Yeast Synthetic Drop-Out Media



Yeast Synthetic Drop-Out Media

Per Liter

Yeast Nitrogen Base (w/o amino acids and ammonium sulfate) 1.6 g

Ammonium Sulfate 5.0 g

Potassium Chloride 7.6 g

Yeast Synthetic Media (Sigma product number Y2001) 1.4 g

Supplemented amino acids (as needed)

Uracil 10 ml

Leucine 6 ml

Histidine 2 ml

Tryptophan 2 ml

Bring to 900 ml and add 20 g agar (if needed)

In a separate flask:

20 g glucose

Bring to 100 ml

Autoclave for 15 minutes

|Constituent |Final mg/L |Stock Solution per 100 ml H2O |ml stock for 1L media |

|Uracil* |20 |200 mg |10 |

|Leucine |60 |1 g |6 |

|Histidine |20 |1 g |2 |

|Tryptophan |20 |1 g |2 |

* store at room temperature

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