Electro-competent
The Preparation of E. coli (BJ5183) by Electroporation(06-24-2005 wyy) Place the competent cells on LB plate, 37°C overnight (16-20h). Pick a single bacterial colony from a plate that has been incubated for 16-20 hours at 37°C. Transfer the colony into 20 ml of LB medium in a 100-ml flask. ................
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