Agilent 6460 Triple Quad Mass Spectrometer (Model K6460 ...

Agilent 6460 Triple Quad Mass Spectrometer (Model K6460) System

Concepts Guide

The Big Picture

For In Vitro Diagnostic Use

Agilent Technologies

Notices

? Agilent Technologies, Inc. 2016

No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.

Manual Part Number

K3335-90205

Edition

Revision A, August 2016

Printed in USA

Agilent Technologies, Inc. 5301 Stevens Creek Blvd. Santa Clara, CA 95051

Software Revision

This guide applies to the Agilent MassHunter Workstation Software -- Data Acquisition for 6460 Triple Quadrupole MS program version B.08.00.

Notice to Customer

The K6460 mass spectrometer is intended to be used to identify inorganic or organic compounds in human specimens by ionizing the compounds and separating the resulting ions by means of electrical field according to their mass.

Warranty

The material contained in this document is provided "as is," and is subject to being changed, without notice, in future editions. Further, to the maximum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a particular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or performance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the separate agreement shall control.

Technology Licenses

The hardware and/or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license.

Restricted Rights Legend

U.S. Government Restricted Rights. Software and technical data rights granted to the federal government include only those rights customarily provided to end user customers. Agilent provides this customary commercial license in Software and technical data pursuant to FAR 12.211 (Technical Data) and 12.212 (Computer Software) and, for the Department of Defense, DFARS 252.227-7015 (Technical Data - Commercial Items) and DFARS 227.7202-3 (Rights in Commercial Computer Software or Computer Software Documentation).Safety Notices

CAUTION

A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.

WARNING

A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met.

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Agilent K6460 Triple Quad Mass Spectrometer Concepts

In This Guide...

The Concepts Guide presents "The Big Picture" behind the operation of the K6460 Triple Quad Mass Spectrometer by helping you understand how the hardware and software work.

1 Overview

Learn how the K6460 Triple Quad Mass Spectrometer helps you do your job.

2 Inner Workings

Learn how the K6460 Triple Quad Mass Spectrometer helps you do your job.

3 K6460 Triple Quad Mass Spectrometer and Sensitivity

Learn how the K6460 Triple Quad Mass Spectrometer achieves high sensitivity.

4 Agilent MassHunter Workstation Software - Data Acquisition for 6400 Series Triple Quadrupole

Learn concepts behind the design of the Agilent MassHunter Workstation Software - Data Acquisition for 6400 Series Triple Quadrupole Mass Spectrometer.

Agilent K6460 Triple Quad Mass Spectrometer Concepts Guide

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Agilent K6460 Triple Quad Mass Spectrometer Concepts

Content

1 Overview 7 Supported configuration 8 Help for acquisition 9 Help for data analysis 11

2 Inner Workings 13 Design of the K6460 Triple Quad Mass Spectrometer 13 Innovative Enhancements in the K6460 15 How a triple quadrupole mass spectrometer works 16 How Dynamic MRM works 18 How Triggered Dynamic MRM works 21

3 K6460 Triple Quad Mass Spectrometer and Sensitivity 27

How the K6460 Triple Quad Mass Spectrometer improves sensitivity 28 Noise reduction 28 Example of chemical noise reduction 30

.How each component works to improve sensitivity 32 Mass Spectrometer Ion Sources 32 Front-end ion optics 36 Collision cell 38 Detector 42 Pumping system 43

4 Agilent MassHunter Workstation Software - Data Acquisition for 6400 Series Triple Quadrupole 45 Tuning 47 Acquisition 50

Agilent K6460 Triple Quad Mass Spectrometer Concepts Guide

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Agilent K6460 Triple Quad Mass Spectrometer Concepts Guide

K6460 Triple Quad Mass Spectrometer Concepts Guide

1 Overview

Supported configuration 8 Help for acquisition 9 Help for data analysis 11

This chapter provides an overview of the K6460 Triple Quad Mass Spectrometer components and how they help get the job done.

Agilent Technologies

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1 Overview Supported configuration

Supported configuration

ESI ? Electrospray Ionization APCI ? Atmospheric Pressure

Chemical Ionization AJS ? Agilent Jet Stream

Technology

The K6460 Triple Quad Mass Spectrometer can be used with a K1260 LC stack. The supported ion sources are AJS, ESI, and APCI.

The AJS source uses a super-heated sheath gas to collimate the nebulizer spray, which dramatically increases the number of ions that enter the mass spectrometer.

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Agilent K6460 Triple Quad Mass Spectrometer Concepts Guide

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