Converting mass of a DNA band into moles:
Converting mass of a DNA band into moles:
First, decide if the band is composed of single or double-stranded DNA.
Then, chose the correct conversion factor:
Hyseq uses *:
Single-stranded: 330 grams per mole per base
330 (g/mol)/b
330 g / ( mol ( b )
Double-stranded: 660 grams per mole per base pair
660 (g/mol)/bp
660 g / ( mol ( bp )
Example problem:
Find the number of moles which compose a 1.5kb band whose mass was determined to be 120 ng (by gel comparison to standards).
(120 n g ) ( ( mol ( bp ) = 1.2E-4 nmol or 0.12 pmol
(1500 bp) ( 660 g )
*Other sources use slightly different factors:
323 for single and 610 for double; see:
To be truly accurate, determine ratio of each base then calculate conversion factor; see:
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