LABORATORY SAFETY MANUAL - EHS

LABORATORY SAFETY MANUAL

DECEMBER 2022 EDITION

Environmental Health and Safety Department University of Washington Box 354400 Seattle, WA 98195-4400 Phone: 206.543.7262 FAX: 206.543.3351 ehs.washington.edu

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This policy and procedures manual was developed by the University of Washington Environmental Health and Safety (EH&S) Department to provide information to protect workers and the surrounding environment and to achieve compliance with applicable standards and regulations. The University of Washington Institutional Chemical and Physical Safety Committee (ICAPS) approved this revision with the expectation that updates will be necessary as changes in regulations, policies and procedures dictate.

Implementation of these policies and procedures is the responsibility of the Principal Investigator and depends largely on the efforts of laboratory supervisors and employees. It is essential that they seek additional advice and training when needed to conduct research in a manner that is safe to employees, students, and the surrounding community. To assist in this endeavor, the services of the Environmental Health and Safety Department are available at the University of Washington.

December 5, 2022 Page 2 of 261

TABLE OF CONTENTS

EMERGENCY INFORMATION............................................................................................................................................... 12 LABORATORY-SPECIFIC INFORMATION ............................................................................................................................ 13 SECTION 1: LABORATORY RESPONSIBILITIES................................................................................................................... 17

A. PURPOSE ................................................................................................................................................................. 18 1. Chemical Hygiene Plan (CHP) ........................................................................................................................... 18 2. Regulations Pertaining to the Chemical Hygiene Plan .................................................................................. 18 3. Chemical Hygiene Plan Accessibility ................................................................................................................ 19 4. Other Plans and References ............................................................................................................................. 19 5. Applicability to Students.................................................................................................................................... 19

B. SCOPE AND APPLICATION..................................................................................................................................... 20 1. Chemical Laboratory.......................................................................................................................................... 20 2. Chemical and Non-Chemical Hazards ............................................................................................................. 20

C. RESPONSIBILITIES................................................................................................................................................... 21 1. Responsible Party (RP) and Chemical Hygiene Officer (CHO) ...................................................................... 21 2. Dean, Department Chair and Director ............................................................................................................ 26 3. Employees/Students .......................................................................................................................................... 27 4. Environmental Health and Safety Department.............................................................................................. 28 5. UW Chemical Hygiene Officer (UW CHO) ........................................................................................................ 28 6. Institutional Chemical and Physical Safety Committee (ICAPS) ................................................................... 28

SECTION 2 - CHEMICAL MANAGEMENT ............................................................................................................................ 31 A. BASIC LABORATORY SAFETY PRACTICES ............................................................................................................. 33 1. Working Alone .................................................................................................................................................... 33 2. Prevent Chemical Exposure .............................................................................................................................. 33 3. Washing Hands................................................................................................................................................... 34 4. Food and Drink ................................................................................................................................................... 34 5. Access to Emergency Exits and Equipment .................................................................................................... 34 6. Laboratory Signs ................................................................................................................................................ 34 7. Housekeeping ..................................................................................................................................................... 35 8. Sharps Safety ...................................................................................................................................................... 35 B. CHEMICAL INVENTORY AND THE SAFETY DATA SHEET (SDS)........................................................................... 36 1. Access to MyChem ............................................................................................................................................. 36

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Page 3 of 261 December 5, 2022 2. Conducting Your Chemical Inventory.............................................................................................................. 36 3. Safety Data Sheet (SDS) ..................................................................................................................................... 37 C. CHEMICAL PROCUREMENT ................................................................................................................................... 37 1. Hazardous Chemicals ........................................................................................................................................ 37 2. Pharmaceuticals ................................................................................................................................................. 38 3. DEA Controlled Substances .............................................................................................................................. 38 4. Non-Denatured Ethyl Alcohol ........................................................................................................................... 38 5. Radioactive Materials......................................................................................................................................... 38 6. Highly Dangerous Materials ............................................................................................................................. 38 7. Compressed Gas Cylinder Procurement ........................................................................................................ 39 8. Chemical Exchange ............................................................................................................................................ 39 9. Additional exceptions ........................................................................................................................................ 39 D. CHEMICAL STORAGE .............................................................................................................................................. 39 1. Evaluate Chemical Hazards for Storage .......................................................................................................... 39 2. Chemical Storage Practices............................................................................................................................... 40 3. Chemical Storage Quantity Limits.................................................................................................................... 43 E. CHEMICAL LABELING ............................................................................................................................................. 44 1. Original Container .............................................................................................................................................. 44 2. Labeling Stock / Working Solutions ................................................................................................................. 45 F. TRANSPORTING CHEMICALS................................................................................................................................. 46 1. Transporting between Floors and Buildings on Campus ............................................................................. 47 2. Transporting Chemicals off Campus ............................................................................................................... 48 3. Receiving Chemical Shipments......................................................................................................................... 48 G. SPECIAL CHEMICAL HAZARDS............................................................................................................................... 49 1. Highly Dangerous Chemicals............................................................................................................................ 49 2. Particularly Hazardous Substances ................................................................................................................. 49 3. Reactive Chemicals ............................................................................................................................................ 50 4. Potentially Explosive Chemicals ....................................................................................................................... 52 5. Highly Toxic Substances .................................................................................................................................... 55 6. Carcinogens and Reproductive Hazards ......................................................................................................... 56 7. Sensitizing or Allergenic Chemicals ................................................................................................................. 57 8. Synthesized Chemicals ...................................................................................................................................... 58 9. Compressed Gases, Gas Cylinders and Liquid Cryogen Containers ........................................................... 59 10. Flammable and Combustible Liquids.......................................................................................................... 62

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December 5, 2022 Page 4 of 261 11. Homeland Security Chemicals of Interest .................................................................................................. 64 12. Process Safety for Highly Hazardous Chemicals ....................................................................................... 66 13. Chemotherapy and Hazardous Drugs ........................................................................................................ 66

H. REGULATED HAZARDOUS CHEMICALS LISTED UNDER THE WAC WASHINGTON VERTICAL STANDARD ... 66 SECTION 3: CHEMICAL WASTE MANAGEMENT ................................................................................................................ 69

A. HAZARDOUS CHEMICAL WASTE RESPONSIBILITIES .......................................................................................... 71 1. Laboratory Workers ........................................................................................................................................... 71 2. UW EH&S Environmental Programs ................................................................................................................ 71

B. WHAT QUALIFIES AS HAZARDOUS WASTE? ........................................................................................................ 71 1. Flammable/Ignitable .......................................................................................................................................... 71 2. Corrosive ............................................................................................................................................................. 72 3. Reactive ............................................................................................................................................................... 72 4. Toxic ..................................................................................................................................................................... 72 5. Carcinogenic ....................................................................................................................................................... 74 6. Waste Evaluation Request................................................................................................................................. 75

C. HAZARDOUS WASTE ACCUMULATION RULES .................................................................................................... 75 1. Appropriate Containers..................................................................................................................................... 75 2. Hazardous Waste Labels ................................................................................................................................... 75 3. Segregation ......................................................................................................................................................... 76 4. Accumulation Volume Limits ............................................................................................................................ 76 5. Large Containers (Drums) ................................................................................................................................. 77 6. Inherently Waste-like Chemicals ...................................................................................................................... 77

D. HAZARDOUS WASTE COLLECTION REQUESTS ................................................................................................... 77 1. Hazardous Waste Collection Overview ........................................................................................................... 77 2. Collection Requests ? One-Time ...................................................................................................................... 77 3. Routine Collection Requests ............................................................................................................................. 77 4. Waste Cleanouts................................................................................................................................................. 78 5. What Happens to Hazardous Waste? .............................................................................................................. 78

E. TRASH DISPOSAL .................................................................................................................................................... 78 1. Trash Disposal of Chemicals............................................................................................................................. 79 2. Trash Disposal of Empty Chemical Containers .............................................................................................. 79 3. Trash Disposal of Contaminated Items........................................................................................................... 80

F. DISCHARGES TO THE SANITARY SEWER .............................................................................................................. 80 1. King County Local Sewer Discharge Limits ..................................................................................................... 80

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Page 5 of 261 December 5, 2022 2. Outside King County .......................................................................................................................................... 81 3. Treating Hazardous Wastes.............................................................................................................................. 81 4. Soaps, Bleach and Acetone............................................................................................................................... 81 5. Scintillation Fluids .............................................................................................................................................. 81 6. Dilution Prohibition............................................................................................................................................ 81 G. CHEMICAL WASTES OF PARTICULAR CONCERN................................................................................................. 82 1. Unknown Chemicals .......................................................................................................................................... 82 2. Potentially Explosive Wastes ............................................................................................................................ 82 3. Peroxide-Forming Chemicals............................................................................................................................ 82 4. Picric Acid and Other Polynitroaromatic Compounds .................................................................................. 82 5. Legacy Chemicals ............................................................................................................................................... 83 H. HAZARDOUS WASTE MINIMIZATION ................................................................................................................... 83 1. Treatment and Recycling in the Laboratory ................................................................................................... 83 2. Hazardous Materials Recycling ........................................................................................................................ 83 I. SOLID WASTE AND RECYCLING ............................................................................................................................... 83 1. Paper and Cardboard ........................................................................................................................................ 83 2. Plastic and Glass ................................................................................................................................................. 84 3. Packaging Materials ........................................................................................................................................... 84 4. Printer Cartridges ............................................................................................................................................... 84 5. Batteries .............................................................................................................................................................. 84 6. Metallic Lead ....................................................................................................................................................... 84 J. SHARPS, LAB GLASS AND PLASTIC .......................................................................................................................... 84 1. Sharps .................................................................................................................................................................. 84 2. Trace Chemo Sharps.......................................................................................................................................... 85 3. Biohazardous Glass and Plastic Items............................................................................................................. 86 4. Lab Glass ............................................................................................................................................................. 86 K. INFECTIOUS OR BIOLOGICAL WASTE................................................................................................................... 86 L. RADIOACTIVE WASTE ............................................................................................................................................. 87 M. MIXED WASTE.......................................................................................................................................................... 87 N. LIQUID SCINTILLATION COCKTAILS ..................................................................................................................... 87 O. ANIMALS AND ANIMAL BY-PRODUCTS................................................................................................................ 87 1. Contaminated Animals and Animal By-Products........................................................................................... 87 2. Non-Contaminated Animals and Animal By-Products .................................................................................. 87 P. GAS CYLINDERS ...................................................................................................................................................... 87

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December 5, 2022 Page 6 of 261 SECTION 4 - LABORATORY EQUIPMENT AND FACILITIES ............................................................................................... 89 A. EMERGENCY EYEWASHES AND SHOWERS .......................................................................................................... 91 1. Eyewash Stations................................................................................................................................................ 91 2. Safety Showers ................................................................................................................................................... 91 3. Deluge Hoses ...................................................................................................................................................... 91 B. FIRE SAFETY EQUIPMENT ...................................................................................................................................... 91 1. Fire Extinguishers ............................................................................................................................................... 91 2. Flammable Liquid Storage Cabinets ................................................................................................................ 93 3. Flammable Storage Refrigerators .................................................................................................................... 93 C. LABORATORY SIGNS .............................................................................................................................................. 94 1. Lab Caution Sign................................................................................................................................................. 94 2. Emergency Procedures for Laboratories ........................................................................................................ 95 3. Laboratory Floor Plan ........................................................................................................................................ 96 4. Emergency/Safety Equipment Location Signs................................................................................................ 96 5. Hazards Warning Signs...................................................................................................................................... 96 6. Natural Gas Emergency Shut Off Valve ........................................................................................................... 96 7. Laboratory Water ? Do Not Drink .................................................................................................................... 96 D. LABORATORY VENTILATION.................................................................................................................................. 96 1. Laboratory Design.............................................................................................................................................. 96 2. Fume Hoods........................................................................................................................................................ 96 7. Perchloric Fume Hoods ................................................................................................................................... 101 8. Glove Boxes ...................................................................................................................................................... 101 9. Biological Safety Cabinets ............................................................................................................................... 101 10. Laminar Flow Hoods....................................................................................................................................101 11. Ductless Laboratory Hoods ........................................................................................................................ 102 12. Cold Rooms, Warm Rooms and Environmental Chambers ...................................................................102 13. Other Ventilation Systems .......................................................................................................................... 103 14. Maintenance of Ventilation Systems ......................................................................................................... 103 E. OTHER FACILITY CONDITIONS ............................................................................................................................ 105 1. General Laboratory Environment .................................................................................................................. 105 2. Electrical Hazards ............................................................................................................................................. 107 3. Lockout / Tagout Concerns ............................................................................................................................. 109 4. Equipment Guards and Mounting ................................................................................................................. 109 5. Confined Spaces...............................................................................................................................................109

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Page 7 of 261 December 5, 2022 F. LABORATORY APPARATUS AND EQUIPMENT...................................................................................................109

1. General Safety Work Practices ....................................................................................................................... 110 2. Refrigerators and Freezers ............................................................................................................................. 110 3. Stirring and Mixing Equipment.......................................................................................................................111 4. Heating Equipment .......................................................................................................................................... 111 5. Ultrasonicators ................................................................................................................................................. 115 6. Centrifuges ........................................................................................................................................................ 116 7. Rotary Evaporators .......................................................................................................................................... 116 8. Autoclaves ......................................................................................................................................................... 116 9. Vacuum Systems .............................................................................................................................................. 116 10. Equipment maintenance ............................................................................................................................ 117 G. DECONTAMINATION OF WORK AREAS..............................................................................................................117 1. Custodial Services ............................................................................................................................................ 117 2. Servicing of Lab Area or Equipment .............................................................................................................. 117 H. DECONTAMINATION OF EQUIPMENT FOR DISPOSAL.....................................................................................118 1. Equipment Used to Process/Store Chemicals .............................................................................................. 119 2. Equipment Used to Process/Store Radionuclides ....................................................................................... 119 3. Equipment Used to Process/Store Biological Material................................................................................120 SECTION 5 - EMPLOYEE HEALTH AND PPE ..................................................................................................................... 121 A. ENVIRONMENTAL MONITORING AND MEDICAL SURVEILLANCE .................................................................. 122 1. Exposure Limits ................................................................................................................................................ 122 2. Special Chemical Air Monitoring .................................................................................................................... 123 3. Possible Over-Exposure .................................................................................................................................. 123 4. Medical Evaluations ......................................................................................................................................... 123 B. PERSONAL PROTECTIVE EQUIPMENT (PPE) ...................................................................................................... 124 1. Eye Protection...................................................................................................................................................125 2. Personal Apparel .............................................................................................................................................. 126 3. Gloves ................................................................................................................................................................ 127 4. Respirators ........................................................................................................................................................ 128 5. Hearing Protectors ........................................................................................................................................... 128 6. PPE: Outside the Laboratory .......................................................................................................................... 129 SECTION 6 - STANDARD OPERATING PROCEDURES ..................................................................................................... 131 A. STANDARD OPERATING PROCEDURES (SOPS) ................................................................................................. 132 B. SOP COMPONENTS .............................................................................................................................................. 132

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