List of Lectures



INTD567/NEUR531 – Molecular and Cellular Neurobiology

Scope:

This course which will represent a “key” course for NGP (INTD/NEUR 567) is designed to introduce to graduate students the basic concepts and fundamental principles of advanced molecular and cellular neurobiology in the context of functional neural circuitry. It is divided into four blocks, each of which covers several major topics of an important sub-discipline in Molecular and Cellular Neuroscience. Each block consists of six 2-hour lectures and one conference in which students will be asked to present and discuss one or two recent scientific paper(s) relevant to topics covered in the lectures. The entire course is comprised of 23 lectures, 4 conferences and 2 exams. Students will earn 4 credits after successful completion of the lectures, conferences, and passing the required examination.

Course Designer’s Group:

Course directors: Jonah R. Chan, Ph.D. (jonahcha@usc.edu)

Li Zhang, Ph.D. (liizhang@usc.edu)

Block I director: Jonah R. Chan, Ph.D. (jonahcha@usc.edu)

Block II director: Le Ma, Ph.D. (le.ma@usc.edu)

Block III directors: Alapakkam Sampath, Ph.D. (asampath@usc.edu)

Block IV directors: Li Zhang, Ph.D. (liizhang@usc.edu)

Schedule and Location:

Lectures & Conference will be held from 10:00 AM to 12:00 PM, twice a week in the Zilkha Neurogenetic Institute, Herklotz Seminar Room, Room 112, 1501 San Pablo Street, Los Angeles, CA90033.

Course Materials:

Syllabus, lecture handouts, and papers for conferences will be posted in advance on the Blackboard: . Please check this site frequently for course information. Hard-copies of the lecture materials will not be provided.

Textbook for Background Reading:

Principles of Neural Science, fourth edition, By Eric R. Kandel, James H. Schwartz, Thomas M. Jessell, 2000, McGraw-Hill Professional. (ISBN 0838577016).

Conference Guidelines:

Written assignments

1. All students will be required to submit an approximately one-page evaluation of the paper that is to be discussed in each conference section. This is likely to be 3-4 paragraphs in length.

2. This evaluation should contain a brief summary of the goals of the paper, the results obtained and the conclusions drawn by the authors. Within this written evaluation, students should also identify what, in their own opinion, is the most important result(s) obtained in the paper and explain why they have this opinion. Finally, a brief critique of the paper should be presented. This critique could comment on various issues such as the validity of the authors’ conclusions (i.e. are the authors’ conclusions supported by the results presented), the soundness of the experimental approach, potential impact of the study and possible future experiments that would extend the results presented.

3. This written evaluation will be due at the beginning of each conference. No late evaluations will be accepted.

B. Paper presentation and discussion

1. One recent research paper (or two closely related) focusing on a major topic in each block will be selected from a leading scientific journal, and posted on the Blackboard: at least two weeks prior to the conference.

2. The whole class will be divided into several groups; each consists of 6–8 students and a faculty proctor. Student will receive a group assignment at least one week before each of the conferences.

3. During the conference, a single student will be assigned to present the Introduction, and one to present the Discussion of the paper. Those two students should come prepared with overhead figures to aid in their presentation. No students will be pre-assigned to specific figures. Rather, students will be chosen at random to present individual figures. Conference proctors will be responsible for providing overhead transparencies of the figures. Thus, if you are not assigned to present the Introduction or Discussion, you should be prepared to discuss any one of the figures presented in the paper.

4. At the end of the course, grades for written evaluations will be combined with grades for oral presentation (equally weighted) for calculation of the final grade for the conference.

Examination:

There will be two exams at the end of the second and fourth blocks. They will be 1.5-hour, closed-book written tests. The format will be short answer questions that emphasize problem solving rather than fact memorizing.  Multiple choice questions will not be used. No make-ups will be given for the exam. The final grade will be comprised of the examinations (80%) and conference (20%) [Written assignments=10%; paper presentation and discussion=10%].

LIST OF LECTURES & SUGGESTED INSTRUCTORS

Block I. Cellular biology of the neural circuit

|# |LECTURE TITLE |SUGGESTED INSTRUCTORS |

|Lecture 1 |Introduction into the organization of the nervous system and objectives for |Jonah Chan |

| |course. |Aug 25th |

| |-Chapt 2-3, Principles of Neural Science | |

| |-Albright TD, Jessell TM, Kandell ER, Posner MI. Progress in the neural sciences | |

| |in the the century after Cajal (and the mysteries that remain). Ann N Y Acad Sci. | |

| |2001 Apr;929:11-40. | |

| |-Albright TD, Jessell TM, Kandell ER, Posner MI. Neural science: a century of | |

| |progress and the mysteries that remain. Cell. 2000 Feb 18;100 Suppl:S1-55. | |

|Lecture 2 |General aspects of transcriptional and translational regulation in the neuron, |Derek Sieburth |

| |including axonal transport. |Aug 27th |

| |-Chapter 4, Principles of Neural Science | |

| |-Willis DE, Twiss JL. The evolving roles of axonally synthesized proteins in | |

| |regeneration. Curr Opin Neurobiol. 2006 Feb;16(1):111-8. | |

| |-Hirokawa N, Takemura R. Molecular motors and mechanisms of directional transport | |

| |in neurons. Nat Rev Neurosci. 2005 Mar;6(3):201-14. | |

| |-Piper M, Holt C. RNA translation in axons. Annu Rev Cell Dev Biol. | |

| |2004;20:505-23. | |

|Lecture 3 |Cell biology of the neuron (I) Cytoskeleton, molecular motors and cell polarity. |Jonah Chan |

| |-Chapter 4, Principles of Neural Science |Sept 3rd |

| |-Arimura N, Kaibuchi K. Neuronal polarity: from extracellular signals to | |

| |intracellular mechanisms. Nat Rev Neurosci. 2007 Mar;8(3):194-205. | |

| |-Nakata T, Hirokawa N. Neuronal polarity and the kinesin superfamily proteins. Sci| |

| |STKE. 2007 Feb 6;2007(372):pe6. | |

| |-Wiggin GR, Fawcett JP, Pawson T. Polarity proteins in axon specification and | |

| |synaptogenesis. Dev Cell. 2005 Jun;8(6):803-16. | |

|Lecture 4 |Cell biology of the neuron (II) Membrane dynamics: specialized membrane domains |Jeannie Chen |

| |(lipid rafts/caveoli), trafficking, secretion and endocytosis. |Sept 8th |

|Lecture 5 |Neuronal Stem Cells |Wange Lu |

| | |Sept 10th |

|Conference 1 |Tentative papers for presentation and group discussion: |Jonah Chan and Li Zhang |

| | |Sept 15th |

| |Paper 1: | |

| |Shelly M, Cancedda L, Heilshorn S, Sumbre G, Poo MM. LKB1/STRAD promotes axon | |

| |initiation during neuronal polarization. Cell 129(3):565-77 (2007). | |

| | | |

| |Paper 2: | |

| |Barnes AP, Lilley BN, Pan YA, Plummer LJ, Powell AW, Raines AN, Sanes JR, Polleux | |

| |F. LKB1 and SAD kinases define a pathway required for the polarization of cortical| |

| |neurons. Cell 129(3):549-63 (2007). | |

Block II. Development of neural circuitry

|# |LECTURE TITLE |SUGGESTED INSTRUCTORS |

|Lecture 1 |Neural induction and regionalization |Le Ma |

| |- Topics: neural inducer, Hox genes, A/P and D/V polarity |Sept 17th |

| |- Principles of neurosciences 3rd edition, chapter. 21 | |

| |- De Robertis and Kuroda, Dorsal-ventral patterning and neural induction in | |

| |Xenopus embryos. Annu Rev Cell Dev Biol. 2004;20:285-308. | |

| |- Lee and Jessell, The specification of dorsal cell fates in the vertebrate | |

| |central nervous system. Annu Rev Neurosci. 1999;22:261-94 | |

|Lecture 2 |Neuronal migration and differentiation, |Le Ma |

| |- Topics: neural progenitors, neurogenesis, asymmetry cell division,radial glial,|Sept 22nd |

| |migration | |

| |- Principles of neurosciences 3rd edition, chapter. 57-58 | |

| |- Hatten, 2002, New directions in neuronal migration. Science. 2002 Sep | |

| |6;297(5587):1660-3 | |

| |- Zhao, Deng, and Gage, Mechanisms and functional implications of adult | |

| |neurogenesis. Cell. 2008 Feb 22;132(4):645-60 | |

| |- Zhong Diversifying neural cells through order of birth and asymmetry of | |

| |division. Neuron. 2003 Jan 9;37(1):11-4 | |

|Lecture 3 |Axon guidance and dendritic development |Le Ma |

| |- topics: growth cones, guide post cell, attractive/repulsive cues, dendritic |Sept 24th |

| |arbor | |

| |- Principles of neurosciences 3rd edition, chapter. 58 | |

| |- Dickson, 2002,Molecular mechanisms of axon guidance Science. 2002 Dec | |

| |6;298(5600):1959-64 | |

| |- Parrish JZ, Emoto K, Kim MD, Jan YN. Mechanisms that regulate establishment, | |

| |maintenance, and remodeling of dendritic fields. Annu Rev Neurosci. | |

| |2007;30:399-423 | |

|Lecture 4 |Target selection and axon elimination |Le Ma |

| |- topics: retinal map, olfactory map, chemoaffinity theory, pruning |Sept 29th |

| |- Principles of neurosciences 3rd edition, chapter 58 | |

| |- Luo and Flanagan, 2007,Development of continuous and discrete neural maps | |

| |Neuron. 2007 Oct 25;56(2):284-300 | |

|Lecture 5 |Neural-glial interactions and nerve regeneration |Jonah Chan |

| |- topics: myelination, myelin-derived growth inhibition |Oct 1st |

| |- Principles of neurosciences 3rd edition, chapter 18 ? | |

| |- Yiu and He, 2007,Glial inhibition of CNS axon regeneration. Nat Rev Neurosci. | |

| |2006 Aug;7(8):617-27 | |

|Lecture 6 |Synapse formation and maturation |Derek Sieburth |

| |- topics: neuromuscular junction, dendritic spines, genetic models |Oct 6th |

| |- Principles of neurosciences 3rd edition, chapter 59 | |

| |- Goda and Davis, Mechanisms of synapse assembly and disassembly. Neuron. 2003 | |

| |Oct 9;40(2):243-64 | |

| |- Cohen-Cory, The developing synapse: construction and modulation of synaptic | |

| |structures and circuits. Science. 2002 Oct 25;298(5594):770-6 | |

|Conference 2 |Tentative papers for presentation and group discussion: |Le Ma |

| | |Oct 8th |

| |- Yamagada and Sanes, Dscam and Sidekick proteins direct lamina-specific synaptic| |

| |connections in vertebrate retina. Nature. 2008, 451(7177):465-9 | |

| |- Fuerst, et al, Neurite arborization and mosaic spacing in the mouse retina | |

| |require DSCAM. Nature. 2008, 451(7177):470-4. | |

|Mid Term Exam |1.5 hour, closed-book exam |Oct 13th |

Block III. Intracellular signaling in neural circuitry

|# |LECTURE TITLE |SUGGESTED INSTRUCTORS |

|Lecture 1 |Sensory Systems (I): Vision and Non-Visual Light Reception |A.P. Sampath |

| |-Chapt 21, 26, Principles of Neural Science |Oct 15th |

| |-Burns, M.E., and Baylor, D.A. (2001) Activation, deactivation and adaptation in | |

| |vertebrate photoreceptor cells. Ann Rev Neurosci 24: 779-805 | |

|Lecture 2 |Sensory Systems (II): Olfaction, & Taste |A.P. Sampath |

| |-Chapt 32, Principles of Neural Science |Oct 20th |

| |-Axel, R (2004) Scents and sensibility: a molecular logic of olfactory perception,| |

| |Nobel Lecture | |

| |- Roper, S.D.(2007) Signal transduction and information processing in mammalian | |

| |taste buds. Pflugers Arch 454: 759-776 | |

|Lecture 3 |Sensory Systems (III): Pain and Nociception |David McKemy |

| |-Chapt XX., Principles of Neural Science |Oct 22nd |

| |- McKemy, D.D. (2007) Temperature sensing across species. Pflugers Arch 454: | |

| |777-791. | |

| |- Julius, D. and Basbaum, A.I. (2001) Molecular mechanisms of nociception. Nature | |

| |413: 203-210 | |

|Lecture 4 |Sensory Systems (IV): Somatosensation and Auditory Transduction |A.P. Sampath |

| |-Chapt 30, 31, Principles of Neural Science |Oct 27th |

| |- Grant, L., and Fuchs, P.A. (2007) Auditory transduction in the mouse. Pflugers | |

| |Arch 454: 793-804. | |

| |-Lumpkin, E.A., and Caterina, M.J. (2007) Mechanisms of sensory transduction in | |

| |the skin. Nature: 858-865 | |

|Lecture 5 |Neurotrophic factors and receptors |Jonah Chan |

| |-Huang EJ, Reichardt LF. Trk Receptors: Roles in Neuronal Signal Transduction. |Oct 29th |

| |Annu Rev Biochem 2003 72:609-642. | |

| |-Huang EJ, Reichardt LF. Neurotrophins: Roles in Neuronal Development and | |

| |Function. Annu Rev Neurosci 2001 24:677-736. | |

|Lecture 6 |Synaptic plasticity |H.W. Tao |

| | |Nov 3rd |

|Conference 2 |Tentative papers for presentation and group discussion: |A.P. Sampath and H.W. Tao |

| | |Nov 5th |

| |Paper 1: Zhang, Y, Hoon, M.A, Chandrasekhar, J., Mueller, Cook B., Wu, D., Zuker, | |

| |C.S., and Ryba, N.J. (2003) Coding of sweet, bitter, and umami tastes: different | |

| |receptor cells sharing similar signaling pathways. Cell 112: 293-301. | |

| | | |

| |Paper 2: | |

Block IV. The functioning neural circuit

|LECTURE # |LECTURE TITLE |SUGGESTED INSTRUCTORS |

|Lecture 1 |Neuronal excitability and voltage-gated ion channels |Bob Farley |

| |Electrotonic properties of axons and dendrites (cable theory); membrane permeation|Nov 10th |

| |(GHK equations), Hodgkin-Huxley papers, axonal Na+ and K+ currents, complete model| |

| |of active membrane, K+ channels and gating current | |

| | | |

| |-chapt. 6-8, principles 3rd edition | |

|Lecture 2 |Presynaptic release mechanism |Bob Chow |

| |Biophysics of Ca2+ signaling; Ca2+ channels; Neurotransmitters; Exocytosis |Nov 12th |

| | | |

| |- chapt. 13-15, principles 3rd edition | |

|Lecture 3 |Ionotropic and Metabotropic receptors |A.P.Sampath |

| |Synaptic transmission in excitatory & inhibitory synapses; glutamate & GABA |Nov 17th |

| |receptors; molecular and structural basis for receptor function | |

| | | |

| |-Chapt 13, 15, Principles of Neural Science | |

| |- Isaac, J.T. Ashby M., and McBain C.J. (2007) The role of GluR2 subunit in AMPA | |

| |receptor function and synaptic plasticity. Neuron 859-871. | |

| |- Kim D.S., Palmiter, R.D. (2008) Interaction of dopamine and adenosine receptor | |

| |function in behavior: studies with dopamine- deficient mice. Front Biosci 13: | |

| |2311-2318 | |

|Lecture 4 |Dendritic integration |Zhang/Mel |

| |Text: Dendrites, Second Edition (Sept 07), Edited by Greg Stuart, Nelson Spruston,|Nov 19th |

| |and Michael Häusser, | |

|Lecture 5 |Introduction of neural circuitry |Li Zhang |

| |Questions, principles and approaches |Nov 24th |

|Lecture 6 |Functional circuitry for cortical receptive fields |Li Zhang |

| |Recent findings from sensory cortices |Dec 1st |

| | | |

| |- part.5, principles 3rd edition | |

|Conference 4 | |Zhang/ Tao |

| |Paper presentation and discussion: |Dec 3rd |

| | | |

| |Discussion on the circuitry mechanism of simple cell properties in the visual | |

| |cortex: thalamocorticals vs. intracortical | |

|Final Exam |A 1.5-hour written exam |TBA |

................
................

In order to avoid copyright disputes, this page is only a partial summary.

Google Online Preview   Download