Fundamentals: Nucleic acids, DNA replication ...

Fundamentals: Nucleic acids, DNA replication, transcription, translation and

application to molecular detection

Prokaryotic cell

Binary Fission

? Bacteria reproduce asexually via binary fission

? Each daughter cell is an identical copy (or clone) of its parent cell

Microbial evolution 101

Generation 1 Generation 2 Generation 3 Generation N

Ancestor Genotype

Clones

Clones Clones and Divergent Genotypes

Microbial genetics 101

? What is DNA ? ? What types of DNA molecules

are present in a bacterial cell? ? What's the size of the genetic

material for a typical bacterial pathogen ? ? How many genes does a bacterial pathogen have ? ? What's the average size of a bacterial gene ?

What Is DNA?

Double Helix Structure and Antiparallel Orientation

Nucleotide = 5 carbon sugar + nitrogenous base + phosphate group

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