JEPPIAAR ENGINEERING COLLEGE

JEPPIAAR ENGINEERING COLLEGE

B.TECH ? BIOTECHNOLOGY (R- 2013) BT6504 ? MOLECULAR BIOLOGY III YEAR & V SEM BATCH: 2016-2020

QUESTION BANK

PREPARED BY Mr. G. GOMATHI SANKAR

G. Gomathi Sankar

S.N

TOPIC

REFER PAGE

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ENCE

NO

UNIT I ? CHEMISTRY OF NUCLEIC ACIDS

1 Introduction to nucleic acids

TB1

79

2 Nucleic acids as genetic material

TB1

79

3 Structure and physicochemical properties of elements in DNA and RNA

TB1

80-82

4 Biological significance of differences in DNA and RNA

TB1

84-92

5 Primary structure of DNA: Chemical and structural qualities of 3',5'-

TB1

94-96

Phosphodiester bond

6 Secondary Structure of DNA: Watson & Crick model

TB1

97-100

7 Chargaff's rule

TB1

109

8 X?ray diffraction analysis of DNA, Forces stabilizes DNA structure

TB1

110-112

9 Conformational variants of double helical DNA, Hogsteen base pairing

TB1

114

10 Triple helix, Quadruple helix, Reversible denaturation and hyperchromic effect TB1

112-114

11 Tertiary structure of DNA: DNA supercoiling.

TB1

148

UNIT II ? DNA REPLICATION & REPAIR

1 Overview of Central dogma

TB1

208

2

Organization of prokaryotic and eukaryotic chromosomes DNA replication:

TB1

209

Meselson & Stahl experiment, bi?directional DNA replication

3 Okazaki fragments

TB1

210

4 Proteomics of DNA replication, Fidelity of DNA replication

TB1

224-231

5 Inhibitors of DNA replication

TB1

232-235

6 Overview of differences in prokaryotic and eukaryotic DNA replication

TB1

271-273

7 Telomere replication in eukaryotes

TB1

239-246

8 D-loop and rolling circle mode of replication

TB1

255-258

9 Mutagens, DNA mutations and their mechanism, various types of repair

TB1

293-305

mechanisms

UNIT III ? TRANSCRIPTION

1 Structure and function of mRNA, rRNA and tRNA

TB1

117

2 Characteristics of promoter and enhancer sequences

TB1

316

3 RNA synthesis: Initiation, elongation and termination of RNA synthesis,

TB1

317-329

Proteins of RNA synthesis

4 Fidelity of RNA synthesis

TB1

342

5 Inhibitors of transcription, Differences in prokaryotic and eukaryotic

TB1

343-349

transcription

6 RNA processing: 5'-Capping, Splicing-Alternative splicing, Poly `A' tail

TB1

352-363

addition and base modification

UNIT IV ? TRANSLATION

1

Introduction to Genetic code: Elucidation of genetic code, Codon degeneracy TB1

367-378

2 Wobble hypothesis and its importance

TB1

379

3 Prokaryotic and eukaryotic ribosomes

TB1

439-447

4

Steps in translation: Initiation, Elongation and termination of protein synthesis TB1

425-435

5 Inhibitors of protein synthesis. Post- translational modifications and its

TB1

441-445

importance

UNIT V ? REGULATION OF GENE EXPRESSION

1 Organization of genes in prokaryotic and eukaryotic chromosomes,

TB1

453-454,

Hierarchical levels of gene regulation

502-510

2 Prokaryotic gene regulation ?lac and trp operon

TB1

456-462,

479 - 480

3 Regulation of gene expression with reference to phage life cycle

TB1

598 - 613

G. Gomathi Sankar

TB1 - Friefelder, David. " Molecular Biology." Narosa Publications, 1999

BT6504

MOLECULAR BIOLOGY LT P C

3 0 0 3

OBJECTIVES: Familiarize students with the cell and molecular biology of both Prokaryotes and Eukaryotes. This will be needed for any project work in modern biotechnology. By doing this course students will acquire basic fundamental knowledge and explore skills in molecular biology and become aware of the complexity and harmony of the cells. This course will emphasize the molecular mechanism of DNA replication, repair, transcription, protein synthesis and gene regulation in various organisms.

UNIT I CHEMISTRY OF NUCLEIC ACIDS

9

Introduction to nucleic acids: Nucleic acids as genetic material, Structure and physicochemical properties of elements in DNA and RNA, Biological significance of differences in DNA and RNA. Primary structure of DNA: Chemical and structural qualities of 3',5'-Phosphodiester bond. Secondary Structure of DNA: Watson & Crick model, Chargaff's rule, X?ray diffraction analysis of DNA, Forces stabilizes DNA structure, Conformational variants of double helical DNA, Hogsteen base pairing, Triple helix, Quadruple helix, Reversible denaturation and hyperchromic effect. Tertiary structure of DNA: DNA supercoiling.

UNIT II DNA REPLICATION & REPAIR

9

Overview of Central dogma. Organization of prokaryotic and eukaryotic chromosomes. DNA replication: Meselson & Stahl experiment, bi?directional DNA replication, Okazaki fragments, Proteomics of DNA replication, Fidelity of DNA replication, Inhibitors of DNA replication, Overview of differences in prokaryotic and eukaryotic DNA replication, Telomere replication in eukaryotes. D-loop and rolling circle mode of replication. Mutagens, DNA mutations and their mechanism, various types of repair mechanisms.

UNIT III TRANSCRIPTION

9

Structure and function of mRNA, rRNA and tRNA. Characteristics of promoter and enhancer sequences. RNA synthesis: Initiation, elongation and termination of RNA synthesis, Proteins of RNA synthesis, Fidelity of RNA synthesis, Inhibitors of transcription, Differences in prokaryotic and eukaryotic transcription. Basic concepts in RNA world: Ribozymes, RNA processing: 5'Capping, Splicing-Alternative splicing, Poly `A' tail addition and base modification.

UNIT IV TRANSLATION

9

Introduction to Genetic code: Elucidation of genetic code, Codon degeneracy, Wobble

hypothesis and its importance, Prokaryotic and eukaryotic ribosomes. Steps in translation:

Initiation, Elongation and termination of protein synthesis. Inhibitors of protein synthesis. Post-

translational modifications and its importance.

G. Gomathi Sankar

UNIT V REGULATION OF GENE EXPRESSION

9

Organization of genes in prokaryotic and eukaryotic chromosomes, Hierarchical levels of gene regulation, Prokaryotic gene regulation ?lac and trp operon, Regulation of gene expression with reference to phage life cycle.

TOTAL : 45 PERIODS

OUTCOMES: By the end of this course, students should be able to:

Describe the basic structure and biochemistry of nucleic acids and proteins and discriminate between them;

Identify the principles of DNA replication, transcription and translation and explain how they relate to each other.

Discuss clearly about gene organization and mechanisms of control the gene expression in various organisms.

Articulate applications of molecular biology in the modern world.

TEXT BOOKS: 1. Friefelder, David. " Molecular Biology." Narosa Publications, 1999. 2. Weaver, Robert F. " Molecular Biology" 2nd Edition, Tata McGraw-Hill,2003. 3. Karp, Gerald " Cell and Molecular Biology : Concepts and Experiments" 4th Edition, John Wiley, 2005. 4. Friefelder, David and George M. Malacinski "Essentials of Molecular Biology" 2nd Edition, Panima Publishing, 1993. 5. Lewin's GENES XI, Published by Jones & Bartlett Learning; 11 edition (January 15, 2013). REFERENCES: 1. Tropp, Burton E. " Molecular Biology : Genes to Proteins". 3rd Edition. Jones and Bartlett, 2008. 2. Glick , B.R. and J.J. Pasternak. "Molecular Biotechnology : Principles and Applications of Recombinant DNA" 4th Edition. ASM, 2010.

BT 6504 ? MOLECULAR BIOLOGY NOV/DEC 2017

1. Differentiate primary and secondary structure of DNA

Primary structure: Sequence of nucleotide chains. It is in these channels where the genetic information, and because the skeleton is the same for all the difference in the information lies in the different sequence of nitrogenous bases. This sequence has a code, which determines an information or otherwise, as the order of the bases.

Secondary structure: It is a double helix structure. Can explain the storage of genetic information and the mechanism of DNA

G. Gomathi Sankar

replication. It was postulated by Watson and Crick, based on X-ray diffraction that Franklin and Wilkins had been made, and the equivalence of bases Chargaff, whereby the sum of adenines more guanines is equal to the sum of thymines more cytokines. It is a double strand, right-handed or left-handed, depending on the DNA. Both chains are complementary, as adenine and guanine in a chain are joined, respectively, thymine and cytosine on the other. Both chains are antiparallel, then the 3 'end of one faces the 5' end of the counterpart.

2. What is hogsteen base pairing?

A Hoogsteen base pair is a variation of base-pairing in nucleic acids such as the A. ? T pair. In this manner, two nucleobases, one on each strand, can be held together by hydrogen bonds in the major groove.

3. Complete the following, label A, B and C and name the process central (Dogma)

A ? Replication

B- Transcription

C- Translation

4. What is bidirectional replication?

DNA is double stranded molecule. Only one strand codes for proteins at any given point in the molecule. However, both strands are used during DNA replication. Each of the four bases in DNA (adenine, thymine, guanine, and cytosine) binds to a unique complementary base on the other strand. Therefore the base sequence on one strand determines the complementary sequence on the other strand. During DNA replication the two strand separate from one another and each strand has a new complementary strand built onto it. This form of replication is called bi directional; also called as semi conservative; each new DNA molecule is composed of one conserved strand from the original molecule and one new strand.

5. Which is most unstable type of RNA molecule ? why ? mRNA is the most unstable RNA .

G. Gomathi Sankar

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