Pineapple Enzyme Formal Lab Report



Lab 5 Pineapple Enzymes

Formal Lab Report Guidelines

General: Size 12 font, Times New Roman, 1 inch margins, double spaced. Label each section of report (with the exception of title).

The Lab Report

1. Title

2. Abstract

3. Introduction

4. Materials and Methods

5. Results

6. Discussion

7. Sources of Error

8. Literature Cites

1. Title: Both number and name, at the top of the page, centered. Use a specific title that cues reader into content of experiment.

2. Abstract: The purpose of an abstract is to allow the reader to judge whether it would serve his or her purposes to read the entire report. Keep the length generally between 50-75 words. To help you, answer the questions below to make sure you cover everything.

- What was the purpose of this lab? DO NOT say something vague like “To see what effect Bromelin has on Jell-O”. Be specific. There were two purposes because there were two parts of the lab.

- What fruit test tubes solidified? Which heated test tubes solidified (Give Data).

- Which fruits had the enzyme Bromelain? At or beyond which temperature does Bromelain appear to denature? (General conclusions).

3. Introduction: The introduction defines the subject of the report, outlining what knowledge already exists about the subject. It must give the reader sufficient background to understand the rest of the report. It should include information collected from resources such as articles, texts, and reliable Internet sources. Include vocabulary from class whenever possible. To help you, answer the questions below to make sure you cover everything.

- What is bromelain? What reaction does it work on?

- Generally speaking, what is an enzyme?

- How does an enzyme work?

- What is denaturation of an enzyme? How does it prevent an enzyme from working?

4. Materials and Methods: As the name implies, the materials and methods used in the experiments should be reported in this section. The difficulty in writing this section is to provide enough detail for the reader to understand the experiment without overwhelming him or her. One should describe special pieces of equipment. This can usually be done in a short paragraph, possibly along with a drawing of the experimental apparatus. Do not list the materials or procedure, but rather write in paragraph form. Write in past tense (because

- What did you do in lab? Be specific. Use quantities; give specific temperatures, time lengths, etc. Speak in past tense.

5. Results: The results section should summarize the data from the experiments without discussing their implications. The data should be organized into tables, figures, graphs, photographs, and so on. All figures and tables should have descriptive titles and should be numbered separately.

- Copy and paste the class’s data chart into this section.

- Underneath this, title it “Figure 1. Lab 5 Results”

6. Discussion: This section should not just be a restatement of the results but should emphasize interpretation of the data, relating them to existing theory and knowledge. In writing this section, you should explain the logic that allows you to accept or reject your original hypotheses. To help you, answer the questions below to make sure you cover everything.

- What does the results from your negative and positive control illustrate? Is the enzyme/jello working as expected?

- Which fruits have the enzyme? How do you know?

- At which temperature does Bromelain denature? How do you know?

7. Sources of Error: How could we have done this experiment differently to improve the validity of our results? Where were their potential errors in the experiment? Do not include errors that could have been avoided (i.e. “human error” like measuring, etc).

- Suggest one thing that could we have done to improve this lab

8. Sources Cited: Begin on new page. Single spaced. List any source from text or credible internet source alphabetically using format below, but do not number.

For articles:
 
Author Last Name, First Name. Publishing Year. Name of Article. Name of Journal. Journal Number: Page Numbers.

For Books/Texts: 
Author Last Name, First Name. Publishing Year. Name of Text. City of Printing: Publishing Company.

For Internet Source: Title of the Site. Editor. Last updated Date and/or Version Number. Name of Sponsoring Institution. Date of Access .

For the Lab Sheet: Franczyk, Kata “Lab 5: Pineapple Enzymes Lab”. 2011. Kingswood Oxford School.

When citing references in the text, do not use footnotes; instead, refer to articles by the author's name and the date the paper was published. For example:

Correct - Fox in 1988 investigated the hormones on the nest- building behavior of catbirds.

Incorrect - Hormones are known to influence the nest-building behavior of catbirds (Fox, 1988).

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