Pennsylvania State University

For this purpose, fish maintained at 28.5° C under a photoperiod of 14:10 hours light:dark, with continuously filtered water at pH 7-7.8, conductivity at 500-800 µS and O. 2. dissolved at 60 - 90 %, were crossed and fertilized eggs collected and incubated in E3 with ampicillin (100 µg/ml) and methylene blue (0.0001%) until 3d ................
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