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|Molecular (PCR) |CHLAMYDIA TRACHOMATIS AND GONOCOCCI |Aptima Urine Sample |
|detection of GC/CT |PCR |The patient should not have urinated for at least 1 hour prior to specimen collection. |
| | |Direct patient to provide a first-catch urine (approximately 20 to 30 mL of the initial urine stream) into a urine collection cup free of any |
| |EYE SWAB CHLAMYDIA PCR |preservatives. (Collection of larger volumes of urine may result in rRNA target dilution that may reduce test sensitivity). |
| | |Female patients should not cleanse the labial area prior to providing the specimen. |
| |CHLAMYDIA PCR MEDICO LEGAL |Remove the cap and transfer 2 mL of urine into the urine specimen transport tube using the disposable pipette provided. The correct volume of urine|
| | |has been added when the fluid level is between the black fill lines on the urine specimen transport tube label. 4. Re-cap the urine specimen |
| | |transport tube tightly. |
| | | |
| | |Aptima vaginal swabs (orange label) |
| | |Partially peel open the swab package. Remove the swab. Do not touch the soft tip or lay the swab down. If the soft tip is touched, the swab is laid|
| | |down, or the swab is dropped, use a new APTIMA Vaginal Swab Specimen Collection Kit. |
| | |Hold the swab, placing your thumb and forefinger in the middle of the swab shaft covering the score line. Do not hold the swab shaft below the |
| | |score line. |
| | |Carefully insert the swab into the vagina about 2 inches (5 cm) past the introitus and gently rotate the swab for 10 to 30 seconds. Make sure the |
| | |swab touches the walls of the vagina so that moisture is absorbed by the swab and then withdraw the swab without touching the skin. |
| | |While holding the swab in the same hand, unscrew the cap from the tube. Do not spill the contents of the tube. If the contents of the tube are |
| | |spilled, use a new APTIMA Vaginal Swab Specimen Collection Kit. |
| | |Immediately place the swab into the transport tube so that the score line is at the top of the tube. |
| | |Carefully break the swab shaft at the score line against the side of the tube. |
| | |Immediately discard the top portion of the swab shaft. |
| | |Tightly screw the cap onto the tube. |
| | | |
| | |Aptima endocervical swabs (white and purple label) |
| | |Remove excess mucus from the cervical os and surrounding mucosa using the cleaning swab (white shaft swab in the package with red printing). |
| | |Discard this swab. Note: To remove excess mucus from the cervical os, a large-tipped swab (not provided) may be used. |
| | |Insert the specimen collection swab (blue shaft swab in the package with the green printing) into the endocervical canal. Gently rotate the swab |
| | |clockwise for 10 to 30 seconds in the endocervical canal to ensure adequate sampling. |
| | |Withdraw the swab carefully; avoid any contact with the vaginal mucosa. |
| | |Remove the cap from the swab specimen transport tube and immediately place the specimen collection swab into the transport tube. |
| | |Carefully break the swab shaft against the side of the tube at the scoreline and discard the top portion of the swab shaft; use care to avoid |
| | |splashing of contents. |
| | |Re-cap the swab specimen transport tube tightly. |
| | | |
| | |Aptima male urethral swabs (white and purple label) |
| | |The patient should not have urinated for at least 1 hour prior to sample collection. |
| | |Insert the specimen collection swab (blue shaft swab in the package with the green printing) 2 to 4 cm into the urethra. |
| | |Gently rotate the swab clockwise for 2 to 3 seconds in the urethra to ensure adequate sampling. |
| | |Withdraw the swab carefully. |
| | |Remove the cap from the swab specimen transport tube and immediately place the specimen collection swab into the transport tube. |
| | |Carefully break the swab shaft against the side of the tube at the scoreline and discard the top portion of the swab shaft; use care to avoid |
| | |splashing of contents. |
| | |Re-cap the swab specimen transport tube tightly. |
| | | |
| | |Aptima eye swabs (orange label) |
| | |Partially peel open the swab package. Remove the swab. Do not touch the soft tip or lay the swab down. If the soft tip is touched, the swab is laid|
| | |down, or the swab is dropped, use a new APTIMA Swab Specimen Collection Kit. |
| | |Hold the swab, placing your thumb and forefinger in the middle of the swab shaft covering the score line. Do not hold the swab shaft below the |
| | |score line. |
| | |Hold the swab parallel to the cornea and gently rub the conjunctiva in the lower eyelids from nasal side outwards. |
| | |Swab any pus or exudates as well as any lesion of interest. |
| | |While holding the swab in the same hand, unscrew the cap from the tube. Do not spill the contents of the tube. If the contents of the tube are |
| | |spilled, use a new APTIMA Vaginal Swab Specimen Collection Kit. |
| | |Immediately place the swab into the transport tube so that the score line is at the top of the tube. |
| | |Carefully break the swab shaft at the score line against the side of the tube. |
| | |Immediately discard the top portion of the swab shaft. |
| | |Tightly screw the cap onto the tube. |
| | |If both eyes are to be swabbed, label swabs ‘right’ and ‘left’ accordingly |
|Rectal swab for |LYMPHOGRANULOMA VENEREUM (LGV) PCR |Aptima rectal swabs (white and purple label) |
|lymphogranuloma venereum| |Partially peel open the swab package. Remove the swab. Do not touch the soft tip or lay the swab down. If the soft tip is touched, the swab is laid|
|(LGV) | |down, or the swab is dropped, use a new APTIMA Swab Specimen Collection Kit. |
| | |Hold the swab, placing your thumb and forefinger in the middle of the swab shaft covering the score line. Do not hold the swab shaft below the |
| | |score line. |
| | |Carefully pass the swab through the anus into the rectum and rotate gently. |
| | |Withdraw the swab carefully. |
| | |Remove the cap from the swab specimen transport tube and immediately place the specimen collection swab into the transport tube. |
| | |Carefully break the swab shaft against the side of the tube at the scoreline and discard the top portion of the swab shaft; use care to avoid |
| | |splashing of contents. |
| | |Re-cap the swab specimen transport tube tightly. |
|Nasopharyngeal Aspirate |MERS-COV CORONAVIRUS REAL-TIME PCR |For general aspects of taking respiratory samples we recommend that users follow either their local protocols or “The Royal Marsden Manual of |
|(NPA) | |Clinical Nursing Procedures”. |
| |HHV6 PCR (QUALITATIVE AND | |
| |QUANTITATIVE) |Nasopharyngeal Aspirate (NPA) |
| | |NPA should be collected according to local protocols using sterile fluids as the instillate. |
| |INFLUENZA A H7N9 REAL-TIME PCR |Recovered mucus/fluid should sent in a 20ml white top sterile sample container or other well sealed sterile container. |
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| |RESPIRATORY VIRUSES PCR | |
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| |REAL-TIME PCR DETECTION OF BORDETELLA| |
| |PERTUSSIS | |
|Nose and Throat Swabs |MERS-COV CORONAVIRUS REAL-TIME PCR |Nose and throat swabs for virology |
|for Virology | |Use a red topped virology transport medium kit (labelled as Oxoid IDEIA Chlamydia kit). |
| |INFLUENZA A H7N9 REAL-TIME PCR |Throat: Ask patient to sit upright facing a strong light, tilt head backwards, open mouth and stick out tongue. Depress tongue with a spatula. Ask |
| | |patient to say ‘Ah’. Quickly but gently roll the swab over the tonsils and posterior pharynx |
| |MEASLES VIRUS PCR |Nose: Ask patient to tilt head backwards. Insert swab inside the anterior nares with the tip directed upwards and gently rotate. |
| | |Place the swab(s) in the transport liquid and snap off the swab shaft at the score line so it fits into the sample pot. |
| |RESPIRATORY VIRUSES PCR |Close the cap tightly and label with patient information. |
| | |Label as “nose”, “throat” or “nose and throat” |
| |HHV6 PCR (QUALITATIVE AND | |
| |QUANTITATIVE) |Throat swabs only for REAL-TIME PCR DETECTION OF BORDETELLA PERTUSSIS and MYCOPLASMA PNEUMONIAE REAL-TIME PCR (Take out nose stuff for these |
| | |tests): |
| |INFLUENZA A H7N9 REAL-TIME PCR | |
| | |Throat swabs for virology |
| |INFLUENZA A CONFIRMATORY TYPING |Use a red topped virology transport medium kit (labelled as Oxoid IDEIA Chlamydia kit). |
| | |Ask patient to sit upright facing a strong light, tilt head backwards, open mouth and stick out tongue. Depress tongue with a spatula. Ask patient |
| |REAL-TIME PCR DETECTION OF BORDETELLA |to say ‘Ah’. Quickly but gently roll the swab over the tonsils and posterior pharynx |
| |PERTUSSIS |Place the swab in the transport liquid and snap off the swab shaft at the score line so it fits into the sample pot. |
| | |Close the cap tightly and label with patient information. |
| |MYCOPLASMA PNEUMONIAE REAL-TIME PCR | |
|Genital swab for |VIRAL GENITAL SWAB SCREEN |Genital swab for virology |
|virology | |Use a red topped virology transport medium kit (labelled as Oxoid IDEIA Chlamydia kit). |
| |GENITAL ULCERATIVE DISEASE SCREEN |Rotate swab over surface of ulcer collecting any exudate. |
| |(GUD) |Place the swab in the transport liquid and snap off the swab shaft at the score line so it fits into the sample pot. |
| | |Close the cap tightly and label with patient information. |
|Skin swab for virology |SKIN LESION- VIRAL SCREEN |Skin swab for virology |
| | |Use a red topped virology transport medium kit (labelled as Oxoid IDEIA Chlamydia kit). |
| | |Rotate swab over surface of ulcer/vesicle collecting any exudate. |
| | |Place the swab in the transport liquid and snap off the swab shaft at the score line so it fits into the sample pot. |
| | |Close the cap tightly and label with patient information. |
|Eye swab for virology |VIRAL EYE SCREEN PCR |Eye swab for virology |
| | |Use a red topped virology transport medium kit (labelled as Oxoid IDEIA Chlamydia kit). |
| | |Swab parallel to the cornea and gently rub the conjunctiva in the lower eyelids from nasal side outwards. |
| | |Swab any pus or exudates as well as any lesion of interest. |
| | |Place the swab in the transport liquid and snap off the swab shaft at the score line so it fits into the sample pot. |
| | |Close the cap tightly and label with patient information. |
| | |If both eyes are to be swabbed, label swabs ‘right’ and ‘left’ accordingly. |
|Mouth swab for virology |VIRAL MOUTH SWAB SCREEN |Mouth swab for virology |
| | |Use a red topped virology transport medium kit (labelled as Oxoid IDEIA Chlamydia kit). |
| | |Swab lesion of interest. |
| | |Place the swab in the transport liquid and snap off the swab shaft at the score line so it fits into the sample pot. |
| | |Close the cap tightly and label with patient information. |
| | |If both eyes are to be swabbed, label swabs ‘right’ and ‘left’ accordingly. |
|Blood Spots |DRIED BLOOD SPOT TESTING |Click on hyperlink for Dried Blood Spot Testing to take you to the DBS entry on the test database. Please refer to links on this page for detailed |
| | |information on how to take dried blood spot samples |
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|EDTA whole blood and/or |ADENOVIRUS VIRAL LOAD |For taking blood samples we recommend that users follow either their local protocols or “The Royal Marsden Manual of Clinical Nursing Procedures”. |
|plasma | | |
| |CYTOMEGALOVIRUS (CMV) UL97 GENOTYPIC |Lavender top blood (EDTA) samples |
| |RESISTANCE |Sufficient blood for the test should be drawn into lavender topped (EDTA) vacuum tubes. |
| | |Invert tube 8–10 times. |
| |CYTOMEGALOVIRUS VIRAL (CMV) LOAD | |
| | |Specific additional instructions for CCR5 tropism already on webpage. |
| |EPSTEIN BARR VIRUS VIRAL LOAD | |
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| |ERYTHROVIRUS (PARVOVIRUS B19 )PCR | |
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| |HEPATITIS B GENOTYPIC RESISTANCE | |
| |TESTING | |
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| |HEPATITIS B VIRAL LOAD | |
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| |HEPATITIS C GENOTYPING | |
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| |HEPATITIS C VIRAL LOAD | |
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| |HEPATITIS E PCR | |
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| |HIV 1 GENOTYPIC RESISTANCE TESTING | |
| |(PROTEASE AND REVERSE TRANSCRIPTASE | |
| |REGION) | |
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| |HIV 1 INTEGRASE GENOTYPIC RESISTANCE | |
| |TESTING | |
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| |HIV GP41 - T20 GENOTYPIC RESISTANCE | |
| |TESTING | |
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| |HIV VIRAL LOAD | |
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| |HIV2 GENOTYPIC RESISTANCE TESTING | |
| |(PROTEASE AND REVERSE TRANSCRIPTASE | |
| |REGION) | |
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| |POLYOMAVIRUS VIRAL LOAD | |
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| |HEPATITIS C IGG CONFIRMATORY | |
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| |HEPATITIS C IGG SCREEN | |
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| |HEPATITIS E IGM/IGG ANTIBODY | |
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| |HHV6 PCR (QUALITATIVE AND | |
| |QUANTITATIVE) | |
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| |PNEUMOCYSTIS JIROVECII REAL-TIME PCR | |
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| |HIV CCR5 TROPISM | |
|Red top blood (Serum) |CYTOMEGALOVIRUS (CMV) IGG |For taking blood samples we recommend that users follow either their local protocols or “The Royal Marsden Manual of |
|NOT INCLUDING antibiotic| |Clinical Nursing Procedures”. |
|level assays | | |
| |CYTOMEGALOVIRUS (CMV) IGM |Red top blood (serum) samples |
| | |Sufficient blood for the test should be drawn into red topped (no additive) vacuum tubes. |
| |EPSTEIN BARR VIRUS (EBV) ANTIBODY | |
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| |ERYTHROVIRUS (PARVOVIRUSB19) IGM/IGG | |
| |ANTIBODY | |
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| |HELICOBACTER IGG | |
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| |HEPATITIS A IGG | |
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| |HEPATITIS A IGM | |
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| |HEPATITIS B CORE ANTIBODY | |
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| |HEPATITIS B CORE IGM ANTIBODY | |
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| |HEPATITIS B E ANTIBODY | |
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| |HEPATITIS B E ANTIGEN | |
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| |HEPATITIS B SURFACE ANTIBODY - POST | |
| |VACCINATION | |
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| |HEPATITIS B SURFACE ANTIGEN | |
| |CONFIRMATORY | |
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| |HEPATITIS B SURFACE ANTIGEN SCREEN | |
| |HERPES 1/2 TOTAL IGG ANTIBODY | |
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| |HIV 1/2 ANTIGEN/ANTIBODY CONFIRMATORY | |
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| |HIV 1/2 ANTIGEN/ANTIBODY SCREEN | |
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| |HIV P24 ANTIGEN | |
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| |MEASLES IGG ANTIBODY | |
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| |MEASLES IGM | |
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| |MUMPS IGG ANTIBODY | |
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| |MUMPS IGM | |
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| |RUBELLA IGG | |
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| |RUBELLA IGM | |
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| |STREPTOCOCCUS TESTING (ASOT) | |
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| |SYPHILIS CONFIRMATORY | |
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| |SYPHILIS SCREEN | |
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| |VARICELLA ZOSTER IGG ANTIBODY | |
| |VARICELLA ZOSTER IGM ANTIBODY | |
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| |BORRELIA BURGDORFERI (LYME) ANTIBODY | |
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| |HEPATITIS C IGG CONFIRMATORY | |
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| |HEPATITIS C IGG SCREEN | |
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| |HEPATITIS E IGM/IGG ANTIBODY | |
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| |CRYPTOCOCCUS ANTIGEN | |
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