TROUBLESHOOTING SODIUM DODECYL SULFATE- …
[Pages:3]TROUBLESHOOTING SODIUM DODECYL SULFATEPOLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE)
Troubleshooting This troubleshooting document gives the problem, possible cause and suggested solution for problems during the SDS-PAGE application:
Problem: Weak of missing protein bands
The protein/antigen quantity Increase the sample concentration.
is below the detection level of Use a more sensitive stain.
the stain
The proteins are not fixed in Use a stain which will fix the proteins.
the gel
Use a gel fixing solution.
Proteins have ran off the gel Use a SDS-PAGE gel with a higher % acrylamide.
Proteins are degraded
Make sure there is no protease contamination.
Ensure the samples did not freeze-thaw.
The small-peptides ( ................
................
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