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Appendix A: Lab Preps
Lab 1: Data Analysis and Presentation LAB PREP
(This is the prep for ONE SECTION)
Part 1: Normal Distribution
Materials:
Meter sticks
Sphygmomanometer
Stethoscope
Alcohol swabs
Metric rulers
Cloth tapes
Electronic bathroom scale
Quantity:
1/table
1/table
1/table
4/table
4/table
1/table
1-2/class
Notes:
Part 2: Correlations
Materials: Quantity: Notes:
Biol 1: General Biology CC-BY College of the Redwoods, Wendy Riggs
Lab 2: The Chemistry of Life LAB PREP
Part 1: pH Chemistry
Students will do this part in table teams (groups of 4).
Materials:
pH papers (1-14)
White plastic tray with wells (Paint trays)
Wax pencil
Forceps
SOLUTIONS TO TEST:
Distilled water
Bleach
Aspirin (with small mortar and pestle so it
can be crushed and added to water)
Lemon juice
Milk
Part 2: Buffers
Students will do this part in table teams (groups of 4).
Quantity:
1 set/table
1/table
1/table
1/table
Very small amounts
Notes:
Also used for Part 2, 3
Also used for Part 2
In beakers on the side counter
In squeeze bottle
Materials:
Baking soda (NaHCO3)
Beaker (250 mL)
0.001 M HCl (hydrochloric acid)
Balances
Scoops
Weigh papers
Part 3: Buffers in the Blood
Students will do this part in pairs.
Materials:
Ehrlenmeyer flask (50 mL)
Drinking straw
Phenol red
Quantity:
1 gram/table
2/table
4
2
Quantity:
4/table
2/table
1/table
Notes:
(two large boxes total?)
1 L per side of the room
Side counter
For baking soda
With balances
Notes:
Put these at the front table so they are clean.
Part 4: Polar and Nonpolar Compounds
Students will do this part in table teams (groups of 4).
Materials:
Test tubes
Test tube rack
Detergent
Beet juice
Chili oil
Oil
Plastic stir sticks
Biol 1: General Biology
Quantity:
2/table
1/table
on side counter on side counter on side counter on side counter
4/table
Notes:
Just a few drops/group are needed.
Just a few drops/group are needed. Just a few drops/group are needed.
About half a test tube per group
CC-BY College of the Redwoods, Wendy Riggs
Lab 3: The Microscope and Cells LAB PREP
(This is the prep for ONE SECTION of 24 students)
Part 4: The Letter 'e'
Students will do this part individually.
Materials:
Letter 'e' slides
Part 5: Colored Threads
Students will do this part individually.
Materials:
Colored thread slides
Part 6: Plant cells
Students will do this part individually.
Materials:
Quantity:
1 box
Quantity:
1 box
Quantity: Notes:
Notes:
Set out on side counter
Notes:
Set out on side counter
Microscope slides
Coverslips
Water dropper bottles
Medicine droppers
Elodea
Forceps
Small clear rulers
Part 7: Animal cells
Students will do this part individually.
Materials:
Toothpicks (flat)
Methylene blue
Toothpick disposal can
Slidewash bowls
Extra: Pond water (hay infusion)
Students will do this part individually.
Materials:
Pond water/hay infusion
1/student
1/student
1/table
1/table
2 cups
2
4/table
Quantity:
1/student
1/table
2/room
2/room
Quantity:
1 container
Students can wash and reuse their slides. Also
used in Part 7
Also used in Part 7
On side counter
To place leaf on slide
Notes:
Notes:
On side counter
Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs
Lab 4: Diffusion and Osmosis LAB PREP
(This is the prep for ONE SECTION of 24 students)
Part 1: Brownian Motion
Students will do this part individually.
Materials:
Microscope slides
Coverslips
Water dropper bottles
Carmine red powder
Quantity:
1/student
1/student
1/table
2 small watch
glasses
Notes:
Also used in Part 3 Also used in Part 3
On side counter
Part 2: Dialysis Bag Diffusion (across a semipermeable membrane)
Students will do this part in table teams.
Materials:
Beakers (400 mL)
Wax pencil
Dental floss or thread
Dialysis tubing
Phenolphthalein
1 M NaOH (Sodium hydroxide)
Iodine (I2KI)
1% Starch solution
10 ml Pipette/pipettor setup for starch
Pipette/pipettor setup
Large waste bucket for starch
Quantity:
2/table
1/table
1/table
2/table
1/table
1/table
1/table
( 10mL/table
2/class
1-2/table
1-2/class
Notes:
Dropper bottle- does not go down drain!
Dropper bottle Dropper bottle
On side counter
On side counter- specifically for starch
Part 3: Osmosis and the Cell Membrane
Students will do this part individually.
Materials:
Elodea
Forceps
5% NaCl solution
Slidewash bowls
Part 4: Experimental Design
Students will do this part in pairs
Materials:
2% agar plates
Clear plastic rulers
Potassium permanganate
Methylene blue
Cork borers
Biol 1: General Biology Spring 2015
Quantity:
2 bowls
2 pairs with
bowls
1/table
1-2/room
Quantity:
2/table
2/table
1/table
1/table
2-3 sets
Notes:
On side counter
Dropper bottle
Notes:
Use deep petri dishes for diffusion experiment
Dropper bottle
Dropper bottle
On side table
CC-BY College of the Redwoods, Wendy Riggs
Lab 5: Energy and Metabolism LAB PREPS
(This is the prep for ONE SECTION of 24 students)
Part 3: Do Energy Drinks Provide You With Energy?
Materials:
Ingredients and nutrition facts
handout
Biochemical information handout
Quantity:
1 class set (24)
Students
won't keep the
handouts.
2-3/table
should be fine.
1 class set (24)
Students
won't keep the
handouts.
2-3/table
should be fine.
Notes:
Riggs has a copy of this...and Marlena should
have a copy too. It is copywrited content, so
it cannot be included with this lab manual. It
came from a case study "Another Can of Bull?
Do Energy Drinks Really Provide a Source of
Energy?" by Cheryl D. Davis and Nancy A.
Rice (Department of Biology, Western Kentucky
University). Case was accessed by Riggs from
detail.asp?case_id=506&id=506 on 8/20/12.
Riggs has a copy of this...and Marlena should
have a copy too. It is copywrited content, so
it cannot be included with this lab manual. It
came from a case study "Another Can of Bull?
Do Energy Drinks Really Provide a Source of
Energy?" by Cheryl D. Davis and Nancy A.
Rice (Department of Biology, Western Kentucky
University). Case was accessed by Riggs from
detail.asp?case_id=506&id=506 on 8/20/12.
Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs
Lab 6: Cell Respiration LAB PREP
(This is the prep for ONE SECTION of 24 students)
PAGE 1 of 2!!!
Part 1: Starch Synthesis and Storage
Students will do this part in pairs.
Materials: Quantity: Notes:
Microscope slides 1/student
Coverslips 1/student
Iodine (I2KI) dropper bottles 1/table
Potato slices
Part 2: Starch Digesetion
Students will do this part in table teams.
Materials:
Ehrlenmeyer flask (250 mL)
Glass stirring rod
10 mL graduated cylinder
Hot plate
Hot pads
Corn starch
Weigh paper and balance
Glucose test strips
Wax pencil
Amylase
250 mL beaker
1 small piece is On side counter with cutting board and knife/
fine razor blade
Quantity: Notes:
2/table
2/table
1/table
1/table
2/table
20g/table On side counter
4 On side counter
2/table On side counter
1/table Also used in Part 3
2mL/table On side or front counter
(So students can measure the water)
NOTE! This is PAGE 1 of 2!!!
There is MORE on the BACK!
Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs
Lab 6: Cell Respiration LAB PREP
(This is the prep for ONE SECTION of 24 students)
PAGE 2 of 2!!!
Part 3: Aerobic Respiration
Students will do this part in table teams.
Materials:
Respiration apparatus (funnel attached to
stopper with glass tubing)
Ehrlenmeyer flasks (to fit respiration
apparatus) - 500 mL
Rubber stoppers (No. 7)
Large test tubes
Test tube racks
Phenol red dropper bottles (2%)
Cooked germinated pinto beans
Uncooked germinated pinto beans
Handful of dry pinto beans
Waste bucket for beans
Slotted spoons
Part 4: Anaerobic Respiration
Students will do this part in table teams.
Quantity:
1/table
3/table
2/table
6/table
1/table
1/table
1 large culture dish
1 large culture dish
For front table
2
2
Notes:
(Plus one for front teacher station)
(Plus one for front teacher station)
(Plus one for front teacher station)
Also used in Part 4 (Plus one for
front teacher station)
Also used in Part 4 (Plus one for
front teacher station)
To prep beans: soak for 1-2 nights,
covered in wet paper towels to
allow germination. On day of lab,
boil half the beans for 3-4 minutes
then cool down to room temp.
On side counter
One in each dish of beans
Materials:
Microspatulas for stirring in tubes
Water bath (37˚ C) with thermometer
Water bath (55˚ C) with thermometer
Ice bath
Yeast (in fridge)
Sugar: Lactose (with labeled scoop)
Sugar: Glucose (with labeled scoop)
Sugar: Sucrose (with labeled scoop)
Sugar: Dextrin (with labeled scoop)
Tubs for used test tubes
Balloons (9 inch)
Cloth measuring tapes
Rubber bands for test tubes (to keep
balloons on)
Biol 1: General Biology Spring 2015
Quantity:
1/table
1
1
1
2
2 culture dishes 2 culture dishes 2 culture dishes 2 culture dishes
1
5/table
1/table
5/table
Notes:
Side counter
Side counter
Side counter Side counter Side counter Side counter Side counter Side counter Side counter
CC-BY College of the Redwoods, Wendy Riggs
Lab 7: Mitosis and the Cell Cycle LAB PREPS
(This is the prep for ONE SECTION of 24 students)
Part 2: Mitosis Bead Simulations
Students will do this part in table teams.
Materials:
Chromosome bead sets
Part 3: Microscopic Mitosis
Students will do this part in table teams.
Materials:
Onion root tip mitosis slides
Whitefish mitosis slides
Part 4: Mitosis Bingo
Students will do this part in table teams.
Materials:
Slideshow of mitosis images
EXTRA SUPPLIES: Models
Students will do this part in table teams.
Materials:
Mitosis models
DNA models
Quantity:
Quantity:
1 box
1 box
Quantity:
1
Quantity:
2
Notes:
Notes:
14/2C
ZL6/113
Notes:
Email Riggs for a powerpoint file of mitosis
images, if you don't have one. But ANY images
of cells going through mitosis can be used.
Notes:
Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs
Lab 8: Meiosis and Gametogenesis LAB PREPS
(This is the prep for ONE SECTION of 24 students)
Part 1: Meiosis Bead Simulations
Students will do this part in table teams.
Materials:
Chromosome bead sets
Part 2: Independent Assortment
Students will do this part in table teams.
Materials:
Chromosome bead sets
Quantity:
Quantity:
Notes:
Notes:
Part 3: Mammalian Gametogenesis
Students will do this part in table teams.
Materials: Quantity: Notes:
Slides of ovaries 1 box HN 1/21
Slides of testes 1 box HM 1/2
EXTRA SUPPLIES: Models
Students will do this part in table teams.
Materials:
Mitosis models
DNA models
Quantity:
2
Notes:
Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs
Lab 9: Mendelian Genetics LAB PREPS
(This is the prep for ONE SECTION of 24 students)
Part 3: Probability
Students will do this part in table teams.
Materials: Quantity: Notes:
Pennies 2/pair
Part 4: Law of Independent Assortment
Students will do this part in table teams.
Materials: Quantity: Notes:
Genetic corn 9:3:3:1 box 1 box
Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs
Lab 10: Human Inheritance LAB PREPS
(This is the prep for ONE SECTION of 24 students)
Part 1: Human Traits Governed by Mendelian Genetics
Students will do this part in table teams.
Materials: Quantity: Notes:
PTC papers (just the blue packs) 1 pack/table
Part 2: Human Traits NOT Governed by Mendelian Genetics (BLOOD TYPING)
Students will do this part in pairs.
Materials: Quantity: Notes:
Blood type kits 1 box Place one bottle of synthetic blood serum and one
set of synthetic antisera on each bench.
Flat toothpicks 1 jar/table For stirring the blood and antibodies
Plastic wells 2/table
EXTRA SUPPLIES: Include the blood type key at the front table
Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs
Lab 11: DNA Structure and Function LAB PREPS
(This is the prep for ONE SECTION of 24 students)
Part 4: Wheat Germ Extraction
Students will do this part in pairs.
Materials:
Beaker (250 mL)
Graduated cylinder (10 mL)
Graduated cylinder (100 mL)
Serological pipettes (5 mL)
Pipettors (green)
Thermometers
Dawn dish detergent
Adolf's Meat Tenderizer (original
unseasoned)
Scoops for meat tenderizer
Raw untoasted wheat germ
Serological pipettes (5 mL)
Pipettors (green)
Digital balance
Weigh papers
1 M Sodium Bicarbonate
Waste buckets for wheat germ
Signes across sinks "No used
wheat germ disposal or you will be
destroyed!"
Glass DNA hooks
DNA model
Ice bath (large enough for 10
beakers)
Hot bath- 55˚C
Alcohol, 95% in freezer
Glass stirring rods
Wax pencils
Quantity:
1 /table
1/table
1/table
1/table
1/table
1/table
1/table
2 containers
2
2 containers
2
2
2
2 bottles?
2
6
1
20 mL/group
1/table
1/table
Notes:
Small personal container
On side counter
On side counter
On side counter
For sodium bicarbonate solution For sodium bicarbonate solution
Side counter Side counter
Each pair will need 5 mL
At instructor's station
At instructor's station
On side counter
On side counter- with thermometer
On side counter in ice bath
Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs
Lab 12: Diversity of Life LAB PREPS
(This is the prep for ONE SECTION of 24 students)
FIELD TRIP
NO LAB PREP
Materials: Quantity: Notes:
Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs
Lab 13: Evolution and Natural Selection LAB PREPS
(This is the prep for ONE SECTION of 24 students)
Part 1: Fork'n'beans
Students will do this part in table teams.
Materials:
Black beans
Pinto beans
Kidney beans (red beans
Navy beans (white beans)
Plastic forks
Plastic spoons
Plastic knives
Forceps
Cups or tubs to collect beans
Stopwatches
If outside:
Food trays
Meter sticks
Stakes
Yarn
If inside:
Trays with vermiculite
Coffee can strainers (with screen in
the bottom)
Quantity:
1 jar
1 jar
1 jar
1 jar
10
10
10
10
25
6
6
6
4/group
1 ball/group
2
1/table
1/table
Notes:
On side
On side
On side
On side
On side counter On side counter On side counter On side counter
On side counter
Most students have cell phones they can use...
Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs
Lab 14: Viruses, Bacteria and Epidemiology LAB PREPS
(This is the prep for ONE SECTION of 24 students)
Part 2: Bacteria and Archaea
Students will do this part on their own.
Materials:
Water fern
Razer blade
Cutting board
Glass slides
Coverslips
Part 3: Epidemiology
Students will do this part on their own.
Materials:
1 test tube rack
1 mL test tubes
Disposable 1mL pipettes
Phenol red dropper bottles
0.1 M NaOH
0.001 M HCl
Quantity:
not much
needed
Quantity:
24 (1/student)
6
Notes:
In Jeff's yard?
Notes:
In front
In front
1/table
Instructions:
1. Fill one test tube half full of 0.1M NaOH and put a small sharpie mark on the bottom of this test tube.
2. Fill 23 test tubes half full of 0.001 M HCl and leave them unmarked.
3. Put test tube rack filled with test tubes at the front of room, along with disposable pipettes.
Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs
Lab 15: Classification LAB PREPS
(This is the prep for ONE SECTION of 24 students)
Part 1: Dichotomous Key
Students will do this part in their table teams.
Materials:
6 critters specimens to classify
6-9 magnetic whiteboards
6-9 packs of magnetic
Caminalcules
Quantity:
1 set of 6/table
Notes:
Critters can vary---and all tables can have a
different assortment of critters.
Set out on the tables
Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs
Lab 16: Natural History Museum LAB PREPS
(This is the prep for ONE SECTION of 24 students)
FIELD TRIP to the NATURAL HISTORY MUSEUM
NO LAB PREP
Materials: Quantity: Notes:
Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs
Lab 17: Double Helix Movie Night LAB PREPS
(This is the prep for ONE SECTION of 24 students)
Materials: Quantity: Notes:
"Double Helix" DVD This movie is available in the CR library.
Biol 1: General Biology Spring 2015 CC-BY College of the Redwoods, Wendy Riggs
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