Normal.dot - Osteoarthritis
Fmoc-O2Oc-OH was coupled as a spacer to peptides subjected to FITC modification. FITC-labelling was performed twice with a 4-fold excess of FITC Isomer I and 8 eq. DIPEA in NMP for 1 h. Peptides were cleaved from the resin with TFA/H2O/TIPS (95/2.5/2.5) for 4 h and precipitated with diethyl ether at -20_C. Semi preparative HPLC was carried out ... ................
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