Cell Culture Protocols and Notes

One other modification is that once you add the citric saline you typically do not need to place the dishes in the incubator to help the cells detach. Transfecting Cells . Transfections using Lipofectamine 2000. Lipofectamine 2000 is used here to introduce siRNA or plasmid DNA by a process called lipofection. This process makes the cell membrane porous and allows the DNA to cross into the ... ................
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