Cryopreservation of Mouse Spermatozoa

Cryoprotectant: DMSO. Mineral oil. Surgical equipment. Method. Remove cryotubes from liquid nitrogen and place them on the bench at room temperature until thawed (usually it requires about 10-15 min). When completely thawed, slowly add 0.8 ml of PBS in a drop wise fashion to dilute the DMSO. ................
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