DNA Technology Worksheet



DNA Technology NAME:________________________

1. Name two situations when DNA fingerprints are useful.

_________________________________ and _______________________________

2. How does the DNA migrate from one end of the gel to the other?

3. What cuts up the DNA into tiny fragments? _____________________________________

Use the gels below to answer the following questions.

Multiple Choice

____ 1. To produce genetically engineered bacteria that make a human protein, which of the following steps does a scientist have to take first?

|a. |Insert the human gene for the protein into a plasmid. |

|b. |Extract the protein from the bacterial culture. |

|c. |Use a restriction enzyme to cut out the gene from human DNA. |

|d. |Transform bacteria with the recombinant plasmid. |

____ 2. During genetic modification,

|a. |a prokaryote is changed into a eukaryote. |

|b. |a cell takes in DNA from another source. |

|c. |a cell’s own DNA is inserted into a plasmid. |

|d. |a cell is mutated. |

____ 3. Analyzing DNA by gel electrophoresis allows researchers to

|a. |compare DNA samples from different sources. |

|b. |determine whether a particular allele of a gene is dominant or recessive. |

|c. |compare the phenotypes of different organisms. |

|d. |cut DNA with restriction enzymes. |

____ 4. One function of gel electrophoresis is to

|a. |separate DNA fragments. |

|b. |cut DNA. |

|c. |recombine DNA. |

|d. |extract DNA. |

____ 5. Knowing the sequence of an organism’s DNA allows researchers to

|a. |reproduce the organism. |

|b. |mutate the DNA. |

|c. |study specific genes. |

|d. |cut the DNA. |

____ 6. Which of the following includes all the others?

|a. |plasmid |

|b. |genetically modified bacterium |

|c. |foreign gene |

|d. |recombinant DNA |

____ 7. On an electrophoresis gel, band B is closer to the positive end of the gel than is band A. Which of the following statements is true?

|a. |Band B is more negatively charged than band A. |

|b. |Band B moved faster than band A. |

|c. |Band A is smaller than band B. |

|d. |Band B consists of larger DNA fragments than does band A. |

____ 8. If two DNA samples showed an identical pattern and thickness of bands produced by gel electrophoresis, the samples contained

|a. |the same amount of DNA. |

|b. |fragments of the same size. |

|c. |the same DNA molecules. |

|d. |all of the above |

____ 9. What is an advantage of using genetically engineered bacteria to produce human proteins?

|a. |The human proteins produced by genetically engineered bacteria work better than those produced by humans. |

|b. |Genetically engineered bacteria can mass-produce pure human proteins. |

|c. |The human proteins produced by genetically engineered bacteria last longer than those produced by humans. |

|d. |Genetically engineered bacteria can produce human proteins used to make plastics. |

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dad 1

mom

baby

dad 2

4. Mrs. Smith has a baby named Jessica. She believes one of two men can be the father of her child. A paternity test is done and the results are shown above. Which of the 2 men are baby Jessica’s father?

S2

felon

S3

S1

6. Suzy was assaulted in an alley. The police collected a sample of cells that was left at the crime scene and now have 3 suspects in custody. Which of the suspects assaulted Suzy?

S2

felon

S3

S1

5. Lt. Russ is investigating a murder scene. The felon was scratched by his victim & some of his skin cells were found under the victim’s fingernails. A DNA test was performed. Which of the suspects is the murderer?

dad

child

1

mom

child

2

child

3

child

4

child

5

child

6

7. Mr. & Mrs. Jones just gave birth to fraternal twins- Bob and Jane. Unfortunately, the nurse has confused the Jones twins with 4 other babies. The doctors took samples of DNA from each of the babies and Mr. & Mrs. Jones. Which of the 6 children are Mr. & Mrs. Jones twins?

mom

son 1

daughter

son 2

8. The millionaire, Mr. Big, has just died. He has left behind a wife, daughter and a large inheritance. The news of his death has brought forth 2 men who claim to be the long lost son of Mr. & Mrs. Big. Before Mr. & Mrs. Big were married they had a child taken from them. They had tried to find him but had no luck in locating him. A DNA sample was taken from Mrs. Big, the Big daughter and the two men who claim to be the long lost son. Which, if any, of the men are telling the truth?

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