Osmosis and Diffusion Practice - Mr. Aragon



Osmosis and Diffusion Practice Name:

I. Based on what you’ve learned, in your own words, answer the following questions regarding movement of materials through a cell membrane. DO NOT COPY and PASTE!!!

1. What does semi-permeable mean? **

2. What is the net movement of molecules from high to low concentrations? **

3. What is the term for the diffusion of water? **

4. What does dynamic equilibrium mean? **

5. What is a hypertonic solution? **

6. What is a hypotonic solution? **

7. What is an isotonic solution? **

II. Observe the diagrams in the table below. Assume that the dots are dissolved particles on either side of the cell membrane. They are like oxygen molecules that can go across the membrane. Do the following situations represent concentration gradients? If so, in which direction would diffusion occur?

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| | | |

|gradient? |** |gradient? |** |gradient? |** |

|Yes or No | |Yes or No | |Yes or No? | |

|movement |** |movement |** |movement |** |

|left, right, or none | |left, right, or none | |left, right, or none | |

III. Observe the diagrams in the table below. Assume that the dots are dissolved particles (like

protein or carbohydrate molecules) on either side of the cell membrane. Do the following situations represent concentration gradients? If so, in which direction would osmosis occur?

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| | | |

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| | | |

| | | |

| | | |

| | | |

|gradient? |** |gradient? |** |gradient? |** |

|Yes or No | |Yes or No | |Yes or No? | |

|movement |** |movement |** |movement |** |

|left, right, or none | |left, right, or none | |left, right, or none | |

IV. Observe the table below. Are the following hypotonic, hypertonic, or isotonic solutions? Which way will water mostly move? (some situations may have water moving equally)

|intracellular fluid |extracellular fluid |Hypotonic, |water moves mostly |

|(inside the cell) |(outside of the cell) |Hypertonic, |inside or outside the cell |

| | |Isotonic | |

|5% salt |10% salt |** |** |

|10% salt |10% salt |** |** |

|3% glucose |1% glucose |** |** |

|2% protein |1% protein |** |** |

|9% salt |9% salt |** |** |

|13% water |25% water |** |** |

|59% water |45% water |** |** |

|90% water |92% water |** |** |

|74% glucose |87% glucose |** |** |

V. Observe the diagram below and answer the questions.

[pic]

1. Can you tell if the cell is in a hypertonic, hypotonic, or isotonic solution? EXPLAIN!

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2. What will happen to the cell if it is placed in a 50% salt solution?

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3. What will happen if the cell is placed in pure water?

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OSMOSIS WORKSHEET

Below are animal cells placed in beakers of various concentrations.

1. Draw an arrow to show which way the water would move by osmosis

2. Identify the type of solution (hypertonic/hypotonic/isotonic)

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40% H2O

60% Solute

90% H2O

10% Solute

85% H2O 15% Solute

90% H2O 10% Solute

The solution is: hypertonic / hypotonic / isotonic

Water will: not move / move into the cell / move out of the cell

The solution is: hypertonic / hypotonic / isotonic

Water will: not move / move into the cell / move out of the cell

45% H2O

55% Solute

75% H2O

25% Solute

80% H2O 20% Solute

75% H2O 25% Solute

The solution is: hypertonic / hypotonic / isotonic

Water will: not move / move into the cell / move out of the cell

The solution is: hypertonic / hypotonic / isotonic

Water will: not move / move into the cell / move out of the cell

50% H2O

50% Solute

90% H2O

10% Solute

63% H2O 37% Solute

50% H2O 50% Solute

The solution is: hypertonic / hypotonic / isotonic

Water will: not move / move into the cell / move out of the cell

The solution is: hypertonic / hypotonic / isotonic

Water will: not move / move into the cell / move out of the cell

82% H2O

18% Solute

90% H2O

10% Solute

80% H2O 20% Solute

25% H2O 75% Solute

The solution is: hypertonic / hypotonic / isotonic

Water will: not move / move into the cell / move out of the cell

The solution is: hypertonic / hypotonic / isotonic

Water will: not move / move into the cell / move out of the cell

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