Centers for Disease Control and Prevention



Supporting MaterialMeasurement of Urinary Benzo[a]pyrene Tetrols and Their Relationship to Other PAH Metabolites and Cotinine in HumansDonald C. Hilton*, Debra A. Trinidad, Kendra Hubbard, Zheng Li, Andreas Sj?dinCenters for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA 30341*Corresponding AuthorDonald C. HiltonNational Center for Environmental HealthCenters for Disease Control and Prevention4770 Buford Highway NE, Atlanta, GA 30341, USAE-mail: DHilton@Tel. +1 770-488-4207 Fax: +1 770-488-0142Table S1 Demographics of Study Participants. SmokerNon-Smoker aMaleFemaleMaleFemaleCaucasianN1810814Age Range (years)18-5321-6319-4719-65Median Age (years)22.523.525.526AsianN------3Age Range (years)22-75Median Age (years)47HispanicN----1Age Range (years)59Median Age (years)59BlackN2------Age Range (years)23-23Median Age (years)23ALLN2010818Age Range (years)18-5321-6319-4719-75Median Age (years)2323.525.534.5a Four nonsmoking subjects were excluded from the data analysis because cotinine in urine indicated noncompliance (cotinine > 100 ng/mL).Table S2, Instrumental Conditions used for GC/MS analysis.Trace GCOvenRamp RateFinal TemperatureHold Time--70 °C3.5 min30 °C/min310 °C6.0 minPTV Base Temperature:70 °CSplit Flow:20 ml/minSplitless Time:2.00 minSolvent Valve Temperature:140 °CPressure (kPa)Time (min)Rate (°C/min)Temperature °CFlow (mL/min)Injection50.370100 (vent)Evaporation921.30.170Transfer2101.510.0310Clean14.010.0310100Column Flow:0.90 mL/min, Constant flowMS Transfer Line270 °CTriPlus AutosamplerSample Volume10.0 ?LAir Volume1.0 ?LPre-injection dwell time0.5 sPost-injection dwell time1.0 sViscosityViscousPre- and Post-injection washesSolventsA,B,-,- (A= EtOAc, B=Toluene)Solvent Cycles5Solvent Volume15 ?LTSQ MSIonizationNCICI Gas (Ammonia) flow2.2 mL/MinSource Temperature200 °CEmission Current 50 ?AElectron Energy-100 eVQ2 Gas (Argon)Pressure1.5Chrom Filter1.5MRM TransitionsCompounds MonitoredTransitionCollision Energy13C12 PCB 209 (recovery standard)509.50 0B[a]P tetrahydro tetrols446.00 → 255.2018B[a]P tetrahydro tetrols446.00a → 284.201813C6 B[a]P tetrahydro tetrols452.50 → 261.201813C6 B[a]P tetrahydro tetrols452.50a → 290.2018a Second transition from a given mass is set at 0.001 mass unit higher to work around an instrument setup limitation.Table S3, Concentrations of PAH Metabolites (pg/mL) after exposure to a barbequed meal.?Time to Urine Collection after Exposure (Hr.)0.5717.520.521.5Analyte1-Hydroxypyrene7027731380382382-Hydroxynaphthalene3869875251514371167723-Hydroxyfluorene1423274157971983BPT I-1<LOD0.980.910.35<LODBPT II-1<LOD0.970.650.15<LODTable S4, Comparison of PAH biomarker results with data from the 2011-2012 NHANES.(CDC, 2015) ?Current Study2011-2012 NHANESNonsmokerSmokerNonsmokerSmokerCompoundGM (pg/mL)GM (pg/mL)GM (pg/mL)GM (pg/mL)?1-Hydroxynaphthalene7935,0601,1909,590?2-Hydroxynaphthalene2,37014,1003,17012,4002-Hydroxyfluorene18371716411603-Hydroxyfluorene53.5351586059-Hydroxyfluorene2615132076091-Hydroxyphenanthrene1312011151982-Hydroxyphenanthrene78.5126541133-Hydroxyphenanthrene69.7159491404-Hydroxyphenanthrene25.141.118391-Hydroxypyrene10022383243REFERENCESCDC. Fourth Report on Human Exposure to Environmental Chemicals, Updated Tables (February, 2015), Centers for Disease Control and Prevention, Atlanta, 2015, accessed Jan 23, 2017. ................
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