Yeast Synthetic Drop-Out Media
Yeast Synthetic Drop-Out Media
Per Liter
Yeast Nitrogen Base (w/o amino acids and ammonium sulfate) 1.6 g
Ammonium Sulfate 5.0 g
Potassium Chloride 7.6 g
Yeast Synthetic Media (Sigma product number Y2001) 1.4 g
Supplemented amino acids (as needed)
Uracil 10 ml
Leucine 6 ml
Histidine 2 ml
Tryptophan 2 ml
Bring to 900 ml and add 20 g agar (if needed)
In a separate flask:
20 g glucose
Bring to 100 ml
Autoclave for 15 minutes
|Constituent |Final mg/L |Stock Solution per 100 ml H2O |ml stock for 1L media |
|Uracil* |20 |200 mg |10 |
|Leucine |60 |1 g |6 |
|Histidine |20 |1 g |2 |
|Tryptophan |20 |1 g |2 |
* store at room temperature
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