Piwi-interacting RNAs play a role in vitamin C-mediated ...
Proteins were then transferred to polyvinylidene fluoride membranes (Millipore, Burlington, MA, USA). The membranes were incubated in 5% bovine serum albumin for 1 h. To detect p16 expression, anti-p16 antibody (10883-1-AP, Proteintech, Rosemont, IL, USA, diluted 1:1000) was added to the membranes and incubated at 4°C overnight. ................
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