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Appendix A: Lab Preps

Lab 1: Data Analysis and Presentation LAB PREP

(This is the prep for ONE SECTION)

Part 1: Normal Distribution

Materials:

Meter sticks

Sphygmomanometer

Stethoscope

Alcohol swabs

Metric rulers

Cloth tapes

Electronic bathroom scale

Quantity:

1/table

1/table

1/table

4/table

4/table

1/table

1-2/class

Notes:

Part 2: Correlations

Materials: Quantity: Notes:

Biol 1: General Biology CC-BY College of the Redwoods, Wendy Riggs

Lab 2: The Chemistry of Life LAB PREP

Part 1: pH Chemistry

Students will do this part in table teams (groups of 4).

Materials:

pH papers (1-14)

White plastic tray with wells (Paint trays)

Wax pencil

Forceps

SOLUTIONS TO TEST:

Distilled water

Bleach

Aspirin (with small mortar and pestle so it

can be crushed and added to water)

Lemon juice

Milk

Part 2: Buffers

Students will do this part in table teams (groups of 4).

Quantity:

1 set/table

1/table

1/table

1/table

Very small amounts

Notes:

Also used for Part 2, 3

Also used for Part 2

In beakers on the side counter

In squeeze bottle

Materials:

Baking soda (NaHCO3)

Beaker (250 mL)

0.001 M HCl (hydrochloric acid)

Balances

Scoops

Weigh papers

Part 3: Buffers in the Blood

Students will do this part in pairs.

Materials:

Ehrlenmeyer flask (50 mL)

Drinking straw

Phenol red

Quantity:

1 gram/table

2/table

4

2

Quantity:

4/table

2/table

1/table

Notes:

(two large boxes total?)

1 L per side of the room

Side counter

For baking soda

With balances

Notes:

Put these at the front table so they are clean.

Part 4: Polar and Nonpolar Compounds

Students will do this part in table teams (groups of 4).

Materials:

Test tubes

Test tube rack

Detergent

Beet juice

Chili oil

Oil

Plastic stir sticks

Biol 1: General Biology

Quantity:

2/table

1/table

on side counter on side counter on side counter on side counter

4/table

Notes:

Just a few drops/group are needed.

Just a few drops/group are needed. Just a few drops/group are needed.

About half a test tube per group

CC-BY College of the Redwoods, Wendy Riggs

Lab 3: The Microscope and Cells LAB PREP

(This is the prep for ONE SECTION of 24 students)

Part 4: The Letter 'e'

Students will do this part individually.

Materials:

Letter 'e' slides

Part 5: Colored Threads

Students will do this part individually.

Materials:

Colored thread slides

Part 6: Plant cells

Students will do this part individually.

Materials:

Quantity:

1 box

Quantity:

1 box

Quantity: Notes:

Notes:

Set out on side counter

Notes:

Set out on side counter

Microscope slides

Coverslips

Water dropper bottles

Medicine droppers

Elodea

Forceps

Small clear rulers

Part 7: Animal cells

Students will do this part individually.

Materials:

Toothpicks (flat)

Methylene blue

Toothpick disposal can

Slidewash bowls

Extra: Pond water (hay infusion)

Students will do this part individually.

Materials:

Pond water/hay infusion

1/student

1/student

1/table

1/table

2 cups

2

4/table

Quantity:

1/student

1/table

2/room

2/room

Quantity:

1 container

Students can wash and reuse their slides. Also

used in Part 7

Also used in Part 7

On side counter

To place leaf on slide

Notes:

Notes:

On side counter

Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs

Lab 4: Diffusion and Osmosis LAB PREP

(This is the prep for ONE SECTION of 24 students)

Part 1: Brownian Motion

Students will do this part individually.

Materials:

Microscope slides

Coverslips

Water dropper bottles

Carmine red powder

Quantity:

1/student

1/student

1/table

2 small watch

glasses

Notes:

Also used in Part 3 Also used in Part 3

On side counter

Part 2: Dialysis Bag Diffusion (across a semipermeable membrane)

Students will do this part in table teams.

Materials:

Beakers (400 mL)

Wax pencil

Dental floss or thread

Dialysis tubing

Phenolphthalein

1 M NaOH (Sodium hydroxide)

Iodine (I2KI)

1% Starch solution

10 ml Pipette/pipettor setup for starch

Pipette/pipettor setup

Large waste bucket for starch

Quantity:

2/table

1/table

1/table

2/table

1/table

1/table

1/table

( 10mL/table

2/class

1-2/table

1-2/class

Notes:

Dropper bottle- does not go down drain!

Dropper bottle Dropper bottle

On side counter

On side counter- specifically for starch

Part 3: Osmosis and the Cell Membrane

Students will do this part individually.

Materials:

Elodea

Forceps

5% NaCl solution

Slidewash bowls

Part 4: Experimental Design

Students will do this part in pairs

Materials:

2% agar plates

Clear plastic rulers

Potassium permanganate

Methylene blue

Cork borers

Biol 1: General Biology Spring 2015

Quantity:

2 bowls

2 pairs with

bowls

1/table

1-2/room

Quantity:

2/table

2/table

1/table

1/table

2-3 sets

Notes:

On side counter

Dropper bottle

Notes:

Use deep petri dishes for diffusion experiment

Dropper bottle

Dropper bottle

On side table

CC-BY College of the Redwoods, Wendy Riggs

Lab 5: Energy and Metabolism LAB PREPS

(This is the prep for ONE SECTION of 24 students)

Part 3: Do Energy Drinks Provide You With Energy?

Materials:

Ingredients and nutrition facts

handout

Biochemical information handout

Quantity:

1 class set (24)

Students

won't keep the

handouts.

2-3/table

should be fine.

1 class set (24)

Students

won't keep the

handouts.

2-3/table

should be fine.

Notes:

Riggs has a copy of this...and Marlena should

have a copy too. It is copywrited content, so

it cannot be included with this lab manual. It

came from a case study "Another Can of Bull?

Do Energy Drinks Really Provide a Source of

Energy?" by Cheryl D. Davis and Nancy A.

Rice (Department of Biology, Western Kentucky

University). Case was accessed by Riggs from



detail.asp?case_id=506&id=506 on 8/20/12.

Riggs has a copy of this...and Marlena should

have a copy too. It is copywrited content, so

it cannot be included with this lab manual. It

came from a case study "Another Can of Bull?

Do Energy Drinks Really Provide a Source of

Energy?" by Cheryl D. Davis and Nancy A.

Rice (Department of Biology, Western Kentucky

University). Case was accessed by Riggs from



detail.asp?case_id=506&id=506 on 8/20/12.

Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs

Lab 6: Cell Respiration LAB PREP

(This is the prep for ONE SECTION of 24 students)

PAGE 1 of 2!!!

Part 1: Starch Synthesis and Storage

Students will do this part in pairs.

Materials: Quantity: Notes:

Microscope slides 1/student

Coverslips 1/student

Iodine (I2KI) dropper bottles 1/table

Potato slices

Part 2: Starch Digesetion

Students will do this part in table teams.

Materials:

Ehrlenmeyer flask (250 mL)

Glass stirring rod

10 mL graduated cylinder

Hot plate

Hot pads

Corn starch

Weigh paper and balance

Glucose test strips

Wax pencil

Amylase

250 mL beaker

1 small piece is On side counter with cutting board and knife/

fine razor blade

Quantity: Notes:

2/table

2/table

1/table

1/table

2/table

20g/table On side counter

4 On side counter

2/table On side counter

1/table Also used in Part 3

2mL/table On side or front counter

(So students can measure the water)

NOTE! This is PAGE 1 of 2!!!

There is MORE on the BACK!

Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs

Lab 6: Cell Respiration LAB PREP

(This is the prep for ONE SECTION of 24 students)

PAGE 2 of 2!!!

Part 3: Aerobic Respiration

Students will do this part in table teams.

Materials:

Respiration apparatus (funnel attached to

stopper with glass tubing)

Ehrlenmeyer flasks (to fit respiration

apparatus) - 500 mL

Rubber stoppers (No. 7)

Large test tubes

Test tube racks

Phenol red dropper bottles (2%)

Cooked germinated pinto beans

Uncooked germinated pinto beans

Handful of dry pinto beans

Waste bucket for beans

Slotted spoons

Part 4: Anaerobic Respiration

Students will do this part in table teams.

Quantity:

1/table

3/table

2/table

6/table

1/table

1/table

1 large culture dish

1 large culture dish

For front table

2

2

Notes:

(Plus one for front teacher station)

(Plus one for front teacher station)

(Plus one for front teacher station)

Also used in Part 4 (Plus one for

front teacher station)

Also used in Part 4 (Plus one for

front teacher station)

To prep beans: soak for 1-2 nights,

covered in wet paper towels to

allow germination. On day of lab,

boil half the beans for 3-4 minutes

then cool down to room temp.

On side counter

One in each dish of beans

Materials:

Microspatulas for stirring in tubes

Water bath (37˚ C) with thermometer

Water bath (55˚ C) with thermometer

Ice bath

Yeast (in fridge)

Sugar: Lactose (with labeled scoop)

Sugar: Glucose (with labeled scoop)

Sugar: Sucrose (with labeled scoop)

Sugar: Dextrin (with labeled scoop)

Tubs for used test tubes

Balloons (9 inch)

Cloth measuring tapes

Rubber bands for test tubes (to keep

balloons on)

Biol 1: General Biology Spring 2015

Quantity:

1/table

1

1

1

2

2 culture dishes 2 culture dishes 2 culture dishes 2 culture dishes

1

5/table

1/table

5/table

Notes:

Side counter

Side counter

Side counter Side counter Side counter Side counter Side counter Side counter Side counter

CC-BY College of the Redwoods, Wendy Riggs

Lab 7: Mitosis and the Cell Cycle LAB PREPS

(This is the prep for ONE SECTION of 24 students)

Part 2: Mitosis Bead Simulations

Students will do this part in table teams.

Materials:

Chromosome bead sets

Part 3: Microscopic Mitosis

Students will do this part in table teams.

Materials:

Onion root tip mitosis slides

Whitefish mitosis slides

Part 4: Mitosis Bingo

Students will do this part in table teams.

Materials:

Slideshow of mitosis images

EXTRA SUPPLIES: Models

Students will do this part in table teams.

Materials:

Mitosis models

DNA models

Quantity:

Quantity:

1 box

1 box

Quantity:

1

Quantity:

2

Notes:

Notes:

14/2C

ZL6/113

Notes:

Email Riggs for a powerpoint file of mitosis

images, if you don't have one. But ANY images

of cells going through mitosis can be used.

Notes:

Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs

Lab 8: Meiosis and Gametogenesis LAB PREPS

(This is the prep for ONE SECTION of 24 students)

Part 1: Meiosis Bead Simulations

Students will do this part in table teams.

Materials:

Chromosome bead sets

Part 2: Independent Assortment

Students will do this part in table teams.

Materials:

Chromosome bead sets

Quantity:

Quantity:

Notes:

Notes:

Part 3: Mammalian Gametogenesis

Students will do this part in table teams.

Materials: Quantity: Notes:

Slides of ovaries 1 box HN 1/21

Slides of testes 1 box HM 1/2

EXTRA SUPPLIES: Models

Students will do this part in table teams.

Materials:

Mitosis models

DNA models

Quantity:

2

Notes:

Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs

Lab 9: Mendelian Genetics LAB PREPS

(This is the prep for ONE SECTION of 24 students)

Part 3: Probability

Students will do this part in table teams.

Materials: Quantity: Notes:

Pennies 2/pair

Part 4: Law of Independent Assortment

Students will do this part in table teams.

Materials: Quantity: Notes:

Genetic corn 9:3:3:1 box 1 box

Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs

Lab 10: Human Inheritance LAB PREPS

(This is the prep for ONE SECTION of 24 students)

Part 1: Human Traits Governed by Mendelian Genetics

Students will do this part in table teams.

Materials: Quantity: Notes:

PTC papers (just the blue packs) 1 pack/table

Part 2: Human Traits NOT Governed by Mendelian Genetics (BLOOD TYPING)

Students will do this part in pairs.

Materials: Quantity: Notes:

Blood type kits 1 box Place one bottle of synthetic blood serum and one

set of synthetic antisera on each bench.

Flat toothpicks 1 jar/table For stirring the blood and antibodies

Plastic wells 2/table

EXTRA SUPPLIES: Include the blood type key at the front table

Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs

Lab 11: DNA Structure and Function LAB PREPS

(This is the prep for ONE SECTION of 24 students)

Part 4: Wheat Germ Extraction

Students will do this part in pairs.

Materials:

Beaker (250 mL)

Graduated cylinder (10 mL)

Graduated cylinder (100 mL)

Serological pipettes (5 mL)

Pipettors (green)

Thermometers

Dawn dish detergent

Adolf's Meat Tenderizer (original

unseasoned)

Scoops for meat tenderizer

Raw untoasted wheat germ

Serological pipettes (5 mL)

Pipettors (green)

Digital balance

Weigh papers

1 M Sodium Bicarbonate

Waste buckets for wheat germ

Signes across sinks "No used

wheat germ disposal or you will be

destroyed!"

Glass DNA hooks

DNA model

Ice bath (large enough for 10

beakers)

Hot bath- 55˚C

Alcohol, 95% in freezer

Glass stirring rods

Wax pencils

Quantity:

1 /table

1/table

1/table

1/table

1/table

1/table

1/table

2 containers

2

2 containers

2

2

2

2 bottles?

2

6

1

20 mL/group

1/table

1/table

Notes:

Small personal container

On side counter

On side counter

On side counter

For sodium bicarbonate solution For sodium bicarbonate solution

Side counter Side counter

Each pair will need 5 mL

At instructor's station

At instructor's station

On side counter

On side counter- with thermometer

On side counter in ice bath

Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs

Lab 12: Diversity of Life LAB PREPS

(This is the prep for ONE SECTION of 24 students)

FIELD TRIP

NO LAB PREP

Materials: Quantity: Notes:

Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs

Lab 13: Evolution and Natural Selection LAB PREPS

(This is the prep for ONE SECTION of 24 students)

Part 1: Fork'n'beans

Students will do this part in table teams.

Materials:

Black beans

Pinto beans

Kidney beans (red beans

Navy beans (white beans)

Plastic forks

Plastic spoons

Plastic knives

Forceps

Cups or tubs to collect beans

Stopwatches

If outside:

Food trays

Meter sticks

Stakes

Yarn

If inside:

Trays with vermiculite

Coffee can strainers (with screen in

the bottom)

Quantity:

1 jar

1 jar

1 jar

1 jar

10

10

10

10

25

6

6

6

4/group

1 ball/group

2

1/table

1/table

Notes:

On side

On side

On side

On side

On side counter On side counter On side counter On side counter

On side counter

Most students have cell phones they can use...

Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs

Lab 14: Viruses, Bacteria and Epidemiology LAB PREPS

(This is the prep for ONE SECTION of 24 students)

Part 2: Bacteria and Archaea

Students will do this part on their own.

Materials:

Water fern

Razer blade

Cutting board

Glass slides

Coverslips

Part 3: Epidemiology

Students will do this part on their own.

Materials:

1 test tube rack

1 mL test tubes

Disposable 1mL pipettes

Phenol red dropper bottles

0.1 M NaOH

0.001 M HCl

Quantity:

not much

needed

Quantity:

24 (1/student)

6

Notes:

In Jeff's yard?

Notes:

In front

In front

1/table

Instructions:

1. Fill one test tube half full of 0.1M NaOH and put a small sharpie mark on the bottom of this test tube.

2. Fill 23 test tubes half full of 0.001 M HCl and leave them unmarked.

3. Put test tube rack filled with test tubes at the front of room, along with disposable pipettes.

Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs

Lab 15: Classification LAB PREPS

(This is the prep for ONE SECTION of 24 students)

Part 1: Dichotomous Key

Students will do this part in their table teams.

Materials:

6 critters specimens to classify

6-9 magnetic whiteboards

6-9 packs of magnetic

Caminalcules

Quantity:

1 set of 6/table

Notes:

Critters can vary---and all tables can have a

different assortment of critters.

Set out on the tables

Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs

Lab 16: Natural History Museum LAB PREPS

(This is the prep for ONE SECTION of 24 students)

FIELD TRIP to the NATURAL HISTORY MUSEUM

NO LAB PREP

Materials: Quantity: Notes:

Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs

Lab 17: Double Helix Movie Night LAB PREPS

(This is the prep for ONE SECTION of 24 students)

Materials: Quantity: Notes:

"Double Helix" DVD This movie is available in the CR library.

Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs

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