Diagnostic approach to Anemia - University of Utah
嚜澳iagnostic approach to Anemia
Archana M Agarwal, M.D.
Department of Pathology
University of Utah School of Medicine
Outline
? Basic hematological parameters
? Discuss types of anemia and their clinical
manifestations
? Talk about the relevant diagnostic tests
Basic hematologic lab tests
? Complete blood count (CBC)
每
每
每
每
Amount of hemoglobin
Number, size, and shape of red blood cells (RBCs)
Number of white blood cells (WBCs) and platelets
+/- automated WBC differential
? Manual differential/manual peripheral smear review
? Abnormalities that fall outside of established parameters result
in manual review
Complete blood count (CBC)
? Hemoglobin
(g/dL)
Amount of oxygen carrying protein
? Hematocrit
(%)
% of blood volume occupied by RBCs
? RBC count
(M/uL)
# of RBCs
? MCV
(fL)
mean cell volume
? MCH
(pg)
mean cell hemoglobin
? MCHC
(g/dL)
concentration
? RDW
? WBC
(%)
mean cell hemoglobin
red cell distribution width
(K/uL)
? Platelet count (K/uL)
# of WBCs
# of platelets
Measuring RBC parameters
?
Hemoglobin (Hb)
每 Measured directly as absorbance of cyanomethemoglobin
?
RBC count (RBC)
每 Measured directly by impedance
?
Hematocrit (Hct)
每 Measured by centrifugation; ratio of volume of RBCs to volume of whole blood
每 Can also calculate (MCV x RBC)
?
MCV
每 Measured by mean height of voltage pulses in an impedance counter
每 Can also calculate (Hct / RBC)
?
MCH = Hb / RBC
?
MCHC = Hb / Hct
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