An optogenetic gene expression system with rapid activation and ...
[Pages:9]article
published online: 12 january 2014 | doi: 10.1038/nchembio.1430
? 2014 Nature America, Inc. All rights reserved.
An optogenetic gene expression system with rapid activation and deactivation kinetics
Laura B Motta-Mena1,2, Anna Reade3,4, Michael J Mallory5, Spencer Glantz6, Orion D Weiner3,4, Kristen W Lynch5 & Kevin H Gardner1,2,7*
Optogenetic gene expression systems can control transcription with spatial and temporal detail unequaled with traditional inducible promoter systems. However, current eukaryotic light-gated transcription systems are limited by toxicity, dynamic range or slow activation and deactivation. Here we present an optogenetic gene expression system that addresses these shortcomings and demonstrate its broad utility. Our approach uses an engineered version of EL222, a bacterial light-oxygenvoltage protein that binds DNA when illuminated with blue light. The system has a large (>100-fold) dynamic range of protein expression, rapid activation ( ................
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