DNA Structure and Chromosome Organization Questions
DNA Structure and Chromosome Organization Questions
1. If a double-stranded DNA molecule has 15% thymine, what are the percentages of all the other bases?
2. A B-DNA molecule has 1 million nucleotide pairs
How many complete turns are there in this molecule?
3. Describe in steps how the double helix of DNA, which is 2 nm in width, gives rise to a chromosome that is 700 nm in width.
Answers to DNA Structure and Chromosome Organization Questions
1. The percentage of thymine (15%) should be equal to the percentage of adenine (15%). The remaining percentage of DNA bases will consist of cytosine and guanine (100-30% = 70%); these should be in equal mounts (70%/2 = 35%). So, the percentage of each of the other bases if the thymine content is 15% are:
adenine = 15%
guanine = 35%
cytosine = 35%
2. B-form DNA contains approximately 10 nucleotides per turn of the helix. A B-DNA molecule of 1 million nucleotide pairs will have about the following number of complete turns:
1,000,000 nucleotides/ 10 nucleotides/turn = 100,000 complete turns.
3. DNA is first packaged into nucleosomes; the nucleosomes are packed to form a solenoid (30 nm fiber). The 30 nm fiber forms a series of loops that pack to form a 300 nm fiber, which in turn coils to form a 700 nm chromatid.
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