INTRODUCTION - Susquehanna University

You will treat the cover slips with the secondary (2°) antibody - a TRITC-conjugated anti-mouse antibody. The 2° ab staining solution also contains FITC -phalloidin, which will bind to and stain F-actin green, and DAPI to stain the DNA in the cell blue. Finally, the cover slips will be mounted with Prolong Antifade mounting solution. 1. ................
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