ADCC Assay Services Quotation Form
Instructions
|1. Please read and complete the form carefully due to the complexity of the immune cell stimulation and cytokine release assay |
|2. Please email the completed form to bioassay@ |
|3. Our service representative will contact you as soon as possible |
Customer Information
|Name: |
|Phone: |
|Institution/Company: |
|Shipping address: |
|Email: |
Immune Cell Stimulation and Cytokine Release Assay Service (SC-1589)
|Do you need a monoclonal antibody as the positive control? |
|Yes, I need. I will provide the antibody. |
|Yes, I need. But I don’t have the antibody. |
|No, I don’t need. |
|Note: |
|1. Positive control antibodies, which are produced according to the published sequences and functionally validated in-house, are available at GenScript. A |
|list is provided in the Appendix at the end of this form. |
|2. Positive control antibodies provided by GenScript will NOT be charged for the assay service, whereas client’s positive controls will be treated as |
|samples. |
|Number of Test Antibodies: |
|Note: |
|GenScript’s positive and negative controls are not included if inclusion of control antibody is applicable. |
|Nature of Test Antibodies: |
|Human; Mouse; Human & Mouse Chimeric; Fc fusion protein; Others: ; |
|IgG1; IgG2; IgG3; IgG4; Others: |
| |
|Note: |
|Selection of antibody class will help us to decide what negative control to be included in the assay, e.g. if your antibody sample belongs to IgG4 class, an|
|irrelevant human IgG4 antibody will be used as the negative control. |
|Name of Target Molecule: |
| |
|Note: |
|If the name of the target molecule cannot be disclosed at this stage, please leave it blank. |
|Name of Effector Cells: |
|CD3+ T cells; CD4+ T cells; CD8+ T cells; |
|Monocytes; NK cells; Dendritic Cells; Monocyte-Derived Macrophages; |
|PBMCs; Whole blood; Other, please specify: |
|Stimulator Options: |
|PHA; PMA + Ionomycin; LPS; Anti-CD3 antibody (OKT3); |
|Anti-CD3/Anti-CD28 beads; Superantigen (e.g. SEA, SEB, SEE, etc.), please specify: ; |
|Dendritic Cells; Raji cells; Other tumor cell lines, please specify: |
|Other stimulating factors (e.g. cytokines, small molecules, etc., please specify: |
|Co-factors: |
|Please specify other co-factors to be added in the stimulation experiment if there is any: |
|a. Co-factor 1: , immune stimulation; immune suppression; unknown; |
|b. Co-factor 2: , immune stimulation; immune suppression; unknown; |
|c. Co-factor 3: , immune stimulation; immune suppression; unknown; |
|Immune Cell Stimulating Conditions: |
|a. Effector cell seeding density: cells/well (96-well plate); |
|Optimization required; Use GenScript’s default value, obtained from past experience; |
|b. Serum concentration: %; |
|Optimization required; Use GenScript’s default value, obtained from past experience; |
|c. Duration of stimulation: hrs; |
|Optimization required; Use GenScript’s default value, obtained from past experience; |
|d. Concentration of stimulator: ng/ml or cells/well |
|Optimization required; Use GenScript’s default value, obtained from past experience; |
|e. Concentration of co-factor 1: |
|Optimization required; |
|f. Concentration of co-factor 2: |
|Optimization required; |
|g. Concentration of co-factor 3: |
|Optimization required; |
|Parameters to be assessed: |
|IL-2; IFN-γ; TNFα; GM-CSF; Other, please specify: ; |
|Multiplex Cytokine Release Assay (Luminex), |
|please specify the cytokine collection: ; |
|please specify the link to the kit if it is already available: ; |
|Multiplex Cytokine Release Assay (MSD), |
|please specify the cytokine collection: ; |
|please specify the link to the kit if it is already available: ; |
|Dose-response Curve: |
|8 doses (including 0 ug/ml) in triplicate. |
|Starting concentration by default: 10 μg/ml; dilution factor: 3; |
|Customized starting concentration: μg/ml; dilution factor: |
|Optimization is required. |
|Assessment of Antigen Expression Level of Target Cell by FACS: |
|Yes, I need. If “Yes” is selected, please provide the name of the target: |
|No, I don’t need. |
| |
|Note: |
|If “No” is selected, the customer needs to provide evidence of target expression on the target cells. |
|Please specify any other special requirements: |
| |
Project Information
|Is this project for grant application purpose? Yes No |
|When will the project start? Immediately Within one month Within three months Half a year later |
Note: Final report contains raw data, processed data, protocol summary, dose-response curve and EC50 value for each antibody sample.
Appendix
List of positive control antibodies available at GenScript, which are produced in-house according to their published sequences.
Anti-CD40 agonist antibody
Anti-OX40 agonist antibody
Anti-GITR agonist antibody
Anti-4-1BB agonist antibody
Hu-5F9-G4 (anti-CD47)
IMAB362 (anti-Claudin 18.2)
IMAB027 (anti-Claudin 6)
Tecentriq (anti-PD-L1)
Keytruda (anti-PD-1)
Opdivo (anti-PD-1)
25F7 (anti-LAG3)
OSE172 (anti-Sirpα)
NC318 (anti-Siglec-15)
Yervoy (anti-CTLA-4)
22G2 (anti-TIGIT)
Siltuximab (anti-IL-6)
F7-G4 (anti-CXCR4)
Rituxan (anti-CD20)
Herceptin (anti-Her2)
Humira (anti-TNFα)
Erbitux (anti-EGFR)
Darzalex (anti-CD38)
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Immune Cell Stimulation and Cytokine Release Assay Service Quotation Form
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