ANNUAL RESEARCH DAY PROGRAM - University of Ottawa



[pic] [pic] [pic]

DEPARTMENT OF PATHOLOGY AND LABORATORY MEDICINE

DÉPARTEMENT DE PATHOLOGIE ET DE MÉDECINE DE LABORATOIRE

JOURNÉE ANNUELLE

DE LA RECHERCHE

ANNUAL RESEARCH DAY

2017

[pic]

ANNUAL RESEARCH DAY PROGRAM

DEPARTMENT OF PATHOLOGY AND

LABORATORY MEDICINE

UNIVERSITY OF OTTAWA

Monday May 15th, 2017

ROGER GUINDON HALL

ROOM 1007

HEALTH SCIENCES BUILDING

8:45 - 9:00 COFFEE

9:00 – 9:10 WELCOME

9:10 – 9:25 THE USE OF A TISSUE SAVING MULTIPLEX ANTIBODY (ADH5) WITH TTF 1 TO SUBTYPE NON-SMALL CELL LUNG CANCER ON CYTOLOGY SPECIMENS

Jordan Sim, Shahid Islam. Department of Pathology and Laboratory Medicine, University of Ottawa and The Ottawa Hospital, Ottawa ON, Canada

9:25 –9:40 IFITM1 OUTPERFORMS CD10 IN DIFFERENTIATING LOW GRADE ENDOMETRIAL STROMAL SARCOMAS FROM SMOOTH MUSCLE NEOPLASMS OF THE UTERUS

Aurelia Busca1, Previn Gulavita2, Carlos Parra-Herran3* and Shahidul Islam1*, 1 Department of Pathology and Laboratory Medicine, University of Ottawa and The Ottawa Hospital, Ottawa ON, Canada, 2 Department of Medical Biology, University of Ottawa and Montfort Hospital, Ottawa ON, Canada, 3 Department of Laboratory Medicine and Pathobiology, University of Toronto and Sunnybrook Health Sciences Centre, Toronto ON, Canada

9:40-9:55 NSCLC LYMPH NODE METASTASIS: DO CYTOMORPHOLOGICAL FEATURES CORRELATE WITH STANDARDIZED UPTAKE VALUE OF PET SCAN?

Kianoosh Keyhanian, Harman Sekhon

Department of Pathology and Laboratory Medicine, University of Ottawa/The Ottawa Hospital, Ottawa, Ontario

9:55-10:10 LIPOMATOUS TUMOURS WITH ATYPIA ARE NOT ALWAYS LIPOSARCOMA: IMMUNOHISTOCHEMICAL EVALUATION OF A SERIES OF LIPOMATOUS TUMOURS WITH ATYPIA, INCLUDING A RETROPERITONEAL PLEOMORPHIC LIPOMA

Ashley N. Flaman a,b, Denis H. Gravel a,b, Chi K. Lai a,b, Susan J. Robertson a,b, Bibianna M. Purgina a,b

aDepartment of Pathology and Laboratory Medicine, The Ottawa Hospital, Ottawa, ON; bUniversity of Ottawa, Ottawa, ON

10:10-10:25 VALUE OF EOSINOPHILIC ESOPHAGITIS HISTOLOGY SCORING SYSTEM

Chernetsova, Elizaveta1, A Agarwal2, D El Demellawy1,2

University of Ottawa, Children’s Hospital of Eastern Ontario, Canada

10:25 – 10:45 COFFEE BREAK

10:45-11:00 THE HISTOPATHOLOGY AND THE PATHOGENESIS OF FLOPPY EYELID SYNDROME

Chen, Henry1, 2; Brownstein, Seymour1, 2; Jordan, David R.1; Belliveau, Michel J.1; Gilberg, Steven1; Blanco, Paula2; Farmer, James2; Iacob, Codrin3

1. Ophthalmology, University of Ottawa, Ottawa, ON, Canada. 2. Pathology & Laboratory Medicine, University of Ottawa, Ottawa, ON, Canada. 3. Pathology, New York Eye and Ear Infirmary, New York, NY, United States.

11:00-11:15 AN ANALYSIS OF MEDICAL ASSISTANCE IN DYING CASES IN ONTARIO:  UNDERSTANDING THE PATIENT DEMOGRAPHICS OF CASE UPTAKE IN ONTARIO SINCE THE ROYAL ASSENT AND AMENDMENTS OF BILL C-14

Rosso, Alexandra E BHSc candidate, School of Health Sciences, University of Ottawa

Dr Dirk Huyer MD, Chief Coroner for the Province of Ontario, Office of the Chief Coroner

Dr Alfredo Walker MB.BS, FRCPath, DMJ (Path), MFFLM, MCSFS.Forensic Pathologist/Coroner – Ontario Forensic Pathology Service. Eastern Ontario Regional Forensic Pathology Unit – Ottawa. Department of Pathology and Laboratory Medicine, University of Ottawa

11:15-11:30 OOCYTE DONATION PREGNANCIES AND THE RISK OF PREECLAMPSIA OR GESTATIONAL HYPERTENSION: A SYSTEMATIC REVIEW AND META-ANALYSIS

Masoudian, Pourya, BHSc1 , Ahmed NASR, MD, MSc2, Joseph De Nannasy, MD1,, Karen FUNG-KEE-FUNG, MD, MHPE3, Shannon A. BAINBRIDGE, PhD4, Dina EL DEMELLAWY, MD, PhD1

1Department of Pediatric Pathology, Children's Hospital of Eastern Ontario, Faculty of Medicine, University of Ottawa, Ottawa, ON

2Department of Pediatric Surgery, Children's Hospital of Eastern Ontario, Faculty of Medicine, University of Ottawa, Ottawa, ON

3Department of Obstetrics and Gynecology, The Ottawa Hospital, Faculty of Medicine, University of Ottawa, Ottawa ON

4Faculty of Health Sciences, University of Ottawa, Ottawa, ON.

11:30-11:45 TREATMENT MODALITIES OF TWIN ANEMIA POLYCYTHEMIA (TAPS): A SYSTEMATIC REVIEW

Hill, Kevin1, Pourya Masoudian1, Karen Fung-Kee-Fung1,2, Dina El Demellawy1,3

1University of Ottawa, Faculty of Medicine, 2Department of Obstetrics and Gynecology, The Ottawa Hospital, 3Department of Pediatric Pathology, Children's Hospital of Eastern Ontario, Ottawa, Ontario

11:45 – 13:45 LUNCH AND POSTER VIEWING

Atrium (University)

13:45 – 14:45 KEYNOTE LECTURE - Dr. Fattaneh Tavassoli

Professor of Pathology - Yale University, USA

Lecture Title: “Correlation between GEP-based Molecular and Morphologic Classification of Breast Carcinomas”

Objectives: To cover the morphology of multiple variants of triple negative carcinoma, evolution of molecular classification & some of the potential problems if one relies completely on molecular classification.

14:45 – 15:00 POSTER ORAL PRESENTATIONS (OPTIONAL)

14:45-14:50 PEDIATRIC BLASTIC PLASMACYTOID DENDRITIC CELL NEOPLASM - A SYSTEMATIC LITERATURE REVIEW

Jeong-Min Kim, Marie, Ahmed Nasr, Bilaal Kabir, Joseph de Nanassy, Ken Tang, Danielle Menzies-Toman, Donna Johnston, Dina El Demellawy

University of Ottawa, Children’s Hospital of Eastern Ontario, Canada

14:50-14:55 DIAGNOSTIC VALUE OF MID ESOPHAGEAL BIOPSIES IN PEDIATRIC PATIENTS WITH EOSINOPHILIC ESOPHAGITIS

Chernetsova, E, J Joo, A Agarwal, J Barkey, D El Demellawy

University of Ottawa, Children’s Hospital; of Eastern Ontario, Canada

14:55-15:00 A RARE CASE OF PEDIATRIC LIPOMA WITH T(9;12)(P22;Q14) AND EVIDENCE OF HMGA2 NFIB GENE FUSION

Lacaria, Melanie1, Dina El Demellawy2,3, Jean McGowan-Jordan1,4

1Genetics Department, Children’s Hospital of Eastern Ontario;

2Pediatric Pathology Department, Children’s Hospital of Eastern Ontario;

3Department of Surgery, University of Ottawa;

4Department of Pathology and Laboratory Medicine, University of Ottawa

15:00 – 15:15 COFFEE BREAK

15:15 – 15:30 ANNOUNCEMENT OF PRIZE WINNERS AND CONCLUSION

• Nadia Mikhael Award for Best Paper presented by a Junior Resident

• 2nd Best paper by a Junior Resident

• Virbala Acharya Award for Best Presentation by a Senior Resident or Fellow

• 2nd Best paper by a Senior Resident or Fellow

• Best Poster Presentation by a Graduate Student

• 2nd Best Poster Presentation by a Graduate Student

• Best Poster Presentation by a Resident

• 2nd Best Poster Presentation by a Resident

• Dr. M. Orizaga Award for Best Teacher

POSTERS

1. PEDIATRIC BLASTIC PLASMACYTOID DENDRITIC CELL NEOPLASM A SYSTEMATIC LITERATURE REVIEW

Jeong-Min Kim, Marie, Ahmed Nasr, Bilaal Kabir, Joseph de Nanassy, Ken Tang, Danielle Menzies-Toman, Donna Johnston, Dina El Demellawy

University of Ottawa, Children’s Hospital of Eastern Ontario, Canada

2. THE EFFICIENCY OF ACTIVATING THE MasR/Ang1-7 PATHWAY TO REDUCE MUSCLE ATROPHY AND FUNCTION LOSS FOLLOWING DENERVATION

Albadrani, H*1 and J.M Renaud*1

Department of the Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada

3. RNA-SEQ ANALYSIS OF REXINOID RESPONSIVE GENE EXPRESSION DURING EARLY MYOBLAST DIFFERENTIATION

Khilji, Saadia, Qiao Li

Department of the Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada

4. Retrospective, case-controlled stereological assessment of vascularization in placentas of HIV-exposed, uninfected (HEU) children.

Wahba, Mary, Jenifer Bowes (BSc, MSc), Chris G. Ball (M.D.), Jason Brophy (M.D.), David Grynspan (M.D.)

University of Ottawa, Children’s Hospital of Eastern Ontario, Canada

5. DIAGNOSTIC VALUE OF MID ESOPHAGEAL BIOPSIES IN PEDIATRIC PATIENTS WITH EOSINOPHILIC ESOPHAGITIS

Chernetsova, E, J Joo, A Agarwal, J Barkey, D El Demellawy

University of Ottawa, Children’s Hospital; of Eastern Ontario, Canada

6. DOUBLE HIT DIFFUSE LARGE B-CELL LYMPHOMA TESTING AT THE OTTAWA HOSPITAL

Capitano, Mario, M.D., and Philip Berardi, M.D., Ph.D., FRCPC

The Ottawa Hospital, General Campus, 501 Smyth Rd, Ottawa ON K1H 8L6

7. ADENOCARCINOMA EX-GOBLET CELL CARCINOID TUMOURS, PATHOLOGIC CLASSIFICATION AND CLINICAL BEHAVIOR: AN ANALYSIS OF CASE SERIES (FROM 2003-2017 FROM THE OTTAWA HOSPITAL PATHOLOGY GROUP

Capitano, Mario, M.D., and Zohreh Eslami, M.D., Ph.D., FRCPC

The Ottawa Hospital, General Campus, 501 Smyth Rd, Ottawa ON K1H 8L6

8. STROMAL TISSUE AS AN ADJUNCT TOOL IN THE DIAGNOSIS OF FOLLICULAR THYROID LESIONS BY FINE-NEEDLE ASPIRATION BIOPSY

Hogan, Kevin, Kien T. Mai.

The Ottawa Hospital and University of Ottawa, Pathology and Laboratory Medicine, Ottawa, ON, Canada

9. CHARACTERISTICS OF LYMPHOPROLIFERATIVE DISORDERS (LPD) WITH MORE THAN ONE ABERRANT CELL POPULATION AS DETECTED BY 10 COLOR FLOW CYTOMETRY

Mahdi, Talal (1, 2), Amr Rajab (2,3) ,Ruth Padmore (1), *Anna Porwit (2,4)

(1) Department of Pathology and Laboratory Medicine, The Ottawa Hospital and Eastern Ontario Regional Laboratory Association and University of Ottawa, ON, Canada.

(2) Flow Cytometry Laboratory, Department of Laboratory Hematology, Laboratory Medicine Program, University Health Network, Toronto, ON, Canada.

(3) Present address: Flow cytometry department, Lifelabs Medical laboratory services-Toronto.

(4) Present address: Department of Clinical Sciences, Division of Oncology and Pathology, Faculty of Medicine, Lund University, Lund, Sweden

10. EMERGENCY TRANSFUSION OF ONE UNCROSSMATCHED GROUP O Rh POSITIVE RED CELL UNIT TO A PATIENT WITH ANTI-D ANTIBODY IDENTIFY ONCE TYPE AND SCREEN WERE COMPLETED. A CASE REPORT

Mahdi, Talal, MD, Ruth Padmore, MD

The Ottawa Hospital and Eastern Ontario Regional Laboratory Association and University of Ottawa

11. A RARE CASE OF PEDIATRIC LIPOMA WITH T(9;12)(P22;Q14) AND EVIDENCE OF HMGA2 NFIB GENE FUSION

Lacaria, Melanie1, Dina El Demellawy2,3, Jean McGowan-Jordan1,4

1Genetics Department, Children’s Hospital of Eastern Ontario;

2Pediatric Pathology Department, Children’s Hospital of Eastern Ontario;

3Department of Surgery, University of Ottawa;

4Department of Pathology and Laboratory Medicine, University of Ottawa

12. DEPARTMENT OF PATHOLOGY AND LABORATORY MEDICINE

HISTOLOGY CORE FACILITY

J.P.Veinot1, C.A. Jodouin2, Z. Ticas3, L. Dong4, E. Labelle5, S. Faulkes6, M. Khalili7, M. Alazzabi8, O. Agah9

The University of Ottawa, Faculty of Medicine, Department of Pathology and Laboratory Medicine (PALM), Histology Core Facility

WELCOME

THE USE OF A TISSUE SAVING MULTIPLEX ANTIBODY (ADH5) WITH TTF 1 TO SUBTYPE NON-SMALL CELL LUNG CANCER ON CYTOLOGY SPECIMENS

Jordan Sim, Shahid Islam. The Ottawa Hospital, Ottawa, ON, Canada

Background: 70% of lung cancers present at an advanced stage, and subtyping of non-small cell lung cancers must therefore be done on cytologic specimens and small biopsies when possible. Many antibodies have been investigated for the subtyping of non-small cell lung carcinomas. Although the combination of p40 and TTF-1 is commonly used, this is not always sufficient and more markers must be used. Entering the era of personalized medicine, conservation of cell blocks for molecular studies is vital, and minimizing the number of immunohistochemical stains is important. The multiplex antibody ADH5 is frequently used in breast pathology, but to date has not been investigated for its use in lung cancer. ADH5 includes a panel of antibodies (CK5/14, CK7/18 and p63) all of which have been proven to aid in the subtyping of non-small cell lung carcinomas. The aim of this study is to assess the efficacy of the ADH5 multiplex antibody in combination with TTF-1 in subtyping non-small cell carcinomas.

Design: A database search from January 2014 onwards was conducted for cases of cytologically diagnosed non-small cell lung cancer with subsequent surgical resection specimens. A total of 175 consecutive cases were retrieved, 83 of which had adequate cell blocks fixed in formalin. Immunohistochemistry for TTF-1 and ADH5 was performed on all 83 cases.

Results: Of the 83 cases used in this study, final diagnoses on surgical resection included squamous cell carcinoma (25.3%), adenocarcinoma (71.1%) and adenosquamous carcinoma (3.6%). On cytology, 17 (20.5%) cases could not be subtyped. At the time of original cytologic diagnosis, immunohistochemistry was used to subclassify NCSLC in 32 (38.6%) cases. The number of stains performed ranged from 2 to 5 (mean 3.38). With the use of immunohistochemistry, 9 (11%) remained not subtyped. Using the combination of TTF-1 and ADH5 multiplex antibody, 80/83 (96%) cases were classified accurately, including 17 cases that were previously not subtyped and 1 case that was misdiagnosed. 3 (3.6%) cases of adenosquamous carcinoma could not be accurately subtyped using this panel.

Conclusions: A multiplex antibody for CK5/14, CK7/18 and p63 in combination with TTF-1 is an effective combination for subtyping NSCLC on cytology specimens. This combination requires only 2 sections, therefore saving tissue for further ancillary studies.

IFITM1 OUTPERFORMS CD10 IN DIFFERENTIATING LOW GRADE ENDOMETRIAL STROMAL SARCOMAS FROM SMOOTH MUSCLE NEOPLASMS OF THE UTERUS

Aurelia Busca1, Previn Gulavita2, Carlos Parra-Herran3* and Shahidul Islam1* 1 Department of Pathology and Laboratory Medicine, University of Ottawa and The Ottawa Hospital, Ottawa ON, Canada

2 Department of Medical Biology, University of Ottawa and Montfort Hospital, Ottawa ON, Canada

3 Department of Laboratory Medicine and Pathobiology, University of Toronto and Sunnybrook Health Sciences Centre, Toronto ON, Canada

* CP-H and SI contributed equally as senior authors

Introduction: Distinguishing between uterine neoplasms of smooth muscle and endometrial stromal origin is a frequent diagnostic challenge. We investigated the staining pattern of interferon-induced transmembrane protein-1 (IFITM1), a novel endometrial stromal marker, in endometrial and smooth muscle uterine neoplasms and compared it to CD10 in its ability to differentiate between these two groups.

Methods: Immunohistochemistry for IFITM1 and CD10 was performed in 20 cases of smooth muscle neoplasms (10 cases leiomyoma, 10 cases leiomyosarcoma), 14 cases of endometrial stromal sarcoma (12 cases low grade and 2 cases high grade) and 12 cases of carcinosarcoma. Staining was scored in terms of intensity and distribution (0=absent, 1=weak/less than 50%, 2=moderate/50-75%, 3=strong/more than 75%). A total score was obtained by adding intensity and distribution scores and classified as positive (score 3-6) or negative (score 0-2).

Results: IFITM1 was positive in 10/12 (83%) low grade endometrial stromal sarcomas, 6/20 (30%) smooth muscle tumors (leiomyomas and leiomyosarcomas) and 11/12 carcinosarcomas (91.6%). The two cases of high grade endometrial stromal sarcoma were IFITM1 negative. While both IFITM1 (83%) and CD10 (91%) had high sensitivity in differentiating low grade endometrial stromal sarcomas from smooth muscle neoplasms, IFITM1 (70%) had higher specificity compared to CD10 (45%).

Conclusions: In this study IFITM1 appears to be a more specific marker of endometrial stromal differentiation compared to CD10 in differentiating low grade endometrial stromal sarcomas from smooth muscle neoplasms. Thus, IFITM1 may be a valuable tool as part of an immunohistochemical evaluation panel in this diagnostic scenario.

NSCLC LYMPH NODE METASTASIS: DO CYTOMORPHOLOGICAL FEATURES CORRELATE WITH STANDARDIZED UPTAKE VALUE OF PET SCAN?

Kianoosh Keyhanian, Harman Sekhon

Department of Pathology and Laboratory Medicine, University of Ottawa/The Ottawa Hospital, Ottawa, Ontario

 

Background/Objective

Positron emission tomography (PET) followed by endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) of the suspicious lymph nodes (LNs) is a standard procedure for pre-operative staging of lung cancer. The objective of this study is to correlate cytomorphological features of metastatic non-small cell lung carcinoma (mNSCLC) with maximal standardized uptake value (mSUV) of PET scan in LNs.

Method(s)

Positive EBUS-TBNA cytology slides were reviewed from patients with mNSCLC. Fifty-seven LNs were selected from 49 patients who had undergone PET study. Student t-test was used for statistical comparisons.

Results

Mean patients’ age was: 68.7, 67% male. LNs locations were as follows: mediastinum: 48 (24 subcarinal, 24 para-tracheal), lung hilum: 8, axilla: 1. Final diagnoses were: Adenocarcinoma: 41 LNs, squamous cell carcinoma: 13 LNs and NSCLC: 3 LNs. Within the Adenocarcinoma subgroup, we observed that histological patterns correlate with mSUV, with acinar and papillary (both mean values: 9.3) patterns associating with significantly lower mSUVs than solid pattern (Mean value: 13.8) (both P values ................
................

In order to avoid copyright disputes, this page is only a partial summary.

Google Online Preview   Download