PHOTOSYNTHESIS LAB BENCH VIRTUAL LAB



Name_____________________________________ Date ______________ Block: _______________

PHOTOSYNTHESIS LAB BENCH VIRTUAL LAB

Complete the following questions using the website:



Once you have navigated to the website, go through the screens by clicking the “next” button. You only need to read through 4-1 Chromatography.

1. What is paper chromatography?

2. What 3 factors affect the rate at which pigments migrate?

3. What is a pigment?

4. Which pigment went the farthest on the virtual ink Pigment Separation? Which pigment barely moved?

5. Name the 4 pigments from the plant separation in order from largest to smallest based on what you know about how far they travelled on the chromatography paper.

6. What is meant by the solvent front?

7. What is meant by the term Reference Front?

8. Imagine you actually completed the Section 4-1 of the Photosynthesis Lab, the Chromatography portion. Assume that the chromatogram drawn below is what you obtained after transferring the pigment onto the chromatography paper from a spinach leaf using a coin. Complete the following chart:

[pic]

What is the solvent front for the chromatogram drawn? ________

|Pigment Name |Colors Associated with this |Distance Pigment Migrated (mm) |Rf Value |

| |pigment | | |

|Chlorophyll a | | | |

|Chlorophyll b | | | |

|Carotene | | | |

|Xanthophyll | | | |

9. What are the two byproducts of photosynthesis that are important to humans

and other animals?

10. Name the pigments most common in spring and summer leaves.

11. Name the pigments most common in fall leaves.

Part 2

Procedure:

1. Place a small dot of ink approximately ½ inch from the bottom of the

chromatography paper.

2. Securely attach the chromatography paper onto a pencil and place in beaker.

3. Carefully pour the water down the side of the beaker until it touches the very

tip of the paper.

4. Allow the solvent to travel up the paper, separating out the pigments. Stop the

process when the solvent travels to within one inch of the top of the paper. Draw

a line at the solvent front before it evaporates.

5. Observe the various pigments from the ink sample and record results in table.

|Solute Color |Distance Solvent |Distance pigment |Rf= |

| |Traveled (cm) |Traveled (cm) |Dunknown/Dsolvent |

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1. What is the solvent used to separate the pigments in the ink?

2. Based upon the Rf values above, which pigment contains the smallest molecules?

3. What property allows water molecules to be drawn up through the filter paper?

Attach your chromatography paper to this sheet.

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