N-Acetyl-L-Cysteine inhibition of virus-induced apoptosis.



N-Acetyl-L-Cysteine

Hazardous Substances Policy - Assessment

CHEMICAL HAZARD AND RISK ASSESSMENT

School of Biosciences

|Name of |Dr. C. Sweet | | |Assessment Number* |1863 |

|supervisor | | | | | |

| | Signature | | | |

|Assessor |Mr. E.W.A. Brydon | | |Date of Assessment |07.09.01 |

Signature

Notes A School COSHH form in Word is available on the School Server.

Available from the Health and Safety Unit.

Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.

Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.

Use a continuation sheet to expand any section of this form in hard copy version.

|1 | |LOCATION OF THE WORK ACTIVITY | |S105, S106B |

| | | | | |

|2 | |PERSONS WHO MAY BE AT RISK |

| | | | |

|List names where possible |All members of Dr. C. Sweets group present and future; currently Mr. E. W. A. Brydon, |

|Dr. S. J. Morris, Miss. I. Y. Pappworth, Miss K. Baluch, Dr. M. Kirby, Miss K.. Ball and Mr . N. Sharif, and future |

|M.Res. or 3rd year project students. |

|3 | |ACTIVITY ASSESSED | |N-Acetyl-L-Cysteine inhibition of virus-induced apoptosis. |

| | | | | |

|4 | |MATERIALS INVOLVED |Attach copies of data sheet(s) | |

|NAME |AMOUNT |HAZARD |RISK PHRASES |HAZDAT NO*** |BIOSCIENCESNO*** |

| |max/stored | | | | |

|N-Acetyl-L-Cysteine |10g |Caution |Caution: Avoid contact and | | |

| | | |inhalation. | | |

| | | | | | |

| | | | | | |

| | | | | | |

| | | | | | |

| | | | | | |

| | | | | | |

| | | | | | |

|5 | |INTENDED USE** |

| | | | |

|Give brief details and attach protocol/instructions |To be used as a tissue culture medium supplement, pre- and post |

|virus incubation, on cell monolayers in order to inhibit apoptosis. Weighed amounts will be diluted to |

|concentrations under 1M in tissue culture medium in the class 2 safety cabinet. |

| |

| |

|6 | |RISKS to HEALTH and SAFETY from INTENDED USE |

| | | | |

|From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially|

|at risk? |

|May cause: skin irritation, eye irritation, irritation to mucous membranes and the upper respiratory tract; |

|May be harmful if: absorbed through the skin, inhaled or swallowed. |

| |

|To the best of the manufacturers knowledge (Sigma-Aldrich) the chemical, physical and toxicological properties |

|have not been thoroughly investigated but the data sheet quotes no toxicological values below 400mg/kg. |

|OEL = MAK |

|Stability – stable |

|Incompatibilities – strong oxidising agents |

|Hazardous combustion or decomposition products – carbon monoxide, carbon dioxide, nitrogen oxides. |

|Hazardous polymerisation – will not occur. |

| |

| |

|7 | |CONCLUSIONS ABOUT RISKS |

| | | | |

|Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary? |

|Risk level is low and acceptable. |

| |

| |

| |

|8 | |CONTROL MEASURES |

| | | | |

|Additional to Good Chemical Practice | |

|Wear compatible chemically-resistant gloves and lab coat when weighing out in a closed microbalance. |

|Make dilutions in class 2 safety cabinet. |

| |

| |

| |

| |

| |

|9 | |INSTRUCTION/TRAINING |

| | | | |

|Specify course(s) and/or special arrangements. | |

|None. |

| |

| |

| |

|10 | |MONITORING |

| | | | |

|Performance of control measures, | |

|None |

| |

| |

|Personal exposure | |Health Surveillance | |

|None | |

| | |

|11 | |WASTE DISPOSAL PROCEDURE |

| See School Server for Approved Procedure Document on specific Chemical Waste Disposal. |

|Pour off aqueous solutions and autoclave with the rest of the tissue culture waste. |

| |

| |

| |

| |

| |

| |

| |

| |

| |

|12 | |REVIEW |

| | | | |

|Enter the date or circumstances for review of assessment (maximum review interval 5 years) | |

|5 years. |

| |

| |

| |

| |

| |

|13 | |EMERGENCY ACTION |

| | | | |

|TO CONTROL HAZARDS |To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc. |

|Max spillage equates to 10g therefore for spillage wear lab coat and rubber gloves. Sweep up, place in a bag |

|and hold for waste disposal avoiding the raising of dust. Ventilate area and wash spill site after material pick up. |

|For dissolved liquid spills absorb onto paper towelling and dispose of for autoclaving. Ventilate area and wash |

|spill site after material pick up. |

|TO PROTECT PERSONNEL |Evacuation, protection for personnel involved in clean-up, Special First Aid | |

|Avoid inhalation, contact with eyes, skin or clothing. |

|If swallowed, wash out mouth with water provided the person is conscious, call a physician. |

|If inhaled, remove to fresh air. If breathing becomes difficult call a physician. |

|In case of contact, immediately wash skin with soap and copious amounts of water. |

|In case of contact with eyes, flush with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating the eyelids with |

|fingers. Call a physician. |

|TO RENDER SITE OF EMERGENCY SAFE |Clean-up/decontamination | |

|See section 13. |

| |

| |

| |

| |

| CONTACT PHONE |

| |

10.10.00

* Prefix T is used for Teaching Assessment Number.

** Please include amount of chemicals used and how.

*** Hazdat No is the UNICOSHH datasheet report number.

Biosciences No is the Biosciences data sheet number.

UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.

BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.

................
................

In order to avoid copyright disputes, this page is only a partial summary.

Google Online Preview   Download