Pioneer Hi-Bred International, Inc.

1-

No.

201000204

Pioneer Hi-Bred International, Inc.

Whereas, THERE HAS BEEN PRESENTED TO THE

Secretary of Agriculture

An application requesting a certificate of protection for an alleged distinct variety of sexually reproduced, or tuber propagated plant, the name and description of which are contained in the application and exhibits, a copy of which is hereunto annexed and made a part hereof, and the various

requirements of LAW in such cases made and provided have been complied with, and the title thereto

is, from the records ofthe PLANT VARIETY PROTECTION OFFICE, in the applicant(s) indicated in the said

copy, and Whereas, upon due examination made, the said applicant(s) is (are) adjudged to be entitled to a certificate of plant variety protection under the LAW.

Now, therefore, this certificate of plant variety protection is to grant unto the said applicant(s) and the successors, heirs or assigns of the said applicant(s) for the term of TWENTY years from the date of this grant, subject to the payment of the required fees and periodic replenishment of viable basic seed of the

variety in a public repository as provided by LA W, the right to exclude others frOI1~selling the variety,

or offering it for sale, or reproducing it, or importing it, or exporting it, or conditioning it for propagation, or stocking it for any of the above purposes, or using it in producing a hybrid or different variety therefrom, to the extent provided by the PLANT VARIETY PROTECTION ACT. (84 STAT. 1542, AS

AMENDED, 7 U.S.C. 2321 ET SEQ.)

SOYBEAN

'RJS18003'

In Testimony Whereof, I have hereunto set my hand and caused the seal of the Plant Variety Protection Office to be affixed at the City of Washington, D. C. this twenty-seventh day of June, in the year two thousand and eleven.

Commissioner Plant Variety Protection Office Agricultural Marketing Service

f

REPRODUCE LOCALL Y.lnclude form number and date on all reproductions

U.S. DEPARTMENT OF AGRICULTURE AGRICUL TURAL MARKETING SERVICE SCIENCE AND TECHNOLOGY - PLANT VARIETY PROTECTION

OFFICE

APPLICATION FOR PLANT VARIETY PROTECTION CERTIFICATE (Instructbns 8nd Iflformafbn coJlectKJn buroen statement on reverse)

,. NAME OF OV\NER

Pioneer Hi-Bred International, Inc.

ADDRESS (Street and No, or RFD No, City. State, and ZIP Code, and COllntry)

7250 N.w. 62nd Avenue

P.O. Box 552 Johnston, IA 50131-0552

7. IF THE OWNER NAMED IS NOT A "PERSON". GIVE FORM OF ORGANIZATION (corporatIOn, partnarShlp, aSSOCl8tlOn, ale.)

Corporate

8. IF INCORPORATED. GIVE ST ATE OF INCORPORAT ION

Iowa

Form N> roved - OMB No. 0581 -0055

The 101k)Wlf)gstatemants are mada If) accorOanca w~h tha Pnvecy Aclof 1974 (0 U.S.C. 002a) ana lhe Paparwork ROOllctlOn Act (PRA) of 1990.

ApplcatJon IS reqUIred in order to determJfJ8 Jt a plant variety profecfKJn cert/icBte is to be issued (7 USC. 2421) InlormatJOn is held confdanlial IIntii certlJC8te is ISSIIOO(7 U. S.C. 2426)

2. TEMPOR"RY DESIGNATION OR EXPERIMENTAL NAME 3. VARIETY NAME

YB17E08

5. TELEPHONE (incJJJda area coda)

(515) 253-2197

6. FAA (include araa coda)

(515) 270-3928

9. DATE OF INCORPORATION

May 6, 1926

RJS18003

FOR OFFICIAL USE ONLY PVPO NUMBER

2 0 1 0 0'0 2 04

FILING DATE

2/17/,).010

10 NAME AND ADDRESS OF OV\NER REPRESENTATIVE(S)

Paul D. Koelling 7250 N.W. 62nd Avenue P.O. Box 552 Johnston, IA 50131-0552

TO SERVE IN THIS APPLICATION. (First perron listed w#lreceNe aU papars)

Pioneer Hi-Bred International, Inc. 7250 N.W. 62nd Avenue P.O. Box 552 Johnston, IA 50131-0552

FILING AND EXAMINATION FEES:

$ 1./1 3'~ 2. 00

R DATE E

1/7/';20(0

c CERTIFICATION FEE:

E

I

V

E

o DATE

11. TELEPHONE (Indude area coda)

(515) 253-2197

14 CROP KIND (Common Nama)

Soybean

15. GENUS AND SPECIES NAME OF CROP

Glycine max

12 FAX(lndude area code)

(515) 270-3928

16 FAMILY NAME (80tanical)

Fabaceae

17. IS THE VARIETY A FIRST GENERATION

o YES

NO

HYBRID?

13, E-MAIL

paul. koelling@

18 DOES THE VARIETY CONTAIN ANY TRANSGENES?

~

YES

D NO

(OPTIONAL)

IF SO. PLEASE GIVE THE ASSIGNED USDA-APHIS REFERENCE NUMBER FOR THE APPROVED PET ITION TO DEREGULATE THE GENET ICALL Y MODIFIED PLANT FOR

COMMERCIALIZATION.

19 CHECK APPROPRIATE BOX FOR EACH An ACHMENT SUBMITTED (Follow Instrucbons on reverse I

[X] a

exhibit A Ongin and Breeding History of the Variety

[Xl b

exhibit B Statemenl of Dlstindness

c.

exhibit C. ObJeC!Jve Description of Vanety

d

exhibit D. Additional Descnpbon of the Vanety (OptIOnal)

e

Exhibit E. Statement of the BaSIS ot the Owne~s Ownership

Exhioit F Declaration Regarding Deposit

[Xl g

Voucher Sample (3.000 Viable IIntrooled seeds or, for tllber propagatoo variates, verllCa&on

that &sslle cuI/lire will be depOSIled ana maintain&O In an approved putJ/Jc repository)

h

Filing and Examination Fee ($4.382). made payable 10 "Treasurer of the United

Slates" (Mail 10 the PlantVanety ProtectIOn Ollce)

20. DOES THE OWNER SPECIFY THAT SEED OF THIS VARIETY BE SOLD ONL Y AS A CLASS OF CERT IF lED SEED? (See Sec/IOn 83(a) of the Plant Varety Protectbn Act)

YES (If "yes", ammer Ilems 21 and 22 below) NO (If "no", go to Ilem 23) UNDECIDED 21, DOES THE OWNER SPECIFY THAT SEED OF THIS VARIETY BE LIMITED AS TO

NUMBER OF CLASSES?

YES

0 NO

0 IF YES. WHICH CLASSES?

FOUNDATION

0 REGISTERED

CERTIFIED

22. DOES THE OWNER SPECIFY

NUMBER OF GENERATIONS?

DYES

o NO

THAT SEED OF THIS VARIETY

BE LIMITED

AS TO

IF YES. SPECIFY THE NUMBER 1.2.3. elc. FOR EACH CLASS.

23. HAS THE VARIETY (INCLUDING ANY HARVESTED MATERIAL) OR A HYBRID PRODUCED

FROM THIS VARIETY BEEN SOLD. DISPOSED OF, TRANSFERRED. OR USED IN THE U. S. OR

OTHER COUNTRIES?

~

0 YES

NO

o FOUNDATION

0 REGISTERED

0 CERTIFIED

(If additbnal explBnaton is necessary, please use the space mdicated on the reverse.)

24. IS THE VARIETY OR ANY COMPONENT OF THE VARIETY PROTECTED BY INTELLECTUAL PROPERTY RIGHT (PLANT 8REEDER'S RIGHT OR PATENT)?

~

YES

D NO

IF YES. YOU MUST PROVIDE THE DATE OF FIRST SALE, DISPOSITION. TRANSFER. OR USE FOR EACH COUNTRY AND THE CIRCUMST ANCES. (Pleaoo lisa space indICa too on revarse)

IF YES. PLEASE GIVE COUNTRY. DATE OF FILING OR ISSUANCE AND ASSIGNED REFERENCE NUMBER. (Please uoo spece indicated on revaroo)

25. The owners declare IMt a viable sample of basic seed of Ihe vanety has been furnished with application and Will be replenished upon request in accordance ""th such regulations as may be applicable. or for a tuber propagated vanety a tissue culture ""II be deposited In a public repository ana maintained for the duration of the certificate.

The undersigned owner(s) is(are) the owner of thiS sexually reprOduced ortuber propagated plant vanety. ana belleve(s) thai tho vanety IS new. disbnd. unitol111. and stable as required in Sedlon 42. and is entitled to protectIOn under the prOl/iSIOnS of Section 42 or the Plant Variety Protection Act

Owner(s) is (are) Informed that false representatIon hereIn can Jeopardize protection and result In penalbes,

SIGNATURE OF OWNER

Paul D. Koelling

CAPAOTY OR TITLE

Process Improvement Manager

s. NAME (PI?? pnnl or type)

CAPAOTY OR TITLE

DATE

SY..a10 (07-01-Z109) oesgnea O'ftne Pl3ntVane~protectDn

omce

l

#201000204

RJS18003

(See reverse tor InStruetJons 8nd InforlTlitiOn cotJeclion 0tTrJen staremen()

GENERAL INSTRUCTlONS: To be effectively filed with the Plant Variety Protection Office (PVPO), ALL of the following items must be received in the PVPO: (1) Completed application form signed by the owner; (2) completed exhibits A, B, C, E, F; (3) for a tuber reproduced variety, verification that a viable (in the sense that it will reproduce an entire plant) tissue culture will be deposited and maintained in an approved public repository; and (4) payment by credit card or check drawn on a U.S. bank for $4,382 ($518 filing fee and $3,864 examination fee), payable to "Treasurer of the United States" (See Section 97.6 of/he Regulations and Rules of Practice). NEW: With the application for a seed reproduced variety or by direct deposit soon after filing, the applicant must provide at least 3,000 viable untreated seeds of the variety per se, and for a hybrid variety at least 3,000 untreated seeds of each line necessary to reproduce the variety. Partial applications will be held in the PVPO for not more than 90 days; then retumed to the applicant as un-filed. Mail application and other requirements to Plant Variety Protection Office, AMS, USDA, Room 401, NAL Building, 10301 Baltimore Avenue, Beltsville, MD 20705.2351. Retain one coPY for your files. All items on the face of the application are self explanatory unless noted below. Corrections on the application form and exhibits must be initialed and dated. DO NOT use masking materials to make corrections. If a certificate is allowed, you will be requested to send a payment by credit card or check payable to "Treasurer of the United Slates" in the amount of $768 for issuance of the certificate. Certificates will be issued to owner, not licensee or agent.

NOTES: It is the responsibility of the applicant/owner to keep the PVPO informed of any changes of address or change of ownership or assignment or owner's

representative during the life of the application/certificate. The fees for filing a change of address; owner's representative; ownership or assignment; or any modification of owner's name is specified in Section 97.175 of the regulations. (See Section 101 of the Act, and Sections 97.130, 97.131, 97.175(h) of/he Regulations and Rules of Practice.)

Plant Variety Protection Office

Telephone: (301) 504-5518

FAX: (301) 504-5291

General E.mall: PVPOmail@

Ho mepag e:

SPECIFIC INSTRUCTlONS: To avoid conflict with other variety names in use, the applicant must check the appropriate recognized authority and provtde evidence that the permanent name of the application variety (even if it is a parental, inbred line) has been cleared by the appropriate recognized authority before the Certificate of Protection is issued. For example, for agricultural and vegetable crops, contact: U.S. Department of Agriculture, Agricultural Marketing Service, Livestock and Seed Programs, Seed Regulatory and Testing Branch, 801 Summit Crossing Place, Suite C, Gastonia, North Carolina 28054-2193 Telephone: (704) 810-8870. .

ITEM 19a. Give:

(1) the genealogy, including public and commercial varieties, lines, or clones used, and the breeding method; (2) the details of subsequent stages of selection and multiplication; (3) evidence of uniformity and stability; and (4) the type and frequency of variants during reproduction and multiplication and state how these variants may be identified

19b. Give a summary of the variety's distinctness. Clearly state how this application variety may be distinguished from all other varieties in the same crop. If the new variety is most similar to one variety or a group of related varieties:

(1) identify these varieties and state all differences objectively; (2) attach replicated statistical data for characters expressed numerically and demonstrate that these are clear differences; and (3) submit, if helpful, seed and plant specimens or photographs (prints) of seed and plant comparisons which clearly indicate distinctness.

19c. Exhibit C forms are available from the PVPO Office for most crops; specify crop kind. Fill in Exhibit C (Objective Description of Variety) form as completely as possible to describe your variety.

19d. Optional additional characteristics and/or photographs. Describe any additional characteristics that cannot be accurately conveyed in Exhibit C. Use comparative varieties as is necessary to reveal more accurately the characteristics that are difficult to describe, such as plant habit, plant color, disease resistance, etc.

1ge. Section 52(5) of the Act requires applicants to furnish a statement of the basis of the applicant's ownership. An Exhibit E form is available from the PVPO.

20. If "Yes" is specified (seed of this variety be sold by variety name only, as a class of certified seed), the applicant MAY NOT reverse this affirmative decision after the variety has been sold and so labeled, the decision published, or the certificate issued. However, if "No" has been specified, the applicant may change the choice. (See Regulations and Rules of Practice, Section 97.103).

23. See Sections 41, 42, and 43 of the Act and Section 97.5 of/he regulations for eligibifity requirements.

24. See Section 55 of the Act for instructions on claiming the benefit of an earlier filing date.

22. CONTlNUED FROM FRONT (Please provide a statement as to the limitation and sequence ofgenerations that may be certified.)

23. CONTlNUED FROM FRONT (Please provide the date of first sale, disposition, transfer, or use for each country and the circumstances, if/he variety (including any harvested material) or a hybrid produced from this variety has been sold, disposed of, transferred, or used in the U. S. or other countries.) Transferred for experimental use In the United States. We are also reviewing our records to obtain details of activities Involving sale, disposition, transfer or use and will provide an amended statement with additional Information.

24. CONTlNUED FROM FRONT (Please give the country, date offiling or issuance, and assigned reference number, if the variety or any component of the variety is protected by intellectual property right (Plant Breeder's Right or Patent).) USVariety Patent Appl. No. 12/424,574 filed 16-Apr-09; US Patent No. 4,940,835

Issued 10-Jul-1990

Acx:orr1Jna 10 the Paperwork ReducUon Act or 19!KJ, an aaency may nol conducl or spensor, and a person IS not required 10 respOnd to a coQec/jon o! informaton unless ,t dISplays a val,d OMB control number. The vs/IQ OMB control number for thIS II1rormstJon coHeetion is 058141006 The tme requited to complete this information ooUectJon is estJmsted to eversge 14 hours per response, including the bme to, revievving instfUctbns, sesrchll'lQ existmo date sources, gatheflng 8nd mstnteining the dete needed, and completing BOOrevJ8Wing the coUectjon of information

The U.S, Department of Agrculture (USDA) prohibits discrimination in aI/its programs end Betivibes on the baS'1Sof mea, color, netK)nalorigJn, age, disability. and where apphcsbJe. sex. mental status, familisl status, patents! status, felK:}tJn, sexualoflentaton. ganele mto/maton, pohtJcal balets, fepflsal, or because all or pBrt of an II'1divK1uafs mpome IS derived from any pubic aSSIStance program (Not aN prohibited bases apply to all programs) Persons w,th disabi~/Jes who require allematNe means for communcatron of program informali:>n (Bra,Qe, large print. audiolape, etc) shout:! contect USDA's TARGET Center al (202) 720-2600 (voce and TOO). To file a complarnl or discflmrnati:>n, wrtle 10 USDA, Director, Office of Civil Rrghls, 14()0 Independence Avenue, S W, Washinalon, 0 C. 20250-9410, orc:aq (BOO) 795-3272 (voce) or (202) 720-o3B2 (TOO). USDA IS an equal opporlunrty provider and empbyer.

ST -470 (07-01.2)09) deSigned 'OtIlhe Plant vanetyoProrecoon OfTlce

#201000204

Exhibit A. Origin and Breeding History of the Variety

Soybean Variety RJS18003

Variety RJS 18003 evolved from a cross made in the winter of 2002/2003 in Puerto Rico with the following parentage:

Parentage = 91 B42/XB29K04

XB29K04 = 45217N901/ XB25P99

45217N901 = S2911/21066

21066 = M012557/90688A

= 90688A A4715/G40-3P9392* = XB25P99 93B82/91 013F2

= 91013F2 W30132-004/90753

W30132-004 = P9273/XB33B*A3733+RESN

= XB33B CM304BR/A3127

90753 = ST2250/90682

= 90682 P9342/G40-3P9341 *

(See Note 1)

* G40-3P9341 and G40-3P9392 are experimental lines with the Roundup Ready@ (403-2) gene.

Note 1: A3733+RESN was an F1 plant crossed to Pioneer experimental line XB33B. The F1 plant from the cross (XB33B*A3733+RESN) was crossed with Pioneer Variety 9273

Variety RJS18003 is an F3-derived line which was advanced to the F3 generation by modified single-seed descent. The F3:F4 progeny row of RJS18003 was grown in a plant row yield trial in Minnesota in the summer of 2004. Subsequently, RJS18003 has undergone five years of extensive testing and purification and has been observed by the breeder to be uniform and stable for all plant traits from generation to generation, with no evidence of variants. On the basis of yield, maturity, brown stem rot tolerance, Rps1 k Phytophthora resistance, soybean cyst nematode resistance, and resistance to Roundup@ branded herbicides; variety RJS18003 was assigned a commercial number.

Purification rows were grown in the summer of 2007 in Minnesota. A three tenth (0.30) acre increase was grown in Chile in the winter of 2007/2008. Twenty (20) acres of parent seed stock (foundation seed equivalent) were grown in the summer of 2008. Approximately 395 acres of seed stock and production seed were grown in the summer of 2009.

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#201000204

Exhibit B. Statement of Distinctness

Soybean Variety RJS 18003

Variety RJS18003 is most similar to Pioneer Variety 91Y80. Both varieties have light tawny pubescence, yellow seeds, with black hila, tan pods, high seed protein peroxidase activity, resistance to race 3 of the soybean cyst nematode, resistance to Phytophthora megasperma governed by the Rps1 k gene, and resistance to Roundup Ready@ herbicides. However, RJS18003 has purple flowers and dull seed coat luster; whereas 91Y80 has purple flowers and shiny seed coat luster.

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