GenScript - Protein Services
Customer Information
If you have registered an account with GenScript, you can just identify yourself by giving us your name and email address or Account No.
|Name: | |
|Account No.: | |
|Phone: | |
|Organization: | |
|Shipping address: | |
|(Necessary to determine shipping | |
|cost) | |
|Email address: | |
Protein Expression
|Target protein: |Protein name: Species: MW: |
| |Protein sequence: (crucial for evaluation) or accession number: |
| |Note: Please enter the protein sequence if mutants are included in your protein sequence. |
|Starting material: | Gene synthesis with codon optimization (For Human/Mouse/Rat ORF genes exist in NCBI database, starting from $0.29/bp; |
| |for other genes, starting from $0.39/bp) |
| |(Please enter your gene sequence: ) |
| |Gene synthesis without codon optimization (For Human/Mouse/Rat ORF genes exist in NCBI database, starting from $0.29/bp; |
| |for other genes, starting from $0.39/bp) |
| |(Please enter your gene sequence: ) |
| |Your supplied template DNA |
| |(Please enter your gene sequence: , vector name: and attach vector map (e.g. NTI file) |
| |Your supplied expression ready construct |
| |(Please enter your vector name: , vector sequence: , and attach vector map (e.g. NTI file, please indicate your|
| |cloning site) |
| |Others (Please specify): |
|Expression system: |Please indicate the preferred expression host system: |
| |Bacteria Yeast Baculovirus/insect cell Mammalian cell |
| |If mammalian cell expression system is preferred, please select your preferred host cell line: |
| |HEK293 CHO Others (Please specify): No preference |
| |Notes: If you DO NOT have preference, HEK293 or its derivatives will be used as default cell line in our platform. |
| | |
| |Has the gene been expressed in any host before? |
| |Yes No |
| |If YES, please indicate the host: |
| |Bacteria Yeast Baculovirus/insect cell Mammalian cell |
| |Others (Please specify): |
| |Yield: Solubility: Protocol: Other information: |
| |Notes: The above information is crucial for us to evaluate your project. |
|Secretion signal sequence: (For |Do you have any preferred secretion signal sequence for expressing your protein of interest? |
|insect cell and mammalian cell |Its own signal peptide No preference Others (Please specify): |
|expression systems) | |
| |Please specify your preferred expression format: |
| |Secretory expression only (GenScript will only purify the protein from the medium) |
| |Protein purification from the cell lysate if very little protein can be obtained from the medium (Extra fee is charged) |
| |Expression evaluations on both secretory expression with the signal peptide and intracellular expression without the |
| |signal peptide (Recommended) |
|Expression scale: |Amount of protein: mg or Volume of culture required: liter(s) |
|Protein properties: | Membrane-bound Secreted A protease Other enzyme |
| |Cytoplasmic protein IgG antibody Toxic to E.coli |
| |Other features that may cause difficulty in either expression or purification (Please specify): |
|Application(s) of your protein: | High-throughput screening (HTS) Activity assays NMR X-ray crystallography |
|(Please select all that apply) |Antigen in vivo studies |
| |Protein-protein interactions Others (Please specify): |
|Comments: | |
Protein Purification
|Product requirements: |Purity: % Endotoxin Limit: Concentration: |
|Purification method: |Do you want GenScript to recommend a purification system for purifying your protein of interest? |
| |Yes No, I want to choose my own purification system (Please specify): |
| | |
| |Do you need GenScript to follow any specific expression and/or purification protocol? |
| |Yes (Please attach your protocol: ) No |
| |Note: Your protocol will be very helpful to increase success rate and shorten the delivery time. |
|Tag(s) selection: |Do you want GenScript to select 1 or 2 tags (6xHis, GST, or MBP) to facilitate purification? |
| |Yes No, I want to choose my own tag(s) (Please specify): |
| | |
| |Do you want GenScript to select additional 1 or 2 tags (Trx, TF, Dsb, or Nus) to increase solubility and/or expression |
| |level? (Recommended—can greatly improve yield; the tag(s) will be added to N-terminus) |
| |Yes No, I want to choose my own tag(s) (Please specify): |
| | |
| |Do you want GenScript to add the tag(s) at the N-terminus? |
| |Yes No, I have designated place(s) to add the tag(s) (Please specify): |
| |Note: If internal tag(s) is/are involved, please designate the position number(s) that corresponds to the sequence of |
| |which the accession number you have indicated above. |
|Tag(s) removal: |Do you want GenScript to add a protease cleavage site between the tags and the target protein? |
| |Yes No |
| |If YES, please specify the protease you want to add: |
| |GenScript’s choice Enterokinase PreScission rTev Thrombin |
| |Note: An extra of 4-6 aa will be left after protease cleavage of C-terminal tag, therefore, we DO NOT recommend the |
| |introduction of protease cleavage site between the target protein and the C-terminal tag. |
| | |
| |Do you want GenScript to remove tag(s) in the purification step? |
| |Yes No Small tag (e.g. His) can be retained after purification |
| |Note: The recovery rate varies with the characteristics of the protein. |
|Refolding: |If the expressed protein is insoluble, do you want GenScript to attempt the refolding? |
| |Yes No |
| |Note: GenScript will NOT charge extra fee for conventional refolding. |
|Protein characterization: | SDS-PAGE IEF ELISA HPLC Endotoxin |
| |N-terminal sequence MALDI-TOF Others (Please specify): |
|Special requirements: (information| |
|on buffers or handling, etc.) | |
|Comments: | |
Project Information
|Nature of inquiry: | Pricing estimation Quote for ordering For grant application purpose |
|Order initiation: | Immediately Within one month Within three months |
| |Within six months |
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Protein Expression and Purification Service Quotation Request Form
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