GenScript - Protein Services



Customer Information

If you have registered an account with GenScript, you can just identify yourself by giving us your name and email address or Account No.

|Name: |           |

|Account No.: |      |

|Phone: |      |

|Organization: |      |

|Shipping address: |      |

|(Necessary to determine shipping | |

|cost) | |

|Email address: |      |

Protein Expression

|Target protein: |Protein name:       Species:       MW:       |

| |Protein sequence: (crucial for evaluation)       or accession number:       |

| |Note: Please enter the protein sequence if mutants are included in your protein sequence. |

|Starting material: | Gene synthesis with codon optimization (For Human/Mouse/Rat ORF genes exist in NCBI database, starting from $0.29/bp; |

| |for other genes, starting from $0.39/bp) |

| |(Please enter your gene sequence:      ) |

| |Gene synthesis without codon optimization (For Human/Mouse/Rat ORF genes exist in NCBI database, starting from $0.29/bp; |

| |for other genes, starting from $0.39/bp) |

| |(Please enter your gene sequence:      ) |

| |Your supplied template DNA |

| |(Please enter your gene sequence:      , vector name:       and attach vector map (e.g. NTI file) |

| |Your supplied expression ready construct |

| |(Please enter your vector name:      , vector sequence:      , and attach vector map (e.g. NTI file, please indicate your|

| |cloning site) |

| |Others (Please specify):       |

|Expression system: |Please indicate the preferred expression host system: |

| |Bacteria Yeast Baculovirus/insect cell Mammalian cell |

| |If mammalian cell expression system is preferred, please select your preferred host cell line: |

| |HEK293 CHO Others (Please specify):       No preference |

| |Notes: If you DO NOT have preference, HEK293 or its derivatives will be used as default cell line in our platform. |

| | |

| |Has the gene been expressed in any host before? |

| |Yes No |

| |If YES, please indicate the host: |

| |Bacteria Yeast Baculovirus/insect cell Mammalian cell |

| |Others (Please specify):            |

| |Yield:       Solubility:       Protocol:       Other information:       |

| |Notes: The above information is crucial for us to evaluate your project. |

|Secretion signal sequence: (For |Do you have any preferred secretion signal sequence for expressing your protein of interest? |

|insect cell and mammalian cell |Its own signal peptide No preference Others (Please specify):       |

|expression systems) | |

| |Please specify your preferred expression format: |

| |Secretory expression only (GenScript will only purify the protein from the medium) |

| |Protein purification from the cell lysate if very little protein can be obtained from the medium (Extra fee is charged) |

| |Expression evaluations on both secretory expression with the signal peptide and intracellular expression without the |

| |signal peptide (Recommended) |

|Expression scale: |Amount of protein:       mg or Volume of culture required:       liter(s) |

|Protein properties: | Membrane-bound Secreted A protease Other enzyme |

| |Cytoplasmic protein IgG antibody Toxic to E.coli |

| |Other features that may cause difficulty in either expression or purification (Please specify):       |

|Application(s) of your protein: | High-throughput screening (HTS) Activity assays NMR X-ray crystallography |

|(Please select all that apply) |Antigen in vivo studies |

| |Protein-protein interactions Others (Please specify):            |

|Comments: |      |

Protein Purification

|Product requirements: |Purity:      % Endotoxin Limit:       Concentration:       |

|Purification method: |Do you want GenScript to recommend a purification system for purifying your protein of interest? |

| |Yes No, I want to choose my own purification system (Please specify):       |

| | |

| |Do you need GenScript to follow any specific expression and/or purification protocol? |

| |Yes (Please attach your protocol:      ) No |

| |Note: Your protocol will be very helpful to increase success rate and shorten the delivery time. |

|Tag(s) selection: |Do you want GenScript to select 1 or 2 tags (6xHis, GST, or MBP) to facilitate purification? |

| |Yes No, I want to choose my own tag(s) (Please specify):       |

| | |

| |Do you want GenScript to select additional 1 or 2 tags (Trx, TF, Dsb, or Nus) to increase solubility and/or expression |

| |level? (Recommended—can greatly improve yield; the tag(s) will be added to N-terminus) |

| |Yes No, I want to choose my own tag(s) (Please specify):       |

| | |

| |Do you want GenScript to add the tag(s) at the N-terminus? |

| |Yes No, I have designated place(s) to add the tag(s) (Please specify):       |

| |Note: If internal tag(s) is/are involved, please designate the position number(s) that corresponds to the sequence of |

| |which the accession number you have indicated above. |

|Tag(s) removal: |Do you want GenScript to add a protease cleavage site between the tags and the target protein? |

| |Yes No |

| |If YES, please specify the protease you want to add: |

| |GenScript’s choice Enterokinase PreScission rTev Thrombin |

| |Note: An extra of 4-6 aa will be left after protease cleavage of C-terminal tag, therefore, we DO NOT recommend the |

| |introduction of protease cleavage site between the target protein and the C-terminal tag. |

| | |

| |Do you want GenScript to remove tag(s) in the purification step? |

| |Yes No Small tag (e.g. His) can be retained after purification |

| |Note: The recovery rate varies with the characteristics of the protein. |

|Refolding: |If the expressed protein is insoluble, do you want GenScript to attempt the refolding? |

| |Yes No |

| |Note: GenScript will NOT charge extra fee for conventional refolding. |

|Protein characterization: | SDS-PAGE IEF ELISA HPLC Endotoxin |

| |N-terminal sequence MALDI-TOF Others (Please specify):       |

|Special requirements: (information|      |

|on buffers or handling, etc.) | |

|Comments: |      |

Project Information

|Nature of inquiry: | Pricing estimation Quote for ordering For grant application purpose |

|Order initiation: | Immediately Within one month Within three months |

| |Within six months |

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Protein Expression and Purification Service Quotation Request Form

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