Based on the gene-expression profiles in samples of ...
Supplementary Data:
[pic]
Figure S1. Related to Figure 1.
(A) Schematic representation of in vitro loss-of-function screen used to identify C/EBPα downstream genes that confer enhanced self-renewal properties. Sorted LSK cells from Cebpa KO mice were transduced with a pool of lentiviruses carrying a puromycin resistance gene as well as scrambled shRNA or shRNA targeting specific genes. Serial replating assays were performed every 10 days. Both colony number and cell differentiation was assessed for each round.
(B) qPCR analysis of Sox4 expression in cells harvested from the first and second plating round in Figure 1B. Relative Sox4 expression levels were determined as % Gapdh and represented the mean ± SEM of 3 independent experiments. *: p ................
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