to quantify and measure their processes and output.
Thanks in advance.
=========================================================================
Date: Mon, 15 Mar 1999 17:06:21 -0000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stuart Thompson
Subject: Re: IBC Project Reviews
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
In Manchester, England, we review every 5 years and find it a very useful
exercise. Sometimes we realize how naive we were when we made the initial
assessment.
Best wishes
Stuart
Dr Stuart Thompson
Health & Safety Services
University of Manchester
Waterloo Place
182/184 Oxford Road
Manchester M13 9PG
tel: +44 (0)161 275 5069
fax: +44 (0)161 275 6989
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Madeline J. Dalrymple
Sent: 15 March 1999 16:27
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: IBC Project Reviews
Some of our researchers gets grant renewal money to fund a recombinant DNA
research project for years after our IBC initially reviewed the project.
Yet the IBC doesn't hear about the grant renewals. The NIH guidelines
specify IBC review at initiation of a project, but nothing about reviewing
when more money is requested to continue the same research.
Do your IBCs review research for each grant request or by research projects?
Thanks
Madeline
Madeline Dalrymple
Biological Safety Officer
University of Wyoming Environmental Health and Safety Office
Laramie, Wyoming, USA
307-766-2723, fax 307-766-5678
dalrympl@uwyo.edu
=========================================================================
Date: Mon, 15 Mar 1999 10:20:51 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: Re: IBC Project Reviews
MIME-Version: 1.0
Content-Type: text/plain
I have instituted an annual review for any human gene therapy protocols--in
conjuntion with their annual review by the IRB.
I also instituted a three year review for all other protocols, as others
have said, to make certain that they are in accordance with the guidelines
and that other changes (radioactive materials, chemical hazards, etc) are
also being addressed.
=========================================================================
Date: Mon, 15 Mar 1999 13:07:00 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: autoclave records
MIME-Version: 1.0
Content-Type: text/plain
The California Health and Safety Code Section 117943 (within the Medical
Waste management Act) requires that registrants maintain individual
treatment or tracking records for three years.
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Delia Vieira [mailto:vieira@AECOM.YU.EDU]
Sent: Friday, March 12, 1999 1:05 PM
To: BIOSAFTY@mitvma.mit.edu
Subject: autoclave records
Can anyone tell me how long we have to keep autoclave records for a BSL
3
facility. Any information would be appreciated.
Delia M. Vieira-Cruz
Lab Safety Officer
Albert Einstein College of Medicine
1300 Morris Park Avenue, Forch 800
Bronx, NY 10461
(718)430-3560
vieira@aecom.yu.edu
=========================================================================
Date: Mon, 15 Mar 1999 16:13:16 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Charles Ray
Subject: Re: IBC Project Reviews
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Madeline, Auburn University requires registration renewal (or closure) at
three years. All registrations expire at the end of 6 years and must be
resubmitted.
>Some of our researchers gets grant renewal money to fund a recombinant DNA
>research project for years after our IBC initially reviewed the project.
>Yet the IBC doesn't hear about the grant renewals. The NIH guidelines
>specify IBC review at initiation of a project, but nothing about reviewing
>when more money is requested to continue the same research.
>Do your IBCs review research for each grant request or by research projects?
>
>Thanks
>Madeline
>
>
>Madeline Dalrymple
>Biological Safety Officer
>University of Wyoming Environmental Health and Safety Office
>Laramie, Wyoming, USA
>307-766-2723, fax 307-766-5678
>dalrympl@uwyo.edu
Charles H. Ray, Jr.
Director, Auburn University Office of Safety & Environmental Health
phone (334) 844-4870
fax (334) 844-4640
e-mail raychah@mail.auburn.edu
=========================================================================
Date: Tue, 16 Mar 1999 07:13:08 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: Safety Acronyms
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
>I was looking for a list or sample of some commonly used acronyms
>in safety. Examples such as OSHA-Occupational Safety and Health
>Administration, PEL-Permissible Exposure Limit and so on. This needs to be
>very basic but I want the participants to have an understanding of terms
>that they may encounter on a daily basis as they relate to safety.>
The Laboratory Safety Workshop has a very complete list of safety acronyms at
on the services page. .... Jim Kaufman
*****************************************************
James A. Kaufman, Director
The Laboratory Safety Workshop
Safety in Science Education
192 Worcester Road, Natick, MA 01760
508-647-1900 Fax: 508-647-0062 labsafe@
LSW is a national non-profit educational organization dedicated to making
health and safety an integral and important part of science education. Free
copies of our Laboratory Safety Guidelines, seminar schedule, Publications
List, AV-Lending Library List, and membership information are available on
request. The LABSAFETY-L discussion list is a public service of LSW.
******************************************************
=========================================================================
Date: Tue, 16 Mar 1999 09:49:07 -0500
Reply-To: jkeene@
Sender: A Biosafety Discussion List
From: "John H. Keene"
Subject: Re: IBC Project Reviews
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
To all of you that don't somehow review your projects each year. Remember
that the NIH guidelines are aimed primarily at lowering the "adverse" risk
of a researcher producing a new recombinant that can't be controlled. We
all know now that the likelyhood of that happening is slim and none.
However, consider that hosts and vectors may change while the basic purpose
of the research remains the same. That change in host or vector may require
a change in containment level or some other "SAFETY" related concern which
is not covered under the NIH Guidelines, but could be a real concern for
your employees and/or institution. Failure to review these changes on a
yearly basis could result in serious consequences.
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Madeline J. Dalrymple
Sent: Monday, March 15, 1999 11:27 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: IBC Project Reviews
Some of our researchers gets grant renewal money to fund a recombinant DNA
research project for years after our IBC initially reviewed the project.
Yet the IBC doesn't hear about the grant renewals. The NIH guidelines
specify IBC review at initiation of a project, but nothing about reviewing
when more money is requested to continue the same research.
Do your IBCs review research for each grant request or by research projects?
Thanks
Madeline
Madeline Dalrymple
Biological Safety Officer
University of Wyoming Environmental Health and Safety Office
Laramie, Wyoming, USA
307-766-2723, fax 307-766-5678
dalrympl@uwyo.edu
=========================================================================
Date: Tue, 16 Mar 1999 09:55:19 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: BSO -description
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Thanks for the BSO description Stuart.
=========================================================================
Date: Tue, 16 Mar 1999 10:16:29 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Madeline J. Dalrymple"
Subject: Thanks- IBC Project Reviews
MIME-version: 1.0
Content-type: text/plain; charset="iso-8859-1"
Thank you all for your comments on how often IBCs review recombinant DNA
projects. I shared the information with my IBC and it helped them come to a
decision quickly.
I appreciate your help.
Madeline
Madeline Dalrymple
Biological Safety Officer
University of Wyoming Environmental Health and Safety Office
Laramie, Wyoming, USA
307-766-2723, fax 307-766-5678
dalrympl@uwyo.edu
=========================================================================
Date: Wed, 17 Mar 1999 01:19:59 -0000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Cadaver Fluid
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Here we practice separation. Liquids will have all tissues decanted.
The effluent will be labeled and treated as chemical waste. The
tissues will be handled and treated as biohazardous materials.
There is a practical problem here created by the regulatory agencies.
Tissue and chemical would be what we call a mixed waste. EPA licensed
incinerators are not licensed to handle regulated biological
materials. Biological incinerators will not burn chemicals. They are
not authorized to do so. So we separate.
I do not know of an incinerator that is licensed to handle both. This
along with radioactive mixtures has been a vexing problem.(NRC says
let it decay while EPA says 90 days or else).
Label your biological as a biological, remove as much chemical as
possible. Label your chemical as chemical and remove all tissues.
This will avoid the misunderstanding you had.
Bob
-----Original Message-----
From: Erik Talley
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Friday, March 12, 1999 4:12 PM
Subject: Cadaver Fluid
>I need some advice about disposal of cadaver fluids from a gross
anatomy
>lab. The question has arisen after our sister campus sent some
"chemical
>waste" for incineration but labeled it as "human cadaver waste." In
>actuality, 90+% of the waste is phenol, glycerin and formaldehyde
used
>for embalming or as a wetting agent. As these liquids are collected,
>they are stored in a 55G drum for disposal.
>
>When the waste got to the incinerator it was rejected as potentially
>infectious since it had "human" in the description. After a lot of
>explanation, the disposal company now understands the solution is
>chemical waste but says since it has some human waste, they can't
accept
>it---even though it is preserved human waste therefore falling out of
>the standard.
>
>Has anyone else had problems with this or have any suggestions?
>
>Thanks,
>
>Erik
>
>
>
>____________________________________
>Erik Talley, Assistant Director
>Center for Environmental Health and Safety
>Southern Illinois University
>erik@cehs.siu.edu
>
=========================================================================
Date: Wed, 17 Mar 1999 05:33:30 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: No Subject
Mime-Version: 1.0
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For those drawing blood and performing only research procedures with that
blood, do you have a release form to be signed by the donor stating this blood
is only to be used for research or the donor is aware this blood is for
research purposes?
Is there a specific citation for this in the CFR or NIH guidelines?
Ed Krisiunas, MT(ASCP), CIC, MPH
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
=========================================================================
Date: Wed, 17 Mar 1999 08:13:31 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Heather H. Gonsoulin"
Subject: Hep B vaccine
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: quoted-printable
Can any of you OSHA gurus out there direct me to a specific standard or =
letter of interpretation that deals with Hep B vaccination in employees =
of research facilities using non-human primates. I spoke to an OSHA rep =
about this issue about a year and a half ago and was told that if you =
were not actively injecting Hep B into the animals that you were not =
required to give the Hep B series. However, I cannot find anything in =
writing about this. I really need the info and would appreciate any =
help ASAP. Thanks for your time. =20
Please respond to me personally and I will post a summary to the list.
Heather H. Gonsoulin
Occupational Health and Safety Officer
USL-NIRC
(318)482-0306 (fax) 318-373-0057
hah8377@usl.edu
=========================================================================
Date: Wed, 17 Mar 1999 08:21:01 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Michael R. Betlach"
Subject: Drawing Blood
MIME-Version: 1.0
Content-Type: text/plain
Such matters come before the Human Subjects Review Board here. Who's blood
are you taking? Researchers here will occasionally have their own blood
drawn for their research. As I understand our internal procedures, our HSB
doesn't require a release in such cases--since the researchers are aware of
the intended use. The same would not apply to blood drawn from others not
directly involved in the research, and certainly not from non-employees. A
quick look for a citation turned up the following, which provides a research
definition: 42 CFR 46.102(d)
Michael R. Betlach, Ph.D.
Biosafety Officer
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
(608) 274-1181, Ext. 1270
(608) 277-2677 FAX
mbetlach@
> -----Original Message-----
> From: Ed Krisiunas [SMTP:EKrisiunas@]
> Sent: Wednesday, March 17, 1999 4:34 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: No Subject
>
> For those drawing blood and performing only research procedures with that
> blood, do you have a release form to be signed by the donor stating this
> blood
> is only to be used for research or the donor is aware this blood is for
> research purposes?
>
> Is there a specific citation for this in the CFR or NIH guidelines?
>
> Ed Krisiunas, MT(ASCP), CIC, MPH
> INSCITE
> 115 Lyons Road
> Burlington, Connecticut
> 06013
> 860-675-1217
> 860-675-1311(fax)
=========================================================================
Date: Wed, 17 Mar 1999 09:34:45 +0500
Reply-To: speaker@ehs.psu.edu
Sender: A Biosafety Discussion List
From: Curt Speaker
Organization: UNIVERSITY SAFETY
Subject: Re: Hep B vaccine
In-Reply-To:
Heather:
You will NOT find anything in writing on this subject. OSHA has
made it very clear in numerous interpretations of the BBP standard
that it is up to each employer to determine which employees they
can "reasonably anticipate" exposures to bloodborne pathogens.
If you are not doing Hep B. work with the primates, then I would
suggest that you cannot reasonably anticipate those folks having
occupational exposure to bloodborne pathogens.
But, it is up to you to make that determination (and be able to
defend it).
Hope this helps...
Curt
Curt Speaker
Biosafety Officer
Penn State University
Environmental Health and Safety
speaker@ehs.psu.edu
^...^
(O_O)
=(Y)=
"""
=========================================================================
Date: Wed, 17 Mar 1999 09:24:26 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: Hep B vaccine
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Heather:
In addition to the other comments you have received to your inquiry, I
might add that many facilities housing NH primates like the personnel to
be vaccinated against Hepatitis B so they cannot be silent carriers of
Hepatitis B to the NH Primates. That is your decision, but I believe it
is an excellent precaution these days.
Joe Coggin
On Wed, 17 Mar 1999, Heather H. Gonsoulin wrote:
> Can any of you OSHA gurus out there direct me to a specific standard or letter of interpretation that deals with Hep B vaccination in employees of research facilities using non-human primates. I spoke to an OSHA rep about this issue about a year and a half ago and was told that if you were not actively injecting Hep B into the animals that you were not required to give the Hep B series. However, I cannot find anything in writing about this. I really need the info and would appreciate any help ASAP. Thanks for your time.
> Please respond to me personally and I will post a summary to the list.
>
> Heather H. Gonsoulin
> Occupational Health and Safety Officer
> USL-NIRC
> (318)482-0306 (fax) 318-373-0057
> hah8377@usl.edu
>
=========================================================================
Date: Wed, 17 Mar 1999 11:00:16 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Gill Norton
Organization: University of Western Ontario
Subject: Re: Drawing Blood
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Content-Transfer-Encoding: 7bit
The use of human blood for research seems to be a bit of a grey area
here. At the moment blood received for research purposes from
transfusion services does not require Human Ethics Board approval but
blood donated from individuals ( unconnected with the research
programme) specifically for a research project would and would require
the permission of the person donating it. We have a release and
indemnity form which the university signs to accept responsibility from
the transfusion service. We "discourage" researchers from donating
their own blood for their research projects because of confidentiality
issues and safety as contaminated blood accidentally transmitted back
into the person who donated it would be a hazard. However I know people
do this! I would be interested in knowing what other institutions are
doing.
Michael R. Betlach wrote:
>
> Such matters come before the Human Subjects Review Board here. Who's blood
> are you taking? Researchers here will occasionally have their own blood
> drawn for their research. As I understand our internal procedures, our HSB
> doesn't require a release in such cases--since the researchers are aware of
> the intended use. The same would not apply to blood drawn from others not
> directly involved in the research, and certainly not from non-employees. A
> quick look for a citation turned up the following, which provides a research
> definition: 42 CFR 46.102(d)
> Michael R. Betlach, Ph.D.
> Biosafety Officer
> Promega Corporation
> 2800 Woods Hollow Road
> Madison, WI 53711
> (608) 274-1181, Ext. 1270
> (608) 277-2677 FAX
> mbetlach@
>
> > -----Original Message-----
> > From: Ed Krisiunas [SMTP:EKrisiunas@]
> > Sent: Wednesday, March 17, 1999 4:34 AM
> > To: BIOSAFTY@MITVMA.MIT.EDU
> > Subject: No Subject
> >
> > For those drawing blood and performing only research procedures with that
> > blood, do you have a release form to be signed by the donor stating this
> > blood
> > is only to be used for research or the donor is aware this blood is for
> > research purposes?
> >
> > Is there a specific citation for this in the CFR or NIH guidelines?
> >
> > Ed Krisiunas, MT(ASCP), CIC, MPH
> > INSCITE
> > 115 Lyons Road
> > Burlington, Connecticut
> > 06013
> > 860-675-1217
> > 860-675-1311(fax)
--
------------------------------------------------------------------
Gillian Norton
Biosafety Officer
The University of Western Ontario
Occupational Health and Safety
Somerville House, Rm 2345
Phone: (519)661-2036
FAX: (519)661-3420
-------------------------------------------------------------------
=========================================================================
Date: Wed, 17 Mar 1999 11:10:03 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Hep B vaccine
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
In CFR 29 1910.1030, The bloodborne pathogens standard, Do a risk
dertermination to see if personnel will be exposed to bbp. If yes, the HBV
vaccination MUST be offered along with all of the other requiremnets. I do
not believe that there is a provision that allows the hbv vaccine to not be
given. Silly I know, but this is a part of a regulation:)
Bob
>Can any of you OSHA gurus out there direct me to a specific standard or
>letter of interpretation that deals with Hep B vaccination in employees of
>research facilities using non-human primates. I spoke to an OSHA rep
>about this issue about a year and a half ago and was told that if you were
>not actively injecting Hep B into the animals that you were not required
>to give the Hep B series. However, I cannot find anything in writing
>about this. I really need the info and would appreciate any help ASAP.
>Thanks for your time.
>Please respond to me personally and I will post a summary to the list.
>
>Heather H. Gonsoulin
>Occupational Health and Safety Officer
>USL-NIRC
>(318)482-0306 (fax) 318-373-0057
>hah8377@usl.edu
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Wed, 17 Mar 1999 10:31:31 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Cheri Marcham
Subject: Re: Hep B vaccine
MIME-Version: 1.0
Content-Type: text/plain
Excuse me, but the OSHA standard states that "Other Potentially Infectious
Materials" means (1) The following HUMAN body fluids: semen, vaginal
secretions, cerebrospinal fluid, synovial fluid, pleural fluid, pericardial
fluid, peritoneal fluid, amniotic fluid, saliva in dental procedures, any
body fluid that is visibly contaminated with blood, and all body fluids in
situations where it is difficult or impossible to differentiate between body
fluids;
(2) Any unfixed tissue or organ (other than intact skin) from a
HUMAN (living or dead); and
(3) HIV-containing cell or tissue cultures, organ cultures, and HIV-
or HBV-containing culture medium or other solutions; and blood, organs, or
other tissues from experimental animals infected with HIV or HBV."
(emphasis added)
The OSHA inspector was right on and the proof is in the standard in the
definitions. In no way does the standard require HBV vaccinations for
persons working with NON-HUMAN primate blood UNLESS the blood is infected
with HBV.
That doesn't mean that it's not a good idea for the other reasons cited
(protecting the non-human primates), but just that the OSHA standard is not
the reason for requiring it.
Cheri Marcham
The University of Oklahoma Health Sciences Center
> -----Original Message-----
> From: Robert N. Latsch [SMTP:rnl2@PO.CWRU.EDU]
> Sent: Wednesday, March 17, 1999 10:10 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Re: Hep B vaccine
>
> In CFR 29 1910.1030, The bloodborne pathogens standard, Do a risk
> dertermination to see if personnel will be exposed to bbp. If yes, the
> HBV
> vaccination MUST be offered along with all of the other requiremnets. I
> do
> not believe that there is a provision that allows the hbv vaccine to not
> be
> given. Silly I know, but this is a part of a regulation:)
>
> Bob
>
> >Can any of you OSHA gurus out there direct me to a specific standard or
> >letter of interpretation that deals with Hep B vaccination in employees
> of
> >research facilities using non-human primates. I spoke to an OSHA rep
> >about this issue about a year and a half ago and was told that if you
> were
> >not actively injecting Hep B into the animals that you were not required
> >to give the Hep B series. However, I cannot find anything in writing
> >about this. I really need the info and would appreciate any help ASAP.
> >Thanks for your time.
> >Please respond to me personally and I will post a summary to the list.
> >
> >Heather H. Gonsoulin
> >Occupational Health and Safety Officer
> >USL-NIRC
> >(318)482-0306 (fax) 318-373-0057
> >hah8377@usl.edu
>
>
> ________________________________________________
> __ / _______________________________________________
> _ \ / /Robert N. Latsch USSF State Referee 6 Environmental
> Safety
> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
> \ / U.S.A. RA Member Environmental
> Safety
=========================================================================
Date: Wed, 17 Mar 1999 10:49:52 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: No Subject
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Yes there should be a release form (or patient concent form), but this type
of "patient use" is classified as "exempt" from full review. This should
be handled by your institutional IRB (Internal review Board). Check with
your institutional Ofc. of Protocol Research. The applicable regulations
can be found in 21 CFR, the FDA regs, Part 54 or 56 - I think.. Go to the
web site and do a search.
Judy Pointer
Ed Krisiunas on 03/17/99 04:33:30 AM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: No Subject
For those drawing blood and performing only research procedures with that
blood, do you have a release form to be signed by the donor stating this
blood
is only to be used for research or the donor is aware this blood is for
research purposes?
Is there a specific citation for this in the CFR or NIH guidelines?
Ed Krisiunas, MT(ASCP), CIC, MPH
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
=========================================================================
Date: Wed, 17 Mar 1999 10:41:45 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Bruce Brown
Organization: The University of Texas at Houston Health Science Center
Subject: HBV titer
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
While we are on the subject of HBV - We offer the HBV vaccine to those
potentially exposed, but there is some confusion whether a HBV titer is
the responsibility of the employer or employee. I was wondering others
stance on this issue.
Bruce Brown
UT Houston Health Science Center
bbrown@admin4.hsc.uth.tmc.edu
=========================================================================
Date: Wed, 17 Mar 1999 11:35:14 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Waste Anesthetic Gas - rodents
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
We are evaluating anesthetic gas scavenging equipment for use in rodent
research operations. A supplier of inhalation anesthesia systems called
"VetEquip" has showed us some equipment that they say will do the job.
It's called an IMPAC system used for isoflurane. The rep said that many
who have bought this equipment have done monitoring on their set up - but
she did not have the data.
Do any of you use this VetEquip IMPAC system in your rodent veterinary
areas? If you do, have you monitored the attending staff for exposures?
Do you have data, we may see (confidentially)?
The equipment is expensive - we don't want to buy it (or even waste our
time testing it) if others have found it doesn't keep the gas exposures to
below the NIOSH recommended PEL - which is 2 ppm TWA.
Their literature lists many users - too numerous to list here. but some
are:
Many Pharmaceuts - Merck, Abbot, Alcon, Baxter, Chiron, Onyx, Parke-Davis,
Schering-Plough, etc.
Universities - Stanford, Tulane, U of N. Tx, U of Tenn, West Virginia,
Cornell, U of calf, Davis, Berkley, LA, San diego, San Fran,
Res Inst. - Mayo Fond. for Med. Edu., NIH, U.S. Army Med Center, Ft.
Detrick,
Thanks in advance for any input you can give me. You can reply to the list
or to me personally at
jpointer@
Judy Pointer
UT MD Anderson
Houston, Tx
713 745-1423
=========================================================================
Date: Wed, 17 Mar 1999 13:49:30 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Hep B vaccine
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Please look at paragraphs (f) Hepatitus B and post exposuer followup as
well as (f)(2) Hepatitus B vacinnation. "Hepatitus B vacinnation shall be
made available after the employee has recieved the training in paragraph
(g)(2)(vii)(I) and within 10 working days of intial assignment to all
employees who have occupational exposure unless......."
We interpret this to mean that the HBV msut be offered unless the employee
has already been vaccinated. The employee may decline the vaccination.
This does not allow the leeway to say the if employee is not being exposed
to HBV. The standard says if the employee is being exposed to BBP then hbv
vaccination must be offered. Nowhere does it qualify that hbv must be the
hazard.
The question reamins. Are the personnel being exposed to BBP?
If so offer the vaccination.
Bob
>Excuse me, but the OSHA standard states that "Other Potentially Infectious
>Materials" means (1) The following HUMAN body fluids: semen, vaginal
>secretions, cerebrospinal fluid, synovial fluid, pleural fluid, pericardial
>fluid, peritoneal fluid, amniotic fluid, saliva in dental procedures, any
>body fluid that is visibly contaminated with blood, and all body fluids in
>situations where it is difficult or impossible to differentiate between body
>fluids;
> (2) Any unfixed tissue or organ (other than intact skin) from a
>HUMAN (living or dead); and
> (3) HIV-containing cell or tissue cultures, organ cultures, and HIV-
>or HBV-containing culture medium or other solutions; and blood, organs, or
>other tissues from experimental animals infected with HIV or HBV."
>
>(emphasis added)
>
>The OSHA inspector was right on and the proof is in the standard in the
>definitions. In no way does the standard require HBV vaccinations for
>persons working with NON-HUMAN primate blood UNLESS the blood is infected
>with HBV.
>
>That doesn't mean that it's not a good idea for the other reasons cited
>(protecting the non-human primates), but just that the OSHA standard is not
>the reason for requiring it.
>
>Cheri Marcham
>The University of Oklahoma Health Sciences Center
>
>> -----Original Message-----
>> From: Robert N. Latsch [SMTP:rnl2@PO.CWRU.EDU]
>> Sent: Wednesday, March 17, 1999 10:10 AM
>> To: BIOSAFTY@MITVMA.MIT.EDU
>> Subject: Re: Hep B vaccine
>>
>> In CFR 29 1910.1030, The bloodborne pathogens standard, Do a risk
>> dertermination to see if personnel will be exposed to bbp. If yes, the
>> HBV
>> vaccination MUST be offered along with all of the other requiremnets. I
>> do
>> not believe that there is a provision that allows the hbv vaccine to not
>> be
>> given. Silly I know, but this is a part of a regulation:)
>>
>> Bob
>>
>> >Can any of you OSHA gurus out there direct me to a specific standard or
>> >letter of interpretation that deals with Hep B vaccination in employees
>> of
>> >research facilities using non-human primates. I spoke to an OSHA rep
>> >about this issue about a year and a half ago and was told that if you
>> were
>> >not actively injecting Hep B into the animals that you were not required
>> >to give the Hep B series. However, I cannot find anything in writing
>> >about this. I really need the info and would appreciate any help ASAP.
>> >Thanks for your time.
>> >Please respond to me personally and I will post a summary to the list.
>> >
>> >Heather H. Gonsoulin
>> >Occupational Health and Safety Officer
>> >USL-NIRC
>> >(318)482-0306 (fax) 318-373-0057
>> >hah8377@usl.edu
>>
>>
>> ________________________________________________
>> __ / _______________________________________________
>> _ \ / /Robert N. Latsch USSF State Referee 6 Environmental
>> Safety
>> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
>> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
>> \ / U.S.A. RA Member Environmental
>> Safety
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Wed, 17 Mar 1999 13:56:29 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: HBV titer
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
I do not remember, nor could I find any reference to hbv titers in the
standard. One would think that they would mention it if they wanted it.
The hbv vaccination must be offered. There is no requirement that the
employer check to see if the vaccination "took". There is no requirement
that titers be done to see if boosters are needed. It mentions that the
U.S. Public Health Service will decide if and when booster shots are needed.
We leave this open to the opinion and desires of the Medical Professional
and the patient. If this is sought as a result of an exposure, it is part
of our requirement to pay the medical bill. If the employee just walks in
and says that they want one. The request will be evlauated.
Bob
>While we are on the subject of HBV - We offer the HBV vaccine to those
>potentially exposed, but there is some confusion whether a HBV titer is
>the responsibility of the employer or employee. I was wondering others
>stance on this issue.
>
>Bruce Brown
>UT Houston Health Science Center
> bbrown@admin4.hsc.uth.tmc.edu
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Wed, 17 Mar 1999 14:32:40 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Re: HBV titer
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
In a message dated 3/17/99 5:58:40 AM, you wrote:
I believe there is now a recommendation for anti-body testing one to two
months after completion of the vaccination series. I'm not sure whether it
applies to all employees receiving vaccine. I heard of this recently and wrote
down the reference:
Immunizations of health-care workers: recommendations of the Advisory
Committee on Immunization Practices and HICPAC, MMWR 46:RR-18, December 26,
1997.
Ed Krisiunas, MT(ASCP), CIC, MPH
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
=========================================================================
Date: Wed, 17 Mar 1999 13:45:54 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Michael R. Betlach"
Subject: Re: HBV titer
MIME-Version: 1.0
Content-Type: text/plain
The CDC web site includes a reprint of a MMWR article MMWR 40(RR-13);1-19
Publication date: 11/22/1991, Hepatitis B Virus: A Comprehensive Strategy
for Eliminating Transmission in the United States Through Universal
Childhood Vaccination: Recommendations of the Immunization Practices
Advisory Committee (ACIP), which includes information on vaccine efficacy.
The following paragraph is excerpted from that report:
"Postvaccination testing for serologic response. Such testing is not
necessary after routine vaccination of infants, children, or adolescents.
... Postvaccination testing should also be considered for persons at
occupational risk who may have exposures from injuries with sharp
instruments, because knowledge of their antibody response will aid in
determining appropriate postexposure prophylaxis. When necessary, postvac-
cination testing should be performed from 1 to 6 months after completion of
the vaccine series. "
At our facility, we cover the costs of post-vaccination testing should an
employee request it. (The number of employees at occupational risk because
of their research activities is less than 50, to provide a context for those
with much larger programs.) At our annual BBP training, several researchers
asked about post-vaccination testing for seroconversion and also about
booster doses, emphasizing interest in and a certain anxiety about the
effectiveness of the vaccine series.
Michael R. Betlach, Ph.D.
Biosafety Officer
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
(608) 274-1181, Ext. 1270
(608) 277-2677 FAX
mbetlach@
> -----Original Message-----
> From: Bruce Brown [SMTP:bbrown@ADMIN4.HSC.UTH.TMC.EDU]
> Sent: Wednesday, March 17, 1999 12:42 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: HBV titer
>
> While we are on the subject of HBV - We offer the HBV vaccine to those
> potentially exposed, but there is some confusion whether a HBV titer is
> the responsibility of the employer or employee. I was wondering others
> stance on this issue.
>
> Bruce Brown
> UT Houston Health Science Center
> bbrown@admin4.hsc.uth.tmc.edu
=========================================================================
Date: Wed, 17 Mar 1999 15:20:00 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jose Vazquez Perez
Subject: BSL1-4 vs. P1-4
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Can anyone please explain the difference, if any, between the CDC/NIH
designation BSL 1-4 and the P 1-4 designation that is used by some
researchers (usually those trained in Europe)?
Thank you in advance,
jose vazquez
jose vazquez
Indoor Air Quality and Biosafety Coordinator
Environmental Health & Safety
(407) 823-6301
jvazquez@mail.ucf.edu
=========================================================================
Date: Wed, 17 Mar 1999 16:22:48 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jose Vazquez Perez
Subject: BSL 1-4 vs. P 1-4
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Can anyone please explain the difference, if any, between the CDC/NIH
designations BSL 1-4 and the P 1-4 used by some researchers?
Thank you,
jose vazquez
University of Central Florida
Orlando, FL
=========================================================================
Date: Thu, 18 Mar 1999 07:51:17 +1000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Sonya Watson
Subject: heat treatment of specimens
MIME-version: 1.0
Content-type: text/plain; charset="us-ascii"
Whoops! It appears the return address was incorrect when I posted this
message to the group last week. Any assistance or advice with regard to
the heat treatment of specimens and transportation would be greatly
appreciated.
Thanks,
Sonya
>Dear Biosafety members,
>
>My question to the group relates to heat treatment of specimens (in
particular blood).
>
>We are currently in the process of implementing changes to the surface
(road) transport of our specimens. The new regulations resemble the IATA
regs. As a follow on from these changes, people are now examining the
substances that they are transporting and how they can render the
substances 'non-infectious'. Two suggestions have been put forward with
regard to the heat treatment of specimens:
>
>60 deg C for 30 mins
>
>or 80 deg C for 1 hour.
>
>The purpose is to render the substance 'non-infectious'.
>
>My question is which heat treatment is recommended, and is there any
documentary evidence to back-up the destruction of blood-borne pathogens
and other potentially infectious substances via heat treatment?
>
>Thanking you in advance,
>
>Sonya
************************************
Sonya Watson
Health and Safety Adviser
School of Life Sciences
Queensland University of Technology
BRISBANE QLD AUSTRALIA
Tel: 61 7 3864 2917
Fax: 61 7 3864 1534
Email: s.watson@qut.edu.au
************************************
=========================================================================
Date: Wed, 17 Mar 1999 18:26:25 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: HBV titer
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
I have no quarrel with what is being said about what we SHOULD do. We have
a choice in these cases. I am talking about what my understanding is of
what we MUST do.
CDC, NIOSH, ACGIH and any other organization including articles in the MMW
are advisory in nature. CDC can not make us do anything. They have no
enforcement power. I can and will apply a lot of what they say anyway since
it makes good sense a further protects my employer from liability. However
it must be pointed out when push comes to shove that several practices need
not be followed because they are not in the regulations. Other practices
must be followed because they are law. When we are besieged on some of
these issues we will argue for certain items because we would like to have
them. We will give and compromise. Other items we cannot compromise on
because they are law.
Consider that violations of OSHA regulations are violations of federal law.
i.e. felonies. If I see somethng from OSHA, DOT or EPA. Especialy if it
is a regulation or an interpretation published in the Federal Register. We
had better folow it. We can go to the higher ups and say we have no
choice. The other sources of information are exactly that sources of
information. We cannot be compelled nor can we use the aurgument to the
higher ups that we are violating the law.
When we are dealing with an OSHA regulation we are being told the minimum
acceptable practice. Any other source of information can enhance what is
done. It will not protect us from ignoring these regulations.
We have such an argument here. I have stated what I believe to be the OSHA
requirements. If someone else argues for other requirements that excede
OSHA. It is icing on the cake.
Make sure you know the difference.
bob
>In a message dated 3/17/99 5:58:40 AM, you wrote:
>
>that titers be done to see if boosters are needed. It mentions that the
>U.S. Public Health Service will decide if and when booster shots are needed.
>>>
>
>I believe there is now a recommendation for anti-body testing one to two
>months after completion of the vaccination series. I'm not sure whether it
>applies to all employees receiving vaccine. I heard of this recently and wrote
>down the reference:
>
>Immunizations of health-care workers: recommendations of the Advisory
>Committee on Immunization Practices and HICPAC, MMWR 46:RR-18, December 26,
>1997.
>
>Ed Krisiunas, MT(ASCP), CIC, MPH
>INSCITE
>115 Lyons Road
>Burlington, Connecticut
>06013
>860-675-1217
>860-675-1311(fax)
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Wed, 17 Mar 1999 16:10:18 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: HBV titer
MIME-Version: 1.0
Content-Type: text/plain
At UCSF, we offer all "at-risk" employees hepatitis B vaccination, as we
must. We use the recombinant product (Recombivax) and we know it's
about 95% effective. Thus, we also know that around 1 out of 20
vaccinees will not mount a good immune response to the vaccine. We've
decided we don't want to be the test case in California courts when a
worker supposedly "protected" by an Employee Health-administered vaccine
contracts the disease. Therefore, we now routinely test all vaccinees
one month after their series and, if they don't show a clear antibody
response to the vaccination, we repeat the series. As one of those
"poor responders", I'm currently completing my second vaccine series and
will have my titer checked in a month. The post-vaccination testing is
at the expense of the employer, not the employee.
Someone mentioned boosters. It's my understanding that 60% of
individuals who were vaccinated 10-12 years ago will not show a
detectable anti-HBV titer today but that such individuals are still
protected against infection (probably statistically determined),
implying the continued presence of primed B cells that simply aren't
churning out IgGbut are ready to do so in the presence of the antigen.
For this reason, hep B boosters aren't recommended. If I'm wrong here,
or behind the times, please let me know.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Bruce Brown [mailto:bbrown@ADMIN4.HSC.UTH.TMC.EDU]
Sent: Wednesday, March 17, 1999 10:42 AM
To: BIOSAFTY@mitvma.mit.edu
Subject: HBV titer
While we are on the subject of HBV - We offer the HBV vaccine to those
potentially exposed, but there is some confusion whether a HBV titer is
the responsibility of the employer or employee. I was wondering others
stance on this issue.
Bruce Brown
UT Houston Health Science Center
bbrown@admin4.hsc.uth.tmc.edu
=========================================================================
Date: Wed, 17 Mar 1999 21:21:52 -0500
Reply-To: jkeene@
Sender: A Biosafety Discussion List
From: "John H. Keene"
Subject: Re: HBV titer
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Ed, you are correct. OSHA has left the medical stuff, including boosters
and titer determination to the CDC guidelines and if we follow those
guidelines, we will be in compliance.
Also, I've been following the questions re: Hep. B vaccination for personnel
working with primates. Hep. B is not required for this unless you are
infecting the primates with Hep. B or know that the animals are naturally
infected, (which they most likely are not). With regard to protection of
the primates, it would be easier and less costly to do Hep. B
antigen/antibody testing on the personnel than to vaccinate everybody.
Secondly, the I may be wrong, but my impression was that man and chipanzees
are the only animals susceptible to Hep B.
Finally, we have to realize that the BBP standard is a performance standard
and OSHA does not tell anyone how they must protect personnel, only that you
have to recognize when someone is at risk and provide appropriate
protection, training, medical surveillance, etc. If you are reasonably
dilligent, and can justify what you are doing, you will generally be in good
shape. What it really requires is that we THINK!
Jack Keene, Dr.P.H.
Biohaztec Associates, Inc
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Ed Krisiunas
Sent: Wednesday, March 17, 1999 2:33 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: HBV titer
In a message dated 3/17/99 5:58:40 AM, you wrote:
I believe there is now a recommendation for anti-body testing one to two
months after completion of the vaccination series. I'm not sure whether it
applies to all employees receiving vaccine. I heard of this recently and
wrote
down the reference:
Immunizations of health-care workers: recommendations of the Advisory
Committee on Immunization Practices and HICPAC, MMWR 46:RR-18, December 26,
1997.
Ed Krisiunas, MT(ASCP), CIC, MPH
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
=========================================================================
Date: Thu, 18 Mar 1999 08:25:52 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Hemphill, Mark"
Subject: Vacancy announcement
MIME-Version: 1.0
Content-Type: text/plain
The Centers for Disease Control and Prevention, Office of Health and Safety,
is currently recruiting for two interdisciplinary positions (Microbiologist,
Medical Technologist, Chemist). For those interested, the vacancy
announcement can be found on the CDC Human Resources Management Office
website at:
Mark L. Hemphill, M.S.
Office of Health and Safety
Centers for Disease Control and Prevention
1600 Clifton Rd. MS F-05
Atlanta, Georgia 30333
E-mail: MLH2@
=========================================================================
Date: Thu, 18 Mar 1999 08:15:30 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Kyle Boyett
Subject: Re: BSL1-4 vs. P1-4
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Jose, It is merely a nomenclature issue. "P" was the old designation given
to research and it stood for "personnel" protection hence...P. "BSL" (bio
safety level) is the new and designation and takes by name into account all
aspects of biosaefty. Not just personnel.
Kyle Boyett
Asst. Director of Biosafety
Occupational Health and Safety
University of Alabama at Birmingham
e-mail- kboyett@healthsafe.uab.edu
Phone- 205-934-2487
** Asking me to overlook a safety violation is like asking me to reduce the
value I place on YOUR life**
-----Original Message-----
From: Jose Vazquez Perez [mailto:jvazquez@MAIL.UCF.EDU]
Sent: Wednesday, March 17, 1999 2:20 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: BSL1-4 vs. P1-4
Can anyone please explain the difference, if any, between the CDC/NIH
designation BSL 1-4 and the P 1-4 designation that is used by some
researchers (usually those trained in Europe)?
Thank you in advance,
jose vazquez
jose vazquez
Indoor Air Quality and Biosafety Coordinator
Environmental Health & Safety
(407) 823-6301
jvazquez@mail.ucf.edu
=========================================================================
Date: Thu, 18 Mar 1999 10:06:39 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Dan Shawler
Subject: Re: HBV titer
MIME-Version: 1.0
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this format, some or all of this message may not be legible.
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Questions came up concerning the necessity and scheduling of HBV vaccine
boosters. I don't know what the literature says, but I can offer anecdotal
information. In 1992, we had two individuals who requested that we test
their anti-HBV titer. Both had been vaccinated about 1987. One individual
was negative. He received another HBV vaccine series and seroconverted to
positive. The other individual was positive. This person tests his titer
each year and was still positive as of his last test one month ago.
Dan Shawler
dshawler@
danswler@
Sidney Kimmel Cancer Center
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RE: HBV titer
Questions came up =
concerning the necessity and scheduling of HBV vaccine boosters. =
I don't know what the literature says, but I can offer anecdotal =
information. In 1992, we had two individuals who requested that =
we test their anti-HBV titer. Both had been vaccinated about =
1987. One individual was negative. He received another HBV =
vaccine series and seroconverted to positive. The other =
individual was positive. This person tests his titer each year =
and was still positive as of his last test one month ago.
Dan Shawler
dshawler@
danswler@
Sidney Kimmel =
Cancer Center
------_=_NextPart_001_01BE716A.122E207C--
=========================================================================
Date: Thu, 18 Mar 1999 13:33:11 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Diane Fleming
Subject: Re: BSL1-4 vs. P1-4
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
Correction, the "P" was for Physical containment and involved facility and
equipment as well as personal practices, it did not stand for "personnel". You
will find the "P levels" in early editions of the NIH guidelines along with
a "Biological" containment involving the use of impaired organisms. Read the
actual historical documents sometime and never stop questioning and learning.
Diane
Diane O. Fleming, Ph.D., CBSP
Biosafety Consultant
15611 Plumwood Court
Bowie, MD 20716-1434
Tel 301-249-3951
FAX 301-249-8837
email Dimerck@
=========================================================================
Date: Thu, 18 Mar 1999 16:29:40 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: Waste Anesthetic Gas - rodents
MIME-Version: 1.0
Content-Type: text/plain
Hi, Judy -
I forwarded your memo about animal anesthesia to some of our ACF folks.
Here is the relevant portion of a reply I received this afternoon. Hope
it's the kind of info you were looking for.
-- Glenn
"VetEquip is an excellent group located in Pleasanton Ca.
@1-800-772-7116.
Wendy demos these machines.
I've used the Lab Animal Anesthesia machine by Vet Equip which is less
'snazzy' than the IMPAC system your friend is testing. Although we did
not
test for exposure, I saw documentation at that time that indicated 0ppm.
The only time when you should have any reading is when there is 'pilot'
error or the machine is broken. Examples of pilot error would be
forgetting to flush the induction chamber prior to opening it, or not
having a tight fit around the patients mouth. An example of the machine
not working would be if the fittings were worn. The Animal Care
Facility
has one of these machines that is available for PI's to rent out and
try.
The basic Lab Animal Anesthesia machine system comes with 1 breathing
circuit, 1 induction chamber, and a vaporizer (Iso or Halothane). The
cost
is about $3700. You can add up to 8 additional circuits and induction
chambers but it will cost you.
If you need to do multiple rodents, then the IMPAC is what you want. It
is
the deluxe version and acts like a complete anesthesia center. It has 4
built in individual induction compartments that are switch operated. It
also has a recovery/euthanasia chamber with 02 and C02. It all exhausts
through 1 tube. This machine, if working and used correctly, also has
been tested at 0ppm.
It runs ~$7,000.
My experience is that this machine generates the least exposure to
employees, next to endotracheal intubation of the patient (as opposed to
making the patient)."
-----Original Message-----
From: Judy Pointer [mailto:jpointer@NOTES.MDACC.TMC.EDU]
Sent: Wednesday, March 17, 1999 9:35 AM
To: BIOSAFTY@mitvma.mit.edu
Subject: Waste Anesthetic Gas - rodents
We are evaluating anesthetic gas scavenging equipment for use in rodent
research operations. A supplier of inhalation anesthesia systems called
"VetEquip" has showed us some equipment that they say will do the job.
It's called an IMPAC system used for isoflurane. The rep said that many
who have bought this equipment have done monitoring on their set up -
but
she did not have the data.
Do any of you use this VetEquip IMPAC system in your rodent veterinary
areas? If you do, have you monitored the attending staff for exposures?
Do you have data, we may see (confidentially)?
The equipment is expensive - we don't want to buy it (or even waste our
time testing it) if others have found it doesn't keep the gas exposures
to
below the NIOSH recommended PEL - which is 2 ppm TWA.
Their literature lists many users - too numerous to list here. but some
are:
Many Pharmaceuts - Merck, Abbot, Alcon, Baxter, Chiron, Onyx,
Parke-Davis,
Schering-Plough, etc.
Universities - Stanford, Tulane, U of N. Tx, U of Tenn, West Virginia,
Cornell, U of calf, Davis, Berkley, LA, San diego, San Fran,
Res Inst. - Mayo Fond. for Med. Edu., NIH, U.S. Army Med Center, Ft.
Detrick,
Thanks in advance for any input you can give me. You can reply to the
list
or to me personally at
jpointer@
Judy Pointer
UT MD Anderson
Houston, Tx
713 745-1423
=========================================================================
Date: Fri, 19 Mar 1999 13:44:16 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Marsh, Robert HSD"
Subject: Hepatitis Virus
Mime-Version: 1.0
Content-Type: text/plain
How long will the Hepatitis Virons remain infective on a room
temperature surface contaminated with infected blood.
=========================================================================
Date: Fri, 19 Mar 1999 11:02:13 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: Hepatitis Virus
MIME-Version: 1.0
Content-Type: text/plain
Up to two weeks.
-----Original Message-----
From: Marsh, Robert HSD [mailto:MARSHRO@HSD.]
Sent: Friday, March 19, 1999 10:44 AM
To: BIOSAFTY@mitvma.mit.edu
Subject: Hepatitis Virus
How long will the Hepatitis Virons remain infective on a room
temperature surface contaminated with infected blood.
=========================================================================
Date: Fri, 19 Mar 1999 11:15:21 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Wolz, Sarah"
Subject: Adeno-related virus
MIME-Version: 1.0
Content-Type: text/plain
Dear List:
I'm trying to educate myself a little on the biosafety concerns and
precautions for production-scale work with adeno-related virus. I have a
few questions--and will have more, as the education process goes forth--such
as:
* recommendations for inactivating a reactor vessel prior to disposal
(of 10-100 liters growth medium)?
* would one consider vaccinating employees in the "fermentation" plant
to adenovirus?
* any reason to go beyond BSL-2 type precautions?
* would there be a susceptible sub-population to this type of virus
(immunocompromised, for example) to be concerned about?
If any of you on the list have experience working with medium to large scale
virus production lines, I'd be very interested in talking with you. Please
contact me directly at:
swolz@
(206) 559-6953
Thanks--
Sarah Wolz
EH&S Manager
PathoGenesis Corp.
=========================================================================
Date: Fri, 19 Mar 1999 15:11:53 -0500
Reply-To: jkeene@
Sender: A Biosafety Discussion List
From: "John H. Keene"
Subject: Re: Hepatitis Virus
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
According to Bond et al HBV retains viability in dried blood for at least
one week. Lancet 1981:255:1887-1891
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Marsh, Robert HSD
Sent: Friday, March 19, 1999 1:44 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Hepatitis Virus
How long will the Hepatitis Virons remain infective on a room
temperature surface contaminated with infected blood.
=========================================================================
Date: Sun, 21 Mar 1999 10:51:17 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: Re: NSTA Boston
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
For those of you attending the NSTA Meeting this week in
Boston, I would like to invite you to attend LSW's lab safety
presentations.
On Wednesday Afternoon: We'll be presenting on the OSHA
Lab Standard as part of the NSELA meeting.
On Thursday-Sunday: Bring your lab safety questions to the
Lab Safety Resource Center in the Exhibit Hall at the Carolina
Exhibit.
On Friday Evening: Join the Lab Safety Workshop and Carolina
for an "Enlightening and Entertaining Evening of Science Safety".
Food and beverages will be provided by Carolina.
On Saturday Morning: Join the Lab Safety Workshop and Carolina
for our "Safety First Seminar".
Hope to see you there, ..... Jim
=========================================================================
Date: Sun, 21 Mar 1999 18:45:13 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: Position Wanted
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
Roger Hancock is a health and safety professional with 20 years of wide-
ranging, comprehensive health and safety practice. He is certified in both
industrial hygiene and safety with coursework beyond Master's. He has broad
engineering background with strong managerial, analytical and problem-solving
capabilities.
Roger is looking for a position as a safety and health manager, safety manager
or health, safety and environmental manager for a corporation.
If you know of available positions, please contact Roger directly at
Hancock141@
Thanks for your help. ... Jim
*****************************************************
James A. Kaufman, Director
The Laboratory Safety Workshop
Safety in Science Education
192 Worcester Road, Natick, MA 01760
508-647-1900 Fax: 508-647-0062 labsafe@
LSW is a national non-profit educational organization dedicated to making
health and safety an integral and important part of science education. Free
copies of our Laboratory Safety Guidelines, seminar schedule, Publications
List, AV-Lending Library List, and membership information are available on
request. The LABSAFETY-L discussion list is a public service of LSW.
******************************************************
=========================================================================
Date: Mon, 22 Mar 1999 11:40:38 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Rachael L. Brooks"
Subject: UV sterilization
MIME-Version: 1.0
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Hello List,=20
We recently purchased a uv sterilization cabinet and it did not =
come with any instructions on what can be uv sterilized and for how =
long, etc. If I can find some sources it will be used for sterilizing =
filtration apparatus and other related items. I have not had any luck =
finding written guidelines on usage. Can anyone suggest some sources? =
Thank you in advance. Rachael=20
Rachael L. Brooks
Microbiology Lab Coordinator
Texas A&M Univ-Corpus Christi
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Hello List,
We recently =
purchased a=20
uv sterilization cabinet and it did not come with any instructions on =
what can=20
be uv sterilized and for how long, etc. If I can find some sources =
it will=20
be used for sterilizing filtration apparatus and other related =
items. I=20
have not had any luck finding written guidelines on usage. Can =
anyone=20
suggest some sources? Thank you in advance. Rachael =
Rachael L. Brooks
Microbiology =
Lab=20
Coordinator
Texas A&M =
Univ-Corpus=20
Christi
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=========================================================================
Date: Mon, 22 Mar 1999 12:58:21 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Fran Martin
Subject: Updated TSCA + SARA III CD-ROM
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Professionals responsible for health & safety may find this reference useful:
The updated U.S EPA Toxic Substance Control Act (TSCA) Chemical Inventory of
62,000 chemicals is available cross-referenced with SARA Title III RCRA
reporting requirements on CD-ROM.
It features SARA III fields integrated with TSCA information; select U.S.
Code Chapters; CORR with TSCA 4, 5, 6, 8, and 12b (export) cross-references;
EPA PMN; Canadian DSL/NDSL; European ELINCS; and Adobe(R) Acrobat(R) (PDF)
format for instant search/retrieval.
For details see
Fran Martin
FM Research & Consulting
=========================================================================
Date: Tue, 23 Mar 1999 15:58:51 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Alfred L. 'Al' Jin"
Subject: Have you seen this Warning?
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
> BioSafty Members,
Please consider this an FYI.
Al Jin, BSO, IH, CBSP,BSS(ASM),MS,CM(ACM),M(ASCP),
Lawrence Livermore National Laboratory, Livermore, CA,
Hazards Control Department, MS-289,
925 423-7385, E-mail:Jin2@
>>>> The information was sent from the Dallas Police Department to
>>>>all of the local governments in the Washington area and was
>>>>interdepartmentally dispersed. We were all asked to pass this to as
>>>>many people as possible.
>>>>
>>>>
>>>>This was from a recent CNN report:
>>>>
>>>> Subject: WARNING
>>>>
>>>> Subject: Safety Bulletin
>>>> Date; Wed,17 Feb 1999 11:23:06-0500
>>>>
>>>> For your information, a couple of weeks ago, in Dallas movie theater, a
>>>>person sat on something sharp in one of the seats. When she stood up to see
>>>>what it was, a needle was found poking through the seat with an attached
>>>>note
>>>>saying"You have been infected with H.I.V."
>>>>
>>>> The Centers for Disease Control in Atlanta reports similar events have
>>>>taken place in several other cities recently. All of the needles tested
>>>>HAVE
>>>>been positive for HIV. The CDC also reports that needles have been found in
>>>>the coin return areas of pay phones and soda machines.
>>>>
>>>> Everyone is asked to use extreme caution when confronted with these
>>>>types of situations. All public chairs should be thoroughly, but safely
>>>>inspected >>>>prior to any use. A thorough visual inspection is
>>>>considered the bare >>>>minimum.
>>>>
>>>>Furthermore, they ask that everyone notify their family members and friends
>>>>of the potential dangers, as well. ThankYou .
>>>
>>>>
=========================================================================
Date: Tue, 23 Mar 1999 17:32:21 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Tackett
Subject: Re: Have you seen this Warning?
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Did anyone think to run this by CDC, CNN or the Dallas Police
Department before sending it out to the entire list? While it may be
true, it sounds more like many of the compuer virus warnings that may
the rounds, where the actual intent is to get everyone worked up while
sending the prank warning to as many as possible
_________________________________________________________
DO YOU YAHOO!?
Get your free @ address at
=========================================================================
Date: Tue, 23 Mar 1999 20:39:41 -0800
Reply-To: jgren@
Sender: A Biosafety Discussion List
From: "Jason Gren,"
Subject: Re: Have you seen this Warning?
Content-Type: text/plain; charset=ISO-8859-1
Mime-Version: 1.0
Content-Transfer-Encoding: 7bit
Hi everyone, this is currently posted on the CDC website regarding the needlestick injuries at;
...
Are these stories true?
CDC has received inquiries about a variety of reports or warnings about used needles left by HIV-infected injection drug users in coin return slots of pay phones and movie theater seats. These reports and warnings are being circulated on the Internet and by e-mail and fax. Some reports have falsely indicated that CDC "confirmed" the presence of HIV in the needles. CDC has not tested such needles nor has CDC confirmed the presence or absence of HIV in any sample related to these rumors. The majority of these reports and warnings appear to have no foundation in fact.
CDC recently was informed of one incident in Virginia of a needle stick from a small-gauge needle (believed to be an insulin needle) in a coin return slot of a pay phone. The incident was investigated by the local police department. Several days later, after a report of this police action appeared in the local newspaper, a needle was found in a vending machine but did not cause a needle-stick injury.
Discarded needles are sometimes found in the community outside of health care settings. These needles are believed to have been discarded by persons who use insulin or are injection drug users. Occasionally the "public" and certain groups of workers (e.g., sanitation workers or housekeeping staff) may sustain needle-stick injuries involving inappropriately discarded needles. Needle-stick injuries can transfer blood and blood-borne pathogens (e.g., hepatitis B, hepatitis C, and HIV), but the risk of transmission from discarded needles is extremely low.
CDC does not recommend testing discarded needles to assess the presence or absence of infectious agents in the needles. Management of exposed persons should be done on a case-by-case evaluation of (1) the risk of a blood-borne pathogen infection in the source and (2) the nature of the injury. Anyone who is injured from a needle stick in a community setting should contact their physician or go to an emergency room as soon as possible. The injury should be reported to the local or state health departments. CDC is not aware of any cases where HIV has been transmitted by a needle-stick injury outside a health care setting.
...
Jason Gren
Canadian Food Inspection Agency
National Centre for Foreign Animal Disease
Suite T2300, 1015 Arlington Street
Winnipeg Manitoba
R3E 3M4
Canada
ph: (204)789-2073
fx: (204)789-2038
Email: jgren@em.agr.ca
Email: jgren@
Sent by Medscape Mail: Free Portable E-mail for Professionals on the Move
=========================================================================
Date: Wed, 24 Mar 1999 09:03:24 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Have you seen this Warning?
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Dear biosafty,
Please, please check before posting these things. This is a fairly recent
urban legend (check out:
). The website
is a good place to check out stories like this. I believe
YAHOO! also has a urban legend site. For computer viruses please check
out:
Thanks,
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
=========================================================================
Date: Wed, 24 Mar 1999 09:00:43 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Cheri Marcham
Subject: Re: Have you seen this Warning?
MIME-Version: 1.0
Content-Type: text/plain
Check out the CDC web site which basically says the reports aren't founded
in fact.
> -----Original Message-----
> From: Alfred L. 'Al' Jin [SMTP:e442242@POPEYE.]
> Sent: Tuesday, March 23, 1999 4:59 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Have you seen this Warning?
>
> > BioSafty Members,
>
> Please consider this an FYI.
>
> Al Jin, BSO, IH, CBSP,BSS(ASM),MS,CM(ACM),M(ASCP),
> Lawrence Livermore National Laboratory, Livermore, CA,
> Hazards Control Department, MS-289,
> 925 423-7385, E-mail:Jin2@
>
>
>
> >>>> The information was sent from the Dallas Police Department to
> >>>>all of the local governments in the Washington area and was
> >>>>interdepartmentally dispersed. We were all asked to pass this to as
> >>>>many people as possible.
> >>>>
> >>>>
> >>>>This was from a recent CNN report:
> >>>>
> >>>> Subject: WARNING
> >>>>
> >>>> Subject: Safety Bulletin
> >>>> Date; Wed,17 Feb 1999 11:23:06-0500
> >>>>
> >>>> For your information, a couple of weeks ago, in Dallas movie theater,
> a
> >>>>person sat on something sharp in one of the seats. When she stood up
> to see
> >>>>what it was, a needle was found poking through the seat with an
> attached
> >>>>note
> >>>>saying"You have been infected with H.I.V."
> >>>>
> >>>> The Centers for Disease Control in Atlanta reports similar events
> have
> >>>>taken place in several other cities recently. All of the needles
> tested
> >>>>HAVE
> >>>>been positive for HIV. The CDC also reports that needles have been
> found in
> >>>>the coin return areas of pay phones and soda machines.
> >>>>
> >>>> Everyone is asked to use extreme caution when confronted with these
> >>>>types of situations. All public chairs should be thoroughly, but
> safely
> >>>>inspected >>>>prior to any use. A thorough visual inspection is
> >>>>considered the bare >>>>minimum.
> >>>>
> >>>>Furthermore, they ask that everyone notify their family members and
> friends
> >>>>of the potential dangers, as well. ThankYou .
> >>>
> >>>>
=========================================================================
Date: Wed, 24 Mar 1999 09:19:31 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Questions about EH&S staffing
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Hi guys. I got some more questions. But b/f I barrage you with them, I'd
like to first thank everyone that responded to my Isoflurane / Anesthetic
waste gas questions, my Separation of Research and Clinical trials
questions, and my BSO description question. I'm still working on all of
these projects and will post the final outcomes - what we did - when we get
these issues resolved. FYI - We're starting to test a VetEquip unit
tomorrow. I've found more info on FDA - academic Phase I & II trial issues
- we're trying to formalize a policy. And I got back 5 BSO descriptions!
Boy what a great source of expertise! You all have given me some valuable
leads.
OK here goes on this one. My new director, Linda Lee BioSafty Members,
Please consider this an FYI.
Al Jin, BSO, IH, CBSP,BSS(ASM),MS,CM(ACM),M(ASCP),
Lawrence Livermore National Laboratory, Livermore, CA,
Hazards Control Department, MS-289,
925 423-7385, E-mail:Jin2@
>>>> The information was sent from the Dallas Police Department to
>>>>all of the local governments in the Washington area and was
>>>>interdepartmentally dispersed. We were all asked to pass this to as
>>>>many people as possible.
>>>>
>>>>
>>>>This was from a recent CNN report:
>>>>
>>>> Subject: WARNING
>>>>
>>>> Subject: Safety Bulletin
>>>> Date; Wed,17 Feb 1999 11:23:06-0500
>>>>
>>>> For your information, a couple of weeks ago, in Dallas movie theater, a
>>>>person sat on something sharp in one of the seats. When she stood up to
see
>>>>what it was, a needle was found poking through the seat with an attached
>>>>note
>>>>saying"You have been infected with H.I.V."
>>>>
>>>> The Centers for Disease Control in Atlanta reports similar events have
>>>>taken place in several other cities recently. All of the needles tested
>>>>HAVE
>>>>been positive for HIV. The CDC also reports that needles have been found
in
>>>>the coin return areas of pay phones and soda machines.
>>>>
>>>> Everyone is asked to use extreme caution when confronted with these
>>>>types of situations. All public chairs should be thoroughly, but safely
>>>>inspected >>>>prior to any use. A thorough visual inspection is
>>>>considered the bare >>>>minimum.
>>>>
>>>>Furthermore, they ask that everyone notify their family members and
friends
>>>>of the potential dangers, as well. ThankYou .
>>>
>>>>
=========================================================================
Date: Wed, 24 Mar 1999 15:33:14 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: Re: Questions about EH&S staffing
MIME-Version: 1.0
Content-Type: text/plain
Hi Judy--from lovely Denver....we are supposed to have 70 degree weather
tomorrow. My husband is ready for spring and the river rafting season.
To your questions:
> 1. Do you have an organization chart you could share? We're trying to
> find out which basic functions others cover (like fire, rad safety, waste
> pick-up, biosafety, etc.) and aprox. how many staff are allocated to each
> function.
>
We haven't got one currently that I can send on electronically. I
will attempt to put one together and forward. Have you checked out the
CSHEMA benchmarking?
Director
Rad Safety Section
2 professional staff
2 assistants
2 student workers dedicated to their program (packages,
inventory, etc)= 1 FTE
2 student workers they share with the rest of us (only they
get the majority of thier efforts)
Chem/Haz waste
1 professional staff
1 assistant
IH
1 professional staff
Compliance
2 assistants
1 professional staff (1/2) time
Biosafety
1 professional staff (1/2 time--that's me doing both)
Fire & Life Safety
2 professional staff
Building Code office
1 professional staff (expected to grow so we can build a new
campus)
Training
1 professional staff
Admin
2 FTEs
> 2. How many people are in your EH&S (or equivalent) department?
>
18 FTEs
> 3. How may people (employees &/or students) do you supply EH&S services
> too?
>
Roughly, estimate at 750 faculty & 750 staff, and 1100 students
> 4. How would you categorized your company/institute? Hospital? Research?
> University/Student Education? Pharmaceutical? Government? Private Sector?
> Other or combinations of the above?
>
University graduate schools
> I'll tally these up and let you all know, if I get enough responses. You
> can post to the list, FAX me, call, or smail mail. Thanks again.
>
> Judy Pointer, CBSP
> UT MD Anderson Cancer Institute
> Houston, Tx. 77030
> jpoint@
> Tel: 713 745-1423
> FAX: 713 745-1523
=========================================================================
Date: Thu, 25 Mar 1999 17:06:37 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: BSC (old model)
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
To member of Biosafty list
Have you heard about a brand for biosafety cabinet that is FLOW GELMAN and
GELAIRE. I think they are an Australian company. I need to contact them
for spare part urgently. I should contact them or their local
representative in Hong Kong. Please help.
Best regards.
YK Wan
The Chinese University of Hong Kong
Please reply when you receive the message. Thank you.
***** Yu Kwan WAN,
***** Safety Officer
***** The Chinese University of Hong Kong
***** Shatin, NT, Hong Kong
***** Email:
***** ulsoykwan@cuhk.edu.hk
***** ulsoykwan@
***** ulsoykwan@
***** ulsoykwan@
=========================================================================
Date: Thu, 25 Mar 1999 06:52:28 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: Questions about EH&S staffing
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Thanks Therese. That's just the kind of info we need. What does the
acronym "CSHEMA benchmarking" mean? Is this something I'm suppose to know?
- not that it shocks me that I don't!!
We are actually having 70 degree and DRY weather in Houston too. - it's
wonderful. Andy and I are going to go to northern Arizona this May or June
for hiking and rafting - up around Flagstaff. Another one of my sisters
lives there. We are still hunting for a future cooler retirement place.
We still have Wyoming and Canada to check out. So far I like SW CO best
but Andy likes New Mexico - the hot part. So I guess we're just going to
have to keep going to neat places till we can agree! See ya in the fall.
Judy
"Therese M. Stinnett" on 03/24/99 04:33:14 PM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: Re: Questions about EH&S staffing
Hi Judy--from lovely Denver....we are supposed to have 70 degree weather
tomorrow. My husband is ready for spring and the river rafting season.
To your questions:
> 1. Do you have an organization chart you could share? We're trying to
> find out which basic functions others cover (like fire, rad safety, waste
> pick-up, biosafety, etc.) and aprox. how many staff are allocated to each
> function.
>
We haven't got one currently that I can send on electronically. I
will attempt to put one together and forward. Have you checked out the
CSHEMA benchmarking?
Director
Rad Safety Section
2 professional staff
2 assistants
2 student workers dedicated to their program (packages,
inventory, etc)= 1 FTE
2 student workers they share with the rest of us (only they
get the majority of thier efforts)
Chem/Haz waste
1 professional staff
1 assistant
IH
1 professional staff
Compliance
2 assistants
1 professional staff (1/2) time
Biosafety
1 professional staff (1/2 time--that's me doing both)
Fire & Life Safety
2 professional staff
Building Code office
1 professional staff (expected to grow so we can build a
new
campus)
Training
1 professional staff
Admin
2 FTEs
> 2. How many people are in your EH&S (or equivalent) department?
>
18 FTEs
> 3. How may people (employees &/or students) do you supply EH&S services
> too?
>
Roughly, estimate at 750 faculty & 750 staff, and 1100 students
> 4. How would you categorized your company/institute? Hospital?
Research?
> University/Student Education? Pharmaceutical? Government? Private Sector?
> Other or combinations of the above?
>
University graduate schools
> I'll tally these up and let you all know, if I get enough responses. You
> can post to the list, FAX me, call, or smail mail. Thanks again.
>
> Judy Pointer, CBSP
> UT MD Anderson Cancer Institute
> Houston, Tx. 77030
> jpoint@
> Tel: 713 745-1423
> FAX: 713 745-1523
=========================================================================
Date: Thu, 25 Mar 1999 09:08:45 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Neal R. Tempel"
Subject: Re: Questions about EH&S staffing
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Why are we discussing places for retirement, vacations and the weather on this
website?
Judy Pointer wrote:
> Thanks Therese. That's just the kind of info we need. What does the
> acronym "CSHEMA benchmarking" mean? Is this something I'm suppose to know?
> - not that it shocks me that I don't!!
>
> We are actually having 70 degree and DRY weather in Houston too. - it's
> wonderful. Andy and I are going to go to northern Arizona this May or June
> for hiking and rafting - up around Flagstaff. Another one of my sisters
> lives there. We are still hunting for a future cooler retirement place.
> We still have Wyoming and Canada to check out. So far I like SW CO best
> but Andy likes New Mexico - the hot part. So I guess we're just going to
> have to keep going to neat places till we can agree! See ya in the fall.
> Judy
>
> "Therese M. Stinnett" on 03/24/99 04:33:14 PM
>
> Please respond to A Biosafety Discussion List
>
> To: BIOSAFTY@MITVMA.MIT.EDU
> cc: (bcc: Judy M. Pointer/MDACC)
> Subject: Re: Questions about EH&S staffing
>
> Hi Judy--from lovely Denver....we are supposed to have 70 degree weather
> tomorrow. My husband is ready for spring and the river rafting season.
>
> To your questions:
>
> > 1. Do you have an organization chart you could share? We're trying to
> > find out which basic functions others cover (like fire, rad safety, waste
> > pick-up, biosafety, etc.) and aprox. how many staff are allocated to each
> > function.
> >
> We haven't got one currently that I can send on electronically. I
> will attempt to put one together and forward. Have you checked out the
> CSHEMA benchmarking?
> Director
> Rad Safety Section
> 2 professional staff
> 2 assistants
> 2 student workers dedicated to their program (packages,
> inventory, etc)= 1 FTE
> 2 student workers they share with the rest of us (only they
> get the majority of thier efforts)
> Chem/Haz waste
> 1 professional staff
> 1 assistant
> IH
> 1 professional staff
> Compliance
> 2 assistants
> 1 professional staff (1/2) time
> Biosafety
> 1 professional staff (1/2 time--that's me doing both)
> Fire & Life Safety
> 2 professional staff
> Building Code office
> 1 professional staff (expected to grow so we can build a
> new
> campus)
> Training
> 1 professional staff
> Admin
> 2 FTEs
>
> > 2. How many people are in your EH&S (or equivalent) department?
> >
> 18 FTEs
>
> > 3. How may people (employees &/or students) do you supply EH&S services
> > too?
> >
> Roughly, estimate at 750 faculty & 750 staff, and 1100 students
>
> > 4. How would you categorized your company/institute? Hospital?
> Research?
> > University/Student Education? Pharmaceutical? Government? Private Sector?
> > Other or combinations of the above?
> >
> University graduate schools
>
> > I'll tally these up and let you all know, if I get enough responses. You
> > can post to the list, FAX me, call, or smail mail. Thanks again.
> >
> > Judy Pointer, CBSP
> > UT MD Anderson Cancer Institute
> > Houston, Tx. 77030
> > jpoint@
> > Tel: 713 745-1423
> > FAX: 713 745-1523
=========================================================================
Date: Thu, 25 Mar 1999 10:51:43 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: Questions about EH&S staffing
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Neal wrote -
Why are we discussing places for retirement, vacations and the weather on
this
website?
I know that one. Because Judy was still sleepy when she hit the send button
and sent it to the list instead of to Theresa directly. Sorry for bugging
you Neal - mia culpa. Judy
PS: the computer jocky that invents a way to UNSEND E-MAIL will make a
fortune.
=========================================================================
Date: Thu, 25 Mar 1999 12:18:56 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ninni Jacob
Subject: Biowaste Issues
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/enriched; charset="us-ascii"
I have a couple of questions:
1. How do you dispose of waste that is both a biohazard, and
radioactive?
For example, animal products labelled with
long-lived radioactive material, like C-14 and
H-3?
For bio-wastes containing short-lived
radioactivity, we just store for decay and then dispose of as
Biohazardous waste.
2. What do you do with AIDS research related materials (including human
blood).
Can one autoclave them and place them in regular trash (assuming they
have been disinfected and deactivated in the process)?
Thanks for any input.
Ninni Jacob, CHP
Radiation and Biological Safety Officer
Office of Risk Management
Brown University - Box 1914
164 Angell Street
Providence, RI 02912
Tel:401 863 1738 (direct)
401 863 3353
Fax:401 863 7676
email: Ninni_Jacob@brown.edu
=========================================================================
Date: Thu, 25 Mar 1999 14:34:34 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: Re: CDC BMBL 4th edition
MIME-Version: 1.0
Content-Type: text/plain
I was hoping we would see this by this time. Does anyone know when it will
be published?
Thanks.
=========================================================================
Date: Fri, 26 Mar 1999 08:42:45 +1000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Le Blanc Smith, Peter"
Subject: Re: BSC (old model)
MIME-Version: 1.0
Content-Type: text/plain
I believe Gelman are now known as (or part of) Clyde APAC Industries. Their
Web address is
. There are links to their
Australian and international distributors.
I hope you can find you spare part. Good luck.
Peter.
Peter Le Blanc Smith
Biocontainment Microbiologist
CSIRO Animal Health
Australian Animal Health Laboratory
Private Mail Bag 24
Geelong Vic 3220
Australia
Ph: (03) 5227 5451 (including voice mail)
Int: +61 3 5227 5451
Fax: (03) 5227 5555
Int: +61 3 5227 5555
E-mail address. Peter.Le.Blanc.Smith@dah.csiro.au
-----Original Message-----
From: Wan Yu Kwan [SMTP:ulsoykwan@CUHK.EDU.HK]
Sent: Friday, March 26, 1999 11:07 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: BSC (old model)
To member of Biosafty list
Have you heard about a brand for biosafety cabinet that is FLOW
GELMAN and
GELAIRE. I think they are an Australian company. I need to contact
them
for spare part urgently. I should contact them or their local
representative in Hong Kong. Please help.
Best regards.
YK Wan
The Chinese University of Hong Kong
Please reply when you receive the message. Thank you.
***** Yu Kwan WAN,
***** Safety Officer
***** The Chinese University of Hong Kong
***** Shatin, NT, Hong Kong
***** Email:
***** ulsoykwan@cuhk.edu.hk
***** ulsoykwan@
***** ulsoykwan@
***** ulsoykwan@
=========================================================================
Date: Fri, 26 Mar 1999 09:13:05 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: Re: Biowaste Issues
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
For C-14 and H-3, some of the wastes will be disposed by incineration and
dilution provided that the the radioactivity is well controlled during disposal.
YK Wan
Safety Officer
The Chinese University of Hong Kong.
At 12:18 PM 3/25/99 -0500, you wrote:
>I have a couple of questions:
>
>1. How do you dispose of waste that is both a biohazard, and radioactive?
>For example, animal products labelled with long-lived radioactive material,
like C-14 and H-3?
> For bio-wastes containing short-lived radioactivity, we just store for
decay and then dispose of as Biohazardous waste.
>
>2. What do you do with AIDS research related materials (including human blood).
>Can one autoclave them and place them in regular trash (assuming they have
been disinfected and deactivated in the process)?
>
>Thanks for any input.
>
>
>
>
>Ninni Jacob, CHP
>Radiation and Biological Safety Officer
>Office of Risk Management
>Brown University - Box 1914
>164 Angell Street
>Providence, RI 02912
>
>Tel:401 863 1738 (direct)
> 401 863 3353
>Fax:401 863 7676
>
>email: Ninni_Jacob@brown.edu
>
Please reply when you receive the message. Thank you.
***** Yu Kwan WAN,
***** Safety Officer
***** The Chinese University of Hong Kong
***** Shatin, NT, Hong Kong
***** Email:
***** ulsoykwan@cuhk.edu.hk
***** ulsoykwan@
***** ulsoykwan@
***** ulsoykwan@
=========================================================================
Date: Fri, 26 Mar 1999 11:14:02 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ninni Jacob
Subject: Re: Biowaste Issues
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
I guess I should have been more specific.
What do you do with sharps (syringes and needles containing animal blood)
that were used to inject animals with radioactivity (namely, H3 and C14),
and later draw the blood for counting.
Thanks.
Ninni Jacob, CHP
Radiation and Biological Safety Officer
Office of Risk Management
Brown University - Box 1914
164 Angell Street
Providence, RI 02912
Tel:401 863 1738 (direct)
401 863 3353
Fax:401 863 7676
email: Ninni_Jacob@brown.edu
=========================================================================
Date: Fri, 26 Mar 1999 15:42:49 -0500
Reply-To: rubockpa@UMDNJ.EDU
Sender: A Biosafety Discussion List
From: Paul Rubock
Organization: eohss-umdnj
Subject: Safety Features-Tissue Culture for Transplant
MIME-Version: 1.0
Content-Type: multipart/alternative;
boundary="------------5550D361D773B72D30D3F4BD"
--------------5550D361D773B72D30D3F4BD
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
The following question was directed to my by a researcher at my
institution:
"What regulations are present for cell culture facilities to harvest and
possibly grow tissue/cells that will be used for patients?"
"We will be isolating epithelial cells or retinal sheets from human eyes
and want to do it in a manner that would be the safest for patients.
I'm sure there are regulations in place that assure the safety issue."
Outside of using BSL-2 practices (particularly manipulating cell
cultures only in a BSC) to protect the culturists, the question is how
to ensure that trasnsplanted materials are sufficiently "safe" for
recipients. Would an engineering control such as HEPA-filtered supply
be necessary...Any suggestions for QC tests on material prior to
injection?
In some cases, the transplants will be autologous, in others, the tissue
will be obtained from cadavers-what type of health info. should be
available, particularly in the latter case?
Seems to me like the minimum PPE would be head covering, surg. mask,
disposable scrubs, gloves, shoe covers-any thing else? Should gloves be
sterile or just regular exam type?
Thanks for any help.
Paul Rubock
UMDNJ
--------------5550D361D773B72D30D3F4BD
Content-Type: text/html; charset=us-ascii
Content-Transfer-Encoding: 7bit
The following question was directed to my by a researcher at my institution:
"What regulations are present for cell culture facilities
to harvest and possibly grow tissue/cells that will be used for patients?"
"We will be isolating epithelial cells or retinal sheets
from human eyes and want to do it in a manner that would be the safest
for patients. I'm sure there are regulations in place that assure
the safety issue."
Outside of using BSL-2 practices (particularly manipulating cell cultures
only in a BSC) to protect the culturists, the question is how to ensure
that trasnsplanted materials are sufficiently "safe" for recipients.
Would an engineering control such as HEPA-filtered supply be necessary...Any
suggestions for QC tests on material prior to injection?
In some cases, the transplants will be autologous, in others, the tissue
will be obtained from cadavers-what type of health info. should be available,
particularly in the latter case?
Seems to me like the minimum PPE would be head covering, surg. mask,
disposable scrubs, gloves, shoe covers-any thing else? Should gloves
be sterile or just regular exam type?
Thanks for any help.
Paul Rubock
UMDNJ
--------------5550D361D773B72D30D3F4BD--
=========================================================================
Date: Sat, 27 Mar 1999 21:49:36 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Animal Bite Protocols
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
I had sent out a request for an animal bite protocol. I received only one
response. However, I also received three inquiries as to whether I ahd heard
anyhting. So I went looking on my own.
For an animal bite protocol, go to the following web sites:
(University of Florida)
and
(University of Medicine and Dentistry
of New Jersey)
Regards,
Ed Krisiunas, MT(ASCP), CIC, MPH
INSCITE
>115 Lyons Road
>Burlington, Connecticut
>06013
>860-675-1217
>860-675-1311(fax)
=========================================================================
Date: Mon, 29 Mar 1999 14:07:51 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Taylor, David G. PHD"
Subject: Re: CDC BMBL 4th edition
MIME-Version: 1.0
Content-Type: text/plain
We expect it to go to the publisher in early April. We estimate that the
book may be available in late May or early June.
Dave Taylor, PhD, CBSP
Deputy Director
Office of Health and Safety, CDC
> -----Original Message-----
> From: Therese M. Stinnett [SMTP:Therese.Stinnett@UCHSC.EDU]
> Sent: Thursday, March 25, 1999 4:35 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Re: CDC BMBL 4th edition
>
> I was hoping we would see this by this time. Does anyone know when it
> will
> be published?
>
> Thanks.
=========================================================================
Date: Wed, 31 Mar 1999 16:28:32 +0100
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Debra Sharpe
Subject: Position- Fire Protection Engineer
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Fire Protection Engineer
Auburn University
Responsible to the Associate Director of Safety and Environmental Health
for developing, organizing and managing various fire protection programs to
insure University compliance with all applicable national and state fire
safety requirements. This includes review of construction plans for fire
code compliance and equivalent facility design standards, fire protection
acceptance tests, facility inspections, fire drills, fire safety training,
fire investigation, participation in various committees, maintenance of
fire protection systems and fire extinguisher contract. This person will
act as a liaison between the University and Auburn City Fire Department.
Good communication and negotiation skills are a must. This person will
work closely with the Facilities Division Plant Services group, the
University Architect's Office, the Office of Risk Management and the Auburn
Fire Department.
No direct reports but this position may supervise students or other staff
assigned to particular projects.
Requirements:
Education: BS in Fire Protection Engineering , the Fire Sciences or other
engineering degree, from an accredited four-year institution.
Experience: Desire two or more years experience as a Fire Protection
Engineer, extensive computer, written and interpersonal skills are required.
Salary Salary will be based on training and pertinent experience.
Starting salary for this position will be $25,885 - $39,935. Auburn
University provides an attractive benefits package.
Submit a letter of interest, resume' and the names of three references to:
Auburn University Human Resources
Employment Section
Langdon Hall
Auburn University, AL 36849-5126
For further information, please contact Ms. Debra Sharpe, Associate
Director, at (334) 844-4870 or sharpdc@mail.auburn.edu. Review of
Applicants will begin on May 5, 1999. Women and minorities are encouraged
to apply. Auburn University is an Affirmative Action/Equal Employment
Opportunity employer.
D. C. Sharpe, CCHO
Associate Director
Safety and Environmental Health
313 Leach Science Bldg
Auburn University, 36849
Ph (334) 844-4870
fax 4640
=========================================================================
Date: Wed, 31 Mar 1999 22:13:11 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Larry L.Triplett"
Subject: Re: Position- Fire Protection Engineer
In-Reply-To:
MIME-version: 1.0
Content-type: text/plain; charset="us-ascii"
FYI. Hope your trip to Fla was as good as mine. LLT
At 04:28 PM 3/31/99 +0100, you wrote:
>Fire Protection Engineer
>Auburn University
>
>Responsible to the Associate Director of Safety and Environmental Health
>for developing, organizing and managing various fire protection programs to
>insure University compliance with all applicable national and state fire
>safety requirements. This includes review of construction plans for fire
>code compliance and equivalent facility design standards, fire protection
>acceptance tests, facility inspections, fire drills, fire safety training,
>fire investigation, participation in various committees, maintenance of
>fire protection systems and fire extinguisher contract. This person will
>act as a liaison between the University and Auburn City Fire Department.
>
>Good communication and negotiation skills are a must. This person will
>work closely with the Facilities Division Plant Services group, the
>University Architect's Office, the Office of Risk Management and the Auburn
>Fire Department.
>
>No direct reports but this position may supervise students or other staff
>assigned to particular projects.
>
>Requirements:
>
>Education: BS in Fire Protection Engineering , the Fire Sciences or other
>engineering degree, from an accredited four-year institution.
>
>
>Experience: Desire two or more years experience as a Fire Protection
>Engineer, extensive computer, written and interpersonal skills are required.
>
>Salary Salary will be based on training and pertinent experience.
>Starting salary for this position will be $25,885 - $39,935. Auburn
>University provides an attractive benefits package.
>
>Submit a letter of interest, resume' and the names of three references to:
> Auburn University Human Resources
> Employment Section
> Langdon Hall
> Auburn University, AL 36849-5126
>
>For further information, please contact Ms. Debra Sharpe, Associate
>Director, at (334) 844-4870 or sharpdc@mail.auburn.edu. Review of
>Applicants will begin on May 5, 1999. Women and minorities are encouraged
>to apply. Auburn University is an Affirmative Action/Equal Employment
>Opportunity employer.
>
>
>
>D. C. Sharpe, CCHO
>Associate Director
>Safety and Environmental Health
>313 Leach Science Bldg
>Auburn University, 36849
>Ph (334) 844-4870
>fax 4640
>
=========================================================================
Date: Thu, 1 Apr 1999 09:20:06 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Instructions for signing on to the list
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7bit
Where can I find instructions for signing on to the list that I can forward to
a colleague?
Ed Krisiunas
Dirertor
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
=========================================================================
Date: Thu, 1 Apr 1999 10:24:35 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Re: Instructions for signing on to the list
MIME-Version: 1.0
Content-Type: text/plain
On the ABSA web page.
click on mailing lists on the left side.
Bliss M. Schlank
Biosafety Specialist
Zeneca Pharmaceuticals
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185
Fax: 302.886.2909
bliss.schlank@phwilm.
> ----------
> From: Ed Krisiunas[SMTP:EKrisiunas@]
> Sent: Thursday, April 01, 1999 9:20 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Instructions for signing on to the list
>
> Where can I find instructions for signing on to the list that I can
> forward to
> a colleague?
>
> Ed Krisiunas
> Dirertor
> INSCITE
> 115 Lyons Road
> Burlington, Connecticut
> 06013
> 860-675-1217
> 860-675-1311(fax)
>
=========================================================================
Date: Sun, 4 Apr 1999 14:22:19 EDT
Reply-To: Labsafe@
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: New Lab Safety Poster
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
The Laboratory Safety Workshop has a new Guidelines
Poster. LSW's "Laboratory Safety Guidelines: 40 Suggestions
for a Safer Lab" are now available in an attractive, colorful, and fun
24" x 36" poster.
Thanks to Carolina Biological Supply Company for the graphics,
design, and printing.
*****************************************************
James A. Kaufman, Director
The Laboratory Safety Workshop
Safety in Science Education
192 Worcester Road, Natick, MA 01760
508-647-1900 Fax: 508-647-0062 labsafe@
LSW is a national non-profit educational organization dedicated to making
health and safety an integral and important part of science education. Free
copies of our Laboratory Safety Guidelines, seminar schedule, Publications
List, AV-Lending Library List, and membership information are available on
request. The LABSAFETY-L discussion list is a public service of LSW.
******************************************************
=========================================================================
Date: Mon, 5 Apr 1999 08:43:40 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Instructions for signing on to the list
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
At 09:20 AM 4/1/99 EST, you wrote:
>Where can I find instructions for signing on to the list that I can
forward to
>a colleague?
>
ABSA web site () OR:
To subscribe:
Send an email to listserv@mitvma.mit.edu
In the body of the mail type:
Sub biosafty Yourfirstname Yourlastname
DO NOT include a signature file.
>
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Mon, 5 Apr 1999 15:28:11 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Karen Hill
Subject: in need of a BL-3 facility offering training
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Biosafety subscribers
I am currently at a laboratory that is hoping to operate a BL-3 facility in
the near future. Two technicians and our facility manager would like to be
trained at an "experienced" BL-3 facility --one that has handled different
types of BL-3 bacteria-the culture of anaerobic and aerobic bacteria and
one that has been in operation for several years. If you know of such a
facility that would train these personnel in the next several months please
email me at hill@telomere.
=========================================================================
Date: Mon, 5 Apr 1999 15:54:59 -0600
Reply-To: terrie@cc.usu.edu
Sender: A Biosafety Discussion List
From: Terrie Wierenga
Organization: USDA-ARS PPRL
Subject: tire buffings for bedding
MIME-version: 1.0
Content-type: text/plain; charset=us-ascii
Content-transfer-encoding: 7bit
Our facility animal care supervisor would like to use tire buffings
(from a local retread shop) as bedding for our large animals (cattle,
goats, and sheep). The buffings contain no metal shavings; they're just
the rubber from the tires brought in for retreading. Has (or is) anyone
using tire buffings for animal bedding?
Some of the concerns we have include:
1. possible health impacts on the animals, i.e., does standing,
sitting, or nibbling on 4+ inches of rubber shavings expose the animals
to anything of concern?
2. would the rubber present additional health hazards to the people
working the animals?
3. environmental impacts: currently the buffings are being taken to
the local landfill, so there shouldn't be a problem continuing that
practice. However, I'm pretty sure folks wouldn't want the manure we
scrape from our lots to have pieces of rubber in it when they come in
for this "natural" fertilizer. And so we lose our current practice of
'recycling' the manure on various garden plots and fields in the valley.
I'd appreciate any thoughts you have on this subject.
Terrie
--
****
Terrie Wierenga, CDSO, LRPO
USDA-ARS Poisonous Plant Research Lab
1150 East 1400 North
Logan, UT 84341
v: 435-752-2941
f: 435-753-5681
e: terrie@cc.usu.edu
=========================================================================
Date: Tue, 6 Apr 1999 10:52:33 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: FW: Biosafety Lecture Materials
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
> Dear Biosafety Subscribers:
>
> Please respond directly to David Wolff at: dwolff@iastate.edu
> (do not respond to the BIOSAFTY list)
>
> A co-worker would like to know if anyone on the list would be willing to
> share a PowerPoint presentation or other materials suitable for lecture
> use on occupational biosafety. The lecture is part of a survey of
> industrial hygiene. Mr. Wolff is interested in recombinant DNA and
> infectious materials safety in the workplace - laboratory or other.
>
> References (journal or newspaper) that provide a "big picture" of current
> trends and thinking biosafety would be greatly appreciated as well.
>
>
Julie A. Johnson, Ph.D.
Biosafety Officer
Iowa State University
Environmental Health & Safety
118 Agronomy Lab
phone: 515-294-7657
fax: 515-294-9357
=========================================================================
Date: Tue, 6 Apr 1999 11:04:55 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: FW: Biosafety Lecture Materials
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
My apologies if this got sent twice - I received a message that it was
returned undeliverable.
----------------------------------------------------------------------------
-------------------------------------------------------
> Dear Biosafety Subscribers:
>
> Please respond directly to David Wolff at: dwolff@iastate.edu
> (do not respond to the BIOSAFTY list)
>
> A co-worker would like to know if anyone on the list would be willing to
> share a PowerPoint presentation or other materials suitable for lecture
> use on occupational biosafety. The lecture is part of a survey of
> industrial hygiene. Mr. Wolff is interested in recombinant DNA and
> infectious materials safety in the workplace - laboratory or other.
>
> References (journal or newspaper) that provide a "big picture" of current
> trends and thinking biosafety would be greatly appreciated as well.
>
Julie A. Johnson, Ph.D.
Biosafety Officer
Iowa State University
Environmental Health & Safety
118 Agronomy Lab
phone: 515-294-7657
fax: 515-294-9357
=========================================================================
Date: Tue, 6 Apr 1999 11:17:05 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: FW: Biosafety Lecture Materials
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
> Dear Biosafety Subscribers:
>
> Please respond directly to David Wolff at: dwolff@iastate.edu
> (do not respond to the BIOSAFTY list)
>
> A co-worker would like to know if anyone on the list would be willing to
> share a PowerPoint presentation or other materials suitable for lecture
> use on occupational biosafety. The lecture is part of a survey of
> industrial hygiene. Mr. Wolff is interested in recombinant DNA and
> infectious materials safety in the workplace - laboratory or other.
>
> References (journal or newspaper) that provide a "big picture" of current
> trends and thinking biosafety would be greatly appreciated as well.
>
>
>
Julie A. Johnson, Ph.D.
Biosafety Officer
Iowa State University
Environmental Health & Safety
118 Agronomy Lab
phone: 515-294-7657
fax: 515-294-9357
=========================================================================
Date: Tue, 6 Apr 1999 13:03:39 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Kevin Roberts
Organization: The University of Texas at Houston Health Science Center
Subject: Fungicidal Protective Coatings
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
A colleague of mine wanted to pose a question to the Biosafety List but
could not because he does not subscribe to the list. The message he
attempted to send is below. Please reply to me directly and then I will
post the responses at a later date. Thank you.
Kevin Roberts
The University of Texas Houston Health Science Center
---------------------------------
I would like some views or experiences related to applying fungicidal
protective coatings, i.e. Foster 40-20 or Foster 40-80 on the interior
of ducts of HVAC systems. Does it work well? Is cleaning the ducts not
enough? How about odor and irritation complaints from people working in
the building after the coatings are applied?
Thanks,
Joe Parham
University of Texas Houston Health Science Center
=========================================================================
Date: Tue, 6 Apr 1999 15:02:27 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Carol Showalter
Subject: Decontamination Validation Testing
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Recent discussions with colleagues have raised some issues about which I
would appreciate your input.
1. For those programs that utilize a spore strip in the validation of
biocabinet decontamination, broth tubes (containing the strip) are
incubated for what length of time for the test to be considered "OK" and
the decon valid?
2. Do you use this process primarily as a QC check or is work in/on the
cabinet dependent upon the stip remaining negative after a specific # of days?
Thank you,
Carol
Carol Showalter
Biosafety Professional
Health Protection Office
The University of Iowa
E-Mail:carol-showalter@uiowa.edu
Tel:319-335-9553
Fax:319-335-7564
=========================================================================
Date: Tue, 6 Apr 1999 17:11:09 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Don Callihan
Subject: Re: in need of a BL-3 facility offering training
Mime-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Don Callihan@BDX
04/06/99 05:11 PM
Name and phone number of whoever posted this would be helpful.
Don Callihan
Biosafety Officer
Becton Dickinson Biosciences
410.773.6684
hill@TELOMERE. on 04/05/99 05:28:11 PM
Please respond to BIOSAFTY@MITVMA.MIT.EDU
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Don Callihan/USAMD/BDX)
Subject: in need of a BL-3 facility offering training
Biosafety subscribers
I am currently at a laboratory that is hoping to operate a BL-3 facility in
the near future. Two technicians and our facility manager would like to be
trained at an "experienced" BL-3 facility --one that has handled different
types of BL-3 bacteria-the culture of anaerobic and aerobic bacteria and
one that has been in operation for several years. If you know of such a
facility that would train these personnel in the next several months please
email me at hill@telomere.
=========================================================================
Date: Tue, 6 Apr 1999 17:11:24 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Sandra Fry
Subject: photos of CL/BSL 4's...
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; CHARSET=ISO-8859-1
I am doing a display on Biocontainment for a laboratory open house and I
need photos of BSL 4 facilities (staff working, PPE, physical
characteristics, etc). Does anyone have a resource for this? I can't find
any images on the internet .......
Sandra C. Fry
Biosafety Officer
BC Centre for Disease Control,
655 W. 12th Avenue
Vancouver, BC V5Z 4R4
phone: (604) 660 6167
pager: (604) 893 5737
fax: (604) 660 6073
=========================================================================
Date: Wed, 7 Apr 1999 09:45:39 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: Re: Decontamination Validation Testing
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Dear Caro
We use the spore strip for validation of decomtamination. We usually
incubate 24 hour although the manufacture recommend 7 days. The maintenance
and certification will be performed if all of the 3 strips is negative.
YK Wan
Safety Officer
The Chinese University of Hong Kong
At 03:02 PM 4/6/99 -0500, you wrote:
>Recent discussions with colleagues have raised some issues about which I
>would appreciate your input.
>
>1. For those programs that utilize a spore strip in the validation of
>biocabinet decontamination, broth tubes (containing the strip) are
>incubated for what length of time for the test to be considered "OK" and
>the decon valid?
>
>2. Do you use this process primarily as a QC check or is work in/on the
>cabinet dependent upon the stip remaining negative after a specific # of days?
>
>Thank you,
>Carol
>Carol Showalter
>Biosafety Professional
>Health Protection Office
>The University of Iowa
>E-Mail:carol-showalter@uiowa.edu
>Tel:319-335-9553
>Fax:319-335-7564
>
>
Please reply when you receive the message. Thank you.
***** Yu Kwan WAN,
***** Safety Officer
***** The Chinese University of Hong Kong
***** Shatin, NT, Hong Kong
***** Email:
***** ulsoykwan@cuhk.edu.hk
***** ulsoykwan@
***** ulsoykwan@
***** ulsoykwan@
=========================================================================
Date: Tue, 6 Apr 1999 23:25:53 -0400
Reply-To: grobertson@
Sender: A Biosafety Discussion List
From: "George A. Robertson"
Subject: Re: photos of CL/BSL 4's...
MIME-Version: 1.0
Content-Type: multipart/alternative;
boundary="----=_NextPart_000_01BE8084.D0593C20"
Content-Transfer-Encoding: 7bit
This is a multi-part message in MIME format.
------=_NextPart_000_01BE8084.D0593C20
Content-Type: text/plain; charset=ISO-8859-1
Content-Transfer-Encoding: 7bit
Check out the CDC website. When you download pictures from the web, a copy
is saved in your "temporary internet file" (.jpg or .gif). You can save
that image to another folder. Then insert the image into a power point or
word file. Also, there is a complete biosafety slide set on that site, as
well.
Good luck,
George Robertson
George A. Robertson, PhD
BioReliance Corporation
9900 Blackwell Rd
Rockville, MD 20850
301.610.2834
301.738.1036 (FAX)
----------
> From: Sandra Fry
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: photos of CL/BSL 4's...
> Date: Tuesday, April 06, 1999 9:11 PM
>
> I am doing a display on Biocontainment for a laboratory open house and I
> need photos of BSL 4 facilities (staff working, PPE, physical
> characteristics, etc). Does anyone have a resource for this? I can't find
> any images on the internet .......
>
>
> Sandra C. Fry
> Biosafety Officer
> BC Centre for Disease Control,
> 655 W. 12th Avenue
> Vancouver, BC V5Z 4R4
> phone: (604) 660 6167
> pager: (604) 893 5737
> fax: (604) 660 6073
------=_NextPart_000_01BE8084.D0593C20
Content-Type: text/html; charset=ISO-8859-1
Content-Transfer-Encoding: quoted-printable
Check out the CDC website. When =
you download pictures from the web, a copy is saved in your =
"temporary internet file" (.jpg or .gif). You can =
save that image to another folder. Then insert the image =
into a power point or word file. Also, there is a complete =
biosafety slide set on that site, as well.Good =
luck,George Robertson =
<><><><><><><><=
;><><><>George A. Robertson, =
PhDBioReliance Corporation9900 Blackwell RdRockville, MD =
20850301.610.2834301.738.1036 (FAX)<; From: Sandra Fry =
<sandra.fry@BCCDC.HNET.BC.CA>> To: BIOSAFTY@MITVMA.MIT.EDU> Subject: photos of CL/BSL 4's...> =
Date: Tuesday, April 06, 1999 9:11 PM> > I am doing a =
display on Biocontainment for a laboratory open house and I> need =
photos of BSL 4 facilities (staff working, PPE, physical> =
characteristics, etc). Does anyone have a resource for this? I can't =
find> any images on the internet .......> > =
> Sandra C. Fry> Biosafety Officer> BC Centre for =
Disease Control,> 655 W. 12th Avenue> Vancouver, BC V5Z =
4R4> phone: (604) 660 6167> pager: (604) 893 5737> =
fax: (604) 660 6073
------=_NextPart_000_01BE8084.D0593C20--
=========================================================================
Date: Wed, 7 Apr 1999 08:44:01 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Decontamination Validation Testing
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
>1. For those programs that utilize a spore strip in the validation of
>biocabinet decontamination, broth tubes (containing the strip) are
>incubated for what length of time for the test to be considered "OK" and
>the decon valid?
In general, the strips will go positive within a week. I have had some
experiences where it took 8 days, so we normally keep them 2 weeks.
>
>2. Do you use this process primarily as a QC check or is work in/on the
>cabinet dependent upon the stip remaining negative after a specific # of
days?
Primarily a QC check.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Wed, 7 Apr 1999 09:36:08 -0400
Reply-To: gilpin@welch.jhu.edu
Sender: A Biosafety Discussion List
From: "Richard Gilpin, Ph.D., RBP, CBSP"
Organization: Johns Hopkins Institutions
Subject: Johns Hopkins University Biosafety Course Announcement
MIME-Version: 1.0
Content-Type: multipart/mixed; boundary="------------2B43EA7B8F6D30E5F1E4AF82"
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The Annual (for twenty years) Johns Hopkins University
Biosafety Course entitled
"Control of Biohazards in the Research Laboratory"
will be held in Baltimore, Maryland at the Omni Inner Harbor
Hotel.
The dates are from Monday June 14, 1999 through Friday June
18, 1999.
The course announcement is attached in MS Word 97.
Please email me if you experience problems opening the
attachment.
Thanks,
Richard
--
Richard W. Gilpin, Ph.D., RBP, CBSP
Biosafety Officer, Biosafety Division
Assistant Professor of Medicine (SOM)
Assistant Professor of Environmental Health Sciences (SHPH)
Department of Health, Safety & Environment
Johns Hopkins Institutions
2024 East Monument Street, Room 2-700
Baltimore MD 21205-2223
Phone (410) 955-5918
Fax (410) 955-5929
Internet Email: gilpin@welch.jhu.edu
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--------------2B43EA7B8F6D30E5F1E4AF82--
=========================================================================
Date: Wed, 7 Apr 1999 09:00:03 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Alfred L. 'Al' Jin"
Subject: Re: Decontamination Validation Testing
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Carol,
Spore strips should be used in accordance to manufacturer instructions.
Usually it does not exceed 7 days. Specific temperatures will depend on
sterilization method chosen. Steam sterilization and Chemical vapor methods
(VHP) require incubation of Bacillus stearothermophilus spore strips at 56
C. Dry heat and EtO requires incubation of Bacillus subtilis var. niger
spore strips at 37 C. Radiation sterilization methods will require
incubation of Bacillus pumilus spore strips at 37 C (Unsure of temp????)
Each test strip culture should be observed daily for 7 days. Any positive
strips usually is indicated by a specific color change. All positives
should routine be gram stain to rule out possible contamination from poor
sterile technique. Gram stains yielding a bacillus sp. would be
confirmation of a positive QC test. Other organisms microscopically
observed would indicate a false positive and should be declared as an
invalid test. The load should be retested.
In closing, I hope this information is helpful.
Al Jin, BSO, IH, CBSP, BSM(ASM), MS, CM(ACM), M(ASCP),
Lawrence Livermore National Laboratory, Livermore, CA,
Hazards Control Department, MS-289, 925 423-7385,
E-mail:Jin2@
>Recent discussions with colleagues have raised some issues about which I
>would appreciate your input.
>
>1. For those programs that utilize a spore strip in the validation of
>biocabinet decontamination, broth tubes (containing the strip) are
>incubated for what length of time for the test to be considered "OK" and
>the decon valid?
>
>2. Do you use this process primarily as a QC check or is work in/on the
>cabinet dependent upon the stip remaining negative after a specific # of days?
>
>Thank you,
>Carol
>Carol Showalter
>Biosafety Professional
>Health Protection Office
>The University of Iowa
>E-Mail:carol-showalter@uiowa.edu
>Tel:319-335-9553
>Fax:319-335-7564
=========================================================================
Date: Thu, 8 Apr 1999 12:30:55 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Barbara Ernisse
Subject: Lab Cleaning Procedures Needed
In-Reply-To:
MIME-version: 1.0
Content-type: TEXT/PLAIN; CHARSET=US-ASCII
Content-transfer-encoding: 7BIT
Good Morning.
Can someone help with this? Our clinical genetics lab is moving to space that
was previously used for bacteriology and virology pcr, DNA/RNA gels and
associated photography. They are worried about cross-contamination of their DNA
with material from the virology and bacteriology procedures. No cultures were
held in the rooms, purified DNA was brought up from the culture facilities for
amplification and subsequent manipulation.
This is not a disinfection problem but the clinical geneticists want a
decontamination to remove any residual DNA from the surfaces. Anyone have any
experience in this? I would greatly appreciate assistance in finding
procedures, materials or contractors in the Boston area. Thank you
Barb Ernisse
Children's Hospital
=========================================================================
Date: Thu, 8 Apr 1999 13:34:43 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Robin Newberry
Subject: Re: Lab Cleaning Procedures Needed
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Barb,
On the room decons I've worked in the past, we used a variation on the BSC
decon procedure; i.e. we sealed the room completely air tight, and
generated a formaldehyde gas in the room to about 16,000 ppm and left it
over night.
Of course, if the room is not completely tight you'll probably have to
evacuate the building; this includes gasketed dampers in the HVAC ducting.
And you'll need some way to vent it once you're through. Ideally you'd do
this remotely, as you don't want to have to suit up and put on SCBA to
enter the room if at all possible.
Robin
W. Robert Newberry, IV CIH, CHMM
Director, Environmental Health and Safety
Clemson University
wnewber@clemson.edu ehs@clemson.edu
=========================================================================
Date: Thu, 8 Apr 1999 13:09:38 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Cipriano,Mary"
Subject: Re: Lab Cleaning Procedures Needed
Mime-Version: 1.0
Content-Type: text/plain; charset=iso-8859-1
Content-Transfer-Encoding: quoted-printable
Content-Transfer-Encoding: quoted-printable
We use a detergent followed by a 20% bleach solution to break down the =
DNA on
contaminated surfaces. We found that a 10% bleach solution was not adeq=
uate.
Mary Cipriano
Abbott Labs
=
=========================================================================
Date: Thu, 8 Apr 1999 14:44:56 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Francis Churchill
Subject: Re: Lab Cleaning Procedures Needed
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
We had a similar issue when a mamalian tissue culture lab was moving into a
lab which had been used for mycology; the new researcher was concerned and
wanted to have the room decontaminated. As this was in our newest
building, which had been designed for laboratories; and has yet to be
redesigned enough time to totally mess up the air handling system; and is
100% fresh air supply / 100% exhaust, we gassed the room with formaldehyde.
We used B&V Testing from Waltham, Mass (781)647-3770. We did this after
hours and gave a couple of days notice for people to clear the areas around
this lab.
Another time we used ENV Services 800 345-6094 to spray a lab with a bleach
solution (Clidex?). This was messier than the formaldehyde, but probably
safer.
Those all happened soon after I started here. More recently, thanks to
advise from people on this list, I've been able to talk people out of
"nuking" rooms, which is especially important in our older buildings that
might recirculate air. Instead, they have to clean, dust and wash walls &
shelves with detergents.
Francis
Alcohol and calculus don't mix. Never drink and derive.
Francis Churchill, IHIT
University of Vermont - Environmental Safety Facility
655D Spear Street, Burlington, VT 05405-3010
(802) 656-5405
fchurchi@zoo.uvm.edu
=========================================================================
Date: Fri, 9 Apr 1999 08:36:59 +0100
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stuart Thompson
Subject: Re: Lab Cleaning Procedures Needed
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Francis Churchill
Sent: 08 April 1999 19:45
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: Lab Cleaning Procedures Needed
We had a similar issue when a mamalian tissue culture lab was moving into a
lab which had been used for mycology; the new researcher was concerned and
wanted to have the room decontaminated.
Another time we used ENV Services 800 345-6094 to spray a lab with a bleach
solution (Clidex?). This was messier than the formaldehyde, but probably
safer.
Be very careful with Cidex. Although its active ingredient, glutaraldehyde,
is an excellent disinfectant, it is, unfortunately, a strong irritant and
respiratory sensitizes.
One approach is to carry out all amplification and manipulation procedures
within a cabinet specially designed for PCR work. These are commercially
available and are not the same as microbiological safety cabinets. They
contain a strong UV source for inactivating any nucleic acid contamination
that enters the cabinet. If work is carried out in an open laboratory, its
contamination level will gradually rise, and repeated decontamination may be
necessary.
For advice on good working practices for PCR work, I suggest that you
contact a lab that carries out genetic analysis for the police and other
forensic matters (e.g. to settle questions of paternity). They have to be
extremely thorough so that their results stand up to examination in courts
of law.
Best wishes
Stuart
Dr Stuart Thompson
Health & Safety Services
University of Manchester
Waterloo Place
182/184 Oxford Road
Manchester M13 9PG
tel: +44 (0)161 275 5069
fax: +44 (0)161 275 6989
=========================================================================
Date: Fri, 9 Apr 1999 09:20:24 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janet Ives
Subject: lab waste
MIME-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
Good Morning Everyone,
We have been revisiting our waste disposal guidelines recently and
I have been charged with the task of gathering information regarding
lab waste generated from biosafety level 1 experiments. Are you
decontaminating this waste before disposal? We follow the BMBL
pretty strictly which says "All cultures, stocks, and other regulated
wastes are decontaminated before disposal by an approved
decontamination method, such as autoclaving. etc,etc" If this
waste is not considered infectious for humans, what is the rationale
for decontaminating prior to disposal?
Thanks in advance.
Janet
Janet Ives, Industrial Hygienist
University Risk Management and Environmental Safety
University of Rochester
300 East River Rd. room 23
Rochester, New York 14623
jives@safety.rochester.edu
(716)275-3014 Voice
(716)256-3155 Fax
=========================================================================
Date: Fri, 9 Apr 1999 10:22:40 +0500
Reply-To: speaker@ehs.psu.edu
Sender: A Biosafety Discussion List
From: Curt Speaker
Organization: UNIVERSITY SAFETY
Subject: Re: lab waste
In-Reply-To:
Janet:
I think many of us take the "better safe than sorry" approach to lab
waste. In other words, I would rather take the time and have
someone autoclave the material rather than be called by the landfill
operator and try and explain what these petri dishes, tubes, etc.
are doing in his landfill and why they are nothing to be worried
about.
Also, at least here at Penn State, much of the BL1 work is
recombinant DNA, which requires decontamination of waste before
disposal (as per NIH guidelines).
I would rather deal with it at the source than be called to the landfill
to prove that the material is non-hazardous.
My opinions are based partly in scientific fact, partly in practicality,
and partly from the Pennsylvania Dept. of Environmental Protection
regulations for infectious waste.
Just my $0.02...
Curt
Curt Speaker
Biosafety Officer
Penn State University
Environmental Health and Safety
speaker@ehs.psu.edu
^...^
(O_O)
=(Y)=
"""
=========================================================================
Date: Fri, 9 Apr 1999 10:57:20 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Frank A. Cantone"
Subject: Re: lab waste
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Janet,
I believe there are two issues. It is possible that certain biosafety
level 1 organisms can present themselves as opportunistic pathogens given
the "right" time and the appropriate individual (e.g., immunosuppressed).
Secondly, the unsuspecting custodian as well as people further up the
disposal chain may undergo unnecessary distress should they contact these
live cultures, unknowing if they are pathogenic or benign. Therefore, I
consider it good practice to decontaminate all biological waste prior to
disposal.
Frank
Frank A. Cantone, PhD
Assistant Biological Safety Officer
Department of Environmental Health & Safety
Cornell University
125 Humphreys Service Building
Ithaca, NY 14853
phone 607-254-4888
fax 607-255-8267
=========================================================================
Date: Fri, 9 Apr 1999 08:55:52 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: lab waste
MIME-Version: 1.0
Content-Type: text/plain
Hi, Janet -
Our Medical Waste Management Plan at UCSF defines biohazardous waste as,
among other things, "... wastes from the production of bacteria,
viruses, spores ... and culture dishes and devices used to transfer,
inoculate and mix cultures." We don't differentiate between RG1 and
other microorganisms with respect to disposal requirements, and we do
require that all biohazardous wastes be properly disposed. That means
either by incineration or by autoclaving in a QCed 'clave with
subsequent discard as solid (i.e., non-infectious) waste (aka "trash").
Part of our rationale here is to avoid the problems others have
described - upsetting people who may find plates etc. in the regular
trash, and especially upsetting our solid waste contractor, who has no
hesitancy at all about calling us to come to the transfer station and go
through an entire truckload of trash when they find something
suspicious. Another part is that since we have a fairly complex medical
waste stream, I want to avoid making waste stream segregation any more
complex than necessary - it's easier and less confusing if everyone
treats "red bag waste" the same, regardless of its source and associated
Risk Groups. The only variation is when the stuff originates in one of
our BSL3 labs, in which case it must be autoclaved for removal from the
lab.
That's how we do it here and it works OK for us.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Janet Ives [mailto:jives@SAFETY.ROCHESTER.EDU]
Sent: Friday, April 09, 1999 6:20 AM
To: BIOSAFTY@mitvma.mit.edu
Subject: lab waste
Good Morning Everyone,
We have been revisiting our waste disposal guidelines recently and
I have been charged with the task of gathering information regarding
lab waste generated from biosafety level 1 experiments. Are you
decontaminating this waste before disposal? We follow the BMBL
pretty strictly which says "All cultures, stocks, and other regulated
wastes are decontaminated before disposal by an approved
decontamination method, such as autoclaving. etc,etc" If this
waste is not considered infectious for humans, what is the rationale
for decontaminating prior to disposal?
Thanks in advance.
Janet
Janet Ives, Industrial Hygienist
University Risk Management and Environmental Safety
University of Rochester
300 East River Rd. room 23
Rochester, New York 14623
jives@safety.rochester.edu
(716)275-3014 Voice
(716)256-3155 Fax
=========================================================================
Date: Fri, 9 Apr 1999 08:30:51 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Alfred L. 'Al' Jin"
Subject: Re: lab waste
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Janet,
I would also like to add to Frank's comment below. Thirdly, one needs to
prevent any foreseeable impact on the environment. Although the RG1 agents
are not pathogenic to man, it may have a detrimental impact on the
enivronment and other life forms. Sewage treatment plants, bird
sanctuaries, composting plants and other industrial operation need to be
protected.
The issue of waste disposal have been discussed thoroughly in the 70's and
80's. EPA Guide for Infectious Waste Management (PB86-199130) dated May
1986, and ATSDR (Agency for Toxic Substances and Disease Registry's Public
Health Implication of Medical Waste: A Report to Congress (PB91-100271) are
2 documents that summarizes the ramifications of improperly disposing of
biological waste. Both documents refer to the RCRA Act of 1976 ahd the
"Cradle to Grave" management system.
In closing, I hope this helps.
Al Jin, BSO, IH, CBSP, BSM(ASM), MS, CM(ACM), M(ASCP),
Lawrence Livermore National Laboratory, Livermore, CA,
Hazards Control Department, MS-289,
925 423-7385, E-mail:Jin2@
>Janet,
>
>I believe there are two issues. It is possible that certain biosafety
>level 1 organisms can present themselves as opportunistic pathogens given
>the "right" time and the appropriate individual (e.g., immunosuppressed).
>Secondly, the unsuspecting custodian as well as people further up the
>disposal chain may undergo unnecessary distress should they contact these
>live cultures, unknowing if they are pathogenic or benign. Therefore, I
>consider it good practice to decontaminate all biological waste prior to
>disposal.
>
>Frank
>
>
>
>
>
>
>Frank A. Cantone, PhD
>Assistant Biological Safety Officer
>Department of Environmental Health & Safety
>Cornell University
>125 Humphreys Service Building
>Ithaca, NY 14853
>
>phone 607-254-4888
>fax 607-255-8267
=========================================================================
Date: Fri, 9 Apr 1999 13:21:21 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Susan Souder
Subject: Anti room to BL-3
MIME-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: 7bit
Hi,
I have a quick question. I know the BL-3 laboratory must be neg to the
outside. Now, the anti room should also be neg to the outside hall. Am
I correct? Can the anti room be Pos to the hallway, outside? I am
thinking not. I have maintenance telling me it has always been that way
so I need some reinforcement to get it fixed. Thank you very much. You
make reply to me.
Susan Souder, M.S., MT,(ASCP)
Biological Safety Officer
Thomas Jefferson University
Phila., Pa. 19107
215-503-7422
email susan.souder@mail.tju.edu
=========================================================================
Date: Fri, 9 Apr 1999 10:33:34 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: Anti room to BL-3
MIME-Version: 1.0
Content-Type: text/plain
Hi, Susan -
As a minimum, the anteroom should be negative to the corridor (outside)
and the lab negative to the anteroom. This will ensure that an airborne
agent that escapes from the lab into the anteroom stays in and is
ultimately exhausted from the anteroom without entering the corridor.
Airflow from the ourside space into any part of a containment lab suite
should always be inward. I'm not aware of any regulations that require
this, only recommendations in many design guides. Your common sense is
right here.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Susan Souder [mailto:Susan.Souder@MAIL.TJU.EDU]
Sent: Friday, April 09, 1999 10:21 AM
To: BIOSAFTY@mitvma.mit.edu
Subject: Anti room to BL-3
Hi,
I have a quick question. I know the BL-3 laboratory must be neg to the
outside. Now, the anti room should also be neg to the outside hall. Am
I correct? Can the anti room be Pos to the hallway, outside? I am
thinking not. I have maintenance telling me it has always been that way
so I need some reinforcement to get it fixed. Thank you very much. You
make reply to me.
Susan Souder, M.S., MT,(ASCP)
Biological Safety Officer
Thomas Jefferson University
Phila., Pa. 19107
215-503-7422
email susan.souder@mail.tju.edu
=========================================================================
Date: Fri, 9 Apr 1999 15:13:18 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janet Ives
Subject: herpes B
MIME-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
Thanks to you all that helped me out with why BSL-1 waste must
be deconned prior to disposal.
I (of course) have another question. This time it is about PPE for
non-human primate handlers. I have asked that our handlers be
fitted for an N95 for respiratory protection. Our N95s are fluid
resistant and therefore makes good nose/mouth protection as well.
The Occ Health physicians want to know who else has specific a
respirator.
So....Who out there has specified respiratory protection and is
using an N95?
Oh...our animals are screened for TB before they enter the facility.
No TB infected animals are allowed. We have provided other PPE
(bite/scratch resistant stuff, clothing covers, goggles, face shields,
shoe covers, gloves)
Thanks and happy weekend everyone.
Janet
Janet Ives, Industrial Hygienist
University Risk Management and Environmental Safety
University of Rochester
300 East River Rd. room 23
Rochester, New York 14623
jives@safety.rochester.edu
(716)275-3014 Voice
(716)256-3155 Fax
=========================================================================
Date: Fri, 9 Apr 1999 12:29:38 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: herpes B
MIME-Version: 1.0
Content-Type: text/plain
Hi Again, Janet -
At UCSF, we require all persons entering an Old World primate room or
working with the animals in any other location to wear head and shoe
covers, full-closure gown, gloves and a full face shield. We also
provide goggles and a surgical mask for wear beneath the shield but
don't require them. The agents of concern, other than TB (which you've
ruled out), are spread by contact and we want to protect all exposed
mucous membranes and potentially non-intact skin surfaces. We don't
feel that respiratory protection is indicated. I realize that many
other BSOs will disagree with me on this.
Requirements for working with New World primates are slightly less
rigorous because the agents they're known to harbor pose a lower risk.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Janet Ives [mailto:jives@SAFETY.ROCHESTER.EDU]
Sent: Friday, April 09, 1999 12:13 PM
To: BIOSAFTY@mitvma.mit.edu
Subject: herpes B
Thanks to you all that helped me out with why BSL-1 waste must
be deconned prior to disposal.
I (of course) have another question. This time it is about PPE for
non-human primate handlers. I have asked that our handlers be
fitted for an N95 for respiratory protection. Our N95s are fluid
resistant and therefore makes good nose/mouth protection as well.
The Occ Health physicians want to know who else has specific a
respirator.
So....Who out there has specified respiratory protection and is
using an N95?
Oh...our animals are screened for TB before they enter the facility.
No TB infected animals are allowed. We have provided other PPE
(bite/scratch resistant stuff, clothing covers, goggles, face shields,
shoe covers, gloves)
Thanks and happy weekend everyone.
Janet
Janet Ives, Industrial Hygienist
University Risk Management and Environmental Safety
University of Rochester
300 East River Rd. room 23
Rochester, New York 14623
jives@safety.rochester.edu
(716)275-3014 Voice
(716)256-3155 Fax
=========================================================================
Date: Fri, 9 Apr 1999 14:59:29 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Heather H. Gonsoulin"
Subject: Re: herpes B
MIME-Version: 1.0
Content-Type: multipart/mixed; boundary="---- =_NextPart_000_01BE8299.92D82AC0"
------ =_NextPart_000_01BE8299.92D82AC0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: quoted-printable
Hi Janet,
At NIRC we require only surgical masks (not N95 because we are mostly =
trying to protect our animals from getting human stuff), gloves, long =
sleeve garments, rubber boots, some type of eye protection (either =
goggles or safety glasses), and head covers. The only time we require =
N95 respirators is when we get a shipment of quarantine animals or =
during a necropsy or dental procedure (the later two because of the =
increase in airborne contaminants). We also require our employees to =
dress into uniforms and shower out at the end of the day.
Hope this helps.
Heather H. Gonsoulin
Occupational Health and Safety Officer
USL-NIRC
(318) 482-0306 (fax) 318-373-0057
hah8377@usl.edu
-----Original Message-----
From: Funk, Glenn [SMTP:GLENNF@EHSMAIL.UCSF.EDU]
Sent: Friday, 09 April, 1999 2:30 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: herpes B
Hi Again, Janet -
At UCSF, we require all persons entering an Old World primate room or
working with the animals in any other location to wear head and shoe
covers, full-closure gown, gloves and a full face shield. We also
provide goggles and a surgical mask for wear beneath the shield but
don't require them. The agents of concern, other than TB (which you've
ruled out), are spread by contact and we want to protect all exposed
mucous membranes and potentially non-intact skin surfaces. We don't
feel that respiratory protection is indicated. I realize that many
other BSOs will disagree with me on this.
Requirements for working with New World primates are slightly less
rigorous because the agents they're known to harbor pose a lower risk.
-- Glenn
-----------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
U.C. San Francisco
415-476-2097 fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Janet Ives [mailto:jives@SAFETY.ROCHESTER.EDU]
Sent: Friday, April 09, 1999 12:13 PM
To: BIOSAFTY@mitvma.mit.edu
Subject: herpes B
Thanks to you all that helped me out with why BSL-1 waste must
be deconned prior to disposal.
I (of course) have another question. This time it is about PPE for
non-human primate handlers. I have asked that our handlers be
fitted for an N95 for respiratory protection. Our N95s are fluid
resistant and therefore makes good nose/mouth protection as well.
The Occ Health physicians want to know who else has specific a
respirator.
So....Who out there has specified respiratory protection and is
using an N95?
Oh...our animals are screened for TB before they enter the facility.
No TB infected animals are allowed. We have provided other PPE
(bite/scratch resistant stuff, clothing covers, goggles, face shields,
shoe covers, gloves)
Thanks and happy weekend everyone.
Janet
Janet Ives, Industrial Hygienist
University Risk Management and Environmental Safety
University of Rochester
300 East River Rd. room 23
Rochester, New York 14623
jives@safety.rochester.edu
(716)275-3014 Voice
(716)256-3155 Fax
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=========================================================================
Date: Fri, 9 Apr 1999 16:37:55 -0400
Reply-To: jkeene@
Sender: A Biosafety Discussion List
From: "John H. Keene"
Subject: Re: lab waste
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Janet, I've read all the replys that your note has generated and it seems to
me that we all ought to read our local/state waste regulations. If the reg
is based on the EPA guide, then "all cultures and stocks" are to be handled
as infectious and either deconned or disposed of as regulated waste. In
fact Cultures and Stocks are considered to be regulated waste. (Wherever
they regulate such waste.) There is no mention of the Biosafety Level or
Risk Group. It is just good microbiological laboratory practice to
decontaminate cultures prior to releasing them to the waste stream.
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Janet Ives
Sent: Friday, April 09, 1999 9:20 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: lab waste
Good Morning Everyone,
We have been revisiting our waste disposal guidelines recently and
I have been charged with the task of gathering information regarding
lab waste generated from biosafety level 1 experiments. Are you
decontaminating this waste before disposal? We follow the BMBL
pretty strictly which says "All cultures, stocks, and other regulated
wastes are decontaminated before disposal by an approved
decontamination method, such as autoclaving. etc,etc" If this
waste is not considered infectious for humans, what is the rationale
for decontaminating prior to disposal?
Thanks in advance.
Janet
Janet Ives, Industrial Hygienist
University Risk Management and Environmental Safety
University of Rochester
300 East River Rd. room 23
Rochester, New York 14623
jives@safety.rochester.edu
(716)275-3014 Voice
(716)256-3155 Fax
=========================================================================
Date: Fri, 9 Apr 1999 14:24:52 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Alfred L. 'Al' Jin"
Subject: Re: Anti room to BL-3
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Susan,
The only ammunition you need is right in your own back yard. On page 5,
item #5 under "A BL-3 Facility: Special Features" of your (Thomas Jefferson
University) IBC's SOP for BL3, it states the following: "The entire BL-3
facility is under negative pressure relative to the other adjacent rooms(s)
and corridor(s) and hence air always flows into the containment facility
from the least contaminated area".
I hope this anwsers your question.
Al Jin, BSO, IH, CBSP, BSM(ASM), MS, CM(ACM), M(ASCP),
Lawrence Livermore National Laboratory, Livermore, CA,
Hazards Control Department, MS-289,
925 423-7385, E-mail:Jin2@
>Hi,
>I have a quick question. I know the BL-3 laboratory must be neg to the
>outside. Now, the anti room should also be neg to the outside hall. Am
>I correct? Can the anti room be Pos to the hallway, outside? I am
>thinking not. I have maintenance telling me it has always been that way
>so I need some reinforcement to get it fixed. Thank you very much. You
>make reply to me.
>
>Susan Souder, M.S., MT,(ASCP)
>Biological Safety Officer
>Thomas Jefferson University
>Phila., Pa. 19107
>215-503-7422
>email susan.souder@mail.tju.edu
=========================================================================
Date: Mon, 12 Apr 1999 13:07:02 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Winston Longue
Subject: health screenings...
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
To the list,
I am interested to know if anyone out there who may be familiar with the
occupational hazard involved with workers who deal with autoclaving
machines with regards to the fumes that they breathe in. Some of them have
complained of headaches. Is there anything they should be alarmed of? If
they are sent for medical check ups, what exactly should they be checking
up for. The obvious would be to screen for something respiratorial, but
anyone know specifcally what. Is it neccessary for this to be a mandatory
check up.
TIA for any help.
Regards,
Winston
--------------------------------------------------------------------
Winston Longue
Safety Officer
Institute of Molecular and Cell Biology,
National University of Singapore.
mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Mon, 12 Apr 1999 04:50:27 EDT
Reply-To: EKrisiunas@
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Lab waste
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
It has been quite interesting to read all the responses regarding treatment
of cultures and stocks on-site before sending them off-site as solid waste or
for further treatment. Dr. Keene is correct to check your local and state
regulations. Forget EPA for the time being unless you operate a medical waste
incinerator. USDOT is also interested in waste cultures and stocks but from
a packaging standpoint. A majority of hospital basic clinical microbiology
labs have stopped autoclaving their waste due to economics - why autoclave
when I can send it off-site to be microwave, macrowave, steam, or
incinerated. I know of only a few jurisdictions in the US where such practice
is required by law - New York City and South Carolina. Only 21% of clinical
labs outside of NYC still autoclave their waste cultures and stocks -
presented last year at ABSA (Survey done by Dr. Salkin , Dr. Woodard, Sally
Patterson - NYSDOH and myself ).
So while many of you state it is good lab practice, and I strongly support
it, it appears that many manage their waste differently.
Regards,
Ed Krisiunas, MT(ASCP), CIC, MPH
Director
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
=========================================================================
Date: Mon, 12 Apr 1999 07:28:21 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: Anti room to BL-3
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Both Al and Glen are right, but good engineering design - if done right and
monitored - can allow other anteroom set ups and still offer containment.
I have pharsed it in some of our documents, as below. I prefer the first
set up - "clean anteroom" which offers an "air barrier or air lock" between
public areas and containment facilities. People exiting and entering
through the air lock must close the door and wait for the pressure
differential to re-establish before opening the next door, coming and
going. We use alarmed differnetial prssure gauges (not magnehelic types) -
which there are several types available on the market now. The first 2
designs offer both product protection and containment and we like to use
them, when we can get them, for FDA approved IND trials. The third option,
which would be like Al and Glen mention - straight through neg pressure is
also an option, it offers containment, but doesn't offer product protection
in the processing room outside of the BSCs. Keep in mind that pentrations
must be sealed in BL3 labs - if not and your sucking or blowing - you're
just going to suck in or push out dirty air through cracks, regardless of
the pressure differential across the entry doors.
1. CLEAN ANTEROOM + CLEAN PROCESS ROOM DESIGN
The Processing area is under negative pressure with HEPA filtered air
supply and has a 2-door Anteroom entrance with a HEPA filtered positive
air supply [air- lock] for both containment, and product
protection/separation 100 ng/kg. The botulinum toxins have LD 50s ranging from 0.4 -2.5
ng/kg.
Thanks.
Cheri Marcham, CIH, CSP, CHMM
The University of Oklahoma Health Sciences Center
cheri-marcham@ouhsc.edu
(405) 271-3000
FAX (405) 271-1606
=========================================================================
Date: Wed, 21 Apr 1999 10:35:54 -0500
Reply-To: "mkinsey@"
Sender: A Biosafety Discussion List
From: Melina Kinsey
Organization: MRI
Subject: Tracking Biological Materials
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
I was wondering how others in the group are handling the task of tracking
biological materials or toxins that are on the CDC Select Agent list. After
you receive the material in, do you track the storage location and destruction
of:
1. the original material only
2. all prodigy and the original
Secondly, how do you do this? I know this is a weighted question, but I am
having great difficulty in establishing a protocol that isn't too cumbersome to
the technical staff.
If you have a complex of laboratories, all registered for the material, do you
specify the storage location as the laboratory or the complex.
Any help you can offer me, would be greatly appreciated. We have been
struggling with the technical staff to define something that would please an
auditor, and our security and safety staff.
TIA
Melina Kinsey
Biosafety Officer
Midwest Research Institute
425 Volker Blvd.
Kansas City, MO 64110
(815) 753-7600 x1424
mkinsey@
=========================================================================
Date: Thu, 22 Apr 1999 16:11:59 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Winston Longue
Subject: biological spill kit
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
to the list,
anyone aware from whom (which company)and where i can purchase a biological
spill kit. As I am in singapore, i need to know perhaps the web address of
the company and from there on, trace the local agent here in singapore.
Anyone with any suggestions?
Thanks in advance for any help on this.
Winston
--------------------------------------------------------------------
Winston Longue
Safety Officer
Inst.of Molecular and Cell Biology (IMCB)
30 Medical Drive
Singapore 117609
Tel: (65) 874-7888
mcbwel@imcb.nus.edu.sg
=========================================================================
Date: Thu, 22 Apr 1999 09:29:57 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: biological spill kit
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hi Winston,
Lab Safety Supply is a good reference for you. They are at
or 1-800-356-0783.
At 04:11 PM 4/22/99 +0800, you wrote:
>to the list,
>
>anyone aware from whom (which company)and where i can purchase a biological
>spill kit. As I am in singapore, i need to know perhaps the web address of
>the company and from there on, trace the local agent here in singapore.
>Anyone with any suggestions?
>Thanks in advance for any help on this.
>
>Winston
>--------------------------------------------------------------------
>Winston Longue
>Safety Officer
>Inst.of Molecular and Cell Biology (IMCB)
>30 Medical Drive
>Singapore 117609
>
>Tel: (65) 874-7888
>mcbwel@imcb.nus.edu.sg
>
>
Janice
=========================================================================
Date: Thu, 22 Apr 1999 10:24:32 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: biological spill kit
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Winston:
The best spill kit can be obtained from K mart or your local grocery
store. It contains fresh bleach in the smaller bottle size, some thick mil
garbage bags, a cheap plastic dust pan, a small plastic broom, a pack of
absorbant baby diapers, a pair of cheap metal or plastic tongs, some
disposable plastic food containers with lids, a couple of biohazard
signs and/ or biohazard tape, a 1/2 dozenlabeled biohazard red bags
[from work], some PPE [ two disposable lab coats, dish washer rubber gloves,
maybe some disposable foot covers, several pairs of disposable latex gloves,
two pair of disposable safety glasses, two surgical or paint face masks
to protect against splatter to mucous membranes], a small plastic bucket,
dish washing soap concentrate, a small disposable mop, a roll of paper hand
towels, a printed copy of your facility's "spill clean up procedure" and
spill report form in a zip lock bag clearly labeled as such on the outside,
a sterile 50 cc orange top centrifuge tube, a sharpee lab ink pen, some qt
sized zip lock bags, one or two plastic bottles of local drinking water
and 12 "twistems" all placed in a biohazard, leak resistant disposable carton
for used for biohazard waste incineration from your local medical waste
hauler.
Quiz: Now tell me what each item listed is potentially useful for in the
management of a truly biohazardous spill situation in the typical BSL-2
containment lab or in a vehicle legally transporting say human blood
samples in the trunk as many clinical labs do? Everybody take the test
and add items I probably forgot. Cost, about $30 or less.
My opinion!
Joe Coggin, Jr. Ph.D. RBP, CBSP
On Thu, 22 Apr 1999, Winston Longue wrote:
> to the list,
>
> anyone aware from whom (which company)and where i can purchase a biological
> spill kit. As I am in singapore, i need to know perhaps the web address of
> the company and from there on, trace the local agent here in singapore.
> Anyone with any suggestions?
> Thanks in advance for any help on this.
>
> Winston
> --------------------------------------------------------------------
> Winston Longue
> Safety Officer
> Inst.of Molecular and Cell Biology (IMCB)
> 30 Medical Drive
> Singapore 117609
>
> Tel: (65) 874-7888
> mcbwel@imcb.nus.edu.sg
>
=========================================================================
Date: Thu, 22 Apr 1999 12:13:17 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: biological spill kit
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Winston,
Don't kill yourself over this. Draw up wish list of what you want.
contact a local Janitorial supply house. They probably have everything you
want. Have them fill your order. They may even have the kit already made
up. This is very possible if the supply hospitals in the area.
This offers several advantages:
They are local
They are cheap. I do order from places like lab safety and supply but I ty
not to. I consider them horribly over priced.
They can respond very fast if you need it in a hurry.
Bob
>
>
>On Thu, 22 Apr 1999, Winston Longue wrote:
>
>> to the list,
>>
>> anyone aware from whom (which company)and where i can purchase a biological
>> spill kit. As I am in singapore, i need to know perhaps the web address of
>> the company and from there on, trace the local agent here in singapore.
>> Anyone with any suggestions?
>> Thanks in advance for any help on this.
>>
>> Winston
>> --------------------------------------------------------------------
>> Winston Longue
>> Safety Officer
>> Inst.of Molecular and Cell Biology (IMCB)
>> 30 Medical Drive
>> Singapore 117609
>>
>> Tel: (65) 874-7888
>> mcbwel@imcb.nus.edu.sg
>>
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Fri, 23 Apr 1999 09:26:27 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Question : Biosafety Manuals
MIME-Version: 1.0
Content-Type: text/plain
Question regarding Biosafety Manuals:
We currently have hard (in every lab) and electronic (on everyone's desk
top) copies of our Biosafety Manual.
The electronic copies are up to date and the hard copies are getting there!
My question - does anyone know of a particular reg or requirement for hard
copies of the manual? The NRC has stated that they are not up to that level
of technology and would prefer the regs are followed for hard copies.
I know we are required to have access to the safety manual and if we have
the electronic copy on everyone's computer and a few hard copies for back up
in case of computer failure- should that not suffice?
Thank you and have a wonderful weekend!
Bliss M. Schlank
Biosafety Specialist
Zeneca Pharmaceuticals
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185
Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Fri, 23 Apr 1999 06:47:19 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Tackett
Subject: Re: Question : Biosafety Manuals
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
I am not aware of any reg that would require you to
use hard copy. One step to take would be to make sure
(and document) that everyone has been instructed about
accessing the electronic copy and that they know where
the hard copy back-up is located
_________________________________________________________
Do You Yahoo!?
Get your free @ address at
=========================================================================
Date: Mon, 26 Apr 1999 08:12:25 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: New Laboratory Safety Web Site
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
FYI:
>
>Acid spills, fires, and other hazards are everyday concerns
>in a modern laboratory. Learning how to react in the face
>of these situations is the purpose of a new laboratory
>training Web site "Knowing How to Practice Safe Science."
>This new site, at ,
>shows students, teachers, and research scientists how to act
>safely in a laboratory, and then challenges them to react
>quickly when problems arise. The site can be easily adapted
>to assist an individual's interest and research, and includes
>an interactive quiz that rates the user's safe practice knowledge.
>
>The site was created for Howard Hughes Medical Institute
>(HHMI) researchers with the intent of making it available as a
>public service to all laboratory researchers.
>
>
>
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
=========================================================================
Date: Mon, 26 Apr 1999 18:57:20 +0000
Reply-To: gmerkle@pop.wright.edu
Sender: A Biosafety Discussion List
From: Greg Merkle
Subject: Responsibility of a University Institutional Biosafety Committee
MIME-version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
Seeking information on oversight responsiblities of university
Institutional Biosafety Committee on activities in teaching
laboratories. Does the IBC get involved in reviewing activities? If
involved what format used for overseeing, if not involved why not?
Suggestions of web pages/sites for examples of operating programs.
Your help and insights are greatly appreciated. Thanks.
=========================================================================
Date: Tue, 27 Apr 1999 15:54:34 -0400
Reply-To: hontzb3@SERVAL.UOFS.EDU
Sender: A Biosafety Discussion List
From: Barbara Hontz
Organization: University of Scranton
Subject: Safety glasses/goggles in biology labs
MIME-version: 1.0
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Hello! My name is Barbara Hontz, and I am from the University of
Scranton in Pennsylvania. After having a student get splashed in the eye
with "cat juice" during a dissection, there have been questions about
wether students should be wearing safety glasses/goggles in biology lab.
This particular student was wearing contacts even though the students
are told not to.
I wanted to know the conventions at other institutions about this topic.
Are students required to wear safety glasses/goggles during general
biology labs?
Any advice on the topic would be greatly appreciated!
Barbara Hontz
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title: Lab Supervisor
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=========================================================================
Date: Wed, 28 Apr 1999 08:31:52 -0400
Reply-To: hontzb3@SERVAL.UOFS.EDU
Sender: A Biosafety Discussion List
From: Barbara Hontz
Organization: University of Scranton
Subject: Safety glasses
MIME-version: 1.0
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One more question if you will indulge me.
The overwhelming response has been YES! wear safety glasses (much to my
expectation), and now a faculty member asked me if regular eyeglasses
-without splash guards are sufficient. My first instinct is no, but as
you all know, documentation is everything.
Any other thoughts?
Thank you for your time!
Barbara Hontz
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=========================================================================
Date: Thu, 29 Apr 1999 07:41:42 -0500
Reply-To: "mkinsey@"
Sender: A Biosafety Discussion List
From: Melina Kinsey
Organization: MRI
Subject: Fogging Eyeware in Animal facilities
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Hi All!!
I am in need of help again. Our animal care workers, who clean the dog cages,
are having trouble seeing out of their eye protection, safety glasses and
goggles. They have tried a few antifogging products but have had no luck.
They tried a disposable face shield that only fell apart with water. Do any
of you have suggestions?
TIA
Melina Kinsey
Asst. Safety Officer
Midwest Research Institute
425 Volker Blvd.
Kansas City, MO 64110
(815) 753-7600 x1424
mkinsey@
=========================================================================
Date: Thu, 29 Apr 1999 22:56:51 EDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Genetically modified organisms
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Good evening:
Are there any standards or guidance on the disposal of genetically modified
organisms?
Are there any standards on the disposal of transgenic animals? Bury or
incinerate?
Ed Krisiunas, MT(ASCP), CIC, MPH
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
=========================================================================
Date: Fri, 30 Apr 1999 12:52:53 +0100
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stuart Thompson
Subject: Re: Genetically modified organisms
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Ed Krisiunas
Sent: 30 April 1999 03:57
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Genetically modified organisms
Good evening:
Are there any standards or guidance on the disposal of genetically modified
organisms?
In Manchester, England, we autoclave to make safe for transport across the
City to an incinerator. If we had an incinerator on site we would miss out
the autoclave step.
Are there any standards on the disposal of transgenic animals? Bury or
incinerate?
Incinerate
Best wishes
Stuart
Dr Stuart Thompson
Health & Safety Services
University of Manchester
Waterloo Place
182/184 Oxford Road
Manchester M13 9PG
tel: +44 (0)161 275 5069
fax: +44 (0)161 275 6989
Ed Krisiunas, MT(ASCP), CIC, MPH
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
=========================================================================
Date: Fri, 30 Apr 1999 16:19:21 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Jean.Goldberg"
Subject: Shipping Research Specimens
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; CHARSET=US-ASCII
I just received a call from a researcher who wants to ship
human blood and urine samples to another facility. She
knows the proper procedures for packaging and labeling the
specimens. However, she is very concerned because she has
not attended an IATA approved course on Dangerous Goods
Transportation. She is worried about being fined $10,000.
Question: Does she really need to attend an IATA approved
training course in order to be in compliance with the
regulations. Thanks in advance for any help you can
provide.
----------------------------------------
Jean Goldberg
Email: Jean.Goldberg@Med.Nyu.Edu
"NYU Medical Center"
=========================================================================
Date: Mon, 3 May 1999 09:39:35 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: Re: Shipping Research Specimens
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Jean
I don't think the shipper should attend the IATA course. In Hong Kong, I
always ask the airline and courier services company to help me. You must
declare the content of the shipment clearly. The airline, courier services,
and the forwarder will decide whether accept the samples or not. Also, you
must check the import and export regulation of both facilities and countries.
YK WAN
Safety Officer, CUHK, Hong Kong
At 04:19 PM 4/30/99 -0400, you wrote:
>I just received a call from a researcher who wants to ship
>human blood and urine samples to another facility. She
>knows the proper procedures for packaging and labeling the
>specimens. However, she is very concerned because she has
>not attended an IATA approved course on Dangerous Goods
>Transportation. She is worried about being fined $10,000.
>Question: Does she really need to attend an IATA approved
>training course in order to be in compliance with the
>regulations. Thanks in advance for any help you can
>provide.
>
>----------------------------------------
>Jean Goldberg
>Email: Jean.Goldberg@Med.Nyu.Edu
>"NYU Medical Center"
>
>
Please reply when you receive the message. Thank you.
***** Yu Kwan WAN,
***** Safety Officer
***** The Chinese University of Hong Kong
***** Shatin, NT, Hong Kong
***** Email:
***** ulsoykwan@cuhk.edu.hk
***** ulsoykwan@
***** ulsoykwan@
***** ulsoykwan@
=========================================================================
Date: Mon, 3 May 1999 07:42:32 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Lori Nicholson
Subject: Re: Shipping Research Specimens
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
I would definitely have the shipper get training to help them make certain
the paperwork and packaging is correct. A recent BOO BOO cost our company
$ 27,000.00 and it was a very small thing. We had SAF T PAK come and do a
course for our company recently. Very worth while. They also do public
seminars around the country and the cost is about $189.00 per person for a
one day training. Check their website for dates and cities. The airlines
are becoming very cautious with the threat of biological warfare looming.
The number of customs officers have been increased to help with looking for
packages that carry biologicals. Do not take any chances, get the training
and expect this to be an issue that will not be going away anytime soon.
Lori Nicholson
Madison WI
=========================================================================
Date: Mon, 3 May 1999 08:08:35 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Lori Nicholson
Subject: shipping
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
I would definitely have the shipper get training. Make certain
the paperwork and packaging is correct. A recent BOO BOO cost our company
$ 27,000.00 and it was a very small thing. We had SAF T PAK come and do a
course for our company recently. Very worth while. They also do public
seminars around the country and the cost is about $189.00 per person for a
one day training. Check their website for dates and cities. The airlines
are becoming very cautious with the threat of biological warfare looming.
The number of customs officers have been increased to help with looking for
packages that carry biologicals. Do not take any chances, get the training
and expect this to be an issue that will not be going away anytime soon.
Lori Nicholson
=========================================================================
Date: Mon, 3 May 1999 09:03:49 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Genetically modified organisms
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
At 10:56 PM 4/29/99 EDT, you wrote:
>Good evening:
>
>Are there any standards or guidance on the disposal of genetically modified
>organisms?
>
>Are there any standards on the disposal of transgenic animals? Bury or
>incinerate?
The guidelines for the US are in the NIH rDNA Guidelines. Recombinant orgs
have to be rendered nonviable. Animals should be incinerated.
Richie Fink
M.I.T.
rfink@mit.edu
=========================================================================
Date: Mon, 3 May 1999 09:32:59 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Gill Norton
Organization: University of Western Ontario
Subject: Re: Shipping Research Specimens
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Content-Transfer-Encoding: 7bit
IATA and TDG regulations require that the shipper of dangerous goods be
trained or be under the supervision of a trained person. ( IATA 6-1-1).
For an institution which has individual researchers shipping infectious
materials under UN 2814 or 2815, the individual should be trained or at
least have someone at the instition who has been trained to verify that
the shipment is send according to the regulations. The potential fines
are BIG. That said, I don't think too many places train all their
researchers in TDG. At my institution we are trying to get a central
department (OH&S) with TDG trained people that researchers can use as a
resource for shipping and to check packaging and paperwork etc. Without
this the instution is wide open for liability!
Jean.Goldberg wrote:
>
> I just received a call from a researcher who wants to ship
> human blood and urine samples to another facility. She
> knows the proper procedures for packaging and labeling the
> specimens. However, she is very concerned because she has
> not attended an IATA approved course on Dangerous Goods
> Transportation. She is worried about being fined $10,000.
> Question: Does she really need to attend an IATA approved
> training course in order to be in compliance with the
> regulations. Thanks in advance for any help you can
> provide.
>
> ----------------------------------------
> Jean Goldberg
> Email: Jean.Goldberg@Med.Nyu.Edu
> "NYU Medical Center"
--
------------------------------------------------------------------
Gillian Norton
Biosafety Officer
The University of Western Ontario
Occupational Health and Safety
Somerville House, Rm 2345
Phone: (519)661-2036
FAX: (519)661-3420
-------------------------------------------------------------------
=========================================================================
Date: Mon, 3 May 1999 09:15:24 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Shipping Research Specimens
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
At 04:19 PM 4/30/99 -0400, you wrote:
>I just received a call from a researcher who wants to ship
>human blood and urine samples to another facility. She
>knows the proper procedures for packaging and labeling the
>specimens. However, she is very concerned because she has
>not attended an IATA approved course on Dangerous Goods
>Transportation.
The DOT regs state that everyone who offers for shipment dangerous goods
must be trained. The type of training is not specified. In the short term
she can probably work with the shipper to ensure that the material is
packaged correctly and the paper work is done correctly but she will need
to be trained.
Richie Fink
M.I.T.
rfink@mit.edu
=========================================================================
Date: Mon, 3 May 1999 11:26:38 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Shipping Research Specimens
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
I have just taken over our shipping program here. I am attempting to
address several of the points you discuss and I have several more questions
to raise.
Training:
My department has been training our researchers for years on how to ship
specific items. I have descovered that nobody has kept records on who was
trained.
We now offer a sign-in sheet with our training which is filed in my
department. Attached to this sheet is a synopsis of what the training was
about. We do not train to ship everything. Instead we train for a
specific item. This allow us to cookbook the training. The group gives me
the names of the item(s) they intend to ship. I then research how to ship
these items only. They are trained on that subject only. The synopsis
includes packaging and all information from the CFR49 172.101 table. We
cover shipping papers, shipping containers, labels, markings and inner
containement for that item only. I also include research if the item is
being exported and the material is TSCA regulated. Haven't had one of
those yet.
Specific material training can take as little as 30 minutes. Any time a
different material is to be shipped, we must rain on the new material.
Now, I have two questions that I am researching at this time.
I have labs who are shipping human blood and blood products. These are not
diagnostic speciments. They are research specimens. Diagnostic specimens
is a Proper Shipping Name(PSN) that is exempt from several regulations.
We do not test for infectious substances. Since we are handling blood,
Universal Precautions and OSHA say to consider the material infectious.
DOT says properly identify the hazard. DO NOT over classify.
So should I ship human blood as "Infectious Substance, 6.2" or is it
unregulated?
Shipping containers:
I am encountering several domestic shipping containers that do not have the
proper markings to indicate that they are OK for hazardous materials.
Instead they are being "Certified" for freight shipment with the
specifications being listed. These containers are being provided to us
from other labs, some very prestigeous, and we are being asked to ship our
specimens in these containers. Are these containers acceptable?
Bob
>At 04:19 PM 4/30/99 -0400, you wrote:
>>I just received a call from a researcher who wants to ship
>>human blood and urine samples to another facility. She
>>knows the proper procedures for packaging and labeling the
>>specimens. However, she is very concerned because she has
>>not attended an IATA approved course on Dangerous Goods
>>Transportation.
>
>The DOT regs state that everyone who offers for shipment dangerous goods
>must be trained. The type of training is not specified. In the short term
>she can probably work with the shipper to ensure that the material is
>packaged correctly and the paper work is done correctly but she will need
>to be trained.
>
>Richie Fink
>M.I.T.
>rfink@mit.edu
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Mon, 3 May 1999 10:50:30 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: Re: Shipping Research Specimens
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Bob,
Human blood and blood products that have no or very low probability of
containing infectious disease agents do fall under the definition of
"Diagnostic Specimens" as defined for the proper shipping name. UN
certified packaging is not required, but they must be packed in accordance
with IATA packaging instruction 650, which odes require that the packaging
meet certain test criteria. The packing requirements are similar to those
for "Infectious Substances", except for the lack of requirement for UN
certified packaging.
Julie A. Johnson, Ph.D.
Biosafety Officer
Iowa State University
Environmental Health & Safety
118 Agronomy Lab
phone: 515-294-7657
fax: 515-294-9357
E-mail: jajohns@iastate.edu
-----Original Message-----
From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
Sent: Monday, May 03, 1999 11:27 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: Shipping Research Specimens
I have just taken over our shipping program here. I am attempting to
address several of the points you discuss and I have several more questions
to raise.
Training:
My department has been training our researchers for years on how to ship
specific items. I have descovered that nobody has kept records on who was
trained.
We now offer a sign-in sheet with our training which is filed in my
department. Attached to this sheet is a synopsis of what the training was
about. We do not train to ship everything. Instead we train for a
specific item. This allow us to cookbook the training. The group gives me
the names of the item(s) they intend to ship. I then research how to ship
these items only. They are trained on that subject only. The synopsis
includes packaging and all information from the CFR49 172.101 table. We
cover shipping papers, shipping containers, labels, markings and inner
containement for that item only. I also include research if the item is
being exported and the material is TSCA regulated. Haven't had one of
those yet.
Specific material training can take as little as 30 minutes. Any time a
different material is to be shipped, we must rain on the new material.
Now, I have two questions that I am researching at this time.
I have labs who are shipping human blood and blood products. These are not
diagnostic speciments. They are research specimens. Diagnostic specimens
is a Proper Shipping Name(PSN) that is exempt from several regulations.
We do not test for infectious substances. Since we are handling blood,
Universal Precautions and OSHA say to consider the material infectious.
DOT says properly identify the hazard. DO NOT over classify.
So should I ship human blood as "Infectious Substance, 6.2" or is it
unregulated?
Shipping containers:
I am encountering several domestic shipping containers that do not have the
proper markings to indicate that they are OK for hazardous materials.
Instead they are being "Certified" for freight shipment with the
specifications being listed. These containers are being provided to us
from other labs, some very prestigeous, and we are being asked to ship our
specimens in these containers. Are these containers acceptable?
Bob
>At 04:19 PM 4/30/99 -0400, you wrote:
>>I just received a call from a researcher who wants to ship
>>human blood and urine samples to another facility. She
>>knows the proper procedures for packaging and labeling the
>>specimens. However, she is very concerned because she has
>>not attended an IATA approved course on Dangerous Goods
>>Transportation.
>
>The DOT regs state that everyone who offers for shipment dangerous goods
>must be trained. The type of training is not specified. In the short term
>she can probably work with the shipper to ensure that the material is
>packaged correctly and the paper work is done correctly but she will need
>to be trained.
>
>Richie Fink
>M.I.T.
>rfink@mit.edu
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental
Safety
=========================================================================
Date: Mon, 3 May 1999 13:09:52 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Jean.Goldberg"
Subject: Cationic Liposomes
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; CHARSET=US-ASCII
First, thanks to all who responded to my question on
shipping research specimens. Based on your responses I
have decided that, at a minimum, I will provide researchers
with a Self-study Packet on this topic. Now for another
query. I have a researcher who would like to use cationic
liposomes mixed with plasmids to deliver genes for
beta-galactosidase and Plasminogen Activator Inhibitor-1 to
rabbits. I am not sure if this is BSL1 or BSL2 activity. I
would appreciate you thoughts. Thanks in advance. -- Jean
----------------------------------------
Jean Goldberg
Email: Jean.Goldberg@Med.Nyu.Edu
"NYU Medical Center"
=========================================================================
Date: Mon, 3 May 1999 10:30:16 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: Shipping Research Specimens
MIME-Version: 1.0
Content-Type: text/plain
It goes even further. There is a Federal requirement for formal
documented training at least every two years (per 49CFR172.704) for all
people in the US who ship dangerous goods, including biohazardous
materials. The Saf-T-Pak course is quite good and they are coming out
with a CD-ROM training course very soon that will probably meet the
Federal training requirement - check their web site at
. Even tho Saf-T-Pak is a Canadian company, they
are quite familiar with 49CFR requirements and anyway, most folks are
training to the IATA DGRs rather than 49CFR because meeting the DGRs
will satisfy the Feds' requirements.
------------------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
University of California, San Francisco
Voice 415-476-2097
Fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Lori Nicholson [mailto:Lori_Nicholson/Powderject@]
Sent: Monday, May 03, 1999 5:43 AM
To: BIOSAFTY@mitvma.mit.edu
Subject: Re: Shipping Research Specimens
I would definitely have the shipper get training to help them make
certain
the paperwork and packaging is correct. A recent BOO BOO cost our
company
$ 27,000.00 and it was a very small thing. We had SAF T PAK come and do
a
course for our company recently. Very worth while. They also do public
seminars around the country and the cost is about $189.00 per person for
a
one day training. Check their website for dates and cities. The
airlines
are becoming very cautious with the threat of biological warfare
looming.
The number of customs officers have been increased to help with looking
for
packages that carry biologicals. Do not take any chances, get the
training
and expect this to be an issue that will not be going away anytime soon.
Lori Nicholson
Madison WI
=========================================================================
Date: Mon, 3 May 1999 13:02:25 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: Cationic Liposomes
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
I'd go for BSL1 - they can not pass from host to secondary person. The
only exception I would make is if it is a highly toxic gene insert in the
plasmid as per NIH rDNA guide. Even with an oncogene insert like Kras, it
may require IBC review, but probably still no BSL 2 special handling needed
unless a novel method of administration is proposed. We did have one PI
who was sending L-plasmids through a SPAG (small particle aerosol
generator) then administering the mist to rabbits via a face mask. We took
special precautions in that case - and set him up inside of a BSC. Judy
Pointer, MDACC
"Jean.Goldberg" on 05/03/99 12:09:52 PM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: Cationic Liposomes
First, thanks to all who responded to my question on
shipping research specimens. Based on your responses I
have decided that, at a minimum, I will provide researchers
with a Self-study Packet on this topic. Now for another
query. I have a researcher who would like to use cationic
liposomes mixed with plasmids to deliver genes for
beta-galactosidase and Plasminogen Activator Inhibitor-1 to
rabbits. I am not sure if this is BSL1 or BSL2 activity. I
would appreciate you thoughts. Thanks in advance. -- Jean
----------------------------------------
Jean Goldberg
Email: Jean.Goldberg@Med.Nyu.Edu
"NYU Medical Center"
=========================================================================
Date: Mon, 3 May 1999 14:52:54 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Shipping Research Specimens
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Okay,
Now is there an appropriate Proper Shipping Name?
Bob
>Bob,
>Human blood and blood products that have no or very low probability of
>containing infectious disease agents do fall under the definition of
>"Diagnostic Specimens" as defined for the proper shipping name. UN
>certified packaging is not required, but they must be packed in accordance
>with IATA packaging instruction 650, which odes require that the packaging
>meet certain test criteria. The packing requirements are similar to those
>for "Infectious Substances", except for the lack of requirement for UN
>certified packaging.
>
>Julie A. Johnson, Ph.D.
>Biosafety Officer
>Iowa State University
>Environmental Health & Safety
>118 Agronomy Lab
>phone: 515-294-7657
>fax: 515-294-9357
>E-mail: jajohns@iastate.edu
>
>
>
>
>-----Original Message-----
>From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
>Sent: Monday, May 03, 1999 11:27 AM
>To: BIOSAFTY@MITVMA.MIT.EDU
>Subject: Re: Shipping Research Specimens
>
>
>I have just taken over our shipping program here. I am attempting to
>address several of the points you discuss and I have several more questions
>to raise.
>
>Training:
>
>My department has been training our researchers for years on how to ship
>specific items. I have descovered that nobody has kept records on who was
>trained.
>
>We now offer a sign-in sheet with our training which is filed in my
>department. Attached to this sheet is a synopsis of what the training was
>about. We do not train to ship everything. Instead we train for a
>specific item. This allow us to cookbook the training. The group gives me
>the names of the item(s) they intend to ship. I then research how to ship
>these items only. They are trained on that subject only. The synopsis
>includes packaging and all information from the CFR49 172.101 table. We
>cover shipping papers, shipping containers, labels, markings and inner
>containement for that item only. I also include research if the item is
>being exported and the material is TSCA regulated. Haven't had one of
>those yet.
>
>Specific material training can take as little as 30 minutes. Any time a
>different material is to be shipped, we must rain on the new material.
>
>Now, I have two questions that I am researching at this time.
>
>I have labs who are shipping human blood and blood products. These are not
>diagnostic speciments. They are research specimens. Diagnostic specimens
>is a Proper Shipping Name(PSN) that is exempt from several regulations.
>
>We do not test for infectious substances. Since we are handling blood,
>Universal Precautions and OSHA say to consider the material infectious.
>DOT says properly identify the hazard. DO NOT over classify.
>
>So should I ship human blood as "Infectious Substance, 6.2" or is it
>unregulated?
>
>Shipping containers:
>I am encountering several domestic shipping containers that do not have the
>proper markings to indicate that they are OK for hazardous materials.
>Instead they are being "Certified" for freight shipment with the
>specifications being listed. These containers are being provided to us
>from other labs, some very prestigeous, and we are being asked to ship our
>specimens in these containers. Are these containers acceptable?
>
>Bob
>
>
>>At 04:19 PM 4/30/99 -0400, you wrote:
>>>I just received a call from a researcher who wants to ship
>>>human blood and urine samples to another facility. She
>>>knows the proper procedures for packaging and labeling the
>>>specimens. However, she is very concerned because she has
>>>not attended an IATA approved course on Dangerous Goods
>>>Transportation.
>>
>>The DOT regs state that everyone who offers for shipment dangerous goods
>>must be trained. The type of training is not specified. In the short term
>>she can probably work with the shipper to ensure that the material is
>>packaged correctly and the paper work is done correctly but she will need
>>to be trained.
>>
>>Richie Fink
>>M.I.T.
>>rfink@mit.edu
>
>
> ________________________________________________
>__ / _______________________________________________
>_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
>Safety
> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
> \ / U.S.A. RA Member Environmental
>Safety
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Mon, 3 May 1999 15:06:08 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stefan Wagener
Subject: Shipping & Training on the Web!
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Since this training topic (Shipping of infectious agents) is of
considerable interest:
We are currently working on a Web-based training program for shipment of
"biological materials". It will cover the basics (regs, principles etc.)
and guide the user through the process of getting the material ready and
out to the shipper.
The program will be up on a website soon and you don't need to buy a fancy
CD. It is based on FrontPage 98/2000 and allows full customization. As
soon as the beta-testing begins, I will post a follow-up note and will at
that time look for colleagues that would like to help me work the bugs out.
Thanks.
Stefan Wagener, Ph.D., CBSP
=========================================================================
Date: Mon, 3 May 1999 14:15:06 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: Re: Shipping Research Specimens
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
The proper shipping name would be "Diagnostic Specimen". You only need to
complete an Air Waybill, and not a Shipper's Declaration of Hazardous
Materials. This is also true if you are using dry ice to keep the samples
cold (refer to IATA packing instruction 904). However, there may be
additional packaging (outside of paperwork) requirements if the samples are
shipped on dry ice.
Julie A. Johnson, Ph.D.
Biosafety Officer
Iowa State University
Environmental Health & Safety
118 Agronomy Lab
phone: 515-294-7657
fax: 515-294-9357
E-mail: jajohns@iastate.edu
-----Original Message-----
From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
Sent: Monday, May 03, 1999 2:53 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: Shipping Research Specimens
Okay,
Now is there an appropriate Proper Shipping Name?
Bob
>Bob,
>Human blood and blood products that have no or very low probability of
>containing infectious disease agents do fall under the definition of
>"Diagnostic Specimens" as defined for the proper shipping name. UN
>certified packaging is not required, but they must be packed in accordance
>with IATA packaging instruction 650, which odes require that the packaging
>meet certain test criteria. The packing requirements are similar to those
>for "Infectious Substances", except for the lack of requirement for UN
>certified packaging.
>
>Julie A. Johnson, Ph.D.
>Biosafety Officer
>Iowa State University
>Environmental Health & Safety
>118 Agronomy Lab
>phone: 515-294-7657
>fax: 515-294-9357
>E-mail: jajohns@iastate.edu
>
>
>
>
>-----Original Message-----
>From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
>Sent: Monday, May 03, 1999 11:27 AM
>To: BIOSAFTY@MITVMA.MIT.EDU
>Subject: Re: Shipping Research Specimens
>
>
>I have just taken over our shipping program here. I am attempting to
>address several of the points you discuss and I have several more questions
>to raise.
>
>Training:
>
>My department has been training our researchers for years on how to ship
>specific items. I have descovered that nobody has kept records on who was
>trained.
>
>We now offer a sign-in sheet with our training which is filed in my
>department. Attached to this sheet is a synopsis of what the training was
>about. We do not train to ship everything. Instead we train for a
>specific item. This allow us to cookbook the training. The group gives me
>the names of the item(s) they intend to ship. I then research how to ship
>these items only. They are trained on that subject only. The synopsis
>includes packaging and all information from the CFR49 172.101 table. We
>cover shipping papers, shipping containers, labels, markings and inner
>containement for that item only. I also include research if the item is
>being exported and the material is TSCA regulated. Haven't had one of
>those yet.
>
>Specific material training can take as little as 30 minutes. Any time a
>different material is to be shipped, we must rain on the new material.
>
>Now, I have two questions that I am researching at this time.
>
>I have labs who are shipping human blood and blood products. These are not
>diagnostic speciments. They are research specimens. Diagnostic specimens
>is a Proper Shipping Name(PSN) that is exempt from several regulations.
>
>We do not test for infectious substances. Since we are handling blood,
>Universal Precautions and OSHA say to consider the material infectious.
>DOT says properly identify the hazard. DO NOT over classify.
>
>So should I ship human blood as "Infectious Substance, 6.2" or is it
>unregulated?
>
>Shipping containers:
>I am encountering several domestic shipping containers that do not have the
>proper markings to indicate that they are OK for hazardous materials.
>Instead they are being "Certified" for freight shipment with the
>specifications being listed. These containers are being provided to us
>from other labs, some very prestigeous, and we are being asked to ship our
>specimens in these containers. Are these containers acceptable?
>
>Bob
>
>
>>At 04:19 PM 4/30/99 -0400, you wrote:
>>>I just received a call from a researcher who wants to ship
>>>human blood and urine samples to another facility. She
>>>knows the proper procedures for packaging and labeling the
>>>specimens. However, she is very concerned because she has
>>>not attended an IATA approved course on Dangerous Goods
>>>Transportation.
>>
>>The DOT regs state that everyone who offers for shipment dangerous goods
>>must be trained. The type of training is not specified. In the short
term
>>she can probably work with the shipper to ensure that the material is
>>packaged correctly and the paper work is done correctly but she will need
>>to be trained.
>>
>>Richie Fink
>>M.I.T.
>>rfink@mit.edu
>
>
> ________________________________________________
>__ / _______________________________________________
>_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
>Safety
> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
> \ / U.S.A. RA Member Environmental
>Safety
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental
Safety
=========================================================================
Date: Mon, 3 May 1999 15:47:40 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Kimberly Sasur
Subject: In-House Blood Donation Programs
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
To the list,
I am researching the (NIH?) recommendations regarding an in-house blood
donation program, but have been unable to find any concrete information.
The blood will be used strictly for research. Does anyone participate in
such a program, or have any information regarding any requirements or
industry standards?
Thank you.
Kimberly A. Sasur
Safety Coordinator
CuraGen Corporation
555 Long Wharf Drive
New Haven, CT 06511
(203) 401-3330 Ext. 355
Fax (203) 401-3337
=========================================================================
Date: Mon, 3 May 1999 15:44:15 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: Shipping Research Specimens
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
I call blood and blood products where a relatively low probability exists
that pathogens of risk group 2 or 3 are present and when they are not being
shipped for the purpose of pathogenic diagnosis = BIOLOGICAL SPECIMENS or
BIOLOGICAL PRODUCTS. They are not declared as dangerous goods or
identified as infectious agents (Class 6.2) or packed under Packing
Instruction 602. But the DGR still regulates them and they should be
packed according to Packing Instruction 650 of the DGR. Judy Pointer
"Robert N. Latsch" on 05/03/99 02:52:54 PM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: Re: Shipping Research Specimens
Okay,
Now is there an appropriate Proper Shipping Name?
Bob
>Bob,
>Human blood and blood products that have no or very low probability of
>containing infectious disease agents do fall under the definition of
>"Diagnostic Specimens" as defined for the proper shipping name. UN
>certified packaging is not required, but they must be packed in accordance
>with IATA packaging instruction 650, which odes require that the packaging
>meet certain test criteria. The packing requirements are similar to those
>for "Infectious Substances", except for the lack of requirement for UN
>certified packaging.
>
>Julie A. Johnson, Ph.D.
>Biosafety Officer
>Iowa State University
>Environmental Health & Safety
>118 Agronomy Lab
>phone: 515-294-7657
>fax: 515-294-9357
>E-mail: jajohns@iastate.edu
>
>
>
>
>-----Original Message-----
>From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
>Sent: Monday, May 03, 1999 11:27 AM
>To: BIOSAFTY@MITVMA.MIT.EDU
>Subject: Re: Shipping Research Specimens
>
>
>I have just taken over our shipping program here. I am attempting to
>address several of the points you discuss and I have several more
questions
>to raise.
>
>Training:
>
>My department has been training our researchers for years on how to ship
>specific items. I have descovered that nobody has kept records on who was
>trained.
>
>We now offer a sign-in sheet with our training which is filed in my
>department. Attached to this sheet is a synopsis of what the training was
>about. We do not train to ship everything. Instead we train for a
>specific item. This allow us to cookbook the training. The group gives
me
>the names of the item(s) they intend to ship. I then research how to ship
>these items only. They are trained on that subject only. The synopsis
>includes packaging and all information from the CFR49 172.101 table. We
>cover shipping papers, shipping containers, labels, markings and inner
>containement for that item only. I also include research if the item is
>being exported and the material is TSCA regulated. Haven't had one of
>those yet.
>
>Specific material training can take as little as 30 minutes. Any time a
>different material is to be shipped, we must rain on the new material.
>
>Now, I have two questions that I am researching at this time.
>
>I have labs who are shipping human blood and blood products. These are
not
>diagnostic speciments. They are research specimens. Diagnostic specimens
>is a Proper Shipping Name(PSN) that is exempt from several regulations.
>
>We do not test for infectious substances. Since we are handling blood,
>Universal Precautions and OSHA say to consider the material infectious.
>DOT says properly identify the hazard. DO NOT over classify.
>
>So should I ship human blood as "Infectious Substance, 6.2" or is it
>unregulated?
>
>Shipping containers:
>I am encountering several domestic shipping containers that do not have
the
>proper markings to indicate that they are OK for hazardous materials.
>Instead they are being "Certified" for freight shipment with the
>specifications being listed. These containers are being provided to us
>from other labs, some very prestigeous, and we are being asked to ship our
>specimens in these containers. Are these containers acceptable?
>
>Bob
>
>
>>At 04:19 PM 4/30/99 -0400, you wrote:
>>>I just received a call from a researcher who wants to ship
>>>human blood and urine samples to another facility. She
>>>knows the proper procedures for packaging and labeling the
>>>specimens. However, she is very concerned because she has
>>>not attended an IATA approved course on Dangerous Goods
>>>Transportation.
>>
>>The DOT regs state that everyone who offers for shipment dangerous goods
>>must be trained. The type of training is not specified. In the short
term
>>she can probably work with the shipper to ensure that the material is
>>packaged correctly and the paper work is done correctly but she will need
>>to be trained.
>>
>>Richie Fink
>>M.I.T.
>>rfink@mit.edu
>
>
> ________________________________________________
>__ / _______________________________________________
>_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
>Safety
> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
> \ / U.S.A. RA Member Environmental
>Safety
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental
Safety
=========================================================================
Date: Mon, 3 May 1999 17:13:32 -0400
Reply-To: johnson@
Sender: A Biosafety Discussion List
From: Jay Johnson
Subject: Re: Shipping Research Specimens
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Judy
According to 49 CFR..
173.134(a)(3) a Biological product means a material that is prepared and
manufactured in accordance with the provisions of 9 CFR part 102 (Licenses
for biological products), 9 CFR part 103 (Experimental products,
distribution, and evaluation of biological products prior to licensing), 9
CFR part 104 (Permits for biological products), 21 CFR part 312
(Investigational new drug application), or 21 CFR parts 600 to 680
(Biologics).
I think the IATA definition of Diagnostic Specimens (3.6.2.1.4) more aptly
applies in your situation instead of Biological Products. Also, Biological
Products are no longer required to be shipped according to Packing
Instruction 650 of the DGR.
I will be happy to answer any questions you may have. I can be reached at
1-800-497-6973.
Jay Johnson
Partners In Compliance
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU] On Behalf
Of Judy Pointer
Sent: Monday, May 03, 1999 4:44 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: Shipping Research Specimens
I call blood and blood products where a relatively low probability exists
that pathogens of risk group 2 or 3 are present and when they are not being
shipped for the purpose of pathogenic diagnosis = BIOLOGICAL SPECIMENS or
BIOLOGICAL PRODUCTS. They are not declared as dangerous goods or
identified as infectious agents (Class 6.2) or packed under Packing
Instruction 602. But the DGR still regulates them and they should be
packed according to Packing Instruction 650 of the DGR. Judy Pointer
"Robert N. Latsch" on 05/03/99 02:52:54 PM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: Re: Shipping Research Specimens
Okay,
Now is there an appropriate Proper Shipping Name?
Bob
>Bob,
>Human blood and blood products that have no or very low probability of
>containing infectious disease agents do fall under the definition of
>"Diagnostic Specimens" as defined for the proper shipping name. UN
>certified packaging is not required, but they must be packed in accordance
>with IATA packaging instruction 650, which odes require that the packaging
>meet certain test criteria. The packing requirements are similar to those
>for "Infectious Substances", except for the lack of requirement for UN
>certified packaging.
>
>Julie A. Johnson, Ph.D.
>Biosafety Officer
>Iowa State University
>Environmental Health & Safety
>118 Agronomy Lab
>phone: 515-294-7657
>fax: 515-294-9357
>E-mail: jajohns@iastate.edu
>
>
>
>
>-----Original Message-----
>From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
>Sent: Monday, May 03, 1999 11:27 AM
>To: BIOSAFTY@MITVMA.MIT.EDU
>Subject: Re: Shipping Research Specimens
>
>
>I have just taken over our shipping program here. I am attempting to
>address several of the points you discuss and I have several more
questions
>to raise.
>
>Training:
>
>My department has been training our researchers for years on how to ship
>specific items. I have descovered that nobody has kept records on who was
>trained.
>
>We now offer a sign-in sheet with our training which is filed in my
>department. Attached to this sheet is a synopsis of what the training was
>about. We do not train to ship everything. Instead we train for a
>specific item. This allow us to cookbook the training. The group gives
me
>the names of the item(s) they intend to ship. I then research how to ship
>these items only. They are trained on that subject only. The synopsis
>includes packaging and all information from the CFR49 172.101 table. We
>cover shipping papers, shipping containers, labels, markings and inner
>containement for that item only. I also include research if the item is
>being exported and the material is TSCA regulated. Haven't had one of
>those yet.
>
>Specific material training can take as little as 30 minutes. Any time a
>different material is to be shipped, we must rain on the new material.
>
>Now, I have two questions that I am researching at this time.
>
>I have labs who are shipping human blood and blood products. These are
not
>diagnostic speciments. They are research specimens. Diagnostic specimens
>is a Proper Shipping Name(PSN) that is exempt from several regulations.
>
>We do not test for infectious substances. Since we are handling blood,
>Universal Precautions and OSHA say to consider the material infectious.
>DOT says properly identify the hazard. DO NOT over classify.
>
>So should I ship human blood as "Infectious Substance, 6.2" or is it
>unregulated?
>
>Shipping containers:
>I am encountering several domestic shipping containers that do not have
the
>proper markings to indicate that they are OK for hazardous materials.
>Instead they are being "Certified" for freight shipment with the
>specifications being listed. These containers are being provided to us
>from other labs, some very prestigeous, and we are being asked to ship our
>specimens in these containers. Are these containers acceptable?
>
>Bob
>
>
>>At 04:19 PM 4/30/99 -0400, you wrote:
>>>I just received a call from a researcher who wants to ship
>>>human blood and urine samples to another facility. She
>>>knows the proper procedures for packaging and labeling the
>>>specimens. However, she is very concerned because she has
>>>not attended an IATA approved course on Dangerous Goods
>>>Transportation.
>>
>>The DOT regs state that everyone who offers for shipment dangerous goods
>>must be trained. The type of training is not specified. In the short
term
>>she can probably work with the shipper to ensure that the material is
>>packaged correctly and the paper work is done correctly but she will need
>>to be trained.
>>
>>Richie Fink
>>M.I.T.
>>rfink@mit.edu
>
>
> ________________________________________________
>__ / _______________________________________________
>_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
>Safety
> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
> \ / U.S.A. RA Member Environmental
>Safety
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental
Safety
=========================================================================
Date: Mon, 3 May 1999 17:14:40 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Shipping Research Specimens
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Julie,
My problem is that these are not diagnostic specimens. They are research
specimens. Diagnostic specimens do have a definition. Which means to be
used in the diagnosis or treatment of an individual. DOT does have
penalties for misrepresenting materials:)
bob
>The proper shipping name would be "Diagnostic Specimen". You only need to
>complete an Air Waybill, and not a Shipper's Declaration of Hazardous
>Materials. This is also true if you are using dry ice to keep the samples
>cold (refer to IATA packing instruction 904). However, there may be
>additional packaging (outside of paperwork) requirements if the samples are
>shipped on dry ice.
>
>Julie A. Johnson, Ph.D.
>Biosafety Officer
>Iowa State University
>Environmental Health & Safety
>118 Agronomy Lab
>phone: 515-294-7657
>fax: 515-294-9357
>E-mail: jajohns@iastate.edu
>
>
>
>
>-----Original Message-----
>From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
>Sent: Monday, May 03, 1999 2:53 PM
>To: BIOSAFTY@MITVMA.MIT.EDU
>Subject: Re: Shipping Research Specimens
>
>
>Okay,
>
>Now is there an appropriate Proper Shipping Name?
>
>Bob
>
>>Bob,
>>Human blood and blood products that have no or very low probability of
>>containing infectious disease agents do fall under the definition of
>>"Diagnostic Specimens" as defined for the proper shipping name. UN
>>certified packaging is not required, but they must be packed in accordance
>>with IATA packaging instruction 650, which odes require that the packaging
>>meet certain test criteria. The packing requirements are similar to those
>>for "Infectious Substances", except for the lack of requirement for UN
>>certified packaging.
>>
>>Julie A. Johnson, Ph.D.
>>Biosafety Officer
>>Iowa State University
>>Environmental Health & Safety
>>118 Agronomy Lab
>>phone: 515-294-7657
>>fax: 515-294-9357
>>E-mail: jajohns@iastate.edu
>>
>>
>>
>>
>>-----Original Message-----
>>From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
>>Sent: Monday, May 03, 1999 11:27 AM
>>To: BIOSAFTY@MITVMA.MIT.EDU
>>Subject: Re: Shipping Research Specimens
>>
>>
>>I have just taken over our shipping program here. I am attempting to
>>address several of the points you discuss and I have several more questions
>>to raise.
>>
>>Training:
>>
>>My department has been training our researchers for years on how to ship
>>specific items. I have descovered that nobody has kept records on who was
>>trained.
>>
>>We now offer a sign-in sheet with our training which is filed in my
>>department. Attached to this sheet is a synopsis of what the training was
>>about. We do not train to ship everything. Instead we train for a
>>specific item. This allow us to cookbook the training. The group gives me
>>the names of the item(s) they intend to ship. I then research how to ship
>>these items only. They are trained on that subject only. The synopsis
>>includes packaging and all information from the CFR49 172.101 table. We
>>cover shipping papers, shipping containers, labels, markings and inner
>>containement for that item only. I also include research if the item is
>>being exported and the material is TSCA regulated. Haven't had one of
>>those yet.
>>
>>Specific material training can take as little as 30 minutes. Any time a
>>different material is to be shipped, we must rain on the new material.
>>
>>Now, I have two questions that I am researching at this time.
>>
>>I have labs who are shipping human blood and blood products. These are not
>>diagnostic speciments. They are research specimens. Diagnostic specimens
>>is a Proper Shipping Name(PSN) that is exempt from several regulations.
>>
>>We do not test for infectious substances. Since we are handling blood,
>>Universal Precautions and OSHA say to consider the material infectious.
>>DOT says properly identify the hazard. DO NOT over classify.
>>
>>So should I ship human blood as "Infectious Substance, 6.2" or is it
>>unregulated?
>>
>>Shipping containers:
>>I am encountering several domestic shipping containers that do not have the
>>proper markings to indicate that they are OK for hazardous materials.
>>Instead they are being "Certified" for freight shipment with the
>>specifications being listed. These containers are being provided to us
>>from other labs, some very prestigeous, and we are being asked to ship our
>>specimens in these containers. Are these containers acceptable?
>>
>>Bob
>>
>>
>>>At 04:19 PM 4/30/99 -0400, you wrote:
>>>>I just received a call from a researcher who wants to ship
>>>>human blood and urine samples to another facility. She
>>>>knows the proper procedures for packaging and labeling the
>>>>specimens. However, she is very concerned because she has
>>>>not attended an IATA approved course on Dangerous Goods
>>>>Transportation.
>>>
>>>The DOT regs state that everyone who offers for shipment dangerous goods
>>>must be trained. The type of training is not specified. In the short
>term
>>>she can probably work with the shipper to ensure that the material is
>>>packaged correctly and the paper work is done correctly but she will need
>>>to be trained.
>>>
>>>Richie Fink
>>>M.I.T.
>>>rfink@mit.edu
>>
>>
>> ________________________________________________
>>__ / _______________________________________________
>>_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
>>Safety
>> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
>> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
>> \ / U.S.A. RA Member Environmental
>>Safety
>
>
> ________________________________________________
>__ / _______________________________________________
>_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
>Safety
> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
> \ / U.S.A. RA Member Environmental
>Safety
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Mon, 3 May 1999 17:37:07 -0400
Reply-To: johnson@
Sender: A Biosafety Discussion List
From: Jay Johnson
Subject: Re: Shipping Research Specimens
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Bob
The definition of Diagnostic Specimens under IATA 3.6.2.1.4 now covers "any
human or animal material including, but not limited to, excreta, secreta,
blood and its components, tissues and tissue fluids, being transported for
diagnostic or investigational purposes, but excluding live infected
animals."
research specimens = investigational specimens =diagnostic specimens
Jay Johnson
Partners In Compliance
1-800-497-6973
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU] On Behalf
Of Robert N. Latsch
Sent: Monday, May 03, 1999 6:15 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: Shipping Research Specimens
Julie,
My problem is that these are not diagnostic specimens. They are research
specimens. Diagnostic specimens do have a definition. Which means to be
used in the diagnosis or treatment of an individual. DOT does have
penalties for misrepresenting materials:)
bob
>The proper shipping name would be "Diagnostic Specimen". You only need to
>complete an Air Waybill, and not a Shipper's Declaration of Hazardous
>Materials. This is also true if you are using dry ice to keep the samples
>cold (refer to IATA packing instruction 904). However, there may be
>additional packaging (outside of paperwork) requirements if the samples are
>shipped on dry ice.
>
>Julie A. Johnson, Ph.D.
>Biosafety Officer
>Iowa State University
>Environmental Health & Safety
>118 Agronomy Lab
>phone: 515-294-7657
>fax: 515-294-9357
>E-mail: jajohns@iastate.edu
>
>
>
>
>-----Original Message-----
>From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
>Sent: Monday, May 03, 1999 2:53 PM
>To: BIOSAFTY@MITVMA.MIT.EDU
>Subject: Re: Shipping Research Specimens
>
>
>Okay,
>
>Now is there an appropriate Proper Shipping Name?
>
>Bob
>
>>Bob,
>>Human blood and blood products that have no or very low probability of
>>containing infectious disease agents do fall under the definition of
>>"Diagnostic Specimens" as defined for the proper shipping name. UN
>>certified packaging is not required, but they must be packed in accordance
>>with IATA packaging instruction 650, which odes require that the packaging
>>meet certain test criteria. The packing requirements are similar to those
>>for "Infectious Substances", except for the lack of requirement for UN
>>certified packaging.
>>
>>Julie A. Johnson, Ph.D.
>>Biosafety Officer
>>Iowa State University
>>Environmental Health & Safety
>>118 Agronomy Lab
>>phone: 515-294-7657
>>fax: 515-294-9357
>>E-mail: jajohns@iastate.edu
>>
>>
>>
>>
>>-----Original Message-----
>>From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
>>Sent: Monday, May 03, 1999 11:27 AM
>>To: BIOSAFTY@MITVMA.MIT.EDU
>>Subject: Re: Shipping Research Specimens
>>
>>
>>I have just taken over our shipping program here. I am attempting to
>>address several of the points you discuss and I have several more
questions
>>to raise.
>>
>>Training:
>>
>>My department has been training our researchers for years on how to ship
>>specific items. I have descovered that nobody has kept records on who was
>>trained.
>>
>>We now offer a sign-in sheet with our training which is filed in my
>>department. Attached to this sheet is a synopsis of what the training was
>>about. We do not train to ship everything. Instead we train for a
>>specific item. This allow us to cookbook the training. The group gives
me
>>the names of the item(s) they intend to ship. I then research how to ship
>>these items only. They are trained on that subject only. The synopsis
>>includes packaging and all information from the CFR49 172.101 table. We
>>cover shipping papers, shipping containers, labels, markings and inner
>>containement for that item only. I also include research if the item is
>>being exported and the material is TSCA regulated. Haven't had one of
>>those yet.
>>
>>Specific material training can take as little as 30 minutes. Any time a
>>different material is to be shipped, we must rain on the new material.
>>
>>Now, I have two questions that I am researching at this time.
>>
>>I have labs who are shipping human blood and blood products. These are
not
>>diagnostic speciments. They are research specimens. Diagnostic specimens
>>is a Proper Shipping Name(PSN) that is exempt from several regulations.
>>
>>We do not test for infectious substances. Since we are handling blood,
>>Universal Precautions and OSHA say to consider the material infectious.
>>DOT says properly identify the hazard. DO NOT over classify.
>>
>>So should I ship human blood as "Infectious Substance, 6.2" or is it
>>unregulated?
>>
>>Shipping containers:
>>I am encountering several domestic shipping containers that do not have
the
>>proper markings to indicate that they are OK for hazardous materials.
>>Instead they are being "Certified" for freight shipment with the
>>specifications being listed. These containers are being provided to us
>>from other labs, some very prestigeous, and we are being asked to ship our
>>specimens in these containers. Are these containers acceptable?
>>
>>Bob
>>
>>
>>>At 04:19 PM 4/30/99 -0400, you wrote:
>>>>I just received a call from a researcher who wants to ship
>>>>human blood and urine samples to another facility. She
>>>>knows the proper procedures for packaging and labeling the
>>>>specimens. However, she is very concerned because she has
>>>>not attended an IATA approved course on Dangerous Goods
>>>>Transportation.
>>>
>>>The DOT regs state that everyone who offers for shipment dangerous goods
>>>must be trained. The type of training is not specified. In the short
>term
>>>she can probably work with the shipper to ensure that the material is
>>>packaged correctly and the paper work is done correctly but she will need
>>>to be trained.
>>>
>>>Richie Fink
>>>M.I.T.
>>>rfink@mit.edu
>>
>>
>> ________________________________________________
>>__ / _______________________________________________
>>_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
>>Safety
>> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
>> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
>> \ / U.S.A. RA Member Environmental
>>Safety
>
>
> ________________________________________________
>__ / _______________________________________________
>_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
>Safety
> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
> \ / U.S.A. RA Member Environmental
>Safety
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental
Safety
=========================================================================
Date: Mon, 3 May 1999 16:52:20 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: Re: Shipping Research Specimens
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
They are not diagnostic specimens according to how we would logically
classify them. Regulatory definitions can be somewhat different; read these
and see how they apply to your particular shipment.
According to the IATA Dangerous Goods Regulations, Section 3.6.2.1.4,
diagnostic specimens are "Any human or animal material including, but not
limited to, excreta, secreta, blood and its components, tissue and tissue
fluids, being transported for diagnostic or investigational purposes, but
excluding live infected animals."
According to Section 3.6.2.1.1, infectious substances "are substances known
to contain, or reasonably expected to contain, pathogens...."
According to Section 3.6.2.1.3, biological products are "those products
derived from living organisms, that are manufactured and distributed in
accordance with the requirements of the national governmental authorities
which may have special licensing requirements, and are used for prevention,
treatment, diagnosis of disease in humans or animals, or for development,
experimental or investigational purposes related thereto. They include, but
are not limited to, finished or unfinished products such as vaccines and
diagnostic products."
I hope this helps to clarify what can be a very murky subject.
Julie A. Johnson, Ph.D.
Biosafety Officer
Iowa State University
Environmental Health & Safety
118 Agronomy Lab
phone: 515-294-7657
fax: 515-294-9357
E-mail: jajohns@iastate.edu
-----Original Message-----
From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
Sent: Monday, May 03, 1999 5:15 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: Shipping Research Specimens
Julie,
My problem is that these are not diagnostic specimens. They are research
specimens. Diagnostic specimens do have a definition. Which means to be
used in the diagnosis or treatment of an individual. DOT does have
penalties for misrepresenting materials:)
bob
>The proper shipping name would be "Diagnostic Specimen". You only need to
>complete an Air Waybill, and not a Shipper's Declaration of Hazardous
>Materials. This is also true if you are using dry ice to keep the samples
>cold (refer to IATA packing instruction 904). However, there may be
>additional packaging (outside of paperwork) requirements if the samples are
>shipped on dry ice.
>
>Julie A. Johnson, Ph.D.
>Biosafety Officer
>Iowa State University
>Environmental Health & Safety
>118 Agronomy Lab
>phone: 515-294-7657
>fax: 515-294-9357
>E-mail: jajohns@iastate.edu
>
>
>
>
>-----Original Message-----
>From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
>Sent: Monday, May 03, 1999 2:53 PM
>To: BIOSAFTY@MITVMA.MIT.EDU
>Subject: Re: Shipping Research Specimens
>
>
>Okay,
>
>Now is there an appropriate Proper Shipping Name?
>
>Bob
>
>>Bob,
>>Human blood and blood products that have no or very low probability of
>>containing infectious disease agents do fall under the definition of
>>"Diagnostic Specimens" as defined for the proper shipping name. UN
>>certified packaging is not required, but they must be packed in accordance
>>with IATA packaging instruction 650, which odes require that the packaging
>>meet certain test criteria. The packing requirements are similar to those
>>for "Infectious Substances", except for the lack of requirement for UN
>>certified packaging.
>>
>>Julie A. Johnson, Ph.D.
>>Biosafety Officer
>>Iowa State University
>>Environmental Health & Safety
>>118 Agronomy Lab
>>phone: 515-294-7657
>>fax: 515-294-9357
>>E-mail: jajohns@iastate.edu
>>
>>
>>
>>
>>-----Original Message-----
>>From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
>>Sent: Monday, May 03, 1999 11:27 AM
>>To: BIOSAFTY@MITVMA.MIT.EDU
>>Subject: Re: Shipping Research Specimens
>>
>>
>>I have just taken over our shipping program here. I am attempting to
>>address several of the points you discuss and I have several more
questions
>>to raise.
>>
>>Training:
>>
>>My department has been training our researchers for years on how to ship
>>specific items. I have descovered that nobody has kept records on who was
>>trained.
>>
>>We now offer a sign-in sheet with our training which is filed in my
>>department. Attached to this sheet is a synopsis of what the training was
>>about. We do not train to ship everything. Instead we train for a
>>specific item. This allow us to cookbook the training. The group gives
me
>>the names of the item(s) they intend to ship. I then research how to ship
>>these items only. They are trained on that subject only. The synopsis
>>includes packaging and all information from the CFR49 172.101 table. We
>>cover shipping papers, shipping containers, labels, markings and inner
>>containement for that item only. I also include research if the item is
>>being exported and the material is TSCA regulated. Haven't had one of
>>those yet.
>>
>>Specific material training can take as little as 30 minutes. Any time a
>>different material is to be shipped, we must rain on the new material.
>>
>>Now, I have two questions that I am researching at this time.
>>
>>I have labs who are shipping human blood and blood products. These are
not
>>diagnostic speciments. They are research specimens. Diagnostic specimens
>>is a Proper Shipping Name(PSN) that is exempt from several regulations.
>>
>>We do not test for infectious substances. Since we are handling blood,
>>Universal Precautions and OSHA say to consider the material infectious.
>>DOT says properly identify the hazard. DO NOT over classify.
>>
>>So should I ship human blood as "Infectious Substance, 6.2" or is it
>>unregulated?
>>
>>Shipping containers:
>>I am encountering several domestic shipping containers that do not have
the
>>proper markings to indicate that they are OK for hazardous materials.
>>Instead they are being "Certified" for freight shipment with the
>>specifications being listed. These containers are being provided to us
>>from other labs, some very prestigeous, and we are being asked to ship our
>>specimens in these containers. Are these containers acceptable?
>>
>>Bob
>>
>>
>>>At 04:19 PM 4/30/99 -0400, you wrote:
>>>>I just received a call from a researcher who wants to ship
>>>>human blood and urine samples to another facility. She
>>>>knows the proper procedures for packaging and labeling the
>>>>specimens. However, she is very concerned because she has
>>>>not attended an IATA approved course on Dangerous Goods
>>>>Transportation.
>>>
>>>The DOT regs state that everyone who offers for shipment dangerous goods
>>>must be trained. The type of training is not specified. In the short
>term
>>>she can probably work with the shipper to ensure that the material is
>>>packaged correctly and the paper work is done correctly but she will need
>>>to be trained.
>>>
>>>Richie Fink
>>>M.I.T.
>>>rfink@mit.edu
>>
>>
>> ________________________________________________
>>__ / _______________________________________________
>>_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
>>Safety
>> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
>> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
>> \ / U.S.A. RA Member Environmental
>>Safety
>
>
> ________________________________________________
>__ / _______________________________________________
>_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
>Safety
> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
> \ / U.S.A. RA Member Environmental
>Safety
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental
Safety
=========================================================================
Date: Mon, 3 May 1999 17:04:11 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Clifford W. Bond"
Subject: Re: Shipping & Training on the Web!
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Stefan,
I would be delighted to help you debug your shipping regs training site!
Cliff Bond
Clifford W. Bond, Professor
Department of Microbiology
Montana State University
Bozeman, MT 59717-3520
Email: umbcb@gemini.oscs.montana.edu
Internet:
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Stefan Wagener
Sent: Monday, May 03, 1999 1:06 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Shipping & Training on the Web!
Since this training topic (Shipping of infectious agents) is of
considerable interest:
We are currently working on a Web-based training program for shipment of
"biological materials". It will cover the basics (regs, principles etc.)
and guide the user through the process of getting the material ready and
out to the shipper.
The program will be up on a website soon and you don't need to buy a fancy
CD. It is based on FrontPage 98/2000 and allows full customization. As
soon as the beta-testing begins, I will post a follow-up note and will at
that time look for colleagues that would like to help me work the bugs out.
Thanks.
Stefan Wagener, Ph.D., CBSP
=========================================================================
Date: Tue, 4 May 1999 09:38:32 +1000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Le Blanc Smith, Peter"
Subject: Re: Shipping Research Specimens
MIME-Version: 1.0
Content-Type: multipart/alternative;
boundary="----_=_NextPart_001_01BE95BE.0DFAE100"
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this format, some or all of this message may not be legible.
------_=_NextPart_001_01BE95BE.0DFAE100
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It is good news that the researcher has a proactive and responsible view of
dangerous goods transport. I don't think that there is enough information
to allow anyone to provide comprehensive advice.
Although the researcher has told you that they know the proper procedures
for packaging and labeling the
specimens, they may not be aware of recent changes affecting definition and
therefore packaging restrictions or lack of restrictions for diagnostic
samples. Are these diagnostic samples? Do they have hazards, biological or
other? IATA was mentioned, are the specimens going by air?
There have been significant changes to the IATA Dangerous Goods Regulations,
in force since January 1999. For example, changes to the definition of
diagnostic specimen.
It may be prudent to obtain advice from a shipper of dangerous goods, obtain
proper training or turn over responsibility for shipping to a qualified
shipper who meets the training responsibilities outlined in the Regulations.
Peter Le Blanc Smith
Biocontainment Microbiologist
CSIRO Animal Health
Australian Animal Health Laboratory
Private Mail Bag 24
Geelong Vic 3220
Australia
Ph: (03) 5227 5451 (including voice mail)
Int: +61 3 5227 5451
Fax: (03) 5227 5555
Int: +61 3 5227 5555
E-mail address. Peter.Le.Blanc.Smith@dah.csiro.au
-----Original Message-----
From: Jean.Goldberg [SMTP:Jean.Goldberg@MED.NYU.EDU]
Sent: Saturday, May 01, 1999 6:19 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Shipping Research Specimens
I just received a call from a researcher who wants to ship
human blood and urine samples to another facility. She
knows the proper procedures for packaging and labeling the
specimens. However, she is very concerned because she has
not attended an IATA approved course on Dangerous Goods
Transportation. She is worried about being fined $10,000.
Question: Does she really need to attend an IATA approved
training course in order to be in compliance with the
regulations. Thanks in advance for any help you can
provide.
----------------------------------------
Jean Goldberg
Email: Jean.Goldberg@Med.Nyu.Edu
"NYU Medical Center"
------_=_NextPart_001_01BE95BE.0DFAE100
Content-Type: text/html
Content-Transfer-Encoding: quoted-printable
RE: Shipping Research Specimens
It is good news that the researcher =
has a proactive and responsible view of dangerous goods =
transport. I don't think that there is enough information to =
allow anyone to provide comprehensive advice.
Although the researcher has told you =
that they know the proper =
procedures for packaging and labeling the
specimens, they may not be aware of recent changes affecting definition =
and therefore packaging restrictions or lack of restrictions for =
diagnostic samples. Are these diagnostic samples? Do they have hazards, =
biological or other? IATA was mentioned, are the specimens going by =
air?
There have been significant changes to =
the IATA Dangerous Goods Regulations, in force since January 1999. For =
example, changes to the definition of =
diagnostic specimen.
It may be prudent to obtain advice =
from a shipper of dangerous goods, obtain proper training or turn over =
responsibility for shipping to a qualified shipper who meets the =
training responsibilities outlined in the Regulations.
Peter Le Blanc =
Smith
Biocontainment =
Microbiologist
CSIRO Animal Health
Australian Animal Health =
Laboratory
Private Mail Bag 24
Geelong =
Vic 3220
Australia
Ph: (03) 5227 5451 =
(including voice mail)
Int: +61 3 5227 5451
Fax: (03) 5227 =
5555
Int: +61 3 5227 5555
E-mail address. Peter.Le.Blanc.Smith@dah.csiro.au
-----Original =
Message-----
From: Jean.Goldberg =
[SMTP:Jean.Goldberg@MED.NYU.EDU]
Sent: Saturday, May 01, 1999 6:19 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: =
Shipping Research Specimens
I just received a call from a =
researcher who wants to ship
human blood and urine samples to =
another facility. She
knows the proper procedures for =
packaging and labeling the
specimens. However, she is very =
concerned because she has
not attended an IATA approved course =
on Dangerous Goods
Transportation. She is worried =
about being fined $10,000.
Question: Does she really need to =
attend an IATA approved
training course in order to be in =
compliance with the
regulations. Thanks in advance =
for any help you can
provide.
----------------------------------------
Jean Goldberg
Email: =
Jean.Goldberg@Med.Nyu.Edu
"NYU Medical Center"
------_=_NextPart_001_01BE95BE.0DFAE100--
=========================================================================
Date: Tue, 4 May 1999 09:55:46 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jairo Betancourt
Subject: Re: Shipping Research Specimens
MIME-version: 1.0
Content-type: multipart/alternative;
boundary="----=_NextPart_000_0019_01BE9614.4831FB20"
This is a multi-part message in MIME format.
------=_NextPart_000_0019_01BE9614.4831FB20
Content-Type: text/plain;
charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
This is why we need the training- to play the game by the rules. =
Technical or scientific names are different to Proper Shipping Names. In =
the case of a regular blood sample for routine diagnostic it is called =
Diagnostic specimen, but is is not a Proper Shipping Name. Once it is =
not infectious, then is out of the regs for compliance, except perhaps =
for the packaging. I am quoting:
" Diagnostic specimens known not to contain pathogens in Risk Groups 2, =
3, or 4-----------Not Restricted"
" Diagnostic specimens known or reasonable expected to contain pathogens =
in Risk Groups 2, 3, or 4 and those where =20
a relatively low probability exists that pathogens of risk group 4 =
are present, see Infectious Substance, etc. (UN 2814,=20
UN2900)"
"Diagnostic specimens with a low probability of containing pathogens in =
Risk Groups 2 or 3"-----Packing instructions 650-
-max. amount 4L, etc. =20
In many cases this may come down to your judgment call.
-----Original Message-----
From: Robert N. Latsch
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Monday, May 03, 1999 2:54 PM
Subject: Re: Shipping Research Specimens
>Okay,
>
>Now is there an appropriate Proper Shipping Name?
>
>Bob
>
>>Bob,
>>Human blood and blood products that have no or very low probability of
>>containing infectious disease agents do fall under the definition of
>>"Diagnostic Specimens" as defined for the proper shipping name. UN
>>certified packaging is not required, but they must be packed in =
accordance
>>with IATA packaging instruction 650, which odes require that the =
packaging
>>meet certain test criteria. The packing requirements are similar to =
those
>>for "Infectious Substances", except for the lack of requirement for UN
>>certified packaging.
>>
>>Julie A. Johnson, Ph.D.
>>Biosafety Officer
>>Iowa State University
>>Environmental Health & Safety
>>118 Agronomy Lab
>>phone: 515-294-7657
>>fax: 515-294-9357
>>E-mail: jajohns@iastate.edu
>>
>>
>>
>>
>>-----Original Message-----
>>From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]
>>Sent: Monday, May 03, 1999 11:27 AM
>>To: BIOSAFTY@MITVMA.MIT.EDU
>>Subject: Re: Shipping Research Specimens
>>
>>
>>I have just taken over our shipping program here. I am attempting to
>>address several of the points you discuss and I have several more =
questions
>>to raise.
>>
>>Training:
>>
>>My department has been training our researchers for years on how to =
ship
>>specific items. I have descovered that nobody has kept records on who =
was
>>trained.
>>
>>We now offer a sign-in sheet with our training which is filed in my
>>department. Attached to this sheet is a synopsis of what the training =
was
>>about. We do not train to ship everything. Instead we train for a
>>specific item. This allow us to cookbook the training. The group =
gives me
>>the names of the item(s) they intend to ship. I then research how to =
ship
>>these items only. They are trained on that subject only. The synopsis
>>includes packaging and all information from the CFR49 172.101 table. =
We
>>cover shipping papers, shipping containers, labels, markings and inner
>>containement for that item only. I also include research if the item =
is
>>being exported and the material is TSCA regulated. Haven't had one of
>>those yet.
>>
>>Specific material training can take as little as 30 minutes. Any time =
a
>>different material is to be shipped, we must rain on the new material.
>>
>>Now, I have two questions that I am researching at this time.
>>
>>I have labs who are shipping human blood and blood products. These =
are not
>>diagnostic speciments. They are research specimens. Diagnostic =
specimens
>>is a Proper Shipping Name(PSN) that is exempt from several =
regulations.
>>
>>We do not test for infectious substances. Since we are handling =
blood,
>>Universal Precautions and OSHA say to consider the material =
infectious.
>>DOT says properly identify the hazard. DO NOT over classify.
>>
>>So should I ship human blood as "Infectious Substance, 6.2" or is it
>>unregulated?
>>
>>Shipping containers:
>>I am encountering several domestic shipping containers that do not =
have the
>>proper markings to indicate that they are OK for hazardous materials.
>>Instead they are being "Certified" for freight shipment with the
>>specifications being listed. These containers are being provided to =
us
>>from other labs, some very prestigeous, and we are being asked to ship =
our
>>specimens in these containers. Are these containers acceptable?
>>
>>Bob
>>
>>
>>>At 04:19 PM 4/30/99 -0400, you wrote:
>>>>I just received a call from a researcher who wants to ship
>>>>human blood and urine samples to another facility. She
>>>>knows the proper procedures for packaging and labeling the
>>>>specimens. However, she is very concerned because she has
>>>>not attended an IATA approved course on Dangerous Goods
>>>>Transportation.
>>>
>>>The DOT regs state that everyone who offers for shipment dangerous =
goods
>>>must be trained. The type of training is not specified. In the =
short term
>>>she can probably work with the shipper to ensure that the material is
>>>packaged correctly and the paper work is done correctly but she will =
need
>>>to be trained.
>>>
>>>Richie Fink
>>>M.I.T.
>>>rfink@mit.edu
>>
>>
>> ________________________________________________
>>__ / _______________________________________________
>>_ \ / /Robert N. Latsch USSF State Referee 6 Environmental
>>Safety
>> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
>> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational =
&
>> \ / U.S.A. RA Member Environmental
>>Safety
>
>
> ________________________________________________
>__ / _______________________________________________
>_ \ / /Robert N. Latsch USSF State Referee 6 Environmental =
Safety
> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
> \ / U.S.A. RA Member Environmental =
Safety
------=_NextPart_000_0019_01BE9614.4831FB20
Content-Type: text/html;
charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
This is why we need the training- to play the game by the rules. =
Technical=20
or scientific names are different to Proper Shipping =
Names. In=20
the case of a regular blood sample for routine diagnostic it is called=20
Diagnostic specimen, but is is not a Proper Shipping =
Name. Once=20
it is not infectious, then is out of the regs for compliance, except =
perhaps for=20
the packaging. I am quoting:
" Diagnostic specimens known not to contain pathogens in Risk =
Groups=20
2, 3, or 4-----------Not Restricted"
" Diagnostic specimens known or reasonable expected to contain =
pathogens in Risk Groups 2, 3, or 4 and those =
where =20
a relatively low probability exists that pathogens of =
risk=20
group 4 are present, see Infectious Substance, etc. (UN =
2814,
UN2900)"
"Diagnostic specimens with a low probability of containing =
pathogens=20
in Risk Groups 2 or 3"-----Packing instructions 650-
-max. amount 4L, etc.
In many cases this may come down to your judgment call.
-----Original Message-----From: =
Robert N.=20
Latsch <rnl2@PO.CWRU.EDU>To: BIOSAFTY@MITVMA.MIT.EDU =
<BIOSAFTY@MITVMA.MIT.EDU>Date:=20
Monday, May 03, 1999 2:54 PMSubject: Re: Shipping Research=20
Specimens>Okay,>>Now is there an=20
appropriate Proper Shipping=20
Name?>>Bob>>>Bob,>>Human blood =
and=20
blood products that have no or very low probability =
of>>containing=20
infectious disease agents do fall under the definition=20
of>>"Diagnostic Specimens" as defined for the proper =
shipping name. UN>>certified packaging is not required, =
but they=20
must be packed in accordance>>with IATA packaging instruction =
650,=20
which odes require that the packaging>>meet certain test=20
criteria. The packing requirements are similar to =
those>>for=20
"Infectious Substances", except for the lack of requirement =
for=20
UN>>certified packaging.>>>>Julie A. =
Johnson,=20
Ph.D.>>Biosafety Officer>>Iowa State=20
University>>Environmental Health & Safety>>118 =
Agronomy=20
Lab>>phone: 515-294-7657>>fax:=20
515-294-9357>>E-mail: jajohns@iastate.edu>>
;>>>>>>>-----Original=20
Message----->>From: Robert N. Latsch [mailto:rnl2@PO.CWRU.EDU]>>=
Sent:=20
Monday, May 03, 1999 11:27 AM>>To: BIOSAFTY@MITVMA.MIT.EDU&g=
t;>Subject:=20
Re: Shipping Research Specimens>>>>>>I =
have just=20
taken over our shipping program here. I am attempting =
to>>address=20
several of the points you discuss and I have several more=20
questions>>to=20
raise.>>>>Training:>>>>My =
department has=20
been training our researchers for years on how to =
ship>>specific=20
items. I have descovered that nobody has kept records on who=20
was>>trained.>>>>We now offer a sign-in =
sheet with=20
our training which is filed in my>>department. Attached =
to this=20
sheet is a synopsis of what the training was>>about. We =
do not=20
train to ship everything. Instead we train for =
a>>specific=20
item. This allow us to cookbook the training. The group =
gives=20
me>>the names of the item(s) they intend to ship. I then =
research how to ship>>these items only. They are trained on =
that=20
subject only. The synopsis>>includes packaging and all=20
information from the CFR49 172.101 table. We>>cover =
shipping=20
papers, shipping containers, labels, markings and =
inner>>containement=20
for that item only. I also include research if the item=20
is>>being exported and the material is TSCA regulated. =
Haven't=20
had one of>>those yet.>>>>Specific =
material=20
training can take as little as 30 minutes. Any time =
a>>different=20
material is to be shipped, we must rain on the new=20
material.>>>>Now, I have two questions that I am =
researching=20
at this time.>>>>I have labs who are shipping human =
blood=20
and blood products. These are not>>diagnostic =
speciments. They=20
are research specimens. Diagnostic specimens>>is a =
Proper=20
Shipping Name(PSN) that is exempt from several=20
regulations.>>>>We do not test for infectious=20
substances. Since we are handling blood,>>Universal =
Precautions=20
and OSHA say to consider the material infectious.>>DOT says =
properly=20
identify the hazard. DO NOT over =
classify.>>>>So=20
should I ship human blood as "Infectious Substance, 6.2" =
or is=20
it>>unregulated?>>>>Shipping=20
containers:>>I am encountering several domestic shipping =
containers=20
that do not have the>>proper markings to indicate that they =
are OK for=20
hazardous materials.>>Instead they are being =
"Certified" for=20
freight shipment with the>>specifications being listed. =
These=20
containers are being provided to us>>from other labs, some =
very=20
prestigeous, and we are being asked to ship our>>specimens in =
these=20
containers. Are these containers=20
acceptable?>>>>Bob>>>>>>=
;>At=20
04:19 PM 4/30/99 -0400, you wrote:>>>>I just received a =
call=20
from a researcher who wants to ship>>>>human blood and =
urine=20
samples to another facility. She>>>>knows the =
proper=20
procedures for packaging and labeling the>>>>specimens. =
However,=20
she is very concerned because she has>>>>not attended an =
IATA=20
approved course on Dangerous=20
Goods>>>>Transportation.>>>>>>T=
he DOT=20
regs state that everyone who offers for shipment dangerous=20
goods>>>must be trained. The type of training is not=20
specified. In the short term>>>she can probably work =
with the=20
shipper to ensure that the material is>>>packaged correctly =
and the=20
paper work is done correctly but she will need>>>to be=20
trained.>>>>>>Richie=20
Fink>>>M.I.T.>>>rfink@mit.edu>>>>>> &nb=
sp; =20
________________________________________________>>__ =
; =20
/ _______________________________________________>>_ =20
\ / /Robert N.=20
Latsch USSF State Referee 6=20
Environmental>>Safety>> \ =
\ /=20
/ 27610 Tremiane Dr. USSF Assessor=20
7 CWRU>> =
\ \/=20
/ Euclid, Ohio, 44132 High School, Indoor =20
Occupational &>> \=20
/ =20
U.S.A. RA=20
Member =
Environmental>>Safety>>> =
&=
nbsp;=20
________________________________________________>__ &nb=
sp; =20
/ _______________________________________________>_ =20
\ / /Robert N.=20
Latsch USSF State Referee 6 Environmental=20
Safety> \ \ / / 27610 =
Tremiane=20
Dr. USSF Assessor 7 =20
CWRU> \ \/ / Euclid, =
Ohio,=20
44132 High School, Indoor Occupational=20
&> \=20
/ =20
U.S.A. RA=20
Member =
Environmental Safety
------=_NextPart_000_0019_01BE9614.4831FB20--
=========================================================================
Date: Tue, 4 May 1999 12:12:53 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "P. Moravek"
Subject: U.S. Course for shipping specimens?
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Can anyone recommend a short (1 day or less) course in the New England, or
Northeast U.S. on shipping of biological & biohazardous specimens?
I'm looking for one that specifically covers all the issues this group has been
going over in the past few weeks, especially U.S. laws.
Thanks.
---P. Moravek, Biological Safety Officer
Worcester Polytechnic Institute
Worcester, MA
=========================================================================
Date: Tue, 4 May 1999 16:21:23 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ninni Jacob
Subject: Autoclaving Infectious Waste
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Dear group:
At our institution, the policy ahs ben that biohazard waste containing
infectious material from the BL3 lab , such as HIV, HBV and MTB are
autoclaved before disposing of them as red bag waste.
All other infectious waste and blood products are redbagged and shipped out.
One of the questions that came up was:
1. If they are being autoclaved, are they rendered non-infectious, (or is
it just an added precaution).
If they are rendered non-infectious, why do they have to be shipped out as
infectious waste?
2. Can all the other waste be autoclaved and rendered non-infectious?
Any comments?
Thanks for your input.
Ninni Jacob, CHP
Radiation and Biological Safety Officer
Office of Risk Management
Brown University - Box 1914
164 Angell Street
Providence, RI 02912
Tel:401 863 1738 (direct)
401 863 3353
Fax:401 863 7676
email: Ninni_Jacob@brown.edu
=========================================================================
Date: Tue, 4 May 1999 16:55:28 -0400
Reply-To: hontzb3@SERVAL.UOFS.EDU
Sender: A Biosafety Discussion List
From: Barbara Hontz
Organization: University of Scranton
Subject: Autoclaving infectious waste
MIME-version: 1.0
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Hi!, My name is Barb Hontz, and I am the lab supervisor for the
University of Scranton in Scranton, PA
Currently, any infectiuos waste that is autoclaved is considered
non-infectious (microbiology lab, Virology, and genetic engineering with
plants) and disposed of in the garbage. We DO NOT allow infectious waste
to be autoclaved in red bags, we put them in clear autoclave bags and
once autoclaved, they go in the garbage.
Other waste goes in a biohazard freezer and is picked up routinely and
incinerated.
This waste usually includes dissected animals as well as any animals
from our animal facility.
Hope this helps!
Barb
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=========================================================================
Date: Tue, 4 May 1999 16:52:44 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ninni Jacob
Subject: CDC Laboratory Registration and Select Agent Transfer
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Dear group:
Our facility is registered with the CDC under 49CFR 72.6. The registration
period is three years.
At the time we applied, we had included only one toxin.
Now researchers are planning to use other toxins.
My question is: do we have to submit the whole application form again for
each toxin? Or do we submit the Select Agent Transfer Form (EA-101), and
wait for CDC to ask us more questions, because the license says: "If you
add select agents... we may request additional information".
Also, do we have to submit the transfer form to CDC each time the PI orders
the same toxin, or do we just keep the forms on file.
If any of you have done this, please email me.
Thanks a lot.
Ninni Jacob, CHP
Radiation and Biological Safety Officer
Office of Risk Management
Brown University - Box 1914
164 Angell Street
Providence, RI 02912
Tel:401 863 1738 (direct)
401 863 3353
Fax:401 863 7676
email: Ninni_Jacob@brown.edu
=========================================================================
Date: Tue, 4 May 1999 16:52:59 +0500
Reply-To: speaker@ehs.psu.edu
Sender: A Biosafety Discussion List
From: Curt Speaker
Organization: UNIVERSITY SAFETY
Subject: Re: Autoclaving Infectious Waste
In-Reply-To:
Ninni:
It probably has a lot to do with what the waste "looks like" versus
it's true infectious potential. Autoclaving kills harmful organisms
but doesn't do a lot for the appearence of the waste: a syringe still
looks like a syringe, a petri dish looks like, well, a shrivelled up
petri dish, tissue culture flasks look like small blobs of plastic, but
the material stills pretty much looks like it did when it went in. Add
to that the fact that it is still inside a red bag labelled with a
biohazard symbol, and you have the potential for problems if you
sent it to the landfill.
Some folks are now going to a hammermill-type shredder to render
their autoclaved waste unrecognizable --- it can then be sent to the
landfill with few problems.
Just my $0.02...
Curt
Curt Speaker
Biosafety Officer
Penn State University
Environmental Health and Safety
speaker@ehs.psu.edu
^...^
(O_O)
=(Y)=
"""
=========================================================================
Date: Tue, 4 May 1999 17:07:51 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Autoclaving Infectious Waste
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
I cannot answer that question for you directly. I can only tell you what
is done in Ohio.
Medical Waste can be treated and rendered non-infectious under state law by
autoclaving. Once this is done the waste is considered regular trash. The
catch is that the stae has a set of regulations about autoclave use that we
must comply wirh. We consider the regulations to be to burdensome.
We autoclave infectious waste to make it easier to handle. Since we do not
comply with teh state autoclave regulations, it is still considered to be
infectious.
BBP are easier. Sterilization renders the material non-infectious and is
therefore not regulated.
Bob
>Dear group:
>
>At our institution, the policy ahs ben that biohazard waste containing
>infectious material from the BL3 lab , such as HIV, HBV and MTB are
>autoclaved before disposing of them as red bag waste.
>All other infectious waste and blood products are redbagged and shipped out.
>
>One of the questions that came up was:
>1. If they are being autoclaved, are they rendered non-infectious, (or is
>it just an added precaution).
>If they are rendered non-infectious, why do they have to be shipped out as
>infectious waste?
>
>2. Can all the other waste be autoclaved and rendered non-infectious?
>
>Any comments?
>
>Thanks for your input.
>
>
>Ninni Jacob, CHP
>Radiation and Biological Safety Officer
>Office of Risk Management
>Brown University - Box 1914
>164 Angell Street
>Providence, RI 02912
>
>Tel:401 863 1738 (direct)
> 401 863 3353
>Fax:401 863 7676
>
>email: Ninni_Jacob@brown.edu
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Tue, 4 May 1999 14:12:45 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Terri Hellman
Subject: Re: CDC Laboratory Registration and Select Agent Transfer
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
I believe you need to submit an amendment. Call Nasr Gergis at CDC Select
Agent Registration. He is very helpful. 404/639-4420
Ninni Jacob wrote:
> Dear group:
>
> Our facility is registered with the CDC under 49CFR 72.6. The registration
> period is three years.
>
> At the time we applied, we had included only one toxin.
>
> Now researchers are planning to use other toxins.
>
> My question is: do we have to submit the whole application form again for
> each toxin? Or do we submit the Select Agent Transfer Form (EA-101), and
> wait for CDC to ask us more questions, because the license says: "If you
> add select agents... we may request additional information".
>
> Also, do we have to submit the transfer form to CDC each time the PI orders
> the same toxin, or do we just keep the forms on file.
>
> If any of you have done this, please email me.
>
> Thanks a lot.
>
> Ninni Jacob, CHP
> Radiation and Biological Safety Officer
> Office of Risk Management
> Brown University - Box 1914
> 164 Angell Street
> Providence, RI 02912
>
> Tel:401 863 1738 (direct)
> 401 863 3353
> Fax:401 863 7676
>
> email: Ninni_Jacob@brown.edu
=========================================================================
Date: Tue, 4 May 1999 17:16:36 EDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Re: Autoclaving Infectious Waste
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Ninni;
Rule #1 regarding medical waste regulations
Some of the rules are based upon perception rather than science. Perception
has become reality in various parts of the country (but it is becomming
better!!).
Rule #2 regarding medical waste regulations
Check with your state DEM regarding RI regs. What counts first is what your
state allows (and sometime what your local government allows - i.e., All
clinical micro labs in NYC must autoclave their waste)
Contact Bob Schmidt of the RIDEM @ 401-222-2797 x7260
Rule #3 regarding medical waste regulations
If your "treated waste" leaves RI for disposal elsewhere, it may be subject
to different requirements such as in Connecticut, medical waste must be
treated and destroyed.
Virginia has some of the most restrictive regulations. Even treated waste
must be placed in an opaque bag. The governor has entered the trash wars!!
Regards,
Ed Krisiunas, MT(ASCP), CIC, MPH
Director
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
In a message dated 5/4/99 4:20:56 PM, Ninni_Jacob@BROWN.EDU writes:
=========================================================================
Date: Wed, 5 May 1999 13:32:09 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Cohen, Barry"
Subject: Job Opportunity
PLEASE REPLY TO THE CONTACT LISTED BELOW. DO NOT REPLY TO THE LIST
======================================================================
Please be advised that this position is heavy on environmental
qualifications (EPA, Mass DEP). Health and Safety (OSHA, etc.) is not
included in the scope of the position.
Employment Opportunity
Environmental Engineer
Genzyme Corporation
76 New York Avenue
Framingham, MA 01710
Contact:
Joan Boegel
Manager, Environmental Engineering
(508) 270-2176
jboegel@
Corporation Overview
Founded in 1981, Genzyme Corporation is a healthcare products company that
focuses on developing and providing innovative solutions to major unresolved
medical needs. The Corporation is organized into four primary business
areas-- biotherapeutics, surgical products, diagnostic products & services,
and pharmaceuticals and fine chemicals. The corporation's international
headquarters are located in Cambridge, Massachusetts, with production and
research facilities in Boston, Cambridge and Framingham, Massachusetts.
Internationally, the corporation is located in Maidstone and Haverhill,
England; Russelsheim Germany, Italy, The Netherlands, Belgium, Switzerland
and Japan. The Company currently employs approximately 3500 people
worldwide.
Description:
Ensure compliance with environmental regulations & permits; evaluate
environmental permitting requirements for Genzyme facilities, equipment &
processes at all locations; conduct environmental audits & recommend
corrective actions; provide technical assistance regarding environmental
issues to operations managers & project engineers.
Experience/Skills Required:
5-10 years experience in environmental compliance for manufacturing and/or
lab operations; familiarity with Clean Air Act & air emissions permitting,
wastewater regulations, hazardous waste mgmt., spill response, pollution
prevention & environmental auditing is required; excellent problem solving &
communication skills essential.
Desired Education:
BS degree in Environmental Engineering or related engineering discipline.
=========================================================================
Date: Thu, 6 May 1999 09:01:14 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Lauer
Subject: Re: Autoclaving Infectious Waste
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
At 4:21 PM -0400 5/4/99, Ninni Jacob wrote:
>Dear group:
>
>At our institution, the policy ahs ben that biohazard waste containing
>infectious material from the BL3 lab , such as HIV, HBV and MTB are
>autoclaved before disposing of them as red bag waste.
>All other infectious waste and blood products are redbagged and shipped out.
>
>One of the questions that came up was:
>1. If they are being autoclaved, are they rendered non-infectious, (or is
>it just an added precaution).
>If they are rendered non-infectious, why do they have to be shipped out as
>infectious waste?
>
>2. Can all the other waste be autoclaved and rendered non-infectious?
>
>Any comments?
>
>Thanks for your input.
>
>
>Ninni Jacob, CHP
>Radiation and Biological Safety Officer
>Office of Risk Management
>Brown University - Box 1914
>164 Angell Street
>Providence, RI 02912
>
>Tel:401 863 1738 (direct)
> 401 863 3353
>Fax:401 863 7676
>
>email: Ninni_Jacob@brown.edu
We (U of MN) have a policy that if you autoclave infectious waste, it can
then be disposed of as non-infectious waste ( regular waste), except for
Sharps. However, note that we require 55 to 60 minutes at 121 degrees C.
Jim Lauer
University of Minnesota
Environmental Health & Safety
W-158
626-5621
lauer001@maroon.tc.umn.edu
=========================================================================
Date: Thu, 6 May 1999 13:35:13 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Francis Churchill
Subject: Re: Autoclaving Infectious Waste
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Here at the University of Vermont, laboratorians autoclave most of the
infectious waste, also tissue culture waste and other material that "looks"
biohazardous. That material can be red bagged or not. After autoclaving,
it is placed in a separate locked dumpster for "DISINFECTED BIOLOGICAL
MATERIAL ONLY." We have 5 of these dumpsters on campus and they are
emptied every other week. They are not commingled with other trash, but
are taken to a landfill where they are immediately buried. This is our
agreement with the landfill operators, the State doesn't see this material
has hazardous waste.
A small percentage of our biowaste is sent for incineration at a medical
waste incinerator (BFI). This happens when a lab doesn't have access to an
autoclave, when the material is particularly nasty (encephalitis infected
brain samples), or looks particularly gross (joints and such - though we
haven't seen this for several years now).
We have an on campus crematorium for animals carcasses. Human remains are
cremated off campus (often from whence they came).
I think that's about it,
Francis
=========================================================================
Date: Fri, 7 May 1999 17:45:47 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Andrew Braun
Subject: Call for ABSA Papers
MIME-Version: 1.0
Content-Type: text/plain; charset=ISO-8859-1
Content-Transfer-Encoding: 7bit
Dear Biosaftyers,
This is a reminder that the American Biological Safety
Association (ABSA) will be meeting October 17-20, 1999 at the Regal
Riverfront Hotel in St. Louis, MO. The program is shaping up well but we
would like to have as many presentations as possible. If you have something
interesting to tell your colleagues at the meeting please send in an
abstract. It will be greatly appreciated The more
presentations, the more interesting and valuable the meeting. Try to
have your abstract in by Memorial Day so it can go in the program book.
So send an abstract for your talk, by e-mail, to Andy Braun at
abraun@. (Don't send it to Biosafety, you'll give it away
and no one will come to St. Louis!). You can also get in touch with me
at 617-726-5680, FAX 617-726-5705.
Please make sure you put in the following:
a) the title,
b) the authors' names (indicating who will give the
presentation),
c) the authors' addresses,
d) a one paragraph abstract covering why, what and conclusions,
e) whether you want a platform or a poster presentation (or
either),
f) whether you are interested in trying an electronic
presentation (computer video) and
g) whether you have some financial connection with any product
or services discussed in the presentation (if you do this doesn't
disqualify you - it's just for information purposes).
Andy Braun
ABSA Scientific Program Chair
=========================================================================
Date: Mon, 10 May 1999 11:36:06 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Melinda Young
Organization: Primate Center U of W
Subject: NIH guidelines and oncogenes
MIME-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
Good morning:
The Chair of our IBC has asked me to find out if other IBC's
have written policy on the use of oncogenes in recombinant vectors. The
guidelines say that in these cases consideration should be given to
increasing containment. Our first reaction would be that if the vector is
a BL-2 agent and the insert contains a known oncogene that this means BL-2
facilities with BL-3 practices.
Do you define the type of BL-2 facility? Do you have design
standards/facility standards for labs that are more than BL-2(BL-2+ or
BL-2.5)?
We have multi-user BL-2 labs and it seems to me that you can't be
following BL-2 and BL-3 practices in the same space.
Thank you.
Melinda Young
______________________________________________________________________________
Melinda A. Young, CBSP University of Washington
Health and Safety Coordinator Box 357330
Regional Primate Research Center Seatttle, WA 98195-7330
Phone: (206) 543-8686
melinday@bart.rprc.washington.edu
biosafe@u.washington.edu
Melinda Young Phone(206)543-8686
Health and Safety Coordinator
Regional Primate Research Center
University of Washington
Box 357330
Seattle, WA 98195-7330
Melinda Young Phone(206)543-8686
Health and Safety Coordinator
Regional Primate Research Center
University of Washington
Box 357330
Seattle, WA 98195-7330
=========================================================================
Date: Mon, 10 May 1999 16:44:09 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Melinda Young
Organization: Primate Center U of W
Subject: Special care.
MIME-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
Greetings:
The Chair of our IBC has asked me to find out if other IBC's
have written policy on the following section of the NIH Guidelines.
Section III-D-3. Experiments Involving the Use of Infectious DNA or
RNA Viruses or Defective DNA or RNA Viruses in the Presence of Helper
Virus in Tissue Culture Systems
Caution: Special care should be used in the evaluation of containment
levels for experiments which are likely to either enhance the
pathogenicity (e.g., insertion of a host oncogene) or to extend the
host range(e.g., introduction of novel control elements) of viral
vectors under conditions that permit a productive infection. In such
cases, serious consideration should be given to increasing physical
containment by at least one level.
Our first reaction would be that if the vector is a BL-2 agent and
the insert contains a known oncogene that this means BL-2 facilities
with BL-3 practices.(Or BL-3 practices and facilities?)
Do you define the type of BL-2 facility? Do you have design
standards/facility standards for labs that are more than BL-2(BL-2+ or
BL-2.5)?
We have multi-user BL-2 labs and it seems to me that you can't be
following BL-2 and BL-3 practices in the same space.
Thank you.
Melinda Young
______________________________________________________________________________
Melinda A. Young, CBSP University of Washington
Health and Safety Coordinator Box 357330
Regional Primate Research Center Seatttle, WA 98195-7330
Phone: (206) 543-8686
melinday@bart.rprc.washington.edu
biosafe@u.washington.edu
Melinda Young Phone(206)543-8686
Health and Safety Coordinator
Regional Primate Research Center
University of Washington
Box 357330
Seattle, WA 98195-7330
=========================================================================
Date: Mon, 10 May 1999 16:28:53 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Robin Mecklem
Subject: Biowaste Management Survey
Mime-Version: 1.0
Content-Type: text/enriched; charset="us-ascii"
Biosafety Discussion List Members:
I am writing to request your assistance. In addition to being a fellow
biosafety professional, I am in the process of finishing my Master's
degree in Environmental Health Management. To meet thesis requirements,
I plan to conduct a survey of university environmental health and safety
professionals to determine current methods and policies for biowaste
management in this unique environment. There are 2 questions that I am
hoping to answer by posting this message, and these are listed below. I
will GREATLY appreciate any feedback that anyone is willing to provide!
***** Please respond directly to me by email at:
mecklem@pilot.msu.edu *****
1. Are you aware of any previously conducted surveys that are related to
biohazardous waste practices in university environments?
2. If I conduct such a survey, what information would you be most
interested in seeing in a final report based on this survey?
Thank you all in advance for your assistance.
Robin Mecklem
Assistant Biological Safety Officer
The Office of Radiation, Chemical and Biological Safety
Michigan State University
C-124 Research Complex-Engineering
East Lansing, MI 48824
(517) 355-1283
mecklem@pilot.msu.edu
=========================================================================
Date: Mon, 10 May 1999 14:04:06 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Karen Ruhl
Subject: High Titer HIV
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Dear All:
At what concentration of HIV would one start using BSL3?, In other words
what is considered "high" titer and what is "normal"? If the volume is
low, 100 ml, but the titer high, would it be acceptable to use BSL3
practices in a BSL2 room?
Please reply directly to me at karenr@chugai.
Thanks.
Karen Ruhl
Manager, EH&S
Chugai Biopharmaceuticals, Inc.
San Diego, CA
=========================================================================
Date: Mon, 10 May 1999 21:00:57 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Fran Martin
Subject: Most Current U.S. CFRs on CD-ROM (incl. Titles 21,40,42)
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
FYI for those who require instant access to U.S. regulations,
The U.S. Code of Federal Regulations (all 50 Titles) updated to the latest
1998 revision levels are now available on CD-ROM.
Features include no "time locks," 5,000+ in-line graphics, Adobe(R)
Acrobat(R) PDF format, and instant search/retrieval.
See for details.
Fran Martin
FM Research & Consulting
=========================================================================
Date: Tue, 11 May 1999 08:10:09 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Erik Talley
Subject: Re: Most Current U.S. CFRs on CD-ROM (incl. Titles 21,40,42)
MIME-Version: 1.0
Content-Type: text/plain
And for those of you who require the most up to date instant access to
CFR's absolutely free, you can find them at the National Archives and
Records Administration (part of Government Printing Office). Their
website is:
If you haven't been to the site recently, you should check it out.
They've changed the format significantly. Now you can get both PDF and
TXT files of the CFR's, they are set up in Table of Contents or Text
Search and you can get the current version and up to 4 past versions
on-line.
Erik
___________________________________
Erik A. Talley, Assistant Director
Center for Environmental Health and Safety
Southern Illinois University Carbondale
erik@cehs.siu.edu
-----Original Message-----
From: Fran Martin [mailto:fmartin@]
Sent: Monday, May 10, 1999 8:01 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Most Current U.S. CFRs on CD-ROM (incl. Titles 21,40,42)
FYI for those who require instant access to U.S. regulations,
The U.S. Code of Federal Regulations (all 50 Titles) updated to the
latest
1998 revision levels are now available on CD-ROM.
Features include no "time locks," 5,000+ in-line graphics, Adobe(R)
Acrobat(R) PDF format, and instant search/retrieval.
See for details.
Fran Martin
FM Research & Consulting
=========================================================================
Date: Tue, 11 May 1999 13:45:38 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Cagle, Donald W"
Subject: Training Materials
Is anyone aware of a good video that discusses/reviews the proper use and
care of class II biosafety cabinets? I have used "Safe Use of Biological
Safety Cabinets - or The Case of the Contaminated Cultures" from the
Eagleson Institute for previous training sessions. It is soon time to
conduct refresher training and I hate to re-use the same video(s) over and
over. Thanks in advance.
=========================================================================
Date: Tue, 11 May 1999 11:13:41 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Nick Rinaldi
Subject: Job Announcement - San Diego, CA
Mime-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
TWO ENTRY-LEVEL INDUSTRIAL HYGIENE POSITIONS OPEN
Occupational Services, Inc. is actively seeking to fill two (2) entry-lev=
el
positions which require 1-2 years experience and a B.S. or M.S. in Indust=
rial
Hygiene, Health Physics or Environmental & Occupational Health. Strong
writing,
communication, and training skills are required. Furthermore, the applica=
nts
must be able to work independently in a productive small business environ=
ment.
We want individuals with an entrepreneurial spirit. Applicants must also =
be
proficient using Microsoft Word, Excel, PowerPoint, and Access.=A0=20
Most importantly, we are looking for individuals with a strong desire to
advance their careers in Health and Safety. The successful candidates for=
this
position will gain valuable experience and skills under the supervision o=
f
senior staff members.
Occupational Services, Inc. (OSI) is a growing health and safety consulti=
ng
company located in San Diego.=A0 Established in 1988, OSI provides indust=
rial
hygiene, safety, and environmental compliance services to research
laboratories, hospitals, and manufacturing facilities throughout Southern
California.=A0 The firm is staffed primarily with trained and degreed ind=
ustrial
hygienists and health physicists.
The successful candidates will provide services in the areas of laborator=
y
safety, biological safety, chemical safety, and radiation safety.=A0 More
specifically, the candidates will:
- Provide safety training courses
- Complete industrial hygiene and ventilation surveys
- Act as a liaison during regulatory inspections
- Arrange the proper disposal of hazardous, radioactive, and biohazardous
waste
- Conduct monthly radiation contamination surveys
- Review radiation safety programs and amend radioactive materials licens=
es
- Prepare written safety plans
- Inspect facilities for safety compliance
- Write detailed inspection reports
- Obtain and maintain environmental permits
Interested candidates should fax or email their resume and their salary
requirements to:
Occupational Services, Inc.,=20
Fax: (619) 558-6756
Email: admin@
11230 Sorrento Valley Road, Suite 160
San Diego, CA=A0 92121=20
Nick Rinaldi
voice: (619) 252-2211
fax: (619) 558-6756
Occupational Services, Inc.
11230 Sorrento Valley Rd. Suite 160
San Diego, CA 92121
=========================================================================
Date: Wed, 12 May 1999 09:22:39 +0200
Reply-To: peter.mani@ivi.admin.ch
Sender: A Biosafety Discussion List
From: Peter Mani
Subject: decon of full body suits
Mime-Version: 1.0 (NeXT Mail 4.2mach v148)
Content-Type: multipart/alternative; boundary=NeXT-Mail-1207319190-2
Content-Transfer-Encoding: 7bit
--NeXT-Mail-1207319190-2
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Which chemicals are usually used to decon full body suits in chemical showers?
How are the chemicals neutralized?
Thanks for every information.
------------------ ------------------------ --
Dr. Peter Mani
Head Biosafety
Institute of Virology and Immunoprophylaxis
P.O. Box
CH-3147 Mittelhaeusern
SWITZERLAND
Phone: +41-31-8489 234
Fax: +41-31-8489 222 or
Natel: 079 - 675 0581 (not at IVI)
E-mail: peter.mani@ivi.admin.ch
_______________________________________________
--NeXT-Mail-1207319190-2
Content-type: text/enriched; charset="us-ascii"
Content-transfer-encoding: 7bit
Content-Disposition: inline
Which chemicals are usually used to decon full body suits in chemical
showers?
How are the chemicals neutralized?
Thanks for every information.
------------------ ------------------------ --
Dr. Peter Mani
Head Biosafety
Institute of Virology and Immunoprophylaxis
P.O. Box
CH-3147 Mittelhaeusern
SWITZERLAND
Phone: +41-31-8489 234
Fax: +41-31-8489 222 or
Natel: 079 - 675 0581 (not at IVI)
E-mail: peter.mani@ivi.admin.ch
_______________________________________________
--NeXT-Mail-1207319190-2--
=========================================================================
Date: Wed, 12 May 1999 14:15:13 +0200
Reply-To: peter.mani@ivi.admin.ch
Sender: A Biosafety Discussion List
From: Peter Mani
Subject: decon of full body suits
Content-Type: text/plain
Mime-Version: 1.0 (NeXT Mail 4.2mach v148)
Which chemicals are usually used to decon full body suits in chemical showers?
How are the chemicals neutralized?
Thanks for every information.
------------------ ------------------------ --
Dr. Peter Mani
Head Biosafety
Institute of Virology and Immunoprophylaxis
P.O. Box
CH-3147 Mittelhaeusern
SWITZERLAND
Phone: +41-31-8489 234
Fax: +41-31-8489 222 or
Natel: 079 - 675 0581 (not at IVI)
E-mail: peter.mani@ivi.admin.ch
_______________________________________________
=========================================================================
Date: Wed, 12 May 1999 08:40:33 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: decon of full body suits
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
I have never had to do this. From my training with chemical spills,
decontaimination is normaly done with soap. If I were working a biological
spill I would probably use a bleach solution.
Bob
>Which chemicals are usually used to decon full body suits in chemical showers?
>How are the chemicals neutralized?
>
>Thanks for every information.
>
>------------------ ------------------------ --
>Dr. Peter Mani
>Head Biosafety
>Institute of Virology and Immunoprophylaxis
>P.O. Box
>CH-3147 Mittelhaeusern
>SWITZERLAND
>
>Phone: +41-31-8489 234
>Fax: +41-31-8489 222 or
>Natel: 079 - 675 0581 (not at IVI)
>E-mail: peter.mani@ivi.admin.ch
>_______________________________________________
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Wed, 12 May 1999 14:57:22 +0200
Reply-To: peter.mani@ivi.admin.ch
Sender: A Biosafety Discussion List
From: Peter Mani
Subject: decon of full body suits
Mime-Version: 1.0 (NeXT Mail 4.2mach v148)
Content-Type: multipart/alternative; boundary=NeXT-Mail-1408889577-3
Content-Transfer-Encoding: 7bit
--NeXT-Mail-1408889577-3
Content-type: text/plain; charset="us-ascii"
Content-transfer-encoding: 7bit
Content-Disposition: inline
Sorry, I was probably a bit to short in formulating my question: I am not
talking about chemical spills, I am talking about a level 4 lab (BL-4) where
personel is working in full body suits. These suits have to be decontaminated
in a chemical shower before opening the suit and taking a conventionel
shower (manily for hygenic reasons). I am currently involved in the planing
of such a lab in Switzerland and was wondering which chemicals are normally
being used in the chemivcal shower and how do I have to treat that chemicals
before leding it to the public sewage.
------------------ ------------------------ --
Dr. Peter Mani
Head Biosafety
Institute of Virology and Immunoprophylaxis
P.O. Box
CH-3147 Mittelhaeusern
SWITZERLAND
Phone: +41-31-8489 234
Fax: +41-31-8489 222 or
Natel: 079 - 675 0581 (not at IVI)
E-mail: peter.mani@ivi.admin.ch
_______________________________________________
>you wrote
I have never had to do this. From my training with chemical spills,
decontaimination is normaly done with soap. If I were working a biological
spill I would probably use a bleach solution.
Bob
>Which chemicals are usually used to decon full body suits in chemical showers?
>How are the chemicals neutralized?
>
>Thanks for every information.
>
--NeXT-Mail-1408889577-3
Content-type: text/enriched; charset="us-ascii"
Content-transfer-encoding: 7bit
Content-Disposition: inline
Sorry, I was probably a bit to short in formulating my question: I am not
talking about chemical spills, I am talking about a level 4 lab (BL-4)
where personel is working in full body suits. These suits have to be
decontaminated in a chemical shower before opening the suit and taking a
conventionel shower (manily for hygenic reasons). I am currently involved
in the planing of such a lab in Switzerland and was wondering which
chemicals are normally being used in the chemivcal shower and how do I
have to treat that chemicals before leding it to the public sewage.
------------------ ------------------------ --
Dr. Peter Mani
Head Biosafety
Institute of Virology and Immunoprophylaxis
P.O. Box
CH-3147 Mittelhaeusern
SWITZERLAND
Phone: +41-31-8489 234
Fax: +41-31-8489 222 or
Natel: 079 - 675 0581 (not at IVI)
E-mail: peter.mani@ivi.admin.ch
_______________________________________________
>you wrote
I have never had to do this. From my training with chemical spills,
decontaimination is normaly done with soap. If I were working a biological
spill I would probably use a bleach solution.
Bob
>Which chemicals are usually used to decon full body suits in chemical showers?
>How are the chemicals neutralized?
>
>Thanks for every information.
>
--NeXT-Mail-1408889577-3--
=========================================================================
Date: Wed, 12 May 1999 11:30:32 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Madeline J. Dalrymple"
Subject: Human Research Shut Down at Duke University
MIME-version: 1.0
Content-type: text/plain; charset="iso-8859-1"
The U.S. government has temporarily shut down federally funded research on
humans at Duke University Medical Center, one of the nation's largest and
most prestigious medical research facilities, after federal investigators
determined that the university could not ensure the safety of participants.
For more info go to:
Madeline Dalrymple
Biological Safety Officer
University of Wyoming Environmental Health and Safety Office
Laramie, Wyoming, USA
307-766-2723, fax 307-766-5678
dalrympl@uwyo.edu
=========================================================================
Date: Thu, 13 May 1999 08:53:13 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Meechan, Paul J."
Subject: MABSA Biosafety Symposium
MIME-version: 1.0
Content-type: text/plain
Content-transfer-encoding: 7BIT
On Wednesday, June 2nd MABSA will be holding their 12th annual Biosafety
Symposium at the University of Delaware Newark, DE campus. Below is the
tentative agenda.
Topic Speaker
Herpes B Taskforce Discussion Dr. Jonathan Richmond, CDC
Avian (hong Kong) flu Dr. Jack Rosenberger, Univ. Del.
Allen Biotechnology Lab tours and Presentations
Brucellosis/TB in Large Animals and Transmission Scott Rusk, USDA
to Humans
Antimicrobial Resistance Don Lehman, Univ. Delaware
Large Animal Containment and Medical Surveillance Scott Rusk, USDA
All members of the biosafety community are invited to attend and network
with their colleagues in the region. For registration details, please
contact one of the following MABSA Council members:
Leslie Delpin (Treasurer) 860-486-3613
lmdelpin@safety.ehs.uconn.edu
Paul Meechan (Secretary) 215-652-0744 meechan@
Harriet Izenberg (President) 215-898-4453 harriet@ehrs.upenn.edu
Paul J. Meechan, Ph.D.
Biosafety Manager, WP
Merck & Co., Inc.
215-652-0744
meechan@
=========================================================================
Date: Thu, 13 May 1999 14:14:42 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Gill Norton
Organization: University of Western Ontario
Subject: decontamination of an animal room
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
I am looking for some advise on how to decontaminate animal housing
rooms that have viral contamination from MPV and MHV. We want to decon
the room and put clean animals into it. Formaldehyde gas or Clidox
fogging has been suggested. The contaminated animal room are part of a
working animal housing facility so the contaminated rooms would have to
be decontaminated while the adjacent rooms are in use.
Anyone with experience doing this, please contact me at my e mail adress
gmnorton@julian.uwo.ca or to jcator@julian.uwo.ca
Thanks.
--
------------------------------------------------------------------
Gillian Norton
Biosafety Officer
The University of Western Ontario
Occupational Health and Safety
Somerville House, Rm 2345
Phone: (519)661-2036
FAX: (519)661-3420
-------------------------------------------------------------------
=========================================================================
Date: Thu, 13 May 1999 13:47:32 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Kyle Boyett
Subject: Re: decontamination of an animal room
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Gillian, You mention that the rooms are apparently connected. If this is the
case how? Are they connected by common ventilation or are they connected by
a lack of walls or partitions? If the adjacent rooms are all under negative
pressure in addition to this room and this room can be effectively isolated
from the other rooms, I see no reason why this room cannot be "gassed" with
paraformaldehyde. Evacuation (of the gas) procedures should be taken into
account for this process. You may want to entertain the idea of using B.
subtilis or B. stearothermophilus spore strips as a test to make sure the
facility has been decontaminated properly. Personally I prefer using
subtilis since this is basically a modified procedure used for biosafety
cabinet decon. Also keep in mind if you decide to use the paraformaldehyde
gas that there tends to be a residue after the procedure that could
potentially pose a problem if not cleaned. Hope this helps.
Kyle Boyett
Asst. Director of Biosafety
Occupational Health and Safety
University of Alabama at Birmingham
e-mail- kboyett@healthsafe.uab.edu
Phone- 205-934-2487
** Asking me to overlook a safety violation is like asking me to reduce the
value I place on YOUR life**
-----Original Message-----
From: Gill Norton [mailto:gmnorton@JULIAN.UWO.CA]
Sent: Thursday, May 13, 1999 1:15 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: decontamination of an animal room
I am looking for some advise on how to decontaminate animal housing
rooms that have viral contamination from MPV and MHV. We want to decon
the room and put clean animals into it. Formaldehyde gas or Clidox
fogging has been suggested. The contaminated animal room are part of a
working animal housing facility so the contaminated rooms would have to
be decontaminated while the adjacent rooms are in use.
Anyone with experience doing this, please contact me at my e mail adress
gmnorton@julian.uwo.ca or to jcator@julian.uwo.ca
Thanks.
--
------------------------------------------------------------------
Gillian Norton
Biosafety Officer
The University of Western Ontario
Occupational Health and Safety
Somerville House, Rm 2345
Phone: (519)661-2036
FAX: (519)661-3420
-------------------------------------------------------------------
=========================================================================
Date: Fri, 14 May 1999 14:34:00 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Mann
Subject: House hold Ammonia
Does anyone have any information on the usefulness or not or household
ammonia for killing the mouse louse and the mouse pinworm?
Thanks
Richard Mann, DVM
VMO
VA Northport, NY
516 261 4400 x 2878
mann.richard@northport.
=========================================================================
Date: Fri, 14 May 1999 16:08:22 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: decontamination of an animal room
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
The best way I have heard of is a paraformaldehyde candle. You need to
shut off the ventilation, Light the candle(this gives off formaldehyde) let
it flood the room and then turn on the ventilation.
One problem, many older buildings will have general room air exhaust and
chemical fume hood exhausts. Some general room air exhausts do not exhaust
all of the air. Some older buildings will re-circulate room exhaust air to
avoid warming 100% supply air from outside. This was a popular energy
saving feature even in laboratory buildings.
Bob
>I am looking for some advise on how to decontaminate animal housing
>rooms that have viral contamination from MPV and MHV. We want to decon
>the room and put clean animals into it. Formaldehyde gas or Clidox
>fogging has been suggested. The contaminated animal room are part of a
>working animal housing facility so the contaminated rooms would have to
>be decontaminated while the adjacent rooms are in use.
>
>Anyone with experience doing this, please contact me at my e mail adress
>gmnorton@julian.uwo.ca or to jcator@julian.uwo.ca
>Thanks.
>--
>------------------------------------------------------------------
>Gillian Norton
>Biosafety Officer
>The University of Western Ontario
>Occupational Health and Safety
>Somerville House, Rm 2345
>Phone: (519)661-2036
>FAX: (519)661-3420
>-------------------------------------------------------------------
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Fri, 14 May 1999 13:35:22 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Elizabeth Smith
Subject: Re: decontamination of an animal room
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
My experience is based on paraformaldehyde fumigation of medium
sized areas in an isolated building.
Things I would consider for the decon of labs with fumigation:
1. How is the building ventilation constructed? Can you
achieve an adequate seal of the room to be deconned? You don't
want unintentional exposure to the formaldehyde for the animals
next door. I wouldn't rely on pressure differentials to contain
the formaldehdye. You need to be able to physically seal the
room to keep the formaldehyde in and thereby ensure a thorough
exposure to all of the surfaces at a concentration high enough
to kill all of the unwanted organisms.
2. How are you going to verify that the room is adequately
decontaminated? My facility uses B. subtilis v. niger when
doing paraformaldehyde decontamination of our animal room which
has had B. anthracis in it. Put the biological indicator out
during the decon - if it's dead at the end, so is just about
everything else.
3. how fast do you need it decontaminated? When using a
biological indicator, make sure you include the testing time at
the end of the decon when providing a date for alteration to the
use/access.
4. When we use paraformaldehyde to decon a room, we follow the
fumigation with a second fumigation with an ammonium salt
(carbonate or bicarb), to react with the formaldehyde. The
product of the formaldehyde/ammonia is innocuous, water soluble,
and easy to clean up.
5. If you can't adequately seal the room that is being
fumigated to protect the animals next door, can you move the
animals for the duration of the fumigation? Check with your
staff veterinarian (or equivalent responsible party) for the
answer to this.
If you are interested, I can provide you with a copy of the full
written procedure we follow. It is based on some communication
with the CDC in years past. I'm at safety_queen@ or
e_e_smith@.
===
Elizabeth Smith
Environmental, Health & Safety
BioPort Corporation
3500 N. Martin L. King Blvd.
Lansing, MI 48906
517-327-1590
_____________________________________________________________
Do You Yahoo!?
Free instant messaging and more at
=========================================================================
Date: Mon, 17 May 1999 16:14:02 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
Comments: RFC822 error: Incorrect or incomplete address field found and
ignored.
From: Gary Cseko
Subject: IBC bylaws
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: quoted-printable
We are in the process of writing bylaws for our institutional biosafety =
committee and I have a few questions for the group:
If the IBC at your institution is larger than the required 5 members (NIH =
Guidelines), how many members are needed to review and approve a protocol? =
Also, do you require a unanimous vote to approve a protocol or will a =
majority suffice?
Are there any good models for IBC bylaws out there?
If you would prefer responding directly to me, I'd be happy to summarize =
responses and post. Thank you for your input.
Gary C. Cseko, MSPH
SOM Safety Compliance Officer
BMSB Room B77
UNM School of Medicine
Albuquerque, NM 87131-5196
(505) 272-1492
(505) 272-8738 (Fax)
=========================================================================
Date: Tue, 18 May 1999 08:35:20 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: IBC bylaws
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
At 04:14 PM 5/17/99 -0600, you wrote:
>We are in the process of writing bylaws for our institutional biosafety
committee and I have a few questions for the group:
>
Gary C. Cseko, MSPH
>SOM Safety Compliance Officer
>UNM School of Medicine
>Albuquerque, NM 87131-5196
I don't think we have any formal bylaws. Our IBC operates per normal
committee rules - a majority of members are needed for a quorum and a
quorum is needed to conduct business. A majority of the members present
must approve a protocol.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Wed, 19 May 1999 08:25:45 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: TSawicki
Subject: Re: decontamination of an animal room -Reply
Mime-Version: 1.0
Content-Type: text/plain
Please provide a vendor where the formaldehyde candle can be
purchased? Is the product registered with the EPA and your State for
use as a pesticide? Regards-
Thomas Sawicki, Safety Officer
USDA ARS PIADC
=========================================================================
Date: Wed, 19 May 1999 09:36:42 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: customer satisfaction surveys
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I have found one on the UCSF website, but I wondered if there were others
out there who would be willing to share their efforts in this direction.
Reply directly to me please:
therese.stinnett@uchsc.edu
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
=========================================================================
Date: Wed, 19 May 1999 15:55:09 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stefan Wagener
Subject: Biosafety Position at MSU!!!
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Biosafety Position (IH) at Michigan State University
Michigan State University, Office of Radiation, Chemical and Biological Safety
(ORCBS) is seeking an experienced and motivated individual to join its team of
health and safety professionals as an Industrial Hygienist. This highly visible
and responsible position will assist in the development, implementation and
maintenance of a comprehensive biological safety program.
Founded in 1855, MSU is a research-intensive land-grant university where people
matter. The curriculum, which originally concentrated on farm science, now
includes more than 200 programs of undergraduate and graduate studies, all
taught by nearly 4,000 academic staff in 14 degree-granting colleges. MSU, a
Big-Ten University operates on the semester system and has currently more than
40,000 enrolled students.
The successful candidate will assist with the control and minimization of risk
involved in biological agents, assuring that the safety and compliance
requirements are met for use, storage, transport and disposal of biohazardous
materials at Michigan State University.
Duties for which this person will be responsible include, but are not limited
to: development and maintenance of a comprehensive program to control and
eliminate biological hazards; provide site and function specific training;
review research projects and inspects facilities related to research, teaching,
outreach and support operations for compliance with applicable rules,
regulations and guidelines; provides assistance to University units in the
development and implementation of internal biological and related biosafety
programs; serves as an on-call technical advisor to the University for
emergencies and incidents involving biohazardous materials. The position
involves handling biological agents and biohazardous materials including
bloodborne pathogens, wearing a respirator, and working on a VDT 25% of the
time.
Minimum requirements for the qualified candidate are a Bachelor's degree in
Industrial Hygiene/Environmental Health and Safety, or a closely related field;
two years of related and progressively more responsible or expansive experience
in biological safety; demonstrated ability to know and apply safety
regulations; experience in safety requirements for research laboratories;
safety training program development and presentation; computer literacy; or an
equivalent combination of education and experience. Desired qualifications
include: Laboratory research experience and microbiology education. The minimum
salary is $ 32,073/yr.
To apply for this position, call the Michigan State University Employment
Office at (517) 432-1662. Refer to Posting Number P90373. The closing date is
May 25, 1999.
Michigan State University is an Affirmative Action/Equal Opportunity
Institution.
----------------------------
For general information only contact me directly at: stefan@msu.edu
------------------
Stefan Wagener, PhD, CBSP
Office of Radiation, Chemical and Biological Safety
Michigan State University
C-126 Research Complex Engineering
East Lansing, MI 48824-1326
Email: stefan@pilot.msu.edu
Phone: (517) 355-6503
Fax: (517) 353-4871
=========================================================================
Date: Wed, 19 May 1999 15:48:42 -0400
Reply-To: "eagleson@"
Sender: A Biosafety Discussion List
From: Eagleson Institute
Subject: ABSA/Eagleson Inquiry
MIME-Version: 1.0
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Dear Biosafty Members,
The third ABSA/Eagleson Spring Seminar Series took place two weeks ago in
Atlanta and was once again a big success! One of the courses we offered was
Emergency Response to Bioterrorism. Because it was so well received, we
are considering offering this class on the West Coast in November.
However, we need help deciding whether or not the interest is strong
enough, as we realize that there are many other training programs taking
place this fall and budgets are limited.
If you are interested in attending a two-day program on Bioterrorism on a
Friday and Saturday in November, possibly the 5th and 6th, please email me
at eagleson@ . The class will be held somewhere near the San
Francisco airport, making it convenient for people to attend from either
Southern California or the Northwest states. The cost will be $500 to
attend.
Thank you!
Mary Ann Sondrini
Eagleson Institute
P.O. Box 954
Sanford, ME 04073
(207) 490-1076
(207) 324-3869 Fax
eagleson@
=========================================================================
Date: Wed, 19 May 1999 16:37:17 -0500
Reply-To: HawkinsL@omrf.ouhsc.edu
Sender: A Biosafety Discussion List
From: Larry Hawkins
Organization: Oklahoma Medical Research Foundation
Subject: Vaccinia Virus
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Dear Biosafety People,
In the near future two of our scientist, who have worked with
vaccina at other institutions, will begin work here using
Vaccinia virus for gene expression vectors at OMRF.
(Neither were much help in establishing safety guidelines for this.)
Vaccinia is designated as a BSL2, which we can handle. A
risk assessment has been done on the procedures and high
risk area have been identified, ie sonification. All waste will
be treated as biomedical waste. All work areas are to be
decontaminated at the end of the procedure (that will be the
day). The work area will have limited access during the
procedures. All work will be done in a biosafety cabinet.
The stock solution of virus will contain 1-10 x 10*6
viral particles. The final extract will contain between
200-2000/ml.
Most of us older people have received the vaccination in our
youth but many of the younger employees have never been
vaccinated. I have contacted the CDC for the vaccine and have
been told we can not receive the vaccine because there is no
hyperimmune serum for vaccination reactions. Do any of
you know where I can find some??? Do we really need to
vaccinate the young ones?
If there are any pitfalls I have over looked please contact me
directly and let me know what to expect. Thanks to all of you
for your help in this matter.
Lawrence J. Hawkins
OMRF
825 NE 13th
Oklahoma City, OK 73104
Voice: 405.271.7266
Fax: 405.271.7012
E-mail: Larry-Hawkins@omrf.ouhsc.edu
=========================================================================
Date: Thu, 20 May 1999 16:53:51 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: performance plans
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A plea to those of you who have had to implement performance plans with
measurable objectives, for your positions as biosafety officers, IH or other
related fields. I would greatly appreciate if you could share with me what
types of objectives you have written, how they have been weighted and how
they have been measured
Please reply directly to me at therese.stinnett@uchsc.edu
Thanks in advance
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
=========================================================================
Date: Sat, 22 May 1999 21:46:26 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Richard W. Gilpin, Ph.D., RBP, CBSP"
Organization: Johns Hopkins University & Hospital
Subject: Last Call Johns Hopkins Biosafety Course June 14-18 1999
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There is still room for about eight more participants in the twentieth
edition of the Johns Hopkins University Control of Biohazards in the
Research Laboratory Course. If the HTML will not open on your computer,
give me a call and we will fax you a copy.
Thanks, Richard
--
Richard W. Gilpin, Ph.D., RBP, CBSP
Biosafety Officer
Assistant Professor of Medicine (SOM)
Assistant Professor of Environmental Health Sciences (SHPH)
Biosafety Division
Department of Health, Safety & Environment
Johns Hopkins Institutions
2024 East Monument Street, Room 2-700
Baltimore MD 21205-2223
Phone (410) 955-5918
Fax (410) 955-5929
Internet Email: gilpin@welch.jhu.edu
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Biocontrol Brochure 1999
Control
of BIOHAZARDS=
in the Research
Laboratory
June 14-18, 1999
Presented by
The Biosafety Division, Department of Health,
Safety and Environment
The Center for Occupational and Environmental
Health
The Educational Resource Center In Occupationa=
l
Safety and Health
The Johns Hopkins University
Baltimore, Maryland 21205
Course Description
The Control of Biohazards in the Research Labo=
ratory
is designed to provide instruction on the recognition and control of bioh=
azards
including infectious agents, oncogenic viruses, recombinant DNA, chemical
carcinogens and other toxic agents. Instruction will be provided in facil=
ity
design, in the practices and procedures of biohazard control, biosafety
regulations, and in the organization, planning and implementation of a
biosafety program.
Who Should Attend:
The course is directed to biosafety officers,
safety officers, industrial hygienists, directors of health and safety
programs, biosafety committee chairs and members, occupational health per=
sonnel,
clinical and biomedical laboratory supervisors, bench scientists and tech=
nicians,
architects and facility engineers. The course is designed to provide basi=
c
information to participants with or without prior biosafety experience.
Course Features:
This four and one half day course consists of
lectures, a laboratory exercise and opportunities for informal discussion=
s
with course faculty, for review of pertinent audiovisual materials and
for examination of biosafety equipment and devices. Theoretical backgroun=
d
and basic principles will be covered.
Subject Areas:
An overview of cell biology and host-parasit=
e
relationships
Hazard potential of infectious agents, recomb=
inant
DNA and
Oncogenic viruses
Dissemination of contaminants
Equipment designed for safety
Containment concepts: primary and secondary b=
arriers
Laboratory practices, hygiene, cGLP
Biosafety guidelines
Recombinant DNA
Standard precautions and bloodborne pathogens=
Safe handling and housing of laboratory anima=
ls
Sterilization and disinfection
Emerging Diseases
Tuberculosis overview
Emergency procedures
Federal regulations involving laboratory safe=
ty
Packaging and shipment of biological material=
s
Medical waste handling and disposal
General Information
Dates: June 14-18, 1999
Place: Omni Inner Harbor Hotel
101 West Fayette Street
Baltimore, MD 21201
Registration: 8:00-8:30 a.m., Mon=
day,
June 14, 1999
Fee: $1475 per person to include
registration, continental breakfasts, refreshments, 2 lunches and a banqu=
et.
All fees are payable in advance. A letter confirming enrollment will be
sent to each registrant. Foreign payments must be made in a draft on a
U.S. bank. Only one-half of the fee will be returned if withdrawal is mad=
e
after May 14, 1999.
Course Credits: This program qual=
ifies
for 3 Continuing Education Credits from the Johns Hopkins University.&nbs=
p;
This course also qualifies for ABIH Certification Maintenance Points, BCS=
P
Continuance of Certification Points, and AAHP Continuing Education Credit=
s.
Course Manual: A course manual wi=
ll
provide lecture outlines, data tables and graphics used in the lectures,
copies of applicable standards and regulations, and other supporting mate=
rials.
Social Functions: A complimentary
banquet for registrants will be held on Thursday will be held on Thursday=
,
June 17. Guests are invited for a charge of $ 35.00.
Lodging: Accommodations have been
reserved at the Omni Inner Harbor Hotel. Rate: $159 Single or Double -
guaranteed until May 14, 1999.
Course Directors
Byron S. Tepper, Ph.D., CSP, CBSP is th=
e
former Director of the Office of Safety and Environmental Health of The
Johns Hopkins University and The Johns Hopkins Hospital. He has held acad=
emic
appointments in the Departments of Pathobiology and Environmental Health
Sciences in The Johns Hopkins University School of Hygiene and Public Hea=
lth.
He is currently an Assistant Professor of Occupational Medicine, The John=
s
Hopkins University School of Medicine. He is a Fellow of the American Aca=
demy
of Microbiology. Dr. Tepper is a microbiologist who entered the field of
biosafety after 15 years of research on leprosy and other mycobacterial
diseases. He is a Certified Safety Professional (CSP) and a Certified Bio=
logical
Safety Professional (CBSP) with more than 25 years experience in biosafet=
y,
occupational safety and environmental health. He developed and has contin=
uously
directed the course "Control of Biohazards in the Research Laboratory"
which has been presented at Johns Hopkins since 1979. He is a charter mem=
ber
and a Past-President of the American Biological Safety Association (ABSA)
and currently chair of the ABSA Biological Safety Professional Certificat=
ion
Committee. He is past president of the ABSA Chesapeake Area Chapter and
the Campus Safety Association (CSA). In 1998, Dr. Tepper was presented
the American Biological Safety Association=92s Arnold G. Wedum Distinguis=
hed
Achievement Award. Dr. Tepper is a recognized consultant in biosafety,
laboratory safety, laboratory design and hospital safety. He is currently
the CEO of BioControl, Inc., environmental health and safety consultants.=
Richard W. Gilpin, Ph.D., RBP, CBSP, is
the Director of the Biosafety Division, Department of Health, Safety and
Environment at The Johns Hopkins University and The Johns Hopkins Hospita=
l.
He is an Assistant Professor of Medicine at The Johns Hopkins University
School of Medicine and an Assistant Professor of Environmental Health Sci=
ence
at The Johns Hopkins University School of Hygiene and Public Health. Dr.
Gilpin is a basic/clinical/industrial research microbiologist with more
than 28 years experience in research, product development and environment=
al
health. He joined Hopkins eleven years ago after managing a department
of research and development for Becton Dickinson and Company. Dr. Gilpin
has directed microbiology courses for second year medical students, gradu=
ate
students, and pharmaceutical company marketing personnel. He is a Registe=
red
Biological Safety Professional, a Certified Biological Safety Professiona=
l,
and Councilor (Officer) of the American Biological Safety Association.
He is a past president of the Chesapeake Area Biological Safety Associati=
on
and a 32 year member of the American Society for Microbiology (Certified
in Biological Safety Microbiology by the American Academy of Microbiology=
).
Dr Gilpin is also a member of the American Society of Safety Engineers
and the American Academy for the Advancement of Science. He is a recogniz=
ed
expert on quantitative testing of legionella species in warm water contai=
ning
mechanical equipment. His consulting in environmental microbiology includ=
es
environmental microbiology, indoor air quality, and laboratory and hospit=
al
safety.
For Further Information Contact
Dr. Byron S. Tepper
Phone: (410) 828-6330
Fax: (410) 828-6331
tepper@
Dr. Richard W Gilpin
Phone: (410) 955-5918
Fax: (410) 955-5929
gilpin@welch.jhu.edu
Course Registration Form - June 1999=
Please mail remittance and completed regist=
ration
to:
Becky Vaeth, Registrar (410) 955-9213
Center for Occupational and Environmental Hea=
lth
The Johns Hopkins University
2024 E Monument Street, Suite 1-300
Baltimore, MD 21205-2223
Name:_________________________________________=
_________
Address:______________________________________=
__________
City:_____________________ State:_____ Zip Cod=
e:____________
Telephone Number: ( )_________________________=
_______
Fax ( )_______________________________________=
_____
Social Security Number _______________________=
____________
Employer______________________________________=
_________
Make checks payable to: JHU Biohazards Cour=
se.
Check or purchase order must accompany registration form to guarantee enr=
ollment
in the course.
If you have special needs or dietary requir=
ements,
please let us know so that arrangements can be made to accommodate
you
- - - - - - - - - - - - - - - - - - - - - -
- - - - - - - - - - - - - - - - - - - - - - - -
Hotel Reservation Form
Please complete and mail to:
Omni Inner Harbor Hotel Telephone (800) THE-OM=
NI
101 W. Fayette Street Telephone (410) 752-110=
0
Baltimore, MD 21201 Fax (410) 625-9646=
I am enrolled in the Biohazards Course present=
ed
by the Johns Hopkins University, June 14-18, 1999. Please make the
following reservation for me.
Rates- $159 Single or Double, per night.
Single(s) Doubles for nights
Reservations rate guaranteed until May 14, 19=
99.
Name _________________________________________=
_______________
Address ___________________________________=
___________________
City State Zip Code ___ __________
Area Code/ Telephone Number ( ) ______________=
________________
Arrival Date Departure Date ___________=
__________
_
Please hold my reservation for late arrival __=
________
Reservations may be guaranteed for late arrival by one night=92s deposit.
Include check payable to the hotel or use major credit card.
AMEX________ VISA_________ MASTER CARD _______=
_____
Card #____________________________Exp. Date___=
_________________
Signature____________________________________
Date______________
--------------DF354DFBF87A7983E2F149E0--
=========================================================================
Date: Mon, 24 May 1999 10:21:25 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Andrew Cockburn
Subject: Hantavirus and rodent trapping
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Hi all,
I was just reviewing an ecology protocol that incorporated rodent trap and
release for population surveys. We have been recommending dust masks and
latex gloves since we know that we have hantavirus in the area. However, the
CDC recommends "Workers should wear protective clothing, including a
surgeon's gown or coveralls (preferably disposable), shoe covers, latex
gloves (two pairs), and a half-face respirator and goggles or powered
air-purifying respirator (equipped with high-efficiency particulate air
filters)."
We can make this a requirement for the project, but I suspect that we will
have 0% compliance in the field. I can't imagine anyone hiking around in the
mountains in that getup, or putting it all on every time they stop to empty
a trap.
Do you have a current policy on what precautions mammologists should use
when working with rodent traps in the field?
Andrew Cockburn, PhD
Director of Biological Safety
West Virginia University
Morgantown, WV 26506-9006
Telephone 304-293-7157
Email acockbur@wvu.edu
=========================================================================
Date: Mon, 24 May 1999 10:35:35 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Job Opportunity
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
>>>>
From: "Betsy Gilman"
Good Morning!
If you or someone you know is interested in a challenging laboratory safety
position, please consider applying for the following newly created position
at Boston University Medical Center. The position is primarily focused on
biosafety and chemical safety.
7190 - Health & Safety Specialist - Environmental Health and Safety Office
Assist the Manager in the daily operations of maintaining Laboratory Safety
programs including laboratory and biosafety inspections and follow-up,
preparing reports, assisting with lab safety training, maintaining
laboratory chemical inventories. Respond to complaints and concerns
including chemical spills. Assist with the development and distribution of
policies and procedures. Assist with environmental compliance projects.
Support Institutional Biosafety Committee and the Laboratory Safety
Committee. Bachelor's Degree in Chemistry, Biosafety, Safety, or
Industrial Hygiene with one to three years of relevant experience required.
Interested individuals may contact me directly (please do not respond to
the list!) at egilman@bu.edu. I would be happy to
provide additional information or answer questions. Resumes and cover
letters should be sent directly to the Boston University Medical Campus,
Office of Personnel, 715 Albany Street, 560, Boston, MA 02118 (Attn:
Nancy Kraybill). Additional information may also be found on the website
at bumc.bu.edu.
Thanks!
Betsy
Elizabeth A. Gilman, MS, RBP, SM (NRM), CBSP
Laboratory Safety Manager / Biological Safety Officer
Office of Environmental Health & Safety
Boston University Medical Center
617-638-8842
617-638-8822 fax
egilman@bu.edu
>1. Surgical Table should be lower than elbow height
>> a. This should avoid resting on the table with the arms
>>2. If you lower the table, so will the employee to get closer to their
>>work. So I would recommend the use of a large glass magnifier with
>>light for the staff members to look through for their procedures.
>> a. This should prevent them from hunching over.
>>3. Place the surgical procedure if possible at a slant
>> a. This should prevent them from hunching over.
>>4. Back should be supported
>>5. Feet should be flat on the floor
>>6. Wrists should remain neutral
>
>
>To Bliss, et al,
>I would suggest looking into doing the procedure while seated and exploring
>different ways the animal is positioned on the operating table. Magnifying
>lupes are available and are often used in eye surgery. These are worn on
>the head and can be raised or lowered by assistants.
>TTFN,
>Larry
>
>Larry J. Thompson, DVM PhD DABVT
>Certified Biological Safety Specialist
>Director of Biosafety
>College of Veterinary Medicine-Cornell University
>Upper Tower Road phone 607-253-3900
>Ithaca, NY 14853 fax 607-253-3943
>
>
>
>"The best way to escape from a problem is to solve it."
> Alan Saporta
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Thu, 10 Jun 1999 11:40:55 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Adrian Felts
Subject: shorts in the lab
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hello Biosafety List,
I run the safety program at a BSL 2 facility. We have a "no shorts"
policy here for "safety reasons" (this policy was instituted by a
non-science administrator prior to my appointment as safety officer). Is
this rule necessary? I haven't found any specific mention of pants/shorts
apparel in any of the safety books that I have (Prudent Practices,
Biosafey in the laboratory and Laboratory Safety: Principles and
Practices), and have been asked by many of our employees to look into
having the rule rescinded.
We do have an animal facility (mice and rabbits) and only one of our
researchers uses organic solvents. The use of concentrated acids is
occasional (e.g. HCl).
I would like to rescind this rule and suggest that workers who wear shorts
to work also wear a lab coat (lab coats are worn anyway).
Any advice is greatly appreciated.
Thanks in advance.
Adrian Felts
Gamma-A Technologies
=========================================================================
Date: Thu, 10 Jun 1999 14:20:38 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: sue pedrick
Subject: Re: Animal surgery and ergonomic issues
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
If the table were lowered to below-elbow height, would they then be able to
sit in an "operating chair"? I once worked where a physician (who had
polio and walked with leg braces and crutches) was able to perform eye
surgery while sitting in something we called a "captain's chair", "bosun's
(sp?) chair" or "operating chair" (can't remember the name). It was like
sitting backwards on a rolling stool with a padded support rail which
encircled his torso and allowed him to lean his chest against it while
seated. (It differs from just a regular rolling stool used by plastic
surgeons, etc, in that it supports your arms and prevents you from falling
forward.
For what it's worth! Sue
At 02:06 PM 6/9/99 -0400, you wrote:
>Today I was asked by the veterinary staff that they would like ergonomic
>evaluations completed for the surgical procedures.
>
>The work they do and the set-up they use has forced them to lean forward to
>complete the task. This could possibly lead to shoulder, back and neck
>problems.
>
>I have a few recommendations - but I was wondering if anyone has run into
>the same issue and if anyone could provide anything besides the list I have
>come up with below.
>
>1. Surgical Table should be lower than elbow height
> a. This should avoid resting on the table with the arms
>2. If you lower the table, so will the employee to get closer to their
>work. So I would recommend the use of a large glass magnifier with
>light for the staff members to look through for their procedures.
> a. This should prevent them from hunching over.
>3. Place the surgical procedure if possible at a slant
> a. This should prevent them from hunching over.
>4. Back should be supported
>5. Feet should be flat on the floor
>6. Wrists should remain neutral
>
>
>Anything else?
>
>Thanks for your time.
>Bliss M. Schlank
>Biosafety Specialist
>Zeneca Pharmaceuticals
>1800 Concord Pike
>Wilmington DE 19850-5437
>302.886.2185
>Fax: 302.886.2909
>bliss.schlank@phwilm.
>
Sue Pedrick, RN, COHN-S
Clemson University
Occupational Health Nurse/Lecturer
101 Edwards Hall
Clemson, SC 29634-1702
Phone: (864) 656-5529
FAX: (864) 656-7694
=========================================================================
Date: Thu, 10 Jun 1999 12:13:16 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Elizabeth Smith
Subject: Re: shorts in the lab
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
I am currently addressing this issue in our chemistry labs.
As the safety manager, I oppose wearing shorts in a work area
which involves the use of chemicals which pose an immediate
hazard upon exposure to the skin. (Personally, I oppose wearing
shorts in a chemistry lab, period.)
The use of acetone, ethanol, and many inorganic salts (aluminum
hydroxide, sodium phosphate, saline, etc.) would not constitute
an "immediate hazard".
Concentrated acids (hydrochloric, nitric, sulfuric, etc.) and
stong bases (sodium/potassium hydroxide, etc.) all pose an
immediate hazard. If you get the stuff on your bare skin, it
will cause damage by the time you get to an emergency shower.
The use of animals in the facility leads me to ask what the real
hazard posed to bare legs would be from the presence of the mice
& rabbits. I think you would be more likely to require people
to wear long-sleeved shirts/lab coats to prevent miscellaneous
scratches while handling them.
Some points to consider regarding shorts & dress codes:
1. I have heard from the men in the lab: "If I can't wear
shorts, then the women shouldn't be able to wear short skirts."
I certainly see their point.
2. There are some religious groups which oppose women wearing
pants. This is not an issue I am aware of at our facility, but
on general principles, I am loathe to forbid wearing
skirts/dresses.
3. As a woman, I avoid wearing skirts that came above my knees
into a chemistry lab to work. And acids or organic solvents +
nylon stockings? No thanks.
4. If you accept shorts, how short is too short?
5. Are shorts/short skirts o.k. if a lab coat is worn over
them?
So, perhaps the rule should state that clothing should be at
least so long (e.g., below the knees), and not differentiate
what kind of clothing. Afterall, is there really any difference
between shorts that come to your knees and a dress that only
comes half way down your thighs?
Elizabeth
===
Elizabeth Smith
Environmental, Health & Safety
BioPort Corporation
3500 N. Martin L. King Blvd.
Lansing, MI 48906
517-327-1590
_________________________________________________________
Do You Yahoo!?
Get your free @ address at
=========================================================================
Date: Thu, 10 Jun 1999 12:17:50 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Wolz, Sarah"
Subject: Re: shorts in the lab
MIME-Version: 1.0
Content-Type: text/plain
We had quite a to do about this issue some years ago. Does one then also
prohibit the wearing of skirts?
The current policy is to "protect your arms, legs and feet when working with
hazardous materials or near someone who is working with hazardous
materials." We always do a summer memo reminding people about proper lab
attire for the tasks they are doing. I agree that a microbiologist
streaking plates can safely protect himself with a lab coat, but a molecular
biologist working on chloroform/phenol extractions might want to more fully
protect his legs. Chemists working with stronger corrosives are supplied
with chemically resistant aprons.
We hit hard the wearing of safety glasses, gloves, lab coats, jewelry, loose
hair, and adequately protective shoes (all parts of the Lab Standard). We
try to leave the fashion choices and judgement about leg coverings to the
discretion of the employees. We can only hope and remind people to use good
judgement.
Sarah Wolz
PathoGenesis Corp.
> ----------
> From: Adrian Felts[SMTP:afelts@OSF1.GMU.EDU]
> Reply To: A Biosafety Discussion List
> Sent: Thursday, June 10, 1999 8:40 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: shorts in the lab
>
> Hello Biosafety List,
>
> I run the safety program at a BSL 2 facility. We have a "no shorts"
> policy here for "safety reasons" (this policy was instituted by a
> non-science administrator prior to my appointment as safety officer). Is
> this rule necessary? I haven't found any specific mention of pants/shorts
> apparel in any of the safety books that I have (Prudent Practices,
> Biosafey in the laboratory and Laboratory Safety: Principles and
> Practices), and have been asked by many of our employees to look into
> having the rule rescinded.
> We do have an animal facility (mice and rabbits) and only one of our
> researchers uses organic solvents. The use of concentrated acids is
> occasional (e.g. HCl).
>
> I would like to rescind this rule and suggest that workers who wear shorts
> to work also wear a lab coat (lab coats are worn anyway).
>
> Any advice is greatly appreciated.
>
> Thanks in advance.
>
> Adrian Felts
> Gamma-A Technologies
>
=========================================================================
Date: Thu, 10 Jun 1999 15:19:05 -0400
Reply-To: rubockpa@UMDNJ.EDU
Sender: A Biosafety Discussion List
From: Paul Rubock
Organization: eohss-umdnj
Subject: [Fwd: work with human brain tissue]
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Date: Thu, 10 Jun 1999 15:16:47 -0400
From: Paul Rubock
Reply-To: rubockpa@umdnj.edu
Organization: eohss-umdnj
X-Mailer: Mozilla 4.06 [en] (Win95; I)
MIME-Version: 1.0
To: biosafty@mitva.mit.edu
Subject: work with human brain tissue
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A colleague and I were discussing safety issues for researchers handling
human brain tissue. It was my belief that unless there was reason to
believe that the material came from someone with a known or supected
CJD-type disease the approach would be to treat it as any other material
covered by the Bloodborne Pathogens Standard. Would anything beyond the
BPS application be called for in this instance?
Thank you
--------------736765842AD1F198FAC9DCFB--
=========================================================================
Date: Thu, 10 Jun 1999 17:46:46 +0000
Reply-To: gmerkle@pop.wright.edu
Sender: A Biosafety Discussion List
From: Greg Merkle
Subject: Re: shorts in the lab
In-Reply-To:
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The question that you present is a common complaint heard by people
that work in laboratory areas during the summer months. The common
statements that are made are that they do not use anything "that
hazardous" in the lab, its too hot to wear long pants and of course
the "we have never had an accident". I need to ask you, do you or
would you allow these same people to wear sandals in a lab? Its the
same thing. Before seeking to recind a policy I would you suggest
you check with a legal expert on the interpretation of the need for
personal protective equipment and the general duty clause of the OSHA
standard. You need to know what your liability is before a policy
that makes good sense is recinded.
=========================================================================
Date: Mon, 14 Jun 1999 09:34:06 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Bernholc, Nicole M"
Subject: Re: shorts in the lab
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HOw is working with shorts different than working in dresses?
THere should be a policy for all. Shorts have to be discussed in the
context.
-----Original Message-----
From: Wolz, Sarah [mailto:swolz@]
Sent: Thursday, June 10, 1999 3:18 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: shorts in the lab
We had quite a to do about this issue some years ago. Does one then also
prohibit the wearing of skirts?
The current policy is to "protect your arms, legs and feet when working with
hazardous materials or near someone who is working with hazardous
materials." We always do a summer memo reminding people about proper lab
attire for the tasks they are doing. I agree that a microbiologist
streaking plates can safely protect himself with a lab coat, but a molecular
biologist working on chloroform/phenol extractions might want to more fully
protect his legs. Chemists working with stronger corrosives are supplied
with chemically resistant aprons.
We hit hard the wearing of safety glasses, gloves, lab coats, jewelry, loose
hair, and adequately protective shoes (all parts of the Lab Standard). We
try to leave the fashion choices and judgement about leg coverings to the
discretion of the employees. We can only hope and remind people to use good
judgement.
Sarah Wolz
PathoGenesis Corp.
> ----------
> From: Adrian Felts[SMTP:afelts@OSF1.GMU.EDU]
> Reply To: A Biosafety Discussion List
> Sent: Thursday, June 10, 1999 8:40 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: shorts in the lab
>
> Hello Biosafety List,
>
> I run the safety program at a BSL 2 facility. We have a "no shorts"
> policy here for "safety reasons" (this policy was instituted by a
> non-science administrator prior to my appointment as safety officer). Is
> this rule necessary? I haven't found any specific mention of pants/shorts
> apparel in any of the safety books that I have (Prudent Practices,
> Biosafey in the laboratory and Laboratory Safety: Principles and
> Practices), and have been asked by many of our employees to look into
> having the rule rescinded.
> We do have an animal facility (mice and rabbits) and only one of our
> researchers uses organic solvents. The use of concentrated acids is
> occasional (e.g. HCl).
>
> I would like to rescind this rule and suggest that workers who wear shorts
> to work also wear a lab coat (lab coats are worn anyway).
>
> Any advice is greatly appreciated.
>
> Thanks in advance.
>
> Adrian Felts
> Gamma-A Technologies
>
=========================================================================
Date: Mon, 14 Jun 1999 08:57:04 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: protocol review and SBIR grants
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I would appreciate feedback from the group on the handling of requests from
outside individuals or companies for IBC reviews of protocols. Also in that
vein, if your campus has SBIR grant recipients operating on your campus or
in a contract with an SBIR recipient (e.g. for use of animal facilities),
how do you/would you handle the IBC review? I know some of you look at
issues beyond the NIH Guidelines on rDNA, so please elaborate on that
impact. Do you charge a fee to "ouside" companies?
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
=========================================================================
Date: Mon, 14 Jun 1999 12:00:25 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "P. Moravek"
Subject: shorts vs. dresses or skirts
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Dear Biosafty-netters,
In my experience, knee-length (or longer) skirts and dresses afford ample
protection from spills in the laboratory.
The skirt hangs somewhat away from the body, rather like an umbrella,
protecting the legs below. When residual liquid drips from a skirt it hits the
floor and maybe the shoes. In shorts, the liquid drips from the shorts
directly to the skin of the upper leg, onto the skin of the lower leg.
Certainly a great deluge of liquid would not be repelled by a skirt, but neither
would pants be great protection in such a case. Skin-tight and/or short skirts
would be as useless as shorts.
The "mechanics" of a knee-length skirt (or dress) as protection from spills in
the lab is demonstrated daily by the use of lab coats. Like skirts, they hang
away from the body somewhat, and by doing so, protects the legs below. That is,
if they are buttoned shut.
Discussion could go on and on about different fabrics, styles, etc. on the issue
of skirts. In my opinion, if a suitably long skirt is worn with a lab coat, the
protection is good for most lab procedures. Those procedures that have specific
dangers should be addressed on a case by case basis.
There is a policy on this campus that knee-length (or longer) skirts or pants
are acceptable in the lab, and shorts are not. Also closed shoes are
acceptable, and sandals or bare feet are not.
Excessively LONG skirts would be a tripping hazard, and should not be worn in
the laboratory for that reason.
--Paula Moravek
Biological Safety Officer
Worcester Polytechnic Institute
Worcester, MA U.S.A.
=========================================================================
Date: Mon, 14 Jun 1999 14:14:44 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Barbara Ernisse
Subject: Job Opportunity - Boston
In-Reply-To:
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Hello,
After 2 very interesting and challenging years, I am resigning my position as
Research Safety Specialist at Children's Hospital in Boston. This opens up an
opportunity for someone else. The researchers, co-workers and local safety
network are great, the work is diverse and meaningful and I will miss it but...
If you are interested in the position, please respond directly to the Human
Resources contacts given. I am not involved in the hiring process and will be
gone after Monday.
JOB REFERENCE ID 99.18357
LOCATION: Boston, MA
JOB CATEGORY: Health & Medicine
JOB TYPE: Full Time
JOB DESCRIPTION:
Manages safety programs such as hazardous chemical waste,
facility safety inspections, industrial hygiene monitoring, and Biosafety.
Works collaboratively to develop and implement programs and mediate solutions to
safety and health problems. Excellent communications skills and an ability to
develop and conduct training and education programs. Requires BA in
environmental health or related field plus 2-3 years experience. ABSA
certification a plus.
If interested in applying for this position, please forward your resume,
indicating the reference ID via the Children's Hospital Website
(). If you prefer to fax, please do so at
(617)355-7884, or mail to:
Children's Hospital
Human Resources
300 Longwood Avenue
Boston, MA 02115
Thanks for all the safety information, entertainment, and support. Hopefully
I'll see you in St. Louis
Barb
=========================================================================
Date: Tue, 15 Jun 1999 08:50:14 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: OSHA Inspection
MIME-Version: 1.0
Content-Type: text/plain
Has anyone ever been inspected by OSHA? ...I was just curious if anyone
could supply me with examples of the questions they may ask around
Biosafety. I know we have a good program - but still the fear of the
unknown...
Have a great week!
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Tue, 15 Jun 1999 08:12:12 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Heather H. Gonsoulin"
Subject: CJD decon
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Along these lines, if tissue is suspected to be infected with CJD what =
is the latest recommendation for decontamination in regard to tissue =
processing? =20
Heather H. Gonsoulin, RRA
Occupational Health and Safety Officer
USL-NIRC
( 318-482-0306 (fax) 318-373-0057
hah8377@usl.edu
-----Original Message-----
From: Paul Rubock [SMTP:rubockpa@umdnj.edu]
Sent: Thursday, 10 June, 1999 2:17 PM
To: biosafty@mitva.mit.edu
Subject: work with human brain tissue
A colleague and I were discussing safety issues for researchers handling =
human brain tissue. It was my belief that unless there was reason to =
believe that the material came from someone with a known or supected =
CJD-type disease the approach would be to treat it as any other material =
covered by the Bloodborne Pathogens Standard. Would anything beyond the =
BPS application be called for in this instance?
Thank you
=========================================================================
Date: Tue, 15 Jun 1999 09:28:21 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Elise Franklin
Subject: OSHA Inspection -Reply
Mime-Version: 1.0
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Bliss,
OSHA would be most likely to focus on items that are covered under their =
regulations such as bloodborne pathogens and hazard communication.
Elise Franklin
Nabi-Rockville
=========================================================================
Date: Tue, 15 Jun 1999 09:49:14 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Ralph Stuart, University of Vermont"
Subject: New NIOSH CD-ROM (Free)
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
I was able to see some of the preview copies of this CD and can attest to
its technical value and ease of use. Address for complimentary copies are
at the bottom.
- Ralph
=================================
NIOSH introduced a new CD-ROM product, entitled the "NIOSH Pocket Guide to
Chemical Hazards and Other Databases, DHHS (NIOSH) Publication No. 99-115,"
at the 1999 AIHCE in Toronto last week. This CD-ROM contains a selection of
popular databases that are available on the NIOSH website. In addition, it
contains a closely related database from the Department of Transportation
(DOT) website. The following is a complete list of the databases:
. Immediately Dangerous to Life and Health Concentrations
. International Chemical Safety Cards (U.S. National Version)
. NIOSH Certified Equipment List as of March 31, 1999
. NIOSH Manual of Analytical Methods
. NIOSH Pocket Guide to Chemical Hazards
. Recommendations for Chemical Protective Clothing
. Specific Medical Tests Published for OSHA Regulated Substances
. Toxicologic Review of Selected Chemicals
. 1996 North American Emergency Response Guidebook (DOT)
The users can view these databases on their personal computers without
Internet access, so the CD-ROM essentially brings a part of the NIOSH
website to the users. With the exception of the Certified Equipment List
(CEL), all the databases can be viewed with a web browser (e.g., Netscape
Navigator 3 or higher, or Microsoft Internet Explorer 3 or higher) and the
Adobe Acrobat Reader plugin on any operating system. The CEL is based on
Microsoft Access and must be installed on a Windows 95, 98 or NT system.
Complimentary copies of the CD-ROM are available from the NIOSH Publications
Office:
E-Mail: pubstaft@
Phone: 1-800-35-NIOSH
Fax: 1-513-533-8573
=========================================================================
Date: Tue, 15 Jun 1999 11:20:58 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Edwin Jackson
Subject: Re: shorts vs. dresses or skirts
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One of our graduate students working in a laboratory droped a five pint =
bottle of 98% sulfuric acid. The acid splashed onto his levi's. Rather =
than hit the safety shower he went home and changed. He didn't have a =
single chemical burn. =20
It is certainly true that anyone working with large quantities of =
concentrated sulfuric acid should have had a splash suit, and he should =
have showered on the spot. However; having worked around H2SO4 I was =
really impressed by the protection that was provided by the normal =
clothing. I can assure you that if the young man had been in shorts and =
even if he hit the shower fast he would have had some serious tissue =
damage from this type of exposure.
Ed Jackson
email: edwin_jackson@byu.edu
Fax: (801)-378-5977
Phone (801) 378-5779
Address: 122 TOMH BYU
Provo, Utah 84602-0100
=========================================================================
Date: Tue, 15 Jun 1999 14:10:41 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Karen Ruhl
Subject: MSDS/Biolologicals/FDA
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Good Day:
Do I need to create a MSDS for a biological product (with ~2% sodium azide)
that is regulated by the FDA, or is the package insert sufficient?
Thanks.
Karen Ruhl
MGR. EH&S
Chugai Biopharmaceuticals
San Diego, CA
karenr@
=========================================================================
Date: Wed, 16 Jun 1999 07:30:26 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Elizabeth Smith
Subject: Re: MSDS/Biolologicals/FDA
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
karen,
you are not required to generate MSDS for items which are
regulated by other agencies (e.g., FDA, USDA, etc.).
29 CFR 1910.1200(b)(6)(vii) - the regulation about MSDS
specifically exempts pharmaceuticals sold in the form in which
they will be administered to the patient.
29 CFR 1910.1200(b)(6)(xii) - same regulation exempts biological
hazards.
We have a form letter for both cases that:
cites the applicable regulation,
refers the client to the product insert, which contains all of
the information we would put in an MSDS anyway, and
provides the phone number for our health & safety Office (me) if
they have further questions about this
Elizabeth
===
Elizabeth Smith
Environmental, Health & Safety
BioPort Corporation
3500 N. Martin L. King Blvd.
Lansing, MI 48906
517-327-1590
_________________________________________________________
Do You Yahoo!?
Get your free @ address at
=========================================================================
Date: Wed, 16 Jun 1999 11:02:34 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "DEANNA S. ROBBINS"
Subject: Re: shorts in the lab
Wearing shorts in the lab is always a problem-particularly when you have
summer students. If you think about it, some of the shorter skirts are
about the length of longer (?) shorts! I think the issue is making the
wearing of fully closed lab coats mandatory regardless of the procedure
being done. Enforcing this rule is another issue so I would do more than
"suggest" it! You need strong support from management on this one to make
it fly. It can be done! I have used free pizza as a lure to get folks in to
talk about this issue to make it more "palatable."
Deanna S. Robbins, Ph.D.
Dept. of Veterans Affairs Medical Center
10 N. Greene Street
Baltimore, MD 21202
-----Original Message-----
From: Adrian Felts [SMTP:afelts@OSF1.GMU.EDU]
Sent: Thursday, June 10, 1999 11:41 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: shorts in the lab
Hello Biosafety List,
I run the safety program at a BSL 2 facility. We have a "no shorts"
policy here for "safety reasons" (this policy was instituted by a
non-science administrator prior to my appointment as safety officer). Is
this rule necessary? I haven't found any specific mention of pants/shorts
apparel in any of the safety books that I have (Prudent Practices,
Biosafey in the laboratory and Laboratory Safety: Principles and
Practices), and have been asked by many of our employees to look into
having the rule rescinded.
We do have an animal facility (mice and rabbits) and only one of our
researchers uses organic solvents. The use of concentrated acids is
occasional (e.g. HCl).
I would like to rescind this rule and suggest that workers who wear shorts
to work also wear a lab coat (lab coats are worn anyway).
Any advice is greatly appreciated.
Thanks in advance.
Adrian Felts
Gamma-A Technologies
=========================================================================
Date: Wed, 16 Jun 1999 11:05:02 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "DEANNA S. ROBBINS"
Subject: Re: shorts in the lab
Sorry, I think it is easier to take off a lab coat with acid or other nasty stuff on it than removing a dress, shirt or pants.
Deanna S. Robbins, Ph.D.
Dept. of Veterans Affairs Medical Center
10 N. Greene Street
Baltimore, MD 21202
-----Original Message-----
From: Elizabeth Smith [SMTP:safety_queen@]
Sent: Thursday, June 10, 1999 3:13 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: shorts in the lab
I am currently addressing this issue in our chemistry labs.
As the safety manager, I oppose wearing shorts in a work area
which involves the use of chemicals which pose an immediate
hazard upon exposure to the skin. (Personally, I oppose wearing
shorts in a chemistry lab, period.)
The use of acetone, ethanol, and many inorganic salts (aluminum
hydroxide, sodium phosphate, saline, etc.) would not constitute
an "immediate hazard".
Concentrated acids (hydrochloric, nitric, sulfuric, etc.) and
stong bases (sodium/potassium hydroxide, etc.) all pose an
immediate hazard. If you get the stuff on your bare skin, it
will cause damage by the time you get to an emergency shower.
The use of animals in the facility leads me to ask what the real
hazard posed to bare legs would be from the presence of the mice
& rabbits. I think you would be more likely to require people
to wear long-sleeved shirts/lab coats to prevent miscellaneous
scratches while handling them.
Some points to consider regarding shorts & dress codes:
1. I have heard from the men in the lab: "If I can't wear
shorts, then the women shouldn't be able to wear short skirts."
I certainly see their point.
2. There are some religious groups which oppose women wearing
pants. This is not an issue I am aware of at our facility, but
on general principles, I am loathe to forbid wearing
skirts/dresses.
3. As a woman, I avoid wearing skirts that came above my knees
into a chemistry lab to work. And acids or organic solvents +
nylon stockings? No thanks.
4. If you accept shorts, how short is too short?
5. Are shorts/short skirts o.k. if a lab coat is worn over
them?
So, perhaps the rule should state that clothing should be at
least so long (e.g., below the knees), and not differentiate
what kind of clothing. Afterall, is there really any difference
between shorts that come to your knees and a dress that only
comes half way down your thighs?
Elizabeth
===
Elizabeth Smith
Environmental, Health & Safety
BioPort Corporation
3500 N. Martin L. King Blvd.
Lansing, MI 48906
517-327-1590
_________________________________________________________
Do You Yahoo!?
Get your free @ address at
=========================================================================
Date: Thu, 17 Jun 1999 14:14:19 -0400
Reply-To: rubockpa@UMDNJ.EDU
Sender: A Biosafety Discussion List
From: Paul Rubock
Organization: eohss-umdnj
Subject: HIV Genetic Manipulations
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Hello,
An investigator at my institution will be propagating full-length HIV
genomes (the DNA-transcript, Iassume) in a plasmid in E.coli. Then,
they will insert variants of the env gene into the HIV genome. This
will be followed by infection of Hela cells resulting in the production
HIV virions that will be used to assess phenotypic differences. Concern
focuses on the safety precautions during the molecular biology phases of
the work ('cutting and pasting' DNA, running and manipulating gels,
plasmid prep procedures), particularly those that can not be contained
in a BSC.
My thoughts are to use BL-3 work practices for all activities with
intact virus and that BL-2 barrier precautions for the molecular bio
procedures where people are handling genetic material (as opposed to
whole virions) would be appropriate because we're still focusing on
preventing percutaneous and mucous membrane exposure. Does this seem
appropriate??
Also,are their any thoughts as to the degree of risk from, say, a
needlestick or other exposures to HIV genetic material ?? I wouldn't
imagine it can be any higher than exposure to the whole virus but how
much lower?
Thank you for your help.
Paul Rubock
UMDNJ
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Hello,
An investigator at my institution will be propagating full-length
HIV genomes (the DNA-transcript, Iassume) in a plasmid in E.coli.
Then, they will insert variants of the env gene into the HIV genome.
This will be followed by infection of Hela cells resulting in the production
HIV virions that will be used to assess phenotypic differences. Concern
focuses on the safety precautions during the molecular biology phases of
the work ('cutting and pasting' DNA, running and manipulating gels, plasmid
prep procedures), particularly those that can not be contained in a BSC.
My thoughts are to use BL-3 work practices for all activities with intact
virus and that BL-2 barrier precautions for the molecular bio procedures
where people are handling genetic material (as opposed to whole virions)
would be appropriate because we're still focusing on preventing percutaneous
and mucous membrane exposure. Does this seem appropriate??
Also,are their any thoughts as to the degree of risk from, say, a needlestick
or other exposures to HIV genetic material ?? I wouldn't imagine
it can be any higher than exposure to the whole virus but how much lower?
Thank you for your help.
Paul Rubock
UMDNJ
--------------1335DBDDA686553681400B5A--
=========================================================================
Date: Thu, 17 Jun 1999 12:20:44 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Catherine Thomas
Subject: Ergonomics Evaluations for Animal Care Facilities
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Greetings,
I have been asked to help conduct and ergonomic evaluation for the animal
caretakers in our animal care facility. I am looking for some guidance on
how best to approach this project. I am particularly interested in
experience that any of you might have with automating tasks such as cage
changing etc.. Also I am very interested in reading any studies which
might currently be published on this topic.
Any assistance in this area will be greatly appreciated. Please email me
directly.
Thank you.
Cathy
=========================================================================
Date: Fri, 18 Jun 1999 11:30:31 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Betty Kupskay
Subject: Lab acquired infections and videos showing aerosols
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Would any of you out in biosafety land like to share any documented incidents of
lab acquired infections? These references would be an invaluable educational
tool to use in my staff training sessions.
Also, if you know of any training videos that show the formation of aerosols
when carrying out routine micro work (ie. inoculating liquid cultures, streaking
plates, vortexing, etc.) would you please pass on their titles to me?
Thanks very much!
Betty Kupskay
Biosafety Specialist/Health Canada
Safety & Environmental Services
Canadian Science Centre for Human and Animal Health
1015 Arlington St., Suite A1010
Winnipeg, MB
R3E 3P6
ph: 204-789-2065
FAX: 204-789-2069
e:mail: betty_kupskay@hc-sc.gc.ca
=========================================================================
Date: Mon, 21 Jun 1999 08:21:14 +0100
Reply-To: doblhoff@edv2.boku.ac.at
Sender: A Biosafety Discussion List
From: DOBLHOFF-DIER OTTO
Organization: Universitaet fuer Bodenkultur Wien
Subject: Re: Lab acquired infections and videos showing aerosols
In-Reply-To:
MIME-Version: 1.0
Content-type: text/plain; charset=ISO-8859-1
Content-transfer-encoding: Quoted-printable
Dear Betty,
A valuable list of documented incidents compiled by Chris Collins
can be found on our homepage. This list is updated frequently and
will help you find corresponding publications to specific biological
agents.
Laboratory- and some other Occupationally-acquired Microbial
Diseases. A bibliography
Best wishes
Otto
Otto Doblhoff-Dier
Chairman Working Party Safety in Biotechnology of the European Federation =
Biotechnology
Inst. Appl. Microbiol, Univ. Agric.,
Nussdorfer L=E4nde 11, A-1190 Vienna, Austria, Europe
Tel: *43-1-36006-6204 Fax:*43-1-3697615
EMAIL: doblhoff@edv2.boku.ac.at
WWW:
=========================================================================
Date: Mon, 21 Jun 1999 10:54:55 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Bernholc, Nicole M"
Subject: Azides in pipes.
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
This is not exactly biosafety but I will ask anyway..
In doing decomissioning or repair do any of you look at azide residue in
pipes? The concern is explosion. I have looked around and there doesn't
seem to be any quick and dirty test to see if you have a problem. Do some
people do wipes and send it out?
Any discussion would be appreciated.
Nicole Bernholc cih
Brookhaven National Laboratory
Bld 129
Upton, NY 11973
516-344-2027
bernholc@
=========================================================================
Date: Mon, 21 Jun 1999 11:41:09 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Terri Hellman
Subject: Re: OSHA Inspection
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
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We had the state OSHA folks in last fall. The topics they focused on
included Bloodborn Pathogens, Chem Hygiene Training and Program,
Lockout/tagout, confined space and hazcom. Maybe it was the inspector but
he didn't like labs and concentrated on B&G issues.
Schlank Bliss BM wrote:
> Has anyone ever been inspected by OSHA? ...I was just curious if anyone
> could supply me with examples of the questions they may ask around
> Biosafety. I know we have a good program - but still the fear of the
> unknown...
> Have a great week!
> Bliss M. Schlank
> Biosafety Specialist
> AstraZeneca
> 1800 Concord Pike
> Wilmington DE 19850-5437
> 302.886.2185 Fax: 302.886.2909
> bliss.schlank@phwilm.
>
=========================================================================
Date: Sat, 19 Jun 1999 00:00:22 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: transgenic animals
Mime-Version: 1.0
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Hi all Biosafety Folks,
I have a question about the preparation of transgenic animals. We have a
vivarium tech who extracts eggs of mice (after surgical removal) by mouth
pipetting them with a pasteur pipette attached to a flexible tube with a
mouthpiece.("microinjection") She said this is commonly done in transgenic
or fertility work because of the level of control mouth pipetting provides.
However, the lab she is woking in is essentially a BSL2 lab with chemicals
and other hazards. So, I have a two fold question:
1. Is there another way besides a mouth pipette to extract eggs?
2. Or, do we have to move her to another area to ensure her protection from
the other work in the lab?
Any guidance would be appreciated.
Janice Flesher, MS
Principal Industrial Hygienist & Biosafety Officer
Environmental & Occupational Health & Safety Service
University of Medicine and Dentistry of NJ
97 Paterson Street #227
New Brunswick, NJ 08901
732-235-8497 phone
732-235-8499 fax
fleshejk@umdnj.edu
=========================================================================
Date: Tue, 22 Jun 1999 09:55:03 -0400
Reply-To: rubockpa@UMDNJ.EDU
Sender: A Biosafety Discussion List
From: Paul Rubock
Organization: eohss-umdnj
Subject: EHS: fines against colleges/universities
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Does anyone know of a source (web site, I assume) for year-by-year info.
on citations and fines levied by the EPA for RCRA violations at colleges
and universities? Ditto for OSHA actions against colleges and
universities.
Thank you,
Paul Rubock
=========================================================================
Date: Tue, 22 Jun 1999 11:36:19 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: vacuum line filters
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Hello to all,
I have been unable to locate a source for an in-line HEPA filter for a
vacuum line. Can anyone help me with a source and part number?
Janice
Janice Flesher, MS
Principal Industrial Hygienist / Biosafety Officer
Environmental & Occupational Health & Safety Service
University of Medicine and Dentistry of NJ
97 Paterson Street #227
New Brunswick, NJ 08901
732-235-8497 phone
732-235-8499 fax
fleshejk@umdnj.edu
=========================================================================
Date: Tue, 22 Jun 1999 11:59:00 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: transgenic animals
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
It is my understanding that this is common in in-vitro fertilisation
techniques as well. The problem, I have been told, is that mechanical
pipettes will damage the eggs being transfered. turbulence maybe?
There is no other way to do this.
Are the materials the technician is working with a concern or is it just
the lab space the tech is in?
If the lab space is the problem, move the technician.
If the problem is that nasty stuff is being pipetted, how about a filter?
There was a time when we could buy pippettes that came with cotton plugs to
prevent accidental ingestion.
Bob
>Hi all Biosafety Folks,
>
>I have a question about the preparation of transgenic animals. We have a
>vivarium tech who extracts eggs of mice (after surgical removal) by mouth
>pipetting them with a pasteur pipette attached to a flexible tube with a
>mouthpiece.("microinjection") She said this is commonly done in transgenic
>or fertility work because of the level of control mouth pipetting provides.
>
>However, the lab she is woking in is essentially a BSL2 lab with chemicals
>and other hazards. So, I have a two fold question:
>
>1. Is there another way besides a mouth pipette to extract eggs?
>2. Or, do we have to move her to another area to ensure her protection from
>the other work in the lab?
>
>Any guidance would be appreciated.
>Janice Flesher, MS
>Principal Industrial Hygienist & Biosafety Officer
>Environmental & Occupational Health & Safety Service
>University of Medicine and Dentistry of NJ
>97 Paterson Street #227
>New Brunswick, NJ 08901
>732-235-8497 phone
>732-235-8499 fax
>fleshejk@umdnj.edu
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Tue, 22 Jun 1999 12:00:57 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Petuch, Brian R."
Subject: Re: vacuum line filters
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Content-transfer-encoding: 7BIT
Gelman and Whatman manufacture a specific filter; contact Fisher Scientific.
Contact Pall or Millipore if you need a steel housing style.
> ----------
> From: Janice Flesher[SMTP:fleshejk@UMDNJ.EDU]
> Sent: Tuesday, June 22, 1999 11:36 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: vacuum line filters
>
> Hello to all,
>
> I have been unable to locate a source for an in-line HEPA filter for a
> vacuum line. Can anyone help me with a source and part number?
>
> Janice
>
>
> Janice Flesher, MS
> Principal Industrial Hygienist / Biosafety Officer
> Environmental & Occupational Health & Safety Service
> University of Medicine and Dentistry of NJ
> 97 Paterson Street #227
> New Brunswick, NJ 08901
> 732-235-8497 phone
> 732-235-8499 fax
> fleshejk@umdnj.edu
>
=========================================================================
Date: Tue, 22 Jun 1999 12:59:35 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: transgenic animals
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
She doesn't want to put things in her mouth. I wish everyone would be so
careful. Perhaps we could have her disinfect the mouthpiece as well as
moving her from the "dirty" lab.
At 11:59 AM 6/22/1999 -0500, you wrote:
>It is my understanding that this is common in in-vitro fertilisation
>techniques as well. The problem, I have been told, is that mechanical
>pipettes will damage the eggs being transfered. turbulence maybe?
>
>There is no other way to do this.
>
>Are the materials the technician is working with a concern or is it just
>the lab space the tech is in?
>
>If the lab space is the problem, move the technician.
>
>If the problem is that nasty stuff is being pipetted, how about a filter?
>There was a time when we could buy pippettes that came with cotton plugs to
>prevent accidental ingestion.
>
>Bob
>
>
>
>>Hi all Biosafety Folks,
>>
>>I have a question about the preparation of transgenic animals. We have a
>>vivarium tech who extracts eggs of mice (after surgical removal) by mouth
>>pipetting them with a pasteur pipette attached to a flexible tube with a
>>mouthpiece.("microinjection") She said this is commonly done in transgenic
>>or fertility work because of the level of control mouth pipetting provides.
>>
>>However, the lab she is woking in is essentially a BSL2 lab with chemicals
>>and other hazards. So, I have a two fold question:
>>
>>1. Is there another way besides a mouth pipette to extract eggs?
>>2. Or, do we have to move her to another area to ensure her protection from
>>the other work in the lab?
>>
>>Any guidance would be appreciated.
>>Janice Flesher, MS
>>Principal Industrial Hygienist & Biosafety Officer
>>Environmental & Occupational Health & Safety Service
>>University of Medicine and Dentistry of NJ
>>97 Paterson Street #227
>>New Brunswick, NJ 08901
>>732-235-8497 phone
>>732-235-8499 fax
>>fleshejk@umdnj.edu
>
>
> ________________________________________________
>__ / _______________________________________________
>_ \ / /Robert N. Latsch USSF State Referee 6 Environmental Safety
> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
> \ / U.S.A. RA Member Environmental Safety
>
>
Janice
=========================================================================
Date: Tue, 22 Jun 1999 13:04:36 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Paul Zel
Subject: Re: vacuum line filters
In-Reply-To:
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Janice: We routinely use a "Millipore Millex filter unit for vacuum
line protection". This is available with a hydrophobic 0.2 micron
size filter membrane. It's available through Fisher Scientific
(1998/99 catalog, page 570) Cat. # SLFG 050 10 ($73.00 for pack of
10) -- for latex tubing. Cat. # SLFG 850 10 is for silicone tubing
(same price). Good luck.
Paul Zel
Dir. EH&S
Memorial Sloan-Kettering Cancer Center
New York, N.Y. 10021
(212) 639-7849
zelp@
______________________________ Reply Separator _________________________________
Subject: vacuum line filters
Author: fleshejk (fleshejk@UMDNJ.EDU) at Internet
Date: 6/22/99 11:36 AM
Hello to all,
I have been unable to locate a source for an in-line HEPA filter for a
vacuum line. Can anyone help me with a source and part number?
Janice
Janice Flesher, MS
Principal Industrial Hygienist / Biosafety Officer
Environmental & Occupational Health & Safety Service
University of Medicine and Dentistry of NJ
97 Paterson Street #227
New Brunswick, NJ 08901
732-235-8497 phone
732-235-8499 fax
fleshejk@umdnj.edu
=========================================================================
Date: Tue, 22 Jun 1999 13:26:24 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph P. Kozlovac"
Subject: Re: vacuum line filters
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Try checking the Pall and Gelman filter catalogues.. We are currently
using Pall "Vacushield" filters for that purpose.
At 11:36 AM 6/22/99 -0400, you wrote:
>Hello to all,
>
>I have been unable to locate a source for an in-line HEPA filter for a
>vacuum line. Can anyone help me with a source and part number?
>
>Janice
>
>
>Janice Flesher, MS
>Principal Industrial Hygienist / Biosafety Officer
>Environmental & Occupational Health & Safety Service
>University of Medicine and Dentistry of NJ
>97 Paterson Street #227
>New Brunswick, NJ 08901
>732-235-8497 phone
>732-235-8499 fax
>fleshejk@umdnj.edu
______________________________________________________________________________
Biological Safety Officer
Safety and Environmental Protection Program
NCI - Frederick Cancer Research
and Development Center
(301)846-1451 fax: (301)846-6619
email: jkozlovac@mail.
______________________________________________________________________________
=========================================================================
Date: Tue, 22 Jun 1999 13:43:05 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: on-call
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
some time back an individual contacted us regarding a survey of on-call pay
and how various institutions handle on-call emergency response.....
I am asking for some colleagues: do you have only the professional, exempt
staff handling on call? if not, how do you handle on-call and call-back pay
or comp time for your non-exempt staff?
Please respond directly to me....
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
=========================================================================
Date: Wed, 23 Jun 1999 14:35:57 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Lauer
Subject: Re: transgenic animals
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
At 12:00 AM -0400 6/19/99, Janice Flesher wrote:
>Hi all Biosafety Folks,
>
>I have a question about the preparation of transgenic animals. We have a
>vivarium tech who extracts eggs of mice (after surgical removal) by mouth
>pipetting them with a pasteur pipette attached to a flexible tube with a
>mouthpiece.("microinjection") She said this is commonly done in transgenic
>or fertility work because of the level of control mouth pipetting provides.
>
>However, the lab she is woking in is essentially a BSL2 lab with chemicals
>and other hazards. So, I have a two fold question:
>
>1. Is there another way besides a mouth pipette to extract eggs?
>2. Or, do we have to move her to another area to ensure her protection from
>the other work in the lab?
Move the person to another area where there are no chemical or biological
hazards The problem with mouth pippetting is no only ingestion of
materials in the pipette but one needs to put his/her finger in his/her
mouth. If the fingers are contaminated than one will ingest this
contamination. Filtered pippettes will not solve this concern.
>
>Any guidance would be appreciated.
>Janice Flesher, MS
>Principal Industrial Hygienist & Biosafety Officer
>Environmental & Occupational Health & Safety Service
>University of Medicine and Dentistry of NJ
>97 Paterson Street #227
>New Brunswick, NJ 08901
>732-235-8497 phone
>732-235-8499 fax
>fleshejk@umdnj.edu
Jim Lauer
University of Minnesota
Environmental Health & Safety
W-158
626-5621
lauer001@maroon.tc.umn.edu
=========================================================================
Date: Thu, 24 Jun 1999 11:20:49 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Elise Franklin
Subject: New Laboratory Safety Web Site -Reply
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Hi Elise is out of the office until July 6. If you need immediate EHS =
assistance please contact Ed Delvalle in Boca at 561-989-5800 ext 5618 or =
Jan Cameron for workers compensation questions at ext. 5511.
Have a Great Holiday!
=========================================================================
Date: Tue, 29 Jun 1999 08:57:55 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Wolz, Sarah"
Subject: BL3 reconfiguration
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Dear List:
Due to changes in research focus, we are considering some configurational
changes to our BL3 laboratory. Our current laboratory is very similar to
the "BL3 suite", as detailed in the recent Design of BSL3 Laboratories
chapter (in the Anthology of Biosafety I, Perspectives on Laboratory Design
by Dr. Jonathon Richmond). We have separate gown-in and gown-out passages,
a large central lab with several smaller labs branching off, and a 2-way
autoclave for sterilizing materials out of the lab. Very nice facility.
We want to adapt the majority of the lab space to more of a BSL2+ facility,
for work with aerosolized antibiotics. However, we still need some small
space for a bit of residual, periodic TB work. We plan, at least for now,
to reconfigure the space and work practices to coincide more with the "BSL3
Laboratory with a BSL2 Laboratory as an Access Zone", using several of the
smaller labs as our BL3 areas, with the larger lab converted to BSL2.
Therefore, entry/exit to the BSL3 areas would be from the larger BSL2
central laboratory space, no longer with direct access to the gown-in and
gown-out areas.
So, the question that seems to be of the most concern to staff is how will
we now gown out of the smaller BL3 labs, without contaminating the BSL2
area. Any of you who have this type of lab set-up--I'd appreciate hearing
more about some of your SOPs for entry and exit, removal of waste, etc.
We've enjoyed such a wonderful, dedicated facility--but I know there are
other ways to do this type of work safely, with a somewhat modified
facility.
Thanks--
Sarah Wolz
EH&S
PathoGenesis Corp.
swolz@
=========================================================================
Date: Thu, 1 Jul 1999 09:40:00 -0400
Reply-To: "eagleson@"
Sender: A Biosafety Discussion List
From: Eagleson Institute
Subject: Fall ABSA/Eagleson Seminar - Emergency Response to Bioterrorism
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After receiving a positive response about the possibility of offering a course on bioterrorism on the west coast,
we would like to announce the Fall ABSA/Eagleson Seminar on "Emergency Response to Bioterrorism."
This two-day course is specifically designed to train and inform health and safety professionals, public health
officials, and law enforcement personnel about the real risk associated with biological agents. The course will
not only cover the most likely biological agents, but also their acquisition and delivery, legislation, emergency
response, decontamination and protection, as well as biosecurity. Exercises will focus on real-life examples,
including "anthrax hoaxes."
The instructors are Stefan Wagener, Ph.D., CBSP, Biosafety Officer, Michigan State University,
Robert Hawley, Ph.D. CBSP, Chief, Safety and Radiation Protection, USAMRIID and
John Ezzell, Ph.D., Chief, Special Pathogens Branch, Diagnostic Systems Division, USAMRIID
This seminar will take place on November 12-13, 1999 (Friday and Saturday) at the Embassy Suites Hotel in South San Francisco.
For more information, please email us at eagleson@ or call us at 207.490.1076.
Eagleson Institute
P.O. Box 954
Sanford, ME 04073
(207) 490-1076
(207) 324-3869 Fax
eagleson@
=========================================================================
Date: Thu, 1 Jul 1999 12:35:07 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Cheri Marcham
Subject: Bioterrorism Course/Recombinant DNA course
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After seeing the posting on the Fall ABSA/Eagleson Seminar on "Emergency
Response to Bioterrorism", which was also offered in the Spring along with
the course, "Biological Safety Issues in the Development and Use of
Recombinant DNA Gene Delivery Technologies", I contacted Eagleson to
see if they would be offered together again. (Since the seminars were in
the Spring, by the time I knew about them our travel budget was already
spent for the fiscal year and was unable to attend.) I was told that the
rDNA/Gene Therapy course would probably not be offered again because there
was a limited audience for such a course.
Would there be others out in Biosafty land that might also be interested in
encouraging Eagleson to repeat both courses together?????
Cheri Marcham, CIH, CSP, CHMM
The University of Oklahoma Health Sciences Center
Environmental Health and Safety Office
cheri-marcham@ouhsc.edu
(405) 271-3000
=========================================================================
Date: Thu, 1 Jul 1999 12:21:14 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: Re: Bioterrorism Course/Recombinant DNA course
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I second Cheri's motion. The only info I had for the Spring seminar was on
the BT and that is not an integral part of my duties. The bio safety issues
absolutely are--FDA has new Points to Consider on human somatic and gene
therapy, what are our institutions doing to incorporate those into someone's
area of responsibility? How are we interacting with our respective IRBs &
IBCs? How is training for both IRBs and IBCs in the safety issues and
internal inspection or audit being addressed? I personally feel I would
benefit from a seminar that looks at the cGLP and QA issues where FDA has
oversight responsibility, but I'm operating from the NIH Guidelines, not
the FDA regs.
And--our fiscal/academic year is starting now, so if I need to request
something, I need to know now.
=========================================================================
Date: Fri, 2 Jul 1999 07:49:19 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Testing for aerosols
MIME-Version: 1.0
Content-Type: text/plain
Good morning.
Research staff at my company will be using a TECAN robot to manipulate and
study cell membranes. This project may change to include human cell lines.
Has anyone done testing for aerosol generation/contamination on these robots
or similar biotechnology?
I was planning to complete a study using methods similar to the ones handed
out at the "Control of Biohazards in the Research Laboratory" course. Can
you just study the generation of aerosols using a fluorescent dye? or do
you need to use the bacteria?
Any guidance on measuring or quantifying and aerosol
generation/contamination study is greatly appreciated. This will be my
first!
Have a great Fourth!
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Fri, 2 Jul 1999 09:56:48 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Bernholc, Nicole M"
Subject: CONFERENCE: HAZARD CONTROL TECHNOLOGIES IN HEALTHCARE:
MIME-Version: 1.0
Content-Type: multipart/mixed; boundary="----_=_NextPart_000_01BEC492.BC8B9A4C"
This message is in MIME format. Since your mail reader does not understand
this format, some or all of this message may not be legible.
------_=_NextPart_000_01BEC492.BC8B9A4C
Content-Type: text/plain;
charset="iso-8859-1"
This may be of interest to some of you
From: Margie Herbers [mailto:mherbers@]
Sent: Tuesday, June 29, 1999 11:23 AM
To: healthcare@acgih-
Subject: Conference program
To: Infectious Agents Committee Members
In the attached file is an electronic copy of the program for the
conference. Hopefully, having an electronic version available to you will
help your efforts in promoting the conference. Thank you for your help!
Margie Herbers
Education Group Leader
------_=_NextPart_000_01BEC492.BC8B9A4C
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XxAAAAYAAwEAAQABEADU
------_=_NextPart_000_01BEC492.BC8B9A4C--
=========================================================================
Date: Tue, 6 Jul 1999 11:50:56 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Robin Newberry
Subject: The new CDC/NIH BMBL (4th Ed.)
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
I just got a copy of the 4th Ed. of the BMBL; I've not had time to
read it yet, but I've a couple of questions:
1) Are there substantial changes from the 3rd Ed.?
2) You used to be able to get free copies, now there is only info on
how to buy it from the GPO. Are free copies still available to
Universities and State Agencies?
Many TIA,
Robin
W. Robert Newberry, IV CIH, CHMM
Director, Environmental Health and Safety
Clemson University
wnewber@clemson.edu ehs@clemson.edu
################## NOTICE TO BULK E-MAILERS ###################
##### PLEASE READ THIS BEFORE BULK E-MAILING THIS ADDRESS #####
Pursuant to US Code, Title 47, Chapter 5, Subchapter II, p.227, any
and all non-solicited commercial E-mail sent to the address of the
sender of this message is subject to a download and archival fee in
the amount of $500.00 US. Anyone who sends unsolicited commercial
e-mail to this account will be charged a $500.00 US proofreading fee.
This is an official notification. Failure to abide by this may result
in civil and/or criminal legal action as allowed under United States
law. For a complete summary of this legislation see:
###############################################################
=========================================================================
Date: Tue, 6 Jul 1999 11:13:30 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: The new CDC/NIH BMBL (4th Ed.)
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Robin:
I suggest we all read it and digest the new edition as it is very helpful
and represents much hard work by the guest editors and Jonathan
Richmond and Bob McKinney. Our thanks goes out most sincerely to all who
helped get this edition out. We needed it badly!
Get it from the Web site or order it from US GOV. Printing Office.
FYI: The major changes are summarized on page viii and ix by the editors.
Joe Coggin, Jr. Ph.D.
RBP, CBSP
Professor and Chairman,
U. South Alabama, Coll. of Medicine
LMB,
Mobile, AL 36688
On Tue, 6 Jul 1999, Robin Newberry wrote:
> I just got a copy of the 4th Ed. of the BMBL; I've not had time to
> read it yet, but I've a couple of questions:
>
> 1) Are there substantial changes from the 3rd Ed.?
>
> 2) You used to be able to get free copies, now there is only info on
> how to buy it from the GPO. Are free copies still available to
> Universities and State Agencies?
>
> Many TIA,
>
> Robin
>
> W. Robert Newberry, IV CIH, CHMM
> Director, Environmental Health and Safety
> Clemson University
>
> wnewber@clemson.edu ehs@clemson.edu
>
>
> ################## NOTICE TO BULK E-MAILERS ###################
> ##### PLEASE READ THIS BEFORE BULK E-MAILING THIS ADDRESS #####
> Pursuant to US Code, Title 47, Chapter 5, Subchapter II, p.227, any
> and all non-solicited commercial E-mail sent to the address of the
> sender of this message is subject to a download and archival fee in
> the amount of $500.00 US. Anyone who sends unsolicited commercial
> e-mail to this account will be charged a $500.00 US proofreading fee.
> This is an official notification. Failure to abide by this may result
> in civil and/or criminal legal action as allowed under United States
> law. For a complete summary of this legislation see:
>
> ###############################################################
>
=========================================================================
Date: Tue, 6 Jul 1999 11:18:27 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: Re: The new CDC/NIH BMBL (4th Ed.)
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
What is the web site address where you can find the 4th edition? I have
only been able to find the 3rd edition on-line.
Julie A. Johnson, Ph.D.
Biosafety Officer
Environmental Health and Safety
Iowa State University
Ames, IA 50011
e-mail: jajohns@iastate.edu
phone: 515-294-7657
fax: 515-294-9357
web site:
-----Original Message-----
From: Joseph H. Coggin Jr. [mailto:jcoggin@JAGUAR1.USOUTHAL.EDU]
Sent: Tuesday, July 06, 1999 11:14 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: The new CDC/NIH BMBL (4th Ed.)
Robin:
I suggest we all read it and digest the new edition as it is very helpful
and represents much hard work by the guest editors and Jonathan
Richmond and Bob McKinney. Our thanks goes out most sincerely to all who
helped get this edition out. We needed it badly!
Get it from the Web site or order it from US GOV. Printing Office.
FYI: The major changes are summarized on page viii and ix by the editors.
Joe Coggin, Jr. Ph.D.
RBP, CBSP
Professor and Chairman,
U. South Alabama, Coll. of Medicine
LMB,
Mobile, AL 36688
On Tue, 6 Jul 1999, Robin Newberry wrote:
> I just got a copy of the 4th Ed. of the BMBL; I've not had time to
> read it yet, but I've a couple of questions:
>
> 1) Are there substantial changes from the 3rd Ed.?
>
> 2) You used to be able to get free copies, now there is only info on
> how to buy it from the GPO. Are free copies still available to
> Universities and State Agencies?
>
> Many TIA,
>
> Robin
>
> W. Robert Newberry, IV CIH, CHMM
> Director, Environmental Health and Safety
> Clemson University
>
> wnewber@clemson.edu ehs@clemson.edu
>
>
> ################## NOTICE TO BULK E-MAILERS ###################
> ##### PLEASE READ THIS BEFORE BULK E-MAILING THIS ADDRESS #####
> Pursuant to US Code, Title 47, Chapter 5, Subchapter II, p.227, any
> and all non-solicited commercial E-mail sent to the address of the
> sender of this message is subject to a download and archival fee in
> the amount of $500.00 US. Anyone who sends unsolicited commercial
> e-mail to this account will be charged a $500.00 US proofreading fee.
> This is an official notification. Failure to abide by this may result
> in civil and/or criminal legal action as allowed under United States
> law. For a complete summary of this legislation see:
>
> ###############################################################
>
=========================================================================
Date: Tue, 6 Jul 1999 11:26:54 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: The new CDC/NIH BMBL (4th Ed.)
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Julie:
On Tue, 6 Jul 1999, Johnson, Julie A. wrote:
> What is the web site address where you can find the 4th edition? I have
> only been able to find the 3rd edition on-line.
>
> Julie A. Johnson, Ph.D.
> Biosafety Officer
> Environmental Health and Safety
> Iowa State University
> Ames, IA 50011
> e-mail: jajohns@iastate.edu
> phone: 515-294-7657
> fax: 515-294-9357
> web site:
>
>
> -----Original Message-----
> From: Joseph H. Coggin Jr. [mailto:jcoggin@JAGUAR1.USOUTHAL.EDU]
> Sent: Tuesday, July 06, 1999 11:14 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Re: The new CDC/NIH BMBL (4th Ed.)
>
>
> Robin:
> I suggest we all read it and digest the new edition as it is very helpful
> and represents much hard work by the guest editors and Jonathan
> Richmond and Bob McKinney. Our thanks goes out most sincerely to all who
> helped get this edition out. We needed it badly!
>
> Get it from the Web site or order it from US GOV. Printing Office.
>
> FYI: The major changes are summarized on page viii and ix by the editors.
>
> Joe Coggin, Jr. Ph.D.
> RBP, CBSP
> Professor and Chairman,
> U. South Alabama, Coll. of Medicine
> LMB,
> Mobile, AL 36688
>
> On Tue, 6 Jul 1999, Robin Newberry wrote:
>
> > I just got a copy of the 4th Ed. of the BMBL; I've not had time to
> > read it yet, but I've a couple of questions:
> >
> > 1) Are there substantial changes from the 3rd Ed.?
> >
> > 2) You used to be able to get free copies, now there is only info on
> > how to buy it from the GPO. Are free copies still available to
> > Universities and State Agencies?
> >
> > Many TIA,
> >
> > Robin
> >
> > W. Robert Newberry, IV CIH, CHMM
> > Director, Environmental Health and Safety
> > Clemson University
> >
> > wnewber@clemson.edu ehs@clemson.edu
> >
> >
> > ################## NOTICE TO BULK E-MAILERS ###################
> > ##### PLEASE READ THIS BEFORE BULK E-MAILING THIS ADDRESS #####
> > Pursuant to US Code, Title 47, Chapter 5, Subchapter II, p.227, any
> > and all non-solicited commercial E-mail sent to the address of the
> > sender of this message is subject to a download and archival fee in
> > the amount of $500.00 US. Anyone who sends unsolicited commercial
> > e-mail to this account will be charged a $500.00 US proofreading fee.
> > This is an official notification. Failure to abide by this may result
> > in civil and/or criminal legal action as allowed under United States
> > law. For a complete summary of this legislation see:
> >
> > ###############################################################
> >
>
=========================================================================
Date: Tue, 6 Jul 1999 11:38:37 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: The new CDC/NIH BMBL (4th Ed.)
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Julie:
Web site Address:
From: Joe Coggin
On Tue, 6 Jul 1999, Johnson, Julie A. wrote:
> What is the web site address where you can find the 4th edition? I have
> only been able to find the 3rd edition on-line.
>
> Julie A. Johnson, Ph.D.
> Biosafety Officer
> Environmental Health and Safety
> Iowa State University
> Ames, IA 50011
> e-mail: jajohns@iastate.edu
> phone: 515-294-7657
> fax: 515-294-9357
> web site:
>
>
> -----Original Message-----
> From: Joseph H. Coggin Jr. [mailto:jcoggin@JAGUAR1.USOUTHAL.EDU]
> Sent: Tuesday, July 06, 1999 11:14 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Re: The new CDC/NIH BMBL (4th Ed.)
>
>
> Robin:
> I suggest we all read it and digest the new edition as it is very helpful
> and represents much hard work by the guest editors and Jonathan
> Richmond and Bob McKinney. Our thanks goes out most sincerely to all who
> helped get this edition out. We needed it badly!
>
> Get it from the Web site or order it from US GOV. Printing Office.
>
> FYI: The major changes are summarized on page viii and ix by the editors.
>
> Joe Coggin, Jr. Ph.D.
> RBP, CBSP
> Professor and Chairman,
> U. South Alabama, Coll. of Medicine
> LMB,
> Mobile, AL 36688
>
> On Tue, 6 Jul 1999, Robin Newberry wrote:
>
> > I just got a copy of the 4th Ed. of the BMBL; I've not had time to
> > read it yet, but I've a couple of questions:
> >
> > 1) Are there substantial changes from the 3rd Ed.?
> >
> > 2) You used to be able to get free copies, now there is only info on
> > how to buy it from the GPO. Are free copies still available to
> > Universities and State Agencies?
> >
> > Many TIA,
> >
> > Robin
> >
> > W. Robert Newberry, IV CIH, CHMM
> > Director, Environmental Health and Safety
> > Clemson University
> >
> > wnewber@clemson.edu ehs@clemson.edu
> >
> >
> > ################## NOTICE TO BULK E-MAILERS ###################
> > ##### PLEASE READ THIS BEFORE BULK E-MAILING THIS ADDRESS #####
> > Pursuant to US Code, Title 47, Chapter 5, Subchapter II, p.227, any
> > and all non-solicited commercial E-mail sent to the address of the
> > sender of this message is subject to a download and archival fee in
> > the amount of $500.00 US. Anyone who sends unsolicited commercial
> > e-mail to this account will be charged a $500.00 US proofreading fee.
> > This is an official notification. Failure to abide by this may result
> > in civil and/or criminal legal action as allowed under United States
> > law. For a complete summary of this legislation see:
> >
> > ###############################################################
> >
>
=========================================================================
Date: Tue, 6 Jul 1999 09:32:22 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Sandra Fry
Subject: CJD/ engineering controls
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; CHARSET=ISO-8859-1
Does anyone have information on engineering controls available for autopsy
suites handling CJD infected tissue? Aerosol generation is high and a
hospital is looking for alternatives to PAPR protection....
Thanks!
Sandra C. Fry
Biosafety Officer
BC Centre for Disease Control,
655 W. 12th Avenue
Vancouver, BC V5Z 4R4
phone: (604) 660 6167
pager: (604) 893 5737
fax: (604) 660 6073
=========================================================================
Date: Tue, 6 Jul 1999 13:19:26 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: fixed cells
MIME-Version: 1.0
Content-Type: text/plain
was wondering if anyone had a reference to "fixed" tissues, human cells,
viruses, etc. and the safety it offers to the user.
Is the agent that is fixed rendered non-infectious through this process?
Do you always use paraformaldehyde?
I would also like a actual procedure for this "fixing" process.
Thank you and I hope everyone enjoyed the fourth!
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Tue, 6 Jul 1999 13:19:29 EDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Re: CJD/ engineering controls
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Sandy:
There is a web site in the UK where one can obtain a guidance document from
the Advisory Committee on Dangerous Pathogens Spongiform Encephalopathy
Advisory Committee.
The most recent version I saw was 7 May, 1998. Keep in mind there are
diverging opinions on issues related to cjd and nv-cjd on both sides of the
big pond!
official-documents.co.uk/document/doh/spongifm/report.htm
You may also go to official-documents.co.uk and do a search.
Ed Krisiunas
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
ekrisiunas@
In a message dated 7/6/99 12:55:20 PM, sandra.fry@BCCDC.HNET.BC.CA writes:
=========================================================================
Date: Tue, 6 Jul 1999 15:48:36 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Pamela Hubley
Subject: Re: The new CDC/NIH BMBL (4th Ed.)
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Hi Joseph,
I find the 3rd edition posted on the site you listed below. Where should I
be looking?
Pamela Hubley, CIH, CSP
Millipore Corp.
Email: pamela_hubley@
"Joseph H. Coggin Jr." on 07/06/99 12:26:54
PM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Pamela Hubley/NA/Millipore)
Subject: Re: The new CDC/NIH BMBL (4th Ed.)
Julie:
On Tue, 6 Jul 1999, Johnson, Julie A. wrote:
> What is the web site address where you can find the 4th edition? I have
> only been able to find the 3rd edition on-line.
>
> Julie A. Johnson, Ph.D.
> Biosafety Officer
> Environmental Health and Safety
> Iowa State University
> Ames, IA 50011
> e-mail: jajohns@iastate.edu
> phone: 515-294-7657
> fax: 515-294-9357
> web site:
>
>
> -----Original Message-----
> From: Joseph H. Coggin Jr. [mailto:jcoggin@JAGUAR1.USOUTHAL.EDU]
> Sent: Tuesday, July 06, 1999 11:14 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Re: The new CDC/NIH BMBL (4th Ed.)
>
>
> Robin:
> I suggest we all read it and digest the new edition as it is very helpful
> and represents much hard work by the guest editors and Jonathan
> Richmond and Bob McKinney. Our thanks goes out most sincerely to all who
> helped get this edition out. We needed it badly!
>
> Get it from the Web site or order it from US GOV. Printing Office.
>
> FYI: The major changes are summarized on page viii and ix by the editors.
>
> Joe Coggin, Jr. Ph.D.
> RBP, CBSP
> Professor and Chairman,
> U. South Alabama, Coll. of Medicine
> LMB,
> Mobile, AL 36688
>
> On Tue, 6 Jul 1999, Robin Newberry wrote:
>
> > I just got a copy of the 4th Ed. of the BMBL; I've not had time to
> > read it yet, but I've a couple of questions:
> >
> > 1) Are there substantial changes from the 3rd Ed.?
> >
> > 2) You used to be able to get free copies, now there is only info on
> > how to buy it from the GPO. Are free copies still available to
> > Universities and State Agencies?
> >
> > Many TIA,
> >
> > Robin
> >
> > W. Robert Newberry, IV CIH, CHMM
> > Director, Environmental Health and Safety
> > Clemson University
> >
> > wnewber@clemson.edu ehs@clemson.edu
> >
> >
> > ################## NOTICE TO BULK E-MAILERS ###################
> > ##### PLEASE READ THIS BEFORE BULK E-MAILING THIS ADDRESS #####
> > Pursuant to US Code, Title 47, Chapter 5, Subchapter II, p.227, any
> > and all non-solicited commercial E-mail sent to the address of the
> > sender of this message is subject to a download and archival fee in
> > the amount of $500.00 US. Anyone who sends unsolicited commercial
> > e-mail to this account will be charged a $500.00 US proofreading fee.
> > This is an official notification. Failure to abide by this may result
> > in civil and/or criminal legal action as allowed under United States
> > law. For a complete summary of this legislation see:
> >
> > ###############################################################
> >
>
=========================================================================
Date: Tue, 6 Jul 1999 16:01:30 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stefan Wagener
Subject: BMBL is NOT on the web yet!
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Contrary to public believe, the new BMBL is not yet available at the CDC web
site.
Stefan
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Pamela Hubley
Sent: Tuesday, July 06, 1999 3:49 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: The new CDC/NIH BMBL (4th Ed.)
Hi Joseph,
I find the 3rd edition posted on the site you listed below. Where should I
be looking?
Pamela Hubley, CIH, CSP
Millipore Corp.
Email: pamela_hubley@
"Joseph H. Coggin Jr." on 07/06/99 12:26:54
PM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Pamela Hubley/NA/Millipore)
Subject: Re: The new CDC/NIH BMBL (4th Ed.)
Julie:
On Tue, 6 Jul 1999, Johnson, Julie A. wrote:
> What is the web site address where you can find the 4th edition? I have
> only been able to find the 3rd edition on-line.
>
> Julie A. Johnson, Ph.D.
> Biosafety Officer
> Environmental Health and Safety
> Iowa State University
> Ames, IA 50011
> e-mail: jajohns@iastate.edu
> phone: 515-294-7657
> fax: 515-294-9357
> web site:
>
>
> -----Original Message-----
> From: Joseph H. Coggin Jr. [mailto:jcoggin@JAGUAR1.USOUTHAL.EDU]
> Sent: Tuesday, July 06, 1999 11:14 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Re: The new CDC/NIH BMBL (4th Ed.)
>
>
> Robin:
> I suggest we all read it and digest the new edition as it is very helpful
> and represents much hard work by the guest editors and Jonathan
> Richmond and Bob McKinney. Our thanks goes out most sincerely to all who
> helped get this edition out. We needed it badly!
>
> Get it from the Web site or order it from US GOV. Printing Office.
>
> FYI: The major changes are summarized on page viii and ix by the editors.
>
> Joe Coggin, Jr. Ph.D.
> RBP, CBSP
> Professor and Chairman,
> U. South Alabama, Coll. of Medicine
> LMB,
> Mobile, AL 36688
>
> On Tue, 6 Jul 1999, Robin Newberry wrote:
>
> > I just got a copy of the 4th Ed. of the BMBL; I've not had time to
> > read it yet, but I've a couple of questions:
> >
> > 1) Are there substantial changes from the 3rd Ed.?
> >
> > 2) You used to be able to get free copies, now there is only info on
> > how to buy it from the GPO. Are free copies still available to
> > Universities and State Agencies?
> >
> > Many TIA,
> >
> > Robin
> >
> > W. Robert Newberry, IV CIH, CHMM
> > Director, Environmental Health and Safety
> > Clemson University
> >
> > wnewber@clemson.edu ehs@clemson.edu
> >
> >
> > ################## NOTICE TO BULK E-MAILERS ###################
> > ##### PLEASE READ THIS BEFORE BULK E-MAILING THIS ADDRESS #####
> > Pursuant to US Code, Title 47, Chapter 5, Subchapter II, p.227, any
> > and all non-solicited commercial E-mail sent to the address of the
> > sender of this message is subject to a download and archival fee in
> > the amount of $500.00 US. Anyone who sends unsolicited commercial
> > e-mail to this account will be charged a $500.00 US proofreading fee.
> > This is an official notification. Failure to abide by this may result
> > in civil and/or criminal legal action as allowed under United States
> > law. For a complete summary of this legislation see:
> >
> > ###############################################################
> >
>
=========================================================================
Date: Tue, 6 Jul 1999 15:13:59 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: Re: BMBL is NOT on the web yet!
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
But I got my complimentary copy in today's mail!
=========================================================================
Date: Tue, 6 Jul 1999 20:40:00 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jose Vazquez Perez
Subject: BSL-3 exhaust system
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Dear Biosafety Folks,
I am involved in the process of designing the upgrade of a lab from
level 2 to level 3 for use with Mycobacterium tuberculosis, and would
appreciate any ideas or suggestions in interpreting the following
passage from the BMBL (4th edition) on the issue of an exhaust air
ventilation system:
"The outside exhaust must be dispersed away from occupied areas and air
intakes, or the exhaust must be HEPA-filtered." (item 9, p. 34.)
Specifically, I am interested in suggestions as to what mechanisms we
can use to 'disperse exhaust air away from outside air intakes' of air
handling units, e.g., do we require a certain distance between them (if
so, how far? 25, 50, 100 feet), or do we require the contractor to
exhaust the air in the direction opposite to any air intakes?
Thank you again,
Jose Vazquez
Biosafety Coordinator
Environmental Health & Safety
(407) 823-6301
jvazquez@mail.ucf.edu
=========================================================================
Date: Wed, 7 Jul 1999 07:16:20 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: fixed cells
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Hi Bliss. I have experience with fixing lymphocyte cultures (from blood) with
Cornoy's fixative (a glacial acetic acid, methanol mixture). It does render the
cells and any microbial contaminants non-infectious - dead! Fixed cells can be
disposed of as non-hazardous material.
Schlank Bliss BM on 07/06/99 12:19:26 PM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: fixed cells
was wondering if anyone had a reference to "fixed" tissues, human cells,
viruses, etc. and the safety it offers to the user.
Is the agent that is fixed rendered non-infectious through this process?
Do you always use paraformaldehyde?
I would also like a actual procedure for this "fixing" process.
Thank you and I hope everyone enjoyed the fourth!
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Wed, 7 Jul 1999 08:47:47 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: CJD/ engineering controls
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Sandy,
You will have to look since I no longer have this information. But here is
what I remember.
In the 1980's NIOSH did a formaldehyde study at a mortician's school in
Cincinnati, Ohio. One device used to limit formaldehyde exposure was a set
of ventilation ducts that attached to the autopsy table.
A company south of Toledo, Ohio, picked up on the idea. They were
manufacturing an autopsy table with built in ventilation ducts that created
an air curtain between the investigators and the subject.
I couldn't sell it at the time because of the cost, around $2K.
That is all I remember. Hope this helps.
Bob
>Sandy:
>
>There is a web site in the UK where one can obtain a guidance document from
>the Advisory Committee on Dangerous Pathogens Spongiform Encephalopathy
>Advisory Committee.
>
>The most recent version I saw was 7 May, 1998. Keep in mind there are
>diverging opinions on issues related to cjd and nv-cjd on both sides of the
>big pond!
>
>official-documents.co.uk/document/doh/spongifm/report.htm
>
>You may also go to official-documents.co.uk and do a search.
>
>Ed Krisiunas
>INSCITE
>115 Lyons Road
>Burlington, Connecticut
>06013
>860-675-1217
>860-675-1311(fax)
>ekrisiunas@
>
>
>In a message dated 7/6/99 12:55:20 PM, sandra.fry@BCCDC.HNET.BC.CA writes:
>
>hospital is looking for alternatives to PAPR protection....
>
>Thanks!
>
>Sandra C. Fry
>Biosafety Officer
>BC Centre for Disease Control,
>655 W. 12th Avenue
>Vancouver, BC V5Z 4R4
>phone: (604) 660 6167
>pager: (604) 893 5737
>fax: (604) 660 6073
>
>>>
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Wed, 7 Jul 1999 08:53:56 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: fixed cells
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Just a thought,
It is my understanding that fixed means in formaldehyde.
Formaldehyde does not affet CJD btw.
While the mixture may no longer be a biohazard, I think that you may now
have a chemical hazard depending on the formula.
bob
>Hi Bliss. I have experience with fixing lymphocyte cultures (from blood) with
>Cornoy's fixative (a glacial acetic acid, methanol mixture). It does
>render the
>cells and any microbial contaminants non-infectious - dead! Fixed cells
>can be
>disposed of as non-hazardous material.
>
>
>
>
>Schlank Bliss BM on 07/06/99 12:19:26 PM
>
>Please respond to A Biosafety Discussion List
>
>To: BIOSAFTY@MITVMA.MIT.EDU
>cc: (bcc: Judy M. Pointer/MDACC)
>Subject: fixed cells
>
>
>
>
> was wondering if anyone had a reference to "fixed" tissues, human cells,
>viruses, etc. and the safety it offers to the user.
>
>Is the agent that is fixed rendered non-infectious through this process?
>
>Do you always use paraformaldehyde?
>
>I would also like a actual procedure for this "fixing" process.
>
>Thank you and I hope everyone enjoyed the fourth!
>
>Bliss M. Schlank
>Biosafety Specialist
>AstraZeneca
>1800 Concord Pike
>Wilmington DE 19850-5437
>302.886.2185 Fax: 302.886.2909
>bliss.schlank@phwilm.
>
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Wed, 7 Jul 1999 08:56:28 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: ABSA's Honor Awards
Mime-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 8bit
>Date: Tue, 06 Jul 1999 12:40:50 -0400
>From: benjamin fontes
>Subject: ABSA's Honor Awards
>To: rfink@MIT.EDU
>X-Mailer: Mozilla 4.07 [en] (WinNT; U)
>
>Please post the following announcement regarding the American Biological
>Safety Association's (ABSA) two prestigious Honor Awards on the Biosafty
>list. Information regarding both awards and a nomination form are
>provided below. Nominations can be made on-line through the ABSA web
>site at , or by mailing the nomination form directly to:
>ABSA, attention Lynn Harding, 1202 Allanson Road, Mundelein, IL
>60060-3808. The deadline for nomination of colleagues for the 1999
>Honor Awards is July 15, 1999. Thank you in advance for your
>assistance.
>
>
>NOMINATIONS INVITED FOR 1999 HONOR AWARDS
>
>
>The Council has asked the ABSA Awards Committee to seek nominations for
>the 1999 ABSA Honor Awards, to be presented at the 42nd Annual
>Biological Safety Conference in St. Louis, Missouri, October 17-20,
>1999. ABSA members are encouraged to nominate appropriate candidates.
>
>The two honor awards for which nominations are being sought are Arnold
>G. Wedum Distinguished Achievement Award and Everett Hanel, Jr.
>Presidential Award. Details about the awards criteria are included in
>the description for each award. All nominations must be submitted on
>the form that follows by July 15, 1999 to Lynn Harding Chair, ABSA
>Awards Committee, American Biological Safety Association, 1202 Allanson
>Road, Mundelein, IL 60060-3808. The nomination form will also be
>available on-line on the ABSA Home Page () after April 1,
>1999.
>
>The ABSA Awards Committee will review nominations for the two honor
>awards and will recommend candidates to the Council for its
>consideration.
>
>Arnold G. Wedum Distinguished Achievement Award
>
>The Arnold G. Wedum Distinguished Achievement Award is given to an
>individual for outstanding contributions to biological safety
>accomplished through teaching, research, service, or leadership.
>Candidates for the award must be members of ABSA. Members of the 1999
>Council, the 1999 Awards Committee and previous recipients of this award
>are not eligible for the 1999 Arnold G. Wedum Distinguished Achievement
>Award.
>
>Recipients: Byron S. Tepper, 1998
> Melvin W. First, 1997
> Richard H. Kruse, 1995
>
>Everett Hanel, Jr. Presidential Award
>
>The Everett Hanel, Jr. Presidential Award is given to an individual for
>outstanding contributions to ABSA by promoting the field of biological
>safety and fostering, by example, the high professional standards of the
>ABSA membership. Candidates for the award must be members of ABSA.
>Members of the 1999 Council, 1999 ABSA Awards Committee and recipients
>of the previous President's Award are not eligible for the 1999 Everett
>Hanel, Jr. Presidential Award.
>
>Recipients:
> Mary L. Cipriano, 1998 John M. Eagleson, Jr., 1991
> Mary Ann Sondrini, 1997 Donald Vesley, 1990
> A. Lynn Harding, 1996 Manuel S. Barbeito, 1989
> Jonathan Y. Richmond, 1995 Jerome P. Schmidt, 1988
> Diane O. Fleming, 1994 W. Emmett Barkley, 1987
> Jerry J. Tulis, 1993 Joseph R. Songer, 1986
> Max. D. Peters, 1992 Everett Hanel, Jr., 1985
>
>ABSA 1999 Awards Committee
>Members: Lynn Harding
> Manuel Barbeito
> Ben Fontes
> Barbara Fox Nellis
> Joe Van Houten
>
> ABSA HONOR AWARDS-1999 NOMINATION
>
>Recommended ABSA Honor Award:
>( Arnold G. Wedum Distinguished Achievement Award
>( Everett Hanel, Jr. Presidential Award
>
>Nominee:
>
>
>Full Name
>
>Title/Organization
>
>Address
>
>Telephone ( ) Fax ( )
>
>Contributions and Achievements: Describe outstanding contributions and
>distinguished achievements which make nominee worthy of the designated
>ABSA Honor Award for 1999, fully considering the awards description.
>Attach additional sheets as necessary.
>
>
>Nominating Member:
>
>Full Name
>
>Title/Organization
>
>Address
>
>Telephone ( ) Fax ( )
>
>ABSA Membership Date
>
>Signature Date
>
>
>ABSA National Office:
>Confirmation of membership of nominee: ( Active ( Emeritus (
>Non-member
>Year nominee joined ABSA
>ABSA Activities (offices, committees, etc)
>
>
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
=========================================================================
Date: Wed, 7 Jul 1999 08:30:26 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jairo Betancourt
Subject: Re: BSL-3 exhaust system
MIME-version: 1.0
Content-type: text/plain; charset="iso-8859-1"
Content-transfer-encoding: 7bit
Jose: We had a project last year with M. tuberculosis and monkeys in our
BSL3 facility and went through all kinds of adjustments and objections. It
would be too long for the list to describe what we encounter and what we had
to do and because each BSL3 facility could different in design and location.
You can call me and discuss it if you wish. However I can summarize it in a
few words:
1. The exhaust from the BSL3 facility on the roof was at eye level
2. Most utility controls and mechanical rooms are on the roof, where
Physical Plant employees and contractors will go on a daily basis sometimes
for extended periods of time (preventive maintenance and repairs).
3. The exhaust did not have HEPA filters.
Solutions?
1. HEPA filter the air coming from the BSL3 facility
2. Extend the exhaust stacks high enough ( a la ASHRAE guidelines) therefore
affecting the balance of ventilation
3. Fit test all the involve Physical Plant employees and give them the
respirators after the proper training?
4. restrict completely the access to the roof
5. all of the above
For reasons beyond my control (political) it was decided that the solution
was # 3. This caused a lot of problems because the Physical Plant employees
got really upset, paranoid, etc and some refused to work on the roof. After
meetings and meetings I finally convinced the Administration (using the BMBL
4th coming edition) to retrofit the whole ventilation and install HEPA
filters in the exhaust. Weare now in the process of doing that. Although I
will always prefer to HEPA filter the exhaust of any BSL3 facility, you do
not have to do that. Think about ASHRAE guidelines for the the fume hood
exhausts stacks, having in consideration the highest point of the building,
the area average wind velocity, etc and the high of adjacent building. At
the end you will have to balance the Lab ventilation anyway to make negative
and directional.
Sorry for being so general, but it would take too long for the list.
Thank you
Jairo Betancourt
=========================================================================
Date: Wed, 7 Jul 1999 08:43:29 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Kyle Boyett
Subject: Re: BSL-3 exhaust system
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Jose, There are a lot variables associated with exhaust ducting of biosafety
cabinets. One such variable would involve the prevailing winds in your part
of the country and the height of adjacent buildings. But without getting
into all that, as a general rule if you have the ability and financial
resources it is best to duct the exhaust on the opposite side of the
building. We employ the standard 7 foot no loss stack heights for all our
fume hood and biosafety cabinet exhaust. This 7 foot measurement is in
respect to the highest point of the building. For example, if the roof has
multiple levels the stack would be a minimum of 7 feet above the highest
point. Using this 7 foot design which would also include a high terminal
velocity, the potential contaminated plume would be high above anyone's
breathing zone and hopefully above any air intake for the building. Now
that should address the entrainment aspect of your question. HEPA filtering?
Yes. Depending on where the lab is located in the building and the
difficulty of decontaminating several floors of ductwork, you will probably
find it more practical to locate the filter box in the interstitial space
above the lab. We had a recent problem with a BSL 3 area where we did just
that and so far everything seems to be working fine. You can call me if you
would like more information. Hopes this helps you a bit.
Kyle Boyett
Asst. Director of Biosafety
Occupational Health and Safety
University of Alabama at Birmingham
e-mail- kboyett@healthsafe.uab.edu
Phone- 205-934-2487
** Asking me to overlook a safety violation is like asking me to reduce the
value I place on YOUR life**
-----Original Message-----
From: Jose Vazquez Perez [mailto:jvazquez@MAIL.UCF.EDU]
Sent: Tuesday, July 06, 1999 7:40 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: BSL-3 exhaust system
Dear Biosafety Folks,
I am involved in the process of designing the upgrade of a lab from
level 2 to level 3 for use with Mycobacterium tuberculosis, and would
appreciate any ideas or suggestions in interpreting the following
passage from the BMBL (4th edition) on the issue of an exhaust air
ventilation system:
"The outside exhaust must be dispersed away from occupied areas and air
intakes, or the exhaust must be HEPA-filtered." (item 9, p. 34.)
Specifically, I am interested in suggestions as to what mechanisms we
can use to 'disperse exhaust air away from outside air intakes' of air
handling units, e.g., do we require a certain distance between them (if
so, how far? 25, 50, 100 feet), or do we require the contractor to
exhaust the air in the direction opposite to any air intakes?
Thank you again,
Jose Vazquez
Biosafety Coordinator
Environmental Health & Safety
(407) 823-6301
jvazquez@mail.ucf.edu
=========================================================================
Date: Wed, 7 Jul 1999 08:58:46 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: The new CDC/NIH BMBL (4th Ed.)
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Dear Pamela:
The ad with my copy and the nice little book mark inside the copy CDC
sent me indicate the web site has the fourth edition and I trusted it was
up and running. But, alas it is summer and also hot is Atlanta and
Washington and the Biosafty chat group messages now number some ten
telling me that the bookmark and AD are incorrect. It ain't there yet!
Sorry! I am sure as soon as the temperature in Atlanta get back to 95 F
someone will push the right button and the 4th Ed will be available to all.
Again, it is much upgraded and improved and the preface nicely summarizes
most of the changes and additions for those reading novels instead of
Biosafety Manuals this wonderful summer.
Diane Fleming, the beautiful and most knowledgeable biosafety Expert I
know, tells me that the price has risen to $12.00 per copy and that seems
> than a 100% increase, but may be volume discounts are available. Diane
also noted the Phone number listed is incorrect and in now (202) 512-1800.
As I noted earlier, the editors, section writers, and ABSA Techical Rev
Commitee led by dear friend, the also beautiful Rich Rebar, did a swell job
and deserve all our thanks.
Anyway, sit back, relax, have some lemonade or a good alcoholic beverage
of your choice and I'll loan you my copy as soon as I finish the last
section. Let me know if you need it stat.
Regards,
Joe Coggin, Jr.
On Tue, 6 Jul 1999, Pamela Hubley wrote:
> Hi Joseph,
>
> I find the 3rd edition posted on the site you listed below. Where should I
> be looking?
>
> Pamela Hubley, CIH, CSP
> Millipore Corp.
> Email: pamela_hubley@
>
>
>
>
>
> "Joseph H. Coggin Jr." on 07/06/99 12:26:54
> PM
>
> Please respond to A Biosafety Discussion List
>
> To: BIOSAFTY@MITVMA.MIT.EDU
> cc: (bcc: Pamela Hubley/NA/Millipore)
>
> Subject: Re: The new CDC/NIH BMBL (4th Ed.)
>
>
>
>
> Julie:
>
> On Tue, 6 Jul 1999, Johnson, Julie A. wrote:
>
> > What is the web site address where you can find the 4th edition? I have
> > only been able to find the 3rd edition on-line.
> >
> > Julie A. Johnson, Ph.D.
> > Biosafety Officer
> > Environmental Health and Safety
> > Iowa State University
> > Ames, IA 50011
> > e-mail: jajohns@iastate.edu
> > phone: 515-294-7657
> > fax: 515-294-9357
> > web site:
> >
> >
> > -----Original Message-----
> > From: Joseph H. Coggin Jr. [mailto:jcoggin@JAGUAR1.USOUTHAL.EDU]
> > Sent: Tuesday, July 06, 1999 11:14 AM
> > To: BIOSAFTY@MITVMA.MIT.EDU
> > Subject: Re: The new CDC/NIH BMBL (4th Ed.)
> >
> >
> > Robin:
> > I suggest we all read it and digest the new edition as it is very helpful
> > and represents much hard work by the guest editors and Jonathan
> > Richmond and Bob McKinney. Our thanks goes out most sincerely to all who
> > helped get this edition out. We needed it badly!
> >
> > Get it from the Web site or order it from US GOV. Printing Office.
> >
> > FYI: The major changes are summarized on page viii and ix by the editors.
> >
> > Joe Coggin, Jr. Ph.D.
> > RBP, CBSP
> > Professor and Chairman,
> > U. South Alabama, Coll. of Medicine
> > LMB,
> > Mobile, AL 36688
> >
> > On Tue, 6 Jul 1999, Robin Newberry wrote:
> >
> > > I just got a copy of the 4th Ed. of the BMBL; I've not had time to
> > > read it yet, but I've a couple of questions:
> > >
> > > 1) Are there substantial changes from the 3rd Ed.?
> > >
> > > 2) You used to be able to get free copies, now there is only info on
> > > how to buy it from the GPO. Are free copies still available to
> > > Universities and State Agencies?
> > >
> > > Many TIA,
> > >
> > > Robin
> > >
> > > W. Robert Newberry, IV CIH, CHMM
> > > Director, Environmental Health and Safety
> > > Clemson University
> > >
> > > wnewber@clemson.edu ehs@clemson.edu
> > >
> > >
> > > ################## NOTICE TO BULK E-MAILERS ###################
> > > ##### PLEASE READ THIS BEFORE BULK E-MAILING THIS ADDRESS #####
> > > Pursuant to US Code, Title 47, Chapter 5, Subchapter II, p.227, any
> > > and all non-solicited commercial E-mail sent to the address of the
> > > sender of this message is subject to a download and archival fee in
> > > the amount of $500.00 US. Anyone who sends unsolicited commercial
> > > e-mail to this account will be charged a $500.00 US proofreading fee.
> > > This is an official notification. Failure to abide by this may result
> > > in civil and/or criminal legal action as allowed under United States
> > > law. For a complete summary of this legislation see:
> > >
> > > ###############################################################
> > >
> >
>
=========================================================================
Date: Wed, 7 Jul 1999 10:05:33 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: fixed cells
MIME-Version: 1.0
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Bliss,
Tissue is usually fixed in some type of aldehyde (glutaraldehyde,
formaldehyde, whatever). This preserves the cellular structure. It is then
generally processed through a series of dehydration steps (alcohol, xylene
or propylene oxide) and embedded in paraffin or epoxy for sectioning and
microscopy. There are other processes used, however, (frozen sections,
etc.) and you should check with a histologist to find out precisely what
they are doing if you have questions.
Fixed tissue is generally considered free of pathogens or any form of life.
There is some evidence that prions can remain infective, however. Probably
the web sites from the UK would be a good source of info since they have
more experience with CJD & BSE. Unless you have reason to suspect prions,
fixed tissue would generally be considered safe from infectivity.
As someone mentioned, histology and electron microscopy labs are full of
hazardous chemicals. That's another issue. Good Luck.
Janice Flesher, MS
Principle Industrial Hygienist/Biosafety Officer
EOHSS - University Medical Dental School of NJ
97 Paterson St. #227
New Brunswick, NJ, 08901
(732) 235-8497 phone
(732) 235-8499 fax
fleshejk@umdnj.edu
-----Original Message-----
From: Schlank Bliss BM
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Tuesday, July 06, 1999 1:24 PM
Subject: fixed cells
> was wondering if anyone had a reference to "fixed" tissues, human cells,
>viruses, etc. and the safety it offers to the user.
>
>Is the agent that is fixed rendered non-infectious through this process?
>
>Do you always use paraformaldehyde?
>
>I would also like a actual procedure for this "fixing" process.
>
>Thank you and I hope everyone enjoyed the fourth!
>
>Bliss M. Schlank
>Biosafety Specialist
>AstraZeneca
>1800 Concord Pike
>Wilmington DE 19850-5437
>302.886.2185 Fax: 302.886.2909
>bliss.schlank@phwilm.
>
>
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=========================================================================
Date: Wed, 7 Jul 1999 10:27:21 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Norman, Randy"
Subject: Re: fixed cells
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
For info on handling tissues from patients with suspected prion diseases,
see pp140 and 143 of the new 4th edition of BMBL. Most folks are using a
formic acid step (BMBL 4th ed. says 95% absolute formic acid for 30 minutes)
followed by a good (e.g., 48 hrs plus) soak in fresh 10% formalin, prior to
the usual embedding procedures.
Randy Norman
Safety Specialist Sr.
BioReliance Corporation
Rockville, MD 20850
Rnorman@
"Success is a journey, not a destination" - Ben Sweetland
=========================================================================
Date: Wed, 7 Jul 1999 10:33:58 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Norman, Randy"
Subject: Re: Fixed Cells
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
For info on handling tissues from patients with suspected prion diseases,
see pp140 and 143 of the new 4th edition of BMBL. Most folks are using a
formic acid step (BMBL 4th ed. says 95% absolute formic acid for 30 minutes)
followed by a good (e.g., 48 hrs plus) soak in fresh 10% formalin, prior to
the usual embedding procedures.
Randy Norman
Safety Specialist Sr.
BioReliance Corporation
Rockville, MD 20850
Rnorman@
"Success is a journey, not a destination" - Ben Sweetland
=========================================================================
Date: Wed, 7 Jul 1999 09:57:23 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Al Jin
Subject: Re: BSL-3 exhaust system
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Jose,
I would like to add to Jairo, and Kyle's comments regarding stack height
and location. I would like to bring your attention to figure 8-7 of the
ACGIH Industrial Ventilation Book (latested edition). The general rule for
a preferred height for a building exhaust system is approximately 1.3 - 2.0
times the height of the building. (Sorry, this rule may not apply to
skyscrappers).
Be careful of down drafts and direction of prevealing winds. To determine
the distance away from the nearest intake duct, one must use the Gaussian
Dispersion Model or the D.J. Wilson Model that takes into account of stack
height, worst-case wind conditions in all directions. In the old days, one
would have to use brute force to wrestle with the formulas based on the
applicable chemcials being used in the laboratory. Now days, one can use a
computer model to determine the exact distance between intake and exhaust
ducts.
In closing, use the 1.3 - 2.0x Rule and put intakes upwind of the exhaust
ducts. Also see section 5.16: Exhaust Stack Outlets of the ACGIH
Ventilation Manual for 13 key ventilation considerations for stack
designs. Best of Luck.
Alfred Jin, BSO, IH, CBSP, M(ASCP), BSS(ASM), CM(ACM),
Hazards Control Department,
Lawrence Livermore National Laboratory,
7000 East Avenue MS-289,
Livermore, CA 94550,
Phone:925 423-7385, Fax:423-1086,
Jin2@
>Jose, There are a lot variables associated with exhaust ducting of biosafety
>cabinets. One such variable would involve the prevailing winds in your part
>of the country and the height of adjacent buildings. But without getting
>into all that, as a general rule if you have the ability and financial
>resources it is best to duct the exhaust on the opposite side of the
>building. We employ the standard 7 foot no loss stack heights for all our
>fume hood and biosafety cabinet exhaust. This 7 foot measurement is in
>respect to the highest point of the building. For example, if the roof has
>multiple levels the stack would be a minimum of 7 feet above the highest
>point. Using this 7 foot design which would also include a high terminal
>velocity, the potential contaminated plume would be high above anyone's
>breathing zone and hopefully above any air intake for the building. Now
>that should address the entrainment aspect of your question. HEPA filtering?
>Yes. Depending on where the lab is located in the building and the
>difficulty of decontaminating several floors of ductwork, you will probably
>find it more practical to locate the filter box in the interstitial space
>above the lab. We had a recent problem with a BSL 3 area where we did just
>that and so far everything seems to be working fine. You can call me if you
>would like more information. Hopes this helps you a bit.
>
>Kyle Boyett
>Asst. Director of Biosafety
>Occupational Health and Safety
>University of Alabama at Birmingham
>e-mail- kboyett@healthsafe.uab.edu
>Phone- 205-934-2487
>
>** Asking me to overlook a safety violation is like asking me to reduce the
>value I place on YOUR life**
>
>-----Original Message-----
>From: Jose Vazquez Perez [mailto:jvazquez@MAIL.UCF.EDU]
>Sent: Tuesday, July 06, 1999 7:40 PM
>To: BIOSAFTY@MITVMA.MIT.EDU
>Subject: BSL-3 exhaust system
>
>
>Dear Biosafety Folks,
>I am involved in the process of designing the upgrade of a lab from
>level 2 to level 3 for use with Mycobacterium tuberculosis, and would
>appreciate any ideas or suggestions in interpreting the following
>passage from the BMBL (4th edition) on the issue of an exhaust air
>ventilation system:
>"The outside exhaust must be dispersed away from occupied areas and air
>intakes, or the exhaust must be HEPA-filtered." (item 9, p. 34.)
>Specifically, I am interested in suggestions as to what mechanisms we
>can use to 'disperse exhaust air away from outside air intakes' of air
>handling units, e.g., do we require a certain distance between them (if
>so, how far? 25, 50, 100 feet), or do we require the contractor to
>exhaust the air in the direction opposite to any air intakes?
>Thank you again,
>
>
>Jose Vazquez
>Biosafety Coordinator
>Environmental Health & Safety
>(407) 823-6301
>jvazquez@mail.ucf.edu
=========================================================================
Date: Wed, 7 Jul 1999 11:00:11 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Al Jin
Subject: Re: BSL-3 exhaust system
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Jose,
I would like to add to Jairo, and Kyle's comments regarding stack height
and location. I would like to bring your attention to figure 8-7 of the
ACGIH Industrial Ventilation Book (latested edition). The general rule for
a preferred height for a building exhaust system is approximately 1.3 - 2.0
times the height of the building. (Sorry, this rule may not apply to
skyscrappers).
Be careful of down drafts and direction of prevealing winds. To determine
the distance away from the nearest intake duct, one must use the Gaussian
Dispersion Model or the D.J. Wilson Model that takes into account of stack
height, worst-case wind conditions in all directions. In the old days, one
would have to use brute force to wrestle with the formulas based on the
applicable chemcials being used in the laboratory. Now days, one can use a
computer model to determine the exact distance between intake and exhaust
ducts.
In closing, use the 1.3 - 2.0x Rule and put intakes upwind of the exhaust
ducts. Also see section 5.16: Exhaust Stack Outlets of the ACGIH
Ventilation Manual for 13 key ventilation considerations for stack
designs. Best of Luck.
Alfred Jin, BSO, IH, CBSP, M(ASCP), BSS(ASM), CM(ACM),
Hazards Control Department,
Lawrence Livermore National Laboratory,
7000 East Avenue MS-289,
Livermore, CA 94550,
Phone:925 423-7385, Fax:423-1086,
Jin2@
>Dear Biosafety Folks,
>I am involved in the process of designing the upgrade of a lab from
>level 2 to level 3 for use with Mycobacterium tuberculosis, and would
>appreciate any ideas or suggestions in interpreting the following
>passage from the BMBL (4th edition) on the issue of an exhaust air
>ventilation system:
>"The outside exhaust must be dispersed away from occupied areas and air
>intakes, or the exhaust must be HEPA-filtered." (item 9, p. 34.)
>Specifically, I am interested in suggestions as to what mechanisms we
>can use to 'disperse exhaust air away from outside air intakes' of air
>handling units, e.g., do we require a certain distance between them (if
>so, how far? 25, 50, 100 feet), or do we require the contractor to
>exhaust the air in the direction opposite to any air intakes?
>Thank you again,
>
>
>Jose Vazquez
>Biosafety Coordinator
>Environmental Health & Safety
>(407) 823-6301
>jvazquez@mail.ucf.edu
=========================================================================
Date: Wed, 7 Jul 1999 14:52:36 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Taylor, David G. PHD"
Subject: Re: The new CDC/NIH BMBL (4th Ed.)
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
All,
We appreciate Joe Coggin's discussion of the 4th Edition of the BMBL. It is
hot in Atlanta as well as much of the East Coast. We have been trying to
improve the presentation of the new figures in this version as well as
converting our website to a 'new look'. We hope you like it.
The 4th Edition will be posted by July 16. You have also noted an erratum.
The correction is as follows.
The correct GPO telephone ordering information is: Call the GPO Order Desk
between 7:30 a.m. and 4:30 p.m. eastern time, Monday through Friday, at
1-202-512-1800 or fax at 1-202-512-2250.
Or you can order on-line at URL
biological+and+Biomedical+Laboratories&stock=017-040-00547-4&price=12.00&lis
tid=&orderFN=&resultPageFN=/diskb/cgi-bin-tmp/RSLTaaaaabxla
David G. Taylor, Ph.D., CBSP
Deputy Director
CDC Office of Health and Safety
DGT1@
-----Original Message-----
From: Joseph H. Coggin Jr. [mailto:jcoggin@JAGUAR1.USOUTHAL.EDU]
Sent: Wednesday, July 07, 1999 9:59 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: The new CDC/NIH BMBL (4th Ed.)
Dear Pamela:
The ad with my copy and the nice little book mark inside the copy CDC
sent me indicate the web site has the fourth edition and I trusted it was
up and running. But, alas it is summer and also hot is Atlanta and
Washington and the Biosafty chat group messages now number some ten
telling me that the bookmark and AD are incorrect. It ain't there yet!
Sorry! I am sure as soon as the temperature in Atlanta get back to 95 F
someone will push the right button and the 4th Ed will be available to all.
Again, it is much upgraded and improved and the preface nicely summarizes
most of the changes and additions for those reading novels instead of
Biosafety Manuals this wonderful summer.
Diane Fleming, the beautiful and most knowledgeable biosafety Expert I
know, tells me that the price has risen to $12.00 per copy and that seems
> than a 100% increase, but may be volume discounts are available. Diane
also noted the Phone number listed is incorrect and in now (202) 512-1800.
As I noted earlier, the editors, section writers, and ABSA Techical Rev
Commitee led by dear friend, the also beautiful Rich Rebar, did a swell job
and deserve all our thanks.
Anyway, sit back, relax, have some lemonade or a good alcoholic beverage
of your choice and I'll loan you my copy as soon as I finish the last
section. Let me know if you need it stat.
Regards,
Joe Coggin, Jr.
On Tue, 6 Jul 1999, Pamela Hubley wrote:
> Hi Joseph,
>
> I find the 3rd edition posted on the site you listed below. Where should
I
> be looking?
>
> Pamela Hubley, CIH, CSP
> Millipore Corp.
> Email: pamela_hubley@
>
>
>
>
>
> "Joseph H. Coggin Jr." on 07/06/99 12:26:54
> PM
>
> Please respond to A Biosafety Discussion List
>
> To: BIOSAFTY@MITVMA.MIT.EDU
> cc: (bcc: Pamela Hubley/NA/Millipore)
>
> Subject: Re: The new CDC/NIH BMBL (4th Ed.)
>
>
>
>
> Julie:
>
> On Tue, 6 Jul 1999, Johnson, Julie A. wrote:
>
> > What is the web site address where you can find the 4th edition? I have
> > only been able to find the 3rd edition on-line.
> >
> > Julie A. Johnson, Ph.D.
> > Biosafety Officer
> > Environmental Health and Safety
> > Iowa State University
> > Ames, IA 50011
> > e-mail: jajohns@iastate.edu
> > phone: 515-294-7657
> > fax: 515-294-9357
> > web site:
> >
> >
> > -----Original Message-----
> > From: Joseph H. Coggin Jr. [mailto:jcoggin@JAGUAR1.USOUTHAL.EDU]
> > Sent: Tuesday, July 06, 1999 11:14 AM
> > To: BIOSAFTY@MITVMA.MIT.EDU
> > Subject: Re: The new CDC/NIH BMBL (4th Ed.)
> >
> >
> > Robin:
> > I suggest we all read it and digest the new edition as it is very
helpful
> > and represents much hard work by the guest editors and Jonathan
> > Richmond and Bob McKinney. Our thanks goes out most sincerely to all
who
> > helped get this edition out. We needed it badly!
> >
> > Get it from the Web site or order it from US GOV. Printing Office.
> >
> > FYI: The major changes are summarized on page viii and ix by the
editors.
> >
> > Joe Coggin, Jr. Ph.D.
> > RBP, CBSP
> > Professor and Chairman,
> > U. South Alabama, Coll. of Medicine
> > LMB,
> > Mobile, AL 36688
> >
> > On Tue, 6 Jul 1999, Robin Newberry wrote:
> >
> > > I just got a copy of the 4th Ed. of the BMBL; I've not had time to
> > > read it yet, but I've a couple of questions:
> > >
> > > 1) Are there substantial changes from the 3rd Ed.?
> > >
> > > 2) You used to be able to get free copies, now there is only info on
> > > how to buy it from the GPO. Are free copies still available to
> > > Universities and State Agencies?
> > >
> > > Many TIA,
> > >
> > > Robin
> > >
> > > W. Robert Newberry, IV CIH, CHMM
> > > Director, Environmental Health and Safety
> > > Clemson University
> > >
> > > wnewber@clemson.edu ehs@clemson.edu
> > >
> > >
> > > ################## NOTICE TO BULK E-MAILERS ###################
> > > ##### PLEASE READ THIS BEFORE BULK E-MAILING THIS ADDRESS #####
> > > Pursuant to US Code, Title 47, Chapter 5, Subchapter II, p.227, any
> > > and all non-solicited commercial E-mail sent to the address of the
> > > sender of this message is subject to a download and archival fee in
> > > the amount of $500.00 US. Anyone who sends unsolicited commercial
> > > e-mail to this account will be charged a $500.00 US proofreading fee.
> > > This is an official notification. Failure to abide by this may result
> > > in civil and/or criminal legal action as allowed under United States
> > > law. For a complete summary of this legislation see:
> > >
> > > ###############################################################
> > >
> >
>
=========================================================================
Date: Wed, 7 Jul 1999 15:13:20 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Taylor, David G. PHD"
Subject: Re: BSL-3 exhaust system
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Another solution is to use an exhaust fan called a Tri-Stack by Strobic Air
Corp. that exhausts the air above the air flowing over the building. See
.
Dave Taylor
CDC
-----Original Message-----
From: Jairo Betancourt [mailto:jairob@MIAMI.EDU]
Sent: Wednesday, July 07, 1999 8:30 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: BSL-3 exhaust system
Jose: We had a project last year with M. tuberculosis and monkeys in our
BSL3 facility and went through all kinds of adjustments and objections. It
would be too long for the list to describe what we encounter and what we had
to do and because each BSL3 facility could different in design and location.
You can call me and discuss it if you wish. However I can summarize it in a
few words:
1. The exhaust from the BSL3 facility on the roof was at eye level
2. Most utility controls and mechanical rooms are on the roof, where
Physical Plant employees and contractors will go on a daily basis sometimes
for extended periods of time (preventive maintenance and repairs).
3. The exhaust did not have HEPA filters.
Solutions?
1. HEPA filter the air coming from the BSL3 facility
2. Extend the exhaust stacks high enough ( a la ASHRAE guidelines) therefore
affecting the balance of ventilation
3. Fit test all the involve Physical Plant employees and give them the
respirators after the proper training?
4. restrict completely the access to the roof
5. all of the above
For reasons beyond my control (political) it was decided that the solution
was # 3. This caused a lot of problems because the Physical Plant employees
got really upset, paranoid, etc and some refused to work on the roof. After
meetings and meetings I finally convinced the Administration (using the BMBL
4th coming edition) to retrofit the whole ventilation and install HEPA
filters in the exhaust. Weare now in the process of doing that. Although I
will always prefer to HEPA filter the exhaust of any BSL3 facility, you do
not have to do that. Think about ASHRAE guidelines for the the fume hood
exhausts stacks, having in consideration the highest point of the building,
the area average wind velocity, etc and the high of adjacent building. At
the end you will have to balance the Lab ventilation anyway to make negative
and directional.
Sorry for being so general, but it would take too long for the list.
Thank you
Jairo Betancourt
=========================================================================
Date: Wed, 7 Jul 1999 15:32:17 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Don Callihan
Subject: modular BSL3 labs
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I recall seeing an ad for modular BSL3 labs recently but can't find the source.
Would appreciate it if anyone could identify the company (and their phone
number). More importantly, has anyone had direct experience with this or a
similar product?
Thank you for your collective wisdom.
Don Callihan
Biosafety Officer
BD Biosciences
P.O. Box 999 - MC982
Sparks, MD 21152-0999
Phone: 410.773.6684
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=========================================================================
Date: Thu, 8 Jul 1999 08:04:24 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Rick Gulizia
Subject: Re: modular BSL3 labs
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
> Don,
There is a company in Canada that manufactures a BSL-3 modular set up. The
Company is Microzone and they have a web site with all
the contact numbers. We have one here on campus and I have nothing but
good things to say about the product. The microzone people are
knowledgeable and are flexible with the design of the BSL-3 labs. Hope
this helps.
>
>I recall seeing an ad for modular BSL3 labs recently but can't find the source.
>Would appreciate it if anyone could identify the company (and their phone
>number). More importantly, has anyone had direct experience with this or a
>similar product?
>
>Thank you for your collective wisdom.
>
>Don Callihan
>Biosafety Officer
>BD Biosciences
>P.O. Box 999 - MC982
>Sparks, MD 21152-0999
>Phone: 410.773.6684
Rick Gulizia
Director P3 Facilities
The Scripps Research Institute
10550 No. Torrey Pines Road, TPC-23
La Jolla , CA
Office: 619-784-9216
Fax: 619-784-8722
Pager: 619-494-8451
=========================================================================
Date: Thu, 8 Jul 1999 16:55:09 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Larry Thompson
Subject: disinfectant . . .
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Group,
I have been asked about a relatively new disinfectant named "Virkon S". (I
say relatively new because I hadn't heard of it...). I have contacted the
company for information (its in the mail...).
What is it? Uses? Hazards?
Thanks,
Larry
Larry J. Thompson, DVM PhD
Director of Biosafety
College of Veterinary Medicine
Cornell University
Upper Tower Road phone 607-253-3900
Ithaca, NY 14853-6401 fax 607-253-3943
"There is no limit to the good you can do if you don't care who gets the
credit."
Gen. George Marshall
=========================================================================
Date: Thu, 8 Jul 1999 16:07:53 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: Re: disinfectant . . .
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I looked into using Virkon for a specific use a few months ago, but ended up
choosing to go with a less expensive and effective alternative. The company
that makes Virkon, Antec International, has a lot of information about it on
their web site, including efficacy testing. They claim EPA approval, but I
could not find it on the EPA registered lists at the time.
The web site address is:
Julie A. Johnson, Ph.D.
Biosafety Officer
Environmental Health and Safety
Iowa State University
Ames, IA 50011
e-mail: jajohns@iastate.edu
phone: 515-294-7657
fax: 515-294-9357
web site:
-----Original Message-----
From: Larry Thompson [mailto:ljt2@CORNELL.EDU]
Sent: Thursday, July 08, 1999 3:55 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: disinfectant . . .
Group,
I have been asked about a relatively new disinfectant named "Virkon S". (I
say relatively new because I hadn't heard of it...). I have contacted the
company for information (its in the mail...).
What is it? Uses? Hazards?
Thanks,
Larry
Larry J. Thompson, DVM PhD
Director of Biosafety
College of Veterinary Medicine
Cornell University
Upper Tower Road phone 607-253-3900
Ithaca, NY 14853-6401 fax 607-253-3943
"There is no limit to the good you can do if you don't care who gets the
credit."
Gen. George Marshall
=========================================================================
Date: Fri, 9 Jul 1999 08:49:32 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: disinfectant . . .
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
For info about Virkon S go to:
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Fri, 9 Jul 1999 10:52:41 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Dave Gelpke
Subject: Clean Laboratory Glass
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Any suggestions what to do with clean broken lab glass?
Are there services to pick up this glass?
Thanks
=========================================================================
Date: Fri, 9 Jul 1999 12:35:57 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Francis Churchill
Subject: BMBL (4th Ed.) biohaz sign
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
One change that I've noticed at the first skim of BMBL (4th ed) is that
Standard Micro Practices now include 10 recommendations. Number 9 says, "A
biohazard sign must be posted at the entrance to the laboratory whenever
infectious agents are present." This is part of the "standard and special
practices, safety equipment and facilities [that] apply to agents assigned
to Biosafetly Level 1" as well as to other biosafety levels.
My confusion is whether a BSL 1 organism is an "infectious agent." Is a
biohazard sign required (not just a good idea) on a BSL1 lab?
Thanks for your thoughts,
Francis
=========================================================================
Date: Fri, 9 Jul 1999 12:46:33 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Francis Churchill
Subject: Re: Clean Laboratory Glass
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
>Any suggestions what to do with clean broken lab glass?
>Are there services to pick up this glass?
If it is free of hazardous residues we send it out in the recycling, unless
it is pyrex or borosilicate in which case it goes in our solid waste/trash.
The important point is that it goes into a "broken glass" container
(cardborad box) and not into the regular trash where a custodian can be
injred by it.
Francis
Alcohol and calculus don't mix. Never drink and derive.
Francis Churchill, IHIT
University of Vermont - Environmental Safety Facility
655D Spear Street, Burlington, VT 05405-3010
(802) 656-5405
fchurchi@zoo.uvm.edu
=========================================================================
Date: Fri, 9 Jul 1999 13:10:19 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Clean Laboratory Glass
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Our policy is fairly straight forward.
Contaminated glassware goes into proper biohazard sharps containers. Even
intact glass bottles. This is removed as biohazardous waste.
Non-contaminated glass will be disposed of in any box, it will weigh no
more than 60 pounds, will be sealed and have written on the outside,
"Broken Glass, Sharps". The box must hold the glass with no breaches. It
can be removed with the regular trash.
Bottles are placed in one of the two above receptacles. Caps are removed
and the labels are removed or defaced. The bottle is marked "empty" or
"mt".
Bottles will break and then become sharps. We treat them as sharps from
the beginning.
bob
>Any suggestions what to do with clean broken lab glass?
>Are there services to pick up this glass?
>Thanks
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Fri, 9 Jul 1999 12:16:06 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Christina Z Thompson
Subject: Re: BMBL (4th Ed.) biohaz sign
MIME-version: 1.0
Content-type: text/plain; charset=us-ascii
It appears to me that the "Standard Microbiological Practices" have been
reversed for BL-1 and BL-2, as the practices listed under BL-2 do not mention
a biohazard sign (there are only 9 practices listed for BL-2). I believe that
posting BL-1 labs with the biohazard sign is wrong, as the definition of BL-1
is "agents that are not known to consistently cause disease in healthy adult
humans...". If not infectious (under normal circumstances), and no special
practices or precautions are necessary, why post a sign? We then run into
what our "house vet" likes to call sign pollution.
Chris Thompson
Biosafety Officer
Eli Lilly & Company
=========================================================================
Date: Fri, 9 Jul 1999 13:10:42 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Norman, Randy"
Subject: Re: BMBL (4th Ed.) biohaz sign
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
A VERY interesting point. If this requirement is not a typo, then it
represents additional focus on BSL 1 which could certainly lead to some
interesting discussion. My "take" on the definition of Biosafety Level 1 and
"infectious agent" is that Biosafety Level 1 is for, among other things, any
agent that we know to be an opportunistic pathogen. Though they only infect
the immunocompromised, I would still call them "infectious agents" myself.
I realize I probably mention this too many times, but as a Biosafety pro on
chemotherapy, I have a keen personal interest in the hazards faced by
immunocompromised populations which certainly affects my view on this and
similar issues.
Should a sign be required? I suppose it depends in part upon whether
immunocompromised persons work in the building and may otherwise walk in,
unaware of the potential hazard to their health.
Yes a very interesting idea. And in my opinion yes a sign should be
required.
Randy Norman
Safety Specialist Sr.
BioReliance Corporation
Rockville, MD 20850
Rnorman@
"Success is a journey, not a destination" - Ben Sweetland
=========================================================================
Date: Fri, 9 Jul 1999 13:15:13 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Norman, Randy"
Subject: Re: BMBL (4th Ed.) biohaz sign
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
>It appears to me that the "Standard Microbiological Practices" have
been
>reversed for BL-1 and BL-2, as the practices listed under BL-2 do
not mention
>a biohazard sign (there are only 9 practices listed for BL-2).
I disagree, as the requirements for signs for BSL 2 labs are found in
section B.3. on page 21 of the 4th edition. And for the reasons I just
stated.
Randy Norman
Safety Specialist Sr.
BioReliance Corporation
Rockville, MD 20850
Rnorman@
"Success is a journey, not a destination" - Ben Sweetland
=========================================================================
Date: Fri, 9 Jul 1999 13:33:33 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Mike Pascucilla
Subject: food service establishment licensing
Hello Biosafety Community:
Part of our universities biological health and safety program focuses
on foodborne illness prevention and we solely inspect all the food
service establishments on campus, without local or state health
dept. assistance. We are currently developing a licensing program
and a sanitary food code. Does anyone have any information or a
similar program on their campus? Any information will be greatly
appreciated. Please email me directly.
Thank you,
Michael Pascucilla, R.S.
Environmental Health and Safety
3102 Horsebarn Hill Rd, Box U-97
Storrs, CT 06269
Phone: (860)486-3613
Fax: (860) 486-1160
E-Mail: Mike@safety.ehs.uconn.edu
=========================================================================
Date: Fri, 9 Jul 1999 14:51:20 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: food service establishment licensing
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Mike,
Princeton University does a similar thing. The sanitarian and biosafety
officer is Donald Robasser at robosser@princeton.edu.
As a former public health official, my advice to you is not to reinvent the
wheel. Adopt the FDA Model Food Code or at the very least, the Food Code
for Conn. As you may know, information on food safety and emerging
pathogens is changing rapidly. Mistakes are costly both in dollars and
lives. The National Restaurant Association has excellent training materials
and a manager's certification program. Good luck!
Janice Flesher, MS
Principle Industrial Hygienist/Biosafety Officer
EOHSS - University Medical Dental School of NJ
97 Paterson St. #227
New Brunswick, NJ, 08901
(732) 235-8497 phone
(732) 235-8499 fax
fleshejk@umdnj.edu
-----Original Message-----
From: Mike Pascucilla
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Friday, July 09, 1999 2:06 PM
Subject: food service establishment licensing
>Hello Biosafety Community:
>
>Part of our universities biological health and safety program focuses
>on foodborne illness prevention and we solely inspect all the food
>service establishments on campus, without local or state health
>dept. assistance. We are currently developing a licensing program
>and a sanitary food code. Does anyone have any information or a
>similar program on their campus? Any information will be greatly
>appreciated. Please email me directly.
>
>Thank you,
>Michael Pascucilla, R.S.
>Environmental Health and Safety
>3102 Horsebarn Hill Rd, Box U-97
>Storrs, CT 06269
>Phone: (860)486-3613
>Fax: (860) 486-1160
>E-Mail: Mike@safety.ehs.uconn.edu
>
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ORG:University Medical Dental of NJ;EOHSS
TITLE:Principle industrial Hygienist/Biosafety Officer
TEL;WORK;VOICE:732-235-8497
TEL;HOME;VOICE:609-620-1206
TEL;WORK;FAX:732-235-8499
ADR;WORK:;;97 Paterson Street Ste 227;New Brunswick;NJ;08903;USA
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=========================================================================
Date: Fri, 9 Jul 1999 17:11:54 EDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Diane Fleming
Subject: Re: BMBL (4th Ed.) biohaz sign
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Just because the agent doesn't consistently (new word added in this
edition) cause disease in healthy adult humans does not mean it is not
infectious. Thus, even at BSL 1, a warning sign is appropriate. Canine
hepatitis virus is an animal pathogen which does not include healthy adult
humans in its host range, but it is infectious. Other BSL 1 agents can cause
disease in the immunosuppressed or immunocompromised, the elderly and the
very young, or in plants as well as animals. It seems appropriate to warn of
the hazard to these groups.
Regarding Chris's question: BSL 2 uses all of the practices of BSL !
and the ones additionally listed for level 2 as in Table 1 (pg52 of BMBL).
Hope that helps. Diane
Diane O. Fleming, Ph.D., CBSP
Biosafety Consultant
15611 Plumwood Court
Bowie, MD 20716-1434
Tel 301-249-3951
FAX 301-249-8837
email Dimerck@
=========================================================================
Date: Sun, 11 Jul 1999 13:22:05 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Andres Marcel Mellado Rebolledo
Subject: ASpartame????
MIME-Version: 1.0
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Hi, I would like to know if someone has listen about the toxical effects =
of consume of Aspartame??????
THANK you!!!!!
Andres M. Mellado R.
Ingeniero Civil Qu=EDmico
WebPage:
=20
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Content-Type: text/html;
charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
Hi, I would like to know if someone has listen about =
the=20
toxical effects of consume of Aspartame??????
THANK you!!!!!
Andres M. Mellado R.Ingeniero Civil =
Qu=EDmico
WebPage:
oozers.samsplace/103/index.html
------=_NextPart_000_039E_01BECBA0.5E9CE8E0--
=========================================================================
Date: Mon, 12 Jul 1999 00:24:51 EDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: FYI- Slightly different twist on Bioterrorism
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
FYI
Ed Krisiunas, MT(ASCP), CIC, MPH
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
Irish police arrest UK water plot blackmailer
By Braden Reddall
DUBLIN, July 10 (Reuters) - Irish police arrested a man on Saturday linked to
a plot to murder up to a million people by poisoning England's water supply
unless British troops were pulled out of Northern Ireland, authorities said.
Irish police said the man, in his 50s, was arrested at a Dublin address after
a month-long investigation by British and Irish police into the plot, which
involved dumping weed killer into fire hydrants.
The arrest was made as Northern Ireland headed for a crucial week in trying
to salvage last year's Good Friday peace agreement that seeks to end decades
of sectarian violence between the Protestant majority and Roman Catholic
minority.
Britain's government said it kept the threat under wraps until the man's
arrest for fear of causing public panic or encouraging ``copycat'' imitators.
Other arrests were possible.
While stressing it did not believe the plan would have been able to be fully
carried out or would have put people in serious danger, Britain's Cabinet
office said the threat ``displayed enough technical detail to give cause for
concern.''
``(Officials) concluded that even if a threat was carried out, the risk to
people including the very old and the very young was negligible,'' a
statement from the Cabinet Office said.
``The people most at risk were kidney dialysis patients who need a great
amount of water.''
In letters sent to various officials, the man claimed to be from a previously
unknown Irish republican group called ``The Republican Revenge Group'' and
demanded Britain pull its 17,000 troops out of Northern Ireland by June 18.
British officials said they believed the man was originally from Scotland.
Letters were sent to the British ambassador to Ireland, the Papal Nuncio in
Dublin and Britain's water authority. One of the three letters also said a
letter, which was never traced, was sent to Irish Prime Minister Bertie
Ahern.
The letters threatened to kill ``up to one million'' people and even gave
non-Britons a deadline to leave the United Kingdom before the threat was
carried out.
``The overriding consideration for the government, the police and the water
industry was public safety. An immediate public warning would be issued if
necessary, but there was no need to do so,'' the statement said.
British officials held an off-the-record briefing with local media about the
threat last month, but imposed a news blackout.
``Government officials and the police had concluded that public safety would
be best served if the letters were denied publicity,'' the Cabinet Office
statement said.
``Experience showed it would also give rise to hoaxes and copycat activity,''
it added.
Guerrilla violence between groups that want a united Ireland and those who
want continued British rule has plagued Northern Ireland for the past 30
years, although most groups have called ceasefires that led to last year's
peace agreement.
The troubled province now faces a July 15 deadline from British leader Tony
Blair and Ahern to implement last year's accord, which has been stalled due
to a row over when guerrilla arms should be handed over.
Pro-British Protestant politicians want the Irish Republican Army (IRA) to
give up all its weapons before its political arm Sinn Fein takes up seats in
an all-party executive and assembly aimed at giving the province more of a
say in its own affairs.
=========================================================================
Date: Mon, 12 Jul 1999 09:09:43 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Bernholc, Nicole M"
Subject: Re: ASpartame????
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
Seems like there is a lot of controversy about this one. There is an =
urban
legend site that goes into detail but many people say it is untrue. I =
would
probably say there is a measure of truth there and some people are =
effected
but not most. It effects the liver.
-----Original Message-----
From: Andres Marcel Mellado Rebolledo [mailto:ligoja9@]
Sent: Sunday, July 11, 1999 3:22 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: ASpartame????
Hi, I would like to know if someone has listen about the toxical =
effects of
consume of Aspartame??????
=20
THANK you!!!!!
=20
=20
=20
=20
=20
Andres M. Mellado R.
Ingeniero Civil Qu=EDmico
=20
WebPage:
=20
=20
=========================================================================
Date: Mon, 12 Jul 1999 09:22:42 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Dan Liberman
Subject: Re: BMBL (4th Ed.) biohaz sign
MIME-Version: 1.0
Content-Type: text/plain
Diane,
I think the point is that the statement on signage appears on page 19 and is
not repeated on page 21. All other items are repeated under both BSL1 and 2.
I believe this is a printing error.
Dan Liberman
> -----Original Message-----
> From: Diane Fleming [SMTP:Dimerck@]
> Sent: Friday, July 09, 1999 4:12 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Re: BMBL (4th Ed.) biohaz sign
>
> Just because the agent doesn't consistently (new word added in
> this
> edition) cause disease in healthy adult humans does not mean it is not
> infectious. Thus, even at BSL 1, a warning sign is appropriate. Canine
> hepatitis virus is an animal pathogen which does not include healthy adult
> humans in its host range, but it is infectious. Other BSL 1 agents can
> cause
> disease in the immunosuppressed or immunocompromised, the elderly and the
> very young, or in plants as well as animals. It seems appropriate to warn
> of
> the hazard to these groups.
>
> Regarding Chris's question: BSL 2 uses all of the practices of BSL
> !
> and the ones additionally listed for level 2 as in Table 1 (pg52 of BMBL).
> Hope that helps. Diane
> Diane O. Fleming, Ph.D., CBSP
> Biosafety Consultant
> 15611 Plumwood Court
> Bowie, MD 20716-1434
> Tel 301-249-3951
> FAX 301-249-8837
> email Dimerck@
=========================================================================
Date: Mon, 12 Jul 1999 09:57:42 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: ASpartame????
Mime-Version: 1.0
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FYI - I've attached a copy of the RTECS for Aspartame in a text document. Acute
toxicity LD50 is in the gram/kg range, oral, rodent. check it out. Judy
Pointer, UTMDACC, BSO
(See attached file: Aspartam.txt)
"Bernholc, Nicole M" on 07/12/99 08:09:43 AM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: Re: ASpartame????
Seems like there is a lot of controversy about this one. There is an urban
legend site that goes into detail but many people say it is untrue. I would
probably say there is a measure of truth there and some people are effected
but not most. It effects the liver.
-----Original Message-----
From: Andres Marcel Mellado Rebolledo [mailto:ligoja9@]
Sent: Sunday, July 11, 1999 3:22 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: ASpartame????
Hi, I would like to know if someone has listen about the toxical effects of
consume of Aspartame??????
THANK you!!!!!
Andres M. Mellado R.
Ingeniero Civil Qu
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=EDmico
WebPage:
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--0__=oJ258L9FrKHSSUZoc2oguIEXJJh8xkORhWvqjHfRSw4sFhi8TTibShfu--
=========================================================================
Date: Mon, 12 Jul 1999 10:18:00 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: food service establishment licensing
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Dear Michael:
You are entering legally dangerous territory when all communities have
public servants who are hired and certified to do food inspection
preparation and "restaurants". I strongly recommend you get out of
that end of biosafety and turn it over to the food and food service
inspection in your city county or township. The fact that you are not using
standard protocols and are unsure of inspection requirements illustrates
my arguement. All that stuff is highly standardized. Your
activities are a law suit waiting to happen in my humble opinion.
Regards,
Joe Coggin, Jr. Ph.D., CBSP, RBP, NR AAM PH&Ls and BS
On Fri, 9 Jul 1999, Mike Pascucilla wrote:
> Hello Biosafety Community:
>
> Part of our universities biological health and safety program focuses
> on foodborne illness prevention and we solely inspect all the food
> service establishments on campus, without local or state health
> dept. assistance. We are currently developing a licensing program
> and a sanitary food code. Does anyone have any information or a
> similar program on their campus? Any information will be greatly
> appreciated. Please email me directly.
>
> Thank you,
> Michael Pascucilla, R.S.
> Environmental Health and Safety
> 3102 Horsebarn Hill Rd, Box U-97
> Storrs, CT 06269
> Phone: (860)486-3613
> Fax: (860) 486-1160
> E-Mail: Mike@safety.ehs.uconn.edu
>
=========================================================================
Date: Tue, 13 Jul 1999 15:25:57 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Taylor, David G. PHD"
Subject: Re: BMBL (4th Ed.) biohaz sign
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
The 4th Ed. BMBL is on-line now. Check it out at
Dave Taylor,
CDC Office of Health and Safety
-----Original Message-----
From: Diane Fleming [mailto:Dimerck@]
Sent: Friday, July 09, 1999 5:12 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: BMBL (4th Ed.) biohaz sign
Just because the agent doesn't consistently (new word added in this
edition) cause disease in healthy adult humans does not mean it is not
infectious. Thus, even at BSL 1, a warning sign is appropriate. Canine
hepatitis virus is an animal pathogen which does not include healthy adult
humans in its host range, but it is infectious. Other BSL 1 agents can
cause
disease in the immunosuppressed or immunocompromised, the elderly and the
very young, or in plants as well as animals. It seems appropriate to warn
of
the hazard to these groups.
Regarding Chris's question: BSL 2 uses all of the practices of BSL !
and the ones additionally listed for level 2 as in Table 1 (pg52 of BMBL).
Hope that helps. Diane
Diane O. Fleming, Ph.D., CBSP
Biosafety Consultant
15611 Plumwood Court
Bowie, MD 20716-1434
Tel 301-249-3951
FAX 301-249-8837
email Dimerck@
=========================================================================
Date: Wed, 14 Jul 1999 12:18:14 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Leonard, Thomas"
Subject: Toxplasma Revisited
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Some of you may recall the lively discussion we had some time ago regarding
the interpretation of BMBL's Toxoplasma Gondii statement: "Because of the
grave consequences of toxoplasmosis in the developing fetus, serologically
negative women of childbearing age who might become pregnant should not
work with Toxoplasma in the same laboratory room where these materials are
handled."
Some points raised during the discussion were that there are circumstances
where
seronegative females may work with Toxoplasma spp. while posing minimal
risk to the fetus. In such cases, following the recommendations of BMBL
might pose unnecessary burden on research efforts. Further, because of the
uncharacteristically rigid nature of the BMBL Toxoplasma statement,
organizations who do not follow the recommendation may be placed in a
precarious legal position in the case where an incident does occur,
regardless of the circumstances involved and/or nature of the incident.
Several folks expressed discriminatory concerns as well.
I forwarded these concerns to the CDC to pass on to the BMBL editors.
Accordingly, the statement found in the 4th edition of BMBL has been
modified to: "Because of the grave consequences of toxoplasmosis in the
developing fetus, serologically negative women of childbearing age who
might become pregnant should receive extensive counseling from a
well-informed laboratory supervisor about the potential risks to the fetus.
Fully informed employees who choose not to be exposed should be provided
with alternative assignments in a work area where viable Toxoplasma
organisms are not being handled." In addition, the 4th edition provides
more detailed information on the risks associated with various stages of
parasite development and routes of exposure.
The modified statement allows greater flexibility in advising on
experiments involving T. Gondii. The new statement is, in part, a result of
the contributions and comments from the members of this list.
Regards, Tom
R. Thomas Leonard, M.S.,CSP
Safety Officer
The Wistar Institute
3601 Spruce Street
Philadelphia, PA 19104
tleonard@wistar.upenn.edu
Ph:215-898-3712
Fx:215-898-3868
=========================================================================
Date: Wed, 14 Jul 1999 13:27:03 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Betsy Gilman
Subject: Centrifuge Safety
MIME-Version: 1.0
Content-Type: multipart/alternative;
boundary="----=_NextPart_000_002C_01BECDFC.8FB7E980"
This is a multi-part message in MIME format.
------=_NextPart_000_002C_01BECDFC.8FB7E980
Content-Type: text/plain;
charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
Dear Group:
I have a researcher with a Sorvall tabletop centrifuge who would like to =
centrifuge flat tissue culture dishes (96 well plates). The =
manufacturer doesn't seem to make a carrier with aerosol containment for =
plates for this particular centrifuge, so plates would be spun in an =
open format. Since this is a BL2+ project, open plates would not be =
allowed. The researcher volunteered to parafilm the plates, and then =
put them in a plastic bag that is heat-sealed. They would be =
loaded/unloaded in a biological safety cabinet. This sounds OK, but I =
was wondering if anyone knows of any specific centrifuge manufacturers =
that may have sealed carriers for plates. Any thoughts on how this =
could be done safely would be greatly appreciated. =20
You may respond directly to me at egilman@bu.edu. Thanks in advance for =
your assistance.
Betsy Gilman
Elizabeth A. Gilman, MS, RBP, SM (NRM), CBSP
Laboratory Safety Manager / Biological Safety Officer
Office of Environmental Health & Safety
Boston University Medical Campus
617-638-8842
617-638-8822 fax
egilman@bu.edu
------=_NextPart_000_002C_01BECDFC.8FB7E980
Content-Type: text/html;
charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
Dear Group:
I have a researcher with a Sorvall tabletop =
centrifuge who=20
would like to centrifuge flat tissue culture dishes (96 well =
plates). The=20
manufacturer doesn't seem to make a carrier with aerosol containment for =
plates=20
for this particular centrifuge, so plates would be spun in an open =
format. =20
Since this is a BL2+ project, open plates would not be allowed. =20
The researcher volunteered to parafilm the plates, =
and then=20
put them in a plastic bag that is heat-sealed. They would be=20
loaded/unloaded in a biological safety cabinet. This sounds OK, =
but I was=20
wondering if anyone knows of any specific centrifuge manufacturers that =
may have=20
sealed carriers for plates. Any thoughts on how this could be done =
safely=20
would be greatly appreciated.
You may respond directly to me at egilman@bu.edu. Thanks in =
advance for=20
your assistance.
Betsy Gilman
Elizabeth A. Gilman, MS, RBP, SM =
(NRM),=20
CBSPLaboratory Safety Manager / Biological Safety OfficerOffice =
of=20
Environmental Health & SafetyBoston University Medical=20
Campus617-638-8842617-638-8822 faxegilman@bu.edu
------=_NextPart_000_002C_01BECDFC.8FB7E980--
=========================================================================
Date: Wed, 14 Jul 1999 13:16:31 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Lori Nicholson
Subject: UK position
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Our facility in Oxford England needs to be staffed with an H&S person.
PowderJect Pharmaceuticals, PLC, is seeking highly motivated individuals to
join an outstanding team. PowderJect has developed a distinctive
technological expertise focused on particle delivery of drugs for broad
potential prophylactic and therapeutic utility in delivery, infectious
diseases and cancer. The position may have limited facility as well as
health and safety type responsibilities in the beginning. The candidate
should have 3-5 years experience in H&S in a technology or research type
facility. This is not the official job description just a plea for help
on finding potential candidates. I am not sure where to even begin to
recruit such a position. I am the Corporate EH&S person but I am stationed
in the states so my normal network of professionals is not helpful. Any
suggestions on where to begin recruiting for this person would be greatly
appreciated.
Email to me directly is preferred.
Lori Nicholson
lori_nicholson@
=========================================================================
Date: Thu, 15 Jul 1999 08:32:06 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Heather H. Gonsoulin"
Subject: Formic Acid glove compatibility
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: quoted-printable
Goodmorning all,
We will begin using formic acid for histological processing in the near =
future, and I am in the process of looking for gloves to use with this =
chemical. I have been able to get samples of a few neoprene gloves, but =
they are just too thick for the task that needs to be preformed. Does =
anyone have any suggestions of gloves that they have used or know work =
well in this situation? Oh, and if they could also be used with xylene =
it would be wonderful!
Thanks for the help,
Heather
Heather H. Gonsoulin, RRA
Occupational Health and Safety Officer
USL-NIRC
( 318-482-0306 (fax) 318-373-0057
hah8377@usl.edu
=========================================================================
Date: Thu, 15 Jul 1999 09:50:29 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Re: Formic Acid glove compatibility
MIME-Version: 1.0
Content-Type: text/plain
According to Safe Skin's Chemical Resistance and Barrier Guide, Formic Acid
use with Nitrile is considered Good chemical resistance and Formic acid with
natural is considered excellent chemical resistance. You can call safe
technical affairs at this number 1-800-462-9993. The chart used to be on
the web - but I can not locate it.
Xylene information off the same chart:
nitrile is fair chemical resistance
natural is poor chemical resistance
> ----------
> From: Heather H. Gonsoulin[SMTP:hah8377@USL.EDU]
> Sent: Thursday, July 15, 1999 9:32 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Formic Acid glove compatibility
>
> Goodmorning all,
>
> We will begin using formic acid for histological processing in the near
> future, and I am in the process of looking for gloves to use with this
> chemical. I have been able to get samples of a few neoprene gloves, but
> they are just too thick for the task that needs to be preformed. Does
> anyone have any suggestions of gloves that they have used or know work
> well in this situation? Oh, and if they could also be used with xylene it
> would be wonderful!
>
> Thanks for the help,
> Heather
>
> Heather H. Gonsoulin, RRA
> Occupational Health and Safety Officer
> USL-NIRC
> ( 318-482-0306 (fax) 318-373-0057
> hah8377@usl.edu
>
=========================================================================
Date: Thu, 15 Jul 1999 09:54:17 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stefan Wagener
Subject: Glove compatibility guide
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Our office just published a very comprehensive Guide on "Selection and Usage
of Chemically-Resistant Gloves", which is accessible on our website at:
If you like it, drop Bob Ceru (bobceru@msu.edu) our chemsafety officer a
note.
I hope this helps.
Stefan Wagener, Ph.D, CBSP
Michigan State University, ORCBS
C-126 Research Complex Engineering
East Lansing, MI 48824
Phone: (517) 355-6503
Fax: (517) 353-4871
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Heather H. Gonsoulin
Sent: Thursday, July 15, 1999 9:32 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Formic Acid glove compatibility
Goodmorning all,
We will begin using formic acid for histological processing in the near
future, and I am in the process of looking for gloves to use with this
chemical. I have been able to get samples of a few neoprene gloves, but
they are just too thick for the task that needs to be preformed. Does
anyone have any suggestions of gloves that they have used or know work well
in this situation? Oh, and if they could also be used with xylene it would
be wonderful!
Thanks for the help,
Heather
Heather H. Gonsoulin, RRA
Occupational Health and Safety Officer
USL-NIRC
( 318-482-0306 (fax) 318-373-0057
hah8377@usl.edu
=========================================================================
Date: Thu, 15 Jul 1999 09:40:12 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Leslie Delpin
Subject: Re: Formic Acid glove compatibility
In-Reply-To:
Have you looked at Microgrip purple nitrile gloves distributed by
VWR? As a reference, the reorder number is 40101-348 for size large
which fit like a medium.
Leslie Delpin - Biological Health and Safety Manager
University of Connecticut
Environmental Health and Safety
3102 Horsebarn Hill Rd. Box U-97
Storrs, CT 06269-4097
(860)486-2436
(860)486-1106
lmdelpin@ehs.uconn.edu
=========================================================================
Date: Thu, 15 Jul 1999 10:08:22 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Re: Glove compatibility guide
MIME-Version: 1.0
Content-Type: text/plain
Excellent Web Site!
> ----------
> From: Stefan Wagener[SMTP:stefan@PILOT.MSU.EDU]
> Sent: Thursday, July 15, 1999 9:54 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Glove compatibility guide
>
> Our office just published a very comprehensive Guide on "Selection and
> Usage
> of Chemically-Resistant Gloves", which is accessible on our website at:
>
>
>
>
> If you like it, drop Bob Ceru (bobceru@msu.edu) our chemsafety officer a
> note.
>
> I hope this helps.
>
> Stefan Wagener, Ph.D, CBSP
> Michigan State University, ORCBS
> C-126 Research Complex Engineering
> East Lansing, MI 48824
> Phone: (517) 355-6503
> Fax: (517) 353-4871
>
>
>
>
>
> -----Original Message-----
> From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
> Behalf Of Heather H. Gonsoulin
> Sent: Thursday, July 15, 1999 9:32 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Formic Acid glove compatibility
>
>
> Goodmorning all,
>
> We will begin using formic acid for histological processing in the near
> future, and I am in the process of looking for gloves to use with this
> chemical. I have been able to get samples of a few neoprene gloves, but
> they are just too thick for the task that needs to be preformed. Does
> anyone have any suggestions of gloves that they have used or know work
> well
> in this situation? Oh, and if they could also be used with xylene it
> would
> be wonderful!
>
> Thanks for the help,
> Heather
>
> Heather H. Gonsoulin, RRA
> Occupational Health and Safety Officer
> USL-NIRC
> ( 318-482-0306 (fax) 318-373-0057
> hah8377@usl.edu
>
=========================================================================
Date: Thu, 15 Jul 1999 09:35:50 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Al Jin
Subject: Re: Formic Acid glove compatibility
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Heather,
Neoprene gloves yields a >6 hr breakthrough time when submerged in formic
acid. Nitrile gloves yields a breakthrough time between 3-4 hr when
submerged in formic acid. If you can find comfortable neoprene gloves try
looking for nitrile gloves.
Alfred Jin, BSO, IH, CBSP, M(ASCP), BSS(ASM), CM(ACM),
Hazards Control Department,
Lawrence Livermore National Laboratory,
7000 East Avenue MS-289, Livermore, CA 94550,
Phone:925 423-7385, Fax:423-1086, Jin2@
>Goodmorning all,
>
>We will begin using formic acid for histological processing in the near
>future, and I am in the process of looking for gloves to use with this
>chemical. I have been able to get samples of a few neoprene gloves, but
>they are just too thick for the task that needs to be preformed. Does
>anyone have any suggestions of gloves that they have used or know work
>well in this situation? Oh, and if they could also be used with xylene it
>would be wonderful!
>
>Thanks for the help,
>Heather
>
>Heather H. Gonsoulin, RRA
>Occupational Health and Safety Officer
>USL-NIRC
>( 318-482-0306 (fax) 318-373-0057
>hah8377@usl.edu
=========================================================================
Date: Thu, 15 Jul 1999 12:41:18 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: John Bristol
Subject: Nitrile gloves
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Biosafety friends,
Has anyone seen any hypersensitivity among their employees in relationship to
the wearing of the newer purple nitrile gloves? We are recommending nitrile
gloves over latex gloves for many activities in the biology laboratories because
of their intermittent chemical resistence. But recently we have seen some
contact dermatitis cases with these newer nitrile gloves possibly due to the
accelerators used in their manufacturing. I am wondering if any trends are
occurring.
Thanks for your assistance,
John Bristol
Manager of Environmental Health and Safety
Eisai Research Institute
Andover, MA
john_bristol@
=========================================================================
Date: Thu, 15 Jul 1999 13:11:55 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Cagle, Donald W"
Subject: Re: Nitrile gloves
We too have seen dermatitis with the purple nitriles and have warned all
user groups not to go to this newer glove (we had received samples from a
vendor). We weren't convinced it was the glove although this was certainly
our suspicion. Sounds like it was...
Donald W. Cagle, CIH
ES&H Director
Battelle
505 King Ave
Columbus, OH 43201
cagled@
> -----Original Message-----
> From: John Bristol [SMTP:John_Bristol@ERI.]
> Sent: Thursday, July 15, 1999 1:41 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Nitrile gloves
>
> Biosafety friends,
>
> Has anyone seen any hypersensitivity among their employees in relationship
> to
> the wearing of the newer purple nitrile gloves? We are recommending
> nitrile
> gloves over latex gloves for many activities in the biology laboratories
> because
> of their intermittent chemical resistence. But recently we have seen some
> contact dermatitis cases with these newer nitrile gloves possibly due to
> the
> accelerators used in their manufacturing. I am wondering if any trends
> are
> occurring.
>
> Thanks for your assistance,
>
> John Bristol
> Manager of Environmental Health and Safety
> Eisai Research Institute
> Andover, MA
> john_bristol@
=========================================================================
Date: Thu, 15 Jul 1999 13:18:22 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Michelle DeStefano
Subject: Re: Nitrile gloves
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Dear John,
I'm not sure exactly what the problem is with the purple gloves, but I can
tell you that I recently tried them (I can wear the blue ones) and was in
full allergic reaction within 20-30 min (hives)!!! There is also another
member of our lab that had a similiar reaction. The rest of the lab can
wear them with no problem.
Hope that this helps.
Michelle
At 12:41 PM 7/15/99 -0500, you wrote:
>Biosafety friends,
>
>Has anyone seen any hypersensitivity among their employees in relationship to
>the wearing of the newer purple nitrile gloves? We are recommending nitrile
>gloves over latex gloves for many activities in the biology laboratories
because
>of their intermittent chemical resistence. But recently we have seen some
>contact dermatitis cases with these newer nitrile gloves possibly due to the
>accelerators used in their manufacturing. I am wondering if any trends are
>occurring.
>
>Thanks for your assistance,
>
>John Bristol
>Manager of Environmental Health and Safety
>Eisai Research Institute
>Andover, MA
>john_bristol@
>
>
Michelle DeStefano, CBSP
CNY Research Corp
800 Irving Ave
Syracuse, NY 13212
email: destefam@
phone: (315) 477-4597
fax: (315) 476-5348
=========================================================================
Date: Thu, 15 Jul 1999 13:18:55 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Cagle, Donald W"
Subject: Re: Nitrile gloves
We too have seen dermatitis with the purple nitriles and have warned all
user groups not to go to this newer glove (we had received samples from a
vendor). We weren't convinced it was the glove although this was certainly
our suspicion. Sounds like it may have been...
Donald W. Cagle, CIH
ES&H Director
Battelle
505 King Ave
Columbus, OH 43201
cagled@
> -----Original Message-----
> From: John Bristol [SMTP:John_Bristol@ERI.]
> Sent: Thursday, July 15, 1999 1:41 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Nitrile gloves
>
> Biosafety friends,
>
> Has anyone seen any hypersensitivity among their employees in relationship
> to
> the wearing of the newer purple nitrile gloves? We are recommending
> nitrile
> gloves over latex gloves for many activities in the biology laboratories
> because
> of their intermittent chemical resistence. But recently we have seen some
> contact dermatitis cases with these newer nitrile gloves possibly due to
> the
> accelerators used in their manufacturing. I am wondering if any trends
> are
> occurring.
>
> Thanks for your assistance,
>
> John Bristol
> Manager of Environmental Health and Safety
> Eisai Research Institute
> Andover, MA
> john_bristol@
=========================================================================
Date: Thu, 15 Jul 1999 11:11:36 EDT
Reply-To: Karen=B.=Byers%SS%DFCI@eye.dfci.harvard.edu
Sender: A Biosafety Discussion List
From: Karen Byers
Subject: Re: Centrifuge Safety
MIME-Version: 1.0
Content-type: text/plain; charset=us-ascii
I had the same problem with centrifugation of plates at Biosafety Level 3. I
couldn't find a contained system for plates, either. We accept the use of
Mylar sheets to seal the plate, whichs stick down to the plate quite well. In
fact, the Mylar sticks well enough that researchers should practice removing
them first with an empty plate, or a plate with only media in the wells. The
NIH Working Safely with HIV video did a good visual on holding down the Mylar
plate with one hand, and then pulling the film off with the other.
Otherwise, predictably, splashes occur.
=========================================================================
Date: Thu, 15 Jul 1999 13:37:07 -0400
Reply-To: rubockpa@UMDNJ.EDU
Sender: A Biosafety Discussion List
From: Paul Rubock
Organization: eohss-umdnj
Subject: Re: Nitrile gloves
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Who is the manufacturer of the purple nitrile gloves in question-I'd like to inform
the people at my institution.
Thank you,
Paul Rubock
John Bristol wrote:
> Biosafety friends,
>
> Has anyone seen any hypersensitivity among their employees in relationship to
> the wearing of the newer purple nitrile gloves? We are recommending nitrile
> gloves over latex gloves for many activities in the biology laboratories because
> of their intermittent chemical resistence. But recently we have seen some
> contact dermatitis cases with these newer nitrile gloves possibly due to the
> accelerators used in their manufacturing. I am wondering if any trends are
> occurring.
>
> Thanks for your assistance,
>
> John Bristol
> Manager of Environmental Health and Safety
> Eisai Research Institute
> Andover, MA
> john_bristol@
=========================================================================
Date: Thu, 15 Jul 1999 13:22:23 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Leslie Delpin
Subject: Re: Nitrile gloves
In-Reply-To:
My staff and I have used the Microgrip Ambi purple nitrile gloves
distributed by VWR for about a year without incident.
Leslie Delpin - Biological Health and Safety Manager
University of Connecticut
Environmental Health and Safety
3102 Horsebarn Hill Rd. Box U-97
Storrs, CT 06269-4097
(860)486-2436
(860)486-1106
lmdelpin@ehs.uconn.edu
=========================================================================
Date: Thu, 15 Jul 1999 15:08:51 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Teri Lassiter
Organization: UMDNJ-NJDS
Subject: Re: Nitrile gloves
MIME-Version: 1.0
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This is a multi-part message in MIME format.
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Content-Transfer-Encoding: 7bit
I believe that the purple nitrile gloves are made by Safe Skin, however, I have been
using them successfully for over two months now, approximately 50 people use them
daily. We switched from the Safe Skin blue gloves to the purple because it was a
manufacturing change.
Teri E. Lassiter
UMDNJ-NJDS
Newark, NJ
John Bristol wrote:
> Biosafety friends,
>
> Has anyone seen any hypersensitivity among their employees in relationship to
> the wearing of the newer purple nitrile gloves? We are recommending nitrile
> gloves over latex gloves for many activities in the biology laboratories because
> of their intermittent chemical resistence. But recently we have seen some
> contact dermatitis cases with these newer nitrile gloves possibly due to the
> accelerators used in their manufacturing. I am wondering if any trends are
> occurring.
>
> Thanks for your assistance,
>
> John Bristol
> Manager of Environmental Health and Safety
> Eisai Research Institute
> Andover, MA
> john_bristol@
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n:Lassiter;Teri E.
tel;fax:973-972-2594
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adr:;;110 Bergen Street C-816;Newark;NJ;07103;
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--------------A14D4387DD2120D5299086E9--
=========================================================================
Date: Thu, 15 Jul 1999 15:38:29 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Teri Lassiter
Organization: UMDNJ-NJDS
Subject: Re: Nitrile gloves
MIME-Version: 1.0
Content-Type: multipart/mixed; boundary="------------CAEA8A988FCCE9A44EAB10BB"
This is a multi-part message in MIME format.
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Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
I believe that the purple nitrile gloves are made by Safe Skin, however, I have been
using them successfully for over two months now, approximately 50 people use them
daily. We switched from the Safe Skin blue gloves to the purple because it was a
manufacturing change.
Teri E. Lassiter
UMDNJ-NJDS
Newark, NJ
John Bristol wrote:
> Biosafety friends,
>
> Has anyone seen any hypersensitivity among their employees in relationship to
> the wearing of the newer purple nitrile gloves? We are recommending nitrile
> gloves over latex gloves for many activities in the biology laboratories because
> of their intermittent chemical resistence. But recently we have seen some
> contact dermatitis cases with these newer nitrile gloves possibly due to the
> accelerators used in their manufacturing. I am wondering if any trends are
> occurring.
>
> Thanks for your assistance,
>
> John Bristol
> Manager of Environmental Health and Safety
> Eisai Research Institute
> Andover, MA
> john_bristol@
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Content-Description: Card for Teri Lassiter
Content-Disposition: attachment;
filename="lassiter.vcf"
begin:vcard
n:Lassiter;Teri E.
tel;fax:973-972-2594
tel;work:973-973-3635
x-mozilla-html:FALSE
org:UMDNJ-NJDS;Environmental Safety
adr:;;110 Bergen Street C-816;Newark;NJ;07103;
version:2.1
email;internet:lassiter@umdnj.edu
title:Industrial Hygiene/Infection Controll Coord.
end:vcard
--------------CAEA8A988FCCE9A44EAB10BB--
=========================================================================
Date: Thu, 15 Jul 1999 16:12:07 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Cagle, Donald W"
Subject: Re: Nitrile gloves
The gloves we tried were "Boxed AMBI (TM) Purple Nitrile" -- and I believe
we got the sample from VWR.
Donald W. Cagle, CIH
ES&H Director
Battelle
505 King Ave
Columbus, OH 43201
cagled@
> -----Original Message-----
> From: Teri Lassiter [SMTP:lassiter@UMDNJ.EDU]
> Sent: Thursday, July 15, 1999 3:38 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Re: Nitrile gloves
>
> I believe that the purple nitrile gloves are made by Safe Skin, however, I
> have been
>
> using them successfully for over two months now, approximately 50 people
> use them
> daily. We switched from the Safe Skin blue gloves to the purple because
> it was a
> manufacturing change.
>
> Teri E. Lassiter
> UMDNJ-NJDS
> Newark, NJ
>
> John Bristol wrote:
>
> > Biosafety friends,
> >
> > Has anyone seen any hypersensitivity among their employees in
> relationship to
> > the wearing of the newer purple nitrile gloves? We are recommending
> nitrile
> > gloves over latex gloves for many activities in the biology laboratories
> because
> > of their intermittent chemical resistence. But recently we have seen
> some
> > contact dermatitis cases with these newer nitrile gloves possibly due to
> the
> > accelerators used in their manufacturing. I am wondering if any trends
> are
> > occurring.
> >
> > Thanks for your assistance,
> >
> > John Bristol
> > Manager of Environmental Health and Safety
> > Eisai Research Institute
> > Andover, MA
> > john_bristol@ >
=========================================================================
Date: Fri, 16 Jul 1999 09:17:19 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Custom Built Containment Units
MIME-Version: 1.0
Content-Type: text/plain
Looking for companies that will Custom Built Biological Containment Units
specifically for instrumentation.
Thank you and have a great weekend!
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Fri, 16 Jul 1999 08:48:54 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Kyle Boyett
Subject: Re: Custom Built Containment Units
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Bliss, You might want to try Certek in NC. James Grantham is the owner and
they do all types of custom built units to your specs. Their number is
1-800-542-4701. Hope this helps. K.
Kyle Boyett
Asst. Director of Biosafety
Occupational Health and Safety
University of Alabama at Birmingham
e-mail- kboyett@healthsafe.uab.edu
Phone- 205-934-2487
** Asking me to overlook a safety violation is like asking me to reduce the
value I place on YOUR life**
-----Original Message-----
From: Schlank Bliss BM [mailto:bliss.schlank@PHWILM.]
Sent: Friday, July 16, 1999 8:17 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Custom Built Containment Units
Looking for companies that will Custom Built Biological Containment Units
specifically for instrumentation.
Thank you and have a great weekend!
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Fri, 16 Jul 1999 09:16:08 -0500
Reply-To: "mkinsey@"
Sender: A Biosafety Discussion List
From: Melina Kinsey
Organization: MRI
Subject: Anaerobic chamber in BSL-3
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
I am in need of help locating a vendor who has an anaerobic chamber that can be
used in a BSL-3 facility. I would require either HEPA filtration of exhausted
air into the room or a direct connection to the existing ventilation system.
The units I have found on the internet are either gloveless (which would not
work) or do not have an adequate exhaust. One company talked about the exhaust
going through a bubble (liquid decontamination) filtration system. This
confused me. I am waiting to get more information from them.
Would a Class III BSC work just as good? I am not familiar with the
differences between an anaerobic chamber and the Class III BSC. They both seem
to be almost the same, with the exception of no oxygen in the anaerobic
chamber.
Any help you can provide me to help clear up my mind would be greatly
appreciated.
TIA
Melina Kinsey
Biosafety Officer
Midwest Research Institute
425 Volker Blvd.
Kansas City, MO 64110
(816) 753-7600 x1424
mkinsey@
=========================================================================
Date: Mon, 19 Jul 1999 16:55:24 -0300
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Roberto Aguilar Machado Santos Silva
Organization: Embrapa - Suinos e Aves
Subject: Serum Bank
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
We are organizing a Serum Bank for epidemiological studies.
Do you know computer programs?
Could I use bar code for sample identification?
Best wishes,
Roberto Aguilar M. S. Silva
Laboratory of Animal Health
EMBRAPA/CNPSA(Swine and Poultry National Research Center)
BR 153, km 110
Vila Tamandua,
CEP 89.700-000
Concordia, SC,
Brazil
E-mail (Lab.): rsilva@cnpsa.embrapa.br
E-mail (Home): rsilva@.br
Fax: 0055 49 442 8559
=========================================================================
Date: Tue, 20 Jul 1999 14:33:04 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Bernholc, Nicole M"
Subject: chemical agent and neurotoxin list as part of reporting requirem
ents
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I have been thrown into this suddenly. I have a copy of 42 cfr 72 but is
there a listing somewhere of warfare agents and neurotoxins which are
covered by this as well? Can you direct me to a site?
e.g., mustard agents, blood agents, nerve agents
Thanks
=========================================================================
Date: Tue, 20 Jul 1999 15:13:08 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Leslie Delpin
Subject: Re: chemical agent and neurotoxin list as part of reporting requ
In-Reply-To:
Hello Nicole,
try niosh or nceh or
atsdr.cx.html
Leslie Delpin - Biological Health and Safety Manager
University of Connecticut
Environmental Health and Safety
3102 Horsebarn Hill Rd. Box U-97
Storrs, CT 06269-4097
(860)486-2436
(860)486-1106
lmdelpin@ehs.uconn.edu
=========================================================================
Date: Tue, 20 Jul 1999 19:23:12 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Eric N. Cook"
Subject: Re: chemical agent and neurotoxin list as part of reporting
requirem ents
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Here is a good introductory talk given by Dr. Stephen Ostroff of the NCID.
If you are new to the Select Agent requirements, this is a good start.
Here is a link to get all of the CFRs in a searchable database online:
Section 72.6 of 42 CFR, regarding the transfer of select agents, has not
been around long (Since 97 I think) so if you have an older version of 42
CFR the select agent list will be absent. The agents are listed in Appendix
A of 42 CFR 72.6. I recently (just now) tried to look at Appendix A from
the GPO site with no luck. Although 72.6 is available, for some reason they
haven't included the all important appendix A.
I have saved the best for last though. This site has everything you need:
Eric Cook
Asst. BSO
MIT
At 02:33 PM 7/20/99 -0400, you wrote:
>I have been thrown into this suddenly. I have a copy of 42 cfr 72 but is
>there a listing somewhere of warfare agents and neurotoxins which are
>covered by this as well? Can you direct me to a site?
>
>e.g., mustard agents, blood agents, nerve agents
>
>Thanks
>
=========================================================================
Date: Wed, 21 Jul 1999 13:12:24 +0530
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Dr.Nanda kumar sarma"
Subject: HBV expressing cell line
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Dear all,
We are soon to work with a human hepatocellular carcinoma cell line
which expresses hepatitis B virions. Can anyone please comment on the
biosafety requirements, occupational hazards and safety precautions in
handling this cell line? Any information on the online resources is
welcome.
With thanks,
D. Nandakumara Sarma, Ph.D.,
R&D Centre, The Himalaya Drug Co.,
Makali, Bangalore - 562123, India.
email: dnksarma@bgl..in
=========================================================================
Date: Wed, 21 Jul 1999 08:48:49 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: HBV expressing cell line
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
HBV is a human pathogen requiring handling at biosafety level 2, i.e. lab
coat, gloves, control of aerosols, avoid use of needles. Select a
disinfectant that will kill HBV (gluteraldehyde, sodium hypochlorite).
Practice very good microbiological techniques.
As the route of transmission is via blood contact it is important that the
lab staff avoid use of sharps that could puncture the skin, cover all
breaks in their skin, wear gloves (and perhaps double glove if they have
skin wounds or rashes), lab coat sleeves should be tucked under the cuffs
of the gloves (no exposed skin). At the minimum, personnel should be
offered the HBV vaccine. Better would be make this a requirement for
working with this cell line.
At 01:12 PM 7/21/99 +0530, you wrote:
>
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Wed, 21 Jul 1999 09:47:31 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Don Callihan
Subject: Re: HBV expressing cell line
Mime-Version: 1.0
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(Embedded Don Callihan @ BDX
image moved 07/21/99 09:47 AM
to file: (Embedded image moved to file: pic05336.pcx)
pic14165.pcx)
Hello:
I'd like to add to Dr. Nanda's question and ask what biosafety issues there
might be when handling purified genomic DNA from Hepatitis B and Hepatitis C
virus. This does not include recombinant molecules, just naked DNA isolated from
intact virions. It would also be helpful to know of any publications or
experience that address any safety issues with handling purified viral nucleic
acids. I don't believe this issue is covered in either the NIH rDNA Guidelines
or in BMBL4.
As always, your collective wisdom is appreciated.
Don Callihan, Ph.D.
Biosafety Officer and Senior Clinical Microbiologist
BD Biosciences
P.O. Box 999 - MS982
Sparks, MD 21152
Telephone: 410.773.6684
"Dr.Nanda kumar sarma" on 07/21/99 03:42:24 AM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Don Callihan/BALT/BDX)
Subject: HBV expressing cell line
Dear all,
We are soon to work with a human hepatocellular carcinoma cell line
which expresses hepatitis B virions. Can anyone please comment on the
biosafety requirements, occupational hazards and safety precautions in
handling this cell line? Any information on the online resources is
welcome.
With thanks,
D. Nandakumara Sarma, Ph.D.,
R&D Centre, The Himalaya Drug Co.,
Makali, Bangalore - 562123, India.
email: dnksarma@bgl..in
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=========================================================================
Date: Wed, 21 Jul 1999 12:05:01 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Don Callihan
Subject: To Bank, or Not To Bank, That is the Question!
Mime-Version: 1.0
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(Embedded Don Callihan @ BDX
image moved 07/21/99 12:05 PM
to file: (Embedded image moved to file: pic20325.pcx)
pic15668.pcx)
Hello all you busy people:
I believe that there have been several discussions during the past year around
the issue of serum banking. I am interested in learning what standard practices
are being used so that people and institutions considering putting a serum bank
into practice have a starting point. The "how" is not addressed in BMBL4.
Here are some specific questions that immediately come to mind. Please feel free
to add relevent questions that I have missed. Please reply to me directly
if you wish. I will compile the replies I have received
by August 6 and distribute them to the entire biosafty list.
1. If your institution has a serum banking program, how long has it been in
place?
2. Roughly how many people participate?
3. How many times have you actually tested banked serum over the past 5 years?
4. How do you control/secure the serum, i.e., what function maintains the
records, what function has access, etc.
5. How is informed consent to collect blood performed? How is informed consent
to do testing performed?
6. How do you maintain chain of custody when the need to test arises? (What is
the process used to determine what samples are to be tested and how do you
maintain control of the sample(s) from when you remove them from storage to when
you receive the test report?
7. What are the pros and cons of the practice of serum banking? What situations
was it helpful? What problems did having a serum bank create?
Your collective wisdom is greatly appreciated.
Respectfully,
Don Callihan, Ph.D.
Biosafety Officer and Senior Clinical Microbiologist
BD Biosciences
P.O. Box 999 - MC982
Sparks, MD 21152
Telephone: 410.773.6684
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=========================================================================
Date: Wed, 21 Jul 1999 13:21:07 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Bernholc, Nicole M"
Subject: Re: chemical agent and neurotoxin list as part of reporting requi
rem ents
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Thank you for the sites. I had one, not the other.
Is there a site that lists chemical warefare agents other than this?
e.g. mustard gas types, etc?
Thank you
-----Original Message-----
From: Eric N. Cook [mailto:ecook@MIT.EDU]
Sent: Tuesday, July 20, 1999 7:23 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: chemical agent and neurotoxin list as part of reporting
requirem ents
Here is a good introductory talk given by Dr. Stephen Ostroff of the NCID.
If you are new to the Select Agent requirements, this is a good start.
Here is a link to get all of the CFRs in a searchable database online:
Section 72.6 of 42 CFR, regarding the transfer of select agents, has not
been around long (Since 97 I think) so if you have an older version of 42
CFR the select agent list will be absent. The agents are listed in Appendix
A of 42 CFR 72.6. I recently (just now) tried to look at Appendix A from
the GPO site with no luck. Although 72.6 is available, for some reason they
haven't included the all important appendix A.
I have saved the best for last though. This site has everything you need:
Eric Cook
Asst. BSO
MIT
At 02:33 PM 7/20/99 -0400, you wrote:
>I have been thrown into this suddenly. I have a copy of 42 cfr 72 but is
>there a listing somewhere of warfare agents and neurotoxins which are
>covered by this as well? Can you direct me to a site?
>
>e.g., mustard agents, blood agents, nerve agents
>
>Thanks
>
=========================================================================
Date: Wed, 21 Jul 1999 12:39:18 -0500
Reply-To: HawkinsL@omrf.ouhsc.edu
Sender: A Biosafety Discussion List
From: Larry Hawkins
Organization: Oklahoma Medical Research Foundation
Subject: Borna Disease Virus
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Any of you out there have PI's working with the Borna
disease virus??? If so, would be willing to share your
biosafety information on this virus?
According to the 4th edition of the BMBL it is a restricted
animal pathogen (pg-220) and I need to get a permit from the
USDA for import. I have done a internet search on this virus
and have not been able to find out what is its biosafety level.
Any one have a reference where I could find this information?
The ABSA list does not have the Borna disease virus nor its
family, Bornaviridae, listed in its risk groups.
Borna disease virus causes mental disorders in animals and may be
associated with human mood disorders and schizophrenia. That is all
I need is a release of this virus and more schizophrenics running around
Oklahoma. ( Please don't quote me on that last sentence, my own
schizophrenia made me write it.)
Any and all information would greatly be appreciated.
Thanks,
Lawrence J. Hawkins
OMRF
825 NE 13th
Oklahoma City, OK 73104
Voice: 405.271.7266
Fax: 405.271.7012
E-mail: Larry-Hawkins@omrf.ouhsc.edu
=========================================================================
Date: Thu, 22 Jul 1999 10:47:27 -0400
Reply-To: "eagleson@"
Sender: A Biosafety Discussion List
From: Eagleson Institute
Subject: CDC's 6th National Symposium on Biosafety
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
For your planning purposes we would like to announce the CDC's 6th National
Symposium on Biosafety. This symposium, sub-titled "Prudent Practices for
the New Millennium" will take place on February 6-9, 2000 at the Westin
Peachtree Plaza in Atlanta, Georgia.
Early-bird registration for this event is $475.00. Brochures will be
available the beginning of September. For more information, or to request
a brochure, please email us at eagleson@
Sincerely,
Mary Ann Sondrini
Executive Director
Eagleson Institute
P.O. Box 954
Sanford, ME 04073
(207) 490-1076
(207) 324-3869 Fax
eagleson@
=========================================================================
Date: Thu, 22 Jul 1999 13:26:23 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: HBV expressing cell line
MIME-Version: 1.0
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We here at RWJMS treat purified genomic DNA from Hep C as BSL1. It appears
to be exempt from NIH Guidelines. Also, in speaking with our virologists,
we have identified little danger from normal routes of exposure. We do,
however, register it as a "pathogen" for our internal purposes.
If anyone has any evidence to the contrary, I would be very interested.
Janice Flesher, MS
Principle Industrial Hygienist/Biosafety Officer
EOHSS - University Medical Dental School of NJ
97 Paterson St. #227
New Brunswick, NJ, 08901
(732) 235-8497 phone
(732) 235-8499 fax
fleshejk@umdnj.edu
-----Original Message-----
From: Don Callihan
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Wednesday, July 21, 1999 12:04 PM
Subject: Re: HBV expressing cell line
>
>
> (Embedded Don Callihan @ BDX
> image moved 07/21/99 09:47 AM
> to file: (Embedded image moved to file: pic05336.pcx)
> pic14165.pcx)
>
>
>
>
>
>
>Hello:
>
>I'd like to add to Dr. Nanda's question and ask what biosafety issues there
>might be when handling purified genomic DNA from Hepatitis B and Hepatitis
C
>virus. This does not include recombinant molecules, just naked DNA isolated
from
>intact virions. It would also be helpful to know of any publications or
>experience that address any safety issues with handling purified viral
nucleic
>acids. I don't believe this issue is covered in either the NIH rDNA
Guidelines
>or in BMBL4.
>
>As always, your collective wisdom is appreciated.
>
>Don Callihan, Ph.D.
>Biosafety Officer and Senior Clinical Microbiologist
>BD Biosciences
>P.O. Box 999 - MS982
>Sparks, MD 21152
>Telephone: 410.773.6684
>
>
>
>
>"Dr.Nanda kumar sarma" on 07/21/99 03:42:24 AM
>
>Please respond to A Biosafety Discussion List
>
>To: BIOSAFTY@MITVMA.MIT.EDU
>cc: (bcc: Don Callihan/BALT/BDX)
>Subject: HBV expressing cell line
>
>
>
>
>Dear all,
>
>We are soon to work with a human hepatocellular carcinoma cell line
>which expresses hepatitis B virions. Can anyone please comment on the
>biosafety requirements, occupational hazards and safety precautions in
>handling this cell line? Any information on the online resources is
>welcome.
>
>With thanks,
>
>D. Nandakumara Sarma, Ph.D.,
>R&D Centre, The Himalaya Drug Co.,
>Makali, Bangalore - 562123, India.
>email: dnksarma@bgl..in
>
>
>
>
>
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N:Flesher, MS;Janice;K
FN:Janice K Flesher, MS
ORG:University Medical Dental of NJ;EOHSS
TITLE:Principle industrial Hygienist/Biosafety Officer
TEL;WORK;VOICE:732-235-8497
TEL;HOME;VOICE:609-620-1206
TEL;WORK;FAX:732-235-8499
ADR;WORK:;;97 Paterson Street Ste 227;New Brunswick;NJ;08903;USA
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227=3D0D=3D0ANew Brunswick, NJ 08903=3D0D=3D0AUSA
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------=_NextPart_000_01B8_01BED445.CAB2CCE0--
=========================================================================
Date: Thu, 22 Jul 1999 22:55:17 -0400
Reply-To: jkeene@
Sender: A Biosafety Discussion List
From: "John H. Keene"
Subject: Re: HBV expressing cell line
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Hep.C is a Risk Group 2 virus and Section III-D-2-a and following of the NIH
Recombinant DNA Guidelines requires the use of BL2 containment for work with
these viruses and the genomic material from them. Please note that
"exemption" from the guidelines does not necessarily mean that the
material/agent is non-hazardous and therefore capable of being handled at
BL-1. An example: Exempt experiments, by definition in the guidelines,
could include transfer of genetic material between Salmonella and E. coli.
Since the Salmonella is an RG-2 agent, the experiments would be carried out
at BL-2. Don't confuse the RDNA guidelines with safety requirements. RDNA
guidelines generally are concerned with what new types of organisms we could
be creating and how to deal with those, but not with the occupational safety
aspects.
Having said this, researchers should be aware that BL-2 practices and
containment laboratories are not onerous. The BL-2 practices are really
good microbiological practices, good for both protection of personnel and of
research. Furthermore, most well constructed laboratories are constructed to
meet BL-2 requirements anyway.
Bottom line is don't confuse the purposes of RDNA Guidelines with the
purposes of the BMBL. They are not identical, nor are they mutually
exclusive.
John H. Keene, Dr. P.H., CBSP
Biohaztec Associates, Inc
Midlothian,VA
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Janice Flesher
Sent: Thursday, July 22, 1999 1:26 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: HBV expressing cell line
We here at RWJMS treat purified genomic DNA from Hep C as BSL1. It appears
to be exempt from NIH Guidelines. Also, in speaking with our virologists,
we have identified little danger from normal routes of exposure. We do,
however, register it as a "pathogen" for our internal purposes.
If anyone has any evidence to the contrary, I would be very interested.
Janice Flesher, MS
Principle Industrial Hygienist/Biosafety Officer
EOHSS - University Medical Dental School of NJ
97 Paterson St. #227
New Brunswick, NJ, 08901
(732) 235-8497 phone
(732) 235-8499 fax
fleshejk@umdnj.edu
-----Original Message-----
From: Don Callihan
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Wednesday, July 21, 1999 12:04 PM
Subject: Re: HBV expressing cell line
>
>
> (Embedded Don Callihan @ BDX
> image moved 07/21/99 09:47 AM
> to file: (Embedded image moved to file: pic05336.pcx)
> pic14165.pcx)
>
>
>
>
>
>
>Hello:
>
>I'd like to add to Dr. Nanda's question and ask what biosafety issues there
>might be when handling purified genomic DNA from Hepatitis B and Hepatitis
C
>virus. This does not include recombinant molecules, just naked DNA isolated
from
>intact virions. It would also be helpful to know of any publications or
>experience that address any safety issues with handling purified viral
nucleic
>acids. I don't believe this issue is covered in either the NIH rDNA
Guidelines
>or in BMBL4.
>
>As always, your collective wisdom is appreciated.
>
>Don Callihan, Ph.D.
>Biosafety Officer and Senior Clinical Microbiologist
>BD Biosciences
>P.O. Box 999 - MS982
>Sparks, MD 21152
>Telephone: 410.773.6684
>
>
>
>
>"Dr.Nanda kumar sarma" on 07/21/99 03:42:24 AM
>
>Please respond to A Biosafety Discussion List
>
>To: BIOSAFTY@MITVMA.MIT.EDU
>cc: (bcc: Don Callihan/BALT/BDX)
>Subject: HBV expressing cell line
>
>
>
>
>Dear all,
>
>We are soon to work with a human hepatocellular carcinoma cell line
>which expresses hepatitis B virions. Can anyone please comment on the
>biosafety requirements, occupational hazards and safety precautions in
>handling this cell line? Any information on the online resources is
>welcome.
>
>With thanks,
>
>D. Nandakumara Sarma, Ph.D.,
>R&D Centre, The Himalaya Drug Co.,
>Makali, Bangalore - 562123, India.
>email: dnksarma@bgl..in
>
>
>
>
>
=========================================================================
Date: Fri, 23 Jul 1999 06:41:21 EDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: Safety Training at ACS New Orleans - Commercial
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Division of Chemical Health & Safety Workshops
at the American Chemical Society National Meeting in New Orleans, LA
Register NOW On-Line at
* * * * Discount registration deadline = 5 August 1999 * * * *
FRIDAY, August 20, 1999
8:30 - 4:30
Westin Canal Place, River Room
$199 non-members / $169 DivCHAS members
The Laboratory Safety Workshop
Dr. Jim Kaufman
This classic presentation on laboratory safety by Dr. Kaufman has been
attended by thousands of safety professionals. With experience in both
industrial and academic laboratories, Jim gives a "real world" approach to
safety issues in the laboratory. His stories are both interesting and
pertinent, and interactive demonstrations help you deal with everything from
creative wiring in the lab to administrators without a vision of what it
means to have a safe workplace. Highly entertaining, and an excellent forum
to speak openly about safety problems in your workplace.
SATURDAY, August 21, 1999
8:30 - 4:30
Westin Canal Place, Terrace
$249 non-members / $199 DivCHAS members
How to be a More Effective Chemical Hygiene Officer
Dr. Jim Kaufman, Mr. Russell Phifer, Dr. George Wahl
Take a close look at the Chemical Hygiene Officer position, and prepare at
the same time for the CHP Certification exam to be held the next day.
Kaufman, Phifer and Wahl give a different slant to safety issues in the
laboratory, focusing on what you do and how you can do it better. The
presenters bring a wide variety of experience to the table, but the real
stars of the workshop are you - past attendees note the interactive approach
focusing on their problems, from getting administrators involved in safety
to dealing with regulatory concerns. The course covers all of the content
areas of the certification exam (presented on Sunday, August 22 through the
National Registry of Clinical Chemistry), including a sample test in the
same format as the real one. Whether you are a new Chemical Hygiene Officer
or an "old" one, you will find something to put to real use in this
fast-paced presentation.
SATURDAY, August 21, 1999
8:00 - Noon
Westin Canal Place, Ballroom I
$49 DivCHAS members / $99 non-members
Cleanup of Small Laboratory Spills - THE DIVISION FOUNDER'S WORKSHOP
Dr. Neal Langerman
This presentation will help you prepare policies on dealing with small
laboratory spills, from clarification of reporting requirements to actual
cleanup procedures. Dr. Langerman packs a great deal of information into
this half-day workshop; you will leave with ideas on preventing spills as
well as preparing for the inevitable when it occurs.
SATURDAY, August 21, 1999
1:00 - 5:00
Westin Canal Place, Ballroom I
$175 non-members / $125 DivCHAS members
Emergency Response: Contaminated Employee
Dr. Neal Langerman
Learn the best ways to deal with a potentially serious problem in a
laboratory emergency - contaminated employees. Is issue has long been
ignored, both by laboratory facilities and hospital emergency rooms. When an
employee becomes contaminated and medical attention is required, having a
policy in place will result in safer handling of the situation from all
perspectives. Make sure your procedures can help minimize problems before
the accident by attending this interesting and informative presentation.
REGISTRATION for all workshops is available online from the Division of
Chemical Health & Safety's web site -
There is also a link to
join
the Division; the cost is $29, which includes an annual subscription to
Chemical Health & Safety Magazine!
No internet access? Fax name, address, phone, workshop(s) to be attended to
(603)-462-2729.
DIVISION MEMBERS wishing to register at the member rate MUST register
no later than August 5 through the Division's web-site!
If you have any specific questions on the program, please contact Russ
Phifer by email
(rphifer@), fax (603-462-2729) or telephone (610-696-9220).
Checks for payment should be made out to "Division of Chemical Health &
Safety" and mailed to: WC Environmental, PO Box 39, West Chester, PA 19381.
Purchase orders are also accepted; payment may also be made at the door.
Walk-ins may be accepted, but space is limited for all sessions.
We hope to see you all in New Orleans! LAISSEZ LES BON TEMPS ROULEZ!!!!
George H. Wahl, Jr.
Professor of Chemistry
Box 8204
N C State University
Raleigh, NC 27695-8204
(919) 515-2941
FAX (919) 515-3757
george_wahl@ncsu.edu
=========================================================================
Date: Fri, 23 Jul 1999 08:25:36 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: Re: HBV expressing cell line
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Hepatitis C is indeed classified as BL2, but there is a difference between a
whole organism and purified genomic DNA. We need to make sure we are
evaluating the specific thing we are working with when determining risk.
According to Janet's message, they have evaluated risk aside from just the
NIH guidelines' requirements.
Julie A. Johnson, Ph.D.
Biosafety Officer
Environmental Health and Safety
Iowa State University
Ames, IA 50011
e-mail: jajohns@iastate.edu
phone: 515-294-7657
fax: 515-294-9357
web site:
-----Original Message-----
From: John H. Keene [mailto:jkeene@]
Sent: Thursday, July 22, 1999 9:55 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: HBV expressing cell line
Hep.C is a Risk Group 2 virus and Section III-D-2-a and following of the NIH
Recombinant DNA Guidelines requires the use of BL2 containment for work with
these viruses and the genomic material from them. Please note that
"exemption" from the guidelines does not necessarily mean that the
material/agent is non-hazardous and therefore capable of being handled at
BL-1. An example: Exempt experiments, by definition in the guidelines,
could include transfer of genetic material between Salmonella and E. coli.
Since the Salmonella is an RG-2 agent, the experiments would be carried out
at BL-2. Don't confuse the RDNA guidelines with safety requirements. RDNA
guidelines generally are concerned with what new types of organisms we could
be creating and how to deal with those, but not with the occupational safety
aspects.
Having said this, researchers should be aware that BL-2 practices and
containment laboratories are not onerous. The BL-2 practices are really
good microbiological practices, good for both protection of personnel and of
research. Furthermore, most well constructed laboratories are constructed to
meet BL-2 requirements anyway.
Bottom line is don't confuse the purposes of RDNA Guidelines with the
purposes of the BMBL. They are not identical, nor are they mutually
exclusive.
John H. Keene, Dr. P.H., CBSP
Biohaztec Associates, Inc
Midlothian,VA
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Janice Flesher
Sent: Thursday, July 22, 1999 1:26 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: HBV expressing cell line
We here at RWJMS treat purified genomic DNA from Hep C as BSL1. It appears
to be exempt from NIH Guidelines. Also, in speaking with our virologists,
we have identified little danger from normal routes of exposure. We do,
however, register it as a "pathogen" for our internal purposes.
If anyone has any evidence to the contrary, I would be very interested.
Janice Flesher, MS
Principle Industrial Hygienist/Biosafety Officer
EOHSS - University Medical Dental School of NJ
97 Paterson St. #227
New Brunswick, NJ, 08901
(732) 235-8497 phone
(732) 235-8499 fax
fleshejk@umdnj.edu
-----Original Message-----
From: Don Callihan
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Wednesday, July 21, 1999 12:04 PM
Subject: Re: HBV expressing cell line
>
>
> (Embedded Don Callihan @ BDX
> image moved 07/21/99 09:47 AM
> to file: (Embedded image moved to file: pic05336.pcx)
> pic14165.pcx)
>
>
>
>
>
>
>Hello:
>
>I'd like to add to Dr. Nanda's question and ask what biosafety issues there
>might be when handling purified genomic DNA from Hepatitis B and Hepatitis
C
>virus. This does not include recombinant molecules, just naked DNA isolated
from
>intact virions. It would also be helpful to know of any publications or
>experience that address any safety issues with handling purified viral
nucleic
>acids. I don't believe this issue is covered in either the NIH rDNA
Guidelines
>or in BMBL4.
>
>As always, your collective wisdom is appreciated.
>
>Don Callihan, Ph.D.
>Biosafety Officer and Senior Clinical Microbiologist
>BD Biosciences
>P.O. Box 999 - MS982
>Sparks, MD 21152
>Telephone: 410.773.6684
>
>
>
>
>"Dr.Nanda kumar sarma" on 07/21/99 03:42:24 AM
>
>Please respond to A Biosafety Discussion List
>
>To: BIOSAFTY@MITVMA.MIT.EDU
>cc: (bcc: Don Callihan/BALT/BDX)
>Subject: HBV expressing cell line
>
>
>
>
>Dear all,
>
>We are soon to work with a human hepatocellular carcinoma cell line
>which expresses hepatitis B virions. Can anyone please comment on the
>biosafety requirements, occupational hazards and safety precautions in
>handling this cell line? Any information on the online resources is
>welcome.
>
>With thanks,
>
>D. Nandakumara Sarma, Ph.D.,
>R&D Centre, The Himalaya Drug Co.,
>Makali, Bangalore - 562123, India.
>email: dnksarma@bgl..in
>
>
>
>
>
=========================================================================
Date: Fri, 23 Jul 1999 08:30:24 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Lisa Wisser
Subject: Toxin inactivation information?
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Hello List,
I am looking for specific information on inactivation of toxins - including
botulinum, ricin, and the staph enterotoxins (A&B in particular) - on
surfaces & equipment and in waste. I would appreciate any useful information
you may know of - references, web sites, etc. Please feel free to respond
directly to my e-mail address.
Thanks for your help,
Lisa Wisser
Biosafety Officer
EORM
(408)822-8124
WISSERL@
=========================================================================
Date: Fri, 23 Jul 1999 11:44:47 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Biosafety Officer Position
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
>---------------------------------------------------------------------------
--------------------------------
>
>POSITION AVAILABLE
>
>Washington University School of Medicine in St. Louis, Missouri
>
>
> Biological Safety Officer
>
>Washington University School of Medicine, one of the top five Medical
Schools in
>the country, has a challenging career opportunity available for an
experienced
>Biological Safety Officer in our Environmental Health and Safety Department.
>This position will be knowledgeable in bacterial, viral, arboviral, fungal,
>rickettsial, parasitic, prion, and recombinant DNA safety and have a strong
>understanding of current biomedical research and infection control
techniques.
>
>Responsibilities include managing programs and supervising staff in the
>biological and laboratory safety division of environmental health and safety;
>developing policies, procedures, training and educational programs to promote
>and insure biological safety in research; processing and managing grant
>applications, protocol reviews and required safety inspections; and
serving on
>environmental health and safety's emergency response team.
>
>The qualified candidate must have Master'
>
>s degree in relevant field with 7 years
>experience in biological safety preferably in a large organization. Prefer
Ph.D.
>in relevant field or advanced professional degree in related field
specializing
>in biological safety. Must be knowledgeable in biosafety issues related to
>using infected animals in research and must take call one week every 10 to 11
>weeks. Strong communication and management skills are required along with
the
>ability to manage multiple projects and deadlines.
>
>We offer an outstanding compensation and benefits package including
>medical/dental insurance, retirement annuity, and dependent tuition.
>
>Interested applicants should send resume referencing job number 991796 to:
>
> Washington University School of Medicine
> Human Resources Department
> 4480 Clayton Ave., Box 8002
> St. Louis, MO 63110
> Fax: (314) 362-4923
> medicine.wustl.edu
>
>---------------------------------------------------------------------------
-----------------------------------------
>
>---------------------- Forwarded by Bruce Backus/Facilities/Washington
>University on 07/23/99 09:58 AM ---------------------------
>
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
=========================================================================
Date: Fri, 23 Jul 1999 10:57:07 -0500
Reply-To: "mkinsey@"
Sender: A Biosafety Discussion List
From: Melina Kinsey
Organization: MRI
Subject: Re: Toxin inactivation information?
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Lisa-
I can send you a copy of "Procedures for Inactivation and Safety Containment of
Toxins", developed by Dr. Robert Wannemacher of USAMRID. It has been quite
helpful in the past for me and will answer your questions. Please post me your
fax number and I will send it to you.
Be safe,
Melina Kinsey
Biosafety Officer
Midwest Research Institute
425 Volker Blvd.
Kansas City, MO 64110
(816) 753-7600 x1424
mkinsey@
-----Original Message-----
From: Lisa Wisser [SMTP:wisserl@]
Sent: Friday, July 23, 1999 10:30 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Toxin inactivation information?
Hello List,
I am looking for specific information on inactivation of toxins - including
botulinum, ricin, and the staph enterotoxins (A&B in particular) - on
surfaces & equipment and in waste. I would appreciate any useful information
you may know of - references, web sites, etc. Please feel free to respond
directly to my e-mail address.
Thanks for your help,
Lisa Wisser
Biosafety Officer
EORM
(408)822-8124
WISSERL@
=========================================================================
Date: Fri, 23 Jul 1999 09:56:42 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Al Jin
Subject: Re: Toxin inactivation information?
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/enriched; charset="us-ascii"
Content-Transfer-Encoding: quoted-printable
Lisa & Melina,
Other good references include:
TimesU.S. Army
=46ield Manual 3-9: Potential Military Chemical/Biological Agents and
CompoundsTimes=
,
Navy Publication No. P-467, Air Force Manual 355-7 (December 12,
1990).
US. Army Defense Against Toxin Weapons, Franz,
David. U.S. Army Medical Research Institute of Infectious Diseases,
Veterinary Corps, Fort Detrick, Fredrick, Maryland, MCMR-UIZ-B.
U.S. Army-Medical Management of Biological Casualties
Handbook, U.S. Army Medical Research, Institute of
Infectious Diseases, Fort Detrick, Fredrick Maryland, second edition
(August 1996).
Alfred Jin, BSO, IH, CBSP, M(ASCP),
BSS(ASM), CM(ACM),=20
Hazards Control Department,=20
Lawrence Livermore National Laboratory,
7000 East Avenue MS-289, Livermore, CA 94550,=20
Phone:925 423-7385, Fax:423-1086,=20
Jin2@
>Hello List,
>
>I am looking for specific information on inactivation of toxins -
including
>botulinum, ricin, and the staph enterotoxins (A&B in particular) - on
>surfaces & equipment and in waste. I would appreciate any useful
information
>you may know of - references, web sites, etc. Please feel free to
respond
>directly to my e-mail address.
>
>Thanks for your help,
>
>Lisa Wisser
>Biosafety Officer
>EORM
>(408)822-8124
>WISSERL@
=========================================================================
Date: Fri, 23 Jul 1999 15:07:46 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
Comments: RFC822 error: Incorrect or incomplete address field found and
ignored.
From: Gary Cseko
Subject: Laboratory Fire Safety
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: quoted-printable
I am interested in thoughts from the group on what type of fire extinguishe=
rs are "best" for buildings which have both labs and offices. Its my =
understanding that (with the exception of Halon) ABC dry chemical =
extinguishers are the best all around extinguisher (light weight, easy to =
handle, work well on most types of fires). One disadvantage is the =
residue and problems for delicate electronic equipment. What do you =
recommend? Thanks.
Gary C. Cseko, MSPH
Safety Compliance Officer
BMSB Room B77
UNM School of Medicine
Albuquerque, NM 87131-5196
(505) 272-1492
(505) 272-8738 (Fax)
=========================================================================
Date: Fri, 23 Jul 1999 18:07:34 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: greg merkle
Organization: Wright State University
Subject: Re: Laboratory Fire Safety
MIME-version: 1.0
Content-type: text/plain; charset=us-ascii
Content-transfer-encoding: 7bit
I have been recommending to laboratory supervisors and
faculty that in their laboratory areas they place a CO2
extinguisher and in the hallway a dry chemical. The same
issues would apply to an office setting where you would have
PC's and printers set up besides people in close quarters.
The idea of initial extinguisher being CO2 is that it does
not leave any residue. The biggest problem, however, is
that the CO2 is less effective than other types. Employees
are given the opportunity to be trained on how to properly
use an extinguisher. Employees are also told that if you are
not able to put out a fire with the first extinguisher you
are not to grab another in an attempt to try again, you are
to evacute the area.
Greg Merkle
Dept. Env. Health and Safety
Wright State University
Dayton OH 45435
Gary Cseko wrote:
>
> I am interested in thoughts from the group on what type of fire extinguishers are "best" for buildings which have both labs and offices. Its my understanding that (with the exception of Halon) ABC dry chemical extinguishers are the best all around extinguisher (light weight, easy to handle, work well on most types of fires). One disadvantage is the residue and problems for delicate electronic equipment. What do you recommend? Thanks.
>
> Gary C. Cseko, MSPH
> Safety Compliance Officer
> BMSB Room B77
> UNM School of Medicine
> Albuquerque, NM 87131-5196
> (505) 272-1492
> (505) 272-8738 (Fax)
=========================================================================
Date: Fri, 23 Jul 1999 18:27:00 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: greg merkle
Organization: Wright State University
Subject: Sonication of neutrophils from human blood
MIME-version: 1.0
Content-type: text/plain; charset=us-ascii
Content-transfer-encoding: 7bit
I am working with a researcher to find a way of containing
aerosols that being generated by sonicating human blood
components. I have recommended using a containment hood, a
Class II cabinet, Class I cabinet, or a capture hood type
device. I am looking for a practical, functional and
economically feasible solution. The researcher would like
to just use the fume hood. What is out there that others
have found will work successfully.
Thanks for your input I appreciate your assistance.
Greg Merkle
Dept. Env. Health and Safety
Wright State University
Dayton OH 45435
=========================================================================
Date: Sun, 25 Jul 1999 13:21:46 PDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: JIM REIMAN
Subject: Re: Laboratory Fire Safety
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="ISO-8859-1"; X-MAPIextension=".TXT"
Content-Transfer-Encoding: quoted-printable
THAT SOUNDS LIKE A GOOD APPROACH. YOU REALLY DON'T NEED TO WORRY ABOUT =
THE ELECTRONIC GEAR MUCH. IF IT'S BURNING YOU CAN BASICALLY CONSIDER IT =
GONE. BUT IT IS IMPORTANT TO HAVE A CHOICE OF EXTINGUISHERS FOR THOSE WHO=
CAN STAY CALM AND CHOOSE THE CORRECT TOOL FOR THE PARTICULAR TYPE OF FIR=
E.
JIM REIMAN
LAB. SAFETY SERVICES
jreiman343@
----------
> I have been recommending to laboratory supervisors and
> faculty that in their laboratory areas they place a CO2
> extinguisher and in the hallway a dry chemical. The same
> issues would apply to an office setting where you would have
> PC's and printers set up besides people in close quarters.
> The idea of initial extinguisher being CO2 is that it does
> not leave any residue. The biggest problem, however, is
> that the CO2 is less effective than other types. Employees
> are given the opportunity to be trained on how to properly
> use an extinguisher. Employees are also told that if you are
> not able to put out a fire with the first extinguisher you
> are not to grab another in an attempt to try again, you are
> to evacute the area.
>
> Greg Merkle
> Dept. Env. Health and Safety
> Wright State University
> Dayton OH 45435
>
> Gary Cseko wrote:
> >
> > I am interested in thoughts from the group on what type of fire
> extinguishers are "best" for buildings which have both labs and offices=
. Its
> my understanding that (with the exception of Halon) ABC dry chemical
> extinguishers are the best all around extinguisher (light weight, easy =
to
> handle, work well on most types of fires). One disadvantage is the res=
idue
> and problems for delicate electronic equipment. What do you recommend?
> Thanks.
> >
> > Gary C. Cseko, MSPH
> > Safety Compliance Officer
> > BMSB Room B77
> > UNM School of Medicine
> > Albuquerque, NM 87131-5196
> > (505) 272-1492
> > (505) 272-8738 (Fax)
=========================================================================
Date: Mon, 26 Jul 1999 07:58:13 +0100
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stuart Thompson
Subject: Re: Sonication of neutrophils from human blood
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Fume hoods must never be used for work with biological material materials
because they are neither designed to contain the hazard nor to be
decontaminated by fumigation before change of use or routine servicing.
Best wishes
Stuart
Dr Stuart Thompson
Health & Safety Services
University of Manchester
Waterloo Place
182/184 Oxford Road
Manchester M13 9PG
tel: +44 (0)161 275 5069
fax: +44 (0)161 275 6989
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of greg merkle
Sent: 23 July 1999 23:27
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Sonication of neutrophils from human blood
I am working with a researcher to find a way of containing
aerosols that being generated by sonicating human blood
components. I have recommended using a containment hood, a
Class II cabinet, Class I cabinet, or a capture hood type
device. I am looking for a practical, functional and
economically feasible solution. The researcher would like
to just use the fume hood. What is out there that others
have found will work successfully.
Thanks for your input I appreciate your assistance.
Greg Merkle
Dept. Env. Health and Safety
Wright State University
Dayton OH 45435
=========================================================================
Date: Mon, 26 Jul 1999 08:48:34 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: HBV expressing cell line
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Dear Nanda:
Bloodborne Pathogen Law 1991: When concentrating HBV or HIV BSL-3
biosafety practices are required for aerosol containment.
Joe Coggin
On Wed, 21 Jul 1999, Dr.Nanda kumar sarma wrote:
> Dear all,
>
> We are soon to work with a human hepatocellular carcinoma cell line
> which expresses hepatitis B virions. Can anyone please comment on the
> biosafety requirements, occupational hazards and safety precautions in
> handling this cell line? Any information on the online resources is
> welcome.
>
> With thanks,
>
> D. Nandakumara Sarma, Ph.D.,
> R&D Centre, The Himalaya Drug Co.,
> Makali, Bangalore - 562123, India.
> email: dnksarma@bgl..in
>
=========================================================================
Date: Mon, 26 Jul 1999 08:53:20 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: HBV expressing cell line
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
I recommended following the 1991 BBP Law which specifies BSL-3
containment of aerosols when propagating significant quantities of the
Cells and the HBV they contain. The DNA of HBV has been reported to
infectious so I would use BSL-3 containment and practices for such work.
Joe Coggin, Ph.D., RBP, CBSP
Professor of Microbiology and Immunology
and Pathology and Chairman M&I
=========================================================================
Date: Mon, 26 Jul 1999 09:14:48 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: Re: HBV expressing cell line
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Joe,
Would you happen to have a reference for the study that reported HBV DNA to
be infectious? I'd like to have it if you do.
Julie A. Johnson, Ph.D.
Biosafety Officer
Environmental Health and Safety
Iowa State University
Ames, IA 50011
e-mail: jajohns@iastate.edu
phone: 515-294-7657
fax: 515-294-9357
web site:
-----Original Message-----
From: Joseph H. Coggin Jr. [mailto:jcoggin@JAGUAR1.USOUTHAL.EDU]
Sent: Monday, July 26, 1999 8:53 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: HBV expressing cell line
I recommended following the 1991 BBP Law which specifies BSL-3
containment of aerosols when propagating significant quantities of the
Cells and the HBV they contain. The DNA of HBV has been reported to
infectious so I would use BSL-3 containment and practices for such work.
Joe Coggin, Ph.D., RBP, CBSP
Professor of Microbiology and Immunology
and Pathology and Chairman M&I
=========================================================================
Date: Mon, 26 Jul 1999 10:07:11 EDT
Reply-To: Karen=B.=Byers%SS%DFCI@eye.dfci.harvard.edu
Sender: A Biosafety Discussion List
From: Karen Byers
Subject: Re: Sonication of neutrophils from human blood
MIME-Version: 1.0
Content-type: text/plain; charset=us-ascii
One thought on minimizing the problem: has the researcher contacted the
manufacturer of the sonicator and asked about whether a closed, cup horn type
of sonication attachment is available? Depending on the make and model, it
may be possible to replace the open probe attachment with a cup horn. A
closed tube is inserted in a water bath in the "horn" probe.
Another option for "closed" sonication I have seen are Brinkmann waterbath
type systems -- I believe they are designed for ultrasonic cleaning. These
are commonly used in vaccinia research, and not very expensive.
=========================================================================
Date: Mon, 26 Jul 1999 09:20:51 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Cheri Marcham
Subject: Biosafety Level ?
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
OK group - anyone know what happened to the Health Canada Office of
Biosafety MSDS site? I have been trying for several days to connect and get
no response.
Since that resource is unavailable to me, can anyone tell me the appropriate
biosafety level for the following organisms? They are not in the BMBL that
I can tell.
Burkholderia gladioli
Alcaligenes xylosoxidans
Stenotrophomonas maltophilia
Ralstonia peckettii
Thanks.
Cheri Marcham
The University of Oklahoma Health Sciences Center
cheri-marcham@ouhsc.edu
(405) 271-3000
FAX (405) 271-1606
=========================================================================
Date: Mon, 26 Jul 1999 09:47:57 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: HBV expressing cell line
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Julie:
I recall reading that the completed DNA double stranded DNA genomic
component in Dane paticles could be extracted and purified from one type
of the three virion-like structures present in chronic hepatitis patients
had a 42 nM core component which could produce fully infectious virus
particles when transfected to several human hepatic cell lines. One of
the references in chapter 35 of the text Medical Microbiology 21st
edition Jawetz, Melnick, and Adelberg's Lange Publisher Stamford Conn. gave
the reference as I recall. I also heard at a meeting several years ago
that the RNA intermediate could be use to transfect liver cells. I 'll
look through my notes when I get back into the office next week and see
if I can find the specific reference. I would handle the prep of any HBV
DNA or RNA containing defective cores etc as BSL-2 at all times to be safe.
Regards.
More later if I can find it. Joe Coggin.
On Mon, 26 Jul 1999, Johnson, Julie A. wrote:
> Joe,
> Would you happen to have a reference for the study that reported HBV DNA to
> be infectious? I'd like to have it if you do.
>
> Julie A. Johnson, Ph.D.
> Biosafety Officer
> Environmental Health and Safety
> Iowa State University
> Ames, IA 50011
> e-mail: jajohns@iastate.edu
> phone: 515-294-7657
> fax: 515-294-9357
> web site:
>
>
>
> -----Original Message-----
> From: Joseph H. Coggin Jr. [mailto:jcoggin@JAGUAR1.USOUTHAL.EDU]
> Sent: Monday, July 26, 1999 8:53 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Re: HBV expressing cell line
>
>
> I recommended following the 1991 BBP Law which specifies BSL-3
> containment of aerosols when propagating significant quantities of the
> Cells and the HBV they contain. The DNA of HBV has been reported to
> infectious so I would use BSL-3 containment and practices for such work.
>
> Joe Coggin, Ph.D., RBP, CBSP
> Professor of Microbiology and Immunology
> and Pathology and Chairman M&I
>
=========================================================================
Date: Mon, 26 Jul 1999 10:49:11 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Brenda Barry
Subject: Re: Biosafety Level ?
MIME-Version: 1.0
Content-Type: text/plain
Cheri,
Xanthomonas (Stenotrophomonas) maltophilia was formerly classified as
Pseudomonas maltophilia (Manual of Clinical Microbiology, 6th ed. p.
522). One researcher recently proposed working with these bacteria and
our Committee on Microbiological Safety agreed that BL-2 conditions
were appropropriate for the project. I hope this helps.
Brenda Barry, Ph.D.
Biosafety Officer
Harvard Institutes of Medicine
> ----------
> From: Cheri Marcham[SMTP:Cheryl-Marcham@OUHSC.EDU]
> Sent: Monday, July 26, 1999 10:20 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Biosafety Level ?
>
> OK group - anyone know what happened to the Health Canada Office of
> Biosafety MSDS site? I have been trying for several days to connect
> and get
> no response.
>
> Since that resource is unavailable to me, can anyone tell me the
> appropriate
> biosafety level for the following organisms? They are not in the BMBL
> that
> I can tell.
>
> Burkholderia gladioli
> Alcaligenes xylosoxidans
> Stenotrophomonas maltophilia
> Ralstonia peckettii
>
> Thanks.
>
> Cheri Marcham
> The University of Oklahoma Health Sciences Center
> cheri-marcham@ouhsc.edu
> (405) 271-3000
> FAX (405) 271-1606
>
=========================================================================
Date: Mon, 26 Jul 1999 10:53:55 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Gill Norton
Organization: University of Western Ontario
Subject: Re: Biosafety Level ?
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
The Health Canada web site appears to be OK from here. Check your web
address it may be incorrect.>
Cheri Marcham wrote:
>
> OK group - anyone know what happened to the Health Canada Office of
> Biosafety MSDS site? I have been trying for several days to connect and get
> no response.
>
> Since that resource is unavailable to me, can anyone tell me the appropriate
> biosafety level for the following organisms? They are not in the BMBL that
> I can tell.
>
> Burkholderia gladioli
> Alcaligenes xylosoxidans
> Stenotrophomonas maltophilia
> Ralstonia peckettii
>
> Thanks.
>
> Cheri Marcham
> The University of Oklahoma Health Sciences Center
> cheri-marcham@ouhsc.edu
> (405) 271-3000
> FAX (405) 271-1606
--
------------------------------------------------------------------
Gillian Norton
Biosafety Officer
The University of Western Ontario
Occupational Health and Safety
Somerville House, Rm 2345
Phone: (519)661-2036
FAX: (519)661-3420
-------------------------------------------------------------------
=========================================================================
Date: Mon, 26 Jul 1999 09:54:21 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: Re: Biosafety Level ?
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I have used this site several times over the last few days. Maybe you had
an old web address?
-----Original Message-----
From: Cheri Marcham [mailto:Cheryl-Marcham@OUHSC.EDU]
Sent: Monday, July 26, 1999 9:21 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Biosafety Level ?
OK group - anyone know what happened to the Health Canada Office of
Biosafety MSDS site? I have been trying for several days to connect and get
no response.
Since that resource is unavailable to me, can anyone tell me the appropriate
biosafety level for the following organisms? They are not in the BMBL that
I can tell.
Burkholderia gladioli
Alcaligenes xylosoxidans
Stenotrophomonas maltophilia
Ralstonia peckettii
Thanks.
Cheri Marcham
The University of Oklahoma Health Sciences Center
cheri-marcham@ouhsc.edu
(405) 271-3000
FAX (405) 271-1606
=========================================================================
Date: Mon, 26 Jul 1999 08:06:34 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Sandra Fry
Subject: Re: ASpartame????
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Hi!
This is an automatic mail reponse.
I am out of the office until July 22, 1999 and will reply to your e-mail when I return.
=========================================================================
Date: Mon, 26 Jul 1999 11:42:28 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Biosafety Level ?
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Cheri,
>Burkholderia gladioli
Used to be Ps. gladioli, a plant (guess which one) pathogen. I have not
heard of causing any human or animal disease. I would rate it as BL1.
>Alcaligenes xylosoxidans
Causes a good deal of infections in debilitated hospitalized patients, thus
an opportunistic infectious agent. If you are working with a few plates or
unconcentrated overnights, BL1, if, on the other hand you are working with
high numbers or concentrated organism, BL2 would be appropriate. If the
worker(s) have any underlying immunocompromization, BL2 to BL2+.
>Stenotrophomonas maltophilia
Used to be Ps. maltophilia, a very common environmental organism that like
A. xylosoxidans is an opportunistic pathogen. See above for recommendations.
>Ralstonia peckettii
Again, another ex-Pseudomonad (species should be pickettii). Another
opportunistic pathogen, see above.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Tue, 27 Jul 1999 10:23:44 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: safety coordinator position
MIME-Version: 1.0
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this format, some or all of this message may not be legible.
------_=_NextPart_000_01BED844.0737DD70
Content-Type: text/plain;
charset="iso-8859-1"
I was asked to post this position description:
Julie A. Johnson, Ph.D.
Biosafety Officer
Environmental Health and Safety
Iowa State University
Ames, IA 50011
e-mail: jajohns@iastate.edu
phone: 515-294-7657
fax: 515-294-9357
web site:
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------_=_NextPart_000_01BED844.0737DD70--
=========================================================================
Date: Tue, 27 Jul 1999 11:13:19 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: safety coordinator position
MIME-Version: 1.0
Content-Type: multipart/mixed; boundary="----_=_NextPart_000_01BED84A.F1AE5A2C"
This message is in MIME format. Since your mail reader does not understand
this format, some or all of this message may not be legible.
------_=_NextPart_000_01BED84A.F1AE5A2C
Content-Type: text/plain;
charset="iso-8859-1"
I apologize for posting this twice, but it has been slightly modified from
the first post.
> -----Original Message-----
> From: Johnson, Julie A.
> Sent: Tuesday, July 27, 1999 10:24 AM
> To: BIOSAFTY (E-mail)
> Subject: safety coordinator position
>
> I was asked to post this position description:
>
>
>
> Julie A. Johnson, Ph.D.
> Biosafety Officer
> Environmental Health and Safety
> Iowa State University
> Ames, IA 50011
> e-mail: jajohns@iastate.edu
> phone: 515-294-7657
> fax: 515-294-9357
> web site:
>
------_=_NextPart_000_01BED84A.F1AE5A2C
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------_=_NextPart_000_01BED84A.F1AE5A2C--
=========================================================================
Date: Tue, 27 Jul 1999 11:15:24 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: safety coordinator position
MIME-Version: 1.0
Content-Type: multipart/mixed; boundary="----_=_NextPart_000_01BED84B.3E7A5928"
This message is in MIME format. Since your mail reader does not understand
this format, some or all of this message may not be legible.
------_=_NextPart_000_01BED84B.3E7A5928
Content-Type: text/plain;
charset="iso-8859-1"
Sorry to post this twice, but here has been a slight modification in the
description from the first post:
Julie A. Johnson, Ph.D.
Biosafety Officer
Environmental Health and Safety
Iowa State University
Ames, IA 50011
e-mail: jajohns@iastate.edu
phone: 515-294-7657
fax: 515-294-9357
web site:
Julie A. Johnson, Ph.D.
Biosafety Officer
Environmental Health and Safety
Iowa State University
Ames, IA 50011
e-mail: jajohns@iastate.edu
phone: 515-294-7657
fax: 515-294-9357
web site:
------_=_NextPart_000_01BED84B.3E7A5928
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name="Safety Coordinator.doc"
Content-Transfer-Encoding: base64
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filename="Safety Coordinator.doc"
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------_=_NextPart_000_01BED84B.3E7A5928--
=========================================================================
Date: Tue, 27 Jul 1999 09:49:07 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Wolz, Sarah"
Subject: VRSA
MIME-Version: 1.0
Content-Type: text/plain
Dear Group:
I have been asked by some local colleagues about working with vancomycin
resistant genes:
A team wants to clone the vancomycin resistance genes from Enterococcus
faecium into Staphylococcus aureus. The resulting strain will be an
intermediate in constructing a reporter system--the final system will not be
vancomycin-resistant. The Staph strain is one known to be avirulent in
animal (mice) systems. (I am suggesting they put some other "lethal
mutation" in this intermediate strain (an amino acid requirement or
something like that)).
Thankfully (whew), they are seeking biosafety help before going ahead with
their plans, so I'm anxious to help them out in a positive, albeit prudent
way. I'd be interested in hearing from any of you who've had experience
with MRSA or VRSA research, particularly in areas of containment, work
practices, any medical surveillance procedures, etc.
Sarah Wolz, EH&S Mgr.
PathoGenesis Corp.
swolz@
206-505-2423
=========================================================================
Date: Tue, 27 Jul 1999 11:09:03 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Wolz, Sarah"
Subject: VRSA
MIME-Version: 1.0
Content-Type: text/plain
Just to clarify a little with regards to my previous email regarding cloning
efforts with vancomycin resistance--
the group who contacted me doesn't plan to clone the entire VRE-gene, but
just to pull out the promotor region. I still have serious concerns and
plan to gather as much information from sources such as this list in order
to give them a carefully thought out answer.
Sarah
=========================================================================
Date: Tue, 27 Jul 1999 14:39:05 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: VRSA
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Sarah, I think this is the type of question I would take to my IBC members. As
a biosafety specialist, I feel qualified to question these things, but do not
consider myself a molecular biologist, and not solely qualified to answer them.
I think this type of thing needs to be reviewed by molecular biologists and
clinical microbiologists, not just biosafety people.
My first guess would be, if it's just a promoter gene OK, don't worry too much -
but the rDNA guide states in Section III-A-1-a. "The deliberate transfer of a
drug resistance trait to microorganisms that are not known to acquire the trait
naturally (see Section V-B, Footnotes and References of Sections I-IV), if such
acquisition could compromise the use of the drug to control disease agents in
humans, veterinary medicine, or agriculture, will be reviewed by RAC".
Therefore, I think a team of scientists needs to review it to see if that is a
possibility in the intermediate strain you refer to. If they think it might be,
it should be refered to ORDA for their review.
If you don't have an IBC at your shop, I'd be glad to ask some of our PhDs and
MDs that are adept at gene insert function/result predicting. Knowing my group
though, they'll probably want more information than what you just gave - like
gene maps, etc. Let me know and I'll pass your question around and send you
their responses or ask them to talk with you directly.
Judy Pointer, CBSP
MD Anderson Cancer Center
Houston, Tx
jpointer@
"Wolz, Sarah" on 07/27/99 11:49:07 AM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: VRSA
Dear Group:
I have been asked by some local colleagues about working with vancomycin
resistant genes:
A team wants to clone the vancomycin resistance genes from Enterococcus
faecium into Staphylococcus aureus. The resulting strain will be an
intermediate in constructing a reporter system--the final system will not be
vancomycin-resistant. The Staph strain is one known to be avirulent in
animal (mice) systems. (I am suggesting they put some other "lethal
mutation" in this intermediate strain (an amino acid requirement or
something like that)).
Thankfully (whew), they are seeking biosafety help before going ahead with
their plans, so I'm anxious to help them out in a positive, albeit prudent
way. I'd be interested in hearing from any of you who've had experience
with MRSA or VRSA research, particularly in areas of containment, work
practices, any medical surveillance procedures, etc.
Just to clarify a little with regards to my previous email regarding cloning
efforts with vancomycin resistance--
the group who contacted me doesn't plan to clone the entire VRE-gene, but
just to pull out the promotor region. I still have serious concerns and
plan to gather as much information from sources such as this list in order
to give them a carefully thought out answer.
Sarah Wolz, EH&S Mgr.
PathoGenesis Corp.
swolz@
206-505-2423
=========================================================================
Date: Tue, 27 Jul 1999 15:31:08 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Madeline J. Dalrymple"
Subject: Human Blood in Micro Class
MIME-version: 1.0
Content-type: text/plain; charset="iso-8859-1"
Hi there
An instructor for a general microbiology laboratory wants students
to experience making blood smears and staining, and typing blood. They
tried using bovine and sheep blood but saw only red blood cells in the
stained smears. They want students to see different types of cells. They
tried typing sheep and bovine blood with human reagents because they learned
that animal blood typing is performed on plasma. And it didn't work. So
they want to use human blood. I suggested obtaining expired blood from a
blood bank as I didn't have any other animal models to suggest.
Do you have alternative suggestions allowing students to practice
staining or typing while minimizing their risks?
Thank you
Madeline
Madeline Dalrymple
Biological Safety Officer
University of Wyoming Environmental Health and Safety Office
Laramie, Wyoming, USA
307-766-2723, fax 307-766-5678
dalrympl@uwyo.edu
=========================================================================
Date: Tue, 27 Jul 1999 15:53:32 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: Re: Human Blood in Micro Class
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I understand that you wish to be cautious in the source of human blood. But
I also see this as a teaching moment. If these students are going to pursue
careers in micro and/or infectious disease, whether as laboratorians or
researchers or nurses or MDs, then when should they become aware of the
BBP/OPIM they may be exposed to and the appropriate precautions? Perhaps
that is too much to ask of this instructor and this class.
A blood bank is one alternative and potentially the best. In the military
training I did, we used our own blood. However, this instructor may want to
have some knowns and unknowns to teach them about the variety of typing
issues and cells.
Terry Stinnett
-----Original Message-----
From: Madeline J. Dalrymple [ mailto:Dalrympl@UWYO.EDU
]
Sent: Tuesday, July 27, 1999 3:31 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Human Blood in Micro Class
Hi there
An instructor for a general microbiology laboratory wants students
to experience making blood smears and staining, and typing blood. They
tried using bovine and sheep blood but saw only red blood cells in the
stained smears. They want students to see different types of cells. They
tried typing sheep and bovine blood with human reagents because they learned
that animal blood typing is performed on plasma. And it didn't work. So
they want to use human blood. I suggested obtaining expired blood from a
blood bank as I didn't have any other animal models to suggest.
Do you have alternative suggestions allowing students to practice
staining or typing while minimizing their risks?
Thank you
Madeline
Madeline Dalrymple
Biological Safety Officer
University of Wyoming Environmental Health and Safety Office
Laramie, Wyoming, USA
307-766-2723, fax 307-766-5678
dalrympl@uwyo.edu
=========================================================================
Date: Tue, 27 Jul 1999 15:13:40 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Terri Hellman
Subject: Re: Human Blood in Micro Class
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Many moons ago (mid 1980s) when I took Micro we did the same exercise but used
our own blood. Just make it part of the exercise that you use proper decon
procedures for the slides, if your are not going to use disposables, and have
the instructor talk a bit about BBP. BBP is good basic knowledge no matter what
field these folks go into.
Madeline J. Dalrymple wrote:
> Hi there
> An instructor for a general microbiology laboratory wants students
> to experience making blood smears and staining, and typing blood. They
> tried using bovine and sheep blood but saw only red blood cells in the
> stained smears. They want students to see different types of cells. They
> tried typing sheep and bovine blood with human reagents because they learned
> that animal blood typing is performed on plasma. And it didn't work. So
> they want to use human blood. I suggested obtaining expired blood from a
> blood bank as I didn't have any other animal models to suggest.
>
> Do you have alternative suggestions allowing students to practice
> staining or typing while minimizing their risks?
>
> Thank you
>
> Madeline
>
> Madeline Dalrymple
> Biological Safety Officer
> University of Wyoming Environmental Health and Safety Office
> Laramie, Wyoming, USA
> 307-766-2723, fax 307-766-5678
> dalrympl@uwyo.edu
=========================================================================
Date: Tue, 27 Jul 1999 13:34:20 -1000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Hubert B Olipares
Subject: IND BIOSAFTY
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
IND BIOSAFTY
==============================================================================
Hubert B. Olipares, RBP
Biological Safety Officer
University of Hawaii
Environmental Health and Safety Office
2040 East-West Road
Honolulu, Hawaii 96822-2022
Telephone: 808-956-3197
Fax: 808-956-3205
E-Mail: olipares@hawaii.edu
=========================================================================
Date: Tue, 27 Jul 1999 19:47:44 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: greg merkle
Organization: Wright State University
Subject: Any known adverse exposures to sonicated blood
MIME-version: 1.0
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Content-transfer-encoding: 7bit
I am looking for information on adverse exposures or health
risks to the sonication of human blood or components when
using a class I or II biological safety cabinet that is
vented back into the lab area. I am in a discussion with a
faculty member that has concerns over the exhaust HEPA
filter not being 100% efficent in the removal of
(potentially) harmful material. The faculty person has
expressed concern that the laboratory personnel are being
placed at an unnecessary risk. Is there a need to be
concerned and demand that a Class I or II BSC be installed
with a duct to the outside? I am trying to find resources
to resolve this without it having to go to the full IBC for
a committee decision if it is not necessary to do so.
Thanks for your help
=========================================================================
Date: Wed, 28 Jul 1999 08:14:12 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
In-Reply-To:
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Brian,
Judging from the response I think you sent your message to
listserv@mitvma.mit.edu instead of biosafty@mitvma.mit.edu
Messages to the biosafty list must go to biosafty@mitvma.mit.edu
If you wish to set nomail, change other parameters of your subscription,
you send those commands to listserv@mitvma.mit.edu
>Unknown command - "I". Try HELP.
>
>> Thanks,
>Unknown command - "THANKS,". Try HELP.
>
>> Brian McShane, CIH
>Unknown command - "BRIAN". Try HELP.
>
>Summary of resource utilization
>-------------------------------
> CPU time: 0.018 sec Device I/O: 0
> Overhead CPU: 0.005 sec Paging I/O: 2
> CPU model: 9121 DASD model: 3390
>
>
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
=========================================================================
Date: Wed, 28 Jul 1999 08:36:51 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Human Blood in Micro Class
In-Reply-To:
Mime-Version: 1.0
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Madeline,
The least risky way to type human blood is for each student to use his/her
own blood. If they want to use animal blood, you will need to get reagent
kit for that animal. Check with a veterinary clinic for where to obtain
the reagents.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Wed, 28 Jul 1999 08:51:18 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Any known adverse exposures to sonicated blood
In-Reply-To:
Mime-Version: 1.0
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Sonication is well known to create aerosols. Aerosols of infectious agents
have been known to cause laboratory aquired infections. So let's look at
the experimental system that has been proposed:
If the cabinet has been recently certified, has not been moved or damaged
since that certification and the pressure drop across the filter is within
its normal range, then the probability is quite hight that the cabinet is
functioning correctly. That being the case, and assuming correct working
procedures in the cabinet, the chance of something escaping out of the
front of the cabinet is quite small (on the order of 1:1,000,000). The
HEPA worst capture rate of 99.97% is for a 0.3 micron particle. The
capture rate increases for particles smaller and larger than 0.3. So the
chance that an infectious agent makes it through a HEPA and is inhaled,
while still viable, is very, very small. Which brings us to the next part
of the risk assessment. Are the agents potentially present infectious via
an aerosol route? Will the agents be present in high enough concentration
to be an infectious dose via an aerosol? The answers are: 1) in the
concentrations normally present in the blood, the agents do not have an
aerosol route of infection; 2) unless the agents are concentrated and then
aerosolized it is extremely unlikely that you would have an infectious dose
present in the air. The final question, could the escaped agents deposit
on a surface in sufficient concentration to present an infectious threat
through direct contact? Exceedingly unlikely that enough organisms would
escape a HEPA and deposit on a surface to be a threat.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Wed, 28 Jul 1999 08:50:10 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Human Blood in Micro Class
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Try dogs blood.
When I was in the Air Force (many many, moons ago), we used to get samples
from the research groups and we did, "doggy diffs". The cell morphology is
different, but recognizable.
I would not recommend human blood because now we must deal with a
bloodborne pathogens situation. The instructors must be covered by this
law. It would be insane to hold the teachers to one standard of protection
and offer none to the students. Here we would insist that the students
also adhere to the bbp standard. We cannot afford otherwise. For the
students it is not an OSHA issue but a liability issue. And it can be
huge.
Bob
>Hi there
> An instructor for a general microbiology laboratory wants students
>to experience making blood smears and staining, and typing blood. They
>tried using bovine and sheep blood but saw only red blood cells in the
>stained smears. They want students to see different types of cells. They
>tried typing sheep and bovine blood with human reagents because they learned
>that animal blood typing is performed on plasma. And it didn't work. So
>they want to use human blood. I suggested obtaining expired blood from a
>blood bank as I didn't have any other animal models to suggest.
>
> Do you have alternative suggestions allowing students to practice
>staining or typing while minimizing their risks?
>
>Thank you
>
>Madeline
>
>Madeline Dalrymple
>Biological Safety Officer
>University of Wyoming Environmental Health and Safety Office
>Laramie, Wyoming, USA
>307-766-2723, fax 307-766-5678
>dalrympl@uwyo.edu
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Wed, 28 Jul 1999 07:58:01 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Kyle Boyett
Subject: Re: Any known adverse exposures to sonicated blood
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Greg, One of the things you probably need to do is explain to the individual
what exactly HEPA means and how its quantified. This will probably relieve
some of his concerns. Also, you may want to describe the methods of
filtration i.e.. sieving, electrostatic, mechanical, etc... Explain that
since HEPAs are rated at 99.97-99.99% efficient at 0.3 micrometers (the most
difficult size to filter) and that theoretically nothing can be 100%
efficient and still have air pass through it, that he should not be
concerned as long as the filters are tested according to NSF 49. Hope this
helps.
Kyle Boyett
Asst. Director of Biosafety
Occupational Health and Safety
University of Alabama at Birmingham
e-mail- kboyett@healthsafe.uab.edu
Phone- 205-934-2487
** Asking me to overlook a safety violation is like asking me to reduce the
value I place on YOUR life**
-----Original Message-----
From: greg merkle [mailto:greg.merkle@WRIGHT.EDU]
Sent: Tuesday, July 27, 1999 6:48 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Any known adverse exposures to sonicated blood
I am looking for information on adverse exposures or health
risks to the sonication of human blood or components when
using a class I or II biological safety cabinet that is
vented back into the lab area. I am in a discussion with a
faculty member that has concerns over the exhaust HEPA
filter not being 100% efficent in the removal of
(potentially) harmful material. The faculty person has
expressed concern that the laboratory personnel are being
placed at an unnecessary risk. Is there a need to be
concerned and demand that a Class I or II BSC be installed
with a duct to the outside? I am trying to find resources
to resolve this without it having to go to the full IBC for
a committee decision if it is not necessary to do so.
Thanks for your help
=========================================================================
Date: Wed, 28 Jul 1999 08:11:41 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: Any known adverse exposures to sonicated blood
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
I had to resend to list instead of direct. Sorry
---------------------- Forwarded by Judy M. Pointer/MDACC on 07/28/99 08:12 AM
---------------------------
Judy M. Pointer
07/28/99 08:07 AM
To: Greg merkle@WRIGHT.EDU@internet
cc:
Subject: Re: Any known adverse exposures to sonicated blood (Document link not
converted)
I hopes this reaches you Greg b/c I wasn't to sure of your e-mail address. I
don't have a reference for you but perhaps the investigator will accept my
explanation.
All microbial agents are particulate substances - not volatile molecules. Blood
should not contain any volatile components at all - unless something highly
unusual happened to the person from which it was drawn. The HEPA filter is the
most efficient filter available for particulate substances. While no filter is
100% efficient, these filters come close enough to ensure that microbs that
might be in a blood sample will be trapped. Even if 1 - 3 in 1000 (i.e. 99.97%
efficiency) aerosolized blood particles passed the HEPA and recirculated in the
lab, any piggy-backed microbs leaking through would probably have to be
infectious by inhalation to do any harm, even if they were present in sufficient
quantity to cause an infection. Blood does not typically contain microbs
infectious by this route of transmission, ergo the Blood Borne Pathogen Standard
which concentrates on contact (not aerosol transmission) precautions.
At MDA, Class IIA BSCs are considered sufficient for handling aerosolized
particulate hazards and I would allow it. These cabinets all exhaust through
HEPA filters and recirc back into the room. I only require exhausted cabinets
(Class IIB) in certain cases such as:
- when volatile agents are used in conjunction with microbs/tissue culture.
Volatile agents (as molecules in air) are not removed by HEPA filtration.
- when carcinogens are used in conjunction with microbs/tissue culture (this is
b/c of that OSHA standard that says all carcinogens must be handled in exhausted
cabinets
>The least risky way to type human blood is for each student to use his/her
>own blood. If they want to use animal blood, you will need to get reagent
>kit for that animal. Check with a veterinary clinic for where to obtain
>the reagents.
>
>
>Richard Fink, SM(NRM), CBSP
>Assoc. Biosafety Officer
>Mass. Inst. of Tech.
>617-258-5647
>rfink@mit.edu
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Wed, 28 Jul 1999 14:03:26 +0000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: BRIAN MCSHANE
Subject: Shedding of Adenovirus, Streptozotocin and Cyclosporin
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Content-Type: text/plain; charset="iso-8859-1"
Hi,
Does anyone have information concerning the shedding (or biological half =
life) of adenovirus, streptozotocin and cyclosporin by lab rats and/or =
mice. Right now all I have is anecdotal information concerning these =
issues and I'm looking for some reference material, papers, etc. Failing =
that, any information on how these are treated in your facilities would be =
of great help. Thanks.
Brian McShane, CIH
Regeneron Pharmaceuticals
brian.mcshane@
=========================================================================
Date: Wed, 28 Jul 1999 14:45:19 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Cheri Marcham
Subject: ATCC recombinant DNA course
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ATCC has an advertisement for a 5 day course entitled, "RECOMBINANT DNA:
Techniques and Applications" put on by IB3 / George Mason University -
Manassas, VA on October 4 -8, 1999. Course description says, "A five-day,
laboratory-intensive, course that covers up-to-date recombinant DNA
procedures and methodologies both in theory and in practice. This workshop
provides introductory and intermediate level instruction in
recombinant DNA laboratory techniques. A basic knowledge of nucleic acids is
helpful but not necessary." See
Do any of you know anything about this particular course? Would I be
completely lost? I have the responsibility of initially reviewing the
recombinant DNA protocol for safety and compliance issues on our campus
before going to the IBC for committee approval and am trying desperately to
find some training to educate me on what it is the researchers are doing so
I can understand their proposals. This field is so full of acronyms I
literally have to call the researcher to ask what the lingo in their
proposal means.
That's why I was hoping the Eagleson Institute would hold their "Biological
Safety Issues in the Development and Use of Recombinant DNA Gene Delivery
Technologies" course again, but in the event they do not, I am still
looking.
Ideas?
Cheri Marcham, CIH, CSP, CHMM
The University of Oklahoma Health Sciences Center
cheri-marcham@ouhsc.edu
(405) 271-3000
FAX (405) 271-1606
=========================================================================
Date: Wed, 28 Jul 1999 17:12:32 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Betsy Gilman
Subject: Re: ATCC recombinant DNA course
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Content-Transfer-Encoding: 7bit
Dear Group:
In response to Cheri's inquiry about a basic recombinant DNA course, I want
to let everyone know that the ABSA Training & Education Committee will offer
a preconference course on molecular biology and rDNA at the October ABSA
Conference. The course is titled "The Science, Techniques, And Jargon of
Molecular Biology" and will be taught by Patrick Condreay, PhD, from
GlaxoWellcome R & D. Dr. Condreay has been an instructor for the past 2
years at the ABSA/Eagleson Spring Seminar Series, and is very knowledgeable
and has a terrific way of making the subject very easy to understand. This
will be a very basic course and will provide an understanding of recombinant
DNA methodology.
The entire preconference course listings will be published shortly by ABSA
in the Conference program and registration materials. There will be 10
courses offered over 3 days (10/15, 10/16, and 10/17).
Betsy Gilman
Chair, ABSA Training & Education Committee
Elizabeth A. Gilman, MS, RBP, SM (NRM), CBSP
Laboratory Safety Manager / Biological Safety Officer
Office of Environmental Health & Safety
Boston University Medical Campus
617-638-8842
617-638-8822 fax
egilman@bu.edu
-----Original Message-----
From: Cheri Marcham
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Wednesday, July 28, 1999 3:53 PM
Subject: ATCC recombinant DNA course
>ATCC has an advertisement for a 5 day course entitled, "RECOMBINANT DNA:
>Techniques and Applications" put on by IB3 / George Mason University -
>Manassas, VA on October 4 -8, 1999. Course description says, "A five-day,
>laboratory-intensive, course that covers up-to-date recombinant DNA
>procedures and methodologies both in theory and in practice. This workshop
>provides introductory and intermediate level instruction in
>recombinant DNA laboratory techniques. A basic knowledge of nucleic acids
is
>helpful but not necessary." See
>
>Do any of you know anything about this particular course? Would I be
>completely lost? I have the responsibility of initially reviewing the
>recombinant DNA protocol for safety and compliance issues on our campus
>before going to the IBC for committee approval and am trying desperately to
>find some training to educate me on what it is the researchers are doing so
>I can understand their proposals. This field is so full of acronyms I
>literally have to call the researcher to ask what the lingo in their
>proposal means.
>
>That's why I was hoping the Eagleson Institute would hold their "Biological
>Safety Issues in the Development and Use of Recombinant DNA Gene Delivery
>Technologies" course again, but in the event they do not, I am still
>looking.
>
>Ideas?
>
>Cheri Marcham, CIH, CSP, CHMM
>The University of Oklahoma Health Sciences Center
>cheri-marcham@ouhsc.edu
>(405) 271-3000
>FAX (405) 271-1606
>
=========================================================================
Date: Wed, 28 Jul 1999 16:12:32 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Cheri Marcham
Subject: Re: ATCC recombinant DNA course
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Awesome! Thank you!!
Cheri Marcham
-----Original Message-----
From: Betsy Gilman [mailto:egilman@BU.EDU]
Sent: Wednesday, July 28, 1999 4:13 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: ATCC recombinant DNA course
Dear Group:
In response to Cheri's inquiry about a basic recombinant DNA course, I want
to let everyone know that the ABSA Training & Education Committee will offer
a preconference course on molecular biology and rDNA at the October ABSA
Conference. The course is titled "The Science, Techniques, And Jargon of
Molecular Biology" and will be taught by Patrick Condreay, PhD, from
GlaxoWellcome R & D. Dr. Condreay has been an instructor for the past 2
years at the ABSA/Eagleson Spring Seminar Series, and is very knowledgeable
and has a terrific way of making the subject very easy to understand. This
will be a very basic course and will provide an understanding of recombinant
DNA methodology.
The entire preconference course listings will be published shortly by ABSA
in the Conference program and registration materials. There will be 10
courses offered over 3 days (10/15, 10/16, and 10/17).
Betsy Gilman
Chair, ABSA Training & Education Committee
Elizabeth A. Gilman, MS, RBP, SM (NRM), CBSP
Laboratory Safety Manager / Biological Safety Officer
Office of Environmental Health & Safety
Boston University Medical Campus
617-638-8842
617-638-8822 fax
egilman@bu.edu
-----Original Message-----
From: Cheri Marcham
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Wednesday, July 28, 1999 3:53 PM
Subject: ATCC recombinant DNA course
>ATCC has an advertisement for a 5 day course entitled, "RECOMBINANT DNA:
>Techniques and Applications" put on by IB3 / George Mason University -
>Manassas, VA on October 4 -8, 1999. Course description says, "A five-day,
>laboratory-intensive, course that covers up-to-date recombinant DNA
>procedures and methodologies both in theory and in practice. This workshop
>provides introductory and intermediate level instruction in
>recombinant DNA laboratory techniques. A basic knowledge of nucleic acids
is
>helpful but not necessary." See
>
>Do any of you know anything about this particular course? Would I be
>completely lost? I have the responsibility of initially reviewing the
>recombinant DNA protocol for safety and compliance issues on our campus
>before going to the IBC for committee approval and am trying desperately to
>find some training to educate me on what it is the researchers are doing so
>I can understand their proposals. This field is so full of acronyms I
>literally have to call the researcher to ask what the lingo in their
>proposal means.
>
>That's why I was hoping the Eagleson Institute would hold their "Biological
>Safety Issues in the Development and Use of Recombinant DNA Gene Delivery
>Technologies" course again, but in the event they do not, I am still
>looking.
>
>Ideas?
>
>Cheri Marcham, CIH, CSP, CHMM
>The University of Oklahoma Health Sciences Center
>cheri-marcham@ouhsc.edu
>(405) 271-3000
>FAX (405) 271-1606
>
=========================================================================
Date: Thu, 29 Jul 1999 14:05:13 +0200
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "HAHN, S."
Subject: Info. Request: Xeontransplant Regulation & Clinical Trials .....
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Dear Sir/Mme,
I'm writing a small website about xenotransplant. For web site, I would
like to receive your help and advice. This site is designed to help peoples
find current information about xenotransplant related information on the
web. The site will cover press release, regulation (law), biosafety
concerns, clinical trials, peoples active in this field (including photo
and publication, for this list I need your recommendation), links to
xenotransplant-related web sites of academic and government agencies.
If you would be willing to send me information from your institution, I can
put the information on line and improve the site for others.
It would be very helpful if it comes with permission to use photos, news and
articles for this website and my text.
Thank you very much in advance for your help and advice.
Sincerely,
Hahn, Soo
Montserratstraat 42
1000 Brussel
Belgium
=========================================================================
Date: Thu, 29 Jul 1999 11:46:42 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janet Ives
Organization: Environmental Safety
Subject: Working Alone
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Content-type: text/plain; charset=US-ASCII
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Greetings,
I have been asked by my management to poll you folks about your
policies for working alone in laboratories. I am particularly
interested in how you cope with investigators using BSL-3 labs
alone and after hours. If there is an interest, I will post the results
to the list.
1. Does your institution have a working alone policy?
2. Does your institution conduct research after hours and during
holidays?
3. If you do have a policy, are you willing to share it?
4. How was the policy implemented?
5. Is there any follow through for infractions?
6. Do you have different working alone policies based on the
hazard..chemical vs. biological vs. etc.?
7. Did you have your legal group/risk management review the
situation... policy or no policy?
Thanks very much.
Janet
Janet Ives, Industrial Hygienist
University Risk Management and Environmental Safety
University of Rochester
300 East River Rd. room 23
Rochester, New York 14623
jives@safety.rochester.edu
(716)275-3014 Voice
(716)256-3155 Fax
=========================================================================
Date: Thu, 29 Jul 1999 14:02:30 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Laura Newton
Subject: Re: Any known adverse exposures to sonicated blood
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Greg, the HEPA filter is efficient for particles much smaller than it's
actual pore size, because of the shape of the filter, which is a corrugated
shape. It makes the air pass through a lot of turns and bends, impacting
small particles in the filter medium as they fail to navigate the turns.
The filters are quite thick. It has been well documented and tested that
these filters in a properly certified and operated biological safety cabinet
provide adequate protection for bloodborne pathogens and viral work, and may
be safely exhausted back into the room. Hoods should be certified per NSF
49 at least annually to ensure proper function, and the people using the
hoods should be trained in safe operating practices.
Laura Newton CIH, RBP, CBSP
Industrial Hygiene and Biosafety Consultant
Newton Health and Safety Associates
Flemington, NJ
(908) 284-0258
newtonlb@
-----Original Message-----
From: greg merkle
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Tuesday, July 27, 1999 7:49 PM
Subject: Any known adverse exposures to sonicated blood
>I am looking for information on adverse exposures or health
>risks to the sonication of human blood or components when
>using a class I or II biological safety cabinet that is
>vented back into the lab area. I am in a discussion with a
>faculty member that has concerns over the exhaust HEPA
>filter not being 100% efficent in the removal of
>(potentially) harmful material. The faculty person has
>expressed concern that the laboratory personnel are being
>placed at an unnecessary risk. Is there a need to be
>concerned and demand that a Class I or II BSC be installed
>with a duct to the outside? I am trying to find resources
>to resolve this without it having to go to the full IBC for
>a committee decision if it is not necessary to do so.
>
>Thanks for your help
=========================================================================
Date: Thu, 29 Jul 1999 17:01:54 -0400
Reply-To: btepper@
Sender: A Biosafety Discussion List
From: "Byron S. Tepper"
Organization: @Home Network
Subject: West Coast Biosafety Course
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A three day version of the Control of Biohazards in the Research
Laboratory couse will be presented by Drs. Tepper and Gilpin at the
University of California-San Francisco on September 21-23, 1999 just
prior to the fall BIONET/BSAF Symposium. The course announcement and
registration form is included as an attachment.
If you have a problem opening the attachment, please let me know,
provide you fax number, and I will fax you the announcement.
Byron S. Tepper, PhD, CSP, CBSP
Phone: 410-828-6330
Fax: 410-828-6331
E-mail: btepper@
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--------------392C504D8872426B3B306B60--
=========================================================================
Date: Fri, 30 Jul 1999 08:35:41 +0000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: BRIAN MCSHANE
Subject: BSCs and Use of Carcinogenic Antibiotics
MIME-Version: 1.0
Content-Transfer-Encoding: quoted-printable
Content-Type: text/plain; charset="iso-8859-1"
Does anyone have information concerning the use of biological safety =
cabinets with antibiotic/carcinogens. We are planning on injecting lab =
animals with streptozotocin, cyclosporin and rapamycin all of which are =
considered mutagenic and/or carcinogenic. My initial recommendation is =
the use of a ducted BSC or fume hood for mixing and injecting. Any =
opinions or examples of standard practices? Thanks.
Brian McShane, CIH
Regeneron Pharmaceuticals
brian.mcshane@
=========================================================================
Date: Fri, 30 Jul 1999 08:45:07 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: BSCs and Use of Carcinogenic Antibiotics
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Local (Boston/Cambridge) hospitals stopped using unducted cabinets for the
mixing of these (and other chemicals) as there were adverse health effects
for personnel in the rooms where the unducted BSC were located. Health
effects traced backed to exposure to chemicals being used in the cabinets.
>
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Fri, 30 Jul 1999 10:59:00 -0400
Reply-To: rubockpa@UMDNJ.EDU
Sender: A Biosafety Discussion List
From: Paul Rubock
Organization: eohss-umdnj
Subject: Re: BSCs and Use of Carcinogenic Antibiotics
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
I know that for a number of years, NIOSH among others has recommended using
ducted BSCs for work with chemo. drugs and the comment about tracing health
effects to people in the room where unducted cabinets were used provides
further support.
I do not however have a feel for HOW it happens. These drugs usually have
neglible vapor pressure and even if that were not the case, the in-ward air
flow should take care of that. The HEPA filters will catch any
droplets/aerosols. So the only ROUTE I can envision is:
1-Droplet with aerosol is trapped on HEPA
2-Solute dries up
3-Recrystalization of chemo drug on particles so small they pass thru. the
filter
4-Dispersion into the room, resulting in noted health effects.
PROVIDED THAT THIS ACTIVITY IS DONE WITH A HIGH FREQUENCY (??)
or,
Could it be a question of technique on the part of the preparer??
Am I missing something?
Paul Rubock
Richard Fink wrote:
> Local (Boston/Cambridge) hospitals stopped using unducted cabinets for the
> mixing of these (and other chemicals) as there were adverse health effects
> for personnel in the rooms where the unducted BSC were located. Health
> effects traced backed to exposure to chemicals being used in the cabinets.
>
> >
> Richard Fink, SM(NRM), CBSP
> Assoc. Biosafety Officer
> Mass. Inst. of Tech.
> 617-258-5647
> rfink@mit.edu
=========================================================================
Date: Fri, 30 Jul 1999 10:23:44 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Al Jin
Subject: Re: BSCs and Use of Carcinogenic Antibiotics
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Brian,
The list of drugs mentioned in your QM reminded me of chemotherapy drugs.
As I open my dusty files, it turns out that streptozotocin is listed as an
antibiotic as well as a chemotherapeutic drug.
In section IV(A)2b (aka Guidelines for the handling of CD: Drug Preparation
Area) of an 1986 OSHA document (Instruction PUB 8-1.1: Work Practice
Guidelines for Personnel Dealing with Cytotoxic (Antineoplastic) Drugs), it
states the following:
"A Class II BSC that meets current National Sanitation Foundation Standard
49 must be used. The blower on the vertical airflow hood should be on at
all times, 24 hrs a day, 7 days a week. Venting to the outside is
preferable where feasible, and is required with a Type B BSC. If the hood
has an outside exhaust system, filtered exhaust to the outside should be at
an appropriate level and away from air intake units. BSCs should be
certified by a qualified technician every 6 months or any time the cabinet
is moved".
I hope this information helps. It's alittle dated but I'm sure it has not
changed significantly over the years. In closing, I would suggest staying
away from fume hoods for mixing of materials due to the potential back
draft or turbulence. For injecting animals, I would recommend the use of
portable HEPA filter cage changing stations.
Alfred Jin, BSO, IH, CBSP, M(ASCP), BSS(ASM), CM(ACM),
Hazards Control Department,
Lawrence Livermore National Laboratory,
7000 East Avenue MS-289, Livermore, CA 94550,
Phone:925 423-7385, Fax:423-1086,
Jin2@
>Does anyone have information concerning the use of biological safety
>cabinets with antibiotic/carcinogens. We are planning on injecting lab
>animals with streptozotocin, cyclosporin and rapamycin all of which are
>considered mutagenic and/or carcinogenic. My initial recommendation is
>the use of a ducted BSC or fume hood for mixing and injecting. Any
>opinions or examples of standard practices? Thanks.
>
>Brian McShane, CIH
>Regeneron Pharmaceuticals
>brian.mcshane@
=========================================================================
Date: Fri, 30 Jul 1999 15:39:54 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: BSCs and Use of Carcinogenic Antibiotics
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Brian
In our main inpatient pharmacy we have six 6 foot IIA non-ducted BSCs and one 6
foot IIB ducted BSC that is used for higher hazard gene therapy vectors and
other higher hazard chems, in a separate room (Level II - we call it) to the
side. The advantage of IIA BSCs is that the recirc air into the room helps
clean up the air by repeated filtration and keeps the dust load in the room
down. That's important for products being inoculated into patients. The
disadvantage of unducted IIAs is that they add heat to the room that the ducted
cabinet does not.
These BSCs replaced Class 100 clean benches (blow in your face type) that were
used during the 80s to dispense the antineoplastics. Tests on the urine of the
Pharmacy Techs, conducted by the UT School of Public Health [Dr. Tom Conner - I
think published this] were conducted in ~ the late 80s, b/f my time here.
Metabolites of the antineoplastics rose and fell with the work week/week end.
That's why the clean benches were banned for this work at MD Anderson. We have
not had any problem with antineoplatic exposure or complaints from Pharmacy
techs since the replacement of the clean benches with the unducted IIA BSCs.
However, we do occasionally get complaints from Pharmacy techs about symptoms
that are related to contact exposure, rather than inhalation exposure. And we
have one nurse that is hyperchemical sensitive that has symptoms from
everything. We put her in a PAPR chemical/HEPA respirator. We could never
trace her exposure source down - it could have been the patients' perfume rather
than the antineoplastics as far as we know. but the PAPR solved it.
We are now remoldeling our out-patient pharmacy. We have a combination of 3 IIA
BSCs and 3 IIB BSCs planned for that area. We hope to get the best of both
worlds - cleaner air and lower heat load - with this combination. This will
give us the IIB BSCs to handle any higher hazard antineoplastics or ones that
may have a small degree of volatility with this new set-up. The new room will
also have a HEPA filtered positive pressure air barrier entrance to enhance both
product protection and containment.
BRIAN MCSHANE on 07/30/99 03:35:41 AM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: BSCs and Use of Carcinogenic Antibiotics
Does anyone have information concerning the use of biological safety cabinets
with antibiotic/carcinogens. We are planning on injecting lab animals with
streptozotocin, cyclosporin and rapamycin all of which are considered mutagenic
and/or carcinogenic. My initial recommendation is the use of a ducted BSC or
fume hood for mixing and injecting. Any opinions or examples of standard
practices? Thanks.
Brian McShane, CIH
Regeneron Pharmaceuticals
brian.mcshane@
=========================================================================
Date: Mon, 2 Aug 1999 12:53:24 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Betsy Gilman
Subject: 1999 ABSA Preconference Courses
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Hi Everyone!
I believe that many of you may be interested in the 1999 ABSA =
Preconference Courses. Therefore, I have attached a file with the =
descriptions, dates, times, and instructors for the 10 courses. The =
Training & Education Committee has been working hard to present a mix of =
courses that will appeal to a wide variety of ABSA members. =20
I don't know when ABSA will mail the Conference program and registration =
materials, so hopefully this will be helpful in the meantime. The =
Conference program and registration materials will list the contents of =
the attached file as well as the course fees.
Betsy
Betsy Gilman
Chair, ABSA Training & Education Committee
egilman@bu.edu
------=_NextPart_001_0008_01BEDCE6.02357840
Content-Type: text/html;
charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
Hi Everyone!
I believe that many of you may be interested in the =
1999 ABSA=20
Preconference Courses. Therefore, I have attached a file with the=20
descriptions, dates, times, and instructors for the 10 courses. =
The=20
Training & Education Committee has been working hard to present a =
mix of=20
courses that will appeal to a wide variety of ABSA members. =
I don't know when ABSA will mail the Conference =
program and=20
registration materials, so hopefully this will be helpful in the =
meantime. =20
The Conference program and registration materials will list the contents =
of the=20
attached file as well as the course fees.
Betsy
Betsy Gilman
Chair, ABSA =
Training &=20
Education Committee
egilman@bu.edu
------=_NextPart_001_0008_01BEDCE6.02357840--
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=========================================================================
Date: Wed, 4 Aug 1999 09:35:30 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: alternatives to RAM
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I would like to hear what you have done or experienced at your campuses/in
your business with respect to encouraging researchers and clinical labs to
switch to non-isotope labeling.
Have you done any seminars, encouraged a vendor fair? seen a change?
encountered other hazardous waste issues (re: chemical use increase, waste
increase) seen a change (decrease) in RAM uses?
thanks in advance.
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
=========================================================================
Date: Wed, 4 Aug 1999 13:35:39 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Fran Martin
Subject: Searchable NIOSH Pocket Guide CD-ROM
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
FYI,
The NIOSH Pocket Guide (NPG) and Document Collection is now available in
searchable electronic format on CD-ROM.
It contains detailed chemical hazard information from the National Institute
for Occupational Safety and Health (NIOSH) on more than 670 chemicals, as
well as over 250 MB of supplemental safety information: NIOSH Manual of
Analytical Methods, International Chemical Safety Cards, and various other
publications.
See for details.
Fran Martin
FM Research & Consulting
=========================================================================
Date: Wed, 4 Aug 1999 14:07:27 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Leslie Delpin
Subject: Re: alternatives to RAM
In-Reply-To:
Hi Terry,
In my RSO days, I included information (discussion and product
literature) about non-isotope methods in the Radiation
Safety training sessions offered monthly to isotope users.
Leslie Delpin - Biological Health and Safety Manager
University of Connecticut
Environmental Health and Safety
3102 Horsebarn Hill Rd. Box U-97
Storrs, CT 06269-4097
(860)486-2436
(860)486-1106
lmdelpin@ehs.uconn.edu
=========================================================================
Date: Wed, 4 Aug 1999 17:10:52 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: FW: Level 3 biosafety suit recommendations
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
any ideas anyone?
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
-----Original Message-----
From: Brossia, Christopher [mailto:cbrossia@]
Sent: Wednesday, August 04, 1999 4:52 PM
Subject: Level 3 biosafety suit recommendations
Hi everyone: I would be grateful for any assistance you could provide to us
on this subject. I pulled your names from the ABSA directory. We may need to
conduct some operations involving hantavirus which will require us to
purchase a level 3 biosafety suit. Does anyone have a recommended supplier?
To those of you in Colorado, is there such a vendor on the front range? This
operation will be done in the field (not in a hood) and could involve large
amounts of mice and droppings. We routinely purchase haz mat suits and
related equipment but have never had a need for a biosafety suit. Please
drop me a line if you can. Thanks for your help.
Chris Brossia, MS, CIH
AMGEN
cbrossia@
303-401-1668
303-938-6210(Fax)
=========================================================================
Date: Thu, 5 Aug 1999 07:47:02 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: FW: Level 3 biosafety suit recommendations
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Hi christopher
An appropriate biosafety suite would be like those you may have seen in the
movie "Outbreak". These had a Tyvek (white) zippered disposable jump suit
(available from many suppliers) + a (PAPR) Powered Air Purifying Respirator
(belt battery-pack filtration cartridges) w/ tyvek hood. RACAL Health & Safety
of Frederick Maryland 800 682-9500 makes a Breathe-Easy
system that works well and is light weight. 3M makes a similar one. Get a
PAPR with a HEPA cartridge set. The battery pack needs to be recharged nightly
for 8 hours of work next day - so need the turbo charger unit with it. One
PAPR unit + turbo charger and cartridges ~ $800. A five unit set up ~ $4000.
It charges 5 batteries at a time. Needs daily maintenance & comes w/
instruction book.
Judy Pointer, CBSP, BSO
UTMDACC
"Therese M. Stinnett" on 08/04/99 06:10:52 PM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: FW: Level 3 biosafety suit recommendations
any ideas anyone?
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
-----Original Message-----
From: Brossia, Christopher [mailto:cbrossia@]
Sent: Wednesday, August 04, 1999 4:52 PM
Subject: Level 3 biosafety suit recommendations
Hi everyone: I would be grateful for any assistance you could provide to us
on this subject. I pulled your names from the ABSA directory. We may need to
conduct some operations involving hantavirus which will require us to
purchase a level 3 biosafety suit. Does anyone have a recommended supplier?
To those of you in Colorado, is there such a vendor on the front range? This
operation will be done in the field (not in a hood) and could involve large
amounts of mice and droppings. We routinely purchase haz mat suits and
related equipment but have never had a need for a biosafety suit. Please
drop me a line if you can. Thanks for your help.
Chris Brossia, MS, CIH
AMGEN
cbrossia@
303-401-1668
303-938-6210(Fax)
=========================================================================
Date: Thu, 5 Aug 1999 08:25:46 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Terry Howard
Subject: Re: Level 3 biosafety suit recommendations
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I would need to know the distinction between a "hazmat" suit and a
"biosafety" suit. I would think the criteria for protection from toxic
particulates would be very similar to the criteria for protection from
pathogenic aerosols, but the chemical resistance issue may be unimportant.
A Powered Air Purifying Respirator with a HEPA filter and a sealed suit that
can be decontaminated should be adequate.
-----Original Message-----
From: Therese M. Stinnett [mailto:Therese.Stinnett@UCHSC.EDU]
Sent: Wednesday, August 04, 1999 5:11 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: FW: Level 3 biosafety suit recommendations
any ideas anyone?
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
-----Original Message-----
From: Brossia, Christopher [mailto:cbrossia@]
Sent: Wednesday, August 04, 1999 4:52 PM
Subject: Level 3 biosafety suit recommendations
Hi everyone: I would be grateful for any assistance you could provide to us
on this subject. I pulled your names from the ABSA directory. We may need to
conduct some operations involving hantavirus which will require us to
purchase a level 3 biosafety suit. Does anyone have a recommended supplier?
To those of you in Colorado, is there such a vendor on the front range? This
operation will be done in the field (not in a hood) and could involve large
amounts of mice and droppings. We routinely purchase haz mat suits and
related equipment but have never had a need for a biosafety suit. Please
drop me a line if you can. Thanks for your help.
Chris Brossia, MS, CIH
AMGEN
cbrossia@
303-401-1668
303-938-6210(Fax)
=========================================================================
Date: Thu, 5 Aug 1999 10:13:18 -0500
Reply-To: "mkinsey@"
Sender: A Biosafety Discussion List
From: Melina Kinsey
Organization: MRI
Subject: Re: FW: Level 3 biosafety suit recommendations
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
I agree with Judy's recommendation. Also, if your field work involves cleaning
up the dropping with bleach or other chlorinated compounds in an enclosed space
(attic, room, etc), I would recommend using a dual HEPA/acid gas filter on the
PAPR units. We have used these dual filters with great success.
Good luck
Melina Kinsey
Biosafety Officer
Midwest Research Institute
425 Volker Blvd.
Kansas City, MO 64110
(816) 753-7600 x1424
mkinsey@
-----Original Message-----
From: Judy Pointer [SMTP:jpointer@NOTES.MDACC.TMC.EDU]
Sent: Thursday, August 05, 1999 7:47 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: FW: Level 3 biosafety suit recommendations
Hi christopher
An appropriate biosafety suite would be like those you may have seen in the
movie "Outbreak". These had a Tyvek (white) zippered disposable jump suit
(available from many suppliers) + a (PAPR) Powered Air Purifying Respirator
(belt battery-pack filtration cartridges) w/ tyvek hood. RACAL Health & Safety
of Frederick Maryland 800 682-9500 makes a Breathe-Easy
system that works well and is light weight. 3M makes a similar one. Get a
PAPR with a HEPA cartridge set. The battery pack needs to be recharged nightly
for 8 hours of work next day - so need the turbo charger unit with it. One
PAPR unit + turbo charger and cartridges ~ $800. A five unit set up ~ $4000.
It charges 5 batteries at a time. Needs daily maintenance & comes w/
instruction book.
Judy Pointer, CBSP, BSO
UTMDACC
"Therese M. Stinnett" on 08/04/99 06:10:52 PM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: FW: Level 3 biosafety suit recommendations
any ideas anyone?
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
-----Original Message-----
From: Brossia, Christopher [mailto:cbrossia@]
Sent: Wednesday, August 04, 1999 4:52 PM
Subject: Level 3 biosafety suit recommendations
Hi everyone: I would be grateful for any assistance you could provide to us
on this subject. I pulled your names from the ABSA directory. We may need to
conduct some operations involving hantavirus which will require us to
purchase a level 3 biosafety suit. Does anyone have a recommended supplier?
To those of you in Colorado, is there such a vendor on the front range? This
operation will be done in the field (not in a hood) and could involve large
amounts of mice and droppings. We routinely purchase haz mat suits and
related equipment but have never had a need for a biosafety suit. Please
drop me a line if you can. Thanks for your help.
Chris Brossia, MS, CIH
AMGEN
cbrossia@
303-401-1668
303-938-6210(Fax)
=========================================================================
Date: Thu, 5 Aug 1999 13:00:32 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Kenneth Hallatt
Subject: Decontamination and Detergent
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: quoted-printable
I have some folks who would like to use a product called RBS for decontamin=
ation of lab equipment because it can also strip away protein residues. I =
asked if it was Tuberculocidal and the product does not have that claim. =
While the product info for the RBS states it can "decontaminate", further =
searching showed that it has not been tested against Hep B. End of the =
line for RBS as a decontamination agent in my labs.
I was curious if anyone out there was familiar with a decontamination =
agent that might fit the bill for these folks. Please let me know.
Thanks
=========================================================================
Date: Thu, 5 Aug 1999 12:17:54 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: FW: Level 3 biosafety suit recommendations
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Melissa's got a good point. We've used the PAPRs w/ the HEPA/acid gas
cartridges for confined space, surge tank chlorination b/f. The workers really
liked them. But you do get break through pretty quick on the chlorine, so be
sure and train them, warn them, and have extra acid gas cartridges available in
the field.
=========================================================================
Date: Thu, 5 Aug 1999 13:25:01 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Betty Kupskay
Subject: Re: Decontamination and Detergent
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
The TB lab at the Canadian Science Centre uses a phenolic detergent called S7
which is "an economical, liquid phenolic based disinfectant/detergent" and "has
been tested and found effective against E. coli and S. aureus at 7.75 ml/l and
against P. aeruginosa and M. smegmatis (for tuberculocidal activity) at 12.5
ml/l".
It can be obtained from Diversey Inc., Mississauga, ON 905-822-3511. If you need
product info sheet and MSDS, please let me know and I'll FAX it to you.
Ciao!
Betty Kupskay
Biosafety Specialist/Health Canada
Canadian Science Centre for Human and Animal Health
1015 Arlington St., Suite A1010
Winnipeg, MB R3E 3P6
Ph: 204-789-2065
Fax: 204-789-2069
EMail: betty_kupskay@hc-sc.gc.ca
Kenneth Hallatt on 99/08/05 12:00:32 PM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Betty Kupskay)
Subject: Decontamination and Detergent
I have some folks who would like to use a product called RBS for decontamination
of lab equipment because it can also strip away protein residues. I asked if it
was Tuberculocidal and the product does not have that claim. While the product
info for the RBS states it can "decontaminate", further searching showed that it
has not been tested against Hep B. End of the line for RBS as a decontamination
agent in my labs.
I was curious if anyone out there was familiar with a decontamination agent that
might fit the bill for these folks. Please let me know.
Thanks
=========================================================================
Date: Mon, 9 Aug 1999 08:07:26 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Betsy Gilman
Subject: NRM Exam Review Course
MIME-Version: 1.0
Content-Type: multipart/alternative;
boundary="----=_NextPart_000_000D_01BEE23E.37F7F7C0"
This is a multi-part message in MIME format.
------=_NextPart_000_000D_01BEE23E.37F7F7C0
Content-Type: text/plain;
charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
Recently I posted course descriptions for the 10 ABSA preconference =
courses to be offered in St. Louis in October. The instructors for the =
2.5 day NRM Exam Review Course would like to get an idea of how many =
people are planning to sign up for this course, and what their =
background is. This information will assist them in planning the =
course. Please note that this is not an introductory course, and that =
there is a prerequisite for general biosafety knowledge. The course =
dates are 10/15-10/17.
If you are planning to register (as soon as ABSA mails out the =
registration info.) for the NRM Exam Review Course, please let me know. =
Also, let me know how many years of experience in the field of biosafety =
you have. Do not reply to the list, simply email me at egilman@bu.edu. =
Many thanks!
Betsy Gilman
Chair, ABSA Training & Education Committee
Elizabeth A. Gilman, MS, RBP, SM (NRM), CBSP
Laboratory Safety Manager / Biological Safety Officer
Office of Environmental Health & Safety
Boston University Medical Campus
617-638-8842
617-638-8822 fax
egilman@bu.edu
------=_NextPart_000_000D_01BEE23E.37F7F7C0
Content-Type: text/html;
charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
Recently I posted course descriptions for the 10 =
ABSA=20
preconference courses to be offered in St. Louis in October. The=20
instructors for the 2.5 day NRM Exam Review Course would like to get an =
idea of=20
how many people are planning to sign up for this course, and what their=20
background is. This information will assist them in planning the=20
course. Please note that this is not an introductory course, and =
that=20
there is a prerequisite for general biosafety knowledge. The =
course dates=20
are 10/15-10/17.
If you are planning to register (as soon as ABSA =
mails out the=20
registration info.) for the NRM Exam Review Course, please let me =
know. =20
Also, let me know how many years of experience in the field of biosafety =
you=20
have. Do not reply to the list, simply email me at egilman@bu.edu. Many =
thanks!
Betsy Gilman
Chair, ABSA Training & Education =
Committee
Elizabeth A. Gilman, MS, RBP, SM =
(NRM),=20
CBSPLaboratory Safety Manager / Biological Safety OfficerOffice =
of=20
Environmental Health & SafetyBoston University Medical=20
Campus617-638-8842617-638-8822 faxegilman@bu.edu
------=_NextPart_000_000D_01BEE23E.37F7F7C0--
=========================================================================
Date: Mon, 9 Aug 1999 11:16:53 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Elizabeth Smith
Subject: Re: Decontamination and Detergent
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Our facility uses good old fashioned bleach for most routine
disinfectant needs, supplemented occasionally by commercially
available products, including SporeKlenz, LpHse and 1 Stroke
Environ.
Due to the organisms we use, occasionally we find that
commercially available disinfectants DON'T have product
verification available for us. The 'hey, can you tell me
whether your stuff kills my bugs?' question.
For some really stubborn microorganisms, we've had to do
in-house studies to determine the efficacy of various products.
Not a real mental challenge, but it does take some time to do.
Elizabeth
===
Elizabeth Smith
Environmental, Health & Safety
BioPort Corporation
3500 N. Martin L. King Blvd.
Lansing, MI 48906
517-327-1590
_____________________________________________________________
Do You Yahoo!?
Bid and sell for free at
=========================================================================
Date: Tue, 10 Aug 1999 08:44:20 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: training for environmental services & facilities personnel
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
It is time to review our training for new employees in these two areas, as
well as refresher training for the "seasoned vets"
If you are doing this training for this population, I would appreciate some
feedback. I think we can give initial training over a 2 hour period that
would cover:
Introduction to the Laboratory Environment (we only have the
research & a couple of clinical labs; lots of administrative & classroom
areas. Hospital is separate) 15 minutes
BBP/OPIM Exposure, to include Sharps containers, transportation of
Infectious Waste containers, research animal exposures, about 45 minutes
Personal Protective equipment: gloves, aprons, splash hazards and
eye/face protection, 15 minutes
Radioactive Materials safety training, about 45 minutes
Q&A time
We have interpreters available to assist. We have about 60 personnel to
train for housekeeping, in refresher training, and about 2 new hires per
month. There is less turnover in the maintenance ranks, but still around 2
new hires per month. We intend to rely very heavily on graphics
presentations and handouts, with some laminated handouts to be placed in
their "janitors closets" or lockers, or where deemed appropriate.
So, what am I forgetting?
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
=========================================================================
Date: Tue, 10 Aug 1999 14:07:21 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: training for environmental services & facilities personnel
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
BBP requires an exposure control plan. New personnel must become familiar
with the requirements.
The document must be reviewed and updated at least annualy.
Any changes must be presented during retraining. We believe that the
workers should read the ECP again.
Bob
>It is time to review our training for new employees in these two areas, as
>well as refresher training for the "seasoned vets"
>
>If you are doing this training for this population, I would appreciate some
>feedback. I think we can give initial training over a 2 hour period that
>would cover:
> Introduction to the Laboratory Environment (we only have the
>research & a couple of clinical labs; lots of administrative & classroom
>areas. Hospital is separate) 15 minutes
> BBP/OPIM Exposure, to include Sharps containers, transportation of
>Infectious Waste containers, research animal exposures, about 45 minutes
> Personal Protective equipment: gloves, aprons, splash hazards and
>eye/face protection, 15 minutes
> Radioactive Materials safety training, about 45 minutes
> Q&A time
>
>We have interpreters available to assist. We have about 60 personnel to
>train for housekeeping, in refresher training, and about 2 new hires per
>month. There is less turnover in the maintenance ranks, but still around 2
>new hires per month. We intend to rely very heavily on graphics
>presentations and handouts, with some laminated handouts to be placed in
>their "janitors closets" or lockers, or where deemed appropriate.
>
>So, what am I forgetting?
>
>Therese M. Stinnett
>Biosafety Officer
>Health and Safety Division
>UCHSC, Mailstop C275
>Phone: 303-315-6754
>Pager: 303-266-5402
>Fax: 303-315-8026
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Wed, 11 Aug 1999 10:06:24 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Hofherr, Leslie"
Subject: Shipping infectious substance to Japan
MIME-Version: 1.0
Content-Type: text/plain
A researcher at UCLA would like to ship a frozen vrial prep. to Japan. He
would like it to remain frozen during shipment. He contact Fedx and they
told him it would take them a week to get the sample to the researcher in
Japan.
Please let me know of other couriers that may be able to get the frozen
viral prep. to the researcher in Japan in a day or two. I can be reached at
the e-mail address or phone number below.
Thank You,
Leslie Hofherr
UCLA, EH&S
(310)206-3929
leslie@admin.ucla.edu
=========================================================================
Date: Wed, 11 Aug 1999 11:28:24 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: Re: Shipping infectious substance to Japan
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I used to ship to Japan on a regular basis and I believe the name of the
shipping firm was Kintetsu
phone: 303-288-3228 in the Denver metro area; I imagine they have offices
elsewhere
as I recall (and this was several years ago) because of the flight time it
will be greater than 24 hours in transit; I don't recall the rules on dry
ice; we may have lyophilized the materials we shipped
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
=========================================================================
Date: Wed, 11 Aug 1999 13:27:44 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Re: Shipping infectious substance to Japan
MIME-Version: 1.0
Content-Type: text/plain
World Courier 1.800.221.6600
> ----------
> From: Hofherr, Leslie[SMTP:Leslie@ADMIN.UCLA.EDU]
> Sent: Wednesday, August 11, 1999 1:06 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Shipping infectious substance to Japan
>
> A researcher at UCLA would like to ship a frozen vrial prep. to Japan. He
> would like it to remain frozen during shipment. He contact Fedx and they
> told him it would take them a week to get the sample to the researcher in
> Japan.
>
> Please let me know of other couriers that may be able to get the frozen
> viral prep. to the researcher in Japan in a day or two. I can be reached
> at
> the e-mail address or phone number below.
>
> Thank You,
>
> Leslie Hofherr
> UCLA, EH&S
> (310)206-3929
> leslie@admin.ucla.edu
>
=========================================================================
Date: Wed, 11 Aug 1999 16:58:04 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: FW: FYI: BIOSAFTY discussion on shipping infectious substances to
Japan
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
I forwarded the question about shipping to Japan to a colleague, and am =
now
forwarding his response to the list.
Julie A. Johnson, Ph.D.
Biosafety Officer
Environmental Health and Safety
Iowa State University
Ames, IA 50011
e-mail: jajohns@iastate.edu
phone: 515-294-7657
fax: 515-294-9357
web site:
> Leslie Hofherr,
>=20
> I think that Fed Ex was hedging a little bit, but I'm fairly certain =
it
> would take more than two days (three days at the least). I would be
> surprised to find a carrier that could deliver hazardous materials =
through
> customs in one to two days and am sure that the cost would be high. =
In
> fact, Fed Ex will not guarantee delivery times for hazardous =
materials
> shipped out of the country. As a result, I would recommend that
> sufficient dry ice is used to maintain the desired temperature for a =
week
> or find another way (i.e., lypohilize). Otherwise you would be =
wasting
> time and money. A good rule of thumb on quantity of dry ice to use =
is: 5
> lbs for the 1st 24 hours and 3 lbs for each additional 24 hours when
> packed in a 1 =BD inch thick styrofoam box. =20
>=20
> In addition, it is important to determine if permits are required for
> entry into a country, obtain those necessary and attach a copy to the
> shipping documents. If this is not done the package will sit in =
customs
> of the receiving country or will be refused all together. Also, =
check
> with the carrier to ensure that they have door to door delivery in =
Japan.
> In some cases (depending on the country and the carrier) the carrier =
is
> only able to delivery to the airport, then the receiver would be
> responsible for picking it up at the airport. If this is the case =
ensure
> that the contact in Japan is informed and has copies of all permits
> required.
>=20
> I hope this is helpful. If you have additional questions feel free =
to
> contact me directly.
>=20
>=20
> Richard McColley, Associate Industrial Hygienist
> Iowa State University
> Environmental Health & Safety
> 118 Agronomy Lab
> Ames, IA 50011
> phone: 515-294-9561
> fax: 515-294-9357
> E-mail: remccol@iastate.edu
>
>=20
>=20
> -----Original Message-----
> From: Johnson, Julie A.=20
> Sent: Wednesday, August 11, 1999 1:11 PM
> To: McColley, Richard E.
> Subject: FYI: BIOSAFTY discussion on shipping infectious
> substances to Japan=20
>=20
> > Message: Re: Shipping infectious substance to Japan >> Shipping infectious substance to Japan >>=20
>=20
> Julie A. Johnson, Ph.D.
> Biosafety Officer
> Environmental Health and Safety
> Iowa State University
> Ames, IA 50011
> e-mail: jajohns@iastate.edu
> phone: 515-294-7657
> fax: 515-294-9357
> web site:
>=20
=========================================================================
Date: Thu, 12 Aug 1999 08:20:30 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Replicative deficient Adenovirus Serotype 5
MIME-Version: 1.0
Content-Type: text/plain
Do you recommend handling replication deficient adenovirus serotype 5 at a
BL 1 or a BL 2? NIH rDNA Guidelines classified human adenoviruses as a RG 2
which are associated with human disease which is rarely serious and for
which preventive or therapeutic interventions are often available.
I was going to recommend BL 2 practices, procedures and containment. Can
anyone provide any insight?
Thank you.
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Thu, 12 Aug 1999 14:28:34 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: Shipping infectious substance to Japan
MIME-Version: 1.0
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boundary="----=_NextPart_000_005C_01BEE4CE.F59AA060"
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Leslie:
Samples can be shipped frozen in "dry shippers" or "vapor shippers", which
are a type of Dewar in which liquid N2 is absorbed into a material. They
are specifically designed for transportation of biological materials at
cryogenic temperatures. My understanding is that they will hold temp for a
week. Also, when properly prepared (PI 202) are not subject to the
requirements of Danger Goods Regs, as long as no free liq N2 is present
A good source for shipping materials for cryogenics is MVE, Inc/Caire, Inc;
Burnsville, MN, 612-882-5000.
-----Original Message-----
From: Hofherr, Leslie
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Wednesday, August 11, 1999 1:14 PM
Subject: Shipping infectious substance to Japan
>A researcher at UCLA would like to ship a frozen vrial prep. to Japan. He
>would like it to remain frozen during shipment. He contact Fedx and they
>told him it would take them a week to get the sample to the researcher in
>Japan.
>
>Please let me know of other couriers that may be able to get the frozen
>viral prep. to the researcher in Japan in a day or two. I can be reached at
>the e-mail address or phone number below.
>
>Thank You,
>
>Leslie Hofherr
>UCLA, EH&S
>(310)206-3929
>leslie@admin.ucla.edu
>
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=========================================================================
Date: Thu, 12 Aug 1999 14:58:57 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: Replicative deficient Adenovirus Serotype 5
MIME-Version: 1.0
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boundary="----=_NextPart_000_006D_01BEE4D3.33C910C0"
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Bliss:
We have a protocol for repl. defective adenovirus, and I have been looking
into this a great deal. Virologists both here and at Princeton have told me
that one can never assume a repl defective strain will be completely
defective. Some small percentage will retain the wild type characteristics.
The numbers can be magnified through serial passage or by large scale
operations.
Although adenovirus is a relatively safe virus, there are those who can have
serious consequences, particularly to type 5. Immunocompromised people can
have fatal infections.
The bottom for us is BL2.
Janice Flesher, MS
Principle Industrial Hygienist/Biosafety Officer
EOHSS - University Medical Dental School of NJ
97 Paterson St. #227
New Brunswick, NJ, 08901
(732) 235-8497 phone
(732) 235-8499 fax
fleshejk@umdnj.edu
-----Original Message-----
From: Schlank Bliss BM
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Thursday, August 12, 1999 8:23 AM
Subject: Replicative deficient Adenovirus Serotype 5
>Do you recommend handling replication deficient adenovirus serotype 5 at a
>BL 1 or a BL 2? NIH rDNA Guidelines classified human adenoviruses as a RG
2
>which are associated with human disease which is rarely serious and for
>which preventive or therapeutic interventions are often available.
>
>I was going to recommend BL 2 practices, procedures and containment. Can
>anyone provide any insight?
>
>Thank you.
>
>Bliss M. Schlank
>Biosafety Specialist
>AstraZeneca
>1800 Concord Pike
>Wilmington DE 19850-5437
>302.886.2185 Fax: 302.886.2909
>bliss.schlank@phwilm.
>
>
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TEL;HOME;VOICE:609-620-1206
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=========================================================================
Date: Thu, 12 Aug 1999 15:27:57 -0400
Reply-To: Philippe-Alexandre Gilbert
Sender: A Biosafety Discussion List
From: Philippe-Alexandre Gilbert
Organization: =?iso-8859-1?Q?Universit=E9_Laval?=
Subject: Re: Replicative deficient Adenovirus Serotype 5
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
> into this a great deal. Virologists both here and at Princeton have to=
ld
me
> that one can never assume a repl defective strain will be completely
> defective. Some small percentage will retain the wild type
characteristics.
> The numbers can be magnified through serial passage or by large scale
> operations.
The reference for this fact is in :
Lochmuller H, et al.
Emergence of early region 1-containing replication-competent adenovirus i=
n
stocks of replication-defective adenovirus recombinants (delta E1 + delta
E3) during multiple passages in 293 cells.
Hum Gene Ther. 1994 Dec;5(12):1485-91.
This discussion brings another question, maybe answered previously.
Since 293 cells (or any cells complementing the repl defective adenovirus=
),
contains the sequence E1 (and sometimes E2) of the adenoviral genome,
should it be considered BL2 ? Even if it's just (strictly) for the
maintenance of the cells ?
PAG
>>>>>>>>>>>>>>>>>>>>>> by a reputable medical waste hauler for further processing by
> autoclave or incineration. These labs generate wastes that result
> from BSL-1 and -2 activities. A proposal has been presented to
> eliminate on-site treatment, and to rely only upon the off-site
> decontamination of this waste. Have we been performing an 'overkill'
> procedure, or are there legitimate reasons for maintaining the on-site
We autoclaved the infectious waste ( not sharp containers) and then treat
it as regular waste. Note that NY may have different regulations than MN.
Your present procedures seem like overkill to me.
>
>
> Paul Zel, RBP, CHMM
> Director, Environmental Health & Safety
> Memorial Sloan-Kettering Cancer Center
> New York, N.Y.
> zelp@
Jim Lauer
University of Minnesota
Environmental Health & Safety
W-158
626-5621
lauer001@maroon.tc.umn.edu
=========================================================================
Date: Mon, 16 Aug 1999 07:39:03 EDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Re: biohazard waste processing
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Paul:
Primarily because of your location, you are in an interesting position.
Clinical microbiology labs in NYC must autoclave all cultures and stocks
(city ordinance). However, I'm not sure this applies to research facilities.
Technically, as long as your waste is treated, is would be considered solid
waste. However, if it stays in NY, your hauler and disposal site must have
appropriate paper work indicating waste has been properly treated (as well as
the landfill/treatment facility is permitted to take treated medical waste).
If your waste leaves the state, generator beware! Your waste takes on a
different perspective
In a message dated 8/13/99 6:38:39 PM,
Zel_Paul/mskcc_SAFETY@MSKMAIL. writes:
=========================================================================
Date: Mon, 16 Aug 1999 07:45:53 EDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Re: biohazard waste processing
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Let's try again!
Paul:
Primarily because of your location, you are in an interesting position.
Clinical microbiology labs in NYC must autoclave all cultures and stocks
(city ordinance). However, I'm not sure this applies to research facilities.
Technically, as long as your waste is treated, is would be considered solid
waste. However, if it stays in NY, your hauler and disposal site must have
appropriate paper work indicating waste has been properly treated (as well as
the landfill/treatment facility is permitted to take treated medical waste).
If your waste leaves the state, generator beware! Your waste takes on a
different perspective depending on where it goes for disposal.
Autoclaving of cultures and stocks regardless of the type is good laboratory
practice. Interestingly, while many hospitals in the US have gotten away form
this, it is still a common practice in other parts of the world. Just
returned from Hong Kong where all micro labs in public hospitals treat
cultures and stocks onsite.
Ed Krisiunas, MT(ASCP), CIC, MPH
Director
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
860-306-6658(mobile)
=========================================================================
Date: Mon, 16 Aug 1999 09:31:52 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: biohazard waste processing
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Paul,
Our procedures are very similar to yours. In, Ohio, Either treatment at
the site or treatment at the TSDF is acceptable. We treat on site to make
the waste easier to manage. We do not treat to meet state regulations.
The TSDF treats to meet state regulations.
I am not up on any NY laws that you may have to comply with. So I cannot
advise you there.
Our feeling is that by reducing the threat presented by the hazard at the
site, we reduce the risk of an accident.
Bob
> Currently, our research laboratory biohazard waste (not including
> sharps containers) is treated by steam sterilization prior to
> packaging as regulated medical waste. The waste is then transported
> by a reputable medical waste hauler for further processing by
> autoclave or incineration. These labs generate wastes that result
> from BSL-1 and -2 activities. A proposal has been presented to
> eliminate on-site treatment, and to rely only upon the off-site
> decontamination of this waste. Have we been performing an 'overkill'
> procedure, or are there legitimate reasons for maintaining the on-site
> decontamination process? Your comments or experiences would be
> appreciated. Thanks.
>
>
> Paul Zel, RBP, CHMM
> Director, Environmental Health & Safety
> Memorial Sloan-Kettering Cancer Center
> New York, N.Y.
> zelp@
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Mon, 16 Aug 1999 11:03:58 -0400
Reply-To: jkeene@
Sender: A Biosafety Discussion List
From: "John H. Keene"
Subject: Re: biohazard waste processing
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Paul, People from all over the country can give you advice on what to do
with your waste from your research labs, but you have to comply with the NY
state and local regulations regarding treatment, transport and disposal of
that waste. It is always appropriate to treat, by autoclaving or chemical
decontamination, prior to removal from the lab in order to minimize the
potential for occupational exposure to that waste. However, treatment on
site is not always accepted by the regulators who are concerned,
realistically or politically, with potential environmental problems. Bottom
Line, for protection of your own housekeeping personnel, continue to decon
the waste, and, in addition, follow the current state and local regulations
regarding the treatment, transport and disposal of "regulated medical
waste".
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Robert N. Latsch
Sent: Monday, August 16, 1999 10:32 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: biohazard waste processing
Paul,
Our procedures are very similar to yours. In, Ohio, Either treatment at
the site or treatment at the TSDF is acceptable. We treat on site to make
the waste easier to manage. We do not treat to meet state regulations.
The TSDF treats to meet state regulations.
I am not up on any NY laws that you may have to comply with. So I cannot
advise you there.
Our feeling is that by reducing the threat presented by the hazard at the
site, we reduce the risk of an accident.
Bob
> Currently, our research laboratory biohazard waste (not including
> sharps containers) is treated by steam sterilization prior to
> packaging as regulated medical waste. The waste is then transported
> by a reputable medical waste hauler for further processing by
> autoclave or incineration. These labs generate wastes that result
> from BSL-1 and -2 activities. A proposal has been presented to
> eliminate on-site treatment, and to rely only upon the off-site
> decontamination of this waste. Have we been performing an 'overkill'
> procedure, or are there legitimate reasons for maintaining the on-site
> decontamination process? Your comments or experiences would be
> appreciated. Thanks.
>
>
> Paul Zel, RBP, CHMM
> Director, Environmental Health & Safety
> Memorial Sloan-Kettering Cancer Center
> New York, N.Y.
> zelp@
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Mon, 16 Aug 1999 09:18:18 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Al Jin
Subject: Re: biohazard waste processing
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Paul
We at LLNL also follow this practice of first autoclaving the sharps
containers prior to sending them to a incinerator for total destruction.
The sharps waste is generated at different sites within a building and is
brought to a single location for disposal, the steam sterilization is
performed by our glassware/ storeroom employees. The onsite sterilization
is ensures the following:
1. the initial destruction of any potential pathogens present converts the
infectious sharps waste to solid sharp waste and can be handled as such.
2. generators ownership to the waste generator.
3. accoutability on the amounts generated as required by State Law.
4. closure on the "cradle to grave" issue.
Being a DOE facility, we prefer to take the additional steps to ensure that
all has been perform to ensure the general public and community that our
facility is operating as safe as possible. It's peace of mind, the cost is
minimual to ensure that we are not on the 6 O Clock news.
In closing, whether or not you continue it is up to your management. In my
opinon, the cost saving is minimal.
Alfred Jin, BSO, IH, MS, CBSP, M(ASCP), BSS(ASM), CM(ACM),
Hazards Control Department,
Lawrence Livermore National Laboratory,
7000 East Avenue MS-289,
Livermore, CA 94550,
Phone:925 423-7385, Fax:423-1086,
Jin2@
> Currently, our research laboratory biohazard waste (not including
> sharps containers) is treated by steam sterilization prior to
> packaging as regulated medical waste. The waste is then transported
> by a reputable medical waste hauler for further processing by
> autoclave or incineration. These labs generate waste that result from
> BSL-1 and -2 activities. A proposal has been presented to eliminate
> on-site treatment, and to rely only on the off-site decontamination of
> this waste. Have we been performing an 'overkill' procedure, or are
> there legitimate reasons for maintaining the on-site decontamination
> process? Your comments or experiences would be appreciated. Thanks.
>
>
> Paul Zel, RBP, CHMM
> Director, Environmental Health & Safety
> Memorial Sloan-Kettering Cancer Center
> New York, N.Y.
> zelp@
=========================================================================
Date: Mon, 16 Aug 1999 13:23:20 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Paul Jennette
Subject: Re: biohazard waste processing
In-Reply-To:
Mime-Version: 1.0
Content-Type: multipart/alternative;
boundary="=====================_18797348==_.ALT"
--=====================_18797348==_.ALT
Content-Type: text/plain; charset="us-ascii"
From a regulatory standpoint, RMW only has to be treated once in New York
State, and the definition of "treated" and associated regulatory requirements
depend on who is doing the treating:
- The RMW hauler you're using should be taking the waste to commercial
facilities that are permitted under the applicable NYS environmental
regulations (6NYCRR Part 360-17). At these facilities, the RMW is both
"treated" (i.e., rendered noninfectious) and destroyed (i.e., made
unrecognizable). From this standpoint, your onsite steam sterilization is
overkill.
- You might, however, want to autoclave your RMW anyway for worker safety (or
perception) reasons, or if local regs require it. If so, the question of
whether you could simply treat RMW (non-sharps) onsite and dispose of it with
ordinary solid waste arises. The answer is maybe, if you meet the NYS DOH and
DEC's requirements for treatment type, approvals/permits, testing,
documentation, etc. Guidance can be found in DOH's "Managing Regulated Medical
Waste" (1995) and DEC's "Guidance for RMW Treatment, Storage, Containment,
Transport, and Disposal" (1996). Both are available from DEC's solid waste
main office in Albany at (518) 457-2051. Good Luck.
At 06:31 PM 8/13/99 -0400, you wrote:
> Currently, our research laboratory biohazard waste (not including
> sharps containers) is treated by steam sterilization prior to
> packaging as regulated medical waste. The waste is then transported
> by a reputable medical waste hauler for further processing by
> autoclave or incineration. These labs generate wastes that result
> from BSL-1 and -2 activities. A proposal has been presented to
> eliminate on-site treatment, and to rely only upon the off-site
> decontamination of this waste. Have we been performing an 'overkill'
> procedure, or are there legitimate reasons for maintaining the on-site
> decontamination process? Your comments or experiences would be
> appreciated. Thanks.
>
>
> Paul Zel, RBP, CHMM
> Director, Environmental Health & Safety
> Memorial Sloan-Kettering Cancer Center
> New York, N.Y.
> zelp@
J. Paul Jennette, P.E.
Biosafety Engineer
Cornell University
College of Veterinary Medicine
Biosafety Program
S3-010 Schurman Hall, Box 38 (607) 253-4227
Ithaca, New York 14853-6401 fax -3723
--=====================_18797348==_.ALT
Content-Type: text/html; charset="us-ascii"
From a regulatory standpoint, RMW only has to be treated once
in New York State, and the definition of "treated" and
associated regulatory requirements depend on who is doing the treating:
- The RMW hauler you're using should be taking the waste to
commercial facilities that are permitted under the applicable NYS
environmental regulations (6NYCRR Part 360-17). At these
facilities, the RMW is both "treated" (i.e., rendered
noninfectious) and destroyed (i.e., made unrecognizable). From this
standpoint, your onsite steam sterilization is overkill.
- You might, however, want to autoclave your RMW anyway for worker
safety (or perception) reasons, or if local regs require it. If so,
the question of whether you could simply treat RMW (non-sharps) onsite
and dispose of it with ordinary solid waste arises. The answer is
maybe, if you meet the NYS DOH and DEC's requirements for treatment type,
approvals/permits, testing, documentation, etc. Guidance can be
found in DOH's "Managing Regulated Medical Waste" (1995) and
DEC's "Guidance for RMW Treatment, Storage, Containment, Transport,
and Disposal" (1996). Both are available from DEC's solid
waste main office in Albany at (518) 457-2051. Good Luck.
At 06:31 PM 8/13/99 -0400, you wrote:
> Currently, our research laboratory
biohazard waste (not including
> sharps containers) is treated by steam
sterilization prior to
> packaging as regulated medical
waste. The waste is then transported
> by a reputable medical waste hauler for
further processing by
> autoclave or incineration. These
labs generate wastes that result
> from BSL-1 and -2 activities. A
proposal has been presented to
> eliminate on-site treatment, and to
rely only upon the off-site
> decontamination of this waste.
Have we been performing an 'overkill'
> procedure, or are there legitimate
reasons for maintaining the on-site
> decontamination process? Your
comments or experiences would be
> appreciated. Thanks.
>
>
> Paul Zel, RBP, CHMM
> Director, Environmental Health &
Safety
> Memorial Sloan-Kettering Cancer
Center
> New York, N.Y.
> zelp@
J. Paul Jennette, P.E.
Biosafety Engineer
Cornell University
College of Veterinary Medicine
Biosafety Program
S3-010 Schurman Hall, Box 38 (607)
253-4227
Ithaca, New York
14853-6401 fax
-3723
--=====================_18797348==_.ALT--
=========================================================================
Date: Tue, 17 Aug 1999 20:06:17 EDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: Ratio of Safety Personnel
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
What is the average ratio of employees to HSE staff in
non-academic institutions? ... Jim
*****************************************************
James A. Kaufman, Director
The Laboratory Safety Workshop
Safety in Science Education
192 Worcester Road, Natick, MA 01760
508-647-1900 Fax: 508-647-0062 Cell: 508-574-6264
Email: labsafe@ Web Site:
******************************************************
=========================================================================
Date: Wed, 18 Aug 1999 07:58:32 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Aerosol Monitor
MIME-Version: 1.0
Content-Type: text/plain
Has anyone ever used an aerosol monitor - I have found one and was
interested in your feedback.
This particular monitor is made by PPM Enterprises and the Model is 1005.
Thanks for your input.
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Wed, 18 Aug 1999 13:46:08 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ninni Jacob
Subject: Disposal of Refrigerators used for cadavers
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Our Anatomy department is replacing old refrigerators and freezers that
were used to store cadavers and animal remains for many years.
The company who is going to do the removal wants to know:
1. Are there any special precautions they have to take.
2. Can they dispose of these the normal way (like other appliances)?
3. Is it only a chemical hazard or is it biological too at this point?
4. Does it have to be decontaminated in some way?
I would like to hear from those of you who have had experience in this area.
Thanks for your input.
Sincerely:
Ninni Jacob, CHP
Radiation and Biological Safety Officer
Office of Risk Management
Brown University - Box 1914
164 Angell Street
Providence, RI 02912
Tel:401 863 1738
Fax:401 863 7676
email: Ninni_Jacob@brown.edu
=========================================================================
Date: Wed, 18 Aug 1999 14:59:46 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Disposal of Refrigerators used for cadavers
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Since it is difficult to know how well all of the remains were preserved,
i.e. whether everything was properly soaked in formaldehyde, I would be
cautious and decon the refrigerators and freezer. After the decon, then
they could be disposed of like any other appliance. It is unlikely that
the motor area would have any contamination.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Wed, 18 Aug 1999 15:46:34 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Job Announcement- Biological Safety
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
>
>Environmental Health & Safety Specialist
>
>Tulane University Medical Center, Department of Environmental Health &
>Safety is seeking a qualified candidate to coordinate
>the Infectious Disease and Biological Safety Program for Tulane University.
>
>Individual is responsible for all occupational and environmental health and
>safety compliance with federal, state, and local
>regulations as they relate to the program (OSHA Bloodborne and TB
>Prevention Program and Recombinant DNA and Gene
>Therapy Research). Will participate in EHS Emergency On-Call Program for
>safety and health issues at Tulane.
>
>Bachelor's Degree in Biological Science required, (Microbiology,
>Environmental or Occupational Science preferred). Minimum
>of 3 years combined clinical/educational experience with emphasis on
>infection control/infectious diseases desired. Safety
>experience in laboratory safety, industrial hygiene,
>occupational/environmental protection is a plus. Must possess excellent
>written and verbal communication skills, computer skills required. Strong
>background in microbiology is essential. Professional
>Bio-safety certification or eligibility is desirable.
>
>Qualified candidates should send resume along with cover letter and 3
>references on or before September 30, 1999 to:
>
>Tulane University Medical Center
>Human Resources
>1430 Tulane Avenue EP-13
>New Orleans, LA 70112
>
>AA/EOE
>
>
>
>
>
>
>
>
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
=========================================================================
Date: Wed, 18 Aug 1999 16:08:31 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Stetz, Sharon"
Subject: BSC's in Research Hospitals
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I am fairly new to the biosafety-community, have learned quite alot already
since subscribing to this list at the end of June (1999), and have a
significant chemical safety background. With that out of the way, I have
inherited the task of assessing our various Pathology laboratories for
compliance with the accepted biosafety standards of practice. I work in a
large, urban, research hospital that has a significant Infectious Diseases
Department and treatment unit for both adults and pediatrics. I have been
reading the BMBL but still have a few basic questions and would really
appreciate some input.
1. Wouldn't Pathology laboratories that take specimen samples from
limbs and various organs to perform the requested diagnostics need to use at
least some type of BSC (rather than a chemical fume hood) for primary
containment? Aerosolization can be a problem when preparing these types of
specimens. Further, our Infectious Disease Department is one of the largest
in the area such that we receive some of the more uncommon cases. I
understand that the Bloodborne Pathogens Standard, to an extent, dictates
the standards of practice. However, due to the fact that we have already
had some problems with one or two CJD cases, necrotizing fasciitis, TB,
etc., etc., what type of BSC is needed for work with these types of
unknowns?
2. Do research hospitals typically only utilize chemical fume hoods in
the following types of departments: Surgical Pathology, Histology, Cytology,
Autopsy Services, Dermatopathology and Neuropathology? If not, could you
let me know what other institutions are currently using?
3. Do research hospitals typically have Biosafety Committees or are
these committees only associated with the affiliated school of medicine?
4. Do research hospitals typically have biosafety programs and policies
for their laboratories (i.e. special procedures for handling different
organs and tissue specimens, required personal protective equipment, and
laboratory decontamination procedures?
5. Is it acceptable to place all "infectious waste" from these
laboratories into red bags (and without any kind of treatment), transport
them to a central location for transport to an offsite treatment facility?
Could you tell me of any specific implications this practice would have on
our Environmental Service (housekeeping) workers who pickup, collect, and
package the red bags? We have had incidents where workers were cut by
sharps that were placed in the wrong containers and workers who have been
splash when a red bag breaks open. However, no one knows what infectious
agents may actually be in the red bag involved, hence, these incidents are
usually not treated as anything major.
Any feedback that you have time to provide would be greatly appreciated.
=========================================================================
Date: Wed, 18 Aug 1999 15:38:08 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Heather H. Gonsoulin"
Subject: Re: BSC's in Research Hospitals
MIME-Version: 1.0
Content-Type: multipart/mixed; boundary="---- =_NextPart_000_01BEE98F.AE4E1320"
------ =_NextPart_000_01BEE98F.AE4E1320
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: quoted-printable
Along the same lines as the questions below, are non-research hospital =
labs going by the BMBL? Are they just using universal precautions, even =
when dealing with a blood specimen from an AIDS patient or HBV patient? =
These questions have come up at a local community hospital lately. =20
Thanks,
Heather
Heather H. Gonsoulin, RRA
Occupational Health and Safety Officer
USL-NIRC
( 318-482-0306 (fax) 318-373-0057
hah8377@usl.edu
-----Original Message-----
From: Stetz, Sharon [SMTP:Sharon.Stetz@]
Sent: Wednesday, 18 August, 1999 3:09 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: BSC's in Research Hospitals
I am fairly new to the biosafety-community, have learned quite alot =
already
since subscribing to this list at the end of June (1999), and have a
significant chemical safety background. With that out of the way, I =
have
inherited the task of assessing our various Pathology laboratories for
compliance with the accepted biosafety standards of practice. I work in =
a
large, urban, research hospital that has a significant Infectious =
Diseases
Department and treatment unit for both adults and pediatrics. I have =
been
reading the BMBL but still have a few basic questions and would really
appreciate some input.
1. Wouldn't Pathology laboratories that take specimen samples from
limbs and various organs to perform the requested diagnostics need to =
use at
least some type of BSC (rather than a chemical fume hood) for primary
containment? Aerosolization can be a problem when preparing these types =
of
specimens. Further, our Infectious Disease Department is one of the =
largest
in the area such that we receive some of the more uncommon cases. I
understand that the Bloodborne Pathogens Standard, to an extent, =
dictates
the standards of practice. However, due to the fact that we have =
already
had some problems with one or two CJD cases, necrotizing fasciitis, TB,
etc., etc., what type of BSC is needed for work with these types of
unknowns?
2. Do research hospitals typically only utilize chemical fume hoods =
in
the following types of departments: Surgical Pathology, Histology, =
Cytology,
Autopsy Services, Dermatopathology and Neuropathology? If not, could =
you
let me know what other institutions are currently using?
3. Do research hospitals typically have Biosafety Committees or are
these committees only associated with the affiliated school of medicine?
4. Do research hospitals typically have biosafety programs and =
policies
for their laboratories (i.e. special procedures for handling different
organs and tissue specimens, required personal protective equipment, and
laboratory decontamination procedures?
5. Is it acceptable to place all "infectious waste" from these
laboratories into red bags (and without any kind of treatment), =
transport
them to a central location for transport to an offsite treatment =
facility?
Could you tell me of any specific implications this practice would have =
on
our Environmental Service (housekeeping) workers who pickup, collect, =
and
package the red bags? We have had incidents where workers were cut by
sharps that were placed in the wrong containers and workers who have =
been
splash when a red bag breaks open. However, no one knows what =
infectious
agents may actually be in the red bag involved, hence, these incidents =
are
usually not treated as anything major.
Any feedback that you have time to provide would be greatly appreciated.
------ =_NextPart_000_01BEE98F.AE4E1320
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------ =_NextPart_000_01BEE98F.AE4E1320--
=========================================================================
Date: Wed, 18 Aug 1999 16:48:23 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Disposal of Refrigerators used for cadavers
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Our procedures,
Obtain an equipment disposal form equipment accounting(This is capital
equipment that comes off our official inventory).
The professor signs off.
The chair signs off.
We check for radiation, chemical and biological hazards then sign off.
Plant Services will remove the refrigerant then sign off.
Take it away.
Bob
>Our Anatomy department is replacing old refrigerators and freezers that
>were used to store cadavers and animal remains for many years.
>
>The company who is going to do the removal wants to know:
>
>1. Are there any special precautions they have to take.
>
>2. Can they dispose of these the normal way (like other appliances)?
>
>3. Is it only a chemical hazard or is it biological too at this point?
>
>4. Does it have to be decontaminated in some way?
>
>I would like to hear from those of you who have had experience in this area.
>
>
>Thanks for your input.
>
>Sincerely:
>
>Ninni Jacob, CHP
>Radiation and Biological Safety Officer
>Office of Risk Management
>Brown University - Box 1914
>164 Angell Street
>Providence, RI 02912
>
>Tel:401 863 1738
>Fax:401 863 7676
>
>email: Ninni_Jacob@brown.edu
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Wed, 18 Aug 1999 16:59:22 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: BSC's in Research Hospitals
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Yo Sharon!,
I'm a stones throw away. Call me anytime!
Bob
>I am fairly new to the biosafety-community, have learned quite alot already
>since subscribing to this list at the end of June (1999), and have a
>significant chemical safety background. With that out of the way, I have
>inherited the task of assessing our various Pathology laboratories for
>compliance with the accepted biosafety standards of practice. I work in a
>large, urban, research hospital that has a significant Infectious Diseases
>Department and treatment unit for both adults and pediatrics. I have been
>reading the BMBL but still have a few basic questions and would really
>appreciate some input.
>1. Wouldn't Pathology laboratories that take specimen samples from
>limbs and various organs to perform the requested diagnostics need to use at
>least some type of BSC (rather than a chemical fume hood) for primary
>containment? Aerosolization can be a problem when preparing these types of
>specimens. Further, our Infectious Disease Department is one of the largest
>in the area such that we receive some of the more uncommon cases. I
>understand that the Bloodborne Pathogens Standard, to an extent, dictates
>the standards of practice. However, due to the fact that we have already
>had some problems with one or two CJD cases, necrotizing fasciitis, TB,
>etc., etc., what type of BSC is needed for work with these types of
>unknowns?
>2. Do research hospitals typically only utilize chemical fume hoods in
>the following types of departments: Surgical Pathology, Histology, Cytology,
>Autopsy Services, Dermatopathology and Neuropathology? If not, could you
>let me know what other institutions are currently using?
>3. Do research hospitals typically have Biosafety Committees or are
>these committees only associated with the affiliated school of medicine?
>4. Do research hospitals typically have biosafety programs and policies
>for their laboratories (i.e. special procedures for handling different
>organs and tissue specimens, required personal protective equipment, and
>laboratory decontamination procedures?
>5. Is it acceptable to place all "infectious waste" from these
>laboratories into red bags (and without any kind of treatment), transport
>them to a central location for transport to an offsite treatment facility?
>Could you tell me of any specific implications this practice would have on
>our Environmental Service (housekeeping) workers who pickup, collect, and
>package the red bags? We have had incidents where workers were cut by
>sharps that were placed in the wrong containers and workers who have been
>splash when a red bag breaks open. However, no one knows what infectious
>agents may actually be in the red bag involved, hence, these incidents are
>usually not treated as anything major.
>
>Any feedback that you have time to provide would be greatly appreciated.
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Wed, 18 Aug 1999 14:23:15 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Gwenn Oki
Subject: Re[2]: Disposal of Refrigerators used for cadavers
MIME-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: 7bit
I would like to know how institutions are classifying research using lentiviral
vectors and required BSL. (There appears to be controversy as to whether this
requires BSL 2 or 3 containment.)
Thanks,
Gwenn Oki
City of Hope National Medical Center
Duarte, CA
=========================================================================
Date: Thu, 19 Aug 1999 09:05:29 +1000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ms Sonya Watson
Subject: Health surveillance
MIME-version: 1.0
Content-type: text/plain; charset="us-ascii"
We are currently investigating the dilemma of serum sampling and health
surveillance for individuals conducting research with Chlamydia pneumoniae.
The legislative documents available here do not provide a great deal of
guidance stating for general microbiological work "Consideration should be
given to collecting baseline serum samples from 'at-risk' personnel, to be
stored for future reference. Additional serum samples may be collected
periodically, depending on the risk of exposure to agents handled in the
laboratory. (AS 2243.3)"
Our dilemma, given that this is a ubiquitous organism and the majority of
the population has most probably been exposed at sometime during their
lifetime, is it worthwhile stipulating that researchers 'monitor for
symptoms and confirm via serology'? How do you distinguish between
probable occupational exposure and exposure from the "normal" environment?
Your views, and any guidance that could be provided would be greatly
appreciated.
Many thanks,
Sonya
************************************
Sonya Watson
Health and Safety Adviser (Biosafety)
School of Life Sciences
Queensland University of Technology
BRISBANE QLD AUSTRALIA
Tel: 61 7 3864 2917
Fax: 61 7 3864 1534
Email: s.watson@qut.edu.au
************************************
=========================================================================
Date: Wed, 18 Aug 1999 21:13:00 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Laura Newton
Subject: Re: BSC's in Research Hospitals
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Sharon, Here's a start: All employers are responsible for providing a safe
and healthful workplace for their employees.
I'm going to address question # 5 because that is the most concerning of
your questions. The others have merit also...
Each state has it's own infectious/medical waste regulations that specify
procedures for collecting and disposal of infectious waste. OSHA Bloodborne
Pathogens regulations also cover waste. Check with a biosafety professional
in your area on the regulations in force for your location.
Key concepts in waste collection anywhere are SHARPS CONTROL! Sharps must
be segregated at the point of generation and collected in rigid, leak-proof
sharps containers to avoid injury of those handling the waste. Red bags
should also be placed in a rigid container for transport within the
facility. Nalgene tubs or rigid cardboard burn boxes are typically used.
It sounds like you have a lot of biosafety issues. You may wish to attend
an in-depth biosafety course and/or the ABSA conference in October to get up
to speed. Check the ABSA web site
(If you put your e-mail address at the end of the message you send, people
can e-mail you directly without sending to the entire list)
Laura Newton
Newton Health and Safety Associates
newtonlb@
-----Original Message-----
From: Stetz, Sharon
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Wednesday, August 18, 1999 4:24 PM
Subject: BSC's in Research Hospitals
>I am fairly new to the biosafety-community, have learned quite alot already
>since subscribing to this list at the end of June (1999), and have a
>significant chemical safety background. With that out of the way, I have
>inherited the task of assessing our various Pathology laboratories for
>compliance with the accepted biosafety standards of practice. I work in a
>large, urban, research hospital that has a significant Infectious Diseases
>Department and treatment unit for both adults and pediatrics. I have been
>reading the BMBL but still have a few basic questions and would really
>appreciate some input.
>1. Wouldn't Pathology laboratories that take specimen samples from
>limbs and various organs to perform the requested diagnostics need to use
at
>least some type of BSC (rather than a chemical fume hood) for primary
>containment? Aerosolization can be a problem when preparing these types of
>specimens. Further, our Infectious Disease Department is one of the
largest
>in the area such that we receive some of the more uncommon cases. I
>understand that the Bloodborne Pathogens Standard, to an extent, dictates
>the standards of practice. However, due to the fact that we have already
>had some problems with one or two CJD cases, necrotizing fasciitis, TB,
>etc., etc., what type of BSC is needed for work with these types of
>unknowns?
>2. Do research hospitals typically only utilize chemical fume hoods in
>the following types of departments: Surgical Pathology, Histology,
Cytology,
>Autopsy Services, Dermatopathology and Neuropathology? If not, could you
>let me know what other institutions are currently using?
>3. Do research hospitals typically have Biosafety Committees or are
>these committees only associated with the affiliated school of medicine?
>4. Do research hospitals typically have biosafety programs and
policies
>for their laboratories (i.e. special procedures for handling different
>organs and tissue specimens, required personal protective equipment, and
>laboratory decontamination procedures?
>5. Is it acceptable to place all "infectious waste" from these
>laboratories into red bags (and without any kind of treatment), transport
>them to a central location for transport to an offsite treatment facility?
>Could you tell me of any specific implications this practice would have on
>our Environmental Service (housekeeping) workers who pickup, collect, and
>package the red bags? We have had incidents where workers were cut by
>sharps that were placed in the wrong containers and workers who have been
>splash when a red bag breaks open. However, no one knows what infectious
>agents may actually be in the red bag involved, hence, these incidents are
>usually not treated as anything major.
>
>Any feedback that you have time to provide would be greatly appreciated.
=========================================================================
Date: Wed, 18 Aug 1999 22:53:49 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Andres Marcel Mellado Rebolledo
Subject: unsubscribe
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This is a multi-part message in MIME format.
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=========================================================================
Date: Wed, 18 Aug 1999 20:25:19 -0400
Reply-To: jkeene@
Sender: A Biosafety Discussion List
From: "John H. Keene"
Subject: Re: BSC's in Research Hospitals
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
We need to be careful in how we define things. 29CFR1910:1030 OSHA
Bloodborne Pathogen Standard defines sharps containers as:
"Closable,puncture resistant, Leakproof on sides and bottoms, and Labeled or
color coded in accordance with paragraph (g)(1)(i)of this standard". The
containers do not have to be totally leak proof.
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Laura Newton
Sent: Wednesday, August 18, 1999 9:13 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: BSC's in Research Hospitals
Sharon, Here's a start: All employers are responsible for providing a safe
and healthful workplace for their employees.
I'm going to address question # 5 because that is the most concerning of
your questions. The others have merit also...
Each state has it's own infectious/medical waste regulations that specify
procedures for collecting and disposal of infectious waste. OSHA Bloodborne
Pathogens regulations also cover waste. Check with a biosafety professional
in your area on the regulations in force for your location.
Key concepts in waste collection anywhere are SHARPS CONTROL! Sharps must
be segregated at the point of generation and collected in rigid, leak-proof
sharps containers to avoid injury of those handling the waste. Red bags
should also be placed in a rigid container for transport within the
facility. Nalgene tubs or rigid cardboard burn boxes are typically used.
It sounds like you have a lot of biosafety issues. You may wish to attend
an in-depth biosafety course and/or the ABSA conference in October to get up
to speed. Check the ABSA web site
(If you put your e-mail address at the end of the message you send, people
can e-mail you directly without sending to the entire list)
Laura Newton
Newton Health and Safety Associates
newtonlb@
-----Original Message-----
From: Stetz, Sharon
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Wednesday, August 18, 1999 4:24 PM
Subject: BSC's in Research Hospitals
>I am fairly new to the biosafety-community, have learned quite alot already
>since subscribing to this list at the end of June (1999), and have a
>significant chemical safety background. With that out of the way, I have
>inherited the task of assessing our various Pathology laboratories for
>compliance with the accepted biosafety standards of practice. I work in a
>large, urban, research hospital that has a significant Infectious Diseases
>Department and treatment unit for both adults and pediatrics. I have been
>reading the BMBL but still have a few basic questions and would really
>appreciate some input.
>1. Wouldn't Pathology laboratories that take specimen samples from
>limbs and various organs to perform the requested diagnostics need to use
at
>least some type of BSC (rather than a chemical fume hood) for primary
>containment? Aerosolization can be a problem when preparing these types of
>specimens. Further, our Infectious Disease Department is one of the
largest
>in the area such that we receive some of the more uncommon cases. I
>understand that the Bloodborne Pathogens Standard, to an extent, dictates
>the standards of practice. However, due to the fact that we have already
>had some problems with one or two CJD cases, necrotizing fasciitis, TB,
>etc., etc., what type of BSC is needed for work with these types of
>unknowns?
>2. Do research hospitals typically only utilize chemical fume hoods in
>the following types of departments: Surgical Pathology, Histology,
Cytology,
>Autopsy Services, Dermatopathology and Neuropathology? If not, could you
>let me know what other institutions are currently using?
>3. Do research hospitals typically have Biosafety Committees or are
>these committees only associated with the affiliated school of medicine?
>4. Do research hospitals typically have biosafety programs and
policies
>for their laboratories (i.e. special procedures for handling different
>organs and tissue specimens, required personal protective equipment, and
>laboratory decontamination procedures?
>5. Is it acceptable to place all "infectious waste" from these
>laboratories into red bags (and without any kind of treatment), transport
>them to a central location for transport to an offsite treatment facility?
>Could you tell me of any specific implications this practice would have on
>our Environmental Service (housekeeping) workers who pickup, collect, and
>package the red bags? We have had incidents where workers were cut by
>sharps that were placed in the wrong containers and workers who have been
>splash when a red bag breaks open. However, no one knows what infectious
>agents may actually be in the red bag involved, hence, these incidents are
>usually not treated as anything major.
>
>Any feedback that you have time to provide would be greatly appreciated.
=========================================================================
Date: Wed, 18 Aug 1999 20:39:41 -0400
Reply-To: jkeene@
Sender: A Biosafety Discussion List
From: "John H. Keene"
Subject: Re: Disposal of Refrigerators used for cadavers
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
In most places, the lab supervisor or department supervisor should be the
individual responsible for decontamination of surplus equipment. They would
be the ones that should know what was used in/on the equipment and should be
able to decontaminate it.
Specifically with regard to biological hazards, the OSHA BBP std
(29CFR1910:1030) states that "Equipment which may become contaminataed with
blood or other potentially infectious materials shall be examined prior to
servicing or shipping and shall be decontaminated as necessary, unless the
employer can demonstrate that decontamination of such equipment or portions
of such equipment is not feasible. A readily observable label in accordance
with Paragraph (g)(1)(i)(H)shall be attached to the equipment stating which
portions remain contaminated. The employer shall ensure that this
information is conveyed to all affected employees, the servicing
representative, and or the manufacturer as appropriate, prir to handling
servicing, or shipping so that appropriate precautions will be taken.
Bottom line, determine if, and where the equipment could have been
contaminated and decontaminate before sending out for disposal. Any
tuberculocidal disinfectant used in accordance with the manufacturers
directions should be appropriate.
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Ninni Jacob
Sent: Wednesday, August 18, 1999 1:46 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Disposal of Refrigerators used for cadavers
Our Anatomy department is replacing old refrigerators and freezers that
were used to store cadavers and animal remains for many years.
The company who is going to do the removal wants to know:
1. Are there any special precautions they have to take.
2. Can they dispose of these the normal way (like other appliances)?
3. Is it only a chemical hazard or is it biological too at this point?
4. Does it have to be decontaminated in some way?
I would like to hear from those of you who have had experience in this area.
Thanks for your input.
Sincerely:
Ninni Jacob, CHP
Radiation and Biological Safety Officer
Office of Risk Management
Brown University - Box 1914
164 Angell Street
Providence, RI 02912
Tel:401 863 1738
Fax:401 863 7676
email: Ninni_Jacob@brown.edu
=========================================================================
Date: Wed, 18 Aug 1999 20:44:48 -0400
Reply-To: jkeene@
Sender: A Biosafety Discussion List
From: "John H. Keene"
Subject: Re: BSC's in Research Hospitals
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
All hospital labs should be following the BMBL at a minimum. Although this
document is not LAW, it is the standard of the industry with regard to the
handling of various human pathogens. Anyone not following the
recommendations in the BMBL is putting themselves at risk to serious
problems not only with regard to potential occupational exposure, but the
legal liability for those exposures and resultant illnesses.
-----Original Message-----
From: A Biosafety Discussion List [mailto:hah8377@usl.edu] On Behalf Of
Heather H. Gonsoulin
Sent: Wednesday, August 18, 1999 4:38 PM
To: 'A Biosafety Discussion List'
Subject: RE: BSC's in Research Hospitals
Along the same lines as the questions below, are non-research hospital labs
going by the BMBL? Are they just using universal precautions, even when
dealing with a blood specimen from an AIDS patient or HBV patient? These
questions have come up at a local community hospital lately.
Thanks,
Heather
Heather H. Gonsoulin, RRA
Occupational Health and Safety Officer
USL-NIRC
( 318-482-0306 (fax) 318-373-0057
-----Original Message-----
From: Stetz, Sharon [SMTP:Sharon.Stetz@]
Sent: Wednesday, 18 August, 1999 3:09 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: BSC's in Research Hospitals
I am fairly new to the biosafety-community, have learned quite alot already
since subscribing to this list at the end of June (1999), and have a
significant chemical safety background. With that out of the way, I have
inherited the task of assessing our various Pathology laboratories for
compliance with the accepted biosafety standards of practice. I work in a
large, urban, research hospital that has a significant Infectious Diseases
Department and treatment unit for both adults and pediatrics. I have been
reading the BMBL but still have a few basic questions and would really
appreciate some input.
1. Wouldn't Pathology laboratories that take specimen samples from
limbs and various organs to perform the requested diagnostics need to use at
least some type of BSC (rather than a chemical fume hood) for primary
containment? Aerosolization can be a problem when preparing these types of
specimens. Further, our Infectious Disease Department is one of the largest
in the area such that we receive some of the more uncommon cases. I
understand that the Bloodborne Pathogens Standard, to an extent, dictates
the standards of practice. However, due to the fact that we have already
had some problems with one or two CJD cases, necrotizing fasciitis, TB,
etc., etc., what type of BSC is needed for work with these types of
unknowns?
2. Do research hospitals typically only utilize chemical fume hoods in
the following types of departments: Surgical Pathology, Histology, Cytology,
Autopsy Services, Dermatopathology and Neuropathology? If not, could you
let me know what other institutions are currently using?
3. Do research hospitals typically have Biosafety Committees or are
these committees only associated with the affiliated school of medicine?
4. Do research hospitals typically have biosafety programs and policies
for their laboratories (i.e. special procedures for handling different
organs and tissue specimens, required personal protective equipment, and
laboratory decontamination procedures?
5. Is it acceptable to place all "infectious waste" from these
laboratories into red bags (and without any kind of treatment), transport
them to a central location for transport to an offsite treatment facility?
Could you tell me of any specific implications this practice would have on
our Environmental Service (housekeeping) workers who pickup, collect, and
package the red bags? We have had incidents where workers were cut by
sharps that were placed in the wrong containers and workers who have been
splash when a red bag breaks open. However, no one knows what infectious
agents may actually be in the red bag involved, hence, these incidents are
usually not treated as anything major.
Any feedback that you have time to provide would be greatly appreciated.
=========================================================================
Date: Thu, 19 Aug 1999 07:22:50 +0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Dicky Ng
Subject: unsubscribe
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> From: Richard Fink
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Job Announcement- Biological Safety
> Date: Thursday, August 19, 1999 3:46 AM
>
>
> >
> >Environmental Health & Safety Specialist
> >
> >Tulane University Medical Center, Department of Environmental Health &
> >Safety is seeking a qualified candidate to coordinate
> >the Infectious Disease and Biological Safety Program for Tulane
University.
> >
> >Individual is responsible for all occupational and environmental health
and
> >safety compliance with federal, state, and local
> >regulations as they relate to the program (OSHA Bloodborne and TB
> >Prevention Program and Recombinant DNA and Gene
> >Therapy Research). Will participate in EHS Emergency On-Call Program for
> >safety and health issues at Tulane.
> >
> >Bachelor's Degree in Biological Science required, (Microbiology,
> >Environmental or Occupational Science preferred). Minimum
> >of 3 years combined clinical/educational experience with emphasis on
> >infection control/infectious diseases desired. Safety
> >experience in laboratory safety, industrial hygiene,
> >occupational/environmental protection is a plus. Must possess excellent
> >written and verbal communication skills, computer skills required.
Strong
> >background in microbiology is essential. Professional
> >Bio-safety certification or eligibility is desirable.
> >
> >Qualified candidates should send resume along with cover letter and 3
> >references on or before September 30, 1999 to:
> >
> >Tulane University Medical Center
> >Human Resources
> >1430 Tulane Avenue EP-13
> >New Orleans, LA 70112
> >
> >AA/EOE
> >
> >
> >
> >
> >
> >
> >
> >
> Richard Fink, SM(NRM), CBSP
> Biosafty List Owner
> rfink@mit.edu
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----------> From: =
Richard Fink <rfink@MIT.EDU>> To: BIOSAFTY@MITVMA.MIT.EDU> Subject: Job Announcement- Biological =
Safety> Date: Thursday, August 19, 1999 3:46 AM> > =
> >> >Environmental Health & Safety =
Specialist> >> >Tulane University Medical Center, =
Department of Environmental Health &> >Safety is seeking a =
qualified candidate to coordinate> >the Infectious Disease and =
Biological Safety Program for Tulane University.> >> =
>Individual is responsible for all occupational and environmental =
health and> >safety compliance with federal, state, and =
local> >regulations as they relate to the program (OSHA =
Bloodborne and TB> >Prevention Program and Recombinant DNA and =
Gene> >Therapy Research). Will participate in EHS Emergency =
On-Call Program for> >safety and health issues at =
Tulane.> >> >Bachelor's Degree in Biological Science =
required, (Microbiology,> >Environmental or Occupational =
Science preferred). Minimum> >of 3 years combined =
clinical/educational experience with emphasis on> >infection =
control/infectious diseases desired. Safety> >experience in =
laboratory safety, industrial hygiene,> =
>occupational/environmental protection is a plus. Must possess =
excellent> >written and verbal communication skills, computer =
skills required. Strong> >background in microbiology is =
essential. Professional> >Bio-safety certification or =
eligibility is desirable.> >> >Qualified candidates =
should send resume along with cover letter and 3> >references =
on or before September 30, 1999 to:> >> >Tulane =
University Medical Center> >Human Resources> >1430 =
Tulane Avenue EP-13> >New Orleans, LA 70112> =
>> >AA/EOE> >> >> >> =
>> >> >> >> >> Richard =
Fink, SM(NRM), CBSP> Biosafty List Owner> rfink@mit.edu
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=========================================================================
Date: Thu, 19 Aug 1999 08:09:24 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Debra Hunt
Subject: Re: BSC's in Research Hospitals
Mime-Version: 1.0
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Hi, Sharon, welcome to the Biosafety community..my institution includes both
research labs in the university and clinical labs in the hospital. I can
honestly say that the clinical labs are generally in much better shape
(safety-wise) because of the restrictions placed on them in the hospital
context. For example, clinical labs will fall under either the JCAHO (Joint
Commission for Accreditation of Healthcare Organizations) or the CAP (College of
American Pathologists). Both will require compliance with the OSHA Bloodborne
Pathogen Standard at minimum, and in most cases have even stricter requirements.
Because of this, all our clinical labs are compliant with Biosafety Level 2
facilities and practices. If you also have a Clinical Microbiology Lab that
cultures and diagnoses TB, BSL 3 is necessary.
Basically, BSCs are available for labs who handle clnical specimens and may
perform procedures where splashes or aerosolization is expected, or where
specimens potentially containing M. tb or other aerosol-transmitted organisms
may be handled. CJD is another story. Take a look at the latest edition of the
BMBL for special decontamination for this agent. We certainly try to label all
suspected CJD cases that go to the lab, and use different decontamination
procedures. Otherwise, Standard (Universal) Precautions are used.
I would suggest getting a copy of the NCCLS (National Committee for Clinical
Laboratory Standards) document, "Protection of Laboratory Workers from
Infectious Disease Transmitted by Blood, Body Fluids, and Tissue", M29-A.
This is a nice compilation of clinical lab-specific recommendations.
Hope this helps..
Debra L. Hunt, DrPH, CBSP
Director, Biological Safety
Duke University and Duke University Health Systems
Durham, NC
hunt0009@mc.duke.edu
919-684-8822; fax: 919-681-7509
=========================================================================
Date: Thu, 19 Aug 1999 08:22:39 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Stetz, Sharon"
Subject: BSCs in Research Hospitals
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
For those who wish to comment on the questions regarding BSCs in research
hospitals, it was politely pointed out that I omitted a direct emailing
address. Thankyou for this suggestion, and I can be reached at the
following address: Sharon.Stetz@
=========================================================================
Date: Thu, 19 Aug 1999 09:10:39 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Al Jin
Subject: Re: BSC's in Research Hospitals
In-Reply-To:
Mime-Version: 1.0
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To Everyone,
Please make sure you also comply with the definition as stated in your
State OSHA regs (if you have one). In most cases they are the same but in
some cases, the regs may be more stringent.
Alfred Jin, BSO, IH, MS, CBSP, M(ASCP), BSS(ASM), CM(ACM),
Hazards Control Department, Lawrence Livermore National Laboratory,
7000 East Avenue MS-289, Livermore, CA 94550,
Phone:925 423-7385, Fax:423-1086,
Jin2@
>We need to be careful in how we define things. 29CFR1910:1030 OSHA
>Bloodborne Pathogen Standard defines sharps containers as:
>"Closable,puncture resistant, Leakproof on sides and bottoms, and Labeled or
>color coded in accordance with paragraph (g)(1)(i)of this standard". The
>containers do not have to be totally leak proof.
>
>-----Original Message-----
>From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
>Behalf Of Laura Newton
>Sent: Wednesday, August 18, 1999 9:13 PM
>To: BIOSAFTY@MITVMA.MIT.EDU
>Subject: Re: BSC's in Research Hospitals
>
>
>Sharon, Here's a start: All employers are responsible for providing a safe
>and healthful workplace for their employees.
>
>I'm going to address question # 5 because that is the most concerning of
>your questions. The others have merit also...
>
>Each state has it's own infectious/medical waste regulations that specify
>procedures for collecting and disposal of infectious waste. OSHA Bloodborne
>Pathogens regulations also cover waste. Check with a biosafety professional
>in your area on the regulations in force for your location.
>
>Key concepts in waste collection anywhere are SHARPS CONTROL! Sharps must
>be segregated at the point of generation and collected in rigid, leak-proof
>sharps containers to avoid injury of those handling the waste. Red bags
>should also be placed in a rigid container for transport within the
>facility. Nalgene tubs or rigid cardboard burn boxes are typically used.
>
>It sounds like you have a lot of biosafety issues. You may wish to attend
>an in-depth biosafety course and/or the ABSA conference in October to get up
>to speed. Check the ABSA web site
>
>(If you put your e-mail address at the end of the message you send, people
>can e-mail you directly without sending to the entire list)
>
>Laura Newton
>Newton Health and Safety Associates
>newtonlb@
>
>
>
>-----Original Message-----
>From: Stetz, Sharon
>To: BIOSAFTY@MITVMA.MIT.EDU
>Date: Wednesday, August 18, 1999 4:24 PM
>Subject: BSC's in Research Hospitals
>
>
>>I am fairly new to the biosafety-community, have learned quite alot already
>>since subscribing to this list at the end of June (1999), and have a
>>significant chemical safety background. With that out of the way, I have
>>inherited the task of assessing our various Pathology laboratories for
>>compliance with the accepted biosafety standards of practice. I work in a
>>large, urban, research hospital that has a significant Infectious Diseases
>>Department and treatment unit for both adults and pediatrics. I have been
>>reading the BMBL but still have a few basic questions and would really
>>appreciate some input.
>>1. Wouldn't Pathology laboratories that take specimen samples from
>>limbs and various organs to perform the requested diagnostics need to use
>at
>>least some type of BSC (rather than a chemical fume hood) for primary
>>containment? Aerosolization can be a problem when preparing these types of
>>specimens. Further, our Infectious Disease Department is one of the
>largest
>>in the area such that we receive some of the more uncommon cases. I
>>understand that the Bloodborne Pathogens Standard, to an extent, dictates
>>the standards of practice. However, due to the fact that we have already
>>had some problems with one or two CJD cases, necrotizing fasciitis, TB,
>>etc., etc., what type of BSC is needed for work with these types of
>>unknowns?
>>2. Do research hospitals typically only utilize chemical fume hoods in
>>the following types of departments: Surgical Pathology, Histology,
>Cytology,
>>Autopsy Services, Dermatopathology and Neuropathology? If not, could you
>>let me know what other institutions are currently using?
>>3. Do research hospitals typically have Biosafety Committees or are
>>these committees only associated with the affiliated school of medicine?
>>4. Do research hospitals typically have biosafety programs and
>policies
>>for their laboratories (i.e. special procedures for handling different
>>organs and tissue specimens, required personal protective equipment, and
>>laboratory decontamination procedures?
>>5. Is it acceptable to place all "infectious waste" from these
>>laboratories into red bags (and without any kind of treatment), transport
>>them to a central location for transport to an offsite treatment facility?
>>Could you tell me of any specific implications this practice would have on
>>our Environmental Service (housekeeping) workers who pickup, collect, and
>>package the red bags? We have had incidents where workers were cut by
>>sharps that were placed in the wrong containers and workers who have been
>>splash when a red bag breaks open. However, no one knows what infectious
>>agents may actually be in the red bag involved, hence, these incidents are
>>usually not treated as anything major.
>>
>>Any feedback that you have time to provide would be greatly appreciated.
=========================================================================
Date: Thu, 19 Aug 1999 10:05:31 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Al Jin
Subject: Re: Health surveillance
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/enriched; charset="us-ascii"
Sonya,
As I understand your question, you are asking (As we all often do) "Is
there any value added to performing serum
banking?". When exposure incident occurs, one collects as much
epidemiological information possible to determine the root cause for
exposure.
As I wipe the dust off my immunology/serology text books, individual or
group immunological profiles have been a valuable tool in determining
the extent of an exposure incident. A rise in the titer between the
acute and convalescent blood sample would indicate recent exposure to a
specific antigen. A steady titer in both samples may indicate past
exposure. In your case it will be a rise in titer to the Chlamydia
pneumoniae antigen(s). The types of antibodies (IgM, IgG, IgE) would
also help classify the types (immediate or delayed) of immune response
(aka Type I, II, III, IV).
Your question regarding ubiquitous organism in the population is a
difficult one to answer should an incident involve only one sole
individual. However, (excluding a company picnic) if more employees
begin to show evidence of symptoms and then showed positive
sero-conversion, then the data would be pretty obvious. You have a
problem.
In the situation where you choose not to perform serum banking, this
piece of information may be missed. Other factors such as symptoms may
be used as an indicator but it is never definitive enough to indicate
the presence of a problem and it make your job alittle harder.
At our site, we have a voluntary serum banking policy for employee
working with human blood. The employee's blood is collected by trained
phlebotomists at our Health Service Department. The serum is separated
from the RBC and stored frozen by each department. This show
departmental ownership to safety. Participation is voluntary since an
employer cannot force an employee to give blood or to get a vaccine.
The serum is banked until needed.
It is my professional opinon that a good medical surveillance program
would involve a proactive annually screening program by serology
testing of high risk employees for a specific antigen. Postive
sero-conversion would be an indicator for a documented exposure and the
information is now in the employee medical records. The employee can be
interview to determine if the exposure was occupational related or not.
In closing, it is my professional opinon that yes, there is value added
when banking of "high risk employees" serum for a specific antigen. And
yes, there is a lot more value added when each sample collected is
tested and employees are truly monitored.
Alfred Jin, BSO, IH, CBSP, M(ASCP), BSS(ASM), CM(ACM),
Hazards Control Department,
Lawrence Livermore National Laboratory,
7000 East Avenue MS-289, Livermore, CA 94550,
Phone:925 423-7385, Fax:423-1086,
Jin2@
>We are currently investigating the dilemma of serum sampling and
health
>surveillance for individuals conducting research with Chlamydia
pneumoniae.
> The legislative documents available here do not provide a great deal
of
>guidance stating for general microbiological work "Consideration
should be
>given to collecting baseline serum samples from 'at-risk' personnel,
to be
>stored for future reference. Additional serum samples may be
collected
>periodically, depending on the risk of exposure to agents handled in
the
>laboratory. (AS 2243.3)"
>
>Our dilemma, given that this is a ubiquitous organism and the majority
of
>the population has most probably been exposed at sometime during
their
>lifetime, is it worthwhile stipulating that researchers 'monitor for
>symptoms and confirm via serology'? How do you distinguish between
>probable occupational exposure and exposure from the "normal"
environment?
>
>Your views, and any guidance that could be provided would be greatly
>appreciated.
>
>Many thanks,
>Sonya
>
> ************************************
> Sonya Watson
> Health and Safety Adviser (Biosafety)
> School of Life Sciences
> Queensland University of Technology
> BRISBANE QLD AUSTRALIA
>
> Tel: 61 7 3864 2917
> Fax: 61 7 3864 1534
> Email: s.watson@qut.edu.au
>
> ************************************
=========================================================================
Date: Thu, 19 Aug 1999 15:05:32 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Heather H. Gonsoulin"
Subject: Re: Health surveillance
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Another question with regards to serum banking. How long do you keep =
the serum? We are beginning to develop a problem with freezer space, =
and the question has been asked "At what point can we get rid of old =
serum?". Do any of you have any insight into this dilemma? Your help =
is greatly appreciated.
Thanks,
Heather
Heather H. Gonsoulin, RRA
Occupational Health and Safety Officer
USL-NIRC
( 318-482-0306 (fax) 318-373-0057
hah8377@usl.edu
-----Original Message-----
From: Al Jin [SMTP:jin2@]
Sent: Thursday, 19 August, 1999 12:06 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: Health surveillance
Sonya,
As I understand your question, you are asking (As we all often do) "Is
there any value added to performing serum
banking?". When exposure incident occurs, one collects as much
epidemiological information possible to determine the root cause for
exposure.
As I wipe the dust off my immunology/serology text books, individual or
group immunological profiles have been a valuable tool in determining
the extent of an exposure incident. A rise in the titer between the
acute and convalescent blood sample would indicate recent exposure to a
specific antigen. A steady titer in both samples may indicate past
exposure. In your case it will be a rise in titer to the Chlamydia
pneumoniae antigen(s). The types of antibodies (IgM, IgG, IgE) would
also help classify the types (immediate or delayed) of immune response
(aka Type I, II, III, IV).
Your question regarding ubiquitous organism in the population is a
difficult one to answer should an incident involve only one sole
individual. However, (excluding a company picnic) if more employees
begin to show evidence of symptoms and then showed positive
sero-conversion, then the data would be pretty obvious. You have a
problem.
In the situation where you choose not to perform serum banking, this
piece of information may be missed. Other factors such as symptoms may
be used as an indicator but it is never definitive enough to indicate
the presence of a problem and it make your job alittle harder.
At our site, we have a voluntary serum banking policy for employee
working with human blood. The employee's blood is collected by trained
phlebotomists at our Health Service Department. The serum is separated
from the RBC and stored frozen by each department. This show
departmental ownership to safety. Participation is voluntary since an
employer cannot force an employee to give blood or to get a vaccine.
The serum is banked until needed.
It is my professional opinon that a good medical surveillance program
would involve a proactive annually screening program by serology
testing of high risk employees for a specific antigen. Postive
sero-conversion would be an indicator for a documented exposure and the
information is now in the employee medical records. The employee can be
interview to determine if the exposure was occupational related or not.
In closing, it is my professional opinon that yes, there is value added
when banking of "high risk employees" serum for a specific antigen. And
yes, there is a lot more value added when each sample collected is
tested and employees are truly monitored.
Alfred Jin, BSO, IH, CBSP, M(ASCP), BSS(ASM), CM(ACM),
Hazards Control Department,
Lawrence Livermore National Laboratory,
7000 East Avenue MS-289, Livermore, CA 94550,
Phone:925 423-7385, Fax:423-1086,
Jin2@
>We are currently investigating the dilemma of serum sampling and
health
>surveillance for individuals conducting research with Chlamydia
pneumoniae.
> The legislative documents available here do not provide a great deal
of
>guidance stating for general microbiological work "Consideration
should be
>given to collecting baseline serum samples from 'at-risk' personnel,
to be
>stored for future reference. Additional serum samples may be
collected
>periodically, depending on the risk of exposure to agents handled in
the
>laboratory. (AS 2243.3)"
>
>Our dilemma, given that this is a ubiquitous organism and the majority
of
>the population has most probably been exposed at sometime during
their
>lifetime, is it worthwhile stipulating that researchers 'monitor for
>symptoms and confirm via serology'? How do you distinguish between
>probable occupational exposure and exposure from the "normal"
environment?
>
>Your views, and any guidance that could be provided would be greatly
>appreciated.
>
>Many thanks,
>Sonya
>
> ************************************
> Sonya Watson
> Health and Safety Adviser (Biosafety)
> School of Life Sciences
> Queensland University of Technology
> BRISBANE QLD AUSTRALIA
>
> Tel: 61 7 3864 2917
> Fax: 61 7 3864 1534
> Email: s.watson@qut.edu.au
>
> ************************************
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------ =_NextPart_000_01BEEA54.4A181FC0--
=========================================================================
Date: Thu, 19 Aug 1999 14:02:28 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Al Jin
Subject: Re: Health surveillance
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Heather,
Storage space is always a problem when one is serum banking. Since the
serum is not medical information (ie test results for medical records),
there is no legal precedence to keep the material for a specific time, it's
a judgement call.
Whether you are serum banking for 1 antigen or unknown antigens, I (as the
Serum Bank Manager) would periodically review and update the list of names
on the roster. I would give priority space to those who are currently
at-risk employees, then to those who are not-at-risk employees but still
working in the lab, then to employees who retire from laboratory work but
are still employees and finally, to non-employees. After a reasonable time
has past, I would dispose of those sera that are in the last category.
I realize this takes a litle bit of work on our part but as I mentioned
earlier, the best medical surveillance program would be a program that
proactively screens employees for a specific antigen and reviews the
analytical data.
Alfred Jin, BSO, IH, MS, CBSP, M(ASCP), BSS(ASM), CM(ACM),
Hazards Control Department,
Lawrence Livermore National Laboratory,
7000 East Avenue MS-289, Livermore, CA 94550,
Phone:925 423-7385, Fax:423-1086,
Jin2@
>Another question with regards to serum banking. How long do you keep the
>serum? We are beginning to develop a problem with freezer space, and the
>question has been asked "At what point can we get rid of old serum?". Do
>any of you have any insight into this dilemma? Your help is greatly
>appreciated.
>Thanks,
>Heather
>
>Heather H. Gonsoulin, RRA
>Occupational Health and Safety Officer
>USL-NIRC
>( 318-482-0306 (fax) 318-373-0057
>hah8377@usl.edu
>
>
>-----Original Message-----
>From: Al Jin [SMTP:jin2@]
>Sent: Thursday, 19 August, 1999 12:06 PM
>To: BIOSAFTY@MITVMA.MIT.EDU
>Subject: Re: Health surveillance
>
>Sonya,
>
>
>As I understand your question, you are asking (As we all often do) "Is
>there any value added to performing serum
>banking?". When exposure incident occurs, one collects as much
>epidemiological information possible to determine the root cause for
>exposure.
>
>
>As I wipe the dust off my immunology/serology text books, individual or
>group immunological profiles have been a valuable tool in determining
>the extent of an exposure incident. A rise in the titer between the
>acute and convalescent blood sample would indicate recent exposure to a
>specific antigen. A steady titer in both samples may indicate past
>exposure. In your case it will be a rise in titer to the Chlamydia
>pneumoniae antigen(s). The types of antibodies (IgM, IgG, IgE) would
>also help classify the types (immediate or delayed) of immune response
>(aka Type I, II, III, IV).
>
>
>Your question regarding ubiquitous organism in the population is a
>difficult one to answer should an incident involve only one sole
>individual. However, (excluding a company picnic) if more employees
>begin to show evidence of symptoms and then showed positive
>sero-conversion, then the data would be pretty obvious. You have a
>problem.
>
>
>In the situation where you choose not to perform serum banking, this
>piece of information may be missed. Other factors such as symptoms may
>be used as an indicator but it is never definitive enough to indicate
>the presence of a problem and it make your job alittle harder.
>
>
>At our site, we have a voluntary serum banking policy for employee
>working with human blood. The employee's blood is collected by trained
>phlebotomists at our Health Service Department. The serum is separated
>>from the RBC and stored frozen by each department. This show
>departmental ownership to safety. Participation is voluntary since an
>employer cannot force an employee to give blood or to get a vaccine.
>The serum is banked until needed.
>
>
>It is my professional opinon that a good medical surveillance program
>would involve a proactive annually screening program by serology
>testing of high risk employees for a specific antigen. Postive
>sero-conversion would be an indicator for a documented exposure and the
>information is now in the employee medical records. The employee can be
>interview to determine if the exposure was occupational related or not.
>
>
>
>In closing, it is my professional opinon that yes, there is value added
>when banking of "high risk employees" serum for a specific antigen. And
>yes, there is a lot more value added when each sample collected is
>tested and employees are truly monitored.
>
>
>
>
>Alfred Jin, BSO, IH, CBSP, M(ASCP), BSS(ASM), CM(ACM),
>
>Hazards Control Department,
>
>Lawrence Livermore National Laboratory,
>
>7000 East Avenue MS-289, Livermore, CA 94550,
>
>Phone:925 423-7385, Fax:423-1086,
>
>Jin2@
>
>
>
>
>
>
>>We are currently investigating the dilemma of serum sampling and
>health
>
>>surveillance for individuals conducting research with Chlamydia
>pneumoniae.
>
>> The legislative documents available here do not provide a great deal
>of
>
>>guidance stating for general microbiological work "Consideration
>should be
>
>>given to collecting baseline serum samples from 'at-risk' personnel,
>to be
>
>>stored for future reference. Additional serum samples may be
>collected
>
>>periodically, depending on the risk of exposure to agents handled in
>the
>
>>laboratory. (AS 2243.3)"
>
>>
>
>>Our dilemma, given that this is a ubiquitous organism and the majority
>of
>
>>the population has most probably been exposed at sometime during
>their
>
>>lifetime, is it worthwhile stipulating that researchers 'monitor for
>
>>symptoms and confirm via serology'? How do you distinguish between
>
>>probable occupational exposure and exposure from the "normal"
>environment?
>
>>
>
>>Your views, and any guidance that could be provided would be greatly
>
>>appreciated.
>
>>
>
>>Many thanks,
>
>>Sonya
>
>>
>
>> ************************************
>
>> Sonya Watson
>
>> Health and Safety Adviser (Biosafety)
>
>> School of Life Sciences
>
>> Queensland University of Technology
>
>> BRISBANE QLD AUSTRALIA
>
>>
>
>> Tel: 61 7 3864 2917
>
>> Fax: 61 7 3864 1534
>
>> Email: s.watson@qut.edu.au
>
>>
>
>> ************************************
=========================================================================
Date: Thu, 19 Aug 1999 15:04:20 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Gwenn Oki
Subject: LENTIVIRAL VECTORS
MIME-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: 7bit
SORRY FOR SEEMING MIX-UP. BUT IN MY ELECTRONIC HASTE TO GET THIS INQUIRY OUT, I
HIT THE REPLY AND SEND BUTTONS, BUT DID NOT CHANGE THE SUBJECT SECTION OF THIS
E-MAIL WHEN I CREATED AND SENT THE FOLLOWING QUERY. I WOULD APPRECIATE A
RESPONSE. THANKS,
GWENN OKI
____________________Forward Header_____________________
Subject: Re[2]: Disposal of Refrigerators used for cadavers
Author: Gwenn Oki
Date: 8/18/99 2:23 PM
I would like to know how institutions are classifying research using lentiviral
vectors and required BSL. (There appears to be controversy as to whether this
requires BSL 2 or 3 containment.)
Thanks,
Gwenn Oki
City of Hope National Medical Center
Duarte, CA
=========================================================================
Date: Fri, 20 Aug 1999 16:59:48 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Noel Neighbor
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
We are looking into what we will have to do to work out the details of
compliance with 40 CFR Part 160 in order to do disinfectant efficacy
testing for companies that need EPA approval for label claims. Does
anyone know of a lab certification business that may be able to help us
with the details associated with equipment certification, standard
operating procedures, record keeping, and the establishing of acceptable
quality assurance?
Noel Neighbor,
Research Associate and Facility Manager Poultry Health Research Laboratory
Center of Excellence for Poultry Science
University of Arkansas
Fayetteville, Arkansas 72701
(501) 575-8493
(501) 575-8490 Fax
=========================================================================
Date: Mon, 23 Aug 1999 09:49:47 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Leonard J. Borzynski"
Subject: Read: BSC's in Research Hospitals
Your message
To: 'A Biosafety Discussion List'
Cc:
Subject: RE: BSC's in Research Hospitals
Sent: Wed, 18 Aug 1999 16:38:08 -0400
was read on Mon, 23 Aug 1999 10:09:00 -0400
=========================================================================
Date: Mon, 23 Aug 1999 16:06:18 +0200
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Giovanni Ferraiolo ICGEB BIOSAFETY
Organization: International Centre for Genetic Engineering and Biotechnology
[]
Subject: *** ICGEB BIOSAFETY NEWS - AUGUST 1999 ***
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
____________________________________________________
*** ICGEB BIOSAFETY NEWS - AUGUST 1999 ***
____________________________________________________
This message is to keep you updated with the news concerning
the ICGEB Biosafety WebPages and the activity of the Centre
in this issue. If you DO NOT want to receive further
messages, just REPLY typing "unsubscribe" in the "Subject"
field and your name will be automatically deleted from the
mailing list. ICGEB apologizes for any inconvenience due to
undesired posting.
____________________________________________________
TWO BIOSAFETY WORKSHOPS IN THE ICGEB YEAR 2000 CALENDAR
Two different workshops on biosafety and risk assessment for
the environmental release of genetically modified organisms
(GMOs) have been scheduled within the Year 2000 ICGEB
Calendar of Activities:
*** 27-31 March 2000, Trieste, ITALY. Workshop:
"Biosafety 1. Science and Policy in Risk Assessment of
Transgenic Organisms: A Case Study Approach".
Organizers: George Tzotzos, UNIDO, Vienna, Austria; Gilbert
Howe, University of Bristol, UK; Giovanni Ferraiolo, ICGEB,
Trieste, Italy.
*** 3-8 April 2000, Florence, ITALY. Workshop: "Biosafety
2. Advanced Research and Procedures: Case Studies for
Designated Experts".
Organizers: Marcello Broggio, Istituto Agronomico per
l'Oltremare, Florence, Italy; Giovanni Ferraiolo, ICGEB,
Trieste, Italy.
Requests for information and applications directly to:
Ms. Micaela Di Blas, ICGEB, Padriciano 99, 34012 Trieste,
Italy. Tel: +39-040-3757333; Fax: +39-040-226555;
mailto:courses@icgeb.trieste.it
* Biosafety 1: The workshop aims to offer a comprehensive
approach to biosafety and is directed to scientists
actively involved in biological risk assessment and/or
biotechnology regulations. ICGEB is dealing with biosafety
since its inception and since 1991 the Centre organizes
annual workshops on this issue attended, to date, from over
500 scientists involved in biosafety issues in more than 50
different countries.
* Biosafety 2: Following the suggestions of many biosafety
focal-points and resource persons we have added, in our
agenda for the year 2000, an advanced workshop, directed
exclusively to officers in Governmental Agencies and/or
designated experts, working in the area of risk assessment
of GMOs at official level (governments, scientific
institutions, private sector etc.). This second workshop
aims to offer to scientists with advanced expertise in risk
assessment and/or biosafety regulative framework a dedicated
forum for advanced discussion and information sharing
The general approach in both workshops will focus, with
special emphasis, on case study presentations and discussion
for the environmental release of genetically modified
organisms (GMOs). Detailed programs will be distributed when
available.
Preference will be given to applicants from developing
countries. A limited number of grants will be available for
scientists from ICGEB Member Countries. These grants will
cover accommodation meals and incidentals for the duration
of the course; travel is NOT funded. Registration for the
Workshop is limited to 30 participants.
Note . ICGEB is looking for Sponsors / Co-sponsors /
Contributors in order to recognize contributions toward
travel expenses of the participants. Interested
organizations may wish to contact the ICGEB
Secretariat in due time to facilitate the widest
participation to both workshops.
____________________________________________________
For more information on ICGEB biosafety activities, please
contact:
Giovanni Ferraiolo - mailto:biosafe@icgeb.trieste.it
ICGEB Biosafety, Programme Officer,
International Centre for Genetic Engineering and
Biotechnology. Area Science Park, Padriciano 99 I-34012
Trieste (Italy). Tel: +39 040 3757364 - Fax: +39 040 226555
ICGEB Biosafety WebPages:
____________________________________________________
The International Centre for Genetic Engineering and
Biotechnology (ICGEB) is an autonomous, international,
intergovernmental organization formed by 61 signatory
countries of which 43 are full Member States. The Centre is
dedicated to advanced research and training in molecular
biology and biotechnology. The mandate of the ICGEB is to
promote the safe use of biotechnology world-wide and with
special regard to the needs of the developing world. Further
information on the ICGEB could be found on its web pages at
____________________________________________________
mlt2
=========================================================================
Date: Mon, 23 Aug 1999 09:35:35 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Scott Rusk
Subject: *** ICGEB BIOSAFETY NEWS - AUGUST 1999 *** -Reply
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: quoted-printable
"unsubscribe"
=========================================================================
Date: Mon, 23 Aug 1999 16:17:32 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Paul Jennette
Subject: formaldehyde fumigation indicator
Mime-Version: 1.0
Content-Type: multipart/alternative;
boundary="=====================_33524098==_.ALT"
--=====================_33524098==_.ALT
Content-Type: text/plain; charset="us-ascii"
Has anyone had experience with a non-biological indicator to use with
formaldehyde fumigation? We are looking for something similar to autoclave
tape that would give an immediate response.
Thanks in advance for your comments.
J. Paul Jennette, P.E.
Biosafety Engineer
Cornell University
College of Veterinary Medicine
Biosafety Program
S3-010 Schurman Hall, Box 38 (607) 253-4227
Ithaca, New York 14853-6401 fax -3723
--=====================_33524098==_.ALT
Content-Type: text/html; charset="us-ascii"
Has anyone had experience with a non-biological indicator to
use with formaldehyde fumigation? We are looking for something
similar to autoclave tape that would give an immediate response.
Thanks in advance for your comments.
J. Paul Jennette, P.E.
Biosafety Engineer
Cornell University
College of Veterinary Medicine
Biosafety Program
S3-010 Schurman Hall, Box
38 (607)
253-4227
Ithaca, New York
14853-6401 fax
-3723
--=====================_33524098==_.ALT--
=========================================================================
Date: Tue, 24 Aug 1999 11:50:49 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: Re: formaldehyde fumigation indicator
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Dear Paul
I had some experience on a chemical indicator (i.e. color change in the
indicator paper). But I am not satisfied with this because of the
interference and the short storage life. The on-line machine can only
measure the concentration upto 100 ppm. So I think it is not suitable to
monitor the fumigation over several thousand ppm. The bacillus spore is the
only indicator that I can confirm the completeness of fumigation now.
YK Wan
Safety Officer, CUHK
At 04:17 PM 8/23/99 -0400, you wrote:
>
>Has anyone had experience with a non-biological indicator to use with
>formaldehyde fumigation? We are looking for something similar to autoclave
>tape that would give an immediate response.
>Thanks in advance for your comments.
>
>
>
>
>J. Paul Jennette, P.E.
>Biosafety Engineer
>Cornell University
>College of Veterinary Medicine
>Biosafety Program
>S3-010 Schurman Hall, Box 38 (607) 253-4227
>Ithaca, New York 14853-6401 fax -3723
>
>Has anyone had experience with a non-biological indicator to
>use with formaldehyde fumigation? We are looking for something
>similar to autoclave tape that would give an immediate response.
>Thanks in advance for your comments.
>
>
>
>
>
>
>J. Paul Jennette, P.E.
>Biosafety Engineer
>Cornell University
>College of Veterinary Medicine
>Biosafety Program
>S3-010 Schurman Hall, Box
>38 &nb
sp; (607)
>253-4227
>Ithaca, New York
>14853-6401 &
nbsp; fax
;
>-3723
>
>
>
>
Please reply when you receive the message. Thank you.
***** Yu Kwan WAN,
***** Safety Officer
***** The Chinese University of Hong Kong
***** Shatin, NT, Hong Kong
***** Email:
***** ulsoykwan@cuhk.edu.hk
***** ulsoykwan@
***** ulsoykwan@
***** ulsoykwan@
=========================================================================
Date: Tue, 24 Aug 1999 08:07:11 +0300
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: EITAN
Subject: Re: *** ICGEB BIOSAFETY NEWS - AUGUST 1999 ***
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
-----Original Message-----
From: Giovanni Ferraiolo ICGEB BIOSAFETY
To: BIOSAFTY@MITVMA.MIT.EDU
Date: =E9=E5=ED =F9=F0=E9 23 =E0=E5=E2=E5=F1=E8 1999 17:20
Subject: *** ICGEB BIOSAFETY NEWS - AUGUST 1999 ***
>____________________________________________________
>
> *** ICGEB BIOSAFETY NEWS - AUGUST 1999 ***
>
>____________________________________________________
>
>This message is to keep you updated with the news concerning
>the ICGEB Biosafety WebPages and the activity of the Centre
>in this issue. If you DO NOT want to receive further
>messages, just REPLY typing "unsubscribe" in the "Subject"
>field and your name will be automatically deleted from the
>mailing list. ICGEB apologizes for any inconvenience due to
>undesired posting.
>____________________________________________________
>
>TWO BIOSAFETY WORKSHOPS IN THE ICGEB YEAR 2000 CALENDAR
>
>Two different workshops on biosafety and risk assessment for
>the environmental release of genetically modified organisms
>(GMOs) have been scheduled within the Year 2000 ICGEB
>Calendar of Activities:
>
> *** 27-31 March 2000, Trieste, ITALY. Workshop:
>"Biosafety 1. Science and Policy in Risk Assessment of
>Transgenic Organisms: A Case Study Approach".
>Organizers: George Tzotzos, UNIDO, Vienna, Austria; Gilbert
>Howe, University of Bristol, UK; Giovanni Ferraiolo, ICGEB,
>Trieste, Italy.
>
> *** 3-8 April 2000, Florence, ITALY. Workshop: "Biosafety
>2. Advanced Research and Procedures: Case Studies for
>Designated Experts".
>Organizers: Marcello Broggio, Istituto Agronomico per
>l'Oltremare, Florence, Italy; Giovanni Ferraiolo, ICGEB,
>Trieste, Italy.
>
> Requests for information and applications directly to:
>Ms. Micaela Di Blas, ICGEB, Padriciano 99, 34012 Trieste,
>Italy. Tel: +39-040-3757333; Fax: +39-040-226555;
>mailto:courses@icgeb.trieste.it
>
>
>
>* Biosafety 1: The workshop aims to offer a comprehensive
>approach to biosafety and is directed to scientists
>actively involved in biological risk assessment and/or
>biotechnology regulations. ICGEB is dealing with biosafety
>since its inception and since 1991 the Centre organizes
>annual workshops on this issue attended, to date, from over
>500 scientists involved in biosafety issues in more than 50
>different countries.
>
>* Biosafety 2: Following the suggestions of many biosafety
>focal-points and resource persons we have added, in our
>agenda for the year 2000, an advanced workshop, directed
>exclusively to officers in Governmental Agencies and/or
>designated experts, working in the area of risk assessment
>of GMOs at official level (governments, scientific
>institutions, private sector etc.). This second workshop
>aims to offer to scientists with advanced expertise in risk
>assessment and/or biosafety regulative framework a dedicated
>forum for advanced discussion and information sharing
>
> The general approach in both workshops will focus, with
>special emphasis, on case study presentations and discussion
>for the environmental release of genetically modified
>organisms (GMOs). Detailed programs will be distributed when
>available.
>
>Preference will be given to applicants from developing
>countries. A limited number of grants will be available for
>scientists from ICGEB Member Countries. These grants will
>cover accommodation meals and incidentals for the duration
>of the course; travel is NOT funded. Registration for the
>Workshop is limited to 30 participants.
>
>Note . ICGEB is looking for Sponsors / Co-sponsors /
>Contributors in order to recognize contributions toward
>travel expenses of the participants. Interested
>organizations may wish to contact the ICGEB
>Secretariat in due time to facilitate the widest
>participation to both workshops.
>____________________________________________________
>
>For more information on ICGEB biosafety activities, please
>contact:
>
>Giovanni Ferraiolo - mailto:biosafe@icgeb.trieste.it
>ICGEB Biosafety, Programme Officer,
>
>International Centre for Genetic Engineering and
>Biotechnology. Area Science Park, Padriciano 99 I-34012
>Trieste (Italy). Tel: +39 040 3757364 - Fax: +39 040 226555
>
>ICGEB Biosafety WebPages:
>____________________________________________________
>
>The International Centre for Genetic Engineering and
>Biotechnology (ICGEB) is an autonomous, international,
>intergovernmental organization formed by 61 signatory
>countries of which 43 are full Member States. The Centre is
>dedicated to advanced research and training in molecular
>biology and biotechnology. The mandate of the ICGEB is to
>promote the safe use of biotechnology world-wide and with
>special regard to the needs of the developing world. Further
>information on the ICGEB could be found on its web pages at
>
>____________________________________________________
>
>mlt2
>unsubscribe
=========================================================================
Date: Tue, 24 Aug 1999 16:16:22 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: Good Laboratory Practice
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
"No food and drinks in the laboratory" is a common practice when one is
working in the laboratory. Is this a strict regulation that should apply to
some food laboratory? There will be some food control tests on taste and
smell. What are the safety regulation on this laboratory?
YK Wan
Safety Officer
Please reply when you receive the message. Thank you.
***** Yu Kwan WAN,
***** Safety Officer
***** The Chinese University of Hong Kong
***** Shatin, NT, Hong Kong
***** Email:
***** ulsoykwan@cuhk.edu.hk
***** ulsoykwan@
***** ulsoykwan@
***** ulsoykwan@
=========================================================================
Date: Tue, 24 Aug 1999 07:21:40 EDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: Digest Formats
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Hi Biosafety,
When I receive the DIGEST Format, it's in the "mim" form. I can't read this.
How do I get it in the "txt" form? ... Jim
=========================================================================
Date: Tue, 24 Aug 1999 08:24:55 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Exposure control vs Biosafety Manual
MIME-Version: 1.0
Content-Type: text/plain
Exposure Control Manual vs Biosafety Manual
How many of you have an Exposure Control Document that all employees are
subjected to read and comply with its written policy?
Compared to a
How many of you have a Biosafety Manual that employees or staff must read
and comply with its written rules and policies if they have the potential to
come in contact with a biohazard?
or
How many of you have both?
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Tue, 24 Aug 1999 08:46:41 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Don Callihan
Subject: Re: Exposure control vs Biosafety Manual
Mime-Version: 1.0
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(Embedded Don Callihan @ BDX
image moved 08/24/99 08:46 AM
to file: (Embedded image moved to file: pic22508.pcx)
pic15725.pcx)
You must have both. We have a separate policy for our exposure control plan that
is included in our biosafety manual. The policy is our compliance document and
the biosafety manual has additional material to help people understand both the
BBP standard and the nature of the specific agents like HIV, HBV, HCV, etc. This
was also the way I organized the material when I was manager of a clinical
microbiology lab. Hope this helps.
Don Callihan, Ph.D., DABMM
Senior Clinical Microbiologist and Biosafety Officer
BD Biosciences
P.O. Box 999 - MC982
Sparks, MD 21152-0999
410.773.6684
Schlank Bliss BM on 08/24/99 08:24:55 AM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Don Callihan/BALT/BDX)
Subject: Exposure control vs Biosafety Manual
Exposure Control Manual vs Biosafety Manual
How many of you have an Exposure Control Document that all employees are
subjected to read and comply with its written policy?
Compared to a
How many of you have a Biosafety Manual that employees or staff must read
and comply with its written rules and policies if they have the potential to
come in contact with a biohazard?
or
How many of you have both?
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
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=========================================================================
Date: Tue, 24 Aug 1999 09:30:13 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Exposure control vs Biosafety Manual
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Our approach has been to include the biosafety information as a chapter of
our laboratory safety manual. We have recently split this off as a
separate document. All labs and all new personnel will shortly begin
recieving a computer disk that contains the safety manuals in a .pdf
format. Each lab is required to develope it's own separate exposure
control plan for it's lab using the CWRU safety manuals.
The biosafety manual contains University Information and policies.
The exposure control plan is for the lab only.
Bob
>Exposure Control Manual vs Biosafety Manual
>
>How many of you have an Exposure Control Document that all employees are
>subjected to read and comply with its written policy?
>
>Compared to a
>
>How many of you have a Biosafety Manual that employees or staff must read
>and comply with its written rules and policies if they have the potential to
>come in contact with a biohazard?
>
>or
>
>How many of you have both?
>
>
>Bliss M. Schlank
>Biosafety Specialist
>AstraZeneca
>1800 Concord Pike
>Wilmington DE 19850-5437
>302.886.2185 Fax: 302.886.2909
>bliss.schlank@phwilm.
>
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Tue, 24 Aug 1999 09:08:35 -0500
Reply-To: "mkinsey@"
Sender: A Biosafety Discussion List
From: Melina Kinsey
Organization: MRI
Subject: Re: Exposure control vs Biosafety Manual
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
We have a Biological Safety Plan (Biosafety Manual) that all employees who work
in the Biotech section must ready and comply with. I would be interested in
reviewing an Exposure Control Document, if anyone uses one and would be willing
to send me a copy.
Melina Kinsey
Biosafety Officer
Midwest Research Institute
425 Volker Blvd.
Kansas City, MO 64110
(816) 753-7600 x1424
mkinsey@
=========================================================================
Date: Tue, 24 Aug 1999 09:25:04 -0500
Reply-To: "mkinsey@"
Sender: A Biosafety Discussion List
From: Melina Kinsey
Organization: MRI
Subject: OOPS! RE: Exposure control vs Biosafety Manual
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
DUH!!!
My boss just explained to me that an Exposure Control Plan is another name for
the BBP Plan. We have this covered in our Chemical Hygiene Plan that is given
to all our employees and our Biological Safety Plan refers to this document.
So I guess my correct response is yes we have both. Sorry if I confused
others on the list.
Melina Kinsey
Biosafety Officer
Midwest Research Institute
425 Volker Blvd.
Kansas City, MO 64110
(816) 753-7600 x1424
mkinsey@
-----Original Message-----
From: Kinsey, Melina
Sent: Tuesday, August 24, 1999 9:09 AM
To: 'A Biosafety Discussion List'
Subject: RE: Exposure control vs Biosafety Manual
We have a Biological Safety Plan (Biosafety Manual) that all employees who work
in the Biotech section must ready and comply with. I would be interested in
reviewing an Exposure Control Document, if anyone uses one and would be willing
to send me a copy.
Melina Kinsey
Biosafety Officer
Midwest Research Institute
425 Volker Blvd.
Kansas City, MO 64110
(816) 753-7600 x1424
mkinsey@
=========================================================================
Date: Tue, 24 Aug 1999 08:01:43 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Dan Shawler
Subject: Re: Exposure control vs Biosafety Manual
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
We have both. The BBP Exposure Control Plan is the document that defines
our institution's policy and which complies with the California BBP
standards (which are slightly different from the federal standards). The
Biosafety Manual is given to all laboratory employees and is a translation
of the ECP into plain english. It emphasizes the standard operating
procedures that comply with the ECP rather than just stating what the rules
and regulations are.
Dan Shawler
dshawler@
=========================================================================
Date: Tue, 24 Aug 1999 10:05:45 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Clifford W. Bond"
Subject: unsubscribe
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Clifford W. Bond, Professor
Department of Microbiology
Montana State University
Bozeman, MT 59717-3520
Email: umbcb@gemini.oscs.montana.edu
Internet:
Telephone: 406 994-4130
TeleFAX: 406 994-4926
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Giovanni Ferraiolo ICGEB BIOSAFETY
Sent: Monday, August 23, 1999 8:06 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: *** ICGEB BIOSAFETY NEWS - AUGUST 1999 ***
____________________________________________________
*** ICGEB BIOSAFETY NEWS - AUGUST 1999 ***
____________________________________________________
This message is to keep you updated with the news concerning
the ICGEB Biosafety WebPages and the activity of the Centre
in this issue. If you DO NOT want to receive further
messages, just REPLY typing "unsubscribe" in the "Subject"
field and your name will be automatically deleted from the
mailing list. ICGEB apologizes for any inconvenience due to
undesired posting.
____________________________________________________
TWO BIOSAFETY WORKSHOPS IN THE ICGEB YEAR 2000 CALENDAR
Two different workshops on biosafety and risk assessment for
the environmental release of genetically modified organisms
(GMOs) have been scheduled within the Year 2000 ICGEB
Calendar of Activities:
*** 27-31 March 2000, Trieste, ITALY. Workshop:
"Biosafety 1. Science and Policy in Risk Assessment of
Transgenic Organisms: A Case Study Approach".
Organizers: George Tzotzos, UNIDO, Vienna, Austria; Gilbert
Howe, University of Bristol, UK; Giovanni Ferraiolo, ICGEB,
Trieste, Italy.
*** 3-8 April 2000, Florence, ITALY. Workshop: "Biosafety
2. Advanced Research and Procedures: Case Studies for
Designated Experts".
Organizers: Marcello Broggio, Istituto Agronomico per
l'Oltremare, Florence, Italy; Giovanni Ferraiolo, ICGEB,
Trieste, Italy.
Requests for information and applications directly to:
Ms. Micaela Di Blas, ICGEB, Padriciano 99, 34012 Trieste,
Italy. Tel: +39-040-3757333; Fax: +39-040-226555;
mailto:courses@icgeb.trieste.it
* Biosafety 1: The workshop aims to offer a comprehensive
approach to biosafety and is directed to scientists
actively involved in biological risk assessment and/or
biotechnology regulations. ICGEB is dealing with biosafety
since its inception and since 1991 the Centre organizes
annual workshops on this issue attended, to date, from over
500 scientists involved in biosafety issues in more than 50
different countries.
* Biosafety 2: Following the suggestions of many biosafety
focal-points and resource persons we have added, in our
agenda for the year 2000, an advanced workshop, directed
exclusively to officers in Governmental Agencies and/or
designated experts, working in the area of risk assessment
of GMOs at official level (governments, scientific
institutions, private sector etc.). This second workshop
aims to offer to scientists with advanced expertise in risk
assessment and/or biosafety regulative framework a dedicated
forum for advanced discussion and information sharing
The general approach in both workshops will focus, with
special emphasis, on case study presentations and discussion
for the environmental release of genetically modified
organisms (GMOs). Detailed programs will be distributed when
available.
Preference will be given to applicants from developing
countries. A limited number of grants will be available for
scientists from ICGEB Member Countries. These grants will
cover accommodation meals and incidentals for the duration
of the course; travel is NOT funded. Registration for the
Workshop is limited to 30 participants.
Note . ICGEB is looking for Sponsors / Co-sponsors /
Contributors in order to recognize contributions toward
travel expenses of the participants. Interested
organizations may wish to contact the ICGEB
Secretariat in due time to facilitate the widest
participation to both workshops.
____________________________________________________
For more information on ICGEB biosafety activities, please
contact:
Giovanni Ferraiolo - mailto:biosafe@icgeb.trieste.it
ICGEB Biosafety, Programme Officer,
International Centre for Genetic Engineering and
Biotechnology. Area Science Park, Padriciano 99 I-34012
Trieste (Italy). Tel: +39 040 3757364 - Fax: +39 040 226555
ICGEB Biosafety WebPages:
____________________________________________________
The International Centre for Genetic Engineering and
Biotechnology (ICGEB) is an autonomous, international,
intergovernmental organization formed by 61 signatory
countries of which 43 are full Member States. The Centre is
dedicated to advanced research and training in molecular
biology and biotechnology. The mandate of the ICGEB is to
promote the safe use of biotechnology world-wide and with
special regard to the needs of the developing world. Further
information on the ICGEB could be found on its web pages at
____________________________________________________
mlt2
=========================================================================
Date: Tue, 24 Aug 1999 10:06:20 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Clifford W. Bond"
Subject: unsubscribe
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
Clifford W. Bond, Professor
Department of Microbiology
Montana State University
Bozeman, MT 59717-3520
Email: umbcb@gemini.oscs.montana.edu
Internet:
Telephone: 406 994-4130
TeleFAX: 406 994-4926
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of EITAN
Sent: Monday, August 23, 1999 11:07 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: *** ICGEB BIOSAFETY NEWS - AUGUST 1999 ***
-----Original Message-----
From: Giovanni Ferraiolo ICGEB BIOSAFETY
To: BIOSAFTY@MITVMA.MIT.EDU
Date: =E9=E5=ED =F9=F0=E9 23 =E0=E5=E2=E5=F1=E8 1999 17:20
Subject: *** ICGEB BIOSAFETY NEWS - AUGUST 1999 ***
>____________________________________________________
>
> *** ICGEB BIOSAFETY NEWS - AUGUST 1999 ***
>
>____________________________________________________
>
>This message is to keep you updated with the news concerning
>the ICGEB Biosafety WebPages and the activity of the Centre
>in this issue. If you DO NOT want to receive further
>messages, just REPLY typing "unsubscribe" in the "Subject"
>field and your name will be automatically deleted from the
>mailing list. ICGEB apologizes for any inconvenience due to
>undesired posting.
>____________________________________________________
>
>TWO BIOSAFETY WORKSHOPS IN THE ICGEB YEAR 2000 CALENDAR
>
>Two different workshops on biosafety and risk assessment for
>the environmental release of genetically modified organisms
>(GMOs) have been scheduled within the Year 2000 ICGEB
>Calendar of Activities:
>
> *** 27-31 March 2000, Trieste, ITALY. Workshop:
>"Biosafety 1. Science and Policy in Risk Assessment of
>Transgenic Organisms: A Case Study Approach".
>Organizers: George Tzotzos, UNIDO, Vienna, Austria; Gilbert
>Howe, University of Bristol, UK; Giovanni Ferraiolo, ICGEB,
>Trieste, Italy.
>
> *** 3-8 April 2000, Florence, ITALY. Workshop: "Biosafety
>2. Advanced Research and Procedures: Case Studies for
>Designated Experts".
>Organizers: Marcello Broggio, Istituto Agronomico per
>l'Oltremare, Florence, Italy; Giovanni Ferraiolo, ICGEB,
>Trieste, Italy.
>
> Requests for information and applications directly to:
>Ms. Micaela Di Blas, ICGEB, Padriciano 99, 34012 Trieste,
>Italy. Tel: +39-040-3757333; Fax: +39-040-226555;
>mailto:courses@icgeb.trieste.it
>
>
>
>* Biosafety 1: The workshop aims to offer a comprehensive
>approach to biosafety and is directed to scientists
>actively involved in biological risk assessment and/or
>biotechnology regulations. ICGEB is dealing with biosafety
>since its inception and since 1991 the Centre organizes
>annual workshops on this issue attended, to date, from over
>500 scientists involved in biosafety issues in more than 50
>different countries.
>
>* Biosafety 2: Following the suggestions of many biosafety
>focal-points and resource persons we have added, in our
>agenda for the year 2000, an advanced workshop, directed
>exclusively to officers in Governmental Agencies and/or
>designated experts, working in the area of risk assessment
>of GMOs at official level (governments, scientific
>institutions, private sector etc.). This second workshop
>aims to offer to scientists with advanced expertise in risk
>assessment and/or biosafety regulative framework a dedicated
>forum for advanced discussion and information sharing
>
> The general approach in both workshops will focus, with
>special emphasis, on case study presentations and discussion
>for the environmental release of genetically modified
>organisms (GMOs). Detailed programs will be distributed when
>available.
>
>Preference will be given to applicants from developing
>countries. A limited number of grants will be available for
>scientists from ICGEB Member Countries. These grants will
>cover accommodation meals and incidentals for the duration
>of the course; travel is NOT funded. Registration for the
>Workshop is limited to 30 participants.
>
>Note . ICGEB is looking for Sponsors / Co-sponsors /
>Contributors in order to recognize contributions toward
>travel expenses of the participants. Interested
>organizations may wish to contact the ICGEB
>Secretariat in due time to facilitate the widest
>participation to both workshops.
>____________________________________________________
>
>For more information on ICGEB biosafety activities, please
>contact:
>
>Giovanni Ferraiolo - mailto:biosafe@icgeb.trieste.it
>ICGEB Biosafety, Programme Officer,
>
>International Centre for Genetic Engineering and
>Biotechnology. Area Science Park, Padriciano 99 I-34012
>Trieste (Italy). Tel: +39 040 3757364 - Fax: +39 040 226555
>
>ICGEB Biosafety WebPages:
>____________________________________________________
>
>The International Centre for Genetic Engineering and
>Biotechnology (ICGEB) is an autonomous, international,
>intergovernmental organization formed by 61 signatory
>countries of which 43 are full Member States. The Centre is
>dedicated to advanced research and training in molecular
>biology and biotechnology. The mandate of the ICGEB is to
>promote the safe use of biotechnology world-wide and with
>special regard to the needs of the developing world. Further
>information on the ICGEB could be found on its web pages at
>
>____________________________________________________
>
>mlt2
>unsubscribe
=========================================================================
Date: Tue, 24 Aug 1999 19:24:16 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Andrew Cockburn
Subject: NFS 49 or not?
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Hi all,
Before my arrival, different departments used different vendors to certify
their biosafety cabinets. Most vendors certified to NSF-49, but at least one
used "manufacturer's specifications", whatever that means.
This year I talked to an experienced vendor who certifies to NSF-49 and
negotiated a good price ($110 each) based on a volume discount. However one
department wants to use a different vendor (not a cost issue, they just want
to be different). This has the potential to raise overall costs to WVU
because of the loss of volume discounts.
How do other universities handle this: does the IBO choose a vendor and then
everyone has to use that vendor? What about not certifying to NSF-49 or
using personnel that are not NSF accredited?
How would you handle this: (1) let everyone use any vendor, (2) provide a
short list of acceptable vendors, or (3) negotiate a blanket order with one
vendor and tell everyone that they have to use it?
Andrew Cockburn, PhD
Director of Biological Safety
West Virginia University
Morgantown, WV 26506-9006
Telephone: 304-293-7157
FAX: 304-293-4529
Email: acockbur@wvu.edu
=========================================================================
Date: Tue, 24 Aug 1999 19:33:50 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Andrew Cockburn
Subject: NFS 49 or not?
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Hi all,
Before my arrival, different departments used different vendors to certify
their biosafety cabinets. Most vendors certified to NSF-49, but at least one
used "manufacturer's specifications", whatever that means.
This year I talked to an experienced vendor who certifies to NSF-49 and
negotiated a good price ($110 each) based on a volume discount. However one
department wants to use a different vendor (not a cost issue, they just want
to be different). This has the potential to raise overall costs to WVU
because of the loss of volume discounts.
How do other universities handle this: does the IBO choose a vendor and then
everyone has to use that vendor? What about not certifying to NSF-49 or
using personnel that are not NSF accredited?
How would you handle this: (1) let everyone use any vendor, (2) provide a
short list of acceptable vendors, or (3) negotiate a blanket order with one
vendor and tell everyone that they have to use it?
(Sorry if this is a duplicate; I got an error message the first time I sent
it.)
Andrew Cockburn, PhD
Director of Biological Safety
West Virginia University
Morgantown, WV 26506-9006
Telephone: 304-293-7157
FAX: 304-293-4529
Email: acockbur@wvu.edu
=========================================================================
Date: Wed, 25 Aug 1999 09:00:55 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: NFS 49 or not?
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Our department negociated a contract for the University. Any lab that
needs recertification of a BSC simply calls us to schedule a time.
Bob
>Hi all,
>
>Before my arrival, different departments used different vendors to certify
>their biosafety cabinets. Most vendors certified to NSF-49, but at least one
>used "manufacturer's specifications", whatever that means.
>
>This year I talked to an experienced vendor who certifies to NSF-49 and
>negotiated a good price ($110 each) based on a volume discount. However one
>department wants to use a different vendor (not a cost issue, they just want
>to be different). This has the potential to raise overall costs to WVU
>because of the loss of volume discounts.
>
>How do other universities handle this: does the IBO choose a vendor and then
>everyone has to use that vendor? What about not certifying to NSF-49 or
>using personnel that are not NSF accredited?
>
>How would you handle this: (1) let everyone use any vendor, (2) provide a
>short list of acceptable vendors, or (3) negotiate a blanket order with one
>vendor and tell everyone that they have to use it?
>
>Andrew Cockburn, PhD
>Director of Biological Safety
>West Virginia University
>Morgantown, WV 26506-9006
>
>Telephone: 304-293-7157
>FAX: 304-293-4529
>Email: acockbur@wvu.edu
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Wed, 25 Aug 1999 08:06:50 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Kyle Boyett
Subject: Re: NFS 49 or not?
MIME-Version: 1.0
Content-Type: text/plain; charset="ISO-8859-1"
Andrew, We have enough BSC's to justify a couple of people in our department
that do all the certifications. Depending on the number that you have you
may be able to save money by doing the certifications in-house. But as I
think about it, you may want to address why this particular department
chooses to use someone other than the contract vendor. We had a similar
problem about 10 years ago. Most of the problem stemmed from credibility of
the certifier that was used. Once that was re-established we have not had
any problems since. Let me know if I can be of further help.
Kyle Boyett
Asst. Director of Biosafety
Occupational Health and Safety
University of Alabama at Birmingham
e-mail- kboyett@healthsafe.uab.edu
Phone- 205-934-2487
** Asking me to overlook a safety violation is like asking me to reduce the
value I place on YOUR life**
-----Original Message-----
From: Andrew Cockburn [mailto:acockbur@WVU.EDU]
Sent: Tuesday, August 24, 1999 6:24 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: NFS 49 or not?
Hi all,
Before my arrival, different departments used different vendors to certify
their biosafety cabinets. Most vendors certified to NSF-49, but at least one
used "manufacturer's specifications", whatever that means.
This year I talked to an experienced vendor who certifies to NSF-49 and
negotiated a good price ($110 each) based on a volume discount. However one
department wants to use a different vendor (not a cost issue, they just want
to be different). This has the potential to raise overall costs to WVU
because of the loss of volume discounts.
How do other universities handle this: does the IBO choose a vendor and then
everyone has to use that vendor? What about not certifying to NSF-49 or
using personnel that are not NSF accredited?
How would you handle this: (1) let everyone use any vendor, (2) provide a
short list of acceptable vendors, or (3) negotiate a blanket order with one
vendor and tell everyone that they have to use it?
Andrew Cockburn, PhD
Director of Biological Safety
West Virginia University
Morgantown, WV 26506-9006
Telephone: 304-293-7157
FAX: 304-293-4529
Email: acockbur@wvu.edu
=========================================================================
Date: Wed, 25 Aug 1999 09:43:58 EDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jennifer Minogue
Organization: Environmental Health and Safety
Subject: Re: NFS 49 or not?
MIME-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
We use only NFS certified people. We currently have two vendors who do
the testing. This keeps any one vendor from becoming too
complacent and keeps the prices in line.
EHS office pays for the annual testing. This way we know that
it gets done and we keep the records (due diligence). If the
cabinet requires repairs OR needs to be moved, then the
originating department/researcher must pay the costs.
Jennifer E. Minogue, B.S., M.S.P.H.
Hazardous Materials Safety Officer
Environmental Health and Safety
University of Guelph
Guelph, Ontario N1G 2W1 Canada
519-824-4120 X3190 Fax 519-824-0364
e-Mail jennifer@ehs.uoguelph.ca
=========================================================================
Date: Wed, 25 Aug 1999 09:54:58 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: ABSA conf. program
Mime-Version: 1.0
Content-Type: multipart/mixed; boundary="=====================_935603698==_"
--=====================_935603698==_
Content-Type: text/plain; charset="us-ascii"
Attached is the ABSA conf. program. I have attached it as 1) a Word 97
document and 2) as a text document.
It should be up on the web site within 48 hours.
--=====================_935603698==_
Content-Type: text/plain; charset="us-ascii"
AMERICAN BIOLOGICAL
SAFETY ASSOCIATION
15TH ANNIVERSARY
42nd Annual Biological
Safety Conference
Regal Riverfront, St. Louis
October 17-20, 1999
PRELIMINARY PROGRAM
PRELIMINARY PROGRAM
OCTOBER 17-20, 1999
Regal Riverfront, St. Louis, Missouri
FRIDAY, OCTOBER 15, 1999
PRE-CONFERENCE COURSES
1:00 - 5:00
1. REVIEW COURSE FOR BIOSAFETY AND NATIONAL REGISTRY OF MICROBIOLOGISTS (NRM) EXAM IN BIOLOGICAL SAFETY MICROBIOLOGY
Mary Cipriano, RBP, CBSP and Marian Downing, CBSP, Abbott Laboratories, Abbott Park, IL; Benjamin Fontes, CBSP, Yale University, New Haven, CT; Glenn Funk, CBSP, Univ of CA, San Francisco, CA; Christine Thompson, RBP, CBSP, Eli Lilly, Indianapolis, IN
1:00 - 5:00
2. BIOSAFETY LEVEL 3 (BSL3) PROGRAMS: IN LABORATORIES, ANIMAL FACILITIES, AND THE FIELD
Karen Byers, RBP, CBSP, Dana-Farber Cancer Institute, Boston, MA; Benjamin Fontes, CBSP, Yale University, New Haven, CT; Linda Wolfe, RBP, CBSP, Whitehead Institute, Cambridge, MA
SATURDAY, OCTOBER 16, 1999
7:00 - 5:00 REGISTRATION
PRE-CONFERENCE COURSES
8:00 - 5:00 - Continuation
1. REVIEW COURSE FOR BIOSAFETY AND NATIONAL REGISTRY OF MICROBIOLOGISTS (NRM) EXAM IN BIOLOGICAL SAFETY MICROBIOLOGY
Mary Cipriano, RBP, CBSP, Marian Downing, CBSP, Benjamin Fontes, CBSP, Glenn Funk, CBSP, Christine Thompson, RBP, CBSP
8:00 - 5:00
3. RISK ASSESSMENT
Lynn Harding, CBSP, Biosafety Consultant, Chattanooga, TN; Diane O. Fleming, RBP, CBSP, Biosafety Consultant, Bowie, MD
8:00 - 5:00
4. BIOSAFETY LEVEL 3 (BSL3) DESIGN AND COMMISSIONING
Esmeralda Prat-Party, RBP, Rockefeller University, New York , NY; Lee Alderman, Emory University, Atlanta, GA; Janet S. Baum, Health Education & Research Associates
8:00 - 5:00
5. THE SCIENCE, TECHNIQUES, AND JARGON OF MOLECULAR BIOLOGY
Patrick Condreay, Research Investigator, Department of Molecular Sciences, Glaxo Wellcome R&D
8:00 - 12:00
6. VENTILATION ISSUES FOR BIOSAFETY PROFESSIONALS
Byron S. Tepper, CBSP, Biocontrol, Inc. Lutherville, MD; Jerrold Koenigsberg, GPR/Sverdrup Corporation
1:00 - 5:00
7. BIOAEROSOLS
Jerry J. Tulis, Duke University Medical Center, Durham, NC
SUNDAY, OCTOBER 17, 1999
7:00 - 5:00 REGISTRATION
PRE-CONFERENCE COURSES
8:00 - 5:00 - Continuation
1. REVIEW COURSE FOR BIOSAFETY AND NATIONAL REGISTRY OF MICROBIOLOGISTS (NRM) EXAM IN BIOLOGICAL SAFETY MICROBIOLOGY
Mary Cipriano, RBP, CBSP, Marian Downing, CBSP, Benjamin Fontes, CBSP, Glenn Funk, CBSP, Christine Thompson, RBP, CBSP
8:00 - 5:00
8. INDUSTRIAL HYGIENE FOR BIOSAFETY PROFESSIONALS
Matthew Finucane, University of Pennsylvania, Philadelphia, PA; Larry Gibbs, Stanford University, Stanford, CA
8:00 - 12:00
9. ADVANCED DISINFECTION
Richard Fink, CBSP, Massachusetts Inst. of Technology, Cambridge, MA; Syed Sattar, University of Ottawa, Canada; Donald Vesley, CBSP, University of Minnesota, Minneapolis, MN
1:00 - 5:00
10. MEDICAL SURVEILLANCE
Gregg Kasting, Medical Director, CDC Occupational Health Clinic DynCorp, Atlanta, GA
6:00 - 8:00 OPENING RECEPTION - EXHIBIT HALL
REGAL RIVERFRONT HOTEL
MONDAY, OCTOBER 18, 1999
7:00 - 5:00 REGISTRATION
8:30 - 8:40 LAC Welcome
8:40 - 8:45 SPC Welcome
8:45 - 9:15 PRESIDENT'S ADDRESS
Marilyn Misenheimer, RBP, CBSP, President, ABSA
University of Southern California
9:15 - 9:30 EVERETT J. HANEL, JR. AWARD PRESENTATION
9:30 - 10:30 SESSION 1 - EAGLESON LECTURE
Ronald Crystal, New York Weill Cornell Center, New York, NY
10:30 - 10:50 COFFEE BREAK & EXHIBITS
10:50 - 12:10 SESSION 2 - DISINFECTION & EXPOSURE
Moderator: Bob Hawley, RBP, CBSP
10:50 - 11:10
EFFICACY OF DISINFECTANTS AGAINST MYCOBACTERIA SPP. INCLUDING DRUG RESISTANT STRAINS
Maureen Best, Office of Biosafety, Health Canada, Ottawa, Canada; Syed A. Sattar, Centre for Research on Environmental Microbiology, Ottawa, Canada
11:10 - 11:30
CHEMICAL GERMICIDES AND RECENT DEVELOPMENTS IN THE ASSESSMENT OF THEIR ACTIVITY
Syed A. Sattar, Centre for Research on Environmental Microbiology; Susan Springthorpe, Faculty of Medicine, University of Ottawa, Ontario, Canada
11:30 - 11:50
ASSESSING THE LEVEL OF REPORTING OF PERCUTANEOUS INJURIES
Debra L. Hunt, RBP, CBSP and Karen Worthington, Duke University Medical Center, Durham, NC
11:50 - 12:10
STERILIZATION WITH GASEOUS CHLORINE DIOXIDE
Diane L. Battisti, Johnson & Johnson, New Brunswick, NJ
12:10 - 1:10 LUNCH & EXHIBITS
1:10 - 2:40 SESSION 3 - TRAINING
Moderator: Bob Hashimoto
1:10 - 1:40
LEARNING TO TEACH LABORATORY SAFETY ON THE WEB
Emmett Barkley, Howard Hughes Medical Institute, Chevy Chase, MD
1:40 - 2:00
UPDATE OF WEB BASED INTERNET BLOODBORNE PATHOGENS TRAINING PROGRAM FOR RESEARCH AND CLINICAL LAB EMPLOYEES
Ben Fontes, CBSP; Maryjo Lanzillotta; Tom Ouimet; and Phil Simon, Yale University, New Haven, CT
2:00 - 2:20
COMMENTS ON THE 4TH EDITION OF THE BMBL
Jonathan Y. Richmond, RBP, Office of Health and Safety, CDC, Atlanta, GA
2:20 - 2:40
STANFORD'S EXPERIENCE WITH VARIOUS TRAINING METHODS
Larry Gibbs, Environmental Health & Safety, Stanford University, Stanford, CA
2:40 - 3:00 COFFEE BREAK & EXHIBITS
3:00 -5:00 BUSINESS MEETING
TUESDAY, OCTOBER 19, 1999
7:00 - 5:00 REGISTRATION
7:00 - 4:00 EXHIBIT HALL
7:00 - 8:00 CONTINENTAL BREAKFAST / EXHIBITS
8:00 - 4:00 TRAINING EXHIBITS
10:00 - 5:00 POSTERS DISPLAYED
8:30 - 10:30 SESSION 4
- INFLUENZA, AIRBORNE ORGANISMS
Moderator: Rosamond Rutledge-Burns, CBSP
8:30 - 9:30 WEDUM MEMORIAL LECTURE
SEARCHING FOR THE SECRETS OF SPANISH FLU IN SVALBARD, NORWAY
Kirsty Duncan, University of Windsor, Ontario, Canada
9:30 - 10:00
HONG KONG FLU - BIOSAFETY ISSUES OF MANUFACTURE OF THE EXPERIMENTAL VACCINE
Heather J. Sheeley, Porton Down, Salisbury, Wiltshire, England
10:00 - 10:30 GROSS AWARD LECTURE
INFLUENCE OF RELATIVE HUMIDITY ON PARTICLE SIZE AND UV SENSITIVITY OF SERRATIA MARCESCENS AND BCGT AEROSOLS
Gwangpyo Ko, Melvin W. First, and Harriet A. Burge, Department of Environmental Health, Harvard School of Public Health, Boston, MA
10:30 - 10:50 COFFEE BREAK & EXHIBITS
10:50 - 12:10 PLENARY SESSION 5
INTERNATIONAL ISSUES
Moderator: Joseph Kozlovac, RBP
10:50 - 11:10
BIOLOGICAL SAFETY FROM A GLOBAL PERSPECTIVE
Halil Gueltekin, Abbott GmbH., Wiesbaden, Germany; Penny H. Holeman, RBP, CBSP, Johnson & Johnson, Raritan, NJ
11:10 - 11:30
PRACTICAL EXPERIENCES USING A NOVEL WASTE DISPOSAL SYSTEM IN AN R & D INDUSTRIAL ENVIRONMENT IN THE US AND FRANCE
Francine E. Preston, SmithKline Beecham Pharmaceuticals, R & D, Collegeville, PA
11:30 - 11:50
ACHIEVING QUALITY BIOCONTAINMENT IN CERTAIN LABORATORIES OUTSIDE THE US
Jonathan Y. Richmond, RBP, Office of Health and Safety, CDC, Atlanta, GA
11:50 - 12:10
GENETIC MODIFICATION IN EUROPE - PERCEPTIONS & CONTROLS
John R. Keddie, SmithKline Beecham Pharmaceuticals, Harlow, Essex, UK
12:10 - 1:30 LUNCH AND EXHIBITS
1:30 - 2:40 BIOTERRORISM ROUNDTABLE
Stefan Wagener, RBP, CBSP, Michigan State University, Lansing MI; Robert Hawley, USAMRIID; Barbara Johnson, US Army; Representative from CDC
2:40 - 3:20 BREAK/POSTERS
3:20 - 5:00 CONCURRENT SESSION 6 - FACILITY DESIGN
Moderator: Phil Hauck, RBP
3:20 - 3:40
A STATUS REPORT ON CDC'S NEWEST CONTAINMENT LABORATORY BUILDING
William Howard, Office of Health and Safety, CDC, Atlanta, GA
3:40 - 3:55
VALIDATION OF A NECROPSY TABLE FOR AEROSOL BIOCONTAINMENT
Allan Bennett and Simon Parks, Center for Applied Microbiology & Research, Porton Down, UK
3:55 - 4:15
ASSESSMENT OF CONTAINMENT AND PRODUCT PROTECTION IN A CLASS II TYPE B3 BIOLOGICAL: SAFETY CABINET UNDER AS-USED LABORATORY CONDITIONS
Peter Harris & Dave Lupo, B & V Testing, Inc., Waltham, MA
4:15 - 4:40
DESIGN AND VENTILATION SYSTEMS FOR LEVEL 3 LABORATORIES
Allan Bennett, Simon Parks, & John Benbough, Centre for Applied Microbiology and Research, Salisbury, UK
4:40 - 5:00
FACILITY GUIDELINE MODIFICATIONS IN THE 4TH EDITION OF BIOSAFETY IN MICROBIOLOGICAL AND BIOMEDICAL LABORATORIES
Jonathan T. Crane, AIA, CUH2A Inc., Atlanta, GA
3:20 - 5:00 CONCURRENT SESSION 7
HAZARDOUS WASTE
Moderator: Curtis Speaker
3:20 - 3:40
THE USE OF THE TEMPICO(r) ROTOCLAVE IN THE TREATMENT OF PATHOLOGICAL WASTE
Larry J. Thompson, Cornell University, Ithaca, NY; Ira F. Salkin, New York State Department of Health; New York, NY
3:40 - 4:00
EVALUATING AND SELECTING THE BEST BIOMEDICAL WASTE TREATMENT AND DISPOSAL ALTERATIVE IN CONSIDERATION OF RECENT STATE AND FEDERAL REGULATIONS
Lawrence G. Doucet, Doucet & Mainka, PC, Peekskill, NY
4:00 - 4:20
CONTAMINATED PRODUCT RETURN: A SAFE, SIMPLE AND COMPLIANT TRANSPORTATION SOLUTION
Penny H. Holeman, RBP. CBSP, Johnson & Johnson, Raritan, NJ; Beth Adams, Johnson & Johnson Health Care Systems, Inc., Piscataway, NJ
4:20 - 4:40
INSPECTIONS OF INFECTIOUS SUBSTANCES SHIPMENTS
Bill Homovec, CBSP, Laboratory Corporation of America Holdings, Burlington, NC
POSTER PRESENTATIONS
ERGONOMICS AND THE BIOLOGICAL RESEARCHER
Janel E. Mello, Biology and Biotechnology research program, Lawrence Livermore, National Laboratory, University of California, Livermore, CA
A SIMPLE APPARATUS TO REDUCE THE INCIDENCE OF AUTO-INOCULATION DURING INTRAVENOUS RODENT TAIL VEIN INJECTION
Michelle S. DeStefano, SUNY Health Science Center, Syracuse, NY; Michael H. Cynamon, SUNY Health Science Center, Syracuse, NY
AERODYNAMIC BEHAVIOR OF BIOAEROSOLS: DIFFERENT COLLECTION PATTERN FROM NATURAL OR CHEMICAL AEROSOLS BY THE MIDDLE EFFICIENCY AIR FILTER
Katsuaki Shinohara1, Hiroshi Hamasaki 2; Fumio Kanoh3, Masayuki Uezono2, Yukio Innami2, Noriyuki Tokura2, Mikio Suzuki2, Atsuo Kitabayashi2, Hiroki Fujii1, Kazuyoshi Sugiyama1; 1Division of Biosafety Control and Research, National Institute of Infectious Diseases, Tokyo, Japan; 2 Hitachi Air Conditioning and Refrigeration Co, Ltd, Tokyo, Japan; 3Tokyo Metropolitan Research Laboratory of Public Health, Tokyo, Japan.
IMPLEMENTATION AND DEVELOPMENT OF A UNIVERSITY'S INFECTION CONTROL PROGRAM
Maryjo C. Lanzillotta and Ben Fontes, CBSP, Yale University, New Haven, CT
AN EFFECTIVE PROGRAM FOR HAZARDOUS AGENT IDENTIFICATION AND RISK ASSESSMENT IN ANIMAL BIOMEDICAL RESEARCH AT A LARGE AND DIVERSE INSTITUTION
Randina Prete and Ben Fontes, CBSP, Yale University, New Haven, CT
THE TRUE STABILIZATION OF CHLORINE DIOXIDE IN AN AQUEOUS SOLUTION - XINIX TECHNOLOGY
Karsten Pedersen, University of Goteborg, Brent C. Reider, the XINIX Group, Inc., Columbus, OH
MAARS ISN'T A PLANET: IT'S A GLOBAL ASSURANCE SYSTEM
Penny H. Holeman, RBP, CBSP; Joseph Van Houten, RBP, CBSP, Johnson & Johnson, Raritan, NJ
DEVELOPMENT AND IMPLEMENTATION OF A COMPUTER BASED LABORATORY SAFETY TRAINING PROGRAM
Michelle L. Markley, National Institutes of Health, Bethesda, MD
ESTABLISHING & MAINTAINING SAFETY & HEALTH COMMITTEES IN A COMPLEX BIOMEDICAL RESEARCH ORGANIZATION
Rosamond Rutledge-Burns, CBSP, National Institutes of Health, Bethesda, MD
7:00 - 10:00 BANQUET - ST. LOUIS ARCH
WEDNESDAY, OCTOBER 20, 1999
7:00 - 10:00 REGISTRATION
7:00 - 8:00 CONTINENTAL BREAKFAST
8:30 - 8:35 INCOMING PRESIDENT'S MESSAGE
8:35 - 10:00 CONCURRENT SESSION A
DISINFECTION ROUDTABLE
Moderator: Benjamin Fontes, CBSP, Yale University, New Haven, CT; Mauren Best, Richard Fink, CBSP, Glenn Funk, Syed Sattar, Donald Vesley, RBP
8:35 - 10:00 CONCURRENT SESSION B
UN BIOSAFETY PROTOCOL ROUNDTABLE
Moderator: Penny Holeman, RBP, CBSP, Johnson & Johnson, Raritan, NJ; John Keddie, Suzanne Foti, Val Giddings
10:00 - 10:20 BREAK
______________________________________________________
10:20 - 12:00 PLENARY SESSION 8
Moderator: Deanna Robins
10:20 - 10:40
DEVELOPING SAFE GUIDELINES FOR WORKING WITH MACAQUES
Jonathon Richmond, RBP, Office of Health and Safety, CDC, Atlanta, GA
10:40 - 11:00
DESIGN AND UPGRADE OF A CONTAINMENT INSECTARY
Wen Lin and Ben Fontes, RBP, Yale University, New Haven, CT
11:00 - 11:20
CALIFORNIA STANDARD: BLOODBORNE PATHOGENS/SHARPS INJURY PREVENTION
Marilyn Misenhimer, RBP, CBSP, President, ABSA, University of Southern California, Los Angeles, CA
11:20 - 11:40
REPORTS OF THREE RECENT LABORATORY ACQUIRED INFECTIONS
Henry Mathews, Joanne J. Jones, Richard C. Knudsen, and Jonathan Y. Richmond, RBP, Office of Health & Safety, CDC, Atlanta, GA
11:40 - 12:00
BLOOD DONATIONS FOR RESEARCH PURPOSES: MANAGING THE PROCESS
Christina Z. Thompson, RBP, CBSP, Eli Lilly and Company, Indianapolis, IN
PRE-CONFERENCE COURSE DESCRIPTIONS
Limited Enrollment! Register Early to Assure a Seat
1. REVIEW COURSE FOR BIOSAFETY AND NATIONAL REGISTRY OF MICROBIOLOGISTS (NRM) EXAM IN BIOLOGICAL SAFETY MICROBIOLOGY
- 1:00 pm - 5:00 pm - Friday, 8:00 am - 5:00 pm - Saturday, Sunday
Instructors: Mary Cipriano, RBP, CBSP, Marian Downing, CBSP, Benjamin Fontes, CBSP, Glenn Funk, CBSP, and Christine Thompson, RBP, CBSP.
This new 2.5 day course will provide a comprehensive overview of all aspects of biological safety, with emphasis on the NRM Specialist Microbiologist Task List for Biological Safety Microbiology. The course will be of interest to both those individuals new to the field of biosafety, and to those individuals preparing to take the NRM Specialist Microbiologist (SM) exam in Biological Safety Microbiology. Principal instructors are Certified Biological Safety Professionals (CBSP) through ABSA but may have also obtained certification through the NRM as Specialist Microbiologists.
2. BIOSAFETY LEVEL 3 (BSL3) PROGRAMS: IN LABORATORIES, ANIMAL FACILITIES, AND THE FIELD - 1:00 pm - 5:00 pm - Friday
Karen Byers, RBP, CBSP, Dana-Farber Cancer Institute, Benjamin Fontes, CBSP, Yale University; Linda Wolfe, RBP, CBSP, elements in various settings (labs, animal facilities, field work), auditing practices and procedures, and administration of the program. Operating BSL3 programs for work with HIV, TB, and Arboviruses will be presented. Interactive exercises have been designed so that course participants will work in small groups to perform risk assessment, choose personal protective equipment, and evaluate exposure incidents. Sample manuals and a bibliography will be provided.
3. RISK ASSESSMENT
- 8:00 am - 5:00 pm - Saturday
Lynn Harding, CBSP, Biosafety Consultant, Diane O. Fleming, RBP, CBSP, Biosafety Consultant
This interactive workshop provides an opportunity to continue adding to the knowledge and skills necessary to perform the complex risk assessments required for the safety of employees using hazardous biological materials in this era of rapid technological development. The regulatory components of risk assessment will also be discussed. Using the case study approach, participants will interact to develop and understand the process of risk assessment as it relates to biosafety. This workshop will be of interest to biosafety officers, biosafety committee members, industrial hygienists or safety program managers who are responsible for biosafety programs and will focus on providing a practical framework for the performance of biohazard risk assessment. Please note that participants are expected to have a basic knowledge of biosafety procedures and containment practices.
4. BIOSAFETY LEVEL 3 (BSL3) DESIGN AND COMMISSIONING
- 8:00 am - 5:00 pm - Saturday
Esmeralda Prat-Party, RBP, Rockefeller University, Lee Alderman, Emory University, Janet S. Baum, Health Education& Research Associates
With the emergence or reemergence of some highly contagious aerosol-borne diseases the need for BSL3 facilities has increased. What do you need to consider when planning and designing a large BSL3 facility or a small two or three room facility placed in the midst of other laboratories? Once the facility is built, how do you determine that it is in fact a good containment area under normal operations and in emergency situations? If you are faced with these kinds of questions, then this course will help you answer them!
5. THE SCIENCE, TECHNIQUES, AND JARGON OF MOLECULAR BIOLOGY
- 8:00 am - 12:00 pm - Saturday
Patrick Condreay, Research Investigator, Department of Molecular Sciences, Glaxo Wellcome R&D
Our understanding of the details of biological processes at the molecular level has expanded at an astonishing rate making possible the easy genetic manipulation of various biological systems. Many of these experiments fall under the purview of the NIH Guidelines for Research with Recombinant DNA Molecules, and require review by Institutional Biosafety Committees. Thus it is important that members of these groups who are not practicing scientists be able to understand and communicate effectively with the scientist who submits a protocol. In this course we will examine the molecules, processes, and techniques involved in gene expression, and cover the basic techniques that are used in the construction of recombinant DNA molecules. The material is targeted for the biosafety professional who is not actively conducting laboratory research, yet requires a basic understanding of recombinant DNA methodology.
6. VENTILATION ISSUES FOR BIOSAFETY PROFESSIONALS
- 8:00 am - 12:00 pm - Saturday
Byron S. Tepper, CBSP, BioControl, Inc., Jerrold Koenigsberg, GPR/Sverdrup Corporation
This course will cover ventilation options encountered in the design and operation of chemical and biological general and containment laboratories. Topics will include constant volume vs. variable volume air systems; recommendations for laboratory air exchange rates; fume hood choice and fume hood exhaust rates; biological safety cabinet choice and re-circulation, capture and direct connection exhaust modes; ductwork and new ducting standards; exhaust location and treatment; and energy recovery. Time will be allotted at the end of the session for participant/faculty discussion.
7. INDUSTRIAL HYGIENE FOR BIOSAFETY PROFESSIONALS
- 8:00 am - 5:00 pm - Sunday
Matthew Finucane, University of Pennsylvania, Larry Gibbs, Stanford University
This course is designed to inform the biosafety professional of the basic health hazard recognition, evaluation and control issues to be considered when working with laboratories that use chemicals. Participants will gain an understanding of the fundamental principles of industrial hygiene - the anticipation, recognition, evaluation and control of chemical stressors - and the application of these factors in academic and industrial research laboratories. After a brief overview of the principles of industrial hygiene and toxicology, the instructors will present topics that must be considered in establishing the rudiments of a chemical hygiene program in the laboratory. Resources available to assist the biosafety professional in risk assessment and evaluation of general laboratory safety and health concerns will also be discussed.
8. BIOAEROSOLS
- 1:00 pm - 5:00 pm - Saturday
Jerry J. Tulis, Duke University Medical Center
This course will provide an understanding of the role of bioaerosols as related to human health. Current state-of-the-art information will be provided on the recognition of potential IAQ problems. Other areas to be covered include the methodology for the collection and analysis of biopollutants, preparation of investigation reports, remediation efforts and providing advice to clients. Participants will also gain an awareness of emerging consensus standards on the control of fungal amplification in building ventilation. An added feature of this course is its "workshop" format, which will feature presentations as well as equipment and laboratory demonstrations and exhibits.
9. ADVANCED DISINFECTION
- 8:00 am - 12:00 pm - Sunday
Richard Fink, CBSP, Massachusetts Institute of Technology, Syed Sattar, University of Ottawa, Donald Vesley, RBP, University of Minnesota
This course will assist the biosafety professional in building upon basic knowledge of the science of disinfection. Topics to be covered include: How disinfectants are tested and what that means to the end user; How to disinfect environmental surfaces (benches to floors) including contact time, how disinfectant properties effect the process, residual properties, and how to avoid spreading contamination; Disinfection vs. sterilization, and are there times when disinfection is not sufficient? There will also be coverage of special applications such as disinfection of fermentation processes, prions, and other hard to kill material. The course assumes that the student has a basic grounding in disinfection and disinfectants.
10. MEDICAL SURVEILLANCE
- 1:00 pm - 5:00 pm - Sunday
Gregg Kasting, CDC Occupational Health Clinic, DynCorp.
Immunizations and medical surveillance are important parts of health and safety programs, but are often surrounded by uncertainty both for medical and safety personnel. The first half of this course will provide an overview of these occupational health programs; the second half will consist of an in-depth question and answer session with the course leader. Specific examples participants will discuss include medical clearance into animal areas; B virus, HIV, and other agent post-exposure management; tuberculosis screening; hepatitis B and other immunization requirements; surveillance for specific disease agents; and baseline serum storage. The course will also include an overview of helpful reference material.
AMERICAN BIOLOGICAL SAFETY ASSOCIATION
42nd ANNUAL BIOLOGICAL SAFETY CONFERENCE - OCTOBER 17-20, 1999
REGISTRATION FORM
Please provide all information requested below as it should appear in our computer files. ( New member/1999
________________________________________________________________________________
Last Name First Name Middle Initial Guest/Spouse (if attending)
_________________________________________________________________________________
Affiliation Department Phone Fax
_________________________________________________________________________________
Street Address PO Box City State Zip
CONFERENCE FEES: Pre Oct 1 Post Oct 1 Amount Enclosed
ABSA Member $395 $445 $___________
Non-Member $495 $545 $___________
Single Day (Day ____________) $150 $150 $___________
Emeritus Member $240 $290 $___________
Student Registration $240 $290 $___________
Total $___________
Registration includes continental breakfasts, coffee breaks, 2 lunches, opening reception and closing dinner.
PRE-CONFERENCE COURSE Member Non-Member Amount Enclosed
1. Certification Review $500 $525 $___________
2. BSL3 Programs $135 $160 $___________
3. Risk Assessment $205 $225 $___________
4. BSL3 Design & Commissioning $205 $225 $___________
5. Molecular Biology $135 $160 $___________
6. Ventilation Issues $135 $160 $___________
7. Bioaerosols $135 $160 $___________
8. Industrial Hygiene $205 $225 $___________
9. Advanced Disinfection $135 $160 $___________
10. Medical Surveillance $135 $160 $___________
ABSA reserves the right to cancel course in which case the enrollment fee will be fully refunded to the applicant.
Additional Tickets
Additional Sit Down Lunch Ticket(s) $30 $ 30 $___________
Reception & Dinner $70 $ 70 $___________
Please note any special dietary needs:_____________________________________________________
___Check enclosed made out to the American Biological Safety Association. Checks must be drawn on a U.S. Bank in U.S. funds or they will be returned to sender.
Please charge my ? Visa ? MasterCard ? American Express
#__________________________ Exp Date:__________Signature:____________________________
INSTRUCTIONS: Guest/Spouse is invited to attend the Opening Reception. Guest/Spouse needs to purchase ticket for other events they attend. Payment or credit card must accompany form. Forms received without full payment will be returned to sender.
CANCELLATION POLICY: Cancellations received prior to October 1, 1999 - 90% refund. Cancellations received October 1 - 7, 1999 - 50% refund. Cancellations after October 7, 1999 - no refund. SUBSTITUTES ARE WELCOME with prior notification if possible.
MAIL TO: ABSA. 1202 Allanson Rd., Mundelein, IL 60060 or fax to 847-566-4580. Please note on charges we will need a signature on file.
HOTEL REGISTRATION
REGAL RIVERFRONT HOTEL
200 South 4th Street
St. Louis, MO 63102-1804
Phone 314.241.9500 / Fax 314.241.6171
Reservations: 800.222.8888
Rates: Single $129/Double $139
Cut-off date: September 23, 1999
42ndBiologicalSafetyConferenceProgram99Revised82399
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--=====================_935603698==_
Content-Type: text/plain; charset="us-ascii"
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
--=====================_935603698==_--
=========================================================================
Date: Wed, 25 Aug 1999 11:28:19 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: NFS 49 or not?
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Andrew -
At UCSF, we require the PIs to have their biosafety cabinets certified by an
outside vendor annually. I provide the names of three vendors without bias;
PIs can choose any of the three. One of these vendors is not
NSF49-certified; I leave him on the list because he has a long history of
good service here and I'm familiar with his work. My personal preference
would be for only NSF-certified certifiers.
-- Glenn
------------------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
University of California, San Francisco
Voice 415-476-2097
Fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Andrew Cockburn [mailto:acockbur@WVU.EDU]
Sent: Tuesday, August 24, 1999 4:24 PM
To: BIOSAFTY@mitvma.mit.edu
Subject: NFS 49 or not?
Hi all,
Before my arrival, different departments used different vendors to certify
their biosafety cabinets. Most vendors certified to NSF-49, but at least one
used "manufacturer's specifications", whatever that means.
This year I talked to an experienced vendor who certifies to NSF-49 and
negotiated a good price ($110 each) based on a volume discount. However one
department wants to use a different vendor (not a cost issue, they just want
to be different). This has the potential to raise overall costs to WVU
because of the loss of volume discounts.
How do other universities handle this: does the IBO choose a vendor and then
everyone has to use that vendor? What about not certifying to NSF-49 or
using personnel that are not NSF accredited?
How would you handle this: (1) let everyone use any vendor, (2) provide a
short list of acceptable vendors, or (3) negotiate a blanket order with one
vendor and tell everyone that they have to use it?
Andrew Cockburn, PhD
Director of Biological Safety
West Virginia University
Morgantown, WV 26506-9006
Telephone: 304-293-7157
FAX: 304-293-4529
Email: acockbur@wvu.edu
=========================================================================
Date: Wed, 25 Aug 1999 15:33:19 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Wolz, Sarah"
Subject: BL3 Lab with BL2 Lab as Access Zone
MIME-Version: 1.0
Content-Type: text/plain
Biosafetians:
We are in the process of reconfiguring some of our BL3 spaces into BL2 space
with direct entry through an inner single door into smaller BL3 labs. The
end result will be much like the concept presented in Dr. Richmond's
Anthology of Biosafety: I. Perspectives on Laboratory Design, Chapter 7
"Design of BL3 Laboratories".
Questions: in such a design (if you have the book, see page 114 or the
previous ABSA journal), where would you locate the changing
area--particularly the de-gowning area? Inside the BL3 part or just outside
in the BL2 area? The BL3 labs will be pretty small--2 of them, across a
dead-end BL2 corridor from each other. If you render me an opinion, would
you be so kind as to justify your opinion? I will need to do the same, and
am looking for some help in making my decisions...
Thanks from sunny Seattle! (well, at least this week)
Sarah Wolz
EH&S
PathoGenesis Corp.
201 Elliott Ave W.
Seattle, WA 98119
206-505-6953
swolz@
=========================================================================
Date: Thu, 26 Aug 1999 08:15:11 +0200
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Didier Breyer
Subject: New version of the Belgian Biosafety Server
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
WHAT'S NEW IN THE BELGIAN BIOSAFETY SERVER ?
New hardware, New softwares, and IMPROVED CONTENT.
Let's have a brief look ...
Chapter "Biosafety in Belgium" has been completely revised to give you
accurate and detailed information about the regulatory framework, the
advisory system and the administrative aspects related to the management of
biosafety in Belgium. The Belgian Biosafety Server not only provides full
text of all biosafety regulations, but aims also to be an online help to
scientists working in laboratories, greenhouses, animal husbandries and
large-scale units or involved in field tests and to investors and actors
involved in the commercialisation of GMO's on the EU market.
Chapter "Biosafety in European Union" gathers information about EU
legislation, European institutions and the regulatory framework in european
countries. No less than 39 Directives or Decisions can now be consulted
on-line, several of them being also available as PDF files.
Chapter "Biosafety in Other Countries" has also been revised and links the
visitor to those available Internet resources providing complementary
regulatory and scientific information in non-european countries.
The information contained on our Web site can also be reached through
specific regulatory topics:
- Revision of the EU Directive on the contained use of GMM's;
- Deliberate release and placing on the EU market of GMO's;
- Biosafety and Sustainable Development (specifically focusing on the
current negociation of the Biosafety Protocol);
- Safe transport of GMO's and pathogenic organisms;
- Antibiotic resistance genes in GMO's (listing all micro-organisms and
plants carrying an antibiotic resistance gene that have been notified
and/or approved for marketing in the European Union).
Antibiotic resistance is also the subject of a specific chapter aiming at
helping regulators or applicants to carry out risk assessment of GMO's
containing an antibiotic resistance gene, by providing information on the
genetic determinants encoding resistance and on their
clinical/environmental distribution when possible. These Web pages also
gather useful links to sites monitoring antimicrobial resistances and
describing antibiotic drugs.
And if you are looking for information for your risk assessment, simply go
to our "Laboratory Biosafety Resources" chapter. Hundreds of references and
links have been collected and sorted for you.
Last but not least, the Belgian Biosafety Server still provides you with a
list of forthcoming meetings/conferences/courses related to biosafety
(chapter "Forthcoming Events") and an additional keyword-based search
function helps to gather those page containing the keywords of interest.
We hope this short presentation of the new version of the Belgian Biosafety
Server did made your mouth water.
Welcome to and Enjoy your visit !
******************************************************
* Didier BREYER, Ph. D. *
* Biosafety expert *
* Belgian Biosafety Advisory Council *
* Service of Biosafety and Biotechnology *
* Institute of Public Health - Louis Pasteur *
* Rue Juliette Wytsmanstraat, 14 *
* B-1050 Brussels BELGIUM *
* Ph: 322-6425293 Fx: 322-6425292 *
* Email: dbreyer@sbb.ihe.be *
* Belgian Biosafety Server: *
******************************************************
=========================================================================
Date: Thu, 26 Aug 1999 09:01:25 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: BL3 Lab with BL2 Lab as Access Zone
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
We have recently become aware of a problem with our BL3 facility. There is
no changing area. Currently our people are changing in the BL2 area to
enter the BL3.
We have proposed a changing room(in the original layout be converted froma
store room back into a locker room. Access would then be from the outside
to an ante chamber to the locker room to the BL2 To the BL3.
Resistance to this proposal is high from th powers that be. They are not
buying it. The CDC has been of little use. I found a diagram that lays
out what we have proposed BUT, a changing area does not appear anywhere on
the diagram.
Thoughts on where I can get support for this position?
Bob
>Biosafetians:
>
>We are in the process of reconfiguring some of our BL3 spaces into BL2 space
>with direct entry through an inner single door into smaller BL3 labs. The
>end result will be much like the concept presented in Dr. Richmond's
>Anthology of Biosafety: I. Perspectives on Laboratory Design, Chapter 7
>"Design of BL3 Laboratories".
>
>Questions: in such a design (if you have the book, see page 114 or the
>previous ABSA journal), where would you locate the changing
>area--particularly the de-gowning area? Inside the BL3 part or just outside
>in the BL2 area? The BL3 labs will be pretty small--2 of them, across a
>dead-end BL2 corridor from each other. If you render me an opinion, would
>you be so kind as to justify your opinion? I will need to do the same, and
>am looking for some help in making my decisions...
>
>Thanks from sunny Seattle! (well, at least this week)
>
>Sarah Wolz
>EH&S
>PathoGenesis Corp.
>201 Elliott Ave W.
>Seattle, WA 98119
>206-505-6953
>swolz@
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Thu, 26 Aug 1999 11:00:03 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Francis Churchill
Subject: viral aerosols
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
This question was presented to me this morning by our Student Health Clinic
med staff. Is there reason to be concerned about aerosols of virus
(virii?) generated from spraying warts with liquid nitrogen? Evidently the
nitrogen is fairly energetic when sprayed.
I thank you in advance for your advice.
Francis
Alcohol and calculus don't mix. Never drink and derive.
Francis Churchill, IHIT
University of Vermont - Environmental Safety Facility
655D Spear Street, Burlington, VT 05405-3010
(802) 656-5405
fchurchi@zoo.uvm.edu
=========================================================================
Date: Thu, 26 Aug 1999 11:34:07 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ginger Brown
Subject: unsubscribe
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
At 08:07 AM 8/24/99 +0300, you wrote:
>-----Original Message-----
>From: Giovanni Ferraiolo ICGEB BIOSAFETY
>To: BIOSAFTY@MITVMA.MIT.EDU
>Date: =E9=E5=ED =F9=F0=E9 23 =E0=E5=E2=E5=F1=E8 1999 17:20
>Subject: *** ICGEB BIOSAFETY NEWS - AUGUST 1999 ***
>
>
>>____________________________________________________
>>
>> *** ICGEB BIOSAFETY NEWS - AUGUST 1999 ***
>>
>>____________________________________________________
>>
>>This message is to keep you updated with the news concerning
>>the ICGEB Biosafety WebPages and the activity of the Centre
>>in this issue. If you DO NOT want to receive further
>>messages, just REPLY typing "unsubscribe" in the "Subject"
>>field and your name will be automatically deleted from the
>>mailing list. ICGEB apologizes for any inconvenience due to
>>undesired posting.
>>____________________________________________________
>>
>>TWO BIOSAFETY WORKSHOPS IN THE ICGEB YEAR 2000 CALENDAR
>>
>>Two different workshops on biosafety and risk assessment for
>>the environmental release of genetically modified organisms
>>(GMOs) have been scheduled within the Year 2000 ICGEB
>>Calendar of Activities:
>>
>> *** 27-31 March 2000, Trieste, ITALY. Workshop:
>>"Biosafety 1. Science and Policy in Risk Assessment of
>>Transgenic Organisms: A Case Study Approach".
>>Organizers: George Tzotzos, UNIDO, Vienna, Austria; Gilbert
>>Howe, University of Bristol, UK; Giovanni Ferraiolo, ICGEB,
>>Trieste, Italy.
>>
>> *** 3-8 April 2000, Florence, ITALY. Workshop: "Biosafety
>>2. Advanced Research and Procedures: Case Studies for
>>Designated Experts".
>>Organizers: Marcello Broggio, Istituto Agronomico per
>>l'Oltremare, Florence, Italy; Giovanni Ferraiolo, ICGEB,
>>Trieste, Italy.
>>
>> Requests for information and applications directly to:
>>Ms. Micaela Di Blas, ICGEB, Padriciano 99, 34012 Trieste,
>>Italy. Tel: +39-040-3757333; Fax: +39-040-226555;
>>mailto:courses@icgeb.trieste.it
>>
>>
>>
>>* Biosafety 1: The workshop aims to offer a comprehensive
>>approach to biosafety and is directed to scientists
>>actively involved in biological risk assessment and/or
>>biotechnology regulations. ICGEB is dealing with biosafety
>>since its inception and since 1991 the Centre organizes
>>annual workshops on this issue attended, to date, from over
>>500 scientists involved in biosafety issues in more than 50
>>different countries.
>>
>>* Biosafety 2: Following the suggestions of many biosafety
>>focal-points and resource persons we have added, in our
>>agenda for the year 2000, an advanced workshop, directed
>>exclusively to officers in Governmental Agencies and/or
>>designated experts, working in the area of risk assessment
>>of GMOs at official level (governments, scientific
>>institutions, private sector etc.). This second workshop
>>aims to offer to scientists with advanced expertise in risk
>>assessment and/or biosafety regulative framework a dedicated
>>forum for advanced discussion and information sharing
>>
>> The general approach in both workshops will focus, with
>>special emphasis, on case study presentations and discussion
>>for the environmental release of genetically modified
>>organisms (GMOs). Detailed programs will be distributed when
>>available.
>>
>>Preference will be given to applicants from developing
>>countries. A limited number of grants will be available for
>>scientists from ICGEB Member Countries. These grants will
>>cover accommodation meals and incidentals for the duration
>>of the course; travel is NOT funded. Registration for the
>>Workshop is limited to 30 participants.
>>
>>Note . ICGEB is looking for Sponsors / Co-sponsors /
>>Contributors in order to recognize contributions toward
>>travel expenses of the participants. Interested
>>organizations may wish to contact the ICGEB
>>Secretariat in due time to facilitate the widest
>>participation to both workshops.
>>____________________________________________________
>>
>>For more information on ICGEB biosafety activities, please
>>contact:
>>
>>Giovanni Ferraiolo - mailto:biosafe@icgeb.trieste.it
>>ICGEB Biosafety, Programme Officer,
>>
>>International Centre for Genetic Engineering and
>>Biotechnology. Area Science Park, Padriciano 99 I-34012
>>Trieste (Italy). Tel: +39 040 3757364 - Fax: +39 040 226555
>>
>>ICGEB Biosafety WebPages:
>>____________________________________________________
>>
>>The International Centre for Genetic Engineering and
>>Biotechnology (ICGEB) is an autonomous, international,
>>intergovernmental organization formed by 61 signatory
>>countries of which 43 are full Member States. The Centre is
>>dedicated to advanced research and training in molecular
>>biology and biotechnology. The mandate of the ICGEB is to
>>promote the safe use of biotechnology world-wide and with
>>special regard to the needs of the developing world. Further
>>information on the ICGEB could be found on its web pages at
>>
>>____________________________________________________
>>
>>mlt2
>>unsubscribe
>
>
=========================================================================
Date: Thu, 26 Aug 1999 12:59:26 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: List Owner -- ICGEB BIOSAFETY NEWS - AUGUST 1999
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
The ICGEB BIOSAFETY NEWS has included the Biosafty list in its' mailing
list. A number of you have tried to unsubscribe -- you can't as you aren't
being directly mailed to, it is the list that is being mailed to.
So, the question is: do you want to have Biosafty receive this or not?
Please respond to me (rfink@mit.edu) directly rather then to the list in
general. Majority will rule.
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
=========================================================================
Date: Thu, 26 Aug 1999 13:02:43 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Biosafty Annual Dinner
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
It is getting close to that time - the ABSA conference and hence our annual
dinner. As usual, the dinner will be on Monday (the only open night).
What I need are some ideas regarding restaurants in St. Louis - any St.
Louisians on the list? Please contact me directly - rfink@mit.edu
Thanks. More to come once I get a list of possible dining places.
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
=========================================================================
Date: Thu, 26 Aug 1999 13:20:35 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Scott Alderman
Subject: CaBSA's Fall Symposium
Mime-Version: 1.0
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Carolinas Biological Safety Association, (Affiliate of ABSA), has planned its
Fall '99 Symosium for September 15 at GlaxoWellcome in RTP, NC. The title of
the program is "Community Response to Bioterrorism". The symposium will bring
together speakers who will represent the national, state, and local perspectives
on response to bioterrorism. Details are provided in the following attachement.
Feel free to contact me with any questions.
Scott Alderman
CaBSA President-Elect
919-684-8822
(See attached file: Fall99announcement.doc)
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=========================================================================
Date: Thu, 26 Aug 1999 17:11:13 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: David Mount
Subject: Biosafety officer recruiting
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Colleagues:
As chair of the IBC at the Univ of Arizona, I need to recruit a new
biosafety officer following the resignation of Sarah Palmer. I would be
very grateful to receive any information or thoughts you might have as to
suitable qualifications, salary range, required degree and discipline etc.
I am thinking seriously of someone with a biosafety background, a good
knowledge of microbiology and recombinant DNA research, and also, since we
are planning to build an automated, web-based system and supporting Oracle
database for PIs to use for MUA submission and lab licensing, some
experience or at least an interest in computional work. I am therefore
thinking that a Ph D with experience in these areas might be suitable. I
would appreciate any thoughts you might have to share. I will probably be
advertising by this medium once I have authorization for the position so
please do not make those kinds of inquiries yet. Thanks David Mount
PS has anyone else made such a web-based biosafety database?
David W. Mount, Professor
Dept of Molec and Cell Biol
Univ of AZ
Tucson, AZ 85721
office 520 621 3151 lab 520 621 4814
=========================================================================
Date: Fri, 27 Aug 1999 05:47:55 EDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: LSW Newsletter
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Hi NACHOs, NAOSMMs, DCHASs,, NSELAs, and SAFETYs,
The Lab Safety Workshop is looking for a volunteer to serve as managing
editor for our newsletter, Speaking of Safety. We currently publish three,
16-page issues per year. The managing editors responsibilities could include
gathering materials for the issues, writing copy, and desktop publishing to
produce a camera ready original for printing.
For more information, please contact me here or by phone. ... Jim
*****************************************************
James A. Kaufman, Director
The Laboratory Safety Workshop
Safety in Science Education
192 Worcester Road, Natick, MA 01760
508-647-1900 Fax: 508-647-0062 labsafe@
LSW is a national non-profit educational organization dedicated to making
health and safety an integral and important part of science education. Free
copies of our Laboratory Safety Guidelines, seminar schedule, Publications
List, AV-Lending Library List, and membership information are available on
request. The LABSAFETY-L discussion list is a public service of LSW.
******************************************************
=========================================================================
Date: Fri, 27 Aug 1999 09:43:19 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: Biosafety officer recruiting
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
Dear David: Consider serving as biosafety officer yourself as Chair of
the IBC. I have done it for many years here at our medical school and we
save valuable $ resources and still get the job done quite well through
an active IBC and Environmental SAfety and Resources [ESR] group. We
trained our ESR techs to monitor all facilities they service in doing
hood certification, chemical safety, etc for biohazard compliance. Our
IBC is very active and conducts unannounced biosafety reviews of all labs
with registered biological hazard work. If a faculty member or PI fails
to register required biohazards and get IBC approval for the work, he or
she is in deep DD. I give annual biosafety training OSHA mandates in BBPs
and general biosafety training once a year and we use my CD based PC
Biosafety training format for training all new hires as soon as they come
on board. We have a great support worker in our assoc, dean's office who
does all the paperwork and handles all the details for registering
training sessions, and biosafety compliance under my direction. Several
of our techs are certified biosafety cabinet certifiers and repair
trained and do all of our 111 biosafety cabinets by being paid out of our
inderect cost fund from grants. It saves
us a great deal of money and we do a pretty good job considering we have
several campuses, four hospitals and a very large registration of
biohazardous materials and projects as well as an entire building
dedicated to BSL-3 work ranging from drug resistant TB to prions, to
aerosol transmitted viruses and bacteria. We also have a huge volume of
blood related work ongoing. For this I get nothing in $ but am happy to
do it if we can avoid hiring a full time person to do a part-time job.
Joe Coggin, Jr. Ph.D. RBP, CBSP
Chair of M&I
On Thu, 26 Aug 1999, David Mount wrote:
> Colleagues:
>
> As chair of the IBC at the Univ of Arizona, I need to recruit a new
> biosafety officer following the resignation of Sarah Palmer. I would be
> very grateful to receive any information or thoughts you might have as to
> suitable qualifications, salary range, required degree and discipline etc.
> I am thinking seriously of someone with a biosafety background, a good
> knowledge of microbiology and recombinant DNA research, and also, since we
> are planning to build an automated, web-based system and supporting Oracle
> database for PIs to use for MUA submission and lab licensing, some
> experience or at least an interest in computional work. I am therefore
> thinking that a Ph D with experience in these areas might be suitable. I
> would appreciate any thoughts you might have to share. I will probably be
> advertising by this medium once I have authorization for the position so
> please do not make those kinds of inquiries yet. Thanks David Mount
>
> PS has anyone else made such a web-based biosafety database?
>
> David W. Mount, Professor
> Dept of Molec and Cell Biol
> Univ of AZ
> Tucson, AZ 85721
> office 520 621 3151 lab 520 621 4814
>
=========================================================================
Date: Fri, 27 Aug 1999 10:03:47 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: David Mount
Subject: Re: Biosafety officer recruiting
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Joe - Thank you for the advice. I am very impressed and will give your
proposal serious thought. David
>Dear David: Consider serving as biosafety officer yourself as Chair of
>the IBC. I have done it for many years here at our medical school and we
>save valuable $ resources and still get the job done quite well through
>an active IBC and Environmental SAfety and Resources [ESR] group. We
>trained our ESR techs to monitor all facilities they service in doing
>hood certification, chemical safety, etc for biohazard compliance. Our
>IBC is very active and conducts unannounced biosafety reviews of all labs
>with registered biological hazard work. If a faculty member or PI fails
>to register required biohazards and get IBC approval for the work, he or
>she is in deep DD. I give annual biosafety training OSHA mandates in BBPs
>and general biosafety training once a year and we use my CD based PC
>Biosafety training format for training all new hires as soon as they come
>on board. We have a great support worker in our assoc, dean's office who
>does all the paperwork and handles all the details for registering
>training sessions, and biosafety compliance under my direction. Several
>of our techs are certified biosafety cabinet certifiers and repair
>trained and do all of our 111 biosafety cabinets by being paid out of our
>inderect cost fund from grants. It saves
>us a great deal of money and we do a pretty good job considering we have
>several campuses, four hospitals and a very large registration of
>biohazardous materials and projects as well as an entire building
>dedicated to BSL-3 work ranging from drug resistant TB to prions, to
>aerosol transmitted viruses and bacteria. We also have a huge volume of
>blood related work ongoing. For this I get nothing in $ but am happy to
>do it if we can avoid hiring a full time person to do a part-time job.
>
>Joe Coggin, Jr. Ph.D. RBP, CBSP
>Chair of M&I
>
>
>
>
>
>
>
>
>
>On Thu, 26 Aug 1999, David Mount wrote:
>
>> Colleagues:
>>
>> As chair of the IBC at the Univ of Arizona, I need to recruit a new
>> biosafety officer following the resignation of Sarah Palmer. I would be
>> very grateful to receive any information or thoughts you might have as to
>> suitable qualifications, salary range, required degree and discipline etc.
>> I am thinking seriously of someone with a biosafety background, a good
>> knowledge of microbiology and recombinant DNA research, and also, since we
>> are planning to build an automated, web-based system and supporting Oracle
>> database for PIs to use for MUA submission and lab licensing, some
>> experience or at least an interest in computional work. I am therefore
>> thinking that a Ph D with experience in these areas might be suitable. I
>> would appreciate any thoughts you might have to share. I will probably be
>> advertising by this medium once I have authorization for the position so
>> please do not make those kinds of inquiries yet. Thanks David Mount
>>
>> PS has anyone else made such a web-based biosafety database?
>>
>> David W. Mount, Professor
>> Dept of Molec and Cell Biol
>> Univ of AZ
>> Tucson, AZ 85721
>> office 520 621 3151 lab 520 621 4814
>>
David W. Mount, Professor
Dept of Molec and Cell Biol
Univ of AZ
Tucson, AZ 85721
office 520 621 3151 lab 520 621 4814
=========================================================================
Date: Fri, 27 Aug 1999 20:36:57 EDT
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: Re: BIOSAFTY Digest - 25 Aug 1999 to 26 Aug 1999
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Please send information directly to me about how to change the digest format
from "mim" to "txt". Thanks. I can't read it when it is in the digest.
... Jim
=========================================================================
Date: Mon, 30 Aug 1999 10:14:16 -0400
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Bioaerosols
MIME-Version: 1.0
Content-Type: multipart/mixed; boundary="----_=_NextPart_000_01BEF2F1.F25FE5E6"
This message is in MIME format. Since your mail reader does not understand
this format, some or all of this message may not be legible.
------_=_NextPart_000_01BEF2F1.F25FE5E6
Content-Type: text/plain
I thought I would share this link with the group.
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
------_=_NextPart_000_01BEF2F1.F25FE5E6
Content-Type: application/octet-stream;
name="wjkaerob.htm"
Content-Transfer-Encoding: quoted-printable
Content-Disposition: attachment;
filename="wjkaerob.htm"
Content-Location: ATT-0-7CF4F6A3E25ED3118FF40008C7B130DF-w
jkaerob.htm
=0A=
AEROBIOLOGICAL ENGINEERING -- Airborne Pathogen Control =
Systems
INTRODUCTION
Aerobiological Engineering is the art and =
science of designing buildings and systems for the control of airborne =
pathogens and reduction of respiratory infections in indoor =
environments, including commercial buildings, hospitals, and =
residences. Aerobiology is the study of microorganisms in the air which =
may be detrimental to human health. Included among these organisms are =
viruses, bacteria and fungi.
The air can be full of transient populations of =
microorganisms, but none actually live in the air. Most microbes die =
off in the outdoor air as a result of sunlight, temperature extremes, =
dehydration, oxygen and pollution. Spores and some environmental =
bacteria are naturally more resistant and can occur outdoors seasonally =
in high concentrations.The controlled climate of the indoors favors the =
survival and transmission of contagious human pathogens as well as some =
outdoor fungi and bacteria. Since people spend 93% of their time =
indoors, the solution to the problem of most airborne infections, =
therefore, lies in engineering control of the aerobiology of the indoor =
environment.
AEROBIOLOGICAL ENGINEERING RESEARCH =
TOPICS
AIRBORNE=
PATHOGEN DATABASE
BIOAEROSOLS AND =
BIOAEROSOL DYNAMICS
FUNGI AND =
BACTERIA IN VENTILATION SYSTEMS
LEGIONNAIRES =
DISEASE
THE EVOLUTION OF =
AIRBORNE PATHOGENS
THE SPREAD OF =
DISEASE IN OFFICE BUILDINGS
EPIDEMIOLOGY =
FOR ENGINEERS
DNA AND THE =
MICROWAVE EFFECT
SICK BUILDING =
SYNDROME
AIRLINERS -- HOW =
SAFE IS THE AIR?
BIOAEROSOL =
SAMPLING AND DETECTION
LIST OF AIRBORNE PATHOGENS
CURRENT RESEARCH PROJECTS & ABSTRACTS
EQUIPMENT & SERVICES =
LINKS
AIRBORNE PATHOGEN CONTROL =
TECHNOLOGIES
CURRENT
DEVELOPMENTAL
ISOLATION =
SYSTEMS
PHOTOCATALYTIC =
OXIDATION
AIR =
FILTRATION
AIR =
OZONATION
ULTRAVIOLET =
IRRADIATION
CARBON =
ADSORPTION
OUTDOOR AIR =
PURGING
PASSIVE SOLAR =
EXPOSURE
ELECTROSTATIC =
PRECIPITATION
ULTRASONIC =
ATOMIZATION
NEGATIVE AIR =
IONIZATION
MICROWAVE =
ATOMIZATION
VEGETATION
PULSED =
LIGHT
ARTICLES
Air=
borne Respiratory Diseases and Mechanical Systems for Control of =
Microbes
by W. J. Kowalski and William Bahnfleth
HPAC, July 1998, pp34-48.
Bact=
ericidal Effects of High Airborne Ozone Concentrations on =
Escherichia coli and Staphylococcus aureus
by W. J. Kowalski, W. P. Bahnfleth, and T. S. Whittam
Ozone Science & Engineering v20, pp 205-221.
Filtra=
tion of Microorganisms: Modeling and Prediction
by W. J. Kowalski, William Bahnfleth, and T. S. Whittam
ASHRAE Convention, Seattle 1999
Media disposal
We've got a small problem with media disposal and I was wondering
if anyone else had addressed this issue. I've a researcher who is
having his employees pour used, autoclaved media straight into a
dumpster. The media consists of:
LB plates: 1% Tryptone, 0.5% Yeast extract, 1% NaCl, 1.5%
Agar
and
LB medium: 1% Tryptone, 0.5% Yeast extract, 1% NaCl
Before use they add chloramphenicol (stock solution: 20 mg/ml in
ethanol) at below 70 degrees C after autoclaving. (The
chloramphenicol should be deactivated when they autoclave it after
use.)
It's harmless, but it stinks (like hot media does) and we get
many, many complaints - some from others in this building (not a bio
building) and the folks who empty the dumpster (our own facilities
employees). He's been doing this elsewhere on campus without problems
(so he says), but has opened a new operation in a different
building.
So far I've suggested that he:
pour the media into 5 gallon buckets and seal the lid
prior to disposal in the dumpster (he is unwilling to do
this);
Pour the hot media down the drain with copious amounts of
hot water (turns out they toss all sorts of things in when
autoclaving - gloves, etc. - so it's not just media. Even copious
amounts of hot water won't wash a glove down the drain);
and that they autoclave in autoclave bags and place them
in the dumpster (gently). (No answer so far on this one).
I realize that we're asking him to alter his procedures for
disposing of a non-hazardous material merely to accommodate a few
people who find the odor offensive, but I find his reluctance to be a
good neighbor frustrating.
Any suggestions?
Robin
W. Robert Newberry, IV CIH, CHMM
Director, Environmental Health and Safety
Clemson University
wnewber@clemson.edu ehs@clemson.edu
--============_-1270547233==_ma============--
=========================================================================
Date: Tue, 2 Nov 1999 16:57:05 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ninni Jacob
Subject: Treatment and Disposal of Regulated Medical Waste
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/enriched; charset="us-ascii"
According to our state regulations we can treat, destroy and dispose
regulated medical waste on-site or off-site.
Do any of you treat and destroy Regulated Medical Waste on-site?
I would like information on waste other than
sharps, liquids(including human blood), human and animal pathological
wastes.
Specifically, I would like information on Cultures and Stocks oof
Infectious materials including HIV.
The acceptable technologies (according to our State Regs) include:
1. Incineration
2. Chemical disinfection
OR
3. Steam sterilisation followed by grinding and
shredding.
Do any of you have grinders or shredders?
Is it cost effective to do that on-site as opposed to autoclaving and
sending off-site for destruction?
As always, thanks a lot for your input.
Ninni Jacob, CHP
Radiation and Biological Safety Officer
Office of Risk Management
Brown University - Box 1914
164 Angell Street
Providence, RI 02912
Tel:401 863 1738
Fax:401 863 7676
email: Ninni_Jacob@brown.edu
=========================================================================
Date: Tue, 2 Nov 1999 14:10:08 -1000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Thomas Goob
Subject: Re: Treatment and Disposal of Regulated Medical Waste
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/enriched; charset="us-ascii"
We have a "hammermill" which grinds/shreds and chemically treats our
regulated waste with a 12.5% Sodium Hypochlorite solution. From a
cost-effective standpoint we are looking at big $$$ savings. However,
this is not without a lot of work and efforts to include:
1. An involved preventive maintenance program for the equipment.
2. Development of standard operating procedures.
3. Training of employees on the equipment.
4. Lockout/tagout procedures.
5. Ventilation requirements.
6. Discharge permits and monitoring program.
7. Segregation of waste which can not be processed.
8. Etc.
We are still working out the bugs on a number of the above listed issues.
I am hopeful that once worked out that this process will not cause me as
many headaches as it has thus far.
Thomas C. Goob, MPH, MBA, CSP
Diagnostic Laboratory Services, Inc.
tgoob@dls.
PS: The system we are using is called the MST 300L System, manufactured
by MedSafe Technologies in Indiana. If anyone else is using this please
contact me. I would like to share experiences.
At 04:57 PM 11/02/1999 -0500, Ninni Jacob wrote:
>>>>
According to our state regulations we can treat, destroy and
dispose regulated medical waste on-site or off-site.
Do any of you treat and destroy Regulated Medical Waste on-site?
I would like information on waste other than
sharps, liquids(including human blood), human and animal pathological
wastes.
Specifically, I would like information on Cultures and Stocks oof
Infectious materials including HIV.
The acceptable technologies (according to our State Regs) include:
1. Incineration
2. Chemical disinfection
OR
3. Steam sterilisation followed by grinding and
shredding.
Do any of you have grinders or shredders?
Is it cost effective to do that on-site as opposed to autoclaving and
sending off-site for destruction?
As always, thanks a lot for your input.
Ninni Jacob, CHP
Radiation and Biological Safety Officer
Office of Risk Management
Brown University - Box 1914
164 Angell Street
Providence, RI 02912
Tel:401 863 1738
Fax:401 863 7676
email: Ninni_Jacob@brown.edu
complaints - some from others in this building (not a bio building) and
>the folks who empty the dumpster (our own facilities employees). He's been
>doing this elsewhere on campus without problems (so he says), but has
>opened a new operation in a different building.
> So far I've suggested that he:
>
>
>pour the media into 5 gallon buckets and seal the lid prior to disposal in
>the dumpster (he is unwilling to do this);
>
>
>
>
>Pour the hot media down the drain with copious amounts of hot water (turns
>out they toss all sorts of things in when autoclaving - gloves, etc. - so
>it's not just media. Even copious amounts of hot water won't wash a glove
>down the drain);
>
>
>
>
>and that they autoclave in autoclave bags and place them in the dumpster
>(gently). (No answer so far on this one).
>
>
> I realize that we're asking him to alter his procedures for disposing of
>a non-hazardous material merely to accommodate a few people who find the
>odor offensive, but I find his reluctance to be a good neighbor
>frustrating.
> Any suggestions?
>
>Robin
>
> W. Robert Newberry, IV CIH, CHMM
> Director, Environmental Health and Safety
> Clemson University
>
> wnewber@clemson.edu ehs@clemson.edu
>
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Wed, 3 Nov 1999 10:18:03 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Clark
Subject: Re: Media disposal
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/html; charset="us-ascii"
How about transferring him to Texas Tech, along with the old TIWET crew
?
At 03:41 PM 11/2/99 -0500, you wrote:
We've got a small problem with media disposal
and I was wondering if anyone else had addressed this issue. I've a
researcher who is having his employees pour used, autoclaved media
straight into a dumpster. The media consists of:
LB plates: 1% Tryptone, 0.5% Yeast extract, 1% NaCl, 1.5% Agar
and
LB medium: 1% Tryptone, 0.5% Yeast extract, 1% NaCl
Before use they add chloramphenicol (stock solution: 20 mg/ml in ethanol)
at below 70 degrees C after autoclaving. (The chloramphenicol
should be deactivated when they autoclave it after use.)
It's harmless, but it stinks (like hot media does) and we get many, many
complaints - some from others in this building (not a bio building) and
the folks who empty the dumpster (our own facilities employees). He's
been doing this elsewhere on campus without problems (so he says), but
has opened a new operation in a different building.
So far I've suggested that he:
pour the media into 5 gallon buckets and seal the lid prior to
disposal in the dumpster (he is unwilling to do this);
Pour the hot media down the drain with copious amounts of hot water
(turns out they toss all sorts of things in when autoclaving - gloves,
etc. - so it's not just media. Even copious amounts of hot water won't
wash a glove down the drain);
and that they autoclave in autoclave bags and place them in the
dumpster (gently). (No answer so far on this one).
I realize that we're asking him to alter his procedures for disposing of
a non-hazardous material merely to accommodate a few people who find the
odor offensive, but I find his reluctance to be a good neighbor
frustrating.
Any suggestions?
Robin
W. Robert Newberry, IV CIH, CHMM
Director, Environmental Health and Safety
Clemson University
wnewber@clemson.edu ehs@clemson.edu
=========================================================================
Date: Wed, 3 Nov 1999 10:59:18 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Don Callihan
Subject: Re: Media disposal
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
From: Don Callihan@BDX on 11/03/99 10:59 AM
The media you describe contain peptones that should pose no hazard to anyone.
The animal origins of the material is known by the manufacturer, so contact them
if there is concern for an MSDS.
Unless the media contains blood, UNINOCULATED media can and should be disposed
as regular trash.
Media that has been inoculated may be autoclaved and disposed as regular trash,
unless state or local regulations require otherwise.
I agree that media containing blood, human or animal origin, should be handled
as biohazardous waste, collected in red bags and autoclaved before disposal.
For any media, once autoclaved properly, all manner of living things should be
dead and the smell, although unpleasant, should not be strong or noxious, and
certainly wouldn't smell any worse than the average dumpster. You should ask if
the autoclave that is being used has been validated for appropriate killing and
that the cycle time is long enough, the autoclave is not overloaded, and that
bags are not sealed within the autoclave. Time, temperature, proper loading, and
allowing access of the steam to all areas of the load will all contribute to
rendering everything dead. As biosafety officer, you should consider having a
detailed SOP for training and routine operation of all autoclaves in your
facility. Also included should be validation requirements, performance measures,
and routine maintenance procedures.
If the smell of disposed media is so overpowering that you get complaints, it is
possible that some microbes are surviving the "autoclave" process and continue
to grow and product metabolic biproducts while sitting in the dumpster or other
holding area. It may be that the materials are not being autoclaved at all.
Hope these comments help.
Don Callihan, Ph.D.
BD Biosciences
Sparks, MD
410.773.6684
=========================================================================
Date: Wed, 3 Nov 1999 11:36:41 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Robin Newberry
Subject: Re: Media disposal
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
>
>If the smell of disposed media is so overpowering that you get
>complaints, it is
>possible that some microbes are surviving the "autoclave" process and continue
>to grow and product metabolic biproducts while sitting in the
>dumpster or other
>holding area. It may be that the materials are not being autoclaved at all.
The media is definitely being autoclaved, and has the rather
unpleasant odor almost all media have when hot. In the past, all that
went into this dumpster was almost exclusively office waste, so the
smell is a new feature.
As is often the case, the actual hazard of the material takes second
place to the politics of the workplace. This operation is new to this
building, and the building (although historically a lab building
(mostly chemistry)) has a high concentration of non-technical office
employees who simply aren't used to the odor of a bio lab.
Robin
W. Robert Newberry, IV CIH, CHMM
Director, Environmental Health and Safety
Clemson University
wnewber@clemson.edu ehs@clemson.edu
=========================================================================
Date: Wed, 3 Nov 1999 09:29:23 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Petty, Carol"
Subject: New
MIME-Version: 1.0
Content-Type: text/plain
I just recently joined this email group and would like to introduce myself.
I am Carol Petty and my background is industrial hygiene and safety. I am
now becoming a biosafety officer for a research facility in New Mexico. I
would welcome any suggestions or comments from those who have been in this
field for awhile in the best way to set up the program and also the medical
surveillance. For example, are there any good references on the vaccines
available for agents. I am using the CDC Biosafety in Microbiological and
Biomedical Laboratories primarily at the moment. Thanks.
Carol L. Petty, C.I.H., C.S.P.
Industrial Hygienist
Phone: (505) 845-1076
Fax: (505) 845-1174
email: cpetty@
=========================================================================
Date: Wed, 3 Nov 1999 11:47:03 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stefan Wagener
Subject: CDC proposed shipping rules!
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FYI
I have attached the text of the proposed CDC rule on packaging and handling
of infectious substances and select agents.
Stefan :-)
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WAIS Document Retrieval[Federal Register: October 28, 1999 (Volume 64, =
Number 208)]
[Proposed Rules] =20
[Page 58022-58031]
From the Federal Register Online via GPO Access [wais.access.]
[DOCID:fr28oc99-30] =20
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D
-----------------------------------------------------------------------
DEPARTMENT OF HEALTH AND HUMAN SERVICES
Centers for Disease Control and Prevention
42 CFR Part 72
RIN 0920-AA02
=20
Packaging and Handling of Infectious Substances and Select Agents
AGENCY: Centers for Disease Control and Prevention (CDC), HHS.
ACTION: Notice of proposed rulemaking.
-----------------------------------------------------------------------
SUMMARY: The Centers for Disease Control and Prevention proposes to
[[Page 58023]]
amend the regulations concerning the interstate shipment of infectious=20
substances in order to clarify and expand the existing requirements for=20
proper packaging and handling of these agents. One purpose of the=20
proposed rule is to ensure that all biological materials that are known=20
or suspected of containing an infectious substance are packaged for=20
interstate shipment to minimize the potential for leakage of contents=20
that could contaminate the environment or come into direct physical=20
contact with persons handling such packages during transit. A second=20
purpose is to insure receipt of certain infectious substances. This new=20
regulation will harmonize CDC regulations with other Federal agencies'=20
regulations and with international regulations.
It also updates the requirements for facilities transferring or=20
receiving select agents, incorporating by reference the 4th edition of=20
the CDC/NIH publication Biosafety in Microbiological and Biomedical=20
Laboratories.
DATE: Written comments must be received on or before December 27, 1999.=20
Written comments on the proposed information collection requirements=20
should also be submitted on or before December 27, 1999.
ADDRESSES: Mail written comments to the following address: Nashandra=20
Hayes, Office of Health and Safety, Centers for Disease Control and=20
Prevention, 1600 Clifton Road, Mail Stop--FO5, Atlanta, Georgia 30333.
Mail written comments on the proposed information collection=20
requirements to: Wendy Taylor, Office of Information and Regulatory=20
Affairs, Office of Management and Budget, New Executive Office=20
Building, 725 17th Street, NW, rm. 10235, Washington, DC 20503, Att.:=20
Desk Officer for CDC.
FOR FURTHER INFORMATION CONTACT: Dr. Jonathan Y. Richmond or Dr.=20
Richard Knudsen, Office of Health and Safety, Centers for Disease=20
Control and Prevention, 1600 Clifton Road, NE, Mail Stop F05, Atlanta,=20
Georgia 30333; telephone (404) 639-2453 or 639-3235, respectively.
SUPPLEMENTARY INFORMATION:
Revised Proposed Notice of Rulemaking
Replaces NPRM at 55 FR 7678, March 2, 1990.
I. Background
Under 42 U.S.C. 264, the Department of Health and Human Services is=20
authorized to promulgate regulations to prevent the introduction,=20
transmission and spread of communicable diseases from foreign countries=20
and between the states. Authority was given to CDC in 1971 to regulate=20
the interstate shipment of infectious substances. The current=20
regulations are at 42 CFR part 72. The regulations provide requirements=20
for minimum packaging and labeling for biological products and=20
diagnostic specimens, and include a list of infectious agents for which=20
special tracking is required. These regulations were last updated in=20
1980.
A Notice of Proposed Rulemaking (NPRM) was published in the Federal=20
Register on March 2, 1990 (55 FR 7678), to update the existing=20
packaging requirements for infectious substances. Impetus for that NPRM=20
came from postal workers and members of Congress who expressed concerns=20
about the potential risk of exposure to infectious agents for people=20
who handle improperly packaged or damaged packages of biomedical=20
material during transit. Persons shipping these materials also stated=20
that some definitions in the 1980 regulation were unclear. There had=20
also been changes in the list of infectious agents that required=20
notification of receipt.
Comments on the 1990 NPRM focused on two major issues. Numerous=20
parties, including United States Postal Service workers, submitted=20
comments regarding the transport of clinical specimens for diagnostic=20
studies. Several parties encouraged CDC to harmonize the proposed=20
regulation with the international shipping regulations.
Several government agencies and industry groups, in addition to=20
CDC, regulate the packaging, labeling and shipment of infectious=20
materials within the United States and internationally.
The Department of Transportation (DOT) Hazardous Materials=20
regulations, at 49 CFR parts 171-180, regulate the interstate=20
transportation by surface or air of infectious substances, medical=20
waste, chemical and radioactive materials. That regulation does not=20
apply to the transport of clinical or diagnostic specimens, unless=20
specifically known to contain an infectious substance (49 CFR 173.134).
The United States Postal Service (USPS) regulates the=20
shipment, by U.S. mail, of etiologic agents, infectious substances,=20
clinical specimens, biological products, and sharps (e.g., contaminated=20
needles and other sharp medical materials) and unsterilized containers=20
(39 CFR and Domestic Mail Manual C023, Etiologic Agent Preparations,=20
Clinical Specimens, and Biological Products; and International Mail=20
Manual 135 Mailable Dangerous Goods).
The Department of Labor, Occupational Safety and Health=20
Administration (OSHA), at 29 CFR 1910.1030, Occupational Exposure to=20
Bloodborne Pathogens, regulates the worker safety aspects of the=20
handling, packaging and transport of human blood and body fluids,=20
unfixed tissues, organs and cell cultures, and other fluids from humans=20
and animals infected or possibly infected with bloodborne pathogens.
The Department of Commerce (DOC) maintains a list of=20
controlled items, including certain microorganisms that cannot be=20
exported from the U.S. (15 CFR parts 768-799). The DOC recommends that=20
shippers follow the CDC regulation for packaging when a shipment is=20
allowed to a foreign country.
The United Nations Committee of Experts on the Transport=20
of Dangerous Goods makes recommendations on the international transport=20
of infectious substances and clinical specimens. These recommendations=20
are included in the International Civil Aeronautics Organization (ICAO)=20
technical instructions, which have been adopted by the International=20
Air Transport Association (IATA).
--ICAO publishes Technical Instructions for the Safe Transport of=20
Dangerous Goods by Air, based on the United Nations (UN)=20
recommendations for the domestic and international transport of=20
infectious substances and clinical (diagnostic) specimens.
--IATA publishes the Dangerous Goods Regulations (DGR), which further=20
describe for IATA member airlines, the national and international=20
recommendations for air transport of infectious substances and clinical=20
(diagnostic) specimens. The IATA DGR are followed by the domestic and=20
international member airlines.
CDC's regulation, currently at 42 CFR part 72, provides packaging=20
and labeling requirements for shipments of infectious materials. There=20
are several reasons why CDC regulates this area in addition to the=20
other agencies listed above. The focus of the CDC regulation is on=20
protection of the public health by minimizing the potential for (1)=20
Direct physical contact with package contents by persons handling such=20
packages during transit, (2) Contamination of the environment, and (3)=20
The spread of disease into the community. The CDC regulations serve by=20
filling the gaps where there is no governance, by complementing the=20
requirements of other agencies where there is overlapping authority,=20
and by providing CDC as a central reporting authority assures=20
availability of CDC's infectious
[[Page 58024]]
disease expertise to assist in the response when packages are damaged.
Although there had been some review of the requirements of other=20
agencies when developing the 1990 NPRM, there had not been any=20
comprehensive attempt to harmonize the various requirements. When=20
comments to the 1990 NPRM were reviewed, it became clear that there was=20
confusion among shippers and handlers as to how all the various=20
requirements of other agencies related to the CDC regulations. Because=20
of substantive differences in the requirements and use of different=20
terminology, there was a clear need to harmonize the various=20
requirements.
In response to the comments on the 1990 NPRM, and as part of the=20
regulatory reform/reinventing government initiative, CDC has=20
collaborated with the other agencies and groups to prepare revised=20
proposed CDC regulations that are in harmony with the other=20
requirements, thereby reducing the burden on shippers while still=20
maintaining, or even improving, packaging standards to protect the=20
public health. In some instances, one or more of the other agencies/
groups will also be revising their requirements as part of our joint=20
effort to achieve complementary regulations. We invite specific comment=20
on any requirements contained in the proposed CDC regulations which are=20
thought to be inconsistent or unclear in relation to the requirements=20
of any other regulatory authority.
CDC also serves as a Center for Applied Biosafety and Training for=20
the World Health Organization (WHO) and for the UN. In conjunction with=20
the National Institutes of Health, CDC has participated in developing=20
revised international guidelines for the shipment of infectious=20
materials and diagnostic specimens. This NPRM also reflects the=20
recommendations of the WHO biosafety advisory group, as published in=20
1997, in Guidelines for the Safe Transport of Infectious Substances and=20
Diagnostic Specimens.
As a result of this extensive collaboration, significant changes=20
have been made to the 1990 NPRM, and a new NPRM is being published to=20
provide the opportunity for laboratories and other shippers of=20
infectious materials, persons who transport or handle packages, public=20
health officials and other affected parties to comment on these=20
proposed regulations to ensure that the final regulations are both=20
complementary to other packaging and shipping requirements and=20
protective of the public health.
CDC believes these regulations will not be an additional burden to=20
shippers because shippers interested in ensuring the integrity of their=20
packages are already utilizing comparable packaging. These regulations=20
will help to ensure that all shippers are aware of and utilize=20
appropriate packaging when shipping infectious substances, thereby=20
protecting the public health.
Comparison of CDC's Proposed Packaging and Labeling Requirements=20
With Other Agencies' and Groups' Packaging and Labeling=20
Requirements
This NPRM proposes packaging and labeling requirements for: (1)=20
Clinical specimens because they may contain infectious agents, and (2)=20
materials known or suspected of containing infectious substances or=20
toxins.
Table 1 shows which types of infectious materials are covered by=20
each regulating authority and the scope of that coverage. As noted in=20
the table, no single agency covers all aspects regarding the shipment=20
of infectious substances.
Table 1.--Infectious Substances: Comparison of the CDC NPRM \1\ With =
OSHA,\2\ DOT,\3\ USPS,\4\ and IATA \5\
Packaging and Labeling =
Requirements
-------------------------------------------------------------------------=
---------------------------------------
=
Regulations
Requirements CDC NPRM \1\ =
-------------------------------------------------------
OSHA \2\ =
DOT \3\ USPS \4\ IATA \5\
-------------------------------------------------------------------------=
---------------------------------------
Infectious materials:
Biological products................... + + =
-\6\ + +
Clinical (diagnostic) specimens....... + + =
-\6\ + +
Cultures and reference stocks......... + + =
+ + +
Packaging materials:
Watertight primary receptacle......... + + =
+ + +
Absorbent material.................... + na =
+ + +
Watertight secondary packaging........ + na =
+ + +
List of contents...................... + na =
+ -\7\ +
Outer packaging....................... + na =
+ + +
Packaging performance standards....... + na =
+ + +
Packaging labels:
Infectious substance/biohazard symbol + + =
+ + +
label................................
Shipping label,\8\ outer packaging.... + na =
-\9\ + +
Shipping label, secondary packaging... + na =
na + na
Tracking special infectious substances.... + na =
na na na
Terminology............................... + + =
+ + +
Shipping modes covered.................... All All =
All Mail only Air only
-------------------------------------------------------------------------=
---------------------------------------
Legend: + =3D same or very similar to the CDC NPRM; - =3D significantly =
different from the CDC NPRM; na =3D not
addressed in regulation.
\1\ CDC: Centers for Disease Control and Prevention, 42 CFR Part 72 as =
proposed in this NPRM.
\2\ OSHA: Occupational Safety and Health Administration, 29 CFR =
1910.1030.
\3\ DOT: Department of Transportation, 49 CFR Parts 171-180.
\4\ USPS: United States Postal Service, Domestic Mail Manual CO23.
\5\ IATA: International Air Transport Association, Dangerous Goods =
Regulations.
\6\ Only those biological products and clinical specimens known to =
contain infectious substances are covered
under 49 CFR 173.134.
\7\ USPS requires a list of contents (manifest) for all sharps mailing =
containers, but only requires a list of
contents for other items sent via air transportation
\8\ Shipping label: Names, addresses, contact names and phone numbers of =
person shipping the package and
intended recipient (addressee).
\9\ DOT specifies that the shipper include an emergency response =
telephone number on the shipping documents (49
CFR 172.604).
[[Page 58025]]
II. Proposed Rule
This proposed rule would amend the existing regulations at 42 CFR=20
part 72 concerning the interstate shipment of infectious substances to=20
clarify and expand the existing requirements for proper packaging and=20
handling of these agents. The purpose of this regulation is to ensure=20
that all biological materials that contain, or may contain, an=20
infectious substance are packaged for interstate shipment in a manner=20
that minimizes the potential for leakage and possible contamination of=20
the environment, or direct physical contact with the contents by=20
persons handling such packages during transit. This rule will also=20
require that infectious agents and toxins capable of causing serious=20
infection, illness or death be labeled and tracked during shipment.
It also updates the requirements for facilities transferring or=20
receiving select agents, incorporating by reference the 4th edition of=20
the CDC/NIH publication Biosafety in Microbiological and Biomedical=20
Laboratories.
A. Definitions
Biological products--Biological product means a biological product=20
that is subject to preparation and manufacture in accordance with the=20
provisions of 9 CFR part 102 (Licensed Veterinary Biological Products),=20
9 CFR part 103 (Biological Products for Experimental Treatment of=20
Animals), 9 CFR part 104 (Imported Biological Products), 21 CFR part=20
312 (Investigational New Drug Application), or 21 CFR parts 600-680=20
(Biologics) and that, in accordance with such provisions, may be=20
shipped in interstate traffic. FDA-approved vaccines are exempt from=20
this regulation.
Only biological products that are known or presumed to contain an=20
infectious substance are subject to this regulation.
Clinical Specimens--A clinical specimen is any human or animal=20
material including, but not limited to excreta, secreta, blood and its=20
components, tissue and tissue fluids, that is collected for the purpose=20
of diagnosis, research, or other purposes. Formalin-fixed specimens are=20
excluded. Animal material clinical specimens are subject to the=20
regulation only if known or suspected of containing human pathogens.
Under the concept of Universal Precautions all bodily fluids of=20
human origin must be handled as if they are infectious in order to=20
minimize the potential for exposure to bloodborne pathogens. Section=20
72.3 in this NPRM meets those requirements.
Some clinical specimens are known or presumed to contain viable=20
infectious micro-organisms that could result in an infection if an=20
exposure occurred during a transport mishap. These specimens must be=20
packaged and labeled as infectious substances (see Sec. 72.4(a)). If=20
exposure could result in an extremely serious infection or illness in=20
an exposed worker or the public, such specimens are considered special=20
infectious substances and must be tracked during shipment.
Infectious substance--CDC has replaced the term ``etiologic agent''=20
with the DOT and international term ``infectious substance''. For=20
purposes of this regulation, an infectious substance is any substance,=20
clinical specimen or culture, isolate, or other derivative of a=20
clinical specimen that contains, or is suspected of containing a viable=20
infectious virus, prion, or a viable microorganism, such as a=20
bacterium, rickettsia, parasite or fungus, that is known or reasonably=20
believed to cause disease in humans. Toxins known to be pathogenic are=20
to be packaged and shipped either as infectious substances or as=20
special infectious substances (Sec. 72.5), as applicable.
Examples of infectious substances include:
1. All cultures containing or suspected of containing a=20
microorganism that causes or may cause disease in humans;
2. All human or animal clinical specimens that are known or=20
suspected of containing an infectious microorganism or toxin;
3. Environmental samples to the extent that they are suspected of=20
containing human pathogens at a level that presents risk of infection.
4. Other specimens not included above and designated as infectious=20
by a qualified person (e.g., physician, scientist, veterinarian,=20
nurse).
To maintain consistency with DOT regulations, a qualifying sentence=20
has been added to the definition of an infectious substance that states=20
that a microbial toxin that causes disease in humans will be packaged=20
and shipped as an infectious substance.
Packaging--A change in this NPRM is the adoption of DOT and IATA=20
terminology to clarify that there is agreement among the various=20
organizations involved in regulating this area. The terms ``primary=20
container'', ``secondary container'', and ``outer container'' have been=20
replaced with the DOT terms and definitions of ``primary receptacle'',=20
``secondary packaging'', and ``outer packaging'.
Special infectious substance means any of the microbiological=20
agents or toxins listed in Sec. 72.5 or appendix A to part 72 (proposed=20
to be recodified as appendix to subpart B). These special infectious=20
substances include those agents listed in the CDC/NIH publication,=20
Biosafety in Microbiological and Biomedical Laboratories, as biosafety=20
level (BSL) 4 and most of the BSL3 agents. Special infectious=20
substances present a potentially high risk of infection and/or death to=20
persons exposed to them through either direct contact, aerosol or=20
ingestion. Therefore, shipments of special infectious substances are=20
tracked to assure their safe arrival.
B. Transport of Clinical Specimens
Clinical specimens are to be packaged in such a manner that they=20
will remain intact under conditions that normally occur during transit.=20
If the primary receptacle were to break or leak during transit, the=20
specimen would be contained by the absorbent material and by the=20
secondary packaging, so no material would leak to the outside surface=20
of the outer packaging.
Packaging requirements for clinical specimens proposed in this NPRM=20
are similar to those for infectious substances, except that the=20
proposed performance standards are less rigorous. These packaging and=20
labeling requirements meet the specifications established by OSHA and=20
various international agencies.
C. Transport of Infectious Substances
Infectious substances are to be packaged in such a manner that they=20
would withstand conditions which would normally occur during transit=20
and would not leak even if the primary receptacle were to break. In=20
addition, the proposed packaging requirements have been enhanced by=20
adding a requirement that the packaging be capable of passing a drop=20
test. The completed package must be capable of passing the tests=20
specified in 49 CFR 178.609. The requirements established in this NPRM=20
meet those of DOT, OSHA, various international agencies and are=20
consistent with the 1999 IATA Dangerous Goods Regulations.
In keeping with DOT and the international guidelines and=20
regulations, volume/weight limits have been changed to four liters or=20
four kilograms in a single package (excluding the packaging and coolant=20
weights). An itemized list of contents must be enclosed between the=20
secondary packaging and outer packaging. The proposed rule also details=20
provisions associated with substances shipped refrigerated or frozen=20
(prefrozen packs,
[[Page 58026]]
wet or dry ice), shipped in liquid nitrogen, or as lyophilized=20
materials.
The proposed rule requires on the outer packaging a black and white=20
label bearing the words ``Infectious Substance'', CDC's telephone=20
number for reporting damaged packages, and the biohazard symbol. The=20
proposed rule also would require that the name, address, and telephone=20
number of both the shipper and recipient be affixed to the outer=20
package.
D. Transport of Special Infectious Substances; Failure to Receive
This proposed rule would be unique in requiring that the most=20
dangerous human pathogens be shipped as ``special infectious=20
substances''. These agents include those identified for work at=20
biosafety level 3 and 4 as specified in the Biosafety in=20
Microbiological and Biomedical Laboratories publication.
Special infectious substances must be shipped by the carrier and a=20
system that provides for tracking the shipment and notifying CDC if the=20
packages are not received. Information gathered by CDC from such=20
notifications will be useful in identifying problems and implementing=20
corrective actions.
E. Select Agents
Some of the microorganisms listed as special infectious substances=20
are also considered to be ``select agents'' and are regulated in 42 CFR=20
72.6 (Additional Requirements for Facilities Transferring or Receiving=20
Select Infectious Agents) (proposed here to be renumbered as Section=20
72.11). The only changes in this proposed rule to Sec. 72.6 are at=20
Sec. 72.6 (a)(5) (now Sec. 72.11 (a)(5)), and Sec. 72.6 (c)(1) (now=20
Sec. 72.11(c)(1)), which are revised to incorporate the 4th edition of=20
the CDC/NIH publication Biosafety in Microbiological and Biomedical=20
Laboratories.
F. Variations
To promote innovation and allow for new technologies, the proposed=20
rule would allow the Director, CDC, to approve variations from the=20
requirements of this subpart if, upon written application, it is found=20
that such variations provide protection at least equivalent to that=20
provided by the requirements in this subpart, as finalized, and such=20
findings are made a matter of official written record.
G. Penalties
Violations of the rule would be subject to criminal penalties as=20
prescribed in 42 U.S.C. 271 and 18 U.S.C. 3559, 3571. Specifically,=20
individuals in violation of the rule would be subject to a fine or=20
imprisonment of not more than one year, or both.
III. Procurement of Labels
Shippers will be able to order a supply of the two shipping labels=20
described in the regulations from private printers by furnishing them=20
the exact specifications provided in the final rule, or by purchasing=20
the labels from the Superintendent of Documents (U.S. Government=20
Printing Office, Mail Stop: SSOP, Washington, D.C. 20402-9328).
IV. Analysis of Impacts
A. Review Under Executive Order 12866, Sections 202 and 205 of the=20
Unfunded Mandate Reform Act of 1995 (P.L. 104-4), and by the Regulatory=20
Flexibility Act (5 U.S.C 603-605)
The Department has examined the potential impact of this proposed=20
rule as directed by Executive Order 12866, by sections 202 and 205 of=20
the Unfunded Mandate Reform Act of 1995 (Pub. L. 104-4), and by the=20
Regulatory Flexibility Act (5 U.S.C. 603-605).
Executive Order 12866 directs Federal agencies to assess the costs=20
and benefits of available regulatory alternatives, and, when regulation=20
is necessary, to select regulatory approaches that maximize net=20
benefits. This proposed rule is designed to ensure that all biological=20
materials that contain, or may contain, an infectious substance are=20
packaged in a manner for interstate shipment that minimizes the=20
potential for leakage and possible contamination of the environment or=20
direct physical contact with the contents by persons handling such=20
packages during transit. The proposed rule is designed to complement=20
other shipping requirements developed by the Departments of Commerce,=20
Agriculture, and Transportation, the USPS, OSHA, and the International=20
Air Transport Association and, thereby, to reduce the burden on=20
shippers while imposing minimal administrative costs, and to prevent=20
possible serious, harmful effects to public safety and health. (The=20
proposal has been reviewed by the Office of Management and Budget under=20
the terms of the Executive Order.)
The Unfunded Mandates Reform Act of 1995, in sections 202 and 205,=20
requires Federal agencies to prepare several analytic statements before=20
proposing a rule that may result in expenditures of $100 million by=20
State, local and tribal governments, or by the private sector in any=20
one year. Because a final rule resulting from this proposal would not=20
result in expenditures of this magnitude, such statements are not=20
necessary.
The Regulatory Flexibility Act requires Federal agencies to prepare=20
a regulatory flexibility analysis of the potential impact of the=20
proposed rule on small entities and permits agency heads to certify=20
that a proposed rule will not, if promulgated, have a significant=20
economic impact on a substantial number of small entities. CDC does not=20
know how many small entities will be impacted by this regulation, and=20
does not know what the economic impact on those small entities would=20
be. However, CDC believes that packaging requirements set forth in this=20
rule would not be an additional burden on shippers because this is an=20
amendment to existing PHS rules with which shippers must comply. In=20
addition, it will harmonize these rules with other existing regulations=20
that shippers must follow. CDC believes that this rule will lessen=20
confusion regarding proper packaging and shipping of infectious=20
materials and will bring HHS regulations into conformity with other=20
regulations. CDC is requesting information/comments on the number of=20
small entities that would be impacted by this NPRM, the economic burden=20
on those small entities and why the Secretary should not certify that=20
this rule will have no significant impact on small entities. CDC is=20
also requesting comments/recommendations on other possible less=20
burdensome approaches to ensuring that all infectious or potentially=20
infectious materials are packaged and shipped in a way that minimizes=20
risks to workers, the public and the environment.
These regulations will help to ensure that all shippers are aware=20
of and utilize appropriate packaging when shipping infectious=20
substances, thereby protecting the public health.
B. Review Under the Paperwork Reduction Act of 1995
The proposed rule contains information collection requirements that=20
are subject to review by the Office of Management and Budget (OMB)=20
under the Paperwork Reduction Act of 1995. The title, description and=20
respondent description of the information collection are shown below=20
with an estimate of the annual reporting burden. The estimate includes=20
the time for reviewing instructions, gathering and maintaining the=20
necessary data, and completing and reviewing the collection of=20
information. With respect to the following collection of information,=20
CDC invites comments on: (a) Whether the proposed collection of=20
information is necessary for the proper performance of CDC's public
[[Page 58027]]
health functions, including whether the information shall have=20
practical utility; (b) the accuracy of CDC's estimate of the burden of=20
the proposed collection of information including the validity of the=20
methodology and assumptions used; (c) ways to enhance the quality,=20
utility, and clarity of the information to be collected; and (d) ways=20
to minimize the burden of the collection of information on respondents,=20
including through the use of automatic collection techniques or other=20
forms of information technology.
Title: Packaging and Handling of Infectious Substances and Select=20
Agents.
Description: The CDC proposes to amend the regulations concerning=20
the interstate shipment of infectious substances in order to clarify=20
and expand requirements for proper packaging and handling of these=20
agents. The proposed rule would ensure that all biological materials=20
that may contain an infectious substance are packaged for interstate=20
shipment in a manner that minimizes the potential for leakage and=20
possible contamination of the environment or direct physical contact=20
with the contents by persons handling such packages during transport.=20
It also updates the requirements for facilities transferring or=20
receiving select agents, incorporating by reference the 4th edition of=20
the CDC/NIH publication Biosafety in Microbiological and Biomedical=20
Laboratories.
Anyone handling damaged or leaking packages of infectious=20
substances during interstate shipment must isolate the package, notify=20
the shipper and intended recipient immediately and notify CDC as soon=20
as feasible (1-800-232-0124). When notifying CDC, the caller should=20
provide a description of the condition of the package, the name,=20
address and telephone number of the shipper, and any other pertinent=20
information, so that information and assistance can be provided, as=20
necessary, regarding appropriate decontamination and disposal=20
procedures.
Persons who ship packages containing special infectious substances=20
must notify the addressee of the date of shipment, and the addressee=20
must confirm receipt by telephone or other electronic means. If the=20
shipper does not receive such confirmation within 3 days of anticipated=20
delivery, the shipper must then contact CDC within 24 hours to enable=20
the agency to determine whether a public health response is necessary.=20
Information gathered by CDC from such notifications will also be useful=20
in identifying problems and implementing corrective actions.
Description of Respondents: Government agencies, universities,=20
research institutions, laboratories, private companies and others that=20
ship or receive infectious substances, and government or commercial=20
carriers of infectious substances.
Estimated Annual Reporting =
Burden
-------------------------------------------------------------------------=
---------------------------------------
Frequency =
Total
CFR section Number of of =
annual Hours per Total hours
respondents reporting =
responses response
-------------------------------------------------------------------------=
---------------------------------------
72.4(b)........................................ 500 =
1x/yr 50 0.1 5
72.5(b)........................................ 200 =
10/yr 2,000 0.1 200
72.5(c)........................................ 200 =
10/yr 2,000 0.1 200
72.5(d)........................................ 20 =
1/yr 20 0.2 4
=
----------------------------------------------------------------
Total =
409
-------------------------------------------------------------------------=
---------------------------------------
Reporting or Disclosures: These estimates are an approximation of=20
the average time expected to be necessary for a collection of=20
information. They are based on past experiences of respondents=20
reporting such information to CDC. There are no capital costs or=20
operating and maintenance costs for the respondents associated with=20
this information collection.
The agency has submitted a copy of this proposed rule to OMB for=20
its review of this information collection. Interested persons are=20
requested to submit written comments regarding this information=20
collection, including suggestions for reducing the burden, to the=20
Office of Information and Regulatory Affairs, OMB, New Executive Office=20
Building, 725 17th Street, NW, Rm. 10235, Washington, DC 20503, Att.:=20
Desk Officer for CDC.
List of subjects in 42 CFR Part 72
Biologics, packaging and containers, Transportation.
Dated: March 12, 1999.
Jeffrey Koplan,
Director, Centers for Disease Control and Prevention.
Dated: May 31, 1999.
Donna E. Shalala,
Secretary, Department of Health and Human Services.
For the reasons stated in the preamble, it is proposed to amend 42=20
CFR Chapter I, part 72, as follows:
PART 72--PACKAGING AND HANDLING OF INFECTIOUS SUBSTANCES AND SELECT=20
AGENTS
1. The authority citation for Part 72 is revised to read as=20
follows:
Authority: 42 U.S.C. 216, 264, 271; 31 U.S.C. 9701; 18 U.S.C.=20
3559, 3571; 42 U.S.C. 262 note.
2. The heading of part 72 is revised to read as set forth above.
3. Sections 72.1-72.5 are revised.
4. 72.6 is redesignated as Sec. 72.11.
5. A new Sec. 72.6 is added.
6. A heading for subpart A is added and sections Secs. 72.1-72.6=20
are transferred to subpart A.
7. A heading for subpart B is added and redesignated section 72.11=20
is transferred to subpart B and amended by revising paragraphs (a)(5)=20
and (c)(1).
8. Section 72.7 is redesignated as section 72.21.
9. A heading for subpart C is added and redesignated section 72.21=20
is transferred to subpart C.
10. Appendix A to Part 72 is transferred to subpart B and the=20
heading is revised to read ``Appendix to Subpart B''.
The additions and revisions to part 72 read as follows:
Subpart A--Interstate Shipment of Biological Materials That Contain=20
or May Contain Infectious Substances
Sec. 72.1 Purpose.
The purpose of this regulation is to ensure that all materials that=20
contain or may contain an infectious substance are packaged for=20
interstate shipment in a manner that minimizes the potential for=20
leakage and possible contamination of the environment or direct=20
physical contact with the contents by persons handling such packages=20
during transit.
[[Page 58028]]
The rule also requires the tracking of shipments of special infectious=20
substances and requires registration of certain select agents. The=20
requirements of this subpart are in addition to and not in lieu of any=20
other packaging or other requirements for the transportation of=20
infectious substances in interstate traffic as prescribed by the US=20
Department of Transportation, the US Postal Service and other agencies=20
of the Federal Government.
Sec. 72.2 Definitions.
As used in this subpart:
Absorbent material means material that is capable of absorbing=20
liquids. It may be either particulate or non-particulate, but if=20
particulate, it shall be contained so it does not leak out of the=20
package.
Biological product means a biological product that is subject to=20
preparation and manufacture in accordance with the provisions of 9 CFR=20
part 102 (Licensed Veterinary Biological Products), 9 CFR part 103=20
(Biological Products for Experimental Treatment of Animals), 9 CFR part=20
104 (Imported Biological Products), 21 CFR part 312 (Investigational=20
New Drug Application), or 21 CFR parts 600-680 (Biologics) and that, in=20
accordance with such provisions, may be shipped in interstate traffic.=20
Only biological products that are known or presumed to contain an=20
infectious substance are subject to this regulation. FDA-approved=20
vaccines are exempt from this regulation.
Clinical specimen (diagnostic specimen) is any human or animal=20
material including, but not limited to excreta, secreta, blood and its=20
components, tissue and tissue fluids, that is collected for the=20
purposes of diagnosis, research, or other purposes. Formalin-fixed=20
specimens are exempt from this regulation. Animal material clinical=20
specimens are subject to this regulation only if known or suspected of=20
containing human pathogens. All human clinical specimens are covered.
Coolant material means material such as ice, dry ice, liquid=20
nitrogen, and gel packs, that is included in the package to cool the=20
contents.
Infectious substance (etiologic agent) and infectious material are=20
considered synonymous. An infectious substance is defined as a=20
substance containing or suspected of containing an infectious virus,=20
prion, or a viable microorganism, such as a bacterium, rickettsia,=20
parasite or fungus, that is known or reasonably believed to cause=20
disease in humans. Toxins known to be pathogenic to humans are to be=20
packaged and shipped as infectious substances or special infectious=20
substances (Sec. 72.5). The term ``infectious substance'' excludes any=20
medical waste that is regulated under other federal regulations. For=20
purposes of this regulation, infectious substances include:
(1) All cultures containing or suspected of containing a=20
microorganism that causes or may cause disease in humans;
(2) All human or animal clinical specimens that are known or=20
suspected of containing an infectious microorganism or toxin;
(3) Environmental samples if they are suspected of containing human=20
pathogens at a level that presents risk of infection.
(4) Other specimens not included above and designated as infectious=20
by a qualified person (e.g., a physician, scientist, veterinarian,=20
nurse).
Interstate traffic means the movement, including any portion=20
entirely within a State or possession, from a point of origin in any=20
State or possession or from outside the Untied States, to a point of=20
destination in any other State or possession; or form any State or=20
possession to another country; or between a point of origin and a point=20
of destination in the same State or possession but through any other=20
State, possession or contiguous foreign country.
Outer packaging means the container in which a primary receptacle=20
and secondary package, together with any absorbent materials and=20
cushioning, is shipped.
Primary receptacle means a tube, vial, bottle, ampule, or similar=20
item that contains the material being shipped.
Secondary packaging means a container into which the primary=20
receptacle is placed.
Special infectious substance means any of the microbiological=20
agents or toxins listed in Sec. 72.5 or appendix to subpart B of this=20
part, including any human or animal specimens known or suspected of=20
containing such a microbial agent, or any other microorganism that=20
could cause serious infection and/or death in persons exposed to them=20
through either direct contact, aerosol or ingestion. Additional changes=20
to this list may be made through publication of a notice in the Federal=20
Register.
Sec. 72.3 Transportation of clinical specimens; minimum packaging=20
requirements.
(a) General requirements. No person may knowingly transport or=20
cause to be transported in interstate traffic, directly or indirectly,=20
any clinical specimen unless such material is packaged, labeled, and=20
shipped in accordance with the requirements of this section. However,=20
any clinical specimens known or suspected to contain an infectious=20
substance shall be labeled and packaged as described under Sec. 72.4.
(1) Clinical specimens shall be packaged to withstand conditions=20
incident to ordinary handling in transit, including shocks and pressure=20
changes, so that if leakage of the primary receptacle(s) occurs during=20
transit, the contents will be contained within the outer packaging.=20
Required packaging and components are as follows:
(i) A watertight primary receptacle.
(ii) Watertight secondary packaging.
(iii) Absorbent material must be placed between the primary=20
receptacle(s) and the secondary packaging. If multiple primary=20
receptacles are placed in a secondary packaging, they msut be placed so=20
as to ensure that contact between them is prevented. The absorbent=20
material must be sufficient to absorb the entire contents of all=20
primary receptacles.
(iv) Outer packaging must be of adequate strength for its capacity,=20
mass and intended use. Any package with liquid contents shall have=20
sturdy outer packaging constructed of corrugated cardboard, fiberboard,=20
wood, metal, or rigid plastic. Styrofoam, plastic bags and paper=20
envelopes are unacceptable outer packaging for such packages.
(2) The size of the outer package must be at least 100 mm (3.9=20
inches) in the smallest overall external dimension.
(3) The primary receptacle and the secondary packaging must be=20
capable of withstanding, without leakage, an internal pressure which=20
produces a pressure differential of not less than 95kPA (0.95 bar,=20
13.8lb/in2) in the temperature range of -40 deg. C to=20
+55 deg. C (-40 deg. F to 131 deg. F).
(4) An itemized list of contents must be enclosed between the=20
secondary packaging and the outer packaging.
(5) For substances shipped at ambient temperatures or higher, means=20
of ensuring a leak-proof seal of the primary receptacle, such as a heat=20
seal, skirted stopper or metal crimp seal must be provided. Screw caps=20
must be reinforced to ensure they do not leak. Evacuated specimen=20
collection tubes such as Vacutainer=7F (Becton-Dickinson,=20
Franklin Lakes, NJ) tubes do not require additional sealing.
(6) For substances shipped refrigerated or frozen (wet ice,=20
prefrozen packs, dry ice), ice or dry ice must be placed outside the=20
secondary packaging(s). Interior support must be provided to secure the=20
secondary packaging(s) in the original position as the ice or dry ice=20
melts or sublimates, respectively. If ice is used, the outer
[[Page 58029]]
packaging must be leak-proof. If dry ice is used, the outer packaging=20
must permit the release of carbon dioxide gas. The primary receptacle=20
must maintain its containment integrity at the temperature of the=20
refrigerant as well as the temperatures and pressure of air transport=20
to which the receptacle could be subjected if refrigeration were to be=20
lost.
(7) For substances shipped in liquid nitrogen, a watertight=20
material, capable of withstanding cryogenic temperatures must be used=20
as the primary receptacles. Secondary packaging must also withstand=20
very low temperatures. All requirements for shipment of liquid nitrogen=20
must also be observed. The primary receptacle must maintain its=20
containment integrity at the temperature of the refrigerant as well as=20
at the temperatures and pressure of air transport to which the=20
receptacle could be subjected if refrigeration were to be lost.
(8) For lyophilized substances, primary receptacles capable of=20
containing lyophilized substances must be used (including, but not=20
limited to, flame-sealed glass ampules or rubber-stoppered glass vials=20
with metal seals).
(9) The completed package must be capable of withstanding at least=20
a 1.2 meter drop on a hard unyielding surface without release of its=20
contents.
(10)(i) Biohazard Labeling is required for the primary receptacle=20
and outer packaging as described in 29 CFR 1910.1030, Occupational=20
Exposure to Bloodborne Pathogens.
(ii) The outer packaging shall bear a label as illustrated and=20
described below:
[GRAPHIC] [TIFF OMITTED] TP28OC99.052
(A) The color of material on which the label is printed shall be=20
bright orange; the printing shall be black. The color of the biohazard=20
symbol shall be black.
(B) The label shall be a rectangle measuring 51 mm (2 inches) high=20
by 102.5 mm (4 inches) long.
(C) The biohazard symbol, measuring 40 mm (1.56 inches) in=20
diameter, shall be centered on a square measuring 51 mm (2 inches) on=20
each side.
(D) Size of the letters (Helvetica) on the label shall be as=20
follows:
Biohazard--16 pt.
Clinical specimens--14 pt.
Packaged in compliance with 42 CFR part 72--6 pt.
In case of damage or leakage, notify--10 pt.
Shipper and Receiver--10 pt.
(iii) The outer packaging shall also bear a shipping label with the=20
names, addresses, and contact names and telephone numbers of the=20
individual/institution sending the package and the intended recipient=20
(addressee).
(b) Leaking packages. The carrier, the receiver, or anyone handling=20
a package described in paragraph (a) of this section that is leaking,=20
shall upon discovery of leakage, isolate the package, and immediately,=20
or as soon as feasible, notify the shipper and intended recipient to=20
receive instructions on clean-up and disposition of the package.
Sec. 72.4 Transportation of infectious substances; minimum packaging=20
requirements.
(a) General requirements. No person may knowingly transport or=20
cause to be transported in interstate traffic, directly or indirectly,=20
any infectious substance, including clinical specimens or biological=20
products that are known or presumed to contain infectious substances,=20
unless such material is packaged, labeled, and shipped in accordance=20
with the requirements of this section.
(1) Infectious substances shall be packaged to meet the=20
requirements of Sec. 72.3(a) (1)-(8).
(2) The maximum amount of infectious substances that may be placed=20
in a single outer shipping package shall not exceed four liters or four=20
kilograms, excluding the packaging and coolant weights.
(3) In addition, each complete package must be capable of passing=20
the tests specified in 49 CFR 178.609.
(4)(i) Biohazard Labeling is required for the primary receptacle=20
and outer package as described in 29 CFR 1910.1030, Occupational=20
Exposure to Bloodborne Pathogens.
(ii) The outer packaging shall bear a label as illustrated and=20
described below:
[[Page 58030]]
[GRAPHIC] [TIFF OMITTED] TP28OC99.053
(A) The color of material on which the label is printed shall be=20
white and the printing shall be in black; the biohazard symbol shall be=20
in black.
(B) The label shall be a diamond-on-point measuring, at a minimum,=20
51 mm (4 inches) on each side.
(C) The black biohazard symbol, measuring 21 mm (.81 inches) in=20
diameter, shall be centered on a square measuring 51 mm (2 inches) on=20
each side.
(D) Size of the letters (Helvetica) on the label shall be as=20
follows:
Infectious Substance--16 pt.
Packaged in compliance with 42 CFR Part 72--5 pt.
In case of damage or leakage--7 pt.
Immediately notify--7 pt.
Public Health Authority--7 pt.
In U.S.A.--5 pt
Centers for Disease Control and Prevention--5 pt.
Atlanta, GA--5 pt
1-800-232-0124--5 pt.
6--24 pt.
(E) The number 6 (mandated by the DOT) shall be centered at the=20
bottom of the label.
(iii) The outer packaging and the secondary packaging shall also=20
bear labels with the names, addresses, and contact names and telephone=20
numbers of the individual/institution sending the package and of the=20
intended recipient (addressee).
(b) Damaged or leaking packages. The carrier, the receiver, or=20
anyone handling a package described in paragraph (a) of this section=20
that is damaged or leaking, shall upon discovery of damage or leakage,=20
isolate the package and immediately, or as soon as feasible, in order=20
to receive instructions on appropriate decontamination and disposal,=20
notify the shipper, receiver, and the Centers for Disease Control and=20
Prevention by telephone at 1-800-232-0124. The caller shall provide a=20
description of the condition of the package; the name, address and=20
telephone number of the shipper; and other pertinent information.
(This information collection has been approved by OMB (0920-0199)).
Sec. 72.5 Packaging and method of shipment of special infectious=20
substances; failure to receive.
(a) List of special infectious substances. (1) The following=20
microorganisms and toxins are considered special infectious substances=20
because they present a potentially high risk of infection and/or death=20
to persons exposed to them through either direct contact, aerosol or=20
ingestion. Shipments of special infectious substances must be tracked=20
to assure their safe arrival.
Bacterial Agents
Bacillus anthracis
Bartonella bacilliformis
Brucella, all species
Burkholderia (Pseudomonas) mallei
Burkholderia (Pseudomonas) pseudomallei
Clostridium botulinum
Francisella tularensis
Mycobacterium tuberculosis (drug-resistant strains)
Yersinia pestis
Viral and Rickettsial Agents
Arboviruses assigned to Biosafety level 3 or 4 in the CDC/NIH=20
publication Biosafety in Microbiological and Biomedical=20
Laboratories, which may be purchased from the Superintendent of=20
Documents, U.S. Government Printing Office, Washington, D.C. 20402.
Other Viral Agents
Crimean-Congo hemorrhagic fever virus
Eastern Equine Encephalitis virus
Ebola virus
Hantaan virus (Korean hemorrhagic fever virus)
Hantavirus (all viruses of genus)
Herpesvirus simiae (B virus)
Lassa fever virus
Lymphocytic choriomeningitis virus
Marburg virus
Pox viruses pathogenic for humans (e.g., smallpox, monkeypox)
South American Hemorrhagic fever viruses (Junin, Machupo, Sabia,=20
Flexal, Guanarito)
Tick-borne Encephalitis complex viruses
Venezuelan Equine Encephalitis virus
Yellow fever virus
Rickettsial Agents:
Rickettsia rickettsiae
Rickettsia prowazekii
Coxiella burnetti
Fungal Agents
Coccidioides immitis
Histoplasma capsulatum
Histoplasma duboisii
Toxins
Toxins listed in appendix to Subpart B of this part are to be=20
shipped as special infectious substances. Other microbial toxins=20
known to be pathogenic shall be shipped as infectious substances, as=20
provided under Sec. 72.4.
(2) This list may be supplemented through publication of a notice=20
in the Federal Register. Call 1-888-232-3299 (the FAX Information=20
system in CDC's Office of Health and Safety) for a copy of the current=20
list, or check the CDC website at .
(b) Packaging and method of shipment. All materials that contain or=20
are reasonably believed to contain a special infectious substance shall=20
be packaged and labeled for interstate shipment according to the=20
requirements of Sec. 72.4. In addition, the shipper shall: Use a=20
shipping system that provides for tracking during transport (e.g.,=20
registered mail or those of certain
[[Page 58031]]
private carriers); Provide 24 hours-per-day telephonic response to=20
emergency calls from carriers in case of a spill or incident involving=20
a package containing a special infectious agent; and, Notify the=20
addressee by telephone or other electronic means of the date of=20
shipment on the date of shipment, or provide a written schedule of=20
shipment in advance, and request confirmation of receipt of each=20
shipment. Records of such notifications shall be retained by the=20
shipper until notified of receipt.
(c) Confirmation of receipt. Upon receipt, the addressee shall=20
provide confirmation to the shipper by telephone or other electronic=20
means.
(d) Failure to receive. When confirmation of receipt of material=20
designated in paragraph (a) of this section is not received by the=20
shipper within 3 days following anticipated delivery of the package,=20
the shipper shall notify the carrier which shall immediately seek to=20
ascertain the disposition of the package. In addition, the shipper=20
shall notify the Centers for Disease Control and Prevention within 24=20
hours by telephone at 1-800-232-0214 to enable the agency to determine=20
whether a public health response is necessary.
Sec. 72.6 Requirements; variations.
The Director, Centers for Disease Control and Prevention, may=20
approve variations from the requirements of this subpart if, upon=20
written application, review and evaluation, it is found that such=20
variations provide protection at least equivalent to that provided by=20
compliance with the requirements specified in this subpart, and such=20
findings are made a matter of official written record.
Sec. 72.7 [Redesignated as Sec. 72.21]
Subpart B--Handling of Select Agents
Sec. 72.11 Additional requirements for facilities transferring or=20
receiving select agents.
(a) * * *
(5) The requirements for BSL-2, 3, and 4 operations pertaining to=20
this section are contained in the CDC/NIH publication, ``Biosafety in=20
Microbiological and Biomedical Laboratories,'' Fourth Edition, May 1999=20
which is hereby incorporated by reference. The Director of the Federal=20
Register has approved under 5 U.S.C. 552(a) and 1 CFR part 51 the=20
incorporation by reference of the above publication. Copies may be=20
obtained from the Superintendent of Documents, U.S. Government Printing=20
Office, Washington, DC 20402. Copies may be inspected at the Centers=20
for Disease Control and Prevention, 1600 Clifton Road, Atlanta,=20
Georgia, or at the Office of the Federal Register, 800 North Capitol=20
Street NW, Suite 700, Washington, DC. The manual is also available on=20
the CDC web site at od/ohs/biosfty/bmbl4/bmbl4toc.htm.
* * * * *
(c) * * *. (1) The Secretary may authorize a state agency or=20
private entity to register facilities under paragraph (a) of this=20
section, if the Secretary determines that the registering entity's=20
criteria for determining the biosafety standards for facilities=20
handling select agents are consistent with the requirements contained=20
in the CDC/NIH publication ``Biosafety in Microbiological and=20
Biomedical Laboratories,'' Fourth Edition.
* * * * *
Subpart C--Penalties
Sec. 72.21 [Redesignated from Sec. 72.7]
Appendix A to Part 72 [Transferred to Subpart B and heading=20
revised]
Appendix to Subpart B
* * * * *
[FR Doc. 99-27640 Filed 10-27-99; 8:45 am]
BILLING CODE 4163-18-P
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Date: Wed, 3 Nov 1999 09:00:18 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: New
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Hi, Carol -
Welcome to the Wonderful World of Biosafety! I'm sure my cohorts in the
field will have many suggestions to help you get underway so I'll weigh in
with just one and let them add to the list as they will.
You haven't said what type of research your institute will be involved in
but the fact that it feels it's important to have a Biosfety Officer would
lead me to believe there will be a strong component of biological/biomedical
research. If that assumption is valid, let me suggest that, while your CIH
and CSP certifications will be helpful in many ways, they cannot substitute
for a strong background in microbiology and infectious diseases. This is
really the heart of our profession. If you already have a degree in micro,
you'll be in pretty good shape to jump right into your job as BSO. If not,
I strongly encourage you to begin to acquire current microbiological
knowledge as quickly as possible. If you're near a medical school or
university, consider taking a full-blown course (or probably series of
courses) leading to and throgh pathogenic microbiology. Spend extra time
learning about viruses as these agents are not only important in public
health (they are the major drivers of the Bloodborne Pathogens Standard!),
they also contain the only Risk Group 4 agents and a good chunk of the RG3s,
and are common tools in rDNA research. The importance of microbiology to
our field can be seen in the fact that the CBSP certification exam is
administered through the National Academy of Microbiology.
That's my two-bits worth. OK, fellow Biosaftyers, jump on in! Again,
welcome, Carol. You'll find most members of this profession to be
intelligent, witty, friendly, highly ethical and extremely helpful. Join
ABSA and get to know us - I think you'll like what you see.
-- Glenn
------------------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
University of California, San Francisco
Voice 415-476-2097
Fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Petty, Carol [mailto:cpetty@]
Sent: Wednesday, November 03, 1999 8:29 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: New
I just recently joined this email group and would like to introduce myself.
I am Carol Petty and my background is industrial hygiene and safety. I am
now becoming a biosafety officer for a research facility in New Mexico. I
would welcome any suggestions or comments from those who have been in this
field for awhile in the best way to set up the program and also the medical
surveillance. For example, are there any good references on the vaccines
available for agents. I am using the CDC Biosafety in Microbiological and
Biomedical Laboratories primarily at the moment. Thanks.
Carol L. Petty, C.I.H., C.S.P.
Industrial Hygienist
Phone: (505) 845-1076
Fax: (505) 845-1174
email: cpetty@
=========================================================================
Date: Wed, 3 Nov 1999 09:25:54 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: Media disposal
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Hi, Robin -
At UCSF, we encourage our staff to sewer all liquid and semi-liquid lab
waste. If it's infectious, it must be autoclaved or (in accordance with a
recent change to the California Medical Waste Management Act) treated with a
chemical disinfectant/sterilant recognized by NIH, CDC or ABSA before
sewering. This would appear to be the easiest approach and I agree that
plenty of water must be used when sewering molten agar - I can imagine a
pretty fine agar plug otherwise!
-- Glenn
------------------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
University of California, San Francisco
Voice 415-476-2097
Fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Robin Newberry [mailto:wnewber@CLEMSON.EDU]
Sent: Tuesday, November 02, 1999 12:41 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Media disposal
We've got a small problem with media disposal and I was wondering if anyone
else had addressed this issue. I've a researcher who is having his employees
pour used, autoclaved media straight into a dumpster. The media consists of:
LB plates: 1% Tryptone, 0.5% Yeast extract, 1% NaCl, 1.5% Agar
and
LB medium: 1% Tryptone, 0.5% Yeast extract, 1% NaCl
Before use they add chloramphenicol (stock solution: 20 mg/ml in ethanol) at
below 70 degrees C after autoclaving. (The chloramphenicol should be
deactivated when they autoclave it after use.)
It's harmless, but it stinks (like hot media does) and we get many, many
complaints - some from others in this building (not a bio building) and the
folks who empty the dumpster (our own facilities employees). He's been doing
this elsewhere on campus without problems (so he says), but has opened a new
operation in a different building.
So far I've suggested that he:
pour the media into 5 gallon buckets and seal the lid prior to disposal in
the dumpster (he is unwilling to do this);
Pour the hot media down the drain with copious amounts of hot water (turns
out they toss all sorts of things in when autoclaving - gloves, etc. - so
it's not just media. Even copious amounts of hot water won't wash a glove
down the drain);
and that they autoclave in autoclave bags and place them in the dumpster
(gently). (No answer so far on this one).
I realize that we're asking him to alter his procedures for disposing of a
non-hazardous material merely to accommodate a few people who find the odor
offensive, but I find his reluctance to be a good neighbor frustrating.
Any suggestions?
Robin
W. Robert Newberry, IV CIH, CHMM
Director, Environmental Health and Safety
Clemson University
wnewber@clemson.edu ehs@clemson.edu
=========================================================================
Date: Wed, 3 Nov 1999 11:42:23 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: FW: Testing for Replication Competent Retrovirus
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
For those of you who need to keep up with such things,
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
Phone:=A0 303-315-6754
Pager: 303-266-5402
Fax:=A0=A0=A0=A0=A0 303-315-8026
-----Original Message-----
From: owner-cberinfo@archie.
[mailto:owner-cberinfo@archie.]=20
Sent: Wednesday, November 03, 1999 9:50 AM
To: cberinfo@archie.
Subject: Testing for Replication Competent Retrovirus
************************************************************
This document is available on CBER's FAX Information System
as document number 0771. Access the FAX System by calling
1-888-CBER-FAX (within the U.S.) or 301-827-3844 (outside
the U.S. and local to Rockville, MD).
=20
This document is also available on the internet at
************************************************************
Guidance for Industry
Supplemental Guidance on Testing for Replication Competent Retrovirus
in Retroviral Vector Based Gene Therapy Products and During Follow-up
of Patients in Clinical Trials Using Retroviral Vectors
DRAFT GUIDANCE
This guidance document is being distributed for comment purposes only.
Comments and suggestions regarding this draft document should be
submitted within 90 days of publication in the Federal Register
notice announcing the availability of the draft guidance. Submit
comments to Dockets Management Branch (HFA-305), Food and Drug
Administration, 5630 Fishers Lane, rm. 1061, Rockville, MD 20852.
All comments should be identified with the docket number
listed in the notice of availability that publishes in the Federal
Register.
Additional copies of this draft guidance document are available from
the Office of Communication, Training and Manufacturers Assistance
(HFM-40), 1401 Rockville Pike, Rockville, MD 20852-1448, or by
calling 1-800-835-4709 or 301-827-1800, or from the Internet at
For questions on the content of this draft document contact
Carolyn Wilson, Ph.D., 301-827-0481.
U.S. Department of Health and Human Services
Food and Drug Administration
Center for Biologics Evaluation and Research (CBER)
November 1999
________________________________________________________________
Draft - Not for Implementation
________________________________________________________________
This draft guidance document represents the agency's current thinking
regarding testing for replication competent retrovirus in retroviral
vector based gene therapy products. It does not create or confer any
rights for or on any person and does not operate to bind FDA or the
public. An alternative approach may be used if such approach
satisfies the requirements of the applicable statute, regulations, or
both.
________________________________________________________________
TABLE OF CONTENTS
I. INTRODUCTION
II. BACKGROUND
III. RECOMMENDATIONS FOR PRODUCT TESTING
A. When to Test
B. Amounts for Testing
IV. RECOMMENDATIONS FOR PATIENT MONITORING
A. Testing Schedule
B. Recommended Assays
V. DOCUMENTATION OF RCR TESTING RESULTS
VI. CONCLUSION
VII. REFERENCES
APPENDIX
_________________________________________________________________
GUIDANCE FOR INDUSTRY
Supplemental Guidance on Testing for Replication Competent Retrovirus
in Retroviral Vector Based Gene Therapy Products and During Follow-up
of Patients in Clinical Trials Using Retroviral Vectors
I. INTRODUCTION
This guidance document applies to the manufacture of gene therapy
retroviral vector products intended for in vivo or ex vivo use
and to follow-up monitoring of patients who have received
retroviral vector products. Guidance is provided for replication
competent retrovirus (RCR) testing during manufacture, including
timing, amount of material to be tested, and general testing
methods. In addition, guidance is provided on monitoring
patients for evidence of retroviral infection. This guidance
document is intended to supplement the guidance and
recommendations pertaining to RCR testing given in the following
documents: 1) "Guidance for Industry: Guidance for Human Somatic
Cell Therapy and Gene Therapy," March 1998; and 2) a letter to
Sponsors of INDs Using Retroviral Vectors, dated September 20,
1993. For general guidance on gene therapy refer to "Guidance
for Industry: Guidance for Human Somatic Cell Therapy and Gene
Therapy" March 1998.
II. BACKGROUND
CBER's current recommendations for RCR testing during retroviral
vector production and patient monitoring were developed in 1993,
at a time when clinical experience with retroviral vectors was
limited (4). The overriding safety issues associated with the
use of retroviral vectors are exemplified by the findings of an
experiment involving administration of ex vivo transduced bone
marrow progenitor cells that had been inadvertently exposed to
high titer RCR contained in the retroviral vector material to
severely immunosuppressed Rhesus monkeys. In this setting, 3/10
animals developed lymphomas and died within 200 days (3). The
RCR was presumed to be etiologically associated with the disease
by virtue of the presence of multiple murine RCR sequences in
the monkey lymphomas and the observed correlation between the
lack of antiretroviral antibody response and the development of
prolonged retroviremia and disease (9, 11). Since 1993,
accumulating experience with different vector producing cells,
RCR detection assays and results from patient monitoring have
allowed the generation of a small data base of information on
the safety of the use of retroviral vectors in clinical
applications of gene therapy. This information base has provided
a framework for discussion of the RCR recommendations by Center
for Biologics Evaluation and Research and the gene therapy
community. Public discussion and development of these
supplemental recommendations have taken place during the
Retroviral Breakout Sessions at the 1996 and 1997 FDA/NIH Gene
Therapy Conferences, with representatives of the gene therapy
community, and through the publication of the FDA considerations
on these issues (12).
III. RECOMMENDATIONS FOR PRODUCT TESTING
A. When to Test
RCR may develop at any step during manufacturing from
development of the initial master cell bank through production
of the retroviral vector supernatant. In addition, the growth
of ex vivo transduced cells provides the potential for
amplification of any RCR contaminant which may be below the
level of detection in the retroviral vector supernatant.
Therefore, current testing recommendations include testing of
material from multiple stages of product manufacture (see
Table 1). Use of a cell bank system is recommended in order
to ensure an adequate and consistent supply of vector
producer cells (VPC). The Master Cell Bank (MCB)is a
collection of cells of uniform composition derived from a
single tissue or cell. The Working Cell Bank (WCB) is derived
from one or more ampules of the MCB, expanded by serial
subculture to a specified passage number (refer to Points to
Consider in the Characterization of Cell Lines Used to
Produce Biologicals, 1993).
1. Testing of Vector Producer Cell Master Cell Bank (one time
testing)
Both VPC and supernatant from production of a MCB should
be tested for RCR using a cell line permissive for the
RCR most likely to be generated in a given producer cell
line. For example, VPC containing amphotropic Murine
Leukemia Virus (MLV) envelope should be tested for RCR on
a cell line such as Mus dunni that is permissive to
infection by amphotropic MLV-like RCR, while VPC
containing the gibbon ape leukemia virus envelope should
be tested on a human cell line. Other retroviral =
envelopes
should be tested on a cell line permissive for infection
by the relevant RCR.
If derivation of VPC includes use of a retroviral vector
containing an envelope distinct from the packaging vector,
for example, an ecotropic MLV, the potential exists for
introduction of an RCR with that envelope. Even though
an ecotropic MLV RCR may present a minimal direct safety
risk to humans, the presence of any replication-competent
genome in the VPC is problematic because of the increased
probability of generating an RCR with a human host range
by recombination with elements within the VPC.
In those cases where VPC are derived at any step by
infection with an ecotropic retroviral vector, testing of
the MCB for the presence of ecotropic RCR is recommended.
Both cells and supernatants should be tested using a
method validated to detect the appropriate positive
control (for example: D56 (2) or XC (10)). Refer to the
guidance provided in section III.B. to determine the
amount of material for testing.
2. Working Cell Bank Testing (one time testing)
Either supernatant testing or cocultivation of cells for
RCR is recommended using conditions described for master
cell bank testing.
3. Testing of Retroviral Vector Supernatant Product and End
of Production Cells
Both retroviral vector supernatant lots and end of
production cells should be tested for RCR as specified
in section III.B. This recommendation is based on data
and experience reported at the 1997 FDA/NIH Gene Therapy
Conference in which RCR in vector production lots was not
consistently detected by both assays or one assay to the
exclusion of the other. These data support the position
that dual testing provides a complementary approach to
assuring RCR free retroviral supernatant.
4. Testing of Ex Vivo Transduced Cells
a. Cultured < 4 days after transduction
Data presented at the 1997 FDA/NIH Gene Therapy
Conference indicated that for ex vivo transduced cells,
a minimum culture period of 4 days from the start of
transduction is necessary for amplification and
detection of an RCR. As a result, for ex vivo
transduced cells cultured for a period less than four
days, archiving of the quantity of product needed to
perform RCR testing is recommended in place of active
RCR testing. Refer to the guidance in section III.B.
to determine the amount of material to be archived.
Samples should be archived with appropriate safeguards
to ensure long-term storage (e.g., a monitored freezer
alarm storage system) and an efficient system for the
prompt linkage and retrieval of the stored samples with
the medical records of the patient and the production
lot records.
b. Cultured > 4 days
When ex vivo transduced cells are in culture for a
period of time greater than or equal to 4 days from the
start of transduction, cells and the appropriate volume
of culture supernatant should be tested for RCR. Refer
to guidance in section III.B. to determine amount of
material for testing. In situations where ex vivo
transduced cells cannot be cryopreserved during =
testing,
and must be administered to patients prior to the
availability of testing results, culture assays should
be initiated at the time of patient administration.
In these situations, alternative methods such as PCR
may be appropriate to provide an initial analysis. Any
alternative methods should be developed in consultation
with CBER. Data on sensitivity, specificity and
reproducibility will be needed to support the use of
alternative methods.
_____________________________________________________________________
Table 1. Recommendations for Product Testing
RCR Testing
RCR Testing for for =
Ecotropic=20
Expected RCR (a) MLV
=20
Manufacturing Step Cells Supernatant Cells
Supernatant
MCB
-Derived by infection with Yes Yes Yes Yes =
Ecotropic MLV vector
-Derived by transfection Yes Yes No No
of retroviral vector plasmid
WCB Yes OR Yes No No
End of Production Cells Yes NA (b) No No
Vector-Containing Supernatant NA Yes No No
Ex vivo Transduced Cells
-Cultured According to our state regulations we can treat, destroy and dispose
> regulated medical waste on-site or off-site.
>
>
> Do any of you treat and destroy Regulated Medical Waste on-site?
> I would like information on waste other than sharps, liquids(including human
> blood), human and animal pathological wastes.
>
> Specifically, I would like information on Cultures and Stocks oof Infectious
> materials including HIV.
>
> The acceptable technologies (according to our State Regs) include:
>
> 1. Incineration
> 2. Chemical disinfection
> OR
> 3. Steam sterilisation followed by grinding and shredding.
>
> Do any of you have grinders or shredders?
> Is it cost effective to do that on-site as opposed to autoclaving and sending
> off-site for destruction?
>
> As always, thanks a lot for your input.
>
>
>
>
>
>
>
> Ninni Jacob, CHP
> Radiation and Biological Safety Officer
> Office of Risk Management
> Brown University - Box 1914
> 164 Angell Street
> Providence, RI 02912
>
> Tel:401 863 1738
> Fax:401 863 7676
>
> email: Ninni_Jacob@brown.edu
J. Paul Jennette, P.E.
Biosafety Engineer
Cornell University
College of Veterinary Medicine
Biosafety Program
S3-010 Schurman Hall, Box 38 (607) 253-4227
Ithaca, New York 14853-6401 fax -3723
--=====================_4669834==_.ALT
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Ninni,
We also have the option of both on and off-site treatment for RMW.
On-site treatment at non-hospital facilities requires a permit from our
state Department of Environmental Conservation, and getting the permit is
a complex and expensive process. Our state regs require extensive
testing and reporting on an ongoing basis, as well.
"Cost effective" also depends largely on the quantity of RMW
you must handle. Are you looking at treating waste from the whole
University? If so, it could very well be cost-effective to treat
the waste onsite, in a centralized facility. At that scale, steam
sterilization systems such as those manufactured by Hydroclave or Tempico
(Rotoclave) are often used, and they are typically integrated with
grinders or shredders. These are big systems, that are heavily
automated, and minimize waste handling through the use of
conveyors. For smaller quantities, shipping waste offsite may be
preferable from the perspectives of cost, headache, and liability.
We currently operate a permitted, onsite incinerator for our pathological
waste and ship our RMW offsite. We are looking into non-burn
alternatives for treating both our path waste and RMW onsite.
Unfortunately the term "grinder" is alot like "car"
-- some work well and some don't. We had a small hammermill grinder
a few years ago that was problematic, but I've seen other grinders that
work great.
I hope this helps.
Cheers - Paul
At 04:57 PM 11/2/99 -0500, you wrote:
According to our state regulations we can
treat, destroy and dispose regulated medical waste on-site or off-site.
Do any of you treat and destroy Regulated Medical Waste on-site?
I would like information on waste other than sharps,
liquids(including human blood), human and animal pathological wastes.
Specifically, I would like information on Cultures and Stocks oof
Infectious materials including HIV.
The acceptable technologies (according to our State Regs) include:
1. Incineration
2. Chemical disinfection
OR
3. Steam sterilisation followed by grinding and shredding.
Do any of you have grinders or shredders?
Is it cost effective to do that on-site as opposed to autoclaving and
sending off-site for destruction?
As always, thanks a lot for your input.
Ninni Jacob, CHP
Radiation and Biological Safety Officer
Office of Risk Management
Brown University - Box 1914
164 Angell Street
Providence, RI 02912
Tel:401 863 1738
Fax:401 863 7676
email: Ninni_Jacob@brown.edu
J. Paul Jennette, P.E.
Biosafety Engineer
Cornell University
College of Veterinary Medicine
Biosafety Program
S3-010 Schurman Hall, Box
38 (607)
253-4227
Ithaca, New York
14853-6401 fax
-3723
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=========================================================================
Date: Thu, 4 Nov 1999 09:13:37 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: job posting
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UMDNJ has a job opening near Philadelphia. Biosafety people should =
respond to Human Resources as indicated, but if you have questions, you =
could email Lindsey Kayman at kayman@umdnj.edu. =20
LABORATORY SAFETY SPECIALIST
UMDNJ, the University of Medicine & Dentistry of New Jersey, the =
nation's largest comprehensive Health Sciences University, is seeking a =
Laboratory Safety Specialist for the Stratford Campus, 25 minutes from =
downtown Philadelphia.=20
The salary range is from $43,269.66 - $60,172.99. Reporting to the =
Campus Safety Manager, this individual will identify, evaluate, =
eliminate and/or minimize occupational and environmental hazards in =
laboratories and related premises.=20
In addition to a Master's degree in industrial hygiene, environmental or =
occupational health sciences, chemistry or a related technical =
discipline, a minimum of three (3) years of professional work experience =
in the field of industrial hygiene, laboratory safety, or =
occupational/environmental health prevention programs is required. A =
Bachelor's degree and five years of experience in laboratory safety, =
industrial hygiene, biosafety or occupational/environmental safety and =
health is also acceptable. Thorough knowledge of laboratory safety =
principles is imperative. Considerable knowledge of industrial hygiene =
equipment, uses and methodology as well as the ability to calibrate and =
maintain monitoring equipment is necessary.=20
Knowledge of health and safety and environmental regulations is also =
necessary. Ability to prepare clear, accurate and informative reports =
including investigative findings, conclusions, and recommendations along =
with superior interpersonal and oral presentation skills are needed.
UMDNJ offers a competitive salary and a comprehensive benefits package. =
Please send your resume to: Ms. Sandi Speich, Department of Human =
Resources, UMDNJ, Primary Care Center, Suite 111, 40 E. Laurel Road, =
Stratford, NJ 08084. UMDNJ is an Affirmative Action/Equal Opportunity =
Employer, m/f/d/v, and a member of the University Health System of New =
Jersey. We can only respond to candidates under consideration. For more =
information,=20
visit our website at: .
Janice Flesher, MS, CBSP
Principle Industrial Hygienist/Biosafety Officer
EOHSS - University Medical Dental School of NJ
97 Paterson St. #227
New Brunswick, NJ, 08901
(732) 235-8497 phone
(732) 235-8499 fax
fleshejk@umdnj.edu
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UMDNJ has a job opening near =
Philadelphia. =20
Biosafety people should respond to Human Resources as indicated, but if =
you have=20
questions, you could email Lindsey Kayman at kayman@umdnj.edu.
LABORATORY SAFETY SPECIALIST
UMDNJ, the University of Medicine & Dentistry of New Jersey, the =
nation's=20
largest comprehensive Health Sciences University, is seeking a =
Laboratory Safety=20
Specialist for the Stratford Campus, 25 minutes from downtown =
Philadelphia.
The salary range is from $43,269.66 - $60,172.99. Reporting to the =
Campus=20
Safety Manager, this individual will identify, evaluate, eliminate =
and/or=20
minimize occupational and environmental hazards in laboratories and =
related=20
premises.
In addition to a Master's degree in industrial hygiene, environmental =
or=20
occupational health sciences, chemistry or a related technical =
discipline, a=20
minimum of three (3) years of professional work experience in the field =
of=20
industrial hygiene, laboratory safety, or occupational/environmental =
health=20
prevention programs is required. A Bachelor's degree and five years of=20
experience in laboratory safety, industrial hygiene, biosafety or=20
occupational/environmental safety and health is also acceptable. =
Thorough=20
knowledge of laboratory safety principles is imperative. Considerable =
knowledge=20
of industrial hygiene equipment, uses and methodology as well as the =
ability to=20
calibrate and maintain monitoring equipment is necessary.
Knowledge of health and safety and environmental regulations is also=20
necessary. Ability to prepare clear, accurate and informative reports =
including=20
investigative findings, conclusions, and recommendations along with =
superior=20
interpersonal and oral presentation skills are needed.
UMDNJ offers a competitive salary and a comprehensive benefits =
package.=20
Please send your resume to: Ms. Sandi Speich, Department of Human =
Resources,=20
UMDNJ, Primary Care Center, Suite 111, 40 E. Laurel Road, Stratford, NJ =
08084.=20
UMDNJ is an Affirmative Action/Equal Opportunity Employer, m/f/d/v, and =
a member=20
of the University Health System of New Jersey. We can only respond to =
candidates=20
under consideration. For more information,
visit our website at: .
Janice Flesher, MS, CBSPPrinciple =
Industrial=20
Hygienist/Biosafety OfficerEOHSS - University Medical Dental School =
of=20
NJ97 Paterson St. #227New Brunswick, NJ, 08901(732) 235-8497 =
phone(732) 235-8499 faxfleshejk@umdnj.edu
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TITLE:Principle industrial Hygienist/Biosafety Officer
TEL;WORK;VOICE:732-235-8497
TEL;HOME;VOICE:609-620-1206
TEL;WORK;FAX:732-235-8499
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=========================================================================
Date: Fri, 5 Nov 1999 08:19:58 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: audit issues
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I know I previously asked this question, but due to a computer mishap I no
longer have the information.
For those of you at academic institutions:
Do you have a GMP facility on your campus?
Who is responsible for oversight of such a facility?
Who has been brought in as consultants in the design of and operation of
such a facility? e.g. writing and reviewing the SOPs for compliance with FDA
regs?
who are some consultants on FDA requirements for cGLP and cGMP?
you can email me directly
therese.stinnett@uchsc.edu
thanks
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
=========================================================================
Date: Fri, 5 Nov 1999 12:38:15 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Barbara Hontz
Organization: University of Scranton
Subject: Harris -80 Freezer
MIME-version: 1.0
Content-type: text/plain; charset=us-ascii
Content-transfer-encoding: 7bit
Hello everyone!
I apologize for the cross postings in advance. I was given a Harris -80
Freezer by a local company and I need a manual for it. Does anyone out
there have one of these that they may be able to fax me a copy of? Any
help would be appreciated!
Barbara Hontz
University of Scranton
Biology Stockroom Manager
800 Linden Street
Scranton, PA 18510
570-941-7558
hontzb3@uofs.edu
=========================================================================
Date: Fri, 5 Nov 1999 17:11:05 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Rachael Brooks
Subject: research notification
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hello everyone,
Our university is new to research but is really taking off. Our safety
committee (not just biosafety) is now wanting notification of any research
on campus. This came about because our Provost found out about one
faculty's Neisseria meningititis research in our campus paper. Our labs
have only level 2 organisms, no radiation or animal research. Before
implementing any policy I would like to know how other universities handle
the notification process and any other advice you may want to give. Do your
researchers submit all of their proposals to the committee, or only the
grants that have been approved? Thank you in advance for your help.
Rachael L. Brooks
Microbiology Lab Coordinator
Texas A&M University-Corpus Christi
6300 Ocean Drive, CS130
Corpus Christi, TX 78412
361-825-5870 office
361-825-2742 fax
=========================================================================
Date: Fri, 5 Nov 1999 14:26:33 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: research notification
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Hi, Rachael -
Please feel free to check our biosafety manual for details about our program
for research notification/registration/authorization. It's on our web site
at . Making such documents available on the
institutional web site is becoming a common practice and you can probably
find on-line biosafety manuals, forms and program details at the web sites
of many universities. I'll be happy to talk with you about specifics of our
program any time you'd like.
-- Glenn
------------------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
University of California, San Francisco
Voice 415-476-2097
Fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Rachael Brooks [mailto:rbrooks@FALCON.TAMUCC.EDU]
Sent: Friday, November 05, 1999 2:11 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: research notification
Hello everyone,
Our university is new to research but is really taking off. Our
safety
committee (not just biosafety) is now wanting notification of any research
on campus. This came about because our Provost found out about one
faculty's Neisseria meningititis research in our campus paper. Our labs
have only level 2 organisms, no radiation or animal research. Before
implementing any policy I would like to know how other universities handle
the notification process and any other advice you may want to give. Do your
researchers submit all of their proposals to the committee, or only the
grants that have been approved? Thank you in advance for your help.
Rachael L. Brooks
Microbiology Lab Coordinator
Texas A&M University-Corpus Christi
6300 Ocean Drive, CS130
Corpus Christi, TX 78412
361-825-5870 office
361-825-2742 fax
=========================================================================
Date: Sat, 6 Nov 1999 09:11:55 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: FYI
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
FYI
Ed Krisiunas, MT(ASCP), CIC, MPH
Director
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
Want to send this story to another AOL member? Click on the heart at the top
of this window.
Occupational Safety and Health Administration Stresses Employer Use of
NewMedical Advances; OSHA Revises Bloodborne Pathogens Compliance Directive
WASHINGTON, Nov. 5 /PRNewswire/ -- A new directive issued today by the
Occupational Safety and Health Administration will help minimize serious
health risks faced by workers exposed to blood and other potentially
infectious materials. Among the risks are human immunodeficiency virus (HIV),
hepatitis B and hepatitis C.
The directive guides OSHA's compliance officers in enforcing the standard
that covers occupational exposure to bloodborne pathogens and ensures
consistent inspection procedures are followed. It updates an earlier
directive issued in 1992 and reflects the availability of improved devices,
better treatment following exposure and OSHA policy interpretations.
"We must do everything we can to protect workers who may be at risk of
exposure to bloodborne diseases," said Secretary of Labor Alexis M. Herman.
"This directive doesn't place new requirements on employers, but it does
recognize and emphasize the advances made in medical technology. And it
reminds employers that they must use readily-available technology in their
safety and health programs."
The revised directive emphasizes the importance of an annual review of the
employer's bloodborne pathogens program and the use of safer medical devices
to help reduce needlesticks and other sharps injuries. OSHA does not advocate
the use of one particular medical device over another. The directive also
highlights basic work practices, personal protective equipment and
administrative controls.
The emphasis on engineering controls results from OSHA's request last year
for ideas and recommendations on ways to better protect workers from
contaminated needles or other sharp objects.
"We received nearly 400 comments from health care facilities, workers and
others," said OSHA Administrator Charles N. Jeffress. "They told us that safe
medical devices already available are effective in controlling hazards and
that wider use of such devices would reduce thousands of injuries each year."
The revised directive also includes detailed instructions to compliance
officers on inspections of multi-employer worksites, such as home health
services, employment agencies, personnel services, physicians and health care
professionals in independent practices and independent contractors.
Also included in the directive are decontamination requirements, guidelines
on hepatitis vaccinations and post exposure treatments, and employee
training.
OSHA issued a final regulation on occupational exposure to bloodborne
pathogens in 1991 to protect nearly six million workers in health care and
related occupations at risk of exposure to bloodborne diseases. Jeffress said
the agency will review the standard to determine whether its revision is
warranted.
The directive can be accessed from the OSHA home page at
under the "Directives" link. Copies can also be obtained from the agency's
publications office by calling 202-693-1888.
The text of this news release is on the Internet World Wide Web at
. Information on this news release will be made available
to sensory impaired individuals upon request. Voice phone: 202-693-1999.
Highlights of OSHA's Compliance Directive CPL 2-2.44D Enforcement Procedures
for the Occupational Exposure to Bloodborne Pathogens
OSHA first published the bloodborne pathogens standard in 1991 because of a
significant health risk associated with occupational exposure to blood and
other potentially infectious materials that may contain bloodborne pathogens
-- or microorganisms -- that cause bloodborne diseases. The compliance
directive detailing enforcement procedures for the standard was published on
March 6, 1992 (the effective date of the standard).
During the past seven years, significant medical advances have occurred that
help control bloodborne pathogens. In addition, OSHA has clarified the
standard through written interpretations. The emerging technology, coupled
with new information on the control of bloodborne pathogens, necessitated a
revision in the compliance directive. Following is a summary of some of the ke
y revisions:
Annual Review of Exposure Control Plan -- employers must ensure that their
plans reflect consideration and use of commercially available safer
medical devices.
Engineering Controls and Work Practices -- emphasizes the use of effective
engineering controls, to include safer medical devices, work practices,
administrative controls and personal protective equipment.
Emphasizes that employers should rely on relevant evidence in addition to
FDA approval to ensure effectiveness of devices designed to prevent
exposure to bloodborne pathogens.
Multi-Employer Worksites -- focuses on employment agencies, personnel
services, home health services, independent contractors, and physicians in
independent practice.
Adds most recent guidelines from the Centers for Disease Control on
vaccinations against the Hepatitis B virus. Incorporates CDC's guidelines
on post exposure evaluation and follow-up for HIV and the Hepatitis C
virus.
Requires effective training and education for employees whenever safer
devices are implemented. Stresses "interactive" training sessions rather
than just the use of films or videos that do not provide the opportunity
for discussion with a qualified trainer.
Replaces and updates appendices. Includes the following: examples of
committees in health care facilities; sample engineering control
evaluation forms; an Internet resource list; a "fill-in-the-blanks" sample
exposure control plan; and CDC guidelines pertaining to HIV exposure,
control and prevention of hepatitis C, and hepatitis B vaccinations.
SOURCE U.S. Department of Labor, Occupational Safety and Health
Administration
CO: U.S. Department of Labor, Occupational Safety and Health Administration
ST: District of Columbia
IN: HEA MTC
SU: EXE
11/05/1999 16:47 EST
=========================================================================
Date: Sat, 6 Nov 1999 22:06:48 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Diane Fleming
Subject: Fwd: Microbes on TV -- See it on PBS!
MIME-Version: 1.0
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I thought this program on microbes might be of interest to the ListServ
readers who have access to it. Diane
Diane O. Fleming, Ph.D., CBSP
Biosafety Consultant
15611 Plumwood Court
Bowie, MD 20716-1434
Tel 301-249-3951
FAX 301-249-8837
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Message-ID:
From: PublicCommunications_Alert
To: ASM Member
Subject: Microbes on TV -- See it on PBS!
Date: Fri, 5 Nov 1999 15:46:45 -0500
Mime-Version: 1.0
Errors-To: PublicCommunications_Alert@mail.
Originator: PublicCommunications_Alert@mail.
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ASM is proud to announce to its membership the premiere of the
documentary series, Intimate Strangers: Unseen Life on Earth,
a four-part series that examines the wonders of the microbial
world. The series premiers on November 9th at 8:00 p.m. (EST)
on PBS with the TREE OF LIFE episode. The series will continue
on subsequent Tuesdays through November 30.
Many individual ASM members have participated in the
development of Intimate Strangers: Unseen Life on Earth
and the Society is a major sponsor of the entire Microbial
Literacy Collaborative. It is with great enthusiasm that we
encourage all of you to tune in to your local PBS station to
watch Intimate Strangers: Unseen Life on Earth. Visit
for local listings.
Below this note we have included the ASM Press Release
concerning Intimate Strangers. Please feel free to forward
this release to your colleagues and students. This is a
wonderful opportunity to promote this new series
highlighting our science.
For more information on Intimate Strangers and the other
components of ASM's public communications campaign, the
Microbial Literacy Collaborative, visit
. Here you will find more
information on the series and links to ASM's extensive
microbial literacy efforts.
We hope you enjoy the show.
Julian Davies, Ph.D.
President
American Society for Microbiology
Jay Grimes, Ph.D.
Chair, Communications Committee
American Society for Microbiology
ASM Press Release -- November 5, 19998
MEDIA INFORMATION
Contact: Barbara Hyde
ASM Communications Director
202-942-9206
bhyde@
INTIMATE STRANGERS TV SERIES
OPENS WINDOWS ON MICROBIAL WORLD
WASHINGTON, DC-Nov. 1, 1999-They are known as bacteria,
viruses, fungi, and protozoa, or maybe just germs.
These normally invisible microbes represent more than
50% of all life on Earth. We share our planet with these
smallest of creatures that are often extraordinarily complex,
and depend on them for life itself. The wonders, mysteries,
and potential of the vast microbial world around us are
explored in INTIMATE STRANGERS: UNSEEN LIFE ON EARTH,
a four-part series scheduled to premiere on PBS beginning
Tuesday, November 9, 8-9 p.m., EST (Check local listings).
The series will continue on subsequent Tuesdays through
November 30.
Each of the four one-hour programs examines the microbial
world from a different perspective, but all make the same
fundamental point: our continued existence on Earth is going
to be determined in part by the well-being of the planet s
microbial population-and it is essential for us to increase
our understanding of this crucial component of
the web of life.
Program One, Tree of Life, investigates ancient microbe DNA
and its relation to all life on Earth.
Program Two, Keepers of the Biosphere, illustrates how
microbes drive the chemistry of life, affect global climate,
and do most of the recycling that keeps the world habitable.
The hour showcases scientists who are exploring our reliance
on this invisible world.
Program Three, Dangerous Friends and Friendly Enemies,
focuses on how infectious diseases occur when our relationship
with microbes changes or an intruder invades.
Program Four, Creators of the Future, introduces scientists
who are turning to microbes for solution to the challenges of
the 21st century, such as reclaiming our damaged environment
and feeding a growing world population.
The series, available in high-definition television format
(HDTV), takes viewers from the nuclear wasteland of Chernobyl
to the boiling volcanic vents where life possibly arose, from
the breadth of the oceans to the world teeming in a drop of water.
Stories of scientists and microbes are told through
state-of-the-art microphotography, photorealistic
3-D animation, and new footage shot inside some of the
most cutting-edge laboratories.
The Intimate Strangers TV series is the cornerstone of a
broad-based science education initiative, the Microbial
Literacy Collaborative, led by the American Society for
Microbiology. Efforts also included in the MLC include
*A telecourse, Unseen Life on Earth: An Introduction to
Microbiology based on the TV series and distributed by
the Annenberg/CPB Project, which funded its development;
* MicrobeWorld, a set of hands-on activities for
community and school use, distributed nationally by science
and technology museums and community organizations;
* MicrobeWorld Mentors, a series of youth leadership
summits to develop students who can mentor their peers in
exploring the microbial world;
* , an educational website that
provides an interactive entry point into the invisible
world of microbes; and
* Intimate Strangers, a richly illustrated companion
book to the TV series, to be published by ASM Press in
January 2000.Intimate Strangers: Unseen Life on Earth
is a production of Baker & Simon Associates in association
with Oregon Public Broadcasting. The American Society for
Microbiology provided scientific oversight for the series.
The series is presented on PBS by Oregon Public
Broadcasting, with major funding from the National Science
Foundation, the Department of Energy, the American Society
for Microbiology, and the Annenberg/CPB
Project. Additional funding is provided by the Arthur
Vining Davis Foundations, the Corporation for Public
Broadcasting, and the Foundation for Microbiology.
The American Society for Microbiology, headquartered in
Washington, DC, is the oldest and largest single life
science society, with 43,000 members worldwide. The mission of
the American Society for Microbiology is to promote
microbiological sciences and their application for the
common good. The Society accomplishes this by disseminating
information, stimulating research, promoting education,
advancing the profession and promoting the application
of the microbiological sciences.
ASM carefully reviews email for appropriateness and interest
to the member. If you do not want to receive these email
alerts in the future, please send a message to subscriptions@
requesting to unsubscribe. Be
sure to include your name and ASM member number.
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=========================================================================
Date: Mon, 8 Nov 1999 09:52:03 -0500
Reply-To: rubockpa@UMDNJ.EDU
Sender: A Biosafety Discussion List
From: Paul Rubock
Organization: eohss-umdnj
Subject: downloadable photo
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Does anyone have photos in downloadable form of centrifuge safety
covers; I need them for a biosafety manual.
Thank you,
Paul Rubock
=========================================================================
Date: Tue, 9 Nov 1999 16:05:55 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: Inoculation Loop Sterilization
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
In order to avoid the flaming technique to sterilize the inoculation loop
when using the biosafety cabinet, there are electric loop sterilizers.
Did anyone know the manufacturer and recommend model on the electric loop
sterilizers? I and even the local suppliers of biomedical devices are not
able to get this item.
Regards.
YK Wan
Safety Officer
Please reply when you receive the message. Thank you.
***** Yu Kwan WAN,
***** Safety Officer
***** The Chinese University of Hong Kong
***** Shatin, NT, Hong Kong
***** Email:
***** ulsoykwan@cuhk.edu.hk
***** ulsoykwan@
***** ulsoykwan@
***** ulsoykwan@
***** ulsoykwan@
=========================================================================
Date: Tue, 9 Nov 1999 08:58:37 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Inoculation Loop Sterilization
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
The one brand I know of is:
Bacti-Cinerator made by Oxford Labware, a division fo Sherwood Medical, St.
Louis, MO.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Tue, 9 Nov 1999 09:02:15 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Greg Merkle
Organization: Wright State University
Subject: Re: Inoculation Loop Sterilization
MIME-version: 1.0
Content-type: text/plain; charset=us-ascii
Content-transfer-encoding: 7bit
Check with Fisher Scientific in the US. The product on page
1092 of their 98/99 catalog is called a Bacti-Cinerator III
Sterilizer (Oxford No. 8889-01007, Catalog 14-489, and cost
of $235.00) Give them a call at 1-800-766-7000.
Greg Merkle
Wan Yu Kwan wrote:
>
> In order to avoid the flaming technique to sterilize the inoculation loop
> when using the biosafety cabinet, there are electric loop sterilizers.
>
> Did anyone know the manufacturer and recommend model on the electric loop
> sterilizers? I and even the local suppliers of biomedical devices are not
> able to get this item.
>
> Regards.
>
> YK Wan
> Safety Officer
> Please reply when you receive the message. Thank you.
>
> ***** Yu Kwan WAN,
> ***** Safety Officer
> ***** The Chinese University of Hong Kong
> ***** Shatin, NT, Hong Kong
>
> ***** Email:
> ***** ulsoykwan@cuhk.edu.hk
> ***** ulsoykwan@
> ***** ulsoykwan@
> ***** ulsoykwan@
> ***** ulsoykwan@
=========================================================================
Date: Tue, 9 Nov 1999 16:14:59 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Cheri Marcham
Subject: bsl3 utility checklist
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
We want to perform utility inspections at our BSL3 lab (plumbing, HVAC,
alarms, emergency backup) etc. and are meeting to develop a checklist of the
things we should be looking for and testing above and beyond the BSC
certification issue. Have any of you developed such a checklist we can use
to get started?
Cheri Marcham
Environmental Health and Safety Office
The University of Oklahoma Health Sciences Center
cheri-marcham@ouhsc.edu
=========================================================================
Date: Tue, 9 Nov 1999 20:20:21 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Esmeralda Party
Subject: Re: bsl3 utility checklist
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Cheri,
If you send me a fax number I can send you some of our checklists.
Esmeralda
At 04:14 PM 11/9/99 -0600, you wrote:
>We want to perform utility inspections at our BSL3 lab (plumbing, HVAC,
>alarms, emergency backup) etc. and are meeting to develop a checklist of the
>things we should be looking for and testing above and beyond the BSC
>certification issue. Have any of you developed such a checklist we can use
>to get started?
>
>
>Cheri Marcham
>Environmental Health and Safety Office
>The University of Oklahoma Health Sciences Center
>cheri-marcham@ouhsc.edu
>
>
=========================================================================
Date: Wed, 10 Nov 1999 08:16:49 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: bsl3 utility checklist
MIME-Version: 1.0
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boundary="----=_NextPart_000_0788_01BF2B53.EFDA3C00"
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Esmeralda,
Could you send those lists to me also?
Janice Flesher, MS, CBSP
Principle Industrial Hygienist/Biosafety Officer
EOHSS - University Medical Dental School of NJ
97 Paterson St. #227
New Brunswick, NJ, 08901
(732) 235-8497 phone
(732) 235-8499 fax
fleshejk@umdnj.edu
-----Original Message-----
From: Esmeralda Party
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Tuesday, November 09, 1999 8:23 PM
Subject: Re: bsl3 utility checklist
>Cheri,
>
>If you send me a fax number I can send you some of our checklists.
>
>Esmeralda
>
>
>
>At 04:14 PM 11/9/99 -0600, you wrote:
>>We want to perform utility inspections at our BSL3 lab (plumbing, HVAC,
>>alarms, emergency backup) etc. and are meeting to develop a checklist of
the
>>things we should be looking for and testing above and beyond the BSC
>>certification issue. Have any of you developed such a checklist we can use
>>to get started?
>>
>>
>>Cheri Marcham
>>Environmental Health and Safety Office
>>The University of Oklahoma Health Sciences Center
>>cheri-marcham@ouhsc.edu
>>
>>
>
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FN:Janice K Flesher, MS
ORG:University Medical Dental of NJ;EOHSS
TITLE:Principle industrial Hygienist/Biosafety Officer
TEL;WORK;VOICE:732-235-8497
TEL;HOME;VOICE:609-620-1206
TEL;WORK;FAX:732-235-8499
ADR;WORK:;;97 Paterson Street Ste 227;New Brunswick;NJ;08903;USA
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227=3D0D=3D0ANew Brunswick, NJ 08903=3D0D=3D0AUSA
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=========================================================================
Date: Wed, 10 Nov 1999 12:41:05 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: David Mount
Subject: Continuing search for BSO
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
PLEASE NOTE: The following announcement for a Biosafety Officer at the
University of Arizona was placed on this listserv on Sept. 21. Because of
the small response, our search committee has requested that we resubmit the
announcement. An abbreviated outline is given below. More information may
be found at .
Position Summary:
The University Biosafety Officer (BSO) functions as an agent of the
Institutional Biosafety Committee (IBC) in the routine administration of
the biological safety program at the University of Arizona.
Qualifications:
This job requires an advanced degree in microbiology, biochemistry,
genetics, molecular biology, or a closely related field. A Ph.D. is
desirable. The Biosafety Officer should have a working knowledge of NIH
Recombinant DNA guidelines, the Center for Disease Control regulations for
microbial pathogens, and relevant USDA, EPA and OSHA regulations.
Experience in biological risk assessment and public health is desirable.
The position requires excellent written and oral communication skills and
computer expertise. Registration as a Certified Biological Safety
Professional through the
American Biological Association is highly desirable.
To apply, please submit a cover letter, resume and the names and contact
information for two references to:
Institutional Biosafety Committee
Life Sciences South, Room 333
PO BOX 210106
University of Arizona
Tucson, AZ 85721
Review of materials will continue until the position is filled.
The University of Arizona is an EEO/AA Employer.
David W. Mount, Professor
Molecular and Cellular Biology
University of Arizona
Tucson, AZ 85721
(520) 621 3151 office, 621 4814 (lab),
621 6159 FAX, 577 3140 home
mount@u.arizona.edu
=========================================================================
Date: Wed, 10 Nov 1999 14:50:15 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Gerry.Griffin"
Subject: ethanol & flame in bsc = fire
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; CHARSET=US-ASCII
We had an incident here recently where a new lab tech was removing lymph
nodes from a mouse for tissue culturing. Surgical tools (forceps,
scissors) were dipped in a beaker with a small amt , @ 20 ml of 70%
alcohol and then passed through a flame). Apparently, a lightweight
plastic beaker was being used. It was knocked over, alcohol spilled on
the lab tech's coat and he ended up with 2nd degree burns on his hands.
I immediately recommended that the container should be stabilized to
prevent knocking it over. The PI doesn't think there's anyway to get
around using the alcohol and the flame. It's not practical to use a
new tool each time. Alcohol or water on the instruments may lyse the
cells. I'm checking in to see what others here think.
----------------------------------------
Gerry Griffin
Environmental Services
Email: Gerry.Griffin@med.nyu.edu
=========================================================================
Date: Wed, 10 Nov 1999 15:11:17 +0500
Reply-To: speaker@ehs.psu.edu
Sender: A Biosafety Discussion List
From: Curt Speaker
Organization: UNIVERSITY SAFETY
Subject: Re: ethanol & flame in bsc = fire
In-Reply-To:
Gerry:
My thought would be "why use the alcohol?" It seems very
unlikely that any organisms could withstand the heat of being
flamed. Flaming alone should be able to accomplish the job.
Reminds me of an incident at a previous employer where someone
sprayed down the inside of a BSC with 70% ethanol BEFORE they
extinguished the spirit lamp that was inside. Created a huge blue
flash of flame and burned the HEPA filter. Expensive mistake, to
say the least...
Please engage brain before putting hands in motion...
Curt
Curt Speaker
Biosafety Officer
Penn State University
Environmental Health and Safety
speaker@ehs.psu.edu
^...^
(O_O)
=(Y)=
"""
=========================================================================
Date: Wed, 10 Nov 1999 15:19:21 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Barbara Hontz
Organization: University of Scranton
Subject: Re: Harris -80 freezer
MIME-version: 1.0
Content-type: text/plain; charset=us-ascii
Content-transfer-encoding: 7bit
Just a note of thanks to everyone who responded to my plea. I found the
Harris website very helpful and got the information I needed. My
original search of the web didn't produce the correct company name.
Thank you!
Barb Hontz
University of Scranton
Biology Department
=========================================================================
Date: Wed, 10 Nov 1999 17:16:47 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Daniel King
Subject: Re: ethanol & flame in bsc = fire
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
We use a dry sterilizer as a safe fast alternative to alcohol flaming.
Instruments are placed in a well filled with glass beads that are heated
to 250 C.
The unit we use came from Inotech Biosystems International, Lansing,
Michigan, 1-800-635-4070
Daniel J. (Jack) King
USDA, ARS, Southeast Poultry Research Laboratory
934 College Station Road
Athens, GA 30605
706-546-3407 Phone
706-546-3161 FAX
dking@asrr.
jking@seprl.
On Wed, 10 Nov 1999, Gerry.Griffin wrote:
> We had an incident here recently where a new lab tech was removing lymph
> nodes from a mouse for tissue culturing. Surgical tools (forceps,
> scissors) were dipped in a beaker with a small amt , @ 20 ml of 70%
> alcohol and then passed through a flame). Apparently, a lightweight
> plastic beaker was being used. It was knocked over, alcohol spilled on
> the lab tech's coat and he ended up with 2nd degree burns on his hands.
> I immediately recommended that the container should be stabilized to
> prevent knocking it over. The PI doesn't think there's anyway to get
> around using the alcohol and the flame. It's not practical to use a
> new tool each time. Alcohol or water on the instruments may lyse the
> cells. I'm checking in to see what others here think.
>
> ----------------------------------------
> Gerry Griffin
> Environmental Services
> Email: Gerry.Griffin@med.nyu.edu
>
=========================================================================
Date: Wed, 10 Nov 1999 14:14:32 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Dan Shawler
Subject: Re: ethanol & flame in bsc = fire
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I had a similar incident happen to me early in my career. Fortunately I
only ended up setting fire to the (already dead) mice and not to myself. A
few weeks after that, I set fire to the inside of the BSC when my bunsen
burner burned a hole through the plastic tubing that fed the gas to the
burner. After those experiences, I stopped using flames of any kind in the
BSC. I generally have fewer contamination problems than my coworkers (I
still work part time in the lab). As for the instruments for removing mouse
and rat organs, I still dip them in ethanol, but now I just let them drip
dry for a few seconds. The draft in the hood appears to do a good job of
drying them and I don't have any problems with cell viability.
Dan Shawler
Biological Safety Officer
Sidney Kimmel Cancer Center
-----Original Message-----
From: Gerry.Griffin [mailto:Gerry.Griffin@MED.NYU.EDU]
Sent: Wednesday, November 10, 1999 11:50 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: ethanol & flame in bsc = fire
We had an incident here recently where a new lab tech was removing lymph
nodes from a mouse for tissue culturing. Surgical tools (forceps,
scissors) were dipped in a beaker with a small amt , @ 20 ml of 70%
alcohol and then passed through a flame). Apparently, a lightweight
plastic beaker was being used. It was knocked over, alcohol spilled on
the lab tech's coat and he ended up with 2nd degree burns on his hands.
I immediately recommended that the container should be stabilized to
prevent knocking it over. The PI doesn't think there's anyway to get
around using the alcohol and the flame. It's not practical to use a
new tool each time. Alcohol or water on the instruments may lyse the
cells. I'm checking in to see what others here think.
----------------------------------------
Gerry Griffin
Environmental Services
Email: Gerry.Griffin@med.nyu.edu
=========================================================================
Date: Wed, 10 Nov 1999 17:15:23 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Greg Merkle
Organization: Wright State University
Subject: Recommeded Handling Procedures for HIV Cultures
MIME-version: 1.0
Content-type: text/plain; charset=us-ascii
Content-transfer-encoding: 7bit
I question has come up about the handling of growing
cultures of HIV. HIV is considered to be a BSL2 virus and
is handled accordingly with the addition of containment
practices for a BSL3 facility. Should the handling be
considered to be BSL2 or BSL3? The question about this came
up during a discussion of handling procedures for the HIV
and whether or not there was a potential problem with the
methods currently being used to handle liquid waste from the
cultures. Presently liquid is being treated with a
non-chlorine disinfectant, I do have the name of the
product, and autoclaved before going to a sanitary drain.
Aside from the 4th ed. of the BMBL what other good
references are available to extract information from? Who
at the CDC would be a good contact person to point me in the
right direction to assist the researcher in question make
the right decision in any changes in handling that might be
needed.
Thank you for your help, it is greatly appreciated.
Greg Merkle
Senior Industrial Hygienist
=========================================================================
Date: Thu, 11 Nov 1999 09:23:59 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: Re: ethanol & flame in bsc = fire
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Gerry
Because of the use of the light-weight plastic beaker, it can be tip over
easily by the moment of the heavy tools. I recommend a stand of wide-base
area preferably fire-resistant and chemical resistant would be used.
At 02:50 PM 11/10/99 -0500, you wrote:
>We had an incident here recently where a new lab tech was removing lymph
>nodes from a mouse for tissue culturing. Surgical tools (forceps,
>scissors) were dipped in a beaker with a small amt , @ 20 ml of 70%
>alcohol and then passed through a flame). Apparently, a lightweight
>plastic beaker was being used. It was knocked over, alcohol spilled on
>the lab tech's coat and he ended up with 2nd degree burns on his hands.
>I immediately recommended that the container should be stabilized to
>prevent knocking it over. The PI doesn't think there's anyway to get
>around using the alcohol and the flame. It's not practical to use a
>new tool each time. Alcohol or water on the instruments may lyse the
>cells. I'm checking in to see what others here think.
>
>----------------------------------------
>Gerry Griffin
>Environmental Services
>Email: Gerry.Griffin@med.nyu.edu
>
>
Please reply when you receive the message. Thank you.
***** Yu Kwan WAN,
***** Safety Officer
***** The Chinese University of Hong Kong
***** Shatin, NT, Hong Kong
***** Email:
***** ulsoykwan@cuhk.edu.hk
***** ulsoykwan@
***** ulsoykwan@
***** ulsoykwan@
***** ulsoykwan@
=========================================================================
Date: Thu, 11 Nov 1999 09:10:42 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: BBP Safety Poster
MIME-Version: 1.0
Content-Type: text/plain
I was looking into developing a BBP Safety Poster. This poster would depict
safety precautions to take while working with potential BBP (transmission
routes, PPE, reporting an incident etc.).
Has anyone already completed this task? If so, would I be able to see
yours?
Thanks!
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Thu, 11 Nov 1999 11:58:02 -0500
Reply-To: rubockpa@UMDNJ.EDU
Sender: A Biosafety Discussion List
From: Paul Rubock
Organization: eohss-umdnj
Subject: Re: BBP Safety Poster
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Check out the catalogue from Lab Safety Supply; I know that they have something
that is useful and well-produced.
Schlank Bliss BM wrote:
> I was looking into developing a BBP Safety Poster. This poster would depict
> safety precautions to take while working with potential BBP (transmission
> routes, PPE, reporting an incident etc.).
>
> Has anyone already completed this task? If so, would I be able to see
> yours?
>
> Thanks!
> Bliss M. Schlank
> Biosafety Specialist
> AstraZeneca
> 1800 Concord Pike
> Wilmington DE 19850-5437
> 302.886.2185 Fax: 302.886.2909
> bliss.schlank@phwilm.
>
=========================================================================
Date: Thu, 11 Nov 1999 13:02:41 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stan Harts
Organization: University of North Carolina at Wilmington
Subject: Lab Animals and HVAC Systems
In-Reply-To:
MIME-version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
What standards, prudent practices, codes, rules of thumb or just
plain common sense do you guys and gals use to determine
whether HVAC systems that serve areas where lab animals are
housed/studies are the same systems (ie return air) as the
remainder of the building, including classrooms/offices.
It seems logical that areas where bedding, feeding etc. are kept
should be separate systems but how about small research
cubicles with skinner boxes etc where the animals only reside for a
few hours.
Sorry for duplicate postings, this is being submitted to BIOSAFTY
and SAFETY. Any help or guidance would be appreciated.
Stan H. Harts
Environmental Safety Manager
University of North Carolina at Wilmington
601 South College Road
Wilmington, NC 28403-3297
E-Mail: Hartss@uncwil.edu
Phone: (910) 962-7017
Fax: (910) 962-4014
=========================================================================
Date: Thu, 11 Nov 1999 11:08:16 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Al Jin
Subject: Re: Lab Animals and HVAC Systems
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Stan,
I'm sure you already know this but animals have more rights than humans.
I'm sure they would be very upset to find out their ventilation system is
being shared by humans. Joking aside, this is true. Animal Care regulations
are defined by (1) USDA in 9 CFR Chapter 1 Subchapter A, Animal Welfare.
Parts 1, 2. 3 and the Animal Welfare Ace of 1966 (PL89544), (2) USHHS-PHS
in PHS Policy on Humane Care and Use of Lab Animals, (3) DOT in 49 CFR
143(a)1: Hazards Material Regulations. Another useful document is the
National Research Council's Guide for the care and use of Laboratory
Animals.
On the other side of the coin, the human counter-parts would also be just
as annoyed to find out that the house ventilation system with return air is
being used for the care of animals. The OSHA regs under 29 CFR 1910.100
references ACGIH and ANSI Standards.The AGCIH Industrial Ventilation Book,
ANSI/ASHRAE 55 AND 55a-1995:Thermal Environmental Conditions for Human
Occupancy, ASHRAE 62-1989: Ventilation for Acceptable Indoor Air Quality,
and the proposed ASHRAE Standard 62-1989R: Ventilation for Acceptable
Indoor Air Quality are useful guidance documents.Other references sources
include the Uniform building code, National Fire Protection Association,
State Regs, Air Movement and Control Association (AMCA), and NIH .
I hope this helps.
Alfred Jin, BSO, IH, MS, CBSP, M(ASCP), BSM(ASM), CM(ACM),
Hazards Control Department,
Lawrence Livermore National Laboratory,
7000 East Avenue MS-289, Livermore, CA 94550,
Phone:925 423-7385, Fax:423-1086,
Jin2@
>What standards, prudent practices, codes, rules of thumb or just
>plain common sense do you guys and gals use to determine
>whether HVAC systems that serve areas where lab animals are
>housed/studies are the same systems (ie return air) as the
>remainder of the building, including classrooms/offices.
>
>It seems logical that areas where bedding, feeding etc. are kept
>should be separate systems but how about small research
>cubicles with skinner boxes etc where the animals only reside for a
>few hours.
>
>Sorry for duplicate postings, this is being submitted to BIOSAFTY
>and SAFETY. Any help or guidance would be appreciated.
>Stan H. Harts
>Environmental Safety Manager
>University of North Carolina at Wilmington
>601 South College Road
>Wilmington, NC 28403-3297
>E-Mail: Hartss@uncwil.edu
>Phone: (910) 962-7017
>Fax: (910) 962-4014
=========================================================================
Date: Thu, 11 Nov 1999 10:56:48 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Dan Shawler
Subject: Re: Lab Animals and HVAC Systems
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
You have to check with the building engineers. If they don't know, there
should be a set of blueprints and specs that they can refer to.
-----Original Message-----
From: Stan Harts [mailto:hartss@UNCWIL.EDU]
Sent: Thursday, November 11, 1999 10:03 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Lab Animals and HVAC Systems
What standards, prudent practices, codes, rules of thumb or just
plain common sense do you guys and gals use to determine
whether HVAC systems that serve areas where lab animals are
housed/studies are the same systems (ie return air) as the
remainder of the building, including classrooms/offices.
It seems logical that areas where bedding, feeding etc. are kept
should be separate systems but how about small research
cubicles with skinner boxes etc where the animals only reside for a
few hours.
Sorry for duplicate postings, this is being submitted to BIOSAFTY
and SAFETY. Any help or guidance would be appreciated.
Stan H. Harts
Environmental Safety Manager
University of North Carolina at Wilmington
601 South College Road
Wilmington, NC 28403-3297
E-Mail: Hartss@uncwil.edu
Phone: (910) 962-7017
Fax: (910) 962-4014
=========================================================================
Date: Thu, 11 Nov 1999 16:56:47 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ninni Jacob
Subject: Monkey waste
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Do any of you have policies on handling excreta from macaque monkeys,
because of the potential risk from B-virus?
Do you treat the excreta in any way?
How do you dispose of it?
Thanks again for your help.
Ninni Jacob, CHP
Radiation and Biological Safety Officer
Office of Risk Management
Brown University - Box 1914
164 Angell Street
Providence, RI 02912
Tel:401 863 1738
Fax:401 863 7676
email: Ninni_Jacob@brown.edu
=========================================================================
Date: Fri, 12 Nov 1999 08:47:09 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Petty, Carol"
Subject: CDC Laboratory Registration (Select Agent Transfer)
MIME-Version: 1.0
Content-Type: text/plain
Hi!
Has anyone had to register with the CDC for select agent transfer tracking
system. We are working with some agents on the list and I requested the
application kit but did not get the associated forms with it. Does anyone
have them? Or are they available somewhere on the intranet that I am not
aware of. Let me know as soon as you can...time pressure. Thanks.
Carol L. Petty, C.I.H.
Industrial Hygienist
Phone: (505) 845-1076
Fax: (505) 845-1174
email: cpetty@
=========================================================================
Date: Fri, 12 Nov 1999 11:21:12 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ninni Jacob
Subject: Re: CDC Laboratory Registration (Select Agent Transfer)
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Carol:
You have to request the application at the following website:
There are certain exceptions, which you can look up at:
The exceptions are based on the LD50 and whether the agent is used for
biomedical research or not.
We have gone through this process, so give me a call if I can be of further
assistance.
Ninni Jacob, CHP
Radiation and Biological Safety Officer
Office of Risk Management
Brown University - Box 1914
164 Angell Street
Providence, RI 02912
Tel:401 863 1738
Fax:401 863 7676
email: Ninni_Jacob@brown.edu
=========================================================================
Date: Fri, 12 Nov 1999 08:31:24 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Clifford W. Bond"
Subject: Re: CDC Laboratory Registration (Select Agent Transfer)
In-Reply-To:
MIME-Version: 1.0
Content-Type: multipart/mixed;
boundary="----=_NextPart_000_0048_01BF2CE8.4E199F00"
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charset="iso-8859-1"
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Carol,
We scanned a bunch of the documents into Word to make it easier to fill out
the forms. The forms are attached. If you need others let me know. A
contact who may be able to help you is David Bressler at CDC (404 639-4418).
He handled our registration.
Cliff Bond
Clifford W. Bond, Professor
Department of Microbiology
Montana State University
Bozeman, MT 59717-3520
Email: umbcb@gemini.oscs.montana.edu
Internet:
Telephone: 406 994-4130
TeleFAX: 406 994-4926
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Petty, Carol
Sent: Friday, November 12, 1999 8:47 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: CDC Laboratory Registration (Select Agent Transfer)
Importance: High
Hi!
Has anyone had to register with the CDC for select agent transfer tracking
system. We are working with some agents on the list and I requested the
application kit but did not get the associated forms with it. Does anyone
have them? Or are they available somewhere on the intranet that I am not
aware of. Let me know as soon as you can...time pressure. Thanks.
Carol L. Petty, C.I.H.
Industrial Hygienist
Phone: (505) 845-1076
Fax: (505) 845-1174
email: cpetty@
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\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx2973\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl
\brdrs\brdrw10 \clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx6054\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx9135
\row }\pard \ql \li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 =
{\f1\cgrid0 ( ) Lassa fever virus}{\b\f1\fs22\cgrid0 \cell }{\f1\cgrid0 =
( ) }{\i\f1\cgrid0 Clostridium botulinum}{\b\f1\fs22\cgrid0 \cell =
}{\f1\cgrid0 ( ) Conotoxins}{\b\f1\fs22\cgrid0=20
\cell }\pard \ql \li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 =
{\b\f1\fs22\cgrid0 \trowd \trgaph108\trleft-108\trbrdrt\brdrs\brdrw10 =
\trbrdrl\brdrs\brdrw10 \trbrdrb\brdrs\brdrw10 \trbrdrr\brdrs\brdrw10 =
\trbrdrh\brdrs\brdrw10 \trbrdrv
\brdrs\brdrw10 \trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3 =
\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx2973\clvertalt\clbrdrt\brdrs\brdrw10=20
\clbrdrl\brdrs\brdrw10 \clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx6054\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081=20
\cellx9135\row }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\f1\cgrid0 ( ) =
Marburg virus}{\b\f1\fs22\cgrid0 \cell }{\f1\cgrid0 ( ) }{\i\f1\cgrid0 =
Francisella tularensis}{\b\f1\fs22\cgrid0 \cell }{\f1\cgrid0 ( ) =
Diacetoxyscirpenol}{
\b\f1\fs22\cgrid0 \cell }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\b\f1\fs22\cgrid0 =
\trowd \trgaph108\trleft-108\trbrdrt\brdrs\brdrw10 =
\trbrdrl\brdrs\brdrw10 \trbrdrb\brdrs\brdrw10 \trbrdrr\brdrs\brdrw10 =
\trbrdrh\brdrs\brdrw10=20
\trbrdrv\brdrs\brdrw10 =
\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3 =
\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx2973\clvertalt\clbrdrt
\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 \clbrdrb\brdrs\brdrw10 =
\clbrdrr\brdrs\brdrw10 \cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx6054\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10=20
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx9135\row }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\f1\cgrid0 ( ) =
Rift Valley fever virus\tab }{\b\f1\fs22\cgrid0 \cell }{\f1\cgrid0 ( ) =
}{\i\f1\cgrid0 Yersinia pestis}{\b\f1\fs22\cgrid0=20
\cell }{\f1\cgrid0 (}{\b\f1\cgrid0 xx}{\f1\cgrid0 ) =
Ricin}{\b\f1\fs22\cgrid0 \cell }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\b\f1\fs22\cgrid0 =
\trowd \trgaph108\trleft-108\trbrdrt\brdrs\brdrw10 =
\trbrdrl\brdrs\brdrw10 \trbrdrb
\brdrs\brdrw10 \trbrdrr\brdrs\brdrw10 \trbrdrh\brdrs\brdrw10 =
\trbrdrv\brdrs\brdrw10 =
\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3 =
\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10=20
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx2973\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx6054\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10=20
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx9135\row }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\f1\cgrid0 ( ) =
South American Haemorrhagic fever viruses}{\b\f1\fs22\cgrid0 \cell \cell =
}{
\f1\cgrid0 ( ) Saxitoxin}{\b\f1\fs22\cgrid0 \cell }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\b\f1\fs22\cgrid0 =
\trowd \trgaph108\trleft-108\trbrdrt\brdrs\brdrw10 =
\trbrdrl\brdrs\brdrw10 \trbrdrb\brdrs\brdrw10 \trbrdrr\brdrs\brdrw10=20
\trbrdrh\brdrs\brdrw10 \trbrdrv\brdrs\brdrw10 =
\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3 =
\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx2973
\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx6054\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10=20
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx9135\row }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\f1\cgrid0 ( =
) Junin}{\b\f1\fs22\cgrid0 \cell Rickettsiae\cell }{\f1\cgrid0 =
(}{\b\f1\cgrid0 xx}{\f1\cgrid0 ) Shigatoxin}{
\b\f1\fs22\cgrid0 \cell }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\b\f1\fs22\cgrid0 =
\trowd \trgaph108\trleft-108\trbrdrt\brdrs\brdrw10 =
\trbrdrl\brdrs\brdrw10 \trbrdrb\brdrs\brdrw10 \trbrdrr\brdrs\brdrw10 =
\trbrdrh\brdrs\brdrw10=20
\trbrdrv\brdrs\brdrw10 =
\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3 =
\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx2973\clvertalt\clbrdrt
\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 \clbrdrb\brdrs\brdrw10 =
\clbrdrr\brdrs\brdrw10 \cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx6054\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10=20
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx9135\row }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\f1\cgrid0 ....( =
) Machupo}{\b\f1\fs22\cgrid0 \cell }{\f1\cgrid0 ( ) }{\i\f1\cgrid0 =
Coxiella bumetii}{\b\f1\fs22\cgrid0 \cell }{\f1\cgrid0=20
( ) Staphylococcal enterotoxins}{\b\f1\fs22\cgrid0 \cell }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\b\f1\fs22\cgrid0 =
\trowd \trgaph108\trleft-108\trbrdrt\brdrs\brdrw10 =
\trbrdrl\brdrs\brdrw10 \trbrdrb\brdrs\brdrw10 \trbrdrr
\brdrs\brdrw10 \trbrdrh\brdrs\brdrw10 \trbrdrv\brdrs\brdrw10 =
\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3 =
\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081=20
\cellx2973\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx6054\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr
\brdrs\brdrw10 \cltxlrtb\clftsWidth3\clwWidth3081 \cellx9135\row }\pard =
\ql \li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\f1\cgrid0 =
....( ) Sabia}{\f1\fs22\cgrid0 \cell }{\f1\cgrid0 ( ) }{\i\f1\cgrid0 =
Rickettsia prowazekii}{\b\f1\fs22\cgrid0=20
\cell }{\f1\cgrid0 ( ) Tetrodotoxin}{\b\f1\fs22\cgrid0 \cell }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\b\f1\fs22\cgrid0 =
\trowd \trgaph108\trleft-108\trbrdrt\brdrs\brdrw10 =
\trbrdrl\brdrs\brdrw10 \trbrdrb\brdrs\brdrw10 \trbrdrr
\brdrs\brdrw10 \trbrdrh\brdrs\brdrw10 \trbrdrv\brdrs\brdrw10 =
\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3 =
\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081=20
\cellx2973\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx6054\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr
\brdrs\brdrw10 \cltxlrtb\clftsWidth3\clwWidth3081 \cellx9135\row }\pard =
\ql \li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\f1\cgrid0 =
( ) Flexal}{\b\f1\fs22\cgrid0 \cell }{\f1\cgrid0 ( ) }{\i\f1\cgrid0 =
Rickettsia nckettsii}{\b\f1\fs22\cgrid0=20
\cell }{\f1\cgrid0 ( ) T-2 toxin}{\b\f1\fs22\cgrid0 \cell }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\b\f1\fs22\cgrid0 =
\trowd \trgaph108\trleft-108\trbrdrt\brdrs\brdrw10 =
\trbrdrl\brdrs\brdrw10 \trbrdrb\brdrs\brdrw10 \trbrdrr
\brdrs\brdrw10 \trbrdrh\brdrs\brdrw10 \trbrdrv\brdrs\brdrw10 =
\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3 =
\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081=20
\cellx2973\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx6054\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr
\brdrs\brdrw10 \cltxlrtb\clftsWidth3\clwWidth3081 \cellx9135\row }\pard =
\ql \li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\f1\cgrid0 =
( ) Guanarito}{\b\f1\fs22\cgrid0 \cell \cell \cell }\pard=20
\ql \li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 =
{\b\f1\fs22\cgrid0 \trowd \trgaph108\trleft-108\trbrdrt\brdrs\brdrw10 =
\trbrdrl\brdrs\brdrw10 \trbrdrb\brdrs\brdrw10 \trbrdrr\brdrs\brdrw10 =
\trbrdrh\brdrs\brdrw10 \trbrdrv\brdrs\brdrw10=20
\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3 =
\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx2973\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl
\brdrs\brdrw10 \clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx6054\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx9135
\row }\pard \ql \li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 =
{\f1\cgrid0 ( )Tick-borne encephalitis complex =
viruses}{\b\f1\fs22\cgrid0 \cell Fungi\cell \cell }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\b\f1\fs22\cgrid0 =
\trowd \trgaph108\trleft-108\trbrdrt\brdrs\brdrw10 =
\trbrdrl\brdrs\brdrw10 \trbrdrb\brdrs\brdrw10 \trbrdrr\brdrs\brdrw10 =
\trbrdrh\brdrs\brdrw10 \trbrdrv\brdrs\brdrw10 =
\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3 =
\clvertalt\clbrdrt\brdrs\brdrw10=20
\clbrdrl\brdrs\brdrw10 \clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx2973\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081=20
\cellx6054\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx9135\row }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\f1\cgrid0=20
( ) Variola major virus (Smallpox)}{\b\f1\fs22\cgrid0 \cell }{\f1\cgrid0 =
( ) }{\i\f1\cgrid0 Coccidioides immitis}{\b\f1\fs22\cgrid0 \cell \cell =
}\pard \ql \li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 =
{\b\f1\fs22\cgrid0 \trowd=20
\trgaph108\trleft-108\trbrdrt\brdrs\brdrw10 \trbrdrl\brdrs\brdrw10 =
\trbrdrb\brdrs\brdrw10 \trbrdrr\brdrs\brdrw10 \trbrdrh\brdrs\brdrw10 =
\trbrdrv\brdrs\brdrw10 =
\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3 =
\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl
\brdrs\brdrw10 \clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx2973\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx6054
\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx9135\row }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\f1\cgrid0=20
( ) Venezuelan Equine Encephalitis virus}{\b\f1\fs22\cgrid0 \cell \cell =
\cell }\pard \ql \li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 =
{\b\f1\fs22\cgrid0 \trowd \trgaph108\trleft-108\trbrdrt\brdrs\brdrw10 =
\trbrdrl\brdrs\brdrw10 \trbrdrb
\brdrs\brdrw10 \trbrdrr\brdrs\brdrw10 \trbrdrh\brdrs\brdrw10 =
\trbrdrv\brdrs\brdrw10 =
\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3 =
\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10=20
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx2973\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx6054\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10=20
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx9135\row }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\f1\cgrid0 =
(}{\b\f1\cgrid0 xx}{\f1\cgrid0 ) Viruses causing hantavirus pulmonary =
syndrome}{
\b\f1\fs22\cgrid0 \cell \cell \cell }\pard \ql =
\li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 {\b\f1\fs22\cgrid0 =
\trowd \trgaph108\trleft-108\trbrdrt\brdrs\brdrw10 =
\trbrdrl\brdrs\brdrw10 \trbrdrb\brdrs\brdrw10 \trbrdrr\brdrs\brdrw10 =
\trbrdrh
\brdrs\brdrw10 \trbrdrv\brdrs\brdrw10 =
\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3 =
\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx2973\clvertalt
\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 \clbrdrb\brdrs\brdrw10 =
\clbrdrr\brdrs\brdrw10 \cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx6054\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10=20
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx9135\row }\trowd =
\trgaph108\trleft-108\trbrdrt\brdrs\brdrw10 \trbrdrl\brdrs\brdrw10 =
\trbrdrb\brdrs\brdrw10 \trbrdrr\brdrs\brdrw10 \trbrdrh\brdrs\brdrw10 =
\trbrdrv\brdrs\brdrw10=20
\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3 =
\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx2973\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl
\brdrs\brdrw10 \clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 =
\cellx6054\clvertalt\clbrdrt\brdrs\brdrw10 \clbrdrl\brdrs\brdrw10 =
\clbrdrb\brdrs\brdrw10 \clbrdrr\brdrs\brdrw10 =
\cltxlrtb\clftsWidth3\clwWidth3081 \cellx9135
\pard \ql \li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 =
{\f1\cgrid0 ( )Yellow fever virus}{\b\f1\fs22\cgrid0 \cell \cell \cell =
}\pard \ql \li0\ri0\widctlpar\intbl\faauto\adjustright\rin0\lin0 =
{\b\f1\fs22\cgrid0 \trowd \trgaph108\trleft-108\trbrdrt
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}\pard \ql \li0\ri0\widctlpar\faauto\adjustright\rin0\lin0\itap0 =
{\f1\cgrid0=20
\par }{\b\f1\fs22\cgrid0 Recombinant organisms/molecules
\par }{\f1\fs22\cgrid0 (}{\b\f1\fs22\cgrid0 xx}{\f1\fs22\cgrid0 ) =
Genetically modified microorganisms or genetic elements from organisms =
on Appendix A, shown to produce or encode for a factor associated with a =
disease.
\par (}{\b\f1\fs22\cgrid0 xx}{\f1\fs22\cgrid0 ) Genetically modified =
microorganisms or genetic elements that contain nucleic acid =
sequences}{\b\f1\fs22\cgrid0 }{\f1\fs22\cgrid0 coding for any of the =
toxins listed in this Appendix, or their toxic subunits.
\par=20
\par }{\b\i\f1\fs22\cgrid0 Calculation of Registration Fee
\par }{\f1\fs22\cgrid0=20
\par }{\b\f1\fs22\cgrid0 The Registration fee has been rescinded so no =
calculation or payment has been completed.
\par=20
\par }{\f1\fs22\cgrid0 Refer to Federal Register Notice - =
}{\i\f1\fs22\cgrid0 Notice of Site Registration Fee Schedule... =
}{\f1\fs22\cgrid0=20
(included as an attachment to this Application Package) for information =
on user fees and for definitions of small, medium and large facilities.
\par=20
\par This application is for (mark one):
\par=20
\par \tab ( ) a small facility\tab ($13,000)\line \tab ( ) a medium =
facility\tab ($14,000)\tab C3
\par \tab \tab \tab m"\line \tab ( ) a large facility\tab ($15,000)\tab =
~ ~o
\par Additional charges (mark all that apply):\tab \tab ~'\line \tab ( ) =
facility includes one or more BSL4 laboratories\tab ($2,000)
\par \tab ( ) facility expects to do >50 select agent transfers per =
year\tab ($1,000)
\par=20
\par Total registration fee for this facility: $
\par=20
\par This fee is for the three year registration period.
\par Payment is to be made to Centers for Disease Control and =
Prevention.
\par Payment must accompany Application Package.
\par=20
\par }{\b\i\f1\fs22\cgrid0 Certification and Signature
\par=20
\par }{\f1\fs22\cgrid0 I certify th
at I have been designated as the responsible facility official for the =
institution/organization listed above, and that the information supplied =
in this registration package is, to the best of my knowledge, accurate =
and truthful.
\par=20
\par=20
\par Clifford W. Bond
\par (Signature)\tab \tab \tab \tab (Date)
\par=20
\par Per 42 CFR 72.7 - Penalties
\par=20
\par Individuals in violation of this part are subject to a fine of no =
more than $250,000 or one year in jail, or both. Violations by =
organizations are subject to a fine of no more than $500,000 per event. =
A false, fictitious or fra
udulent statement or representation on the government forms required in =
the part for registration of facilities or for transfers of select =
agents is subject to a fine or imprisonment for not more than five =
years, or both for an individual; and a fine for=20
an organization.
\par=20
\par Send completed application package to: Centers for Disease Control =
and Prevention, Financial Management Office, Debt Management and =
Property Control Division, 1600 Clifton Road, Mailstop D48, Atlanta, GA =
30333
\par }}
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\ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 =
\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {\b\f1\fs28 NIH =
Biosafety Level 3 (BL3N) (Large Animals)
\par }{\f1\fs22=20
\par }{\b\f1\fs22 These questions are based on the Biosafety Level 3 =
(BL3N) section of the }{\b\i\f1\fs22 NIH Guidelines for Research =
Involving Recombinant DNA Molecules, }{\b\f1\fs22 March 1996 edition, =
Appendix Q-I, Q-III and Q-II-C.
\par=20
\par }\pard \qj \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\b\f1\fs22 =
Complete this section only if your laboratory works with large animal =
species (e.g., cattle, swine, sheep, goats, horses, poultry) of size
or growth requirements that preclude use of laboratory animal =
containment facilities.
\par }\pard \ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\b\f1\fs22=20
\par Please circle the response that best describes the facility In =
which work with select agents
\par will be carried out.
\par=20
\par N.A. =3D not applicable. If you mark "N.A.", please provide a brief =
explanation below that
\par item or on a separate page.
\par=20
\par }{\f1\fs22 Appendix Q-I. General Considerations
\par=20
\par Appendix Q-I-A. Containment Levels
\par=20
\par }\pard\plain \s15\ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 =
\f1\fs22\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {Yes, No, =
N.A. The containment levels required for research involving recombinant =
DNA associated with or in animals is=20
based on classification of experiments in Section III, Experiments =
Covered by the NIH Guidelines.
\par }\pard\plain \ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 =
\fs24\lang1033\langfe1033\cgrid\langnp1033\langfenp1033 {\f1\fs22=20
\par Appendix Q-I-B. Disposal of Animals (BL1-N through BL4-N)
\par=20
\par Yes, No, N.A. Appendix Q-I-B-1. When an animal covered by Appendix =
Q containing recombinant DNA o
r a recombinant DNA-derived organism is euthanized or dies, the carcass =
shall be disposed of to avoid its use as food for human beings or =
animals unless food use is specifically authorized by an appropriate =
Federal agency,
\par Yes, No, N.A. Appendix Q-I-B-2. A permanent record shall be =
maintained of the experimental use and disposal of each animal or group =
of animals.
\par }\pard \qr \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22 C~
\par }\pard \ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22 =
Appendix Q-III. Footnotes and References for Appendix Q
\par=20
\par Yes, No, N,A, Appendix Q-III-B. Personnel who handle pathogenic and =
potentia
lly lethal agents shall be required to have specific training and be =
supervised by knowledgeable scientists who are experienced in working =
with these agents. BL3-N containment also minimizes escape of =
recombinant DNA-containing organisms from exhaust air=20
or waste material from the containment area.
\par=20
\par Yes, No, N.A. Within work areas of the animal facility, all =
activities shall be confined to the specially equipped animal rooms or =
support areas.
\par=20
\par Appendix Q-II-C-1. Standard Practices (BL3-N)
\par=20
\par Appendix Q-II-C-l-a. Animal Facility Access (BL3-N)
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-a-(1 ). The containment area shall =
be locked.
\par=20
\par Yes, No, N,A. Appendix Q-II-C-l-a-(2), The containment area shall =
be patrolled or monitored at frequent intervals.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-a-(3), The containment building =
shall be controlled and have a locking access.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-a-(4). The Animal Facility Director =
shall establish policies and procedures whereby only persons who have =
been advised of the potential hazard and who meet any specific entry =
requirements=20
(e,g., vaccination) shall enter the laboratory or animal rooms.
\par=20
\par }\pard \qj \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22 =
Yes, No, N.A. Appendix Q-Il-C-l-a-(5), Animal room doors, gates, or =
other closures shaft be kept closed when experiments are in progress,
\par }\pard \ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22=20
\par Appendix Q-II-C-I-b. Decontamination and Inactivation (BL3-N)
\par=20
\par Yes, No, N.A. Appendix Q-Il-C-1-b-(l). The work surfaces of =
containment equipment shall be decontaminated when work with organisms =
containing rec
ombinant DNA molecules is finished. Where feasible, plastic-backed paper =
toweling shall be used on nonporous work surfaces to facilitate =
clean-up.
\par=20
\par }\pard \qj \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22 =
Yes, No, N.A. Appendix Q-II-C-1-b-(2). All animals shall be euthanized =
at the end of their experimental usefulness and the carcasses =
decontaminated before disposal in an approved manner.
\par }\pard \ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22=20
\par }\pard \qj \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22 =
Yes, No, N.A, Appendix Q-Il-C-l-b-(3). Needles and syringes shall be =
promptly placed in a puncture-resistant container and decontaminated, =
preferably by autoclaving, before discard or reuse.
\par }\pard \ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22=20
\par }\pard \qj \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22 =
Yes, No, N.A. Appendix Q-II-C-l-b-(4). Special safety testing, =
decontamination procedures, and Institutional Biosafety Committee app
roval shall be required to transfer agents or tissue/organ specimens =
from a BL3-N animal facility to a facility with a lower containment =
classification.
\par }\pard \ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22=20
\par }\pard \qj \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22 =
Yes, No, N.A. Appendix Q-Ti-C-l-b-(5). Liquid effluent from containment =
equipment, sinks, biological s
afety cabinets, animal rooms, primary barriers, floor drains, and =
sterilizers shall be decontaminated by heat treatment before being =
released into the sanitary system. The procedure used for heat =
decontamination of liquid wastes shall be monitored with a=20
recording thermometer. The effectiveness of the heat decontamination =
process system shall be revalidated every 30 days with an indicator =
organism.
\par }\pard \ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22=20
\par Appendix Q-II-C-I-c. Signs (BL3-N)
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-c-(1). When the animal research =
requires=20
special provisions for entry (e.g., vaccination), a warning sign =
incorporating the universal biosafety symbol shall be posted on all =
access doors to the animal work area. The sign shall indicate: (i
) the agent, (ii) the animal species, (iii) the name and telephone =
number of the Animal Facility Director or other responsible individual, =
and (iv) any special requirements for entering the laboratory.
\par=20
\par Appendix Q-II-C-I-d. Protective Clothing (BL3-N)
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-d-(1). Full protective clothi
ng that protects the individual (e.g., scrub suits, coveralls, uniforms) =
shall be worn in the animal area. Clothing shall not be worn outside the =
animal containment area and shall be decontaminated before laundering or =
disposal, Personnel shall be require
d to shower before exiting the BL3-N area and wearing of personal =
clothing.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-d-(2). Special care shall be taken =
to avoid skin contamination with microorganisms containing recombinant =
DNA. Impervious and/or protective
gloves shall be worn when handling experimental animals and when skin =
contact with an infectious agent is unavoidable.
\par=20
\par }\pard \qj \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22 =
Yes, No, N.A, Appendix Q-Il-C-l-d-(3). Appropriate respiratory =
protection shall be worn in rooms containing experimental animals.
\par }\pard \ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22=20
\par Appendix Q-II-C-I-e. Records (BL3-N)
\par=20
\par }\pard \qj \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22 =
Yes, No, N.A. Appendix Q-II-C-l-e-(1). Documents regarding experimental =
animal use and disposal shall be maintained in a permanent record book.
\par }\pard \ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22=20
\par Yes, No, N.A. Appendix Q.II-C-I-e-(2). Any incident involving =
spills and accidents that result in environmental release or exposure of =
animals or laboratory workers to organisms containing recombinant DNA =
shall be reported immediately to the Biological Sa
fety Office, Animal Facility Director, and other appropriate =
authorities. Medical evaluation, surveillance, and treatment shall be =
provided as appropriate and written records maintained. If necessary, =
the area shall be appropriately decontaminated.
\par=20
\par Yes, No, N.A, Appendix Q-Il-C-l-e-(3). When appropriate and giving =
consideration to the agent handled, baseline serum samples shall be =
collected and stored for animal care and other at-risk personnel. =
Additional serum specimens may be collected periodical
ly depending on the agent handled or the function of the facility.
\par=20
\par Appendix Q-II-C-I-f. Transfer of Materials (BL3-N)
\par=20
\par }\pard \qj \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22 =
Yes, No, N.A. Appendix Q-II-C-l-f-(1). Biological materials removed from =
the animal containment laboratory in a viable or intact state sh
all bo transferred to a non-breakable sealed primary container and then =
enclosed in a non-breakable sealed secondary container. All containers, =
primary and secondary, shall be disinfected before removal from the =
animal facility. Advance approval for trans
f
er of material shall be obtained from the Animal Facility Director. =
Packages containing viable agents may be opened only in a facility =
having an equivalent or higher level of physical containment unless the =
agent is biologically inactivated or incapable o
f reproduction.
\par }\pard \ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22=20
\par Yes, No, N.A. Appendix Q-II-C-l-f-(2). Special safety testing, =
decontamination procedures, and Institutional Biosafety Committee =
approval shall be required to transfer agents or tissue/organ specimens =
from a BL3-N animal facility to a facil
ity with a lower containment classification.
\par=20
\par Appendix Q-II-C-I-g. Other (BL3-N)
\par=20
\par }\pard \qj \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22 =
Yes, No, N.A. Appendix Q-II-C-l-g-(1). All genetically engineered =
neonates shall be permanently marked within 72 hours after birth, if =
their size permits. If their size does=20
not permit marking, their containers should be marked. In addition, =
transgenic animals should contain distinct and biochemically assayable =
DNA sequences that allow identification of transgenic animals from among =
non-transgenic animals.
\par }\pard \ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22=20
\par Yes, No, N.A. Appen
dix Q-Il-C-l-g-(2). Appropriate steps should be taken to prevent =
horizontal transmission or exposure of laboratory personnel. If the =
agent used as the vector is known to be transmitted by a particular =
route (e.g., arthropods), special attention should be=20
given to preventing spread by that route. In the absence of specific =
knowledge of a particular route of transmission, all potential means of =
horizontal transmission (e.g., arthropods, contaminated bedding, or =
animal waste) should be prevented.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-g-(3). Eating, drinking, smoking, =
and applying cosmetics shall not be permitted in the work area.
\par Yes, No, N.A. Appendix Q-II-C-l-g-(4). Individuals who handle =
materials and animals containing recombinant DNA molecules shall be =
required to wash their hands before exiting the containment area.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-g-(5). Experiments involving other =
organisms that require containment levels lower than BL3-N may be =
conducted in the same area concurrently with experiments requiring BL3-N =
containment provided that they are conducted in accordance with BL3-N =
practices.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-g-(6). Animal holding areas shall =
be cleaned at least once a day and decontaminated immediately following =
any spill of viable materials.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-g-(7). All procedures shall be =
performed carefully to minimize the creation of aerosols.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-g-(8). A double barrier shall be =
provided to separate male and female animals unless reproductive studies =
are
part of the experiment or other measures are taken to avoid =
reproductive transmission. Reproductive incapacitation may be used.
\par=20
\par Yes, No, N.A. Appendix Q-Il-C-l-g-(9). The containment area shall =
be in accordance with state and Federal laws and animal care =
requirements.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-g-(10). All animals shall be =
euthanized at the end of their experimental usefulness and the carcasses =
decontaminated before disposal in an approved manner.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-g-(11 ). Personnel shall be =
required to shower before exiting the BL3-N area and wearing personal =
clothing.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-g-(12). Animals of the same or =
different species, which are not involved in the work being performed, =
shall not be permitted in the animal area,
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-g-(13), Needles and syringes shall =
be used only for parenteral injection and aspiration of fluids from =
laboratory animals and diaphragm b
ottles, Only needle-looking syringes or disposable syringe-needle units =
(i.e., needle is integral to the syringe) shall be used for the =
injection or aspiration of fluids containing organisms that contain =
recombinant DNA. Extreme caution shall be used when
=20
handling needles and syringes to avoid autoinoculation and the =
generation of aerosols during use and disposal, Following use, needles =
shall not be bent, sheared, replaced in the needle sheath or guard or =
removed from the syringe. The needles and syringes=20
shall be promptly placed in a puncture-resistant container and =
decontaminated, preferably by autoclaving, before discard or reuse.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-l-g-(14). A biosafety manual shall be =
prepared or adopted. Personnel shall be advised of special hazards and =
required to read and follow instructions on practices and procedures.
\par=20
\par Appendix Q-II-C-2. Animal Facilities (BL3-N)
\par=20
\par Yes, No, N,A. Appendix Q-II-C-2-a. Animals shall be contained =
within an enclosed structure (animal room or equivalent) to min
imize the possibility of theft or unintentional release and avoid =
arthropod access. The special provision to avoid the entry or escape of =
arthropods from the animal areas may be waived if the agent in use is =
not known to be transmitted by arthropods.
\par=20
\par Yes,
No, N.A. Appendix Q-II-C-2-b. The interior walls, floors, and ceilings =
shall be impervious to water and resistant to acids, alkalis, organic =
solvents, and moderate heat, to facilitate cleaning. Penetrations in =
these structures and surfaces (e.g., plumbin
g and utilities) shall be sealed.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-2-c. Windows in the animal facility =
shall be closed, sealed, and breakage resistant (e.g., double-pane =
tempered glass or equivalent). The need to maintain negative pressure =
should be considere
d when constructing or renovating the animal facility.
\par=20
\par }\pard \qj \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22=20
Yes, No, N.A. Appendix Q-II-C-2-d, An autoclave, incinerator, or other =
effective means to decontaminate animals and waste shall be available, =
preferably within the containment area, If feasible, a dou
ble-door autoclave is preferred and should be positioned to allow =
removal of material from the containment area.
\par }\pard \ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22=20
\par Yes, No, N.A. Appendix Q-II-C-2-e. If arthropods are used in the =
experiment or the agent under study can be transmitted by an arthropod, =
the interior work area shall be appropriately screened (52 mesh). All =
perimeter joints and openings shall be sealed, a
nd additional arthropod control mechanisms used to minimize arthropod =
entry and propagation, including appropriate screening, or the =
equivalent of access doors.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-2-f. Access doors to the containment =
area shall be self-closing.
\par=20
\par Yes, No, N.A, Appendix Q-II-C-2-g. The animal area shall be =
separated from all other areas. Passage through two sets of doors shall =
be the basic requirement for entry into the animal area from access =
corridors or other contiguous areas, The anima
l containment area shall be physically separated from access corridors =
and other laboratories or areas by a double-door clothes change room, =
equipped with integral showers and airlock.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-2-h, Liquid effluent from containment =
eq
uipment, sinks, biological safety cabinets, animal rooms, primary =
barriers, floor drains, and sterilizers shall be decontaminated by heat =
treatment before being released into the sanitary system. The procedure =
used for heat decontamination of liquid waste
s shall be monitored with a recording thermometer. The effectiveness of =
the heat decontamination process system shall be revalidated every 30 =
days with an indicator organism.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-2-i. An exhaust air ventilation =
system shall be pro
vided. This system shall create directional airflow that draws air into =
the animal room through the entry area. The building exhaust, or the =
exhaust from primary containment units, may be used for this purpose if =
the exhaust air is discharged to the outsi
de and shall be dispersed away from occupied areas and air intakes. =
Personnel shall verify that the direction of the airflow (into the =
animal room) is proper.
\par=20
\par }\pard \qj \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22 =
Yes, No, N.A. Appendix Q-II-C-2-j. If the agent is transmitted by =
aerosol, then the exhaust air shall pass through a high efficiency =
particulate air/HEPA filter.
\par }\pard \ql \li0\ri0\nowidctlpar\faauto\rin0\lin0\itap0 {\f1\fs22=20
\par Yes, No, N.A. Appendix Q-II-C-2-k. Vacuum lines shall be protected =
with high efficiency particulate air/HEPA filters and liquid =
disinfectant traps.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-2-l. In lieu of open housing in the =
special animal room, animals held in a BL3-N area may be housed in parti
al-containment caging systems (e.g., Horsfall units or gnotobiotic =
systems, or other special containment primary barriers). Prudent =
judgment must be exercised to implement this ventilation system (e.g., =
animal species) and its discharge location.
\par=20
\par Yes, No, N.A. Appendix Q-II-C-2-m. Each animal area shall contain a =
foot, elbow, or automatically operated sink for hand washing. The sink =
shall be located near the exit door.
\par=20
\par Yes, No, N.A, Appendix Q-II-C-2-n. Restraining devices for animals =
may be required to avoid damage to the integrity of the animal =
containment facility.
\par }}
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------=_NextPart_000_0048_01BF2CE8.4E199F00--
=========================================================================
Date: Fri, 12 Nov 1999 10:35:56 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Petty, Carol"
Subject: Re: CDC Laboratory Registration (Select Agent Transfer)
MIME-Version: 1.0
Content-Type: text/plain
Thank you Clifford. You have really helped clarify what needs to be filled
out. Thanks very much.
Carol L. Petty, C.I.H.
Industrial Hygienist
Phone: (505) 845-1076
Fax: (505) 845-1174
email: cpetty@
> -----Original Message-----
> From: Clifford W. Bond [SMTP:umbcb@GEMINI.OSCS.MONTANA.EDU]
> Sent: Friday, November 12, 1999 8:31 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Re: CDC Laboratory Registration (Select Agent Transfer)
>
> Carol,
>
> We scanned a bunch of the documents into Word to make it easier to fill
> out
> the forms. The forms are attached. If you need others let me know. A
> contact who may be able to help you is David Bressler at CDC (404
> 639-4418).
> He handled our registration.
>
> Cliff Bond
>
>
> Clifford W. Bond, Professor
> Department of Microbiology
> Montana State University
> Bozeman, MT 59717-3520
> Email: umbcb@gemini.oscs.montana.edu
> Internet:
> Telephone: 406 994-4130
> TeleFAX: 406 994-4926
>
>
> -----Original Message-----
> From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
> Behalf Of Petty, Carol
> Sent: Friday, November 12, 1999 8:47 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: CDC Laboratory Registration (Select Agent Transfer)
> Importance: High
>
>
> Hi!
> Has anyone had to register with the CDC for select agent transfer tracking
> system. We are working with some agents on the list and I requested the
> application kit but did not get the associated forms with it. Does anyone
> have them? Or are they available somewhere on the intranet that I am not
> aware of. Let me know as soon as you can...time pressure. Thanks.
>
> Carol L. Petty, C.I.H.
> Industrial Hygienist
> Phone: (505) 845-1076
> Fax: (505) 845-1174
> email: cpetty@ >
=========================================================================
Date: Fri, 12 Nov 1999 13:28:06 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Hofherr, Leslie"
Subject: Biosafety Position Open -- UC Santa Barbara
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Hi Everyone,
Below is a job posting for the University of California - Santa Barbara
campus.
Sincerely,
Leslie Hofherr
UCLA laboratory and biological safety
Leslie@admin.ucla.edu
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Job Opening: Biosafety Officer for the University of California at Santa
Barbara. Duties: work with campus Biosafety Committee on authorizations;
Employee training; Develop written program elements;
Bloodborne pathogens program; Medical waste program; Lab inspections;
Biosafety cabinets. Also assist with Environmental Health programs:
food safety; pool safety; reclaimed water and storm water.
Minimum requirements: Bachelor's degree in Biology, Biochemistry or related
field. Demonstrated knowledge and experience in the identification and
evaluation of safety hazards related to biological materials. Strong
ability to communicate orally and in writing.
Desirable qualifications: Master's or Bachelor's degree in
Microbiology. Demonstrated knowledge and experience in developing an
institutional biosafety program. Lab research experience in Biology.
Job # 99-11-004
Closing date: 12/1/99
Applications should be directed to:
Human Resources Employment Unit
SAASB, Room 3101
University of California
Santa Barbara, CA 93106-3160
(805) 893-3311
Website: hr.ucsb.edu
----------------------
David Vandenberg
Laboratory Safety Specialist
UCSB Env. Health & Safety
(805) 893-4899
David.Vandenberg@ehs.ucsb.edu
=========================================================================
Date: Mon, 15 Nov 1999 10:21:24 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: OSHA's BBP Directive change
MIME-Version: 1.0
Content-Type: text/plain
> ----------
> From: Civitella Patricia PC
> Sent: Monday, November 15, 1999 10:17 AM
> To: Schlank Bliss BM
> Subject: FYI
> Sensitivity: Private
>
> I got this in a regulatory e-mail:
>
> OSHA REVISES BLOODBORNE PATHOGENS COMPLIANCE DIRECTIVE
>
> A new directive issued by the Occupational Safety and Health
> Administration will help minimize serious health risks faced by
> workers exposed to blood and other potentially infectious
> materials. Among the risks are human immunodeficiency virus
> (HIV), hepatitis B and hepatitis C.
>
> The directive guides OSHA's compliance officers in enforcing the
> standard that covers occupational exposure to bloodborne
> pathogens and ensures consistent inspection procedures are
> followed. It updates an earlier directive issued in 1992 and
> reflects the availability of improved devices, better treatment
> following exposure and OSHA policy interpretations.
>
> The revised directive emphasizes the importance of an annual
> review of the employer's bloodborne pathogens program and the use
> of safer medical devices to help reduce needlesticks and other
> sharps injuries. OSHA does not advocate the use of one particular
> medical device over another. The directive also highlights basic
> work practices, personal protective equipment and administrative
> controls.
>
> The emphasis on engineering controls results from OSHA's request
> last year for ideas and recommendations on ways to better protect
> workers from contaminated needles or other sharp objects.
>
> "We received nearly 400 comments from health care facilities,
> workers and others," said OSHA Administrator Charles N. Jeffress.
> "They told us that safe medical devices already available are
> effective in controlling hazards and that wider use of such
> devices would reduce thousands of injuries each year."
>
> The revised directive also includes detailed instructions to
> compliance officers on inspections of multi-employer worksites,
> such as home health services, employment agencies, personnel
> services, physicians and health care professionals in independent
> practices, and independent contractors.
>
> Also included in the directive are decontamination requirements,
> guidelines on hepatitis vaccinations and post exposure
> treatments, and employee training.
>
> OSHA issued a final regulation on occupational exposure to
> bloodborne pathogens in 1991 to protect nearly six million
> workers in health care and related occupations at risk of
> exposure to bloodborne diseases. Jeffress said the agency will
> review the standard to determine whether its revision is
> warranted.
>
> The directive can be accessed from the OSHA web page at
> .
> Copies can also be obtained from the agency's Publications Office
> by calling (202) 693-1888.
>
>
> Patti Civitella
> Environmental Specialist
> US SHE Services
> (302) 886-3486
> (302) 886-2909 fax
> e-mail: patricia.civitella@phwilm.
>
=========================================================================
Date: Mon, 15 Nov 1999 12:50:58 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph P. Kozlovac"
Subject: Fwd: New C.H.A.S. Certificate Program
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
I thought the group may have some interest in this forwarded message from
the LabSafety discussion list.
>Date: Mon, 15 Nov 1999 11:55:09 +0100
>Reply-To: LABSAFETY-L Discussion List
>Sender: LABSAFETY-L Discussion List
>From: Armen Casparian
>Subject: New C.H.A.S. Certificate Program
>To: LABSAFETY-L@SIU.EDU
>
> Hi NACHOS,
>
> I am in the process of collecting facts and figures to
> support the possible launch of a new certificate program in
> chemical health and safety here at Wentworth Institute of Technology
> in Boston. (Actually, Wentworth is one of six members of the Colleges
> of the Fenway, all of whom will share enrollment in the program.)
> My committee has worked out most of the program's requirements--
> courses, credits, internships, etc.
>
> I still have to make a presentation to our curriculum review
committee
> at the end of this week to essentially sell the program and get
> final approval. I would like to know if anyone has current or
> projected employment figures/trends for health and safety
>positions,
>
> like CHO'S, lab. management personnel, etc. which I might use to
>bolster
> my pitch? The Bureau of Labor Statistics figures are rather
> vague. Any reliable info., along with its source, would be greatly
> appreciated. So too are any random thoughts or opinions on the
program
> itself.
>
> Armen Casparian
>
______________________________________________________________________________
Biological Safety Officer
Safety and Environmental Protection Program
NCI - Frederick Cancer Research
and Development Center
(301)846-1451 fax: (301)846-6619
email: jkozlovac@mail.
______________________________________________________________________________
=========================================================================
Date: Tue, 16 Nov 1999 21:18:44 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Disposal of cells lines
MIME-Version: 1.0
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Disposal of cell lines.
The subject was discussed on the biosafety discussion list a few months ago.
How does one access the archives to determine what the responses were?
Regards,
Ed Krisiunas, MT(ASCP), CIC, MPH
Director
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
=========================================================================
Date: Wed, 17 Nov 1999 13:41:43 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Madeline J. Dalrymple"
Subject: Faucet Elbow Paddles/Handles
MIME-version: 1.0
Content-type: text/plain; charset="iso-8859-1"
A researcher here wants to replace wrist paddles on his lab's water faucets
with "true" elbow paddles.
He hasn't found any in his catalogs.
Do you know of suppliers of elbow handles?
Thank You!
Madeline Dalrymple
Biological Safety Officer
University of Wyoming Environmental Health and Safety Office
Laramie, Wyoming; USA
307-766-2723; dalrympl@uwyo.edu
=========================================================================
Date: Wed, 17 Nov 1999 13:57:09 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Petty, Carol"
Subject: Biosafety 4 Level
MIME-Version: 1.0
Content-Type: text/plain
Our facility is presently undergoing some discussions on developing a
Biosafety 4 facility for animal/agent studies. If any of you out there have
experience in this, I sure would appreciate you letting me know some
insights you have gained through the process. (e.g. what are the weak
links, where were the surprises?, etc.). Thank you.
Carol L. Petty, C.I.H.
Industrial Hygienist
Phone: (505) 845-1076
Fax: (505) 845-1174
email: cpetty@
=========================================================================
Date: Wed, 17 Nov 1999 15:07:52 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Edwin Jackson
Subject: Re: Biosafety 4 Level
Mime-Version: 1.0
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This is a MIME message. If you are reading this text, you may want to
consider changing to a mail reader or gateway that understands how to
properly handle MIME multipart messages.
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There are not a lot of true BSL4 facilities around. I think that it
would be worth your time to visit the facilities at CDC and USAMRIID.
A lot depends on whether you are going to isolate the investigators
with space suits or glove boxes. While I was at USAMRIID one of the
investigators got a hole in his space suit while working with Lassa.
This resulted in isolation for a few weeks. I would guess that work
in a glove box would be easier to control. But some work is
impossible in that confined area.
Address: 122 TOMH BYU
Provo, UT 84602-0100
Telephone: (801) 378-5779
Fax (801 378-5977
e-mail edwin_jackson@byu.edu
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EMAIL;WORK;PREF;NGW:EDWIN
N:Jackson;Edwin
X-GWUSERID:EDWIN
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=========================================================================
Date: Thu, 18 Nov 1999 13:17:51 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Home
Subject: Re: Faucet Elbow Paddles/Handles
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
I am thinking you mean the devices used in scrub rooms or clinical settings.
I suppose you could try a plumbing supply dealer who has provided them to
hospitals or clinics - or check with the hospital/clinic maintenance
department.
Dan Lessley, CIH, CSP
dlessley@
-----Original Message-----
From: Madeline J. Dalrymple
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Wednesday, November 17, 1999 3:04 PM
Subject: Faucet Elbow Paddles/Handles
>A researcher here wants to replace wrist paddles on his lab's water faucets
>with "true" elbow paddles.
>He hasn't found any in his catalogs.
>Do you know of suppliers of elbow handles?
>
>Thank You!
>
>Madeline Dalrymple
>Biological Safety Officer
>University of Wyoming Environmental Health and Safety Office
>Laramie, Wyoming; USA
>307-766-2723; dalrympl@uwyo.edu
>
=========================================================================
Date: Thu, 18 Nov 1999 14:46:39 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Biosafety 4 Level
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
At 01:57 PM 11/17/99 -0700, Carol Petty wrote:
>Our facility is presently undergoing some discussions on developing a
>Biosafety 4 facility for animal/agent studies. If any of you out there have
>experience in this, I sure would appreciate you letting me know some
>insights you have gained through the process. (e.g. what are the weak
>links, where were the surprises?, etc.). Thank you.
If you really mean BL4, I would talk with Health Canada (they opened a level 4
facility fairly recently) and the US CDC.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Thu, 18 Nov 1999 15:30:47 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Elizabeth Smith
Subject: microbiological claims
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
This concerns the utilization of non-pathogenic bacteria - a particular
strain of an organism is missing part of its structure which is required to
cause disease. (Sorry, I don't know exactly what is missing...)
In discussing required safety measures with a fellow employee, a claim was
made by the individual that a particular safety practice (that is more
stringent than I thought necessary) had been originally employed because it
was claimed/concerned the organisms would suddenly "revert to their wild
state and become pathogenic again".
My question is: given microorganisms with no interaction with the outside
world, can bacteria just "switch" from one strain to another - when it would
require genesis of cellular structures that don't currently exist?
As I understand, the specific bacteria in question are missing a plasmid(?)
which is necessary for pathogenesis. If it isn't there, how's a cell going
to just suddenly decide "hey, I'll produce something for which I have no DNA
to build?"
If there is a short answer, I would appreciate it.
If there is a long answer, a reference to a good source for the answer would
also be appreciated.
And, if it's pretty boring and wouldn't interest any one else, please feel
free to respond to me directly at: safety_queen@
Thank you in advance!
Elizabeth E. Smith
Environmental, Health & Safety Manager
BioPort Corporation
Lansing, Michigan
517-327-6806
__________________________________________________
Do You Yahoo!?
Bid and sell for free at
=========================================================================
Date: Thu, 18 Nov 1999 16:22:16 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Yaw A. Nsiah"
Subject: Re: microbiological claims
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Hello Ms. Smith
Pathogenicity is the ability to cause disease. So there are pathogenic
microbes like streptococcus pyogenes(gp A strep) and non-pathogenic
microbes like staphylococcus epidermidis. The pathogenis ones are grouped
into primary pathogens with ability to cause disease directly without help
from any source; and of course you have the secondary or opportunistic
pathogens which are able to cause disease in the immunocompromised,
debilitated individuals or otherwise not healthy individuals. Pathogenic
organic organisms can become virulent(degree of pathogenicity) by
acquisition of plasmid. There is no in between with virulence... you are
either virulent or you are not.. avirulent. So you see you cannot be
virulent if you cannot cause disease.
Hope this helps.
At 03:30 PM 11/18/99 -0500, you wrote:
>This concerns the utilization of non-pathogenic bacteria - a particular
>strain of an organism is missing part of its structure which is required to
>cause disease. (Sorry, I don't know exactly what is missing...)
>
>In discussing required safety measures with a fellow employee, a claim was
>made by the individual that a particular safety practice (that is more
>stringent than I thought necessary) had been originally employed because it
>was claimed/concerned the organisms would suddenly "revert to their wild
>state and become pathogenic again".
>
>My question is: given microorganisms with no interaction with the outside
>world, can bacteria just "switch" from one strain to another - when it would
>require genesis of cellular structures that don't currently exist?
>
>As I understand, the specific bacteria in question are missing a plasmid(?)
>which is necessary for pathogenesis. If it isn't there, how's a cell going
>to just suddenly decide "hey, I'll produce something for which I have no DNA
>to build?"
>
>If there is a short answer, I would appreciate it.
>
>If there is a long answer, a reference to a good source for the answer would
>also be appreciated.
>
>And, if it's pretty boring and wouldn't interest any one else, please feel
>free to respond to me directly at: safety_queen@
>
>Thank you in advance!
>
>
>Elizabeth E. Smith
>Environmental, Health & Safety Manager
>BioPort Corporation
>Lansing, Michigan
>517-327-6806
>
>
>__________________________________________________
>Do You Yahoo!?
>Bid and sell for free at
>
Yaw A. Nsiah, Ph.D
Assistant Professor (Microbiology)
Eastern Connecticut State Univeristy
Department of Biology, M230
83 Windham Street,
Willimantic, Ct. 06226
Tel: 1-860-465-4524/4488
e-mail: Nsiah@ecsuc.ctstateu.edu
=========================================================================
Date: Thu, 18 Nov 1999 18:05:45 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Larry Thompson
Subject: CISWI's
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boundary="============_-1269156118==_============"
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AAVLD listserve, BIOSAFTY listserve, STAATT folks and others,
=46or your information, the U.S. Environmental Protection Agency's (EPA's)
proposed rule on air emissions from commercial and industrial solid waste
incineration units (CISWI) was signed by the EPA Administrator on November
15, 1999 and was in the Federal Register November 18, 1999.
This proposed rule states several times that
"Incineration units that burn more than 90 percent by
weight (on an instantaneous basis) pathological waste or
agricultural waste (as defined in =A7=A760.2245 and 60.2850) are
not covered by the proposed standards and guidelines."
Bottom line: Institutions with pathological waste incinerators that burn
over 90% pathological waste each load are not covered by this proposed
rule. If they burn under 90% path waste they are either covered by this
proposed rule (CISWI) or come under the HMIWI rules for medical waste
incinerators (depending on what the 10+% non-path waste is...). From the
proposed rule:
"Pathological waste means waste material consisting of
only human or animal remains, anatomical parts, and/or
tissue, the bags/containers used to collect and transport
the waste material, and animal bedding (if applicable)."
Timetable for implementation of this rule will be somewhat up to your
state, but sooner or later we will all live under this. The entire rule
can be found on the Internet at ...
and click on the "Commercial and
Industrial Solid Waste Incineration Units " icons.
I am attaching the small (24k) EPA fact sheet in Acrobat format for your
information. The EPA will cover pathological waste incinerators under
subsequent rules. Stay tuned...
TTFN,
Larry
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AAVLD listserve, BIOSAFTY listserve, STAATT folks and others,
=46or your information, the U.S. Environmental Protection Agency's
(EPA's) proposed rule on air emissions from commercial and industrial
solid waste incineration units (CISWI) was signed by the EPA
Administrator on November 15, 1999 and was in the Federal Register
November 18, 1999.
This proposed rule states several times that
FFFF,0000,0000 "Incineration units that burn more
than 90 percent by
weight (on an instantaneous basis) pathological waste or
agricultural waste (as defined in =A7=A760.2245 and 60.2850) are
not covered by the proposed standards and guidelines."
Bottom line: Institutions with pathological waste incinerators
that burn over 90% pathological waste each
load are not covered by this proposed rule.=20
If they burn under 90% path waste they are either covered by this
proposed rule (CISWI) or come under the HMIWI rules for medical waste
incinerators (depending on what the 10+% non-path waste is...). From
the proposed rule:
FFFF,0000,0000"Pathological waste means waste
material consisting of
only human or animal remains, anatomical parts, and/or
tissue, the bags/containers used to collect and transport
the waste material, and animal bedding (if applicable)."
Timetable for implementation of this rule will be somewhat up
to your state, but sooner or later we will all live under this. The
entire rule can be found on the Internet at ...
and click on the "Commercial
and Industrial Solid Waste Incineration Units " icons.
I am attaching the small (24k) EPA fact sheet in Acrobat format for
your information. The EPA will cover pathological waste incinerators
under subsequent rules. Stay tuned...
TTFN,
Larry
--============_-1269156118==_ma============--
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T0YNCg==
--============_-1269156118==_============
Content-Type: multipart/alternative; boundary="============_-1269156118==_ma============"
--============_-1269156118==_ma============
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: quoted-printable
Larry J. Thompson, DVM PhD
Director of Biosafety
College of Veterinary Medicine
Cornell University
Upper Tower Road phone 607-253-3900
Ithaca, NY 14853-6401 fax 607-253-3943
"There is no limit to the good you can do if you don't care who gets the
credit."
Gen. George Marshall
--============_-1269156118==_ma============
Content-Type: text/enriched; charset="us-ascii"
Content-Transfer-Encoding: quoted-printable
Larry J. Thompson, DVM PhD
Director of Biosafety
College of Veterinary Medicine
Cornell University
Upper Tower Road phone 607-253-3900
Ithaca, NY 14853-6401 fax 607-253-3943
"There is no limit to the good you can do if you don't care who gets=
the credit."
Gen. George Marshall
--============_-1269156118==_ma============--
--============_-1269156118==_============--
=========================================================================
Date: Fri, 19 Nov 1999 10:10:00 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Safety Clip Art
MIME-Version: 1.0
Content-Type: text/plain
Can anyone recommend a location for Biosafety Clip Art?
Thanks!
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Fri, 19 Nov 1999 10:48:02 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Don Callihan
Subject: EPA-approved disinfectants
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
From: Don Callihan@BDX on 11/19/99 10:48 AM
Could someone provide the URL for the list of EPA-approved disinfectants. I
recall someone posting this resource within the last year or so. I have not been
able to find it using several search engines, including EPAs.
Thanks,
Don Callihan
Biosafety Officer
BD Biosciences (Baltimore)
410.773.6684
=========================================================================
Date: Fri, 19 Nov 1999 09:52:07 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: Re: EPA-approved disinfectants
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
The URL is :
Julie A. Johnson, Ph.D.
Biosafety Officer
Environmental Health and Safety
Iowa State University
Ames, IA 50011
e-mail: jajohns@iastate.edu
phone: 515-294-7657
fax: 515-294-9357
web site:
-----Original Message-----
From: Don Callihan [mailto:Don_Callihan@MS.]
Sent: Friday, November 19, 1999 9:48 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: EPA-approved disinfectants
From: Don Callihan@BDX on 11/19/99 10:48 AM
Could someone provide the URL for the list of EPA-approved disinfectants. I
recall someone posting this resource within the last year or so. I have not
been
able to find it using several search engines, including EPAs.
Thanks,
Don Callihan
Biosafety Officer
BD Biosciences (Baltimore)
410.773.6684
=========================================================================
Date: Fri, 19 Nov 1999 11:53:49 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Disposal of cells lines
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
At 09:18 PM 11/16/99 -0500, you wrote:
>Disposal of cell lines.
>
>The subject was discussed on the biosafety discussion list a few months ago.
>
>How does one access the archives to determine what the responses were?
>
To get the archives:
First send the following message to LISTSERV@MITVMA.MIT.EDU
IND BIOSAFTY
The LISTSERV will send back to you the following:
* BIOSAFTY FILELIST for LISTSERV@MITVMA.
*
* Archives for list BIOSAFTY (A Biosafety Discussion List)
*
*
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
::::::::
* The GET/PUT authorization codes shown with each file entry describe
* who is authorized to GET or PUT the file:
*
* ALL = Everybody
* OWN = List owners
*
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
::::::::
*
* NOTEBOOK archives for the list
* (Monthly notebook)
* rec last change
* filename filetype GET PUT fm lrecl nrecs
date
time Remarks
*
BIOSAFTY LOG9507 ALL OWN V 73 8 95/07/14
11:04:48 Started on Thu, 14 Jul 1995 11:04:19 EDT
BIOSAFTY LOG9508 ALL OWN V 80 964 95/08/30
17:03:05 Started on Mon, 1 Aug 1995 14:56:57 EDT
BIOSAFTY LOG9509 ALL OWN V 76 54 95/09/12
07:52:11 Started on Sun, 11 Sep 1995 12:56:50 0500
You can then use the GET command to receive each log file, i.e. send the
following message to LISTSERV@MITVMA.MIT.EDU
GET BIOSAFTY LOG9507
GET BIOSAFTY LOG9508
GET BIOSAFTY LOG9509
This will get you all three log files.
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
=========================================================================
Date: Fri, 19 Nov 1999 12:42:57 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Debra Hunt
Subject: Biological Safety Specialist position
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
The Biological Safety Division of the Duke University Occupational and
Environmental Safety Office has an opening for a Biological Safety Specialist,
who will act in a consulting and advisory role for Duke Medical Center
healthcare workers. Duties include development and coordination of the
institutional biological safety program, department-specific biological safety
audits and policy development, management of the TB exposure control program for
healthcare workers, review and evaluation of occupational exposures to
biological agents and development of applicable preventive measures, and
development and presentation of biological safety training courses. The
Specialist will develop programs to comply with any federal or state regulations
pertaining to biological hazards in the healthcare work environment.
Requirements:
a bachelor's degree in nursing and 3 years of experience in the field of safety
, infection control, or occupational health are required. A graduate degree in
safety management, biological safety, or occupational health preferred. (a
master's degree may be substituted for one year of experience).
This position requires one of the following certifications:
CIC (Certified in Infection Control)
COHN (Certified as an Occupational Health Nurse)
CBSP (Certified as a Biological Safety Professional)
Please inquiries to:
Debra L. Hunt, DrPH, CBSP
Director, Biological Safety
Duke University
hunt0009@mc.duke.edu
919-684-8822
fax: 919-681-7509
=========================================================================
Date: Fri, 19 Nov 1999 13:10:53 +0500
Reply-To: speaker@ehs.psu.edu
Sender: A Biosafety Discussion List
From: Curt Speaker
Organization: UNIVERSITY SAFETY
Subject: Re: Safety Clip Art
In-Reply-To:
Bliss:
There is some safety clip art at Ralph Stuarts web site at:
it is under the safety graphics section. Pretty basic stuff, though...
Hope this helps...
Curt
Curt Speaker
Biosafety Officer
Penn State University
Environmental Health and Safety
speaker@ehs.psu.edu
^...^
(O_O)
=(Y)=
"""
=========================================================================
Date: Fri, 19 Nov 1999 13:48:54 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: BL3 lab leak testing
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Two questions:
1. We're having leaks develop in our BL3 labs around the edge of the autoclave
pass-throughs. Do any of you know of a standard or standard method that
specifies how and how often, to test for leaks in BL3 labs? We need an SOP,
some verbage, and a reference in our maintenance contract to address this
problem.
2. Our Biological Safety Cabinet certifier doesn't really like sealing minor
leaks in cabinet HEPA filters with silicon sealant, b/c they say, that the
filter eventually gets clogged with sealant. True - but the only other option I
can figure is to replace the filter, even when the leak is minor ---> an
expensive alternative. Has anyone heard of another method to stop up minor
leaks in HEPAs that works well?
Thanks for your help in advance and have a HAPPY TURKEY DAY!
Judy Pointer, MS, CBSP
Biosafety Officer
EH&S
UT MD Anderson Cancer Center
jpointer@
fax: 713 745-2025
=========================================================================
Date: Fri, 19 Nov 1999 15:48:26 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Greg Merkle
Organization: Wright State University
Subject: Re: BL3 lab leak testing
MIME-version: 1.0
Content-type: text/plain; charset=us-ascii
Content-transfer-encoding: 7bit
Judy,
There should not be a concern of the HEPA filter being
eventually being clogged with sealant. The NSF Standard 49,
4.19.6, states that the "HEPA filter patches shall not
exceed 5% of the total face area. The maximum width of any
one patch shall not exceed 4.0 cm (1.5 in.)". Anything
exceeding the statement in the NSF guide I would be looking
at replacing.
How "minor" of a filter leak is not being sealed? If the
aerosol penetration does not exceed 0.01 percent during the
testing then I would not believe that sealing the leak would
necessary. I would document these locations of minimal
leakage and monitor for an indication of deterioration.
However, when in doubt it is always easier to deconaminate
the BSC and replace the filter(s).
Greg Merkle
Judy Pointer wrote:
>
> Two questions:
>
> 1. We're having leaks develop in our BL3 labs around the edge of the autoclave
> pass-throughs. Do any of you know of a standard or standard method that
> specifies how and how often, to test for leaks in BL3 labs? We need an SOP,
> some verbage, and a reference in our maintenance contract to address this
> problem.
>
> 2. Our Biological Safety Cabinet certifier doesn't really like sealing minor
> leaks in cabinet HEPA filters with silicon sealant, b/c they say, that the
> filter eventually gets clogged with sealant. True - but the only other option I
> can figure is to replace the filter, even when the leak is minor ---> an
> expensive alternative. Has anyone heard of another method to stop up minor
> leaks in HEPAs that works well?
>
> Thanks for your help in advance and have a HAPPY TURKEY DAY!
>
> Judy Pointer, MS, CBSP
> Biosafety Officer
> EH&S
> UT MD Anderson Cancer Center
> jpointer@
> fax: 713 745-2025
=========================================================================
Date: Fri, 19 Nov 1999 16:11:45 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: EPA-approved disinfectants
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Like I said,
EPA mdoesn't know they have these lists out.
Try here:
Bob
>From: Don Callihan@BDX on 11/19/99 10:48 AM
>
>Could someone provide the URL for the list of EPA-approved disinfectants. I
>recall someone posting this resource within the last year or so. I have
>not been
>able to find it using several search engines, including EPAs.
>Thanks,
>Don Callihan
>Biosafety Officer
>BD Biosciences (Baltimore)
>410.773.6684
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Fri, 19 Nov 1999 16:20:29 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Schlank Bliss BM
Subject: Cells grown under CO2 conditions
MIME-Version: 1.0
Content-Type: text/plain
To answer a perplexing question - Why are cells grown under CO2 conditions?
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Fri, 19 Nov 1999 13:34:39 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Wolz, Sarah"
Subject: French press
MIME-Version: 1.0
Content-Type: text/plain
Some of our researchers are bringing a French press to lyse E.coli and
perhaps Pseudomonas aeruginosa cells (to harvest the DNA). One of the
oldest microbiological technologies, and I understand the instrument is much
the way it was 50 years ago!
Questions:
* its my understanding that few, if any, viable organisms make it
through the press. Anyone have any first hand knowledge of this claim?
* What standard precautions are taken when using a French press? It
is too large to fit into a standard biosafety cabinet. The bugs being lysed
are BL1 and 2. We can do this in a smaller, well-ventilated lab, but
wondered what else in done out there in Micro-world.
Thanks for your help!
Sarah Wolz
EH&S
PathoGenesis Corp.
=========================================================================
Date: Fri, 19 Nov 1999 16:45:39 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stefan Wagener
Subject: Re: French press
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
The biggest problem with a French press is the little Teflon ball and the
user. Because of the high pressure, the valve needs to be opened in a very
careful fashion. If the ball is used or inserted inappropriately or the user
is not extremely careful, the soup just shoots out like a squirt gun and can
make a big splash. Make sure they insert the nozzle into a container with
some kind of a splash protection. There will always be cells that are not
lysed. The amount depends on the issues mentioned above.
Hope this helps.
Stefan :-)
Stefan Wagener, Ph.D, CBSP
Michigan State University, ORCBS
C-126 Research Complex Engineering
East Lansing, MI 48824
Phone: (517) 355-6503
Fax: (517) 353-4871
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Wolz, Sarah
Sent: Friday, November 19, 1999 4:35 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: French press
Some of our researchers are bringing a French press to lyse E.coli and
perhaps Pseudomonas aeruginosa cells (to harvest the DNA). One of the
oldest microbiological technologies, and I understand the instrument is much
the way it was 50 years ago!
Questions:
* its my understanding that few, if any, viable organisms make it
through the press. Anyone have any first hand knowledge of this claim?
* What standard precautions are taken when using a French press? It
is too large to fit into a standard biosafety cabinet. The bugs being lysed
are BL1 and 2. We can do this in a smaller, well-ventilated lab, but
wondered what else in done out there in Micro-world.
Thanks for your help!
Sarah Wolz
EH&S
PathoGenesis Corp.
=========================================================================
Date: Fri, 19 Nov 1999 16:55:58 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Michael R. Betlach"
Subject: Re: French press
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I hope your researchers aren't assuming that the material they obtain from
the French press is no longer infectious. My experience with E. coli and
several Pseudomonas species was that disruption was about 90% efficient per
pass. Typically, two passes would suffice to recover intracellular enzymes
and shear the DNA sufficiently so that the material could be pipetted
easily. If air is entrained when the lid is replaced on the cell itself, the
remaining amount of liquid will be forcibly expelled from the cell through
the tubing, causing the tubing to come out of the receptacle for the cells
if the tubing isn't secured in the receiving container. Typically, 'thick'
cell suspensions (20% wet weight to volume or more) are used. Maximum volume
is 40 ml for the large cell. Cell disruption varies in efficiency with
pressure, slow release (as Stefan mentioned), and number of passes, among
other things.
Alkaline lysis methods with chaeotropes such as guanidine HCl are very
efficient with gram-negative bacteria, and provide a means to recover DNA
and inactivate the organisms at the same time. Perhaps these would be
suitable instead of disruption with the press.
Michael Betlach
Biosafety Officer
Promega Corp.
2800 Woods Hollow Rd.
Madison, WI 53711
(608) 274-1181, Ext. 1270
-----Original Message-----
From: Stefan Wagener [mailto:stefan@PILOT.MSU.EDU]
Sent: Friday, November 19, 1999 3:46 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: French press
The biggest problem with a French press is the little Teflon ball and the
user. Because of the high pressure, the valve needs to be opened in a very
careful fashion. If the ball is used or inserted inappropriately or the user
is not extremely careful, the soup just shoots out like a squirt gun and can
make a big splash. Make sure they insert the nozzle into a container with
some kind of a splash protection. There will always be cells that are not
lysed. The amount depends on the issues mentioned above.
Hope this helps.
Stefan :-)
Stefan Wagener, Ph.D, CBSP
Michigan State University, ORCBS
C-126 Research Complex Engineering
East Lansing, MI 48824
Phone: (517) 355-6503
Fax: (517) 353-4871
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Wolz, Sarah
Sent: Friday, November 19, 1999 4:35 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: French press
Some of our researchers are bringing a French press to lyse E.coli and
perhaps Pseudomonas aeruginosa cells (to harvest the DNA). One of the
oldest microbiological technologies, and I understand the instrument is much
the way it was 50 years ago!
Questions:
* its my understanding that few, if any, viable organisms make it
through the press. Anyone have any first hand knowledge of this claim?
* What standard precautions are taken when using a French press? It
is too large to fit into a standard biosafety cabinet. The bugs being lysed
are BL1 and 2. We can do this in a smaller, well-ventilated lab, but
wondered what else in done out there in Micro-world.
Thanks for your help!
Sarah Wolz
EH&S
PathoGenesis Corp.
=========================================================================
Date: Fri, 19 Nov 1999 15:32:51 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: Cells grown under CO2 conditions
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Hi, Bliss -
The major reason for growing cells under CO2 is because the medium in which
they're being cultured is relying on a bicarbonate-based buffer system and
the CO2 helps to maintain the medium at a proper pH. Cells being cultured
in media that rely on pH control by organic, zwitterionic or other buffers
(such as EPPS, HEPES, or MOPS) rather than a biocarbonate-based buffer, do
not require CO2 incubation. That's a very simplistic answer to a much more
complex issue. I'll be happy to go into more detail with you if you wish.
-- Glenn
------------------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
University of California, San Francisco
Voice 415-476-2097
Fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Schlank Bliss BM [mailto:bliss.schlank@PHWILM.]
Sent: Friday, November 19, 1999 1:20 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Cells grown under CO2 conditions
To answer a perplexing question - Why are cells grown under CO2 conditions?
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Fri, 19 Nov 1999 16:16:23 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Clifford W. Bond"
Subject: Re: Cells grown under CO2 conditions
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Bliss,
Mammalian cell media is often buffered with sodium bicarbonate. It turns
out that many cell lines have an absolute requirement for the sodium
bicarbonate for growth. Earle Swim published a paper on this in the 1950s.
The purpose of the carbon dioxide in the atmosphere above the medium is to
maintain the sodium bicarbonate in the medium. Without the carbon dioxide,
the sodium bicarbonate degrades to carbon dioxide (off in the atmosphere)
and sodium hydroxide (soluble). The pH increases and the cells die. Not a
good thing!
Cliff Bond
Clifford W. Bond, Professor
Department of Microbiology
Montana State University
Bozeman, MT 59717-3520
Email: umbcb@gemini.oscs.montana.edu
Internet:
Telephone: 406 994-4130
TeleFAX: 406 994-4926
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Schlank Bliss BM
Sent: Friday, November 19, 1999 2:20 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Cells grown under CO2 conditions
To answer a perplexing question - Why are cells grown under CO2 conditions?
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Fri, 19 Nov 1999 22:26:33 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "George A. Robertson"
Subject: Re: Cells grown under CO2 conditions
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Cell culture media are buffered using a carbonate-bicarbonate system. The
required CO2 concentration is inversely proportional to the amount of bicarb
in the medium formulation. That is why some media, such as RPMI-1640
require 5% CO2 and DMEM-based require 10%. Chemical buffers can be used,
such as HEPES, eliminating the need for high CO2 atmospheres.
George Robertson
----- Original Message -----
From: Schlank Bliss BM
To:
Sent: Friday, November 19, 1999 4:20 PM
Subject: Cells grown under CO2 conditions
To answer a perplexing question - Why are cells grown under CO2 conditions?
Bliss M. Schlank
Biosafety Specialist
AstraZeneca
1800 Concord Pike
Wilmington DE 19850-5437
302.886.2185 Fax: 302.886.2909
bliss.schlank@phwilm.
=========================================================================
Date: Mon, 22 Nov 1999 14:27:50 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Susan Kingston
Subject: human tumor cell lines and immunocompromised animals
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
I would like to know how other facilities handle the situation where human
tumor cell lines are inoculated into nude (or otherwise immunocompromised)
animals.
1. How do you address the issue of silent infections in these animals with
an agent that is not causing cytopathologic effects in the cell line?
2. Is Animal Biosafety Level 2 sufficient for immunocompromised animals
inoculated with human tumor cell lines?
3. Do you require any special testing of the cell line or the animals? If
you do, which agents are you specifically looking for? How do you address
the aerosolization issue of Lymphocytic Choriomeningitis Virus?
Any advice, references, or opinions are greatly appreciated!
Susan Kingston
--------------------------------------------
Susan K. Kingston DVM, MS
Biological Safety Professional
University of Illinois
102 Environmental Health and Safety Building
101 S. Gregory Street
Urbana, IL 61801-3070
(217)244-1939, fax (217)244-6594
email: skingsto@uiuc.edu
--------------------------------------------
=========================================================================
Date: Mon, 22 Nov 1999 23:55:59 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: James Scott
Organization: University of Toronto Botany
Subject: Re: microbiological claims
In-Reply-To:
MIME-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
Elizabeth,
It is certainly possible that attenuated bacterial strains could
take up "promiscuous" plasmids from the environment through
inadvertant transformation, which might restore pathogenicity or
drug resistance.
Under natural conditions, plasmids are transmitted to new bacterial
hosts by a process known as bacterial conjugation. However, the
plasmid vectors in common use for molecular cloning (mostly based on
pMB1 which was originally isolated from a clinical specimen) methods
lack a copy of the gene that is required for conjucation, and are
thus incapable of spontaneously transforming new hosts. However,
some older cloning vectors based on pBR322 may be co-mobilized with
an environmental plasmid. If such a vector were to carry a drug
resistance gene, for instance, this phenotype could be confered upon
an environmental host quite unintentionally with potentially
hazardous results.
As someone who works with these organisms as a researcher, and not
strictly speaking a biosafety professional, I can assure you that the
risk may be small, but it definitely exists.
James
--
This concerns the utilization of non-pathogenic bacteria
- a particular strain of an organism is missing part of
its structure which is required to cause disease.
(Sorry, I don't know exactly what is missing...)
In discussing required safety measures with a fellow
employee, a claim was made by the individual that a
particular safety practice (that is more stringent than
I thought necessary) had been originally employed
because it was claimed/concerned the organisms would
suddenly "revert to their wild state and become
pathogenic again".
My question is: given microorganisms with no
interaction with the outside world, can bacteria just
"switch" from one strain to another - when it would
require genesis of cellular structures that don't
currently exist?
As I understand, the specific bacteria in question are
missing a plasmid(?) which is necessary for
pathogenesis. If it isn't there, how's a cell going to
just suddenly decide "hey, I'll produce something for
which I have no DNA to build?"
If there is a short answer, I would appreciate it.
If there is a long answer, a reference to a good source
for the answer would also be appreciated.
And, if it's pretty boring and wouldn't interest any one
else, please feel free to respond to me directly at:
safety_queen@
Thank you in advance!
Elizabeth E. Smith
Environmental, Health & Safety Manager
BioPort Corporation
Lansing, Michigan
517-327-6806
=========================================================================
Date: Tue, 23 Nov 1999 10:17:51 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Bernholc, Nicole M"
Subject: DOT/IATA other Shipping and Training requirements
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I vaguely remember this discussion a while back. Does anyone know the
training requirements for this? OUr shipping department does not have
anyone really knowledgeable. OUr person who handles the chemical management
system (tracking chemicals and obtaining msds's) answered shipping
questions. She is leaving so we have a gap. SO. I have had DOT hazardous
materials training a while ago. As questions come up I have been calling
manufacturers of the product. When it is a product experimenters have
created, I look for structurally similar compounds and list acordingly.
I went to the IATA site and they say they have retraining requirements every
18 months.
Is there any way to get training on all at once?
Also, on one of the searches I came up with a site that said that a
hazardous goods specialist must be assigned at every location. IS this a
new ammendment to DOT or has this always been a requirement? Or is this
something that the site creator made up?
Thanks for your input.
Nicole M. Bernholc, CIH
Brookhaven National Laboratory
Bld 129
Upton, NY 11973
(516)344-2027
=========================================================================
Date: Tue, 23 Nov 1999 10:34:42 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Neal R. Tempel"
Subject: Re: DOT/IATA other Shipping and Training requirements
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
This is hard to believe. We have such expertise here at BNL. No wonder
everything is so screwed up.
"Bernholc, Nicole M" wrote:
> I vaguely remember this discussion a while back. Does anyone know the
> training requirements for this? OUr shipping department does not have
> anyone really knowledgeable. OUr person who handles the chemical management
> system (tracking chemicals and obtaining msds's) answered shipping
> questions. She is leaving so we have a gap. SO. I have had DOT hazardous
> materials training a while ago. As questions come up I have been calling
> manufacturers of the product. When it is a product experimenters have
> created, I look for structurally similar compounds and list acordingly.
>
> I went to the IATA site and they say they have retraining requirements every
> 18 months.
>
> Is there any way to get training on all at once?
>
> Also, on one of the searches I came up with a site that said that a
> hazardous goods specialist must be assigned at every location. IS this a
> new ammendment to DOT or has this always been a requirement? Or is this
> something that the site creator made up?
>
> Thanks for your input.
>
> Nicole M. Bernholc, CIH
> Brookhaven National Laboratory
> Bld 129
> Upton, NY 11973
> (516)344-2027
=========================================================================
Date: Tue, 23 Nov 1999 10:07:51 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Dianne Fightmaster
Subject: DOT/IATA other Shipping and Training requirements
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
We just recently had Saftpak come in and train 90 people at MD Anderson,
Houston,Tx. They charged us x amount per day with a max amount of people in
class and we charged each department. It was helpful and they are now
"certified shippers". You can find them on the internet at
Dianne Fightmaster
safety specialist
UT- MD Anderson Cancer Center
Houston, Tx
---------------------- Forwarded by Dianne L. Fightmaster/MDACC on 11/23/99
10:07 AM ---------------------------
"Bernholc, Nicole M" on 11/23/99 09:17:51 AM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Dianne L. Fightmaster/MDACC)
Subject: DOT/IATA other Shipping and Training requirements
I vaguely remember this discussion a while back. Does anyone know the
training requirements for this? OUr shipping department does not have
anyone really knowledgeable. OUr person who handles the chemical management
system (tracking chemicals and obtaining msds's) answered shipping
questions. She is leaving so we have a gap. SO. I have had DOT hazardous
materials training a while ago. As questions come up I have been calling
manufacturers of the product. When it is a product experimenters have
created, I look for structurally similar compounds and list acordingly.
I went to the IATA site and they say they have retraining requirements every
18 months.
Is there any way to get training on all at once?
Also, on one of the searches I came up with a site that said that a
hazardous goods specialist must be assigned at every location. IS this a
new ammendment to DOT or has this always been a requirement? Or is this
something that the site creator made up?
Thanks for your input.
Nicole M. Bernholc, CIH
Brookhaven National Laboratory
Bld 129
Upton, NY 11973
(516)344-2027
=========================================================================
Date: Tue, 23 Nov 1999 11:37:03 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Jean.Goldberg"
Subject: Ethidium Bromide Gels
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; CHARSET=US-ASCII
We are trying to decide whether or not to treat gels that
have ethidium bromide in them as hazardous waste. I would
appreciate hearing from others about their rationale for
making a decision on this issue. Thanks in advance.
----------------------------------------
Jean Goldberg
Email: Jean.Goldberg@Med.Nyu.Edu
"NYU Medical Center"
=========================================================================
Date: Tue, 23 Nov 1999 09:08:42 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Al Jin
Subject: Re: microbiological claims
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Elizabeth,
I would also like to add to James's comment that a more feasible
possibility of an accidental conjugation event occurring is when a
researcher is working with 2 different plasmid deficient strains (or 2
researcher working with 2 different deficient strains) in the same
laboratory. Somehow the two strains got mixed and as a result, you now have
a wild-type bacteria.
As an IBC administrative control, if a researcher is planning to work with
2 different deficient strains of the same bug, the researcher is prohibited
in working with both deficient strains at the same time. This would
eliminate any possibility of cross contamination leading to a recombination
event.
Alfred Jin, BSO, IH, MS, CBSP, M(ASCP), BSM(ASM), CM(ACM),
Hazards Control Department,
Lawrence Livermore National Laboratory,
7000 East Avenue MS-289, Livermore, CA 94550,
Phone:925 423-7385, Fax:423-1086,
Jin2@
>Elizabeth,
>
>It is certainly possible that attenuated bacterial strains could
>take up "promiscuous" plasmids from the environment through
>inadvertant transformation, which might restore pathogenicity or
>drug resistance.
>
>Under natural conditions, plasmids are transmitted to new bacterial
>hosts by a process known as bacterial conjugation. However, the
>plasmid vectors in common use for molecular cloning (mostly based on
>pMB1 which was originally isolated from a clinical specimen) methods
>lack a copy of the gene that is required for conjucation, and are
>thus incapable of spontaneously transforming new hosts. However,
>some older cloning vectors based on pBR322 may be co-mobilized with
>an environmental plasmid. If such a vector were to carry a drug
>resistance gene, for instance, this phenotype could be confered upon
>an environmental host quite unintentionally with potentially
>hazardous results.
>
>As someone who works with these organisms as a researcher, and not
>strictly speaking a biosafety professional, I can assure you that the
>risk may be small, but it definitely exists.
>
>James
>
>
>--
>This concerns the utilization of non-pathogenic bacteria
>- a particular strain of an organism is missing part of
>its structure which is required to cause disease.
>(Sorry, I don't know exactly what is missing...)
>
>In discussing required safety measures with a fellow
>employee, a claim was made by the individual that a
>particular safety practice (that is more stringent than
>I thought necessary) had been originally employed
>because it was claimed/concerned the organisms would
>suddenly "revert to their wild state and become
>pathogenic again".
>
>My question is: given microorganisms with no
>interaction with the outside world, can bacteria just
>"switch" from one strain to another - when it would
>require genesis of cellular structures that don't
>currently exist?
>
>As I understand, the specific bacteria in question are
>missing a plasmid(?) which is necessary for
>pathogenesis. If it isn't there, how's a cell going to
>just suddenly decide "hey, I'll produce something for
>which I have no DNA to build?"
>
>If there is a short answer, I would appreciate it.
>
>If there is a long answer, a reference to a good source
>for the answer would also be appreciated.
>
>And, if it's pretty boring and wouldn't interest any one
>else, please feel free to respond to me directly at:
>safety_queen@
>
>Thank you in advance!
>
>
>Elizabeth E. Smith
>Environmental, Health & Safety Manager
>BioPort Corporation
>Lansing, Michigan
>517-327-6806
=========================================================================
Date: Tue, 23 Nov 1999 07:37:14 -1000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Thomas Goob
Subject: Stool Analysis for Parasites: mixed waste stream
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Dear list members:
We are generating a mixed waste stream as a result of the subject
diagnostic test (stool analysis for parasites). Specifically, the waste
mixture consists of polyvinyl alcohol, glycerin, ethyl alcohol, isopropyl
alcohol, methyl alcohol and mercuric chloride (at 4%), mixed with stool.
As this waste stream has hazardous chemicals, my biological waste
contractor will not touch it.
My hazardous chemical waste contractor said he could dispose of the waste,
provided a plate count could be provided to document that the waste is
biological inactive. We did this and was happy to find no growth.
However, I am now informed that because the waste mixture has greater than
260 ppm mercury compounds, that the mercury must be recovered. Therefore,
this waste is costing about 10 times more to dispose of compared to typical
hazardous chemical waste.
We have an alternative method we can follow which does not contain any
mercury. The alternative method creates a mixture of stool with glycerol,
glacial acetic acid, zinc sulfate, polyvinyl alcohol, ethyl alcohol, methyl
alcohol, and isopropyl alcohol. However, our plate counts reveal some
minimal biological activity with this alternative method, so my chemical
waste contractor wont touch it.
Does anyone else do this test and generate this type of waste stream? How
are you disposing of it? We are generating about 25 15ml viles of this
mixture everyday!! Any advise, help or shared experiences would be great.
Thanks,
Tom Goob
Diagnostic Laboratory Services, Inc.
tgoob@dls.
=========================================================================
Date: Tue, 23 Nov 1999 10:18:32 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: Ethidium Bromide Gels
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
At UCSF, we treat both the acrylamide and the ethidium bromide as hazardous
and ask labs to collect used gels and associated reagents in wide-mouth
disposable plastic jars with a Haz Chem label. We pick these jars up and
dispose of them as chemical waste.
------------------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
University of California, San Francisco
Voice 415-476-2097
Fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Jean.Goldberg [mailto:Jean.Goldberg@MED.NYU.EDU]
Sent: Tuesday, November 23, 1999 8:37 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Ethidium Bromide Gels
We are trying to decide whether or not to treat gels that
have ethidium bromide in them as hazardous waste. I would
appreciate hearing from others about their rationale for
making a decision on this issue. Thanks in advance.
----------------------------------------
Jean Goldberg
Email: Jean.Goldberg@Med.Nyu.Edu
"NYU Medical Center"
=========================================================================
Date: Tue, 23 Nov 1999 15:03:38 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stefan Wagener
Subject: Re: Stool Analysis for Parasites: mixed waste stream
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
What do you mean by minimal biological activity, growth of organisms? What
is the control you are using? If you still have some space in your vials,
can you add some more of the acid or alcohols and see if you still get
growth. If that doesn't work at some phenol to it. No Bleach or aldehydes
because of compatibility issues.
Test it and see if you can kill the bugs.
Hope this helps.
Stefan :-)
Stefan Wagener, Ph.D, CBSP
Michigan State University, ORCBS
C-126 Research Complex Engineering
East Lansing, MI 48824
Phone: (517) 355-6503
Fax: (517) 353-4871
We have an alternative method we can follow which does not contain any
mercury. The alternative method creates a mixture of stool with glycerol,
glacial acetic acid, zinc sulfate, polyvinyl alcohol, ethyl alcohol, methyl
alcohol, and isopropyl alcohol. However, our plate counts reveal some
minimal biological activity with this alternative method, so my chemical
waste contractor wont touch it.
=========================================================================
Date: Tue, 23 Nov 1999 15:08:11 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "J.H. Keene"
Subject: Re: Stool Analysis for Parasites: mixed waste stream
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
If you use the alternative technique (without the mercuric chloride) why
can't you just flush this down the drain? 375 ml/day is not a great amount
of material and this would be going to the waste water treatment plant that
is designed to handle this particular type of biological waste. I would
recommend checking with the sewage treatment people and determine if they
would allow this to go into the sanitary sewer.
----- Original Message -----
From: Thomas Goob
To:
Sent: Tuesday, November 23, 1999 12:37 PM
Subject: Stool Analysis for Parasites: mixed waste stream
> Dear list members:
>
> We are generating a mixed waste stream as a result of the subject
> diagnostic test (stool analysis for parasites). Specifically, the waste
> mixture consists of polyvinyl alcohol, glycerin, ethyl alcohol, isopropyl
> alcohol, methyl alcohol and mercuric chloride (at 4%), mixed with stool.
> As this waste stream has hazardous chemicals, my biological waste
> contractor will not touch it.
>
> My hazardous chemical waste contractor said he could dispose of the waste,
> provided a plate count could be provided to document that the waste is
> biological inactive. We did this and was happy to find no growth.
> However, I am now informed that because the waste mixture has greater than
> 260 ppm mercury compounds, that the mercury must be recovered. Therefore,
> this waste is costing about 10 times more to dispose of compared to
typical
> hazardous chemical waste.
>
> We have an alternative method we can follow which does not contain any
> mercury. The alternative method creates a mixture of stool with glycerol,
> glacial acetic acid, zinc sulfate, polyvinyl alcohol, ethyl alcohol,
methyl
> alcohol, and isopropyl alcohol. However, our plate counts reveal some
> minimal biological activity with this alternative method, so my chemical
> waste contractor wont touch it.
>
> Does anyone else do this test and generate this type of waste stream? How
> are you disposing of it? We are generating about 25 15ml viles of this
> mixture everyday!! Any advise, help or shared experiences would be great.
> Thanks,
>
>
>
> Tom Goob
> Diagnostic Laboratory Services, Inc.
> tgoob@dls.
>
=========================================================================
Date: Wed, 24 Nov 1999 08:30:27 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Loreene Broker
Subject: Job Description
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
I am trying to gather some information that may be used to write a
job description for an institutional BSO. Currently the functions
of a BSO are rather fragmented across campus and we might be moving
in the direction of establishing a single BSO in the very near
future.
If possible, I would appreciate a copy of your BSO's job
description. Please feel free to send it via FAX or email. I will
remove all identifying information (personal or institutional)
before sharing the information on my campus.
Thank you for your help.
WMU WMU WMU WMU WMU WMU WMU WMU WMU WMU
Loreene L. Broker
Research Compliance Coordinator
Western Michigan University
327E Walwood Hall
Kalamazoo, MI 49008-5162
Phone: 616 387 8293
FAX: 616 387 8276
email: loreene.broker@wmich.edu
URL:
WMU WMU WMU WMU WMU WMU WMU WMU WMU WMU
=========================================================================
Date: Wed, 24 Nov 1999 09:39:24 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Francis Churchill
Subject: Re: Stool Analysis for Parasites: mixed waste stream
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
>We have an alternative method we can follow which does not contain any
>mercury. The alternative method creates a mixture of stool with glycerol,
>glacial acetic acid, zinc sulfate, polyvinyl alcohol, ethyl alcohol, methyl
>alcohol, and isopropyl alcohol. However, our plate counts reveal some
>minimal biological activity with this alternative method, so my chemical
>waste contractor wont touch it.
You could have an envoironmental testing lab check the samples for
flammability (alcohols), pH (acetic acid) and zinc (if zinc is regulated by
your state). If these are all negative, your biowaste contractor should be
able to handle it, or you can flush it if this doesn't increase our
potential for exposure.
If the alcohols or other levels are high enough to make this an EPA
hazardous waste, then it needs to go with the chemical waste contractor.
You may just have to add a disinfectant to kill everything. Stephan
covered that well, more alcohols or acetic acid are the best bet. Phenol
increases the potential chemical exposure to the lab tech.
Mercury compounds are expensive to dispose, especially mercuric chloride.
The Cl- eats up the mercury retorter. Avoid this route if it is possible -
and it sounds as though it is.
Francis
Alcohol and calculus don't mix. Never drink and derive.
Francis Churchill, IHIT
University of Vermont - Environmental Safety Facility
655D Spear Street, Burlington, VT 05405-3010
(802) 656-5405
fchurchi@zoo.uvm.edu
=========================================================================
Date: Wed, 24 Nov 1999 09:02:29 -1000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Hubert B Olipares
Subject: Re: DOT/IATA other Shipping and Training requirements
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
We have Saft-Pak do our University's training and as stated from them:
IATA has a 2 year recurrency requirement, also the IATA paperwork and
documentation is different than 49 CFR, You are however trained as far as
49CFR is concerned if you are IATA trained. There are differences between
IATA and DOT but, IATA is more stringent and if you follow IATA basically
you are covered under DOT.
More information of saft-pak and they
have a CD-ROM training found at
TRAINING 172.700
- General - HazMat Employers and Employees. 173.1
- Training for Packaging. 174.7
- By Rail. 175.20
- By Air. 176.13
- By Vessel. 177.800
- By Road.
177.816
- Driver Training
==============================================================================
Hubert B. Olipares, RBP
Biological Safety Officer
University of Hawaii
Environmental Health and Safety Office
2040 East-West Road
Honolulu, Hawaii 96822-2022
Telephone: 808-956-3197
Fax: 808-956-3205
E-Mail: olipares@hawaii.edu
=========================================================================
Date: Wed, 24 Nov 1999 15:13:17 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Anderson, Bruce"
MIME-Version: 1.0
Content-Type: text/plain; charset="ISO-8859-1"
I have been asked by a researcher if acetone fixation will kill coxsackie B4 virus. They have a new procedure which requires fixation of the slides for 10 minutes in ice-cold 100% acetone, followed by incubation of the antibody. They were wondering if
this will neutralize the virus?
I'm hoping someone out there may have some references on this.
Thanks in advance. Bruce
> T. Bruce Anderson
> Biosafety Officer
> The University of British Columbia
> 50- 2075 Wesbrook Mall
> Vancouver, BC Canada
> * anderson@safety.ubc.ca
> *
> * (604) 822-7596
> * (604) 880-0711 cellular
>
=========================================================================
Date: Mon, 29 Nov 1999 08:01:58 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Susan Kingston
Subject: second try - immunocompromised animals and human tumor cell lines
Mime-Version: 1.0
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boundary="=====================_996282==_.ALT"
--=====================_996282==_.ALT
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Hello Biosafety Folks,
I had sent a message to the listserver a week ago asking for help/information
regarding the use of human tumor cell lines in immunocompromised animals. I'm
hoping that it was the holiday that kept people from responding and not the
fact that the issue is too sticky! Any information/references/opinions to the
following questions would be greatly appreciated!
What Biosafety Level do you assign to nude mice or other immunocompromised
animals that are inoculated with human tumor cell lines?
How do you address the issue of silent infections in these animals with an
agent
that is not causing cytopathologic effects in the cell line? Specifically, how
do you address the aerosolization issue of Lymphocytic Choriomeningitis Virus?
Do you require any special testing of the cell line or the animals? If you do,
which agents are you specifically looking for?
Thanks to all!
Susan Kingston
--------------------------------------------
Susan K. Kingston DVM, MS
Biological Safety Professional
University of Illinois
102 Environmental Health and Safety Building
101 S. Gregory Street
Urbana, IL 61801-3070
(217)244-1939, fax (217)244-6594
email: skingsto@uiuc.edu
--------------------------------------------
--=====================_996282==_.ALT
Content-Type: text/html; charset="us-ascii"
Hello Biosafety Folks,
I had sent a message to the listserver a week ago asking for
help/information regarding the use of human tumor cell lines in
immunocompromised animals. I'm hoping that it was the holiday that
kept people from responding and not the fact that the issue is too
sticky! Any information/references/opinions to the following
questions would be greatly appreciated!
What Biosafety Level do you assign to nude mice or other
immunocompromised animals that are inoculated with human tumor cell
lines?
How do you address the issue of silent infections in these animals
with an agent that is not causing cytopathologic effects in the
cell line? Specifically, how do you address the aerosolization
issue of Lymphocytic Choriomeningitis Virus?
Do you require any special testing of the cell line or the animals?
If you do, which agents are you specifically looking
for?
Thanks to all!
Susan Kingston
--------------------------------------------
Susan K. Kingston DVM, MS
Biological Safety Professional
University of Illinois
102 Environmental Health and Safety Building
101 S. Gregory Street
Urbana, IL 61801-3070
(217)244-1939, fax (217)244-6594
email: skingsto@uiuc.edu
--------------------------------------------
--=====================_996282==_.ALT--
=========================================================================
Date: Mon, 29 Nov 1999 08:05:52 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: second try - immunocompromised animals and human tumor cell l
ines
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Hi, Susan -
At UCSF, we require all cell cultures of human origin (including
well-established cell lines) to be handled under at least BSL2 containment.
If we have reason to believe the cell lines may harbor agents of special
concern (such as the BCBL1 cell line as a source of HHV8), we may require
additional containment. Nude mice and other immuncompromised animals are
handled by us only under barrier conditions and under no circumstances are
mice infected or even potentially infected with LCM or any other rodent
agent permitted in the barrier facility. All incoming animals undergo 4-6
weeks of quarantine and testing. Animals purposely infected with an agent
under circumstances that make aerosolization possible are always handled in
a biosafety cabinet.
Hope this helps.
-- Glenn
------------------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
University of California, San Francisco
Voice 415-476-2097
Fax 415-476-0581
glennf@ehsmail.ucsf.edu
-----Original Message-----
From: Susan Kingston [mailto:skingsto@UIUC.EDU]
Sent: Monday, November 29, 1999 6:02 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: second try - immunocompromised animals and human tumor cell lines
Hello Biosafety Folks,
I had sent a message to the listserver a week ago asking for
help/information regarding the use of human tumor cell lines in
immunocompromised animals. I'm hoping that it was the holiday that kept
people from responding and not the fact that the issue is too sticky! Any
information/references/opinions to the following questions would be greatly
appreciated!
What Biosafety Level do you assign to nude mice or other immunocompromised
animals that are inoculated with human tumor cell lines?
How do you address the issue of silent infections in these animals with an
agent that is not causing cytopathologic effects in the cell line?
Specifically, how do you address the aerosolization issue of Lymphocytic
Choriomeningitis Virus?
Do you require any special testing of the cell line or the animals? If you
do, which agents are you specifically looking for?
Thanks to all!
Susan Kingston
--------------------------------------------
Susan K. Kingston DVM, MS
Biological Safety Professional
University of Illinois
102 Environmental Health and Safety Building
101 S. Gregory Street
Urbana, IL 61801-3070
(217)244-1939, fax (217)244-6594
email: skingsto@uiuc.edu
--------------------------------------------
=========================================================================
Date: Mon, 29 Nov 1999 11:17:56 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
Comments: RFC822 error: MESSAGE-ID field duplicated. Last occurrence
was retained.
From: Irene Cooke
Subject: Biosafety Professional Position Advertisement
Mime-Version: 1.0
Content-Type: multipart/alternative;
boundary="=====================_13688162==_.ALT"
--=====================_13688162==_.ALT
Content-Type: text/plain; charset="us-ascii"
Biological Safety Professional
University of Illinois at Urbana-Champaign
The Division of Environmental Health and Safety is seeking an individual to
assist in a program that includes all aspects of biological safety with
particular emphasis on research activities involving recombinant DNA molecules
and biohazardous agents. This is a full-time academic professional position
starting as soon as possible after the closing date. The individual will work
under the direction of the Assistant Director, Environmental Health and Safety,
Biological Safety Section. An advanced degree in microbiology, cellular
biology, biochemistry, genetics, botany, molecular biology, or related field
strongly preferred. Minimum requirement of a B.S. in any one of the above
areas with relevant professional experience in the field of biosafety
required. Special consideration will be given to candidates with experience in
plant systems and recombinant DNA techniques. Ability to communicate
effectively orally and in writing, and to work as a member of a health and
safety team is required. The position requires the use of computer word
processing and database applications. To ensure full consideration, candidates
should submit a letter of application, a resume, a statement of professional
qualifications, and three letters of reference by January 28, 2000. Send
application materials to: Irene Cooke, Assistant Director, Division of
Environmental Health and Safety, 102 Environmental Health and Safety Building,
101 South Gregory Street, Urbana, IL 61801. For additional information, call
217/244-7801 or email i-cooke@uiuc.edu. Salary is commensurate with
qualifications and experience.
The University of Illinois at Urbana-Champaign is an Affirmative Action/Equal
Opportunity Employer.
Irene Cooke, D.V.M., Ph.D.
Head, Biological Safety Section
Division of Environmental Health and Safety
University of Illinois at Urbana-Champaign
Tel #: (217) 244-7801
Fax #: (217) 244-6594
--=====================_13688162==_.ALT
Content-Type: text/html; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
Biological Safety Professional
University of Illinois at Urbana-Champaign
The Division of Environmental Health and Safety is seeking an individual
to assist in a program that includes all aspects of biological safety
with particular emphasis on research activities involving recombinant DNA
molecules and biohazardous agents. This is a full-time academic
professional position starting as soon as possible after the closing
date. The individual will work under the direction of the Assistant
Director, Environmental Health and Safety, Biological Safety
Section. An advanced degree in microbiology, cellular biology,
biochemistry, genetics, botany, molecular biology, or related field
strongly preferred. Minimum requirement of a B.S. in any one of the
above areas with relevant professional experience in the field of
biosafety required. Special consideration will be given to
candidates with experience in plant systems and recombinant DNA
techniques. Ability to communicate effectively orally and in
writing, and to work as a member of a health and safety team is
required. The position requires the use of computer word processing
and database applications. To ensure full consideration, candidates
should submit a letter of application, a resume, a statement of
professional qualifications, and three letters of reference by January
28, 2000. Send application materials to: Irene Cooke, Assistant
Director, Division of Environmental Health and Safety, 102 Environmental
Health and Safety Building, 101 South Gregory Street, Urbana, IL
61801. For additional information, call 217/244-7801 or email
i-cooke@uiuc.edu. Salary is commensurate with qualifications and
experience.
The University of Illinois at Urbana-Champaign is an Affirmative
Action/Equal Opportunity Employer.
Irene Cooke, D.V.M., Ph.D.
Head, Biological Safety Section
Division of Environmental Health and Safety
University of Illinois at Urbana-Champaign
Tel #: (217) 244-7801
Fax #: (217) 244-6594=20
--=====================_13688162==_.ALT--
=========================================================================
Date: Mon, 29 Nov 1999 14:51:57 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
At 03:13 PM 11/24/99 -0800, T. Bruce Anderson wrote:
>I have been asked by a researcher if acetone fixation will kill coxsackie B4
vir
>us.
I could find very little info regarding acetone and germicidal action. There
is a brief note in the 3rd edition of Disinfection, Sterilization, and
Preservation that acetone is less effective as a skin antiseptic then
alcohol.
Isopropanol is ineffective against coxsackie virus (according to the 4th
edition), ethanol at >60% is effective when there is a contact time of 10
minutes.
In general hydrophilic viruses are harder to inactivate then lipophilic and
cold temperatures decreases the activity of germicides. Since acetone is less
active then alcohol and ethanol is only slowly cidal at room temperature, I
would not be comfortable that ice cold acetone is doing any killing.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Mon, 29 Nov 1999 15:24:36 -0400
Reply-To: Harriet Izenberg
Sender: A Biosafety Discussion List
From: Harriet Izenberg
Subject: Biosafety Position
MIME-Version: 1.0
Content-Type: text/plain; charset="US-Ascii"
Content-Transfer-Encoding: quoted-printable
Subject: Time: 3:16
PM
Biosafety Position =20
11/29/99
Date:
Biosafety Officer
The University of Pennsylvania, Office of Environmental Health and
Radiation Safety, is seeking a qualified candidate to work under the gene=
ral
direction of the Institutional Biosafety Officer. The individual is
responsible to:
o Assist in the development and implementation of the University=92s
Biological Safety Program.
o Conduct laboratory safety audits, make recommendations and write
reports.
o Conduct research grant protocol review.
o Respond (on call) to incidents and emergencies involving biohazards.
o Support biological safety training and educational efforts.
o Participate in interpreting federal and local regulations and
guidelines; monitor and assist in assuring compliance with regulations.
o Provide support to "U of P HAZMAT Team."
Qualifications
Bachelor=92s Degree in Science required; M.S. preferred; 3-5 years
experience in biological safety, preferably at an academic institution. =
Strong
background in medical microbiology/molecular biology is essential.
Must possess excellent verbal and written communication skills; must be
computer literate.
Professional biosafety certification or eligibility desirable.
Must be able to move about freely and carry up to 30 pounds; must be
capable of utilizing respiratory protection, including SCBA=92s. Valid dr=
ivers
license.
Send a cover letter, explaining your interest and capabilities, along
with a resume or CV to:=20
Ms. Christine Belden, Business Manager
Office of Environmental Health and Radiation Safety
University of Pennsylvania
14th floor Blockley Hall
Philadelphia, Pa 19104-6021
=========================================================================
Date: Tue, 30 Nov 1999 09:41:22 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Dan Shawler
Subject: Experimental Chemotherapy in Animals
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
We have investigators who perform chemotherapy experiments in rats and mice.
According to the literature a large portion of the injected drug is secreted
in the urine intact. Most of the remaining drug is metabolized to equally
hazardous molecules and is also secreted in the urine. Calculations show
that if 100% of the injected drug is secreted by all the animals in the
cage, the bedding material will contain < 0.5% of the standard therapeutic
dose of the drug.
My question is, what is the proper disposal method for the soiled bedding?
I have inquired with our local county Health Department, who have
enforcement jurisdiction for hazardous and biohazardous materials. Despite
the presence of a large biotech community in our location, the Health
Department officials did not have an answer and more or less left it up to
the individual company or insitution to decide.
What do your institutions do in this situation?
Thanks for your help.
Dan Shawler
Safety Officer
Sidney Kimmel Cancer Center
=========================================================================
Date: Wed, 1 Dec 1999 12:30:16 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Wan Yu Kwan
Subject: Re: Experimental Chemotherapy in Animals
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Dan
We got a similar problem. We feed the rats with food that implemented with
carcinogen. The bedding of wood chips will generate the dust that will be
inhaled by the staff. Thus, the first solution on changing the bedding
material is to use non-dust material e.g. the absorbent pad.
The experiment has not been started as the Principal Investigator need to
prove the protocols is safe to staff and environment.
On the chemical wastes, will the metabolites be destroyed by any simple
chemical treatment to lower the hazards and sterilize the used materials?
The autoclave may generate the vapor of the drugs or metabolites that are
not easily controlled.
YK Wan
Safety Officer, CUHK
At 09:41 AM 11/30/99 -0800, you wrote:
>We have investigators who perform chemotherapy experiments in rats and mice.
>According to the literature a large portion of the injected drug is secreted
>in the urine intact. Most of the remaining drug is metabolized to equally
>hazardous molecules and is also secreted in the urine. Calculations show
>that if 100% of the injected drug is secreted by all the animals in the
>cage, the bedding material will contain < 0.5% of the standard therapeutic
>dose of the drug.
>
>My question is, what is the proper disposal method for the soiled bedding?
>I have inquired with our local county Health Department, who have
>enforcement jurisdiction for hazardous and biohazardous materials. Despite
>the presence of a large biotech community in our location, the Health
>Department officials did not have an answer and more or less left it up to
>the individual company or insitution to decide.
>
>What do your institutions do in this situation?
>
>Thanks for your help.
>
>Dan Shawler
>Safety Officer
>Sidney Kimmel Cancer Center
>
>
Please reply when you receive the message. Thank you.
***** Yu Kwan WAN,
***** Safety Officer
***** The Chinese University of Hong Kong
***** Shatin, NT, Hong Kong
***** Email:
***** ulsoykwan@cuhk.edu.hk
***** ulsoykwan@
***** ulsoykwan@
***** ulsoykwan@
***** ulsoykwan@
=========================================================================
Date: Thu, 2 Dec 1999 10:20:55 +0000
Reply-To: Thomas Boyle
Sender: A Biosafety Discussion List
From: Thomas Boyle
Subject: Handling Brain Tissue
MIME-Version: 1.0
Content-Transfer-Encoding: 7bit
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I am posting the following for a friend. Thank you in advance for your
responses.
-----------
I'm trying to find out what other groups require when handling brain
tissue. The risk of CJD from a random brain sample is estimated at
1:1,000,000. Do people add any precautions above and beyond the BBP regulations
(higher concentration of bleach, use of NaOH, all inexpensive materials
disposed of, etc.) or is the perceived risk too low for additional precautions?
No samples are presumed to have or proven to have CJD, so the
precautions specified in the BMBL are not strictly called for.
____________________________________
Thomas Boyle
Biological Safety Officer
Environmental Health and Radiation Safety
University of Pennsylvania
14th Floor Blockley Hall
Philadelphia, Pa. 19104-6021
215.898.3569 (phone)
215.898.0140 (FAX)
tom@ehrs.upenn.edu
=========================================================================
Date: Thu, 2 Dec 1999 10:45:34 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Jean.Goldberg"
Subject: Rabbits and Replication Deficient Adenovirus
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; CHARSET=US-ASCII
Dear Group, Many thanks for your help in the past. I have
a new dilemma, with which I need some assistance. I have a
researcher would like to inject the joints of rabbits with
a replication deficient adenovirus carrying the gene for
interleukin 1 type 2 receptor. We are trying to determine
if we can accommodate his research. We have a workroom with
a biosafety cabinet where he can inject the virus.
However, it would be difficult for us to dedicate a rabbit
room to Animal Biosafety Level 2 after the injections. The
rabbits would have to go back into a standard animal room
with other rabbits. Please give me your thoughts on this.
Also, if you think the rabbits should be housed apart from
other rabbits -- could you let me know the time period for
this separation (e.g. 2 days, 1 week . .) As always, thanks
in advance for your assistance. -- Jean
----------------------------------------
Jean Goldberg
Email: Jean.Goldberg@Med.Nyu.Edu
"NYU Medical Center"
=========================================================================
Date: Thu, 2 Dec 1999 12:06:28 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Elizabeth Smith
Subject: dye job for lab animals
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
I have an employee who uses dyes on animals for a procedure.
They are currently using picric acid as one of the colors. Due to the cost
of disposing of old picric acid and the potentical chemical hazards of the
compound, they want a new dye material.
The parameters need to be:
yellow or orange color
last about 2 weeks on guinea pig fur
very limited toxicity or non-toxic to guinea pigs by topical exposure (it is
crucial that the material doesn't effect the health of the guinea pigs just
by having their fur stained).
Can someone recommend a compound? Please reply directly to me at
safety_queen@. Thank you for any assistance rendered.
Elizabeth E. Smith
Environmental, Health & Safety Manager
BioPort Corporation
Lansing, Michigan
517-327-6806
There is nothing to learn from history but that men do not learn from
history. - Hegel
__________________________________________________
Do You Yahoo!?
Thousands of Stores. Millions of Products. All in one place.
Yahoo! Shopping:
=========================================================================
Date: Thu, 2 Dec 1999 15:21:15 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Susan Souder
Subject: Floor drains
MIME-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: 7bit
Hi,
I have a question regarding floor drains in a ABSL-3 suite. The BMBL
requires that floor drains always be filled with a disinfectant. Would
anyone care to give me some suggestions as to what disinfectants you are
using? Also, if you have floor drains in a large animal ABSL-2,
containing pigs, sheep, etc. would a disinfectant be needed there also?
I am in need of some feedback!
Thank you,
Susan Souder, M.S.
Biological Safety Officer
Thomas Jefferson University
Phila., Pa., 19107
Phone: 215-503-7422
Fax: 215-503-7727
=========================================================================
Date: Thu, 2 Dec 1999 12:33:43 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Wolz, Sarah"
Subject: Mushroom farm decon
MIME-Version: 1.0
Content-Type: text/plain
Somewhat related to biosafety....
I was asked recently how one would go about decontaminating a barn (!) that
is used to propagate food-grade mushrooms. Apparently the mushroom farmers
have a contamination problem with the wrong type of fungal spore invading
their mushroom cultures. They want to empty the barn, fog with something,
and then reintroduce their cultures under more isolated conditions. What
would you all recommend as a decon agent to "fog" a mushroom growing barn??
My last mycology class was longer ago that I can believe...
Thanks!
Sarah Wolz
Pathogenesis Corp.
swolz@
=========================================================================
Date: Thu, 2 Dec 1999 16:14:09 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Paul Jennette
Subject: Condensate from BSL-3 Autoclaves
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We are concerned about condensate from the initial stages of autoclave cycles
being unsterile and potentially contaminated with infectious agents. The
condensate drain from a large autoclave at our new BSL-3 facility is located on
the "clean" side of the autoclave and discharges (by plumbing code) to an open
drain.
We would like to know how condensate from autoclaves in BSL-3 laboratories is
managed at other facilities and would appreciate your responses to the
following:
1. Where (clean side or dirty side) are the condensate drains from your BSL-3
autoclaves?
2. Do you route the discharges to a kill tank?
3. Do you do anything to control the release of infectious agents from the
drains?
Thanks very much in advance for your help!
J. Paul Jennette, P.E.
Biosafety Engineer
Cornell University
College of Veterinary Medicine
Biosafety Program
S3-010 Schurman Hall, Box 38 (607) 253-4227
Ithaca, New York 14853-6401 fax -3723
--=====================_27254742==_.ALT
Content-Type: text/html; charset="us-ascii"
We are concerned about condensate from the initial stages of
autoclave cycles being unsterile and potentially contaminated with
infectious agents. The condensate drain from a large autoclave at
our new BSL-3 facility is located on the "clean" side of the
autoclave and discharges (by plumbing code) to an open drain.
We would like to know how condensate from autoclaves in BSL-3
laboratories is managed at other facilities and would appreciate your
responses to the following:
1. Where (clean side or dirty side) are the condensate drains
from your BSL-3 autoclaves?
2. Do you route the discharges to a kill tank?
3. Do you do anything to control the release of infectious
agents from the drains?
Thanks very much in advance for your help!
J. Paul Jennette, P.E.
Biosafety Engineer
Cornell University
College of Veterinary Medicine
Biosafety Program
S3-010 Schurman Hall, Box
38 (607)
253-4227
Ithaca, New York
14853-6401 fax
-3723
--=====================_27254742==_.ALT--
=========================================================================
Date: Thu, 2 Dec 1999 15:54:45 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Betty Kupskay
Subject: Re: Condensate from BSL-3 Autoclaves
Mime-Version: 1.0
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In our BSL-3 labs for human pathogens, the autoclave drains have a solid
connection (with mixing box and HEPA-filtered vent) and are on the 'dirty' side,
i.e. in containment. These drains are piped to the sanitary sewer, as per the
reg's from the Health Canada Office of Biosafety (Laboratory Safety Guidelines,
1996).
On our animal pathogen side, the drains from all autoclaves in BSL-3 also have a
solid connection (with mixing box and HEPA-filtered vent) and are on the 'dirty'
side, but are piped directly to our liquid effluent cooker system. This is a
requirement for animal pathogens as set out in Containment Standards for
Veterinary Facilitiesby Agriculture and Agri-Food Canada, 1996.
Hope this helps.
Betty Kupskay
Biosafety Specialist/Health Canada
Canadian Science Centre for Human and Animal Health
1015 Arlington St., Suite A1010
Winnipeg, MB R3E 3P6
Ph: 204-789-2065
Fax: 204-789-2069
EMail: betty_kupskay@hc-sc.gc.ca
Paul Jennette on 99/12/02 03:14:09 PM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Betty Kupskay)
Subject: Condensate from BSL-3 Autoclaves
We are concerned about condensate from the initial stages of autoclave cycles
being unsterile and potentially contaminated with infectious agents. The
condensate drain from a large autoclave at our new BSL-3 facility is located on
the "clean" side of the autoclave and discharges (by plumbing code) to an open
drain.
We would like to know how condensate from autoclaves in BSL-3 laboratories is
managed at other facilities and would appreciate your responses to the
following:
1. Where (clean side or dirty side) are the condensate drains from your BSL-3
autoclaves?
2. Do you route the discharges to a kill tank?
3. Do you do anything to control the release of infectious agents from the
drains?
Thanks very much in advance for your help!
J. Paul Jennette, P.E.
Biosafety Engineer
Cornell University
College of Veterinary Medicine
Biosafety Program
S3-010 Schurman Hall, Box 38 (607) 253-4227
Ithaca, New York 14853-6401 fax -3723
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Date: Thu, 2 Dec 1999 15:25:10 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Edwin Jackson
Subject: Re: Mushroom farm decon
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Peracetic acid (also called peroxyacetic acid) is available for
fogging in agricultural settings. Peroxyacetic acid is listed as a
cold sterilant by the EPA so it has a broad activity spectrum. It
decomposes to H2O and dilute acetic acid.
Address: 122 TOMH BYU
Provo, UT 84602-0100
Telephone: (801) 378-5779
Fax (801 378-5977
e-mail edwin_jackson@byu.edu
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Date: Thu, 2 Dec 1999 16:26:45 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Ben J. Elder"
Subject: Re: Floor drains
MIME-Version: 1.0
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Susan,
I would contact Mr. Sherman McDonald, VP of the Food Industries Div., at
Rochester Midland Corp.
They manufacture an iodine block to be tossed into the drain without
removing the wrapping which also dissolve. The iodine in the block will
kill anything in the drail as it dissolves over a period of weeks. He can
also send you a drain cleaning procedure that will be very helpful as it was
to us.
His e-mail address is shermanmcdonald@.
Ben Elder
----- Original Message -----
From: Susan Souder
To:
Sent: Thursday, December 02, 1999 2:21 PM
Subject: Floor drains
> Hi,
> I have a question regarding floor drains in a ABSL-3 suite. The BMBL
> requires that floor drains always be filled with a disinfectant. Would
> anyone care to give me some suggestions as to what disinfectants you are
> using? Also, if you have floor drains in a large animal ABSL-2,
> containing pigs, sheep, etc. would a disinfectant be needed there also?
> I am in need of some feedback!
> Thank you,
> Susan Souder, M.S.
> Biological Safety Officer
> Thomas Jefferson University
> Phila., Pa., 19107
> Phone: 215-503-7422
> Fax: 215-503-7727
=========================================================================
Date: Fri, 3 Dec 1999 08:50:17 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: Rabbits and Replication Deficient Adenovirus
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Hi Jean,
I can sympathize with you dilemma. In universities, funding is short, and
research is moving in directions that call out for more (and expensive)
controls. I don't have perfect answers but here's my thoughts:
There are several points to consider doing a risk assessment with
adenovirus.
First, be aware that the production of replication deficient adenovirus,
which is usually deleted in the E1 region, may create a background of
wildtype virus due to contamination with undigested viral DNA or religation
of the wild-type fragments. These wildtypes can become amplified with serial
passage of the adeno stocks. For this reason we always treat adenovirus as
BSL2.
You can also ask the researcher for evidence, through assay, that wild type
virus is not present. and ask that they not serially pass the stocks.
Second, is interleukin 1 type 2 receptor a human gene? If so, that's
another reason for maintaining BSL2 conditions. You never want to risk
overexpression of a human gene in the animal workers or lab workers and
adenovirus is a very effective vector.
Third, what about the other rabbits and the investigator's work with them?
Not seeing the whole protocol, I couldn't really assess the risk, but it's
likely the other researcher's won't want adeno infected rabbits in the same
animal room.
Instead of speculating on whether and how long the rabbits may shed virus,
ask the investigator for assay results. He/she may need to do some
preliminary work or at least provide sufficient references to show that the
rabbits will pose no hazards if not maintained at BSL2.
You may be able to accommodate the investigator with a separate rabbit room
and respiratory protection for the animals workers and investigators and
with assays at each step that show the wild type virus isn't present.
Good luck, feel free to contact me if you need references on this stuff.
I'd be interested on how you eventually resolve this.
Janice Flesher, MS, CBSP
Principle Industrial Hygienist/Biosafety Officer
EOHSS - University Medical Dental School of NJ
97 Paterson St. #227
New Brunswick, NJ, 08901
(732) 235-8497 phone
(732) 235-8499 fax
fleshejk@umdnj.edu
-----Original Message-----
From: Jean.Goldberg
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Thursday, December 02, 1999 11:10 AM
Subject: Rabbits and Replication Deficient Adenovirus
>Dear Group, Many thanks for your help in the past. I have
>a new dilemma, with which I need some assistance. I have a
>researcher would like to inject the joints of rabbits with
>a replication deficient adenovirus carrying the gene for
>interleukin 1 type 2 receptor. We are trying to determine
>if we can accommodate his research. We have a workroom with
>a biosafety cabinet where he can inject the virus.
>However, it would be difficult for us to dedicate a rabbit
>room to Animal Biosafety Level 2 after the injections. The
>rabbits would have to go back into a standard animal room
>with other rabbits. Please give me your thoughts on this.
>Also, if you think the rabbits should be housed apart from
>other rabbits -- could you let me know the time period for
>this separation (e.g. 2 days, 1 week . .) As always, thanks
>in advance for your assistance. -- Jean
>
>----------------------------------------
>Jean Goldberg
>Email: Jean.Goldberg@Med.Nyu.Edu
>"NYU Medical Center"
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=========================================================================
Date: Sat, 4 Dec 1999 17:04:27 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: James Scott
Organization: University of Toronto Botany
Subject: Re: Mushroom farm decon
MIME-Version: 1.0
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Sarah,
From a mycological perspective, your question is nearly impossible.
Practically any kind of chemical foggin that I can think of will not
be sufficient to prevent the overgrowth of basidiomycetes by moulds.
You need to consider other solutions. First of all, is the barn
facility to be used as a growing room only? If so, then is it
possible to build a clean "culture" or spawn room elsewhere, under
conditions that can be more tightly controlled. Generally once a
substrate material colonized by the mushroom that you want to
grow, the desired fungus can outcompete moderate, incidental mould
contamination, as long as growth parameters are optimized for the
former. Even if the barn is to be used as a growing room, all of the
exposed surfaces (presumably wood) need to be cleaned and sealed. As
long as the surfaces are not hosting a substantial amount of fungal
growth (e.g. by Cladosporium, Alternaria, etc.), then these steps
could be accomplished using one or two applications of a penetrating
sealant, such as a shellac-primer. Concrete surfaces should be
sealed as well. Also, an HVAC system with high efficiency filtration
should be installed, and the interior air space maintained under
positive pressure to limit the ingress of contaminant outdoor spores.
Following each crop cycle, the room will need to be meticulously
cleaned, walls and all, due to the high humidity required for
cropping the the propensity for microfungi to colonize even sealed
wall surfaces. Paul Stamets authored a good introductory text which
addresses many of these issues called "The Mushroom Cultivator".
Good luck.
James
> Date: Thu, 2 Dec 1999 12:33:43 -0800
> Reply-to: A Biosafety Discussion List
> From: "Wolz, Sarah"
> Subject: Mushroom farm decon
> To: BIOSAFTY@MITVMA.MIT.EDU
> Somewhat related to biosafety....
>
> I was asked recently how one would go about decontaminating a barn (!) that
> is used to propagate food-grade mushrooms. Apparently the mushroom farmers
> have a contamination problem with the wrong type of fungal spore invading
> their mushroom cultures. They want to empty the barn, fog with something,
> and then reintroduce their cultures under more isolated conditions. What
> would you all recommend as a decon agent to "fog" a mushroom growing barn??
> My last mycology class was longer ago that I can believe...
>
> Thanks!
>
> Sarah Wolz
> Pathogenesis Corp.
> swolz@
>
=========================================================================
Date: Sun, 5 Dec 1999 11:16:09 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Looking for a Christmas gift??
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
For those still looking for a different Christmas gift......
Edward Krisiunas, MT(ASCP), CIC, MPH
Director
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
Disease Neckwear: the Unique Holiday Gift of 1999
ATLANTA--(BUSINESS WIRE)--Dec. 2, 1999--Holiday shoppers this season can
spread awareness about the threat of infectious and organic diseases by
purchasing disease neckties available through TheFrontLine, the new Web site
of the CDC Foundation (). These 100 percent silk ties
make a bold fashion statement, depicting actual cells of a disease in bright
colors -- and featuring information about the disease on the back of the tie.
Five different diseases are depicted on the ties: cholera (burgundy tie with
flecks of pink and cells of blue), Ebola (black tie with red and blue cells
and specks of orange and purple), staphylococcus (brown and olive tie with
gold and yellow cells), tuberculosis (red tie with blue cells and yellow and
orange specks) and malaria (a malarial mixture of red and green).
The disease ties are distributed through the SHARE store at the Centers for
Disease Control and Prevention (CDC) and sell for $35 apiece, plus shipping
and handling. A portion of proceeds goes to the fight against deadly disease.
The CDC Foundation is a nonprofit enterprise that fights threats to health by
developing programs with the CDC. To locate the disease neckties on the CDC
Foundation Web site, click on the "Get involved!" link.
CONTACT:
CDC Foundation, Atlanta
Michael Baxter, 404-653-0790
mbaxter@
KEYWORD: GEORGIA
=========================================================================
Date: Mon, 6 Dec 1999 13:36:01 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Leonard, Thomas"
Subject: NRM Fails
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
I recently passed the Specialist Microbiologist examination offered by the
National Registry of Microbiologists (NRM). Today, I called the NRM to
request a written copy of my scores. I was informed that the NRM is not
providing scores for those who passed the examination this year. Evidently,
scores were provided in the past, and they intend to provide scores in the
future, just not this year. This year, those who failed the examination
received scoring breakdowns, but those who passed the examination only
received notification that they passed.
Say What?! What kind of second rate organization are we dealing with here?!
Don't most other certifying bodies provide test results? I paid the
extortion fee to take the exam--the least they can do is provide me with a
copy of my score! I intend to write a scathing letter to the NRM to bring
the ineptness of this policy to their attention. If you passed the
examination this year and are interested in your score, I urge you to do
the same.
Bitter ;-(
********************************
R. Thomas Leonard, M.S.,CSP,CBSP
Safety Officer
The Wistar Institute
3601 Spruce Street
Philadelphia, PA 19104
tleonard@wistar.upenn.edu
Ph:215-898-3712
Fx:215-898-3868
********************************
=========================================================================
Date: Tue, 7 Dec 1999 14:03:51 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Dan Shawler
Subject: Employees with Immune Disorders
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
We have a new worker in the laboratory who has multiple schlerosis (MS), an
autoimmune disease. What, if any, precautions and/or restrictions should be
legally placed on his laboratory activities. Can anyone offer a realisitic
risk assessment for someone with this disease working with animal tissue
culture and serologic assays (of animal sera). What are the implications if
he should start working on procedures involving human specimens covered by
the Bloodborne Pathogen Control Standards?
Any input will be appreciated.
Dan Shawler
Safety Officer
Sidney Kimmel Cancer Center
=========================================================================
Date: Wed, 8 Dec 1999 09:46:46 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Bernholc, Nicole M"
Subject: Re: Employees with Immune Disorders
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
It really depends on the level of his MS and how it effects him (legs, arms,
etc) This would have to be evaluated I imagine and then review what he/she
is working with and whether it would be hazardous to if dropped to the
individual and the general lab. They may have restricted use to a hood.
Personal protective equipment will be a must.
=========================================================================
Date: Wed, 8 Dec 1999 12:25:37 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: desperately seeking answers!
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Some of you responded a while back---still looking for consultants on
FDA/cGMP/cGLP facilities and issues. I only had a few answers come back to
me. I would appreciate hearing from those of you who have had to deal in
this area, particularly in vector preparation labs.
thanks
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
=========================================================================
Date: Wed, 8 Dec 1999 16:23:19 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Senjem, David H."
Subject: Medical Waste Disinfection
MIME-Version: 1.0
Content-Type: text/plain
Is anyone on the list involved in or knowledgeable of situations where
medical waste is managed through a chemical disinfection process? If so, I
am wondering about testing procedures to ensure the efficacy of the
disinfection procedure. Are there commercial spore suspensions available or
should one plan on preparing their own onsite? What constitutes effective
disinfection? We are currently working with a vendor on medical plastics
recycling and want to ensure that appropriate disinfection has occurred.
Any suggestions would be appreciated.
David H. Senjem
Biosafety Officer
Mayo Clinic
Rochester, MN
(507) 284-8890
=========================================================================
Date: Thu, 9 Dec 1999 09:01:19 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Robert N. Latsch"
Subject: Re: Medical Waste Disinfection
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
If this is a recycling operation why not have the materials autoclaved?
This will accomplished two things.
Sterilisation of the materials.
Compressing the plastic.
This will allow for more items to be collected in the same space.
Bob
>
>Is anyone on the list involved in or knowledgeable of situations where
>medical waste is managed through a chemical disinfection process? If so, I
>am wondering about testing procedures to ensure the efficacy of the
>disinfection procedure. Are there commercial spore suspensions available or
>should one plan on preparing their own onsite? What constitutes effective
>disinfection? We are currently working with a vendor on medical plastics
>recycling and want to ensure that appropriate disinfection has occurred.
>Any suggestions would be appreciated.
>
>David H. Senjem
>Biosafety Officer
>Mayo Clinic
>Rochester, MN
>(507) 284-8890
________________________________________________
__ / _______________________________________________
_ \ / /Robert N. Latsch USSF State Referee 6
\ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
\ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
\ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Thu, 9 Dec 1999 09:31:53 -0500
Reply-To: "drobbins@umaryland.edu"
Sender: A Biosafety Discussion List
From: "DEANNA S. ROBBINS"
Subject: Re: List Owner: Sending mail
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Hello Richard, Help! Of course I have misplaced my info on the Biosafty
mail list. I need to remove myself from the list for about 3 weeks and then
start up again at a new e-mail address. Could you be so kind as to send me
the instuctions again? Sorry for the trouble.
Have a Happy Holiday ! -Deanna
Deanna S. Robbins, Ph.D.
Dept. of Veterans Affairs Medical Center
10 N. Greene Street
Baltimore, MD 21202
-----Original Message-----
From: Richard Fink [SMTP:rfink@MIT.EDU]
Sent: Friday, October 29, 1999 11:00 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: List Owner: Sending mail
As mailing programs get more sophisticated they are able to send "styled"
text (i.e.: color, different fonts, html, etc.). However, older mail
programs and even new "primative" mail programs cannot receive this type of
information. I am currently using Eudora Pro 4.0.2 and had been sending
only styled text which not everyone could read. I have reset the options
to send both styled and plain text, thus folks who can only receive plain
text can read my messages and those who can interpret styled text will
receive it "in style" (sorry bad pun).
So, if you want everyone to be able to read you messages, please review the
settings in your mail program and make changes as needed.
Thank you,
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
=========================================================================
Date: Thu, 9 Dec 1999 10:43:52 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: desperately seeking answers!
MIME-Version: 1.0
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I am now in the same boat as Theresa, desperately seeking information on
FDA/cGMP/cGLP. Theresa, could you please forward to me any information
which you have gotten? Thanks!
Janice Flesher, MS, CBSP
Principle Industrial Hygienist/Biosafety Officer
EOHSS - University Medical Dental School of NJ
97 Paterson St. #227
New Brunswick, NJ, 08901
(732) 235-8497 phone
(732) 235-8499 fax
fleshejk@umdnj.edu
-----Original Message-----
From: Therese M. Stinnett
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Wednesday, December 08, 1999 2:27 PM
Subject: desperately seeking answers!
>Some of you responded a while back---still looking for consultants on
>FDA/cGMP/cGLP facilities and issues. I only had a few answers come back to
>me. I would appreciate hearing from those of you who have had to deal in
>this area, particularly in vector preparation labs.
>
>thanks
>
>
>Therese M. Stinnett
>Biosafety Officer
>Health and Safety Division
>UCHSC, Mailstop C275
>
>Phone: 303-315-6754
>Pager: 303-266-5402
>Fax: 303-315-8026
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=========================================================================
Date: Thu, 9 Dec 1999 10:54:53 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Alain Garnier
Subject: Re: desperately seeking answers!
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
That makes 3: I would also be interested in this information! So anyone
that can help, send any information to me too.
Thanks.
At 10:43 99-12-09 -0500, you wrote:
>I am now in the same boat as Theresa, desperately seeking information on
>FDA/cGMP/cGLP. Theresa, could you please forward to me any information
>which you have gotten? Thanks!
>
>Janice Flesher, MS, CBSP
>Principle Industrial Hygienist/Biosafety Officer
>EOHSS - University Medical Dental School of NJ
>97 Paterson St. #227
>New Brunswick, NJ, 08901
>(732) 235-8497 phone
>(732) 235-8499 fax
>fleshejk@umdnj.edu
>
>
>-----Original Message-----
>From: Therese M. Stinnett
>To: BIOSAFTY@MITVMA.MIT.EDU
>Date: Wednesday, December 08, 1999 2:27 PM
>Subject: desperately seeking answers!
>
>
>>Some of you responded a while back---still looking for consultants on
>>FDA/cGMP/cGLP facilities and issues. I only had a few answers come back=
to
>>me. I would appreciate hearing from those of you who have had to deal in
>>this area, particularly in vector preparation labs.
>>
>>thanks
>>
>>
>>Therese M. Stinnett
>>Biosafety Officer
>>Health and Safety Division
>>UCHSC, Mailstop C275
>>
>>Phone: 303-315-6754
>>Pager: 303-266-5402
>>Fax: 303-315-8026
>
>=20
Alain Garnier
Professeur adjoint=05
D=E9partement de g=E9nie chimique, 3362 Pouliot
Universit=E9 Laval
Ste-Foy, Quebec, Canada, G1K 7P4
tel: 418-656-3106
fax: 418-656-5993
e-mail: alain.garnier@gch.ulaval.ca
=========================================================================
Date: Mon, 27 Aug 1956 21:59:56 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Al Jin
Subject: Re: Medical Waste Disinfection
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
David,
To add to Bob's comment, the chemical or physical disinfection method is
not the main question. The main pivitol question (as I see it) is what
methods are approved by your State (or County) Health Department. Before
investing a lot of time (and money) into selecting a disinfection method,
check with the regulators to see what's acceptable to them.
Alternatively, you may want to start segregating contaminated from
non-contaminated plasticware at the source. You'll be surprised on how much
non-contaminated plastic you can recover.
Alfred Jin, BSO, IH, MS, CBSP, M(ASCP), BSM(ASM), CM(ACM),
Hazards Control Department,
Lawrence Livermore National Laboratory,
7000 East Avenue MS-289, Livermore, CA 94550,
Phone:925 423-7385, Fax:423-1086, ]
Jin2@
>If this is a recycling operation why not have the materials autoclaved?
>This will accomplished two things.
>Sterilisation of the materials.
>Compressing the plastic.
>This will allow for more items to be collected in the same space.
>
>Bob
>>
>>Is anyone on the list involved in or knowledgeable of situations where
>>medical waste is managed through a chemical disinfection process? If so, I
>>am wondering about testing procedures to ensure the efficacy of the
>>disinfection procedure. Are there commercial spore suspensions available or
>>should one plan on preparing their own onsite? What constitutes effective
>>disinfection? We are currently working with a vendor on medical plastics
>>recycling and want to ensure that appropriate disinfection has occurred.
>>Any suggestions would be appreciated.
>>
>>David H. Senjem
>>Biosafety Officer
>>Mayo Clinic
>>Rochester, MN
>>(507) 284-8890
>
>
> ________________________________________________
>__ / _______________________________________________
>_ \ / /Robert N. Latsch USSF State Referee 6
> \ \ / / 27610 Tremiane Dr. USSF Assessor 7 CWRU
> \ \/ / Euclid, Ohio, 44132 High School, Indoor Occupational &
> \ / U.S.A. RA Member Environmental Safety
=========================================================================
Date: Thu, 9 Dec 1999 12:28:44 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Michael Mispagel
Organization: UGA College of Vet. Med
Subject: Re: desperately seeking answers!
MIME-Version: 1.0
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
As a Quality Assurance Manager for GLP studies here, I may be able to
answer some specific question in that arena. Or you may find some
answers at both my webpage at
or the web site of the Society of Quality Assurance at
In addition, you may want to subscribe to the GLPForum list to have
access to numerous QA professionals. Subscribe to GLPForum at
GLPForum@sk.sympatico.ca with the message "subscribe GLPForum
yourname".
If you want the names of some professional GLP consultants, email me
privately and I will see what I can do.
Regards,
-----------------------------------------------------------
Michael E. Mispagel, Ph.D.
Quality Assurance Manager
University of Georgia
College of Veterinary Medicine
Athens GA 30602-7371
(706)542-5875; FAX (706)542-8254
MISPAGEL@CALC.VET.UGA.EDU
-----------------------------------------------------------
=========================================================================
Date: Thu, 9 Dec 1999 18:41:15 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Elizabeth Smith
Subject: Re: Employees with Immune Disorders
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Have the person consult with his/her personal physician, who will be more
likely to be familiar with the individual patient's health concerns (not
only the MS, but other things that would effect the overall health).
You consult with an occupational health physician (someone who is up on the
medical stuff, but is also fluent with occupational health issues and will
understand the work situation.
Do a personal risk assessment, and have input from both physicians.
Depending on labor law - is it ultimately the employee's decision or the
employer's?
It's a question which has arisen here in another form - what if someone
won't produce an "adequate" antibody level upon vaccination - are they
immune compromised (for that specific organism)? Should you let them work
with it? We haven't found a one-size-fits-all answer (not even a
one-size-fits-most).
Elizabeth E. Smith
Environmental, Health & Safety Manager
BioPort Corporation
3500 N. Martin L. King Blvd.
Lansing, Michigan 48906
517-327-6806
There is nothing to learn from history but that men do not learn from
history. - Hegel
__________________________________________________
Do You Yahoo!?
Thousands of Stores. Millions of Products. All in one place.
Yahoo! Shopping:
=========================================================================
Date: Tue, 14 Dec 1999 12:44:29 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Jean.Goldberg"
Subject: Protocol for Recombinant Adenoviral Shedding
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; CHARSET=US-ASCII
Thanks for your responses to my previous question on the
rabbits/recombinant adenovirus/ABSL1 vs ABSL2. I am
writing as a follow-up to that question. My researcher is
confident he can provide assay results to will demonstrate
that rabbits treated with a replication deficient
recombinant adenovirus will not shed wild-type or
recombinant adenovirus. My question is this: Do other
institutions have standardized protocols (including number
of samples of days on which samples are collected) for
collecting and analyzing such samples? If so, can you
provide me with a copy of your protocols? If not, how do
you approach this issue. Thanks in advance for your
assistance. -- Jean
----------------------------------------
Jean Goldberg
Email: Jean.Goldberg@Med.Nyu.Edu
"NYU Medical Center"
=========================================================================
Date: Tue, 14 Dec 1999 14:00:30 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Petty, Carol"
Subject: Ceiling materials for BSL-3 Labs
MIME-Version: 1.0
Content-Type: text/plain
Presently, we are in the midst of constructing a BSL-3 laboratory. Does
anyone know of a company that manufactures drop ceiling panels that can be
sealed sufficiently for this application. Please let me know the name,
address, and phone number if you know. Thanks.
Carol L. Petty, C.I.H.
Industrial Hygienist
Phone: (505) 845-1076
Fax: (505) 845-1174
email: cpetty@
=========================================================================
Date: Tue, 14 Dec 1999 15:36:16 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Clifford R. Roberts DVM"
Subject: Re: Ceiling materials for BSL-3 Labs
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
We are using panels sold by Life Science Products, at 800-638-9874.
The installation includes a very sustantial fiber reinforced plastic
grid, gaskets, and fiberglass-foam sandwich panels.
Personally, I would prefer a sealed ceiling with gasketed/sealed
access panels using the same fiberglass composite material.
Cliff
>Presently, we are in the midst of constructing a BSL-3 laboratory. Does
>anyone know of a company that manufactures drop ceiling panels that can be
>sealed sufficiently for this application.
>Carol L. Petty, C.I.H.
>Industrial Hygienist
>Phone: (505) 845-1076
>Fax: (505) 845-1174
>email: cpetty@
Clifford R. Roberts D.V.M.
Director ucivet@uci.edu
University Laboratory Animal Resources
147BSA 1310 949-824-4340
University of California, Irvine Fax 949-824-2003
Irvine, CA 92697-1310
=========================================================================
Date: Wed, 15 Dec 1999 10:36:12 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Robin Newberry
Subject: Enterococcus faecalis and E. faecium
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
I'm having some difficulty in pinning down an appropriate BSL for
working with E. faecalis and E. faecium; I've a researcher proposing
to look for MDR strains of both.
So far I know that Enterococcus faecalis is associated with
intra-abdominal and pelvic infections and can cause endocarditis, and
that E. faecium is listed by the CDC as "... a species virtually
devoid of known overt pathogenic traits but more likely to be
resistant to even antibiotics of last resort."
In his protocol the researcher listed both as being BSL2, but I've
been unable to confirm this. I also suspect that attempts to culture
MDR strains of these oughta be something better than BSL2 anyway.
Can someone point me to a good resource, preferably on the web?
Many TIA,
Robin
W. Robert Newberry, IV CIH, CHMM
Director, Environmental Health and Safety
Clemson University
wnewber@clemson.edu ehs@clemson.edu
=========================================================================
Date: Wed, 15 Dec 1999 10:59:11 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stefan Wagener
Subject: Re: Enterococcus faecalis and E. faecium
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Review the Canadian MSDS's under "Streptococcus faecalis" at:
Streptococcus faecalis and Enterococcus faecalis are the same bug, depending
whom you ask in the world of scientific nomenclature.
Hope this helps.
Stefan :-)
-------------
Stefan Wagener, Ph.D, CBSP
Michigan State University, ORCBS
C-126 Research Complex Engineering
East Lansing, MI 48824
Phone: (517) 355-6503
Fax: (517) 353-4871
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Robin Newberry
Sent: Wednesday, December 15, 1999 10:36 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Enterococcus faecalis and E. faecium
I'm having some difficulty in pinning down an appropriate BSL for
working with E. faecalis and E. faecium; I've a researcher proposing
to look for MDR strains of both.
So far I know that Enterococcus faecalis is associated with
intra-abdominal and pelvic infections and can cause endocarditis, and
that E. faecium is listed by the CDC as "... a species virtually
devoid of known overt pathogenic traits but more likely to be
resistant to even antibiotics of last resort."
In his protocol the researcher listed both as being BSL2, but I've
been unable to confirm this. I also suspect that attempts to culture
MDR strains of these oughta be something better than BSL2 anyway.
Can someone point me to a good resource, preferably on the web?
Many TIA,
Robin
W. Robert Newberry, IV CIH, CHMM
Director, Environmental Health and Safety
Clemson University
wnewber@clemson.edu ehs@clemson.edu
=========================================================================
Date: Wed, 15 Dec 1999 11:11:36 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: Enterococcus faecalis and E. faecium
MIME-Version: 1.0
Content-Type: multipart/mixed;
boundary="----=_NextPart_000_007A_01BF46ED.2732D720"
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Robin,
The Manual of Clinical Microbiology (ASM) has a good section on
Antibiotic -resistant enterocci, including E. faecium. Ab resistant
enterocci have become serious sources of nosocomial infections with the
ability to to be transmitted not only within a hospital but also among
hospitals in cities or states.
I can fax you this section if you don't have easy access.
It doesn't sound like the researcher is constructing the strains so
recombinant DNA guidelines wouldn't apply but it may be appropriate to run
this by your biosafety committee and/or talk to some infectious disease
people.
Janice Flesher, MS, CBSP
Principle Industrial Hygienist/Biosafety Officer
EOHSS - University Medical Dental School of NJ
97 Paterson St. #227
New Brunswick, NJ, 08901
(732) 235-8497 phone
(732) 235-8499 fax
fleshejk@umdnj.edu
-----Original Message-----
From: Robin Newberry
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Wednesday, December 15, 1999 10:40 AM
Subject: Enterococcus faecalis and E. faecium
>I'm having some difficulty in pinning down an appropriate BSL for
>working with E. faecalis and E. faecium; I've a researcher proposing
>to look for MDR strains of both.
>
>So far I know that Enterococcus faecalis is associated with
>intra-abdominal and pelvic infections and can cause endocarditis, and
>that E. faecium is listed by the CDC as "... a species virtually
>devoid of known overt pathogenic traits but more likely to be
>resistant to even antibiotics of last resort."
>
>In his protocol the researcher listed both as being BSL2, but I've
>been unable to confirm this. I also suspect that attempts to culture
>MDR strains of these oughta be something better than BSL2 anyway.
>
>Can someone point me to a good resource, preferably on the web?
>
>Many TIA,
>
>Robin
>
>W. Robert Newberry, IV CIH, CHMM
>Director, Environmental Health and Safety
>Clemson University
>
>wnewber@clemson.edu ehs@clemson.edu
>
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=========================================================================
Date: Wed, 15 Dec 1999 11:30:40 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Robin Newberry
Subject: Re: Enterococcus faecalis and E. faecium
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
>It doesn't sound like the researcher is constructing the strains so
>recombinant DNA guidelines wouldn't apply but it may be appropriate to run
>this by your biosafety committee and/or talk to some infectious disease
>people.
I believe (his protocol is rather deficient due to its brevity) that
he is attempting to recover MDR strains from chicken products and
by-products.
Robin
W. Robert Newberry, IV CIH, CHMM
Director, Environmental Health and Safety
Clemson University
wnewber@clemson.edu ehs@clemson.edu
=========================================================================
Date: Wed, 15 Dec 1999 14:01:38 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Janice Flesher
Subject: Re: Enterococcus faecalis and E. faecium
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Robin's inquiry is certainly timely. I opened the Dec. ASM News today to
Current Topics and found and article on E. faecium. To summarize, the FDA
is approving a new "last, last" resort antibiotic for MDR strains of E.f.
(Synercid) The first vancomycin resistant strain of E.f was reported in
1989. Currently there are over 20,000 infections and are very difficult to
control in hospitals. Especially at risk are the usual suspects: young
children, elderly, patients receiving chemotherapy, transplants, etc.
There is a problem, however, with the agricultural use of a similar
antibiotic, virginiamycin, routinely fed to poultry. Critics suggest that
this agricultural antibiotic has already jeopardized Synercid's value.
So, once again, us biosaftyers are on the frontline of microbial issues.
Janice Flesher, MS, CBSP
Principle Industrial Hygienist/Biosafety Officer
EOHSS - University Medical Dental School of NJ
97 Paterson St. #227
New Brunswick, NJ, 08901
(732) 235-8497 phone
(732) 235-8499 fax
fleshejk@umdnj.edu
-----Original Message-----
From: Robin Newberry
To: BIOSAFTY@MITVMA.MIT.EDU
Date: Wednesday, December 15, 1999 11:32 AM
Subject: Re: Enterococcus faecalis and E. faecium
>>It doesn't sound like the researcher is constructing the strains so
>>recombinant DNA guidelines wouldn't apply but it may be appropriate to run
>>this by your biosafety committee and/or talk to some infectious disease
>>people.
>
>I believe (his protocol is rather deficient due to its brevity) that
>he is attempting to recover MDR strains from chicken products and
>by-products.
>
>Robin
>
>W. Robert Newberry, IV CIH, CHMM
>Director, Environmental Health and Safety
>Clemson University
>
>wnewber@clemson.edu ehs@clemson.edu
>
=========================================================================
Date: Wed, 15 Dec 1999 15:04:53 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Enterococcus faecalis and E. faecium
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
At 10:36 AM 12/15/99 -0500, W. Robert Newberry, IV CIH, CHMM
wrote:
>I'm having some difficulty in pinning down an appropriate BSL for
>working with E. faecalis and E. faecium; I've a researcher proposing
>to look for MDR strains of both.
Robin,
For the moment ignore the fact that the isolates will be MDR. Consider: 1)
how
are these agents transmitted; 2) how pathogenic are they; 3) how many of the
bugs will the researcher be playing with; 4) will the procedure(s) likely to
result in a significant aerosol; 5) will animals be inoculated. Each of these
points need to be answered inorder to come up with a risk assessment. This
will get you a base line biosafety level. After getting this baseline you add
in the MDR factor and consider whether this has a significant affect on the
risk assessment.
Transmission - fecal-oral
Pathogenicity - low to a normal healthy adult
So we are at the border between BL1 and BL2. The research protocol is
critical
to the rest of the risk assessment.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Thu, 16 Dec 1999 09:57:03 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Cipriano,Mary"
Subject: Re: Protozoan - Encephlitozoon cuniculi
Mime-Version: 1.0
Content-Type: text/plain; charset=iso-8859-1
Content-Transfer-Encoding: quoted-printable
Content-Transfer-Encoding: quoted-printable
Does anyone know anything about E. cuniculi, with regards to whether i=
t is
zoonotic, or any other information. It is fairly common in rabbits from=
what
I've heard. We've had a rabbit colony outside of the US that has just h=
ad an
outbreak and we are trying to check into. Any other sources of info. wo=
uld be
appreciated. You can reply to me directly. Don't suppose that this is a=
high
interest item!!
Thanks,
Mary Cipriano
mary.cipriano@
=
=========================================================================
Date: Thu, 16 Dec 1999 10:57:05 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "J.H. Keene"
Subject: Re: Enterococcus faecalis and E. faecium
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: 7bit
Robin, E/S faecalis is a known moderate risk pathogen and is, in the US,
classified as a BL-2 agent. The fact that it has multiple antibiotic
resistance is not applicable to the Biosafety Level. The organism does not
change the way it is transmitted because of it's antibiotic resistance and
the risk of occupational acquisition therefore does not change. The
treatment of it, if it is acquired, would be different, but choosing the
correct antibiotic would result in treatment just as if it was not
resistant. I would suggest that strict BL-2 procedures be used, and that
when the strains are isolated, some attempt at antibiotic sensitivity be
performed to provide information on the type of drugs that would work if
someone were to get infected. Also remember that most of the E. faecalis
infections are a result of "self contamination" of immune compromised
individuals. By immune compromised I don't necessarily mean those that are
on chemo or have had treatments that compromise their immune systems, but
also those that have had surgery, etc. These people have usually been on a
course of some type of antibiotics that have selected for antibiotic
resistance of the normal flora. The other type of transmission is
nosocomial, i.e. from one patient to another by virtue of the use of
contaminated equipment (catheters, etc.) or personnel (hands/gloves).
----- Original Message -----
From: Robin Newberry
To:
Sent: Wednesday, December 15, 1999 10:36 AM
Subject: Enterococcus faecalis and E. faecium
> I'm having some difficulty in pinning down an appropriate BSL for
> working with E. faecalis and E. faecium; I've a researcher proposing
> to look for MDR strains of both.
>
> So far I know that Enterococcus faecalis is associated with
> intra-abdominal and pelvic infections and can cause endocarditis, and
> that E. faecium is listed by the CDC as "... a species virtually
> devoid of known overt pathogenic traits but more likely to be
> resistant to even antibiotics of last resort."
>
> In his protocol the researcher listed both as being BSL2, but I've
> been unable to confirm this. I also suspect that attempts to culture
> MDR strains of these oughta be something better than BSL2 anyway.
>
> Can someone point me to a good resource, preferably on the web?
>
> Many TIA,
>
> Robin
>
> W. Robert Newberry, IV CIH, CHMM
> Director, Environmental Health and Safety
> Clemson University
>
> wnewber@clemson.edu ehs@clemson.edu
>
>
=========================================================================
Date: Thu, 16 Dec 1999 11:29:47 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stefan Wagener
Subject: Biosafety level and antibiotic resistance???
In-Reply-To:
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
Hi Jack;
I appreciated your response. It was interesting to me that you state that
"the fact that it has multiple antibiotic resistance is not applicable to
the Biosafety Level". I am not sure where you get this, however if that's
true, then the logical consequence is that the CDC/BMBL promoted biosafet=
y
level approach is outdated and it is time to move on to a national risk
group classification which by definition takes treatability into account.
Out of such a risk group classification system would then naturally emerg=
e
an appropriate containment system which is not limited to routes of
transmission but risk to personnel and available treatment options.
Let=92s work on something like this and take the rest of the world (which=
is
using a risk group classification) as a template.
My 2 cents worth of trouble :-)
Stefan
Stefan Wagener, Ph.D, CBSP
Michigan State University, ORCBS
C-126 Research Complex Engineering
East Lansing, MI 48824
Phone: (517) 355-6503
Fax: (517) 353-4871
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of J.H. Keene
Sent: Thursday, December 16, 1999 10:57 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: Enterococcus faecalis and E. faecium
Robin, E/S faecalis is a known moderate risk pathogen and is, in the US,
classified as a BL-2 agent. The fact that it has multiple antibiotic
resistance is not applicable to the Biosafety Level. The organism does n=
ot
change the way it is transmitted because of it's antibiotic resistance an=
d
the risk of occupational acquisition therefore does not change. The
treatment of it, if it is acquired, would be different, but choosing the
correct antibiotic would result in treatment just as if it was not
resistant. I would suggest that strict BL-2 procedures be used, and that
when the strains are isolated, some attempt at antibiotic sensitivity be
performed to provide information on the type of drugs that would work if
someone were to get infected. Also remember that most of the E. faecalis
infections are a result of "self contamination" of immune compromised
individuals. By immune compromised I don't necessarily mean those that a=
re
on chemo or have had treatments that compromise their immune systems, but
also those that have had surgery, etc. These people have usually been on=
a
course of some type of antibiotics that have selected for antibiotic
resistance of the normal flora. The other type of transmission is
nosocomial, i.e. from one patient to another by virtue of the use of
contaminated equipment (catheters, etc.) or personnel (hands/gloves).
----- Original Message -----
From: Robin Newberry
To:
Sent: Wednesday, December 15, 1999 10:36 AM
Subject: Enterococcus faecalis and E. faecium
> I'm having some difficulty in pinning down an appropriate BSL for
> working with E. faecalis and E. faecium; I've a researcher proposing
> to look for MDR strains of both.
>
> So far I know that Enterococcus faecalis is associated with
> intra-abdominal and pelvic infections and can cause endocarditis, and
> that E. faecium is listed by the CDC as "... a species virtually
> devoid of known overt pathogenic traits but more likely to be
> resistant to even antibiotics of last resort."
>
> In his protocol the researcher listed both as being BSL2, but I've
> been unable to confirm this. I also suspect that attempts to culture
> MDR strains of these oughta be something better than BSL2 anyway.
>
> Can someone point me to a good resource, preferably on the web?
>
> Many TIA,
>
> Robin
>
> W. Robert Newberry, IV CIH, CHMM
> Director, Environmental Health and Safety
> Clemson University
>
> wnewber@clemson.edu ehs@clemson.edu
>
>
=========================================================================
Date: Thu, 16 Dec 1999 09:59:03 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: Re: Biosafety level and antibiotic resistance???
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Stefan wrote:
>It was interesting to me that you state that "the fact that it has =
multiple
>antibiotic resistance is not applicable to the Biosafety Level"...the
>CDC/BMBL promoted biosafety level approach is outdated and it is time =
to
>move on to a national risk group classification which by definition =
takes
>treatability into account.
I would argue that in fact the NIH/CDC risk groups do in fact take
treatability into account. Risk group 2 is for those organisms that =
are
known to cause disease but are TREATABLE or for which there is a =
vaccine.
BSL2 contains those organisms, depending upon route of infection.
my trouble-making for the day!
Therese M. Stinnett=20
Biosafety Officer=20
Health and Safety Division=20
UCHSC, Mailstop C275
4200 E. 9th Ave.
Denver, CO 80262
Phone:=A0 303-315-6754=20
Pager:=A0=A0 303-266-5402=20
Fax:=A0=A0=A0=A0=A0 303-315-8026=20
=========================================================================
Date: Thu, 16 Dec 1999 12:07:14 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Leonard, Thomas"
Subject: Re: Enterococcus faecalis and E. faecium
In-Reply-To:
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You said:
>The fact that it has multiple antibiotic resistance is not >applicable to
the Biosafety Level.
See page 11 of BMBL under Biosafety Levels. "When specific information is
available to suggest that virulence, pathogenicity, ANTIBIOTIC RESISTANCE
PATTERNS, vaccine, and treatment availability, or other factors are
significantly altered, more (or less) stringent practices may be specified."
>The organism does not change the way it is transmitted because of >it's
antibiotic resistance and the risk of occupational acquisition >therefore
does not change. The treatment of it, if it is acquired, >would be
different, but choosing the correct antibiotic would result >in treatment
just as if it was not resistant.
True. Based on antibiotic resistance, the risk of acquisition may not
change, but the CONSEQUENCES of acquisition may. The consequences of
transmission are at the heart of determining preventative efforts (or
biosafety level), so it is a factor.
********************************
R. Thomas Leonard, M.S.,CSP,CBSP
Safety Officer
The Wistar Institute
3601 Spruce Street
Philadelphia, PA 19104
tleonard@wistar.upenn.edu
Ph:215-898-3712
Fx:215-898-3868
********************************
=========================================================================
Date: Thu, 16 Dec 1999 12:10:49 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "J.H. Keene"
Subject: Re: Enterococcus faecalis and E. faecium
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I'm glad to see my comments bring stimulating response. I responded
directly to Stefan, but thought the rest of the list ought to also see my
response. Just my two cents worth.
I would agree that these things
should probably be taken into account when doing the "risk assessment" and
the provision of treatment should be included in that risk assessment with
regard to what would happen if an accident occurred ( reactive approach).
However, my concern is that we can get to the point of losing site of the
forest because of the number of trees. The best approach to protection of
personnel is developing engineering controls, procedures and personal
protective equipment to "Break the chain of transmission" (pro-active). I
think a lot of folks that don't have a lot of experience in infectious
disease and microbiology get hung up on the "resistance" of the organism and
want to have stricter precautions but don't understand that the mechanism of
transmission is the same no matter the resistance. We have to emphasize the
appropriate procedures and containment for the means of transmission. If we
require even stricter procedures, we make it more difficult to work with the
agents and, at least in some cases, increase the possibility of either not
following the procedures, or making misteaks.
Certainly, the resistance of the organism should be considered and the
personnel warned of the fact that they are working with a resistant agent.
Wherever possible the current status of the resistance should be monitored
and appropriate antibiotics provided where necessary. In many cases there
are antibiotics which, in an emergency would work, but would not be
prescribed under ordinary circumstances for a number of reasons.
----- Original Message -----
From: Leonard, Thomas
To:
Sent: Thursday, December 16, 1999 12:07 PM
Subject: Re: Enterococcus faecalis and E. faecium
> You said:
> >The fact that it has multiple antibiotic resistance is not >applicable to
> the Biosafety Level.
>
> See page 11 of BMBL under Biosafety Levels. "When specific information is
> available to suggest that virulence, pathogenicity, ANTIBIOTIC RESISTANCE
> PATTERNS, vaccine, and treatment availability, or other factors are
> significantly altered, more (or less) stringent practices may be
specified."
>
>
> >The organism does not change the way it is transmitted because of >it's
> antibiotic resistance and the risk of occupational acquisition >therefore
> does not change. The treatment of it, if it is acquired, >would be
> different, but choosing the correct antibiotic would result >in treatment
> just as if it was not resistant.
>
> True. Based on antibiotic resistance, the risk of acquisition may not
> change, but the CONSEQUENCES of acquisition may. The consequences of
> transmission are at the heart of determining preventative efforts (or
> biosafety level), so it is a factor.
>
>
>
>
> ********************************
> R. Thomas Leonard, M.S.,CSP,CBSP
> Safety Officer
> The Wistar Institute
> 3601 Spruce Street
> Philadelphia, PA 19104
> tleonard@wistar.upenn.edu
> Ph:215-898-3712
> Fx:215-898-3868
> ********************************
>
=========================================================================
Date: Thu, 16 Dec 1999 12:56:24 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Norman, Randy"
Subject: Re: Biosafety level and antibiotic resistance???
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>I would argue that in fact the NIH/CDC risk groups do in fact take
>treatability into account.
I agree wholeheartedly. I've been instructing our employees for the past 12
years that antibiotic resistance is one of the classic examples of
agent-specific factors that influences risk assessment.
Randy Norman
Safety Specialist Sr.
BioReliance Corporation
Rockville, MD 20850
Rnorman@
"Success is a journey, not a destination" - Ben Sweetland
=========================================================================
Date: Thu, 16 Dec 1999 13:34:04 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stefan Wagener
Subject: Re: Biosafety level and antibiotic resistance???
In-Reply-To:
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Wonderful, you have my full support. The fact of the matter is that there
are NO CDC risk groups. The CDC BMBL does not endorse a risk group
classification. The only risk group classification currently in use in the
US are part of the NIH rDNA guidelines. Those risk groups and associated
classification are not accepted by FDA, DOT, CDC etc...
That's the whole point and not the reason for the season.
Stefan :-)
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Norman, Randy
Sent: Thursday, December 16, 1999 12:56 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Re: Biosafety level and antibiotic resistance???
>I would argue that in fact the NIH/CDC risk groups do in fact take
>treatability into account.
I agree wholeheartedly. I've been instructing our employees for the past 12
years that antibiotic resistance is one of the classic examples of
agent-specific factors that influences risk assessment.
Randy Norman
Safety Specialist Sr.
BioReliance Corporation
Rockville, MD 20850
Rnorman@
"Success is a journey, not a destination" - Ben Sweetland
=========================================================================
Date: Thu, 16 Dec 1999 15:56:09 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: Biosafety level and antibiotic resistance???
Mime-Version: 1.0
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This is a good discussion to have and it really points out a valid difference of
opinion among the professionals in the field that I think the CDC should
consider. So I'll ring in with mine.
Practically speaking, having risk groups delineated by some government authority
makes our jobs less of a liability. If we are only to decide how best to stop
transmission (i.e. selection of BL1, 2, etc.) then we can assume that our only
charge is to do just that - stop transmission by using the CDC's performance
standards. This is a zero tolerance approach to transmission. In reality, we
can never ensure any set of facilities, equipment, or practices (even those
designed by the CDC) will block transmission 100% of the time. Therefore, I
feel risk groups - i.e. a rating of the consequences of the failure to block
transmission - should always be taken into account when designing the
containment criteria for an experiment.
Without risk group classification added to other information such as antibiotic
resistance, availability of treatment, immune status of the participants, etc.,
our risk assessments will vary widely depending on our perspectives and not on
established scientific fact. For example; to an oncologist treating a patient
for stage 3 lung cancer, a bladder infection may not be a big safety risk. But
to a lab tech processing that cancer patient's samples, it is. The lab tech may
want to do something equivalent to BL2 or 3, while the oncologist may want to
take no precautions at all and may think - 'why are they worrying about getting
it on them, it's not going to kill anyone'. If left to their own devises, these
two would design entirely different safety approaches based on two very
different subjective risk assessments.
That's a problem I often encounter. So I use any risk group classification I
can find to design and justify containment criteria. I use them to decide how
many physical barriers I do or don't need to put between the agent and the
potentially infected parties. The more dire the consequences of infection (as
per others' risk classifications), the more physical barriers I stack into the
system. I don't base my design criteria on how dangerous I, a staff member, or
one of my colleagues thinks it may or may not be. I use the risk group, look at
the corresponding biosafety level, consider any additional characteristics that
could add or detract from the severity of the consequences of infection or
environmental release, then add or subtract barriers from the corresponding
biosafety level to compensate accordingly.
It's a process. I won't always be right. I will sometimes fail and people will
be exposed. I'll over shoot it sometimes and cost the company more money than
they should have spent. But I can give the process due diligence. And that's
the best I can do.
Judy Pointer MS, CBSP
Biosafety Officer
University of Texas MD Anderson Cancer Center
Houston, TX
=========================================================================
Date: Thu, 16 Dec 1999 16:34:48 -0600
Reply-To: louann.burnett@vanderbilt.edu
Sender: A Biosafety Discussion List
From: LouAnn Burnett
Subject: Re: Biosafety level and antibiotic resistance???
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The timing of these list discussions is amazing. I have been putting
together a risk assessment for a project with antibiotic-resistant
_Staphylococcus aureus_ this week. I had independently come to the
conclusion that the risk group descriptions were the most understandable way
to support my discussion when presented to an audience of hospital and
animal care personnel. It's been great to have email after email today
support that perspective. Thanks!
LouAnn
LouAnn Crawford Burnett
Biosafety Program Manager
Vanderbilt University Environmental Health and Safety
Nashville, Tennessee
615/322-0927 (office)
louann.burnett@vanderbilt.edu
=========================================================================
Date: Fri, 17 Dec 1999 08:40:09 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: ABSA Web Site
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
A request from the ABSA Communications Committee. Please direct all comments
to JohnsonB@saic- and not to the list (i.e. keep your finger off of
the
reply button). Thank you.
>From: "Johnson, Barbara"
>To: "'rfink@mit.edu'"
>Subject: ABSA Web
>Date: Thu, 16 Dec 1999 16:11:45 -0500
>X-Mailer: Internet Mail Service (5.5.1960.3)
>
>Hi Richie,
>
>I have a favor to ask you. Would you put the following message on
>Biosafty for the Communications Committee?
>
>"If you haven't visited the ABSA Web Site lately, please stop by and
>check out our new facelift. We are under new management now, and are
>committed to bring you up to date information on Biosafety Information,
>Conferences, and tools for everyday use. We will be expanding into new
>topics in the coming weeks and months, so stop by, see what's new, and
>let us know what you like, don't like, and want more of. Happy Holidays
>from ABSAs Communications Committee."
>
>Barbara Johnson, PhD, RBP
>SAIC-TRSC (703) 326-1004
>
Richard Fink, SM(NRM), CBSP
Biosafty List Owner
rfink@mit.edu
=========================================================================
Date: Fri, 17 Dec 1999 10:46:49 -0600
Reply-To: "mkinsey@"
Sender: A Biosafety Discussion List
From: Melina Kinsey
Organization: MRI
Subject: Re: Biosafety Professional Position Advertisement
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
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Ms. Cook-
I have archived your Biosafety Professional request. I have an opportunity to
transfer within my company to another Biosafety position in Florida.
Currently, I am the Biosafety officer in our Kansas City office and provide
the type of duties you have described. I am wondering if you would be willing
to let me know what your salary range was for the position offered. This will
help me with my negotiations.
Thanks in advance for your help.
Melina Kinsey
Biosafety Officer
Midwest Research Institute
425 Volker Blvd.
Kansas City, MO 64110
(816) 753-7600 x1424
mkinsey@
-----Original Message-----
From: Irene Cooke [SMTP:i-cooke@UIUC.EDU]
Sent: Monday, November 29, 1999 11:18 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Biosafety Professional Position Advertisement
Biological Safety Professional
University of Illinois at Urbana-Champaign
The Division of Environmental Health and Safety is seeking an individual to
assist in a program that includes all aspects of biological safety with
particular emphasis on research activities involving recombinant DNA molecules
and biohazardous agents. This is a full-time academic professional position
starting as soon as possible after the closing date. The individual will work
under the direction of the Assistant Director, Environmental Health and Safety,
Biological Safety Section. An advanced degree in microbiology, cellular
biology, biochemistry, genetics, botany, molecular biology, or related field
strongly preferred. Minimum requirement of a B.S. in any one of the above
areas with relevant professional experience in the field of biosafety
required. Special consideration will be given to candidates with experience in
plant systems and recombinant DNA techniques. Ability to communicate
effectively orally and in writing, and to work as a member of a health and
safety team is required. The position requires the use of computer word
processing and database applications. To ensure full consideration, candidates
should submit a letter of application, a resume, a statement of professional
qualifications, and three letters of reference by January 28, 2000. Send
application materials to: Irene Cooke, Assistant Director, Division of
Environmental Health and Safety, 102 Environmental Health and Safety Building,
101 South Gregory Street, Urbana, IL 61801. For additional information, call
217/244-7801 or email i-cooke@uiuc.edu. Salary is commensurate with
qualifications and experience.
The University of Illinois at Urbana-Champaign is an Affirmative Action/Equal
Opportunity Employer.
Irene Cooke, D.V.M., Ph.D.
Head, Biological Safety Section
Division of Environmental Health and Safety
University of Illinois at Urbana-Champaign
Tel #: (217) 244-7801
Fax #: (217) 244-6594 >
=========================================================================
Date: Fri, 17 Dec 1999 11:41:06 -0600
Reply-To: "mkinsey@"
Sender: A Biosafety Discussion List
From: Melina Kinsey
Organization: MRI
Subject: OOPS! Personal message RE: Biosafety Professional Position
Advertisement
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I am so sorry for sending this message to the group. It was meant to be a
private message, but I goofed. Please delete it from your files. I am sorry
for cluttering the list.
I have learned my lesson the hard way about using the reply button. What a
cyber nightmare.
Melina Kinsey
Biosafety Officer
Midwest Research Institute
425 Volker Blvd.
Kansas City, MO 64110
(816) 753-7600 x1424
mkinsey@
=========================================================================
Date: Fri, 17 Dec 1999 12:52:21 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Eric N. Cook"
Subject: Autoclave validation
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We validate autoclaves with B. stearothermophilus spore vials. Recently I
came across an enzyme system which works when autoclave temperatures
deactivate the enzyme. By adding a simple reagent after the run, within
seconds the run can be validated. (At least this is what the manufacturer
claims) My question to the group is, are you aware of any other methods of
autoclave validation? Thanks in advance for you input.
Eric Cook
Asst. Biosafety Officer
Mass. Inst. of Technology
=========================================================================
Date: Sat, 18 Dec 1999 10:03:27 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Al Jin
Subject: Re: Autoclave validation
In-Reply-To:
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Eric,
At LLNL, we use 2 methods to validate steam sterilized loads. For each bag
we use a product call the "Steam Sterilization Integrator Strip" by
BioSure. Their web is at . For each load the more
traditional Bacillus sterarothermophilius spores method is used. We use the
products by SporAmpule.
The "Steam Sterilization Integrator Strip" system uses a steam activated
indicator that is attached to the autoclave bag. The indicator will change
color when the proper temperature is reached and held. Prior to closure,
the biowaste generator adds an appropriate amount of water into the bag (to
facilitate the production of steam) along with the end of strip that is
attached with a 24 inch lead. The lead strip (using gloves) is placed in
the middle of the bag. The bag is sealed with a rubber band and place into
a rack for autoclaving. As the rack fills, or when it's time to do the run,
a SporeAmpule is added.
After each run, the SporeAmpule is collected and incubated per
manufacturers' directions. Each individual "Steam Sterilization Integrator
strip" is collected from each bag by cutting the rubber band and pulling on
the lead. Each strip is observed to a color change. Each stripe is kept in
a log book as part of our recordkeeping requirements. The results of the
SporeAmpule is later added to the log book. Additionally, the weight of
each bag is also recorded into the log book.
In closing, I hope this helps.
Alfred Jin, BSO, IH, MS, CBSP, M(ASCP), BSM(ASM), CM(ACM),
Hazards Control Department, Lawrence Livermore National Laboratory,
7000 East Avenue MS-289, Livermore, CA 94550,
Phone:925 423-7385, Fax:423-1086,
Jin2@
>We validate autoclaves with B. stearothermophilus spore vials. Recently I
>came across an enzyme system which works when autoclave temperatures
>deactivate the enzyme. By adding a simple reagent after the run, within
>seconds the run can be validated. (At least this is what the manufacturer
>claims) My question to the group is, are you aware of any other methods of
>autoclave validation? Thanks in advance for you input.
>
>Eric Cook
>Asst. Biosafety Officer
>Mass. Inst. of Technology
=========================================================================
Date: Fri, 17 Dec 1999 11:33:34 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: Re: Custom Purchasing programs
MIME-Version: 1.0
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Looking for information on any institutions, but especially academia, =
where
you have hired a 3rd party to provide purchasing, shipping and tracking
(financial as well as hazmat inventory) of chemicals, and biotech =
reagents,
as well as consumable/disposables for labs. How has it worked? who are =
you
using? do you have a website for their firm?
thanks
Therese M. Stinnett=20
Biosafety Officer=20
Health and Safety Division=20
UCHSC, Mailstop C275
4200 E. 9th Ave.
Denver, CO 80262
Phone:=A0 303-315-6754=20
Pager:=A0=A0 303-266-5402=20
Fax:=A0=A0=A0=A0=A0 303-315-8026=20
=========================================================================
Date: Fri, 17 Dec 1999 14:31:11 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Paul Zel
Subject: Re: Custom Purchasing programs
In-Reply-To:
MIME-Version: 1.0
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Therese: I've been speaking with a company that is providing this type
of service to several industrial clients in New Jersey. From the
description, there might be an application of the program to an
'academic-like' site as well. The company is Chemcept, Inc. (518)
384-0270. Ask to speak with Dan Ferris, the company's president.
Paul Zel
Director, EH&S
Memorial Sloan-Kettering Cancer Center
New York, N.Y.
(212) 639-7849
zelp@
______________________________ Reply Separator _________________________________
Subject: Re: Custom Purchasing programs
Author: Therese.Stinnett (Therese.Stinnett@UCHSC.EDU) at Internet
Date: 12/17/99 1:33 PM
Looking for information on any institutions, but especially academia, where
you have hired a 3rd party to provide purchasing, shipping and tracking
(financial as well as hazmat inventory) of chemicals, and biotech reagents,
as well as consumable/disposables for labs. How has it worked? who are you
using? do you have a website for their firm?
thanks
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
4200 E. 9th Ave.
Denver, CO 80262
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
=========================================================================
Date: Fri, 17 Dec 1999 13:57:10 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Betty Kupskay
Subject: Re: Autoclave validation
Mime-Version: 1.0
Content-type: text/plain; charset=us-ascii
Hi Eric! Another method that is available is the 3M Attest Rapid Readout
Biological Indicators. This indicator detects the activity of a naturally
occurring B. stearothermophilus enzyme, which is alpha-glucosidase. Detection of
this enzyme indicates a sterilization failure. A final negative biological
indicator result is made after 3 hours. Only one drawback......you have to read
it that day. If you incubate for a longer period of time than 3 hours, you may
get an erroneous result.
Hope this helps!
Betty Kupskay
Biosafety Specialist/Health Canada
Canadian Science Centre for Human and Animal Health
1015 Arlington St., Suite A1010
Winnipeg, MB R3E 3P6
Ph: 204-789-2065
Fax: 204-789-2069
EMail: betty_kupskay@hc-sc.gc.ca
"Eric N. Cook" on 99/12/17 11:52:21 AM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Betty Kupskay)
Subject: Autoclave validation
We validate autoclaves with B. stearothermophilus spore vials. Recently I
came across an enzyme system which works when autoclave temperatures
deactivate the enzyme. By adding a simple reagent after the run, within
seconds the run can be validated. (At least this is what the manufacturer
claims) My question to the group is, are you aware of any other methods of
autoclave validation? Thanks in advance for you input.
Eric Cook
Asst. Biosafety Officer
Mass. Inst. of Technology
=========================================================================
Date: Fri, 17 Dec 1999 13:14:19 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Lauer
Subject: Re: Autoclave validation
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
At 12:52 PM -0500 12/17/99, Eric N. Cook wrote:
>We validate autoclaves with B. stearothermophilus spore vials. Recently I
>came across an enzyme system which works when autoclave temperatures
>deactivate the enzyme. By adding a simple reagent after the run, within
>seconds the run can be validated. (At least this is what the manufacturer
>claims) My question to the group is, are you aware of any other methods of
>autoclave validation? Thanks in advance for you input.
>
>Eric Cook
>Asst. Biosafety Officer
>Mass. Inst. of Technology
For many years we used a chemical indicator, called Thermolog S, that
integrated time, temperature and moisture conditions to validate the
decontamanation of laboratory (culture media) waste in a gravity type
autoclave operating at 121 degrees C. We also conducted studies,
"Decontaminating infectious laboratory waste by autoclaving", Appl.
Environ. Microb.44:690-694,1982. During these studies, but not reported in
the article, we used the chemical indicator and spore strips. We found
that the chemical indicator was more conservative (i.e. the chemical
indicator showed unsafe conditions while the spore strips showed complete
kill (no growth)). I believe that this indicator is now called SteriGage
and can be obtained At PyMaH Corp. 89 Route 206, P.O. Box 1114,
Somerville, New Jersey 08876. One nice thing about the chemical indicator
is that one can tape it to a stick and than place it into the waste
material. After the cycle, you can have a reading before you dispose of
the waste.
Jim Lauer
University of Minnesota
Environmental Health & Safety
W-158
626-5621
lauer001@maroon.tc.umn.edu
=========================================================================
Date: Mon, 20 Dec 1999 16:50:20 +0100
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Doblhoff-dier Otto
Organization: Universitaet fuer Bodenkultur Wien
Subject: Re: UNIDO/BATS Biosafety Workshop
In-Reply-To:
MIME-Version: 1.0
Content-type: text/plain; charset=ISO-8859-1
Content-transfer-encoding: Quoted-printable
Dear Diane,
all the best for 2000 and off-course the book and happy merry xmas
Otto
Otto Doblhoff-Dier, Inst. Appl. Microbiol, Univ. Agric.,
Nussdorfer L=E4nde 11, A-1190 Vienna, Austria, Europe
Tel: *43-1-36006-6204 Fax:*43-1-3697615
EMAIL: doblhoff@edv2.boku.ac.at
WWW:
=========================================================================
Date: Mon, 20 Dec 1999 11:44:03 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: kathy joseph
Subject: Re: Autoclave validation
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"; format=flowed
3M acquired the indicators mentioned here and did some retesting
before they came back out as Comply Chemical Integrators. The
pamphlet I have shows a picture with Comply featured prominently
and SteriGage less so. The item # appears to be 1243 and the
phone number is 800-228-3957.
Kathy
>For many years we used a chemical indicator, called Thermolog S, that
>integrated time, temperature and moisture conditions to validate the
>decontamanation of laboratory (culture media) waste in a gravity type
>autoclave operating at 121 degrees C. ....
>kill (no growth)). I believe that this indicator is now called SteriGage
>and can be obtained At PyMaH Corp. 89 Route 206, P.O. Box 1114,
>Somerville, New Jersey 08876. One nice thing about the chemical indicator
>is that one can tape it to a stick and than place it into the waste
>material. After the cycle, you can have a reading before you dispose of
>the waste.
>
>Jim Lauer
>University of Minnesota
>Environmental Health & Safety
>W-158
>626-5621
>lauer001@maroon.tc.umn.edu
Kathy Joseph
Associate Biosafety Officer
Harvard University
617 432-2841 fax 617 432-4730
uos.harvard.edu/ehs
=========================================================================
Date: Mon, 20 Dec 1999 17:03:41 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Johnson, Julie A."
Subject: tests procedures for HIV and HBV in human cell lines
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
I have a researcher who would like to have a Human Embryonic Kidney (HEK)
cell line tested for HIV and HBV before they begin using them. Can anyone
recommend specific appropriate protocols for testing for these viruses?
Julie A. Johnson, Ph.D.
Biosafety Officer
Environmental Health and Safety
Iowa State University
Ames, IA 50011
e-mail: jajohns@iastate.edu
phone: 515-294-7657
fax: 515-294-9357
web site:
=========================================================================
Date: Tue, 21 Dec 1999 09:12:41 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Ed Krisiunas
Subject: Glutaraldehyde
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Happy Holidays to all!
Is there a new ceiling level for glutaraldehyde (previously 0.2 ppm)? I was
told it is 0.05 ppm. If so, can anyone provide a reference?
Regards,
Ed Krisiunas, MT(ASCP), CIC, MPH
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
=========================================================================
Date: Tue, 21 Dec 1999 09:27:15 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Bernholc, Nicole M"
Subject: Re: Glutaraldehyde
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
ACGIH TLV ceiling adopted in 1999 is 00.05 tHE NOTATION IS SEN (sensitizer)
AND a4 (not classified as a human carcinogen).
-----Original Message-----
From: Ed Krisiunas [mailto:EKrisiunas@]
Sent: Tuesday, December 21, 1999 9:13 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: Glutaraldehyde
Happy Holidays to all!
Is there a new ceiling level for glutaraldehyde (previously 0.2 ppm)? I was
told it is 0.05 ppm. If so, can anyone provide a reference?
Regards,
Ed Krisiunas, MT(ASCP), CIC, MPH
INSCITE
115 Lyons Road
Burlington, Connecticut
06013
860-675-1217
860-675-1311(fax)
=========================================================================
Date: Tue, 21 Dec 1999 09:03:16 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Petty, Carol"
Subject: Re: Glutaraldehyde
MIME-Version: 1.0
Content-Type: text/plain
According to the 1999 TLV's and BEIs, the action of .05 is correct.
Carol L. Petty, C.I.H.
Industrial Hygienist
Phone: (505) 845-1076
Fax: (505) 845-1174
email: cpetty@
> -----Original Message-----
> From: Ed Krisiunas [SMTP:EKrisiunas@]
> Sent: Tuesday, December 21, 1999 7:13 AM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: Glutaraldehyde
>
> Happy Holidays to all!
>
> Is there a new ceiling level for glutaraldehyde (previously 0.2 ppm)? I
> was
> told it is 0.05 ppm. If so, can anyone provide a reference?
>
> Regards,
>
> Ed Krisiunas, MT(ASCP), CIC, MPH
> INSCITE
> 115 Lyons Road
> Burlington, Connecticut
> 06013
> 860-675-1217
> 860-675-1311(fax)
=========================================================================
Date: Tue, 21 Dec 1999 19:29:45 EST
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Jim Kaufman
Subject: Lab Worker Suffers UV Burns
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
Subj: [Safe-NZ] accident warning
Date: 99-12-20 20:23:26 EST
From: n.mccarter@.nz (Nigel McCarter)
Sender: safe-nz-admin@niwa.cri.nz
To: Safe-NZ@niwa.cri.nz
There has been a serious harm accident at one NZ CRI where a technician
suffered uv burns whilst working with a laminar flow cupboard.
This particular cabinet is an older design and there were no interlocked
perspex doors. As a result the UV light could be propagated outside of the
cabinet though this was not normally a problem as exposure times were
usually short.
However, in this case, the UV was inadvertently left on during a overnight
experiment exposing one person.
The technician suffered skin and retinal burns but is fully recovered.
This is the second laminar flow cabinet accident to be reported from a
science institute in the last two months, (the last was from the UK).
I also note at some other NZ sites during safety audits that some older
cabinets have been refurbished with higher wattage UV lamps.
However, for all science labs that have not received a recent safety audit,
I recommend that lab managers immediately check all laminar flow cupboards
and ensure that:
(a) appropriate UV absorbent plastic screens are in place.
(b) the UV lights are interlocked with the screen so that the UV light
cannot be left on with the screen open.
(c) Post appropriate warning signs on the front of the cabinet.
(e) update laboratory manuals.
(d) ensure lab staff are aware of the UV hazard.
If you would like further information please contact me directly.
Nigel McCarter
Safety Management and Information Services Ltd
Box 23 019 Hamilton
Phone 64 7 858 2429 Fax 64 858 2689
Mobile 02 212 4901
=========================================================================
Date: Wed, 22 Dec 1999 00:25:27 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Thomas J Shelley
Subject: Introduction and UV Lights
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/enriched; charset="us-ascii"
Dear Colleagues--I am a recent subscriber to the BIOSAFTY list. I am
the
Chemical Hygiene Officer at Cornell University. I don't become too
involved
with biosafety issues, although I work closely with our BSO on various
laboratory issues of mutual interest. Greetings to my friends and
acquaintances who are subscribers to this list.
WARNING: Soapbox being mounted.
Nigel McCarter wrote in part:
>There has been a serious harm accident at one NZ CRI where a
technician
>suffered uv burns whilst working with a laminar flow cupboard.
UV lights in laminar flow clean benches and BSCs are one of my pet
peeves. For many years, prior to handing this duty over to our BSO,
I was charged with the prior approval of the purchase of these
devices. We had a UV burn incident at Cornell several years ago,
after which I had an extensive conversation with a representative of
the Baker Company (the BSC manufacturer, not the chemical company)
concerning UV lights. During this conversation I was told the
following:
1) A flux of 25 microwatts per square centimeter is required for UV
to be an effective biocidal agent.
2) The maximum life of the average UV light is about six (6) months.
After this most UV lights fail to provide the required 25 microwatts
per square centimeter. Many fail before six months.
3) Even 10% of the new lamps purchased by Baker for
their BSCs do not
meet the above requirement and are discarded.
4) If you are depending upon a UV light as a biocide you must
routinely
use a UV flux meter to determine that your UV light maintains the
required UV flux of 25 microwatts per square centimeter and be
prepared to replace the UV light as required.
5) The potential for damaging UV exposure exists long after the flux
of UV light falls below the effective biocidal level.
6) Baker tries to dissuade its customers from purchasing UV lights
for BSCs and clean benches.
My take on the above is the following:
1) 90% or more of the UV lights in use as biocidal agents
aren't
as they are older than 6 months and have failed to provide
the
required UV flux for a long time.
2) The average researcher has never even heard of a UV flux
meter, let alone owns such a device or knows how to use it
or how to keep it in calibration.
3) Chemical disinfecting agents used in an appropriate program
are far superior to UV lights.
4) The potential for serious eye and skin injury is not worth the
risk
associated with the use of UV lights.
I actively dissuade researchers from purchasing UV lights for BSCs
and laminar flow clean benches. Old cultural ways die hard, however,
and many researchers buy the UV lights anyway. Even though they know
that they only last a few months and are expensive to replace and
create an unwarranted occupational hazard.
Dismounting soapbox..... Have a great Holiday. Tom Shelley
"There are old scientists and there are bold scientists, but there
are no old, bold scientists." Anon.
*****************************************************
Tom Shelley, Chemical Hygiene Officer, Cornell University
Department of Environmental Health and Safety, 125 Humphreys Service
Building
Ithaca, NY 14853. (607) 255-4288 tjs1@cornell.edu
****************************DISCLAIMER*****************
The comments and views expressed in this communication are strictly my
own and
are not to be construed to officially represent those of my peers,
supervisors
or Cornell University.
=========================================================================
Date: Wed, 22 Dec 1999 09:24:20 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "J.H. Keene"
Subject: Re: Introduction and UV Lights
MIME-Version: 1.0
Content-Type: multipart/alternative;
boundary="----=_NextPart_000_000D_01BF4C5E.53E64560"
This is a multi-part message in MIME format.
------=_NextPart_000_000D_01BF4C5E.53E64560
Content-Type: text/plain;
charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
Well said Tom. I too have been trying to disuade people from using the =
UV lights in the BSC's. The usual response I get from the researcher, =
usually someone who is doing tissue culture, is that they "need" the =
lights to ensure sterility for their tissue cultures. In going around =
the country teaching lab safety courses, I find that, in spite of the =
good training materials available on the care and use of BSC's, the vast =
majority of people using BSC's don't know how they work and therefore =
are generally overcoming the protective engineering. They think, =
however, that the UV lights, if they have them, will protect both them =
and their work. Somehow we Biosafety Professionals have to get the =
point across that BSC's are safety devices and lab supervisors and =
personnel must be informed of how they work and required to work =
appropriately in them.
John H. Keene, Dr. P.H., CBSP(ABSA)
Biohaztec Associates, Inc.
Midlothian, Va 23113
----- Original Message -----=20
From: Thomas J Shelley=20
To: BIOSAFTY@MITVMA.MIT.EDU=20
Sent: Wednesday, December 22, 1999 12:25 AM
Subject: Introduction and UV Lights
Dear Colleagues--I am a recent subscriber to the BIOSAFTY list. I am =
the
Chemical Hygiene Officer at Cornell University. I don't become too =
involved
with biosafety issues, although I work closely with our BSO on various
laboratory issues of mutual interest. Greetings to my friends and
acquaintances who are subscribers to this list.
WARNING: Soapbox being mounted.
Nigel McCarter wrote in part:
>There has been a serious harm accident at one NZ CRI where a =
technician
>suffered uv burns whilst working with a laminar flow cupboard.
UV lights in laminar flow clean benches and BSCs are one of my pet
peeves. For many years, prior to handing this duty over to our BSO,
I was charged with the prior approval of the purchase of these
devices. We had a UV burn incident at Cornell several years ago,
after which I had an extensive conversation with a representative of
the Baker Company (the BSC manufacturer, not the chemical company)
concerning UV lights. During this conversation I was told the =
following:
1) A flux of 25 microwatts per square centimeter is required for UV
to be an effective biocidal agent.
2) The maximum life of the average UV light is about six (6) months.
After this most UV lights fail to provide the required 25 microwatts
per square centimeter. Many fail before six months.
3) Even 10% of the new lamps purchased by Baker for their BSCs do not
meet the above requirement and are discarded.
4) If you are depending upon a UV light as a biocide you must =
routinely
use a UV flux meter to determine that your UV light maintains the
required UV flux of 25 microwatts per square centimeter and be
prepared to replace the UV light as required.
5) The potential for damaging UV exposure exists long after the flux
of UV light falls below the effective biocidal level.
6) Baker tries to dissuade its customers from purchasing UV lights
for BSCs and clean benches.
My take on the above is the following:
1) 90% or more of the UV lights in use as biocidal agents aren't
as they are older than 6 months and have failed to provide the
required UV flux for a long time.
2) The average researcher has never even heard of a UV flux
meter, let alone owns such a device or knows how to use it
or how to keep it in calibration.
3) Chemical disinfecting agents used in an appropriate program
are far superior to UV lights.
4) The potential for serious eye and skin injury is not worth the risk
associated with the use of UV lights.
I actively dissuade researchers from purchasing UV lights for BSCs
and laminar flow clean benches. Old cultural ways die hard, however,
and many researchers buy the UV lights anyway. Even though they know
that they only last a few months and are expensive to replace and
create an unwarranted occupational hazard.
Dismounting soapbox..... Have a great Holiday. Tom Shelley
"There are old scientists and there are bold scientists, but there
are no old, bold scientists." Anon.
*****************************************************
Tom Shelley, Chemical Hygiene Officer, Cornell University
Department of Environmental Health and Safety, 125 Humphreys Service =
Building
Ithaca, NY 14853. (607) 255-4288 tjs1@cornell.edu
****************************DISCLAIMER*****************
The comments and views expressed in this communication are strictly my =
own and
are not to be construed to officially represent those of my peers, =
supervisors
or Cornell University.
------=_NextPart_000_000D_01BF4C5E.53E64560
Content-Type: text/html;
charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
Well said Tom. I too have been trying to =
disuade people=20
from using the UV lights in the BSC's. The usual response I get =
from the=20
researcher, usually someone who is doing tissue culture, is that they =
"need" the=20
lights to ensure sterility for their tissue cultures. In going =
around the=20
country teaching lab safety courses, I find that, in spite of the good =
training=20
materials available on the care and use of BSC's, the vast majority =
of=20
people using BSC's don't know how they work and therefore are generally=20
overcoming the protective engineering. They think, however, that =
the UV=20
lights, if they have them, will protect both them and their work. =
Somehow=20
we Biosafety Professionals have to get the point across that BSC's are =
safety=20
devices and lab supervisors and personnel must be informed of how =
they work=20
and required to work appropriately in them.
John H. Keene, Dr. P.H., CBSP(ABSA)
Biohaztec Associates, Inc.
Midlothian, Va 23113
----- Original Message -----
From:=20
Thomas J =
Shelley=20
To: BIOSAFTY@MITVMA.MIT.EDU
Sent: Wednesday, December 22, =
1999 12:25=20
AM
Subject: Introduction and UV =
Lights
Dear Colleagues--I am a recent subscriber to the =
BIOSAFTY list.=20
I am theChemical Hygiene Officer at Cornell University. I don't =
become too=20
involvedwith biosafety issues, although I work closely with our =
BSO on=20
variouslaboratory issues of mutual interest. Greetings to my =
friends=20
andacquaintances who are subscribers to this list.WARNING: =
Soapbox=20
being mounted.Nigel McCarter wrote in part:>There =
has been=20
a serious harm accident at one NZ CRI where a =
technician>suffered uv=20
burns whilst working with a laminar flow cupboard.UV lights in =
laminar=20
flow clean benches and BSCs are one of my petpeeves. For many =
years, prior=20
to handing this duty over to our BSO,I was charged with the prior =
approval=20
of the purchase of thesedevices. We had a UV burn incident at =
Cornell=20
several years ago,after which I had an extensive conversation with =
a=20
representative ofthe Baker Company (the BSC manufacturer, not the =
chemical=20
company)concerning UV lights. During this conversation I was told =
the=20
following:1) A flux of 25 microwatts per square centimeter is =
required=20
for UVto be an effective biocidal agent.2) The maximum life of =
the=20
average UV light is about six (6) months.After this most UV lights =
fail to=20
provide the required 25 microwattsper square centimeter. Many fail =
before=20
six months.3) Even 10% of the new lamps purchased by Baker =
for=20
their BSCs do notmeet the above requirement and are =
discarded.4) If=20
you are depending upon a UV light as a biocide you must =
routinelyuse a UV=20
flux meter to determine that your UV light maintains therequired =
UV flux=20
of 25 microwatts per square centimeter and beprepared to replace =
the UV=20
light as required.5) The potential for damaging UV exposure exists =
long=20
after the fluxof UV light falls below the effective biocidal =
level.6)=20
Baker tries to dissuade its customers from purchasing UV lightsfor =
BSCs=20
and clean benches.My take on the above is the =
following:1) 90%=20
or more of the UV lights in use as biocidal agents aren'tas =
they=20
are older than 6 months and have failed to provide therequired UV =
flux for=20
a long time.2) The average researcher has never even heard of a UV =
fluxmeter, let alone owns such a device or knows how to use =
itor how=20
to keep it in calibration.3) Chemical disinfecting agents used in =
an=20
appropriate programare far superior to UV lights.4) The =
potential for=20
serious eye and skin injury is not worth the riskassociated with =
the use=20
of UV lights.I actively dissuade researchers from purchasing =
UV lights=20
for BSCsand laminar flow clean benches. Old cultural ways die =
hard,=20
however,and many researchers buy the UV lights anyway. Even though =
they=20
knowthat they only last a few months and are expensive to replace=20
andcreate an unwarranted occupational hazard.Dismounting=20
soapbox..... Have a great Holiday. Tom Shelley"There are old=20
scientists and there are bold scientists, but thereare no old, =
bold=20
scientists."=20
=
Anon.*****************************************************Tom=
=20
Shelley, Chemical Hygiene Officer, Cornell UniversityDepartment of =
Environmental Health and Safety, 125 Humphreys Service =
BuildingIthaca, NY=20
14853. (607) 255-4288=20
=
tjs1@cornell.edu****************************DISCLAIMER***********=
******The=20
comments and views expressed in this communication are strictly my own =
andare not to be construed to officially represent those of my =
peers,=20
supervisorsor Cornell University.
------=_NextPart_000_000D_01BF4C5E.53E64560--
=========================================================================
Date: Wed, 22 Dec 1999 09:41:26 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Sandra Fry
Subject: BSL3 decon alternatives....
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: quoted-printable
Hello everyone...
Does anyone have experience using a product called "Lysofume" or "Profilm" =
to decontaminate a BSL3 laboratory before annual maintenance? This method =
is being promoted as an alternative to fumigation and I am having =
difficulty finding any information other than the manufacturers spec's.
Thanks!
Sandra Fry
Chief, Biohazard Containment
CFIA
59 Camelot Drive, rm 3045
Nepean, Ontario
K1A 0Y9
bus (613) 225 3242 (4255)
fax (613) 228 6656
ans (613) 755 3253
=========================================================================
Date: Wed, 22 Dec 1999 09:07:37 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Judy Pointer
Subject: Re: Introduction and UV Lights
Mime-Version: 1.0
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Boundary="0__=gBLQrnucc2vErBPEfrfl1KFKRq4D1Ksyk9Mp8IdRVqScM1cz3PjD17Vv"
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Content-type: text/plain; charset=us-ascii
Content-Disposition: inline
I agree and have been trying to find a good short video - to make required
viewing - on how to work safely in BSCs. If it covers all the points of Sec. V
of the CDC book on BSCs, I'll buy it. Does anyone have a source?
"J.H. Keene" on 12/22/99 08:24:20 AM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Judy M. Pointer/MDACC)
Subject: Re: Introduction and UV Lights
Well said Tom. I too have been trying to disuade people from using the UV
lights in the BSC's. The usual response I get from the researcher, usually
someone who is doing tissue culture, is that they "need" the lights to ensure
sterility for their tissue cultures. In going around the country teaching lab
safety courses, I find that, in spite of the good training materials available
on the care and use of BSC's, the vast majority of people using BSC's don't know
how they work and therefore are generally overcoming the protective engineering.
They think, however, that the UV lights, if they have them, will protect both
them and their work. Somehow we Biosafety Professionals have to get the point
across that BSC's are safety devices and lab supervisors and personnel must be
informed of how they work and required to work appropriately in them.
John H. Keene, Dr. P.H., CBSP(ABSA)
Biohaztec Associates, Inc.
Midlothian, Va 23113
----- Original Message -----
From: Thomas J Shelley
To: BIOSAFTY@MITVMA.MIT.EDU
Sent: Wednesday, December 22, 1999 12:25 AM
Subject: Introduction and UV Lights
Dear Colleagues--I am a recent subscriber to the BIOSAFTY list. I am the
Chemical Hygiene Officer at Cornell University. I don't become too involved
with biosafety issues, although I work closely with our BSO on various
laboratory issues of mutual interest. Greetings to my friends and
acquaintances who are subscribers to this list.
WARNING: Soapbox being mounted.
Nigel McCarter wrote in part:
>There has been a serious harm accident at one NZ CRI where a technician
>suffered uv burns whilst working with a laminar flow cupboard.
UV lights in laminar flow clean benches and BSCs are one of my pet
peeves. For many years, prior to handing this duty over to our BSO,
I was charged with the prior approval of the purchase of these
devices. We had a UV burn incident at Cornell several years ago,
after which I had an extensive conversation with a representative of
the Baker Company (the BSC manufacturer, not the chemical company)
concerning UV lights. During this conversation I was told the following:
1) A flux of 25 microwatts per square centimeter is required for UV
to be an effective biocidal agent.
2) The maximum life of the average UV light is about six (6) months.
After this most UV lights fail to provide the required 25 microwatts
per square centimeter. Many fail before six months.
3) Even 10% of the new lamps purchased by Baker for their BSCs do not
meet the above requirement and are discarded.
4) If you are depending upon a UV light as a biocide you must routinely
use a UV flux meter to determine that your UV light maintains the
required UV flux of 25 microwatts per square centimeter and be
prepared to replace the UV light as required.
5) The potential for damaging UV exposure exists long after the flux
of UV light falls below the effective biocidal level.
6) Baker tries to dissuade its customers from purchasing UV lights
for BSCs and clean benches.
My take on the above is the following:
1) 90% or more of the UV lights in use as biocidal agents aren't
as they are older than 6 months and have failed to provide the
required UV flux for a long time.
2) The average researcher has never even heard of a UV flux
meter, let alone owns such a device or knows how to use it
or how to keep it in calibration.
3) Chemical disinfecting agents used in an appropriate program
are far superior to UV lights.
4) The potential for serious eye and skin injury is not worth the risk
associated with the use of UV lights.
I actively dissuade researchers from purchasing UV lights for BSCs
and laminar flow clean benches. Old cultural ways die hard, however,
and many researchers buy the UV lights anyway. Even though they know
that they only last a few months and are expensive to replace and
create an unwarranted occupational hazard.
Dismounting soapbox..... Have a great Holiday. Tom Shelley
"There are old scientists and there are bold scientists, but there
are no old, bold scientists." Anon.
*****************************************************
Tom Shelley, Chemical Hygiene Officer, Cornell University
Department of Environmental Health and Safety, 125 Humphreys Service Building
Ithaca, NY 14853. (607) 255-4288 tjs1@cornell.edu
****************************DISCLAIMER*****************
The comments and views expressed in this communication are strictly my own and
are not to be construed to officially represent those of my peers, supervisors
or Cornell University.
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=========================================================================
Date: Wed, 22 Dec 1999 10:15:24 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Elizabeth Smith
Subject: Re: Autoclave validation
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
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Our Validation Department uses ampoules of B. stearothermopholus (spelling?)
for validating autoclaves. This is the critical point of demonstrating that
the autoclave is actually achieving the temperature AND pressure indicated
on it's various gauges/read-outs.
We utilize ThermoLogs for each load during the operation of the autoclaves.
These little gizmos indicate colorimetrically that both appropriate pressure
and temperature have been achieved.
The Validation crew prefers the traditional biological indicators as a more
reliable and becuase it is a validated test method (we're looking not only
at biological safety, but cGMP for product quality).
Elizabeth E. Smith
Environmental, Health & Safety Manager
BioPort Corporation
3500 N. Martin L. King Blvd.
Lansing, Michigan 48906
517-327-6806
There is nothing to learn from history but that men do not learn from
history. - Hegel
__________________________________________________
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=========================================================================
Date: Wed, 22 Dec 1999 10:39:40 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: Introduction and UV Lights
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
At 09:07 AM 12/22/99 -0600, you wrote:
>I agree and have been trying to find a good short video - to make required
>viewing - on how to work safely in BSCs. If it covers all the points of Sec.
V
>of the CDC book on BSCs, I'll buy it. Does anyone have a source?
>
The only video I know of is: Safe Use of Biological Safety Cabinets or The
Case
of the Contaminated Cultures from Eagleson Institute. It gets a bit silly at
times but gives a good basic background.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Wed, 22 Dec 1999 10:41:27 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Richard Fink
Subject: Re: BSL3 decon alternatives....
In-Reply-To:
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
At 09:41 AM 12/22/99 -0500, you wrote:
>Hello everyone...
>Does anyone have experience using a product called "Lysofume" or "Profilm" to
decontaminate a BSL3 laboratory before annual maintenance? This method is
being
promoted as an alternative to fumigation and I am having difficulty finding
any
information other than the manufacturers spec's.
>
>Thanks!
>
>Sandra Fry
Do you have any info regarding ingredients in these compounds. You want
something that is at a minimum a high level disinfectant, preferably a cold
chemical sterilant. We still use paraformaldehyde--->formaldehyde.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Wed, 22 Dec 1999 11:16:32 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Mike Wotring
Organization: University of Scranton
Subject: BSC training
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I found some free videos on the Howard Hughes Medical Institute's web
site for lab safety. I have ordered them but not received them yet, so
I can't vouch for their efficacy, but they are free! One of them deals
specifically with mammalian cell culture hazards, so it probably covers
BSC use to some extent. The address is:
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n:Wotring;Michael
tel;fax:570-941-6229
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=========================================================================
Date: Wed, 22 Dec 1999 08:36:38 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: UV Lights - NOT!
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Morning, All -
How interesting to find this thread first thing this morning. I was
thinking about this very issue while coming in to the office and I
wholeheartedly agree with all that's been said. Far too many lab scientists
consider the biosafety cabinet UV lamp a panacea that allows them to blow
off good aseptic procedure and decon practices. And these folks are well
aware that the "killing range" of UV is fairly short, the UV dose that
reaches the work surface is often inadequate to do anything, the lamps are
expensive to replace and they offer significant risks if misused. Yet they
routinely not only use them but rely on them to "keep things clean".
The output performance of UV lamps is also severely degraded by the buildup
of dust that occurs on the charged lamp surface. I usually tell people
that, while the lamps are probably worthless and no substitute for good
technique, their output can be maximized by gently wiping the cold lamp
surface with a Kimwipe moistened with 70% ethanol at least weekly. But I'm
reluctant to offer this advice for fear someone will actually break the lamp
in situ doing this.
The comment about "old ways dying hard" is certainly true here. Trying to
convince someone who has relied on the UV for years that it really isn't
either necessary or a good idea is an exercise in frustration. It's like
trying to convince the same guy he really doesn't need and shouldn't use the
bunsen burner in the hood - you know how this conversation goes:
"Nonsense - I've always done it that way. I have to flame my pipettes and
bottle lips."
"Why? Theyre plastic and sterile-packaged."
"Just because I do - I always have. Otherwise, I'll get contamination."
"Do you get any contamination now?"
"Well, yeah, on rare occasion ..."
"Allow me to suggest that your "rare" contamination problems may go away if
you stop flaming."
"Nonsense - I've always done it this way and I've always had some
contamination, just like everyone else."
"OK - next time we have a hood fire, I'll come and get you ..."
Someday, when I have the luxury of some spare time, I'm going to develop a
session on the design, care and feeding of biosafety cabinets for all
research staff. I figure if I can help them understand how the things work
and how easy it is to undermine the operational parameters by using what
many consider "standard procedures", perhaps I can make some headway in
breaking those "old ways" that die so hard. Meanwhile, I second Judy's
question - if any of you know of a good hood use video, please tell me. I
did not adopt the Eagleson video because I thought it was too loose and
simplistic, even though it made some good points. The Howard Hughes videos
are outstanding but the only way to get a hood-specific video would be to
create one with outtakes from several of their other videos.
Enough rambling. Welcome, Tom Shelley! And to all, a very happy Holiday
and a totally cool New Year.
-- Glenn
------------------------------------------------------
Glenn A. Funk, Ph.D., CBSP
Biosafety Officer
University of California, San Francisco
Voice 415-476-2097
Fax 415-476-0581
glennf@ehsmail.ucsf.edu
=========================================================================
Date: Wed, 22 Dec 1999 11:36:30 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Don Callihan
Subject: Re: Introduction and UV Lights
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From: Don Callihan@BDX on 12/22/99 11:36 AM
I wholeheartedly agree with the issues that Tom has raised. In my opinion, this
is something that should be addressed as soon as possible, both to prevent UV
exposure injuries such as those that have been discussed and to get a firm
position on this subject into the biosafety literature and appropriate
"standards" such as BMBL, NSF49 (if appropriate), et al.
Two questions:
Is there an appropriate ABSA Committee that should be asked to work on this
issue?
Is there any information on the number of UV related work injuries in the past
few years? (Perhaps our "off line" e-mail system could be used to gather such
data.)
This issue has been out there as long as I can remember and someone (regulatory
or otherwise) needs to take the lead on either banning the installation and use
of UV lights in BSCs or a widespread education effort on the risks/benefits of
their use, plus appropriate training and maintenance guidelines.
Just my personal opinion.
Don Callihan
Biosafety Officer
BD Biosciences
Sparks, MD 22152
410.773.6684
Thomas J Shelley on 12/22/99 12:25:27 AM
Please respond to A Biosafety Discussion List
To: BIOSAFTY@MITVMA.MIT.EDU
cc: (bcc: Don Callihan/BALT/BDX)
Subject: Introduction and UV Lights
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Dear Colleagues--I am a recent subscriber to the BIOSAFTY list. I am the
Chemical Hygiene Officer at Cornell University. I don't become too involved
with biosafety issues, although I work closely with our BSO on various
laboratory issues of mutual interest. Greetings to my friends and
acquaintances who are subscribers to this list.
WARNING: Soapbox being mounted.
Nigel McCarter wrote in part:
>There has been a serious harm accident at one NZ CRI where a technician
>suffered uv burns whilst working with a laminar flow cupboard.
UV lights in laminar flow clean benches and BSCs are one of my pet
peeves. For many years, prior to handing this duty over to our BSO,
I was charged with the prior approval of the purchase of these
devices. We had a UV burn incident at Cornell several years ago,
after which I had an extensive conversation with a representative of
the Baker Company (the BSC manufacturer, not the chemical company)
concerning UV lights. During this conversation I was told the following:
1) A flux of 25 microwatts per square centimeter is required for UV
to be an effective biocidal agent.
2) The maximum life of the average UV light is about six (6) months.
After this most UV lights fail to provide the required 25 microwatts
per square centimeter. Many fail before six months.
3) Even 10% of the new lamps purchased by Baker for their BSCs do not
meet the above requirement and are discarded.
4) If you are depending upon a UV light as a biocide you must routinely
use a UV flux meter to determine that your UV light maintains the
required UV flux of 25 microwatts per square centimeter and be
prepared to replace the UV light as required.
5) The potential for damaging UV exposure exists long after the flux
of UV light falls below the effective biocidal level.
6) Baker tries to dissuade its customers from purchasing UV lights
for BSCs and clean benches.
My take on the above is the following:
1) 90% or more of the UV lights in use as biocidal agents aren't
as they are older than 6 months and have failed to provide the
required UV flux for a long time.
2) The average researcher has never even heard of a UV flux
meter, let alone owns such a device or knows how to use it
or how to keep it in calibration.
3) Chemical disinfecting agents used in an appropriate program
are far superior to UV lights.
4) The potential for serious eye and skin injury is not worth the risk
associated with the use of UV lights.
I actively dissuade researchers from purchasing UV lights for BSCs
and laminar flow clean benches. Old cultural ways die hard, however,
and many researchers buy the UV lights anyway. Even though they know
that they only last a few months and are expensive to replace and
create an unwarranted occupational hazard.
Dismounting soapbox..... Have a great Holiday. Tom Shelley
"There are old scientists and there are bold scientists, but there
are no old, bold scientists." Anon.
*****************************************************
Tom Shelley, Chemical Hygiene Officer, Cornell University
Department of Environmental Health and Safety, 125 Humphreys Service Building
Ithaca, NY 14853. (607) 255-4288 tjs1@cornell.edu
****************************DISCLAIMER*****************
The comments and views expressed in this communication are strictly my own and
are not to be construed to officially represent those of my peers, supervisors
or Cornell University.
--0__=T5Rn8X73h7EdlnXvfz6rYFXqKhuCQBRAqmwgqDAyA5TLBlaqFX5r84qA--
=========================================================================
Date: Wed, 22 Dec 1999 16:43:41 -0000
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stuart Thompson
Subject: Re: UV Lights - NOT!
In-Reply-To:
MIME-Version: 1.0
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What do list members think about UV lights in PCR cabinets? These are
claimed to inactivate stray nucleic acids so that your amplified nucleic
acid is really that which was placed in the sample vial at the scene of the
crime and did not arise from a piece of dandruff shed by the previous user
of the cabinet.
> -----Original Message-----
> From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
> Behalf Of Funk, Glenn
> Sent: Wednesday, December 22, 1999 4:37 PM
> To: BIOSAFTY@MITVMA.MIT.EDU
> Subject: UV Lights - NOT!
=========================================================================
Date: Wed, 22 Dec 1999 11:42:11 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Sandra Fry
Subject: Re: BSL3 decon alternatives.... -Reply
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This is an interesting product....it is mixed with cold water and sprayed =
on with an applicator, 1 gallon will cover approx 5,000 square feet, and =
it releases 6-8% formaldehyde under a "film". It also remains active for =
at least 7 days or until removed. It also contains 2 Hydroxymethyl-2- =
nitro-3- proanediol. Upjohn lists the product as a "fumigant disinfectant =
solution" for "livestock and poultry premises". One of my BSL3 concerns =
however, is that it is a surface disinfectant, and is not gaseous.=20
>>> Richard Fink 12/22 10:41 am >>>
At 09:41 AM 12/22/99 -0500, you wrote:
>Hello everyone...
>Does anyone have experience using a product called "Lysofume" or =
"Profilm" to
decontaminate a BSL3 laboratory before annual maintenance? This method is
being
promoted as an alternative to fumigation and I am having difficulty =
finding
any
information other than the manufacturers spec's.
>
>Thanks!
>
>Sandra Fry
Do you have any info regarding ingredients in these compounds. You want
something that is at a minimum a high level disinfectant, preferably a =
cold
chemical sterilant. We still use paraformaldehyde--->formaldehyde.
Richard Fink, SM(NRM), CBSP
Assoc. Biosafety Officer
Mass. Inst. of Tech.
617-258-5647
rfink@mit.edu
=========================================================================
Date: Wed, 22 Dec 1999 11:36:45 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Joseph H. Coggin Jr."
Subject: Re: BSC training
In-Reply-To:
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
The BMBL 4th and previous editions have an excellent section on Biosafety
Cabinets {Appendix A, pp. 200-211 adn how they work and how to clean them.
Joe Coggin
=========================================================================
Date: Wed, 22 Dec 1999 13:49:50 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Greg Merkle
Organization: Wright State University
Subject: Re: BSL3 decon alternatives.... -Reply
MIME-version: 1.0
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Sandra,
Is the product available through Upjohn or another vendor?
What does the MSDS for the product list as being exposure
hazards, etc? I would also like to see product information
on the intended uses for the product. If there has not been
any study trials on the product as a BSL3 decontaminant I
would question its usefulness in a BSL3 facility.
Now, I have a question about a product that I have heard can
be used to decontaminte BSC filters when they are being
removed. There was a service person who removed an exhaust
filter from a BSC in a laboratory that works with HIV and
other BSL2 materials (i.e.; human blood) without
decontaminating the BSC by fumigation with
paraformaldehyde. Is there such a product that can be used
for removing filters without fumigation? I have only heard
part of the story about the service work but I am concerned
about the potentials of somebody doing work that could
expose others to possible harm. I just heard about all of
this late yesterday (12/21) and I have not been able to
verify much of what I have heard. If there is a product for
this sort of use what is it and what are its directions for
safe use.
Greg Merkle
Senior Industrial Hygienist
Sandra Fry wrote:
>
> This is an interesting product....it is mixed with cold water and sprayed on with an applicator, 1 gallon will cover approx 5,000 square feet, and it releases 6-8% formaldehyde under a "film". It also remains active for at least 7 days or until removed. It also contains 2 Hydroxymethyl-2- nitro-3- proanediol. Upjohn lists the product as a "fumigant disinfectant solution" for "livestock and poultry premises". One of my BSL3 concerns however, is that it is a surface disinfectant, and is not gaseous.
>
> >>> Richard Fink 12/22 10:41 am >>>
> At 09:41 AM 12/22/99 -0500, you wrote:
> >Hello everyone...
> >Does anyone have experience using a product called "Lysofume" or "Profilm" to
> decontaminate a BSL3 laboratory before annual maintenance? This method is
> being
> promoted as an alternative to fumigation and I am having difficulty finding
> any
> information other than the manufacturers spec's.
> >
> >Thanks!
> >
> >Sandra Fry
> Do you have any info regarding ingredients in these compounds. You want
> something that is at a minimum a high level disinfectant, preferably a cold
> chemical sterilant. We still use paraformaldehyde--->formaldehyde.
>
> Richard Fink, SM(NRM), CBSP
> Assoc. Biosafety Officer
> Mass. Inst. of Tech.
> 617-258-5647
> rfink@mit.edu
=========================================================================
Date: Wed, 22 Dec 1999 12:24:37 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: bunsen burner
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
this is a benchtop burner. It is a new one because the old one was thought
to have been leaky. Lingering odor of natural gas after use. the lab has
attached to the gas turret with clear tubing (perhaps Tygon brand) Burner
is from Teledyne. I am trying to figure out the appropriate type of tubing.
any ideas?
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
4200 E. 9th Ave.
Denver, CO 80262
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
=========================================================================
Date: Wed, 22 Dec 1999 15:59:36 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Cheri Marcham
Subject: pichia pastoris
MIME-Version: 1.0
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I have my first request from a researcher for expressing a protein in a
Pichia pastoris expression system. I am going to assume, based on the
limited information I have from the vendor of such a system, that it is
probably similar to using E. coli, with certain advantages, but my question
is, under what classification in the NIH Guidelines does this fall? I
assume the organism is a risk group 1, since it is not listed in 2 or above,
and I cannot find an exemption for such a system, or am I not looking in the
right place?
Cheri Marcham
cheri-marcham@ouhsc.edu
The University of Oklahoma Health Sciences Center
=========================================================================
Date: Thu, 23 Dec 1999 09:34:29 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Stefan Wagener
Subject: Re: pichia pastoris
In-Reply-To:
MIME-Version: 1.0
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The P. pastoris expression system is a very effective system to generate
high levels of recombinant protein. A nice overview can be found here:
You are correct in your assumption that is similar to E. coli with some
advantages. However, don't assume it is a Risk Group 1 agent only because it
is not listed under 2 or higher. The tables are not all inclusive. However,
it is my understanding that P. pastoris is not known to cause human
infection or being a pathogen for healthy human adults. "Somebody please
correct me if not true"!
To determine the correct NIH category you might want to not only look at the
expression system, in this case P. pastoris, but also at the protein and its
characteristics/function. Right now, the NIH guidelines exempt the
Saccaromyces system, but not P. pastoris (yet). Unless the project meets one
or more of the definitions in Section III-F, it should be considered III-E,
again depending on the expression product (e.g., toxic protein).
Hope this helps.
Stefan :-) Happy holidays and Merry Christmas!!
Stefan Wagener, Ph.D, CBSP
Michigan State University, ORCBS
C-126 Research Complex Engineering
East Lansing, MI 48824
Phone: (517) 355-6503
Fax: (517) 353-4871
-----Original Message-----
From: A Biosafety Discussion List [mailto:BIOSAFTY@MITVMA.MIT.EDU]On
Behalf Of Cheri Marcham
Sent: Wednesday, December 22, 1999 5:00 PM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: pichia pastoris
I have my first request from a researcher for expressing a protein in a
Pichia pastoris expression system. I am going to assume, based on the
limited information I have from the vendor of such a system, that it is
probably similar to using E. coli, with certain advantages, but my question
is, under what classification in the NIH Guidelines does this fall? I
assume the organism is a risk group 1, since it is not listed in 2 or above,
and I cannot find an exemption for such a system, or am I not looking in the
right place?
Cheri Marcham
cheri-marcham@ouhsc.edu
The University of Oklahoma Health Sciences Center
=========================================================================
Date: Thu, 23 Dec 1999 12:07:29 -0400
Reply-To: Harriet Izenberg
Sender: A Biosafety Discussion List
From: Harriet Izenberg
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Subject: Time:
12:05 PM
12/23/99
Date:
unsubscribe
Harriet Izenberg, RBP
Institutional Biosafety Officer
Environmental Health and Radiation Safety
University of Pennsylvania
215-898-4453 (PHONE)
215-898-0140 (FAX)
e-mail: harriet@ehrs.upenn.edu
=========================================================================
Date: Mon, 27 Dec 1999 12:07:58 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Greg Merkle
Organization: Wright State University
Subject: Re: UV Lights - NOT!
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I would like to suggest that Howard Hughes Medical Institute
be approached with the idea of putting together a video
dealing with the safety issue of the UV lamps. If, as it
has been stated, there are component parts of the HHMI
existing videoes that could be incorporated into such a
project the job is half done. If HHMI does not or can not
do it and the resource material is available maybe someone
else, with HHMI's permission, can extract and develop a
useful video. More efforts need to be made to make
laboratory personnel aware of the hazards of working with
the equipment in the laboratory. Awareness needs to be
raised of the potential hazards and the realization that
sometimes the thing that will be harmful is not necessarily
biological, chemical or radioligical but a physical agent.
I am concerned with the fact that not all of the newer BSC's
are equiped with safety interlocks to turn the UV lamp off
when the sash is opened. There is a new NuAire cabinet in a
lab that operates in this fashion. If the manufacturers are
aware of the problems of use and misuse why do they make the
cabinets without the interlocking mechanisms?
I also have concerns of researchers claiming the need to use
the UV lamps to "sterilize" their cabinet but they are also
using the BSC as a storage area despite the information
given to them otherwise. Despite training, information and
the information in operators manuals BSC's continue to be
misused and the end results are complaints of contaminined
cultures and somebody having poor technique.
Greg Merkle
Senior Industrial Hygienist
"Funk, Glenn" wrote:
>
> Morning, All -
>
> How interesting to find this thread first thing this morning. I was
> thinking about this very issue while coming in to the office and I
> wholeheartedly agree with all that's been said. Far too many lab scientists
> consider the biosafety cabinet UV lamp a panacea that allows them to blow
> off good aseptic procedure and decon practices. And these folks are well
> aware that the "killing range" of UV is fairly short, the UV dose that
> reaches the work surface is often inadequate to do anything, the lamps are
> expensive to replace and they offer significant risks if misused. Yet they
> routinely not only use them but rely on them to "keep things clean".
>
> The output performance of UV lamps is also severely degraded by the buildup
> of dust that occurs on the charged lamp surface. I usually tell people
> that, while the lamps are probably worthless and no substitute for good
> technique, their output can be maximized by gently wiping the cold lamp
> surface with a Kimwipe moistened with 70% ethanol at least weekly. But I'm
> reluctant to offer this advice for fear someone will actually break the lamp
> in situ doing this.
>
> The comment about "old ways dying hard" is certainly true here. Trying to
> convince someone who has relied on the UV for years that it really isn't
> either necessary or a good idea is an exercise in frustration. It's like
> trying to convince the same guy he really doesn't need and shouldn't use the
> bunsen burner in the hood - you know how this conversation goes:
>
> "Nonsense - I've always done it that way. I have to flame my pipettes and
> bottle lips."
>
> "Why? Theyre plastic and sterile-packaged."
>
> "Just because I do - I always have. Otherwise, I'll get contamination."
>
> "Do you get any contamination now?"
>
> "Well, yeah, on rare occasion ..."
>
> "Allow me to suggest that your "rare" contamination problems may go away if
> you stop flaming."
>
> "Nonsense - I've always done it this way and I've always had some
> contamination, just like everyone else."
>
> "OK - next time we have a hood fire, I'll come and get you ..."
>
> Someday, when I have the luxury of some spare time, I'm going to develop a
> session on the design, care and feeding of biosafety cabinets for all
> research staff. I figure if I can help them understand how the things work
> and how easy it is to undermine the operational parameters by using what
> many consider "standard procedures", perhaps I can make some headway in
> breaking those "old ways" that die so hard. Meanwhile, I second Judy's
> question - if any of you know of a good hood use video, please tell me. I
> did not adopt the Eagleson video because I thought it was too loose and
> simplistic, even though it made some good points. The Howard Hughes videos
> are outstanding but the only way to get a hood-specific video would be to
> create one with outtakes from several of their other videos.
>
> Enough rambling. Welcome, Tom Shelley! And to all, a very happy Holiday
> and a totally cool New Year.
>
> -- Glenn
>
> ------------------------------------------------------
> Glenn A. Funk, Ph.D., CBSP
> Biosafety Officer
> University of California, San Francisco
> Voice 415-476-2097
> Fax 415-476-0581
> glennf@ehsmail.ucsf.edu
>
=========================================================================
Date: Mon, 27 Dec 1999 16:21:03 -0500
Reply-To: btepper@
Sender: A Biosafety Discussion List
From: "Byron S. Tepper"
Organization: @Home Network
Subject: CONTROL OF BIOHAZARDS COURSE - 2000
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S P E C I A L A N N O U N C E M E N T
The date for the year 2000 presentation of the Control of Biohazards in
the Research Laboratory course has been established as May 1-5, 2000.
This year the Control of Biohazards course was scheduled early to afford
those individuals planning to take the NRM Specialist Microbiologist
(SM) in Biological Safety Microbiology exam on Friday, June 2, 2000 the
opportunity for a comprehensive biological safety review. Subjects in
the course include the Task List for Biological Safety Microbiology that
are tested in the NRM Specialist Microbiologist exam. The satisfactory
completion of this examination, together with the academic and
experience requirements, are prerequisites for application to the
American Biological Safety Association (ABSA) for the designation of
Certified Biological Safety Professional
As for the past few years, the course will be presented at the Omni
Hotel situated near the Baltimore Inner Harbor. Full details and
applications are in the brochure below.
Control of BIOHAZARDS
in the Research Laboratory
May 1-5, 2000
Presented by
The Biosafety Division Health,
Safety and Environment
The Center for Occupational
and Environmental Health
and
The Educational Resource Center
In Occupational Safety and Health
The Johns Hopkins University
Baltimore, Maryland 21205
Course Description
The Control of Biohazards in the Research Laboratory is designed to
provide instruction on the recognition and control of biohazards
including infectious agents, oncogenic viruses, recombinant DNA,
chemical carcinogens and other toxic agents. Instruction includes
facility design, practices and procedures of biohazard control,
biosafety regulations, and the organization, planning and implementation
of a biosafety program.
This Control of Biohazards in the Research Laboratory course provides an
excellent overview of biological safety for those individuals preparing
to take the NRM Specialist Microbiologist (SM) exam in Biological
Safety Microbiology. Subjects in the course include the Task List for
Biological Safety Microbiology that are tested in the NRM Specialist
Microbiologist exam. The satisfactory completion of this examination,
together with the academic and experience requirements, are
prerequisites for application to the American Biological Safety
Association (ABSA) for the designation of Certified Biological Safety
Professional (CBSP).
Who Should Attend:
The course is directed to biosafety officers, safety officers,
industrial hygienists, directors of health and safety programs,
biosafety committee chairs and members, occupational health personnel,
clinical and biomedical laboratory supervisors, bench scientists and
technicians, architects and facility engineers. This course also
provides the background needed for individuals applying to the American
Biological Safety Association for designations as Registered Biological
Safety Professional or Certified Biological Safety Professional. The
course is designed to provide basic information to participants with or
without prior biosafety experience.
Course Features:
This four and one half day course consists of lectures, a laboratory
exercise and opportunities for informal discussions with course faculty,
for review of pertinent audiovisual materials and for examination of
biosafety equipment and devices. Theoretical background and basic
principles will be covered.
Subject Areas:
An overview of cell biology and host-parasite relationships
Hazard potential of infectious agents, recombinant DNA and oncogenic
viruses
Dissemination of contaminants
Equipment designed for safety
Containment concepts: primary and secondary barriers
Personal practices and hygiene
Biosafety guideline sources
Universal precautions and bloodborne pathogens
Safe handling and housing of laboratory animals
Sterilization and disinfection
Emerging Diseases
Tuberculosis overview
Emergency procedures
Federal regulations involving laboratory safety
Packaging and shipment of infectious substances.
General Information
Dates:
May 1-5, 2000
Place:
Omni Inner Harbor Hotel
101 West Fayette Street
Baltimore, MD 21201
Registration:
8:00-8:30 a.m., Monday, May 1, 2000
Fee:
$1575 per person to include registration, continental breakfasts,
refreshments, 2 lunches and a banquet. All fees are payable in
advance. A letter confirming enrollment will be sent to each
registrant. Foreign payments must be made in a draft on a U.S. bank.
Only one-half of the fee will be returned if withdrawal is made after
April 14, 2000.
Course Credits:
This program qualifies for 3 Continuing Education Credits from the Johns
Hopkins University.
This course also qualifies for ABIH Certification Maintenance Points,
BCSP Continuance of Certification Points, and AAHP Continuing Education
Credits.
Course Manual:
A course manual will provide lecture outlines, data tables and graphics
used in the lectures, copies of applicable standards and regulations,
and other supporting materials. The manual comes in two volumes and
weighs about ten pounds, so plan for this on your return trip.
Social Functions:
A complimentary banquet for registrants will be held on Thursday , May
4. Guests are invited for a charge of $40.00.
Lodging:
Accommodations have been reserved at the Omni Inner Harbor Hotel. Rate:
$159 Single or Double - guaranteed until April 14, 2000.
Note:
The Johns Hopkins University reserves the right to cancel the course, in
which case the enrollment fee will be refunded to the applicant.
Course Directors
Byron S. Tepper, Ph.D., CSP, CBSP is the former Director of the Office
of Safety and Environmental Health of The Johns Hopkins University and
The Johns Hopkins Hospital. He has held academic appointments in the
Departments of Pathobiology and Environmental Health Sciences in The
Johns Hopkins University School of Hygiene and Public Health. He is
currently an Assistant Professor of Occupational Medicine, The Johns
Hopkins University School of Medicine. He is a Fellow of the American
Academy of Microbiology. Dr. Tepper is a microbiologist who entered the
field of biosafety after 15 years of research on leprosy and other
mycobacterial diseases. He is a Certified Safety Professional (CSP) and
a Certified Biological Safety Professional (CBSP) with more than 25
years experience in biosafety, occupational safety and environmental
health. He developed and has continuously directed the course "Control
of Biohazards in the Research Laboratory" which has been presented at
Johns Hopkins since 1979. He is a charter member and a Past-President
of the American Biological Safety Association (ABSA) and currently chair
of the ABSA Biological Safety Professional Certification Committee. He
is past president of the ABSA Chesapeake Area Chapter and the Campus
Safety Association (CSA). In 1998, Dr. Tepper was presented the American
Biological Safety Association's Arnold G. Wedum Distinguished
Achievement Award. Dr. Tepper is a recognized consultant in biosafety,
laboratory safety, laboratory design and hospital safety. He is
currently the CEO of BioControl, Inc., environmental health and safety
consultants.
Richard W. Gilpin, Ph.D., RBP, CBSP is the Head of the Biosafety
Division, Department of Health, Safety and Environment at The Johns
Hopkins University and The Johns Hopkins Hospital. He is an Assistant
Professor of Medicine at The Johns Hopkins University School of Medicine
and an Assistant Professor of Environmental Health Science at The Johns
Hopkins University School of Hygiene and Public Health. Dr. Gilpin is a
basic/clinical/industrial research microbiologist with more than 28
years experience in research, product development and environmental
health. He joined Hopkins twelve years ago after managing a department
of research and development for Becton Dickinson and Company. Dr.
Gilpin has directed microbiology courses for second year medical
students, graduate students, and pharmaceutical company marketing
personnel. He is a Registered Biological Safety Professional, a
Certified Biological Safety Professional, and Councilor (elected
Officer) of the American Biological Safety Association. He is a past
president of the Chesapeake Area Biological Safety Association and a 33
year member of the American Society for Microbiology (Certified in
Biological Safety Microbiology by the American Academy of
Microbiology). Dr Gilpin is also a member of the American Society of
Safety Engineers and the American Academy for the Advancement of
Science. He is a recognized expert on quantitative testing of
Legionella species in warm water containing mechanical equipment. His
consulting in environmental microbiology includes environmental
microbiology, indoor air quality, and laboratory and hospital safety.
For Further Information Contact
Dr. Byron S. Tepper Dr. Richard W. Gilpin
Phone: 410.828.6330 Phone: 410.955.5918
Fax: 410.828.6331 Fax: 410.955.5929
Email: btepper@ Email: gilpin@jhmi.edu
Course Registration Form - May 2000
Please mail remittance and completed registration to:
Becky Vaeth, Registrar Telephone: 410.955.9213
Center for Occupational and Environmental Health
The Johns Hopkins University
2024 E Monument Street, Suite 1-300
Baltimore, MD 21205-2223
Name:__________________________________________________
Address:________________________________________________
City:_____________________ State:_____ Zip Code:____________
Telephone Number: ( )________________________________
Fax ( )____________________________________________
Social Security Number ___________________________________
Employer_______________________________________________
Make checks payable to: JHU Biohazards Course. The Johns Hopkins
University Federal ID is 52-0595110
Check or purchase order must accompany registration form to guarantee
enrollment in the course.
If you have special needs or dietary requirements, please let us know so
that arrangements can be made to accommodate you
(Continued)
Hotel Reservation Form
Please complete and mail to:
Omni Inner Harbor Hotel Telephone: 410.752.1100
101 W. Fayette Street Fax: 410.625.9646
Baltimore, MD 21201
I am enrolled in the Biohazards Course presented by the Johns Hopkins
University, May 1-5, 2000. Please make the following reservation for
me.
Rates- $159 Single or Double, per night.
____________Single(s) ___________Doubles for___________ nights
Reservations rate guaranteed until April 14, 2000.
Name ________________________________________________________
Address
______________________________________________________
City ______________________State________________ Zip Code
__________
Area Code/ Telephone Number ( )
______________________________
Arrival Date __________________ Departure Date
_____________________
Please hold my reservation for late arrival __________ Reservations may
be guaranteed for late arrival by one night's deposit. Include check
payable to the hotel or use major credit card.
AMEX________ VISA_________ MASTER CARD ____________
Card #____________________________Exp. Date____________________
Signature____________________________________ Date______________
=========================================================================
Date: Tue, 28 Dec 1999 09:29:36 -0700
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Therese M. Stinnett"
Subject: waste water lines
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
What direction do you provide to plumbers regarding tying into waste lines
from lab sinks? We limit sink disposal for all our labs. This one in
particular is working with HIV strains and they autoclave virtually
everything.
My standard guidance is to use the same precautions, no matter the lab.
Mucous membrane protection--face shield preferably, or at least goggles. Be
aware of sharp edges, and sharp items that may have ended up in the waste
lines. Be aware of the potential for mercury contamination, especially when
traps are being opened (from ancient times, when labs may not have called
for assistance in cleaning up a broken thermometer...) Immediately report
any cuts or abrasions to the supervisor and report to the occ health clinic.
What am I forgetting?
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
4200 E. 9th Ave.
Denver, CO 80262
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
=========================================================================
Date: Tue, 28 Dec 1999 08:42:47 -0800
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Funk, Glenn"
Subject: Re: waste water lines
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Hi, Terri -
You might also suggest puncture or tear-resistant gloves and a disposable
(Tyvek) closed-front gown, especially if they're working at or above waist
height. Depends on the extent to which you want to carry PPA/PPE. Your
other recommendations sound very reasonable to me.
Hope your Holidays have been great. Best wishes for a wildly successful and
fulfilling Y2K.
- Glenn
-----Original Message-----
From: Therese M. Stinnett [mailto:Therese.Stinnett@UCHSC.EDU]
Sent: Tuesday, December 28, 1999 8:30 AM
To: BIOSAFTY@MITVMA.MIT.EDU
Subject: waste water lines
Importance: High
What direction do you provide to plumbers regarding tying into waste lines
from lab sinks? We limit sink disposal for all our labs. This one in
particular is working with HIV strains and they autoclave virtually
everything.
My standard guidance is to use the same precautions, no matter the lab.
Mucous membrane protection--face shield preferably, or at least goggles. Be
aware of sharp edges, and sharp items that may have ended up in the waste
lines. Be aware of the potential for mercury contamination, especially when
traps are being opened (from ancient times, when labs may not have called
for assistance in cleaning up a broken thermometer...) Immediately report
any cuts or abrasions to the supervisor and report to the occ health clinic.
What am I forgetting?
Therese M. Stinnett
Biosafety Officer
Health and Safety Division
UCHSC, Mailstop C275
4200 E. 9th Ave.
Denver, CO 80262
Phone: 303-315-6754
Pager: 303-266-5402
Fax: 303-315-8026
=========================================================================
Date: Tue, 28 Dec 1999 10:48:29 -0600
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: "Michael R. Betlach"
Subject: UV Lights - and BSC training
MIME-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Stuart Thompson wrote:
>What do list members think about UV lights in PCR cabinets? These are
>claimed to inactivate stray nucleic acids so that your amplified nucleic
>cid is really that which was placed in the sample vial at the scene of the
>rime and did not arise from a piece of dandruff shed by the previous user
>f the cabinet.
Our researchers here request UV lights for exactly this purpose, to
inactivate residual nucleic acids. Many of the BSCs are used for initial DNA
or RNA isolation steps from blood or other materials. The scientists use
chemical disinfectants to inactivate biological materials, but generally
regard them as ineffective for removal of nucleic acids (with the exception
of formaldehyde, possibly glutaraldehyde--which isn't used here--and strong
bases for RNA). Much as I might believe that physical cleaning is sufficient
to remove residual contamination, the exquisite sensitivity of PCR is
perceived as requiring extraordinary measures such as UV lights. Yes, the
newer cabinets we purchase have UV lights interlocked both to the sash and
to the fluorescent lights for routine illumination. Our certifier checks
lamp output yearly. My recollection from the certification reports is that
annual replacement is adequate to maintain lamp output at or above the 25
microwatt per square centimeter value (with the newer lamps).
(Of course, some of the same researchers use PCR cabinets that have UV
lights but no HEPA filtration at all. Apparently they're not concerned about
airborne contaminants they sweep in and out of the box during normal use.)
Re: BSC training videos, I recall an older NIH slide/filmstrip program that
was more to the point than the Eagleson video. As far as I know, it is out
of print and hasn't been converted to an electronic format. Although not
focused on BSC use, one video I've found quite useful is the by the NCCLS
"Protect Yourself: Preventing Infectious Disease Transmitted by Blood, body
Fluids, and Tissue." Although focused on the clinical lab, it has a very
professional tone and presents information at a pace and a level appropriate
for our B.S. through Ph.D. staff.
Storage of suppies in the BSC is a common problem here (especially in the
bact. labs), as is the use of aspirator lines snaked over the front grill
and the all-too-familiar L-shaped configuration (tissue culture labs) in
which a lab cart with supplies is parked end on at one side of the BSC to
increase the ease of moving in and out of the BSC to retrieve supplies,
pipettes, etc. We fixed the aspirator line problem by using a petcock
connected to a vacuum pump. Persistence of the other practices indicates our
training needs to be more direct. We'll try good hood/bad hood slides,
probably excerpts from the Eagleson videos (including the Safe Use of
Chemical Fume Hoods video--for the 'smoke studies' showing airflow
disruption from normal operations--walking by the hood, opening doors,
etc.), and possibly the HHMI Mammalian Cell Culture Hazards video.
Michael Betlach, Ph.D.
Biosafety Officer
Promega Corporation
5445 E. Cheryl Parkway
Madison, WI 53711
(608) 274-1181, Ext. 1270
(608) 277-2677 FAX
mbetlach@
-----Original Message-----
=========================================================================
Date: Tue, 28 Dec 1999 13:36:33 -0500
Reply-To: A Biosafety Discussion List
Sender: A Biosafety Discussion List
From: Greg Merkle
Organization: Wright State University
Subject: Re: waste water lines
MIME-version: 1.0
Content-type: text/plain; charset=us-ascii
Content-transfer-encoding: 7bit
Awareness training dealing with the potential for an
exposure to bloodborne pathogens would be benefitial. Would
the plumbers be considered to be a category of employees
that may be at risk of exposure to bloodborne pathogens? I
would also have plumbers wearing protective gloves if they
are not already doing so. I would suggest having
information on what people are currently using in the lab
that may have gone down the drain (Right-to-Know).
Greg Merkle
Senior Industrial Hygienist
"Therese M. Stinnett" wrote:
>
> What direction do you provide to plumbers regarding tying into waste lines
> from lab sinks? We limit sink disposal for all our labs. This one in
> particular is working with HIV strains and they autoclave virtually
> everything.
>
> My standard guidance is to use the same precautions, no matter the lab.
> Mucous membrane protection--face shield preferably, or at least goggles. Be
> aware of sharp edges, and sharp items that may have ended up in the waste
> lines. Be aware of the potential for mercury contamination, especially when
> traps are being opened (from ancient times, when labs may not have called
> for assistance in cleaning up a broken thermometer...) Immediately report
> any cuts or abrasions to the supervisor and report to the occ health clinic.
>
> What am I forgetting?
>
> Therese M. Stinnett
> Biosafety Officer
> Health and Safety Division
> UCHSC, Mailstop C275
>
> 4200 E. 9th Ave.
>
> Denver, CO 80262
>
> Phone: 303-315-6754
> Pager: 303-266-5402
> Fax: 303-315-8026
................
................
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