SUPPLEMENTAL MATERIAL - Lippincott Williams & Wilkins



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Table 1: Effects of Cecal Ligature and Puncture on Myocardial function as Assessed by Positron Emission Tomography.

| |Sham (n=6) |CLP (n=6) |CLP NE (n=6) |CLP E (n=6) |CLP PE (n=6) |

|HR (beats/min) |404 ± 16 |438 ± 43 |422 ± 47 |427 ± 17 |406 ± 35 |

|CO (mL.min-1) |118 ± 14 |85 ± 12* |76 ± 19* |67 ± 26* |76 ± 21* |

|MAP (mmHg) |110 ± 6 |88 ± 9* |85 ± 8* |88 ± 15* |85 ± 8* |

|LVEF (%) |67 ± 7 |52 ± 2* |49 ± 7* |45 ± 9* |48 ± 12* |

|PER (mL.min-1) |58 ± 5 |41 ± 4* |35 ± 7* |32 ± 8* |43 ± 10* |

|PFR (mL.min-1) |63 ± 14 |43 ± 6* |34 ± 5* |34 ± 6* |40 ± 7* |

|LVEDV (µL) |421 ± 24 |378 ± 28 |338 ± 36 |335 ± 92 |420 ± 47 |

*: p < .05 vs. sham, no difference was observed between CLP groups

CLP: Cecal Ligature and Puncture. CO: Cardiac Output. E: Epinephrine. HR: Heart Rate. LVEDV: Left Ventricular End Diastolic Volume. LVEF: Left Ventricular Ejection Fraction.. MAP: Mean Arterial Pressure. NE: Norepinephrine PE: Phenylephrine. PER: Peak Ejection Rate. PFR: Peak Filling Rate.

Table 2: Total Energy Charge, ATP/ADP Ratio and Total Adenine Nucleotide Pool at t+60 Min in Sham and Septic Rats without Treatment or Treated with Epinephrine, Norepinephrine or Phenylephrine

| |EC |ATP |ATP/ADP |TAN |

|SHAM |0.83 ± 0.03 |15,7 ± 3,8 |5,4 ± 0,8 |21,0 ± 5,0 |

|CLP |0.41 ± 0.06* |3,7 ± 1,4* |2,7 ± 0.9* |9,3 ± 4,7 |

|NE |0.56 ± 0.11* |5,0 ± 2,5* |2,2 ± 0,6* |12,3 ± 5,2* |

|E |0.56 ± 0.03* |6,8 ± 2,5* |2,9 ± 0,9* |13,5 ± 5,2* |

|PE |0.52 ± 0.04* |5,8 ± 2,5* |2,7 ± 0,8* |13,1 ± 6,2* |

*: p < .01 vs. sham, values are means ± SD. Units are nmol.mg-1 (wet weight).

ADP: Adenodiphosphate. ATP: Adenotriphosphate. CLP: Cecal Ligature and Puncture. E: Epinephrine. EC: Energy Charge. NE: Norepinephrine P: Phenylephrine. TAN: Total Adenine Nucleotide pool

Table 3: Comparative Effects of Norepinephrine, Epinephrine and Phenylephrine on Septic Myocardial Dysfunction as Assessed by Positron Emission Tomography

|Parameter |Group |Baseline |Post FR |T+60min |

|HR (beats/min) |NE |422 ± 47 |425 ± 42 |439 ± 24 |

| |E |427 ± 17 |454 ± 17 |484 ± 16*† |

| |PE |406 ± 35 |402 ± 36 |378 ± 61 |

|CO (mL.min-1) |NE |76 ± 19 |86 ± 24 |101 ± 11* |

| |E |67 ± 26 |82 ± 19 |95 ± 12* |

| |PE |76 ± 21 |98 ± 12 |70 ± 35 |

|MAP (mmHg) |NE |85 ± 8 |91 ± 8 |108 ± 8* |

| |E |88 ± 15 |103 ± 6 |111 ± 6* |

| |PE |85 ± 8 |92 ± 5 |106 ± 5* |

|LVEF (%) |NE |49 ± 7 |55 ± 8 |65 ± 5* |

| |E |45 ± 9 |49 ± 11 |61 ± 7* |

| |PE |48 ± 12 |54 ± 8 |42 ± 10† |

|PER (mL.min-1) |NE |35 ± 7 |45 ± 7 |51 ± 2* |

| |E |32 ± 8 |34 ± 10 |43 ± 6* |

| |PE |43 ± 10 |56 ± 6 |43 ± 9† |

|PFR (mL.min-1) |NE |34 ± 5 |45 ± 10 |45 ± 6* |

| |E |34 ± 6 |36 ± 8 |39 ± 9* |

| |PE |40 ± 7 |47 ± 8 |44 ± 11 |

|LVEDV (µL) |NE |338 ± 36 |375 ± 72 |355 ± 62 |

| |E |335 ± 92 |335 ± 106 |323 ± 89 |

| |PE |420 ± 32 |470 ± 34 |492 ± 87 |

*: p < .05 « T+60min » vs. « baseline », †: p< .05 intragroup comparison at t+60min

CO: Cardiac Output. E: Epinephrine. HR: Heart Rate. LVEDV: Left Ventricular End Diastolic Volume. LVEF: Left Ventricular Ejection Fraction.. MAP: Mean Arterial Pressure. NE: Norepinephrine PE: Phenylephrine. PER: Peak Ejection Rate. PFR: Peak Filling Rate. Post FR: Post Fluid Resuscitation.

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