MANAGEMENT PLAN - Angelfire
Thus, at least 10 replicates will be amplified for only 15 cycles to minimize skewing of the distribution of sequence types and accumulation of mutations and chimeric molecules. Reactions will be diluted 1:10 into fresh reagents and amplified for 3 cycles to remove heteroduplex molecules (Thompson et al. 2002) followed by pooling and cloning. ................
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