EDUCATION - Harvard University



L?LA FAGBAMI ofagbami@hsph.harvard.edu ? 617-843-5652 ? scholar.harvard.edu/lfagbamiEDUCATIONHarvard UniversityCambridge, MAGraduate School of Arts & ScienceChemical Biology (PhD)Aug 2014 – May 2019Marine Biological LaboratoriesWoods Hole, MASummer Discovery CoursesBiology of ParasitismJun 2016 – Aug 2016Rutgers UniversityStratford, NJSchool of Public HealthHealth Systems & Policy (MPH) Jan 2009 - Dec 2010Rutgers UniversityStratford, NJGraduate School of Biomedical ScienceBiomedical Science (MBS)Jan 2008 - May 2009Emory UniversityAtlanta, GACompleted in 3 yearsEmory CollegeChemistry (BS)Aug 2003 - Aug 2006 THESIS PROJECT: Chemogenomic Interrogation of the Adaptive Proline Response to Halofuginone in Plasmodium falciparum Advisors: Dyann Wirth (Harvard T.H. Chan School of Public Health) & Ralph Mazitschek (Massachusetts General Hospital)Treatment of Plasmodium falciparum parasites with halofuginone (HFG) triggers a novel mode of drug tolerance in which intracellular proline levels are increased by 30-fold prior to any alteration in the target cPRS gene. My thesis project investigated the mechanism of this adaptive proline response (APR) using a variety of technologies including mass-spectrometry based metabolomics, CRISPR/Cas9 genome editing, molecular cloning and biochemical assays. I discovered that arginine biosynthesis of proline enables the APR, while making novel observations in other aspects of parasite metabolism. My findings suggest possible combination therapies that can help prevent resistance to HFG-like antimalarials currently in development. Furthermore, this integrated approach can be used to illuminate the emergence of drug resistance for other classes of compounds and in other organisms.PUBLICATIONSFagbami, L., Deik, A. A., Singh, K., Santos, S. A., Herman, J. D., Bopp, S. E., Lukens, A. K., Clish, C. B., Wirth, D. F. & Mazitschek, R. The Adaptive Proline Response in P. falciparum Is Independent of PfeIK1 and eIF2α Signaling. ACS Infect Dis (2019). Rosenbluh, J., Mercer, J., Shrestha, Y., Oliver, R., Tamayo, P., Doench, J. G., Tirosh, I., Piccioni, F., Hartenian, E., Horn, H., Fagbami, L., Root, D. E., Jaffe, J., Lage, K., Boehm, J. S. & Hahn, W. C. Genetic and Proteomic Interrogation of Lower Confidence Candidate Genes Reveals Signaling Networks in β-Catenin-Active Cancers. Cell Syst 3, 302-316.e4 (2016).Ippoliti, P. J., Kuhn, E., Mani, D. R., Fagbami, L., Keshishian, H., Burgess, M. W., Jaffe, J. D. & Carr, S. A. Automated Microchromatography Enables Multiplexing of Immunoaffinity Enrichment of Peptides to Greater than 150 for Targeted MS-Based Assays. Anal. Chem. 88, 7548–7555 (2016).Abelin, J. G., Patel, J., Lu, X., Feeney, C. M., Fagbami, L., Creech, A. L., Hu, R., Lam, D., Davison, D., Pino, L., Qiao, J. W., Kuhn, E., Officer, A., Li, J., Abbatiello, S., Subramanian, A., Sidman, R., Snyder, E., Carr, S. A. & Jaffe, J. D. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Mol. Cell Proteomics 15, 1622–1641 (2016).Begun, J., Lassen, K. G., Jijon, H. B., Baxt, L. A., Goel, G., Heath, R. J., Ng, A., Tam, J. M., Kuo, S.-Y., Villablanca, E. J., Fagbami, L., Oosting, M., Kumar, V., Schenone, M., Carr, S. A., Joosten, L. A. B., Vyas, J. M., Daly, M. J., Netea, M. G., Brown, G. D., Wijmenga, C. & Xavier, R. J. Integrated Genomics of Crohn’s Disease Risk Variant Identifies a Role for CLEC12A in Antibacterial Autophagy. Cell Rep 11, 1905–1918 (2015).Lassen, K. G., Kuballa, P., Conway, K. L., Patel, K. K., Becker, C. E., Peloquin, J. M., Villablanca, E. J., Norman, J. M., Liu, T.-C., Heath, R. J., Becker, M. L., Fagbami, L., Horn, H., Mercer, J., Yilmaz, O. H., Jaffe, J. D., Shamji, A. F., Bhan, A. K., Carr, S. A., Daly, M. J., Virgin, H. W., Schreiber, S. L., Stappenbeck, T. S. & Xavier, R. J. Atg16L1 T300A variant decreases selective autophagy resulting in altered cytokine signaling and decreased antibacterial defense. Proc. Natl. Acad. Sci. U.S.A. 111, 7741–7746 (2014).Fagbami, L., Kuhn, E., Abbatiello, S.E., Carr, S.A. Automated Plasma Processing for Quantitative, Targeted LC/MS Analysis of Proteins. Agilent Technologies Application Note. online: (2013).AWARDSNIH F31 Ruth L. Kirschstein National Research Service Award (NRSA) Individual Predoctoral FellowshipHarvard Graduate School Leadership Institute (HGSLI) FellowBridging the Gaps Community Health Internship Program (BTG-CHIP) Fellow Summer Undergraduate Research at Emory (SURE) FellowHoward Hughes Medical Institute Exceptional Research Opportunities Program (HHMI-EXROP) ScholarNational Merit ScholarRESEARCH EXPERIENCEBroad Institute of Harvard & MITJul 2011 – Aug 2014BiochemistCambridge, MAUsed quantitative proteomics to conduct routine analysis of complex biological samples in support of the discovery and characterization of protein interactions and pathways, biomarkers and drug targets in various disease states. Developed automated, high-throughput methods to accelerate the discovery of protein interactomes and protein modification networks.Merck Research LaboratoriesJan 2010 – Jun 2011Research BiochemistWest Point, PAConducted comprehensive and comparative large scale proteomics of complex biological samples and recombinant protein products to support the discovery and characterization of novel vaccine candidates. Developed assays for optimized protein identification, analysis and quantification.Taylor Technology, Inc.Sep 2009 – Dec 2009Discovery Chemist (Contract Position)Princeton, NJConducted sample analysis for biological samples using manual and automated sample preparation systems. Developed and executed methods for the analysis of biomolecules in support of bioavailability and drug metabolism studies. Developed LC-MS/MS methods for multi-analytes, metabolites and prodrugs on a variety of instrument platforms. BTG-CHIP Fellow, PPSNJJun 2009-Aug 2009Community Health InternshipCamden, NJConducted a comprehensive assessment of strengths and needs of the male clinic. Planned information seminars on sexual health resources, nutritional information, and oral health care for infants and toddlers. Created a STI learning game to encourage community participation in reproductive health seminars. Skills utilized include key informant interviews, needs assessment, and report writing.Quest Pharmaceutical ServicesOct 2006 - Dec 2007Associate ScientistNewark, DEConducted sample analysis for biological samples using manual and automated sample preparation systems. Developed and executed methods for the LC-MS/MS analysis of biomolecules in support of pharmacokinetic, tissue distribution, and bioavailability studies.Emory UniversityJan 2004-May 2006Lynn Lab, Student Researcher (DNA Template Project)Atlanta, GADeveloped new model for the synthesis and polymerization of an amino-carbonate deoxy-nucleoside analog. Explored a reaction sequence that accurately controls polymerization along a DNA template to give sequence specific and chain length specific materials. Probed said sequence with synthetic materials in order to accurately read polymeric templates in the absence of biological catalysts.INVITED TALKS2019SAPIENZA Università di Roma Dipartimento di Sanità PubblicaAug 23Rome, ItalyArginine enables the Adaptive Proline Response to Halofuginone in P. falciparum2018 Broad Institute Annual RetreatDec 17-18 Boston, MAHow Metabolism Drives Drug Resistance in Malaria Parasites2017 ASTMH Symposium: Landscape of the Druggable Plasmodium GenomeNov 5-9Baltimore, MDProline Homeostasis: A Novel Mediator of Drug Resistance in Plasmodium falciparumCONFERENCE TALKS2017 Molecular Parasitology MeetingSep 10-14Woods’ Hole, MAInterrogating the Mechanism of Non-Genetic Drug Resistance in P. falciparum*Best Poster Award2016 UAE Graduate Student Research ConferenceApril 27-28Al Ain, UAEHalofuginone Induces A Non-Genetic Drug Resistance Phenotype in P. falciparum*Best Talk AwardTEACHING EXPERIENCEHarvard T.H. Chan School of Public Health Department of Immunology & Infectious Diseases Aug 2017-Oct 2017IMI201a - Ecology, Epidemiology & Control of Important Parasitic DiseasesProf Dyann WirthHarvard Chemical Biology Program Jan 2017 - May 2018CB2200 - Introduction to Chemical BiologyProf Ralph Mazitschek*Distinction in Teaching AwardHarvard Department of Stem Cell & Regenerative Biology Sep 2015-Dec 2015SCRB230 - Principles & Practices of Drug DevelopmentProf Lee RubinEmory Chemistry Department Sep 2005-May 2006ECFS190 - Origins of OrderProf David LynnSKILLSBenchtop: analyte extraction and quantitation; bacterial protein expression & purification; chemical and metabolic labeling; CRISPR/Cas9 genome editing; immunoprecipitation; molecular biology techniques; organic synthesis; protein extraction, separation, and quantitationBioinformatics: Analyst, Bioworks, ChemOffice, Elucidator, Empower, EndNote, GIS, GraphPad Prism, MassLynx, MaxQuant, PEAKS, SciFinder, SigmaPlot, SpectrumMill, SPSS, XCalibur, VectorNTI, VWorks, Watson LIMS Compliance: cGMP, CITI, GLP, HIPAAInstrumentation: Agilent Bravo, Confocal Microscopy, FTMS, Hamilton Robotics, HPLC (preparative, analytical & nano-flow), IMS, LCMS, LCMS2, MALDI-TOF, MRM, NMR, TomTec, UV, UPLCPolicy: data coding and analysis; focus group and key informant interviews; needs assessment; survey design; report writing Programming: C++, JavaScript, MATLAB, R, Visual Basic Transferrable: independent and motivated self-starter; excellent communication and writing skills; outstanding prioritizing and multitasking ability; highly effective in multidisciplinary collaborationsSERVICE & OUTREACHSkillset: advocacy, editing, fundraising, grant review, mentoring, public speaking, recruiting, social media, strategy, teaching, writingAcademicBiology of Parasitism (BoP) Social Media CoordinatorJournal of Emerging Investigators (JEI) Editor Science in the News LecturerBoards & CommitteesHarvard College Safety CommitteeHarvard Cooperative Society (COOP) Director HMS Graduate Education Review Subcommittee Minority Biomedical Scientists of Harvard (MBSH) Executive CommitteeMentoringBiological Sciences in Public Health (BPH) Summer Internship Mentor Deborah’s Promise Preteen MentorHarvard Graduate School Leadership Institute (HGSLI) Leadership TeamMORE (Multicultural Outreach and Resources at Emory) Mentor ................
................

In order to avoid copyright disputes, this page is only a partial summary.

Google Online Preview   Download