Achievement of Protein Thermostability by Amino Acid ...

Achievement of Protein Thermostability by

Amino Acid Substitution

2018/6/30 M1 Majima Sohei

Contents of Today's seminar

? Introduction

? Study on hyperthermophilic enzymes to understand the origin of thermostability

? Acquiring thermostability by amino acid substitution

? Summary

2

Introduction

New type of synthesis:

combination of chemocatalyst and biocatalyst

Bornscheuer et al. Nature Catalysis, 2018, 1, 12

3

Introduction

New type of synthesis:

combination of chemocatalyst and biocatalyst

Denaturation in organic solvent, high temperature

Bornscheuer et al. Nature Catalysis, 2018, 1, 12 4

Introduction

Organic reactions in aqueous media

NHK reaction

Micskei et al. Tetrahedron Lett. 2001, 42, 7711

Heck reaction

Suzuki-Miyaura coupling

*CTAB = C16H32NMe3Br, amphiphilic molecule

Bhattacharya et al. Tetrahedron Lett. 2005, 46, 3557

5

Introduction

Examples of the reactions

combination of Suzuki-Miyaura coupling and enzymatic stereoselective reduction of ketone

Cool down, pH adjustment is necessary

Burda et al, Angew. Chem. Int. Ed. 2008, 47, 9551 6

Introduction

Examples of the reactions

combination of Ru-catalyzed olefin isomerization and enzymatic stereoselective reduction of ketone.

Cool down is necessary

Sabin, et al. Angew. Chem. Int. Ed. 2016, 55, 8691

7

Introduction

New type of synthesis:

combination of chemocatalyst and biocatalyst

Thermostability is important for one-pot reaction

Bornscheuer et al. Nature Catalysis, 2018, 1, 12 8

 ................
................

In order to avoid copyright disputes, this page is only a partial summary.

Google Online Preview   Download