P3.2.1.DesignDigestive
This is the most problematic step of the entire procedure. Be sure to get full resuspension: 3h-o/n shaking on vortexer at 5 at 37C may work. Remove 25ul of solution to run silver stain gel to measure yield. 2. Add 5ul 1M ammonium bicarbonate. (to 50mM) (Assume previous NH4HCO3 has lost its buffering capacity.) Add 1ul . 100mM. calcium chloride ... ................
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