Chapter 1: Exploring Life



Chapter 20: DNA Technology and Genomics

• Intro- DNA sequencing, recombinant DNA, in vitro, genetic engineering, biotechnology

20.1 DNA cloning permits production of multiple copies of a specific gene or other DNA segment

• Intro – gene cloning

• DNA Cloning and Its Applications

• Using Restriction Enzymes to Make Recombinant DNA- restriction enzymes, restriction sites, restriction fragments, sticky ends, DNA ligase

• Cloning a Eukaryotic Gene in a Bacterial Plasmid- cloning vector

• Storing Cloned Genes in DNA Libraries- genomic library, cDNA library, in-vitro

• Amplifying DNA- polymerase chain reaction (PCR)

Concept Check- none

20.2 Restriction fragment analysis detects DNA differences that affect restriction sites

• Gel Electrophoresis and Southern Blotting – gel electrophoresis, restriction fragment analysis, Southern blotting

• Restriction Fragment Length Differences as Genetic Markers- RFLPs, restriction fragment length polymorphisms

Concept Check- 2

20.3 Entire genomes can be mapped at the DNA level

• Intro- Human Genome Project

• Genetic (Linkage) Mapping: Relative Ordering of Markers- linkage mapping, genetic markers

• Physical Mapping: Ordering DNA Fragments- physical mapping

• DNA Sequencing- DNA Sequencing

Concept Check- 1

20.4 Genome sequences provide clues to important biological questions

• Intro- genomics

• Determining Gene Function- in vitro mutagenesis

• Future Directions in Genomics- proteomics,

Concept Check- 1,3

20.5 The practical applications of DNA technology affect our lives in many ways

• Medical Applications- gene therapy

• Forensic Evidence- DNA fingerprints

• Agricultural Applications- transgenic organisms, Ti plasmid

• Safety and Ethical Questions Raised by DNA Technology- genetically modified (GM) organisms

Concept Check- 1,2

Testing Your Knowledge 1, 2, 3, 4, 6, 7, 8, 9, 10

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