BIOLOGY 105: Study Questions for Exam 2 (Sebastian Kadener)
How were the Pol II GTFs first identified? Fractionation of nuclear extracts to find GTFs Fractionation scheme, DEAE cellulose. What is a TATA-box? TATA box found about 20-25 bp 5’ to the start site for transcription of many (but not all) genes transcribed by RNA polymerase II. How is it recognized? What are TAF's? TBP bends the DNA about 90 ... ................
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