Protocol of results of the ISTA Proficiency test on GMO ...
| |11th ISTA GMO Proficiency Test, Canola/Oil Seed Rape (Brassica sp.) |
|Laboratory Name: | |Laboratory No.: |
| | | |
1. Qualitative Test (Detection)
1.1 Qualitative Results per sample (please tick the boxes)
|sample number |positive |
|1 | |
|2 | |
|3 | |
|4 | |
|5 | |
|6 | |
|7 | |
|8 | |
|9 | |
|10 | |
|11 | |
|12 | |
1.2 Optional information: test method used for qualitative test
|Type of test method | PCR |Specify: |
| | ELISA |Specify: |
| | Bioassay |Specify: |
| | derived from the quantitative test result |
Please provide additional information about the test method (e.g. grinding method; DNA extraction method; target for PCR, ELISA or bioassay; primer sequences; test kit; method publication/validation references etc.).
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Date and signature
Return to the ISTA Secretariat, Zürichstr. 50, 8303 Bassersdorf, Switzerland, by Fax (+41 44 838 60 01) or
email: tcom@ista.ch
| |11th ISTA GMO Proficiency Test, Canola/Oil Seed Rape (Brassica sp.) |
|Laboratory Name: | |Laboratory No.: |
| | | |
2. Quantification of GM-seeds in positive samples
2.A. Sub-sampling Quantification (Semi-quantitative Test)
2.A.1 Sub-sampling Quantification Results
|Sample # |# of sub-samples tested |Size of sub-sample |# of positive |Estimate of % GM seeds |
| | |(# of seeds) |sub-samples | |
|1 | | | | |
|2 | | | | |
|3 | | | | |
|4 | | | | |
|5 | | | | |
|6 | | | | |
|7 | | | | |
|8 | | | | |
|9 | | | | |
|10 | | | | |
|11 | | | | |
|12 | | | | |
2.A.2. Optional information: method used for the semi-quantitative test
|What is the estimated false positive rate and false negative rate for the |false positive rate (%) |false negative rate (%) |
|method used in this test? | | |
| | | |
| | | |
|Type of test method | PCR |Specify: |
| | ELISA |Specify: |
| | Bioassay |Specify: |
| | |Specify: |
Additional information on the test method.
| |
| |
| |
Date and signature
| |11th ISTA GMO Proficiency Test, Canola/Oil Seed Rape (Brassica sp.) |
|Laboratory Name: | |Laboratory No.: |
| | | |
2.B. Quantitative Test
2.B.1. Quantitative Results
|Sample # |1 Test result |2 Test result |3 Test result |
| |% of GM seeds in # of seeds |% of GM seeds in mass of |% # of DNA copies |
| | |seeds | |
| | | |Please note that results |
| | | |reported in this column will be|
| | | |evaluated against the median of|
| | | |all results reported as %# DNA |
| | | |copies for one spiking level |
| | Total RF3, T45 | |
|1 | | | |
|2 | | | |
|3 | | | |
|4 | | | |
|5 | | | |
|6 | | | |
|7 | | | |
|8 | | | |
|9 | | | |
|10 | | | |
|11 | | | |
|12 | | | |
2.B.2. Optional information: method used for the quantitative test
|Reference material used: | |
|Type of test method | PCR |Specify: |
|Limit of detection (LOD): | | ELISA |Specify: |
|Limit of quantification (LOQ): | | Bioassay |Specify: |
Any additional information on the test method or how the LOD and the LOQ are obtained may be provided on separate sheets.
| |
Date and signature
| |11th ISTA GMO Proficiency Test, Canola/Oil Seed Rape (Brassica sp.) |
|Laboratory Name: | |Laboratory No.: |
| | | |
2.C Raw Quantitative Results
Use the table below as an example on how to submit raw quantitative data related to the test results. Please add according to your testing design the sample numbers, flour sub-sample numbers, and measurement numbers (replicates) in an Excel sheet. Please respect the column order. It is very important that the ISTA sample number of the sample label is reported in the first column.
Example: Sample number 1 to 3 are divided into two flour sub-sample. Each sub-sample is measured three times.
|Unit | |
|sample # |flour sub-sample |measurement |test results |
| |# |# (Replicates) | |
|1 |1 |1 | |
|1 |1 |2 | |
|1 |1 |3 | |
|1 |2 |1 | |
|1 |2 |2 | |
|1 |2 |3 | |
|2 |1 |1 | |
|2 |1 |2 | |
|2 |1 |3 | |
|2 |2 |1 | |
|2 |2 |2 | |
|2 |2 |3 | |
|3 |1 |1 | |
|3 |1 |2 | |
|3 |1 |3 | |
|3 |2 |1 | |
|3 |2 |2 | |
|3 |2 |3 | |
Please provide additional information on calibration at your own discretion.
| |11th ISTA GMO Proficiency Test, Canola/Oil Seed Rape (Brassica sp.) |
|Laboratory Name: | |Laboratory No.: |
| | | |
3. Identification and event specific Quantification
This part is optional and will not be included in the rating.
3.1. Identification Results Quantification Results
| |(please tick as appropriate) |Unit used : |
|Sample |RF3 |T45 |RF3 |T45 |Additional remarks |
|1 | | | | | |
|2 | | | | | |
|3 | | | | | |
|4 | | | | | |
|5 | | | | | |
|6 | | | | | |
|7 | | | | | |
|8 | | | | | |
|9 | | | | | |
|10 | | | | | |
|11 | | | | | |
|12 | | | | | |
3.2. Optional Information: method used for identification test
|Type of test method | PCR |Specify: |
| | ELISA |Specify: |
| | Bioassay |Specify: |
| | derived from the quantitative test result |
Please provide additional information about the test method (e.g. grinding method; DNA extraction method; target for PCR, ELISA or bioassay; primer sequences; test kit; method publication/validation references etc.).
| |
| |
| |
| |
Date and signature
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