A few peptides, mostly those containing the helix I of ...



Supplementary informationResults from the epitope definition stageSection 1. Further sample informationAll ME patients were from the Gottfries Clinic in Gothenburg. The Test (n=69; during 2008-2009) and Evaluation (n=61; during 2010-2011) sets were diagnosed according to the same criteria, but the patients were not the same. The main diagnosis of all 61 in the Evaluation set was ME. The Test set included both samples from ME and non-ME patients,where 69 had ME with of without FM and IBS, 4 had FM and 3 had FM+IBS. Section 2. Further information regarding antigens for the suspension array Synthetic peptidesHuman HSP60 peptides were chosen from the published sequences of human HSP60 (GenBank id NP_955472), Chlamydia pneumoniae HSP60 (Genbank Id NP_224342) and Mycoplasma penetrans HSP60, also named GroL (GenBank ID NP_757486). All peptides were 30-mers and had a three-carbon polyethylene glycol spacer coupled at the amino end. The spacer started with a primary amino group. Two overlapping series of Chlamydia peptides were tested, one with a three-carbon and one with a six-carbon polyethylene glycol spacer. The peptides overlapped by 15 amino acids. The peptides were synthesized by the coauthor dr Rüdiger Pipkorn. Lyophilized peptides were dissolved in sterile phosphate-buffered saline (PBS), pH 7. The dissolution sometimes had to be facilitated by warming the solution to 37°C overnight on a shaker or by sonication for 5 min. Highly hydrophobic peptides were dissolved in 10 to 50% (vol/vol) dimethyl sulfoxide (DMSO) (Sigma D2650). Initially, 38 overlapping peptides of HSP60 were synthesized, (Supplementary table ST1).In initial experiments, two human HSP60 peptides G15 and G20, gave significant IgG reactions. To better localize the epitopes, peptides overlapping G15 (G15a, G15b, G15c, G15d, G15e, and G15f) and G20 (G20a, G20b, G20c, G20d, G20e, and G20f), systematically shifted three amino acids before and after G15 and G20, respectively, were synthesized (Supplementary table ST2). A human HSP60 peptide (G20c) showed a higher antibody binding activity than the human HSP60 G20 peptide. Twentysix new peptides were therefore designed after an alignment of Human HSP60 G20c analogs with HSP60 of many microbes, spanning much of the HSP60 variation. The prokaryotes Orientia tsutsugamushi, Neoehrlichia mikurensis, Wolbachia, Legionella pneumophila, Borrelia garinii, Bartonella henselae, Tropheryma whipplei, Rickettsia bellii, Anaplasma phagocytophilium, Burkholderia multivorans, Escherichia coli, Chlamydophila pneumoniae , Staphylococcus aureus, Mycobacterium tuberculosis, Mycoplasma penetrans, Listeria monocytogenes, Streptococcus pneumoniae, Treponema pallidum,and the eukaryotes Leishmania (strain Friedlin), Schistosoma mansoni , Plasmodium falciparum, Leptospira interrogans, Giardia lamblia, Cryptosporidium parvum, and Entamoeba histolytica were included (cf. Supplementary table ST3). Further, 36 overlapping peptides from Mycoplasma penetrans HSP60 (Supplementary table ST4), and two sets of 36 overlapping Chlamydia pneumoniae HSP60 peptides, with a three and six carbon polyethylene glycol spacer, respectively, were synthesized (Supplementary table ST5).E. coli HSP60 (GroEL) selectively binds tryptophan-rich synthetic peptides (here termed “Strongly Binding Peptide, SBP”, defined from panning with E. coli HSP60 of a peptide library ADDIN EN.CITE <EndNote><Cite><Author>Chen</Author><Year>1999</Year><RecNum>5784</RecNum><DisplayText>(<style face="italic">1</style>)</DisplayText><record><rec-number>5784</rec-number><foreign-keys><key app="EN" db-id="f29rxsde60p9fsev5d9v9relserrtavztsvz">5784</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Chen, L.</author><author>Sigler, P. B.</author></authors></contributors><auth-address>Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511, USA.</auth-address><titles><title>The crystal structure of a GroEL/peptide complex: plasticity as a basis for substrate diversity</title><secondary-title>Cell</secondary-title><alt-title>Cell</alt-title></titles><periodical><full-title>Cell</full-title></periodical><alt-periodical><full-title>Cell</full-title></alt-periodical><pages>757-68</pages><volume>99</volume><number>7</number><edition>2000/01/05</edition><keywords><keyword>Chaperonin 10/*chemistry</keyword><keyword>Chaperonin 60/*chemistry</keyword><keyword>Escherichia coli/*chemistry</keyword><keyword>Fluorescence Polarization</keyword><keyword>Magnetic Resonance Spectroscopy</keyword><keyword>Models, Molecular</keyword><keyword>Molecular Sequence Data</keyword><keyword>Protein Binding</keyword><keyword>Protein Conformation</keyword><keyword>Protein Structure, Tertiary</keyword><keyword>X-Ray Diffraction</keyword></keywords><dates><year>1999</year><pub-dates><date>Dec 23</date></pub-dates></dates><isbn>0092-8674 (Print)&#xD;0092-8674 (Linking)</isbn><accession-num>10619429</accession-num><work-type>Research Support, Non-U.S. Gov&apos;t&#xD;Research Support, U.S. Gov&apos;t, P.H.S.</work-type><urls><related-urls><url>;(1). The binding of such a peptide (here called SBP1), defined by crystallography, was close to the apical domain helix I. Binding of SBPs is therefore a way of demonstrating chaperonin activity. We synthesized five SBP dodecamers defined in (Supplementary table ST6). They were biotinylated at the amino terminus. The biotin was joined to the SBP via a 6 carbon polyethylene glycol spacer, the same arrangement as in the overlapping long spacer chlamydia HSP60 30-mer peptides. Table ST1. The amino acid sequences of overlapping peptides of intact human HSP60 (GenBank id NP_955472).Turqoise background: Cross reactive epitopes (between Mycobacteria, Chlamydia, E.coli and Human HSP60) defined by Perschinka et al PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5QZXJzY2hpbmthPC9BdXRob3I+PFllYXI+MjAwMzwvWWVh

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ADDIN EN.CITE.DATA (2). Gray background: Sequence homologous with the Porphyromonas gingivalis peptide of Jeong et al ADDIN EN.CITE <EndNote><Cite><Author>Jeong</Author><Year>2012</Year><RecNum>3960</RecNum><DisplayText>(<style face="italic">3</style>)</DisplayText><record><rec-number>3960</rec-number><foreign-keys><key app="EN" db-id="f29rxsde60p9fsev5d9v9relserrtavztsvz">3960</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Jeong, E.</author><author>Lee, J. Y.</author><author>Kim, S. J.</author><author>Choi, J.</author></authors></contributors><auth-address>Department of Molecular Biology, College of Natural Sciences, Pusan National University, Pusan, South Korea Department of Periodontology, School of Dentistry, Pusan National University, Yangsan City, South Korea.</auth-address><titles><title>Predominant immunoreactivity of Porphyromonas gingivalis heat shock protein in autoimmune diseases</title><secondary-title>Journal of periodontal research</secondary-title><alt-title>J Periodontal Res</alt-title></titles><periodical><full-title>Journal of periodontal research</full-title><abbr-1>J Periodontal Res</abbr-1></periodical><alt-periodical><full-title>Journal of periodontal research</full-title><abbr-1>J Periodontal Res</abbr-1></alt-periodical><pages>811-6</pages><volume>47</volume><number>6</number><edition>2012/07/21</edition><dates><year>2012</year><pub-dates><date>Dec</date></pub-dates></dates><isbn>1600-0765 (Electronic)&#xD;0022-3484 (Linking)</isbn><accession-num>22812430</accession-num><urls><related-urls><url>;(3), antibodies to which were more frequent in chronic periodontitis, atherosclerosis, type 2 diabetes mellitus and rheumatoid arthritis. Another peptide sequence, antibodies to which correlated with cardiovascular disease, Okada et al PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5Pa2FkYTwvQXV0aG9yPjxZZWFyPjIwMDc8L1llYXI+PFJl

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ADDIN EN.CITE.DATA (5). The bold V in position 73 is mutated in Hereditary Spastic Paraplegia SPG13, labelled as position 72 in Hansen et al PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5IYW5zZW48L0F1dGhvcj48WWVhcj4yMDAyPC9ZZWFyPjxS

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ADDIN EN.CITE.DATA (6). The underlined letters shows the autoepitopes to which antibodies were boosted by Coxsackievirus A9 infection, H?rk?nen et al. PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5IYXJrb25lbjwvQXV0aG9yPjxZZWFyPjIwMDA8L1llYXI+

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ADDIN EN.CITE.DATA (7).A detailed discussion of peptidic HSP60 epitopes is given below (“Further details on HSP60 epitopes”). Peptide name Peptide sequence G1Human HSP60 1-30 MLRLPTVFRQMRPVSRVLAPHLTRAYAKDVG2Human HSP60 16-45 RVLAPHLTRAYAKDVKFGADARALMLQGVDG3Human HSP60 31-60KFGADARALMLQGVDLLADAVAVTMGPKGR G4Human HSP60 46-75 LLADAVAVTMGPKGRTVIIEQSWGSPKVTKG5Human HSP60 61-90TVIIEQSWGSPKVTKDGVTVAKSIDLKDKYG6Human HSP60 76-105DGVTVAKSIDLKDKYKNIGAKLVQDVANNTG7Human HSP60 91-120KNIGAKLVQDVANNTNEEAGDGTTTATVLAG8Human HSP60106-135NEEAGDGTTTATVLARSIAKEGFEKISKGAG9Human HSP60121-150RSIAKEGFEKISKGANPVEIRRGVMLAVDAG10Human HSP60136-165NPVEIRRGVMLAVDAVIAELKKQSKPVTTPG11Human HSP60151-180VIAELKKQSKPVTTPEEIAQVATISANGDKG12Human HSP60166-195EEIAQVATISANGDKEIGNIISDAMKKVGRG13Human HSP60181-210EIGNIISDAMKKVGRKGVITVKDGKTLNDEG14Human HSP60196-225KGVITVKDGKTLNDELEIIEGMKFDRGYISG15Human HSP60211-240LEIIEGMKFDRGYISPYFINTSKGQKCEFQG16Human HSP60226-255PYFINTSKGQKCEFQDAYVLLSEKKISSIQG17Human HSP60241-270DAYVLLSEKKISSIQSIVPALEIANAHRKPG18Human GroEL256-285SIVPALEIANAHRKPLVIIAEDVDGEALSTG19Human GroEL271-300LVIIAEDVDGEALSTLVLNRLKVGLQVVAVG20Human GroEL286-315LVLNRLKVGLQVVAVKAPGFGDNRKNQLKDG21Human GroEL301-330KAPGFGDNRKNQLKDMAIATGGAVFGEEGLG22Human GroEL316-345MAIATGGAVFGEEGLTLNLEDVQPHDLGKVG23Human GroEL331-360TLNLEDVQPHDLGKVGEVIVTKDDAMLLKGG24Human GroEL346-375GEVIVTKDDAMLLKGKGDKAQIEKRIQEIIG25Human GroEL361-390KGDKAQIEKRIQEIIEQLDVTTSEYEKEKLG26Human GroEL376-405EQLDVTTSEYEKEKLNERLAKLSDGVAVLKG27Human GroEL391-420NERLAKLSDGVAVLKVGGTSDVEVNEKKDRG28Human GroEL406-435VGGTSDVEVNEKKDRVTDALNATRAAVEEGG29Human GroEL421-450VTDALNATRAAVEEGIVLGGGCALLRCIPAG30Human GroEL436-465IVLGGGCALLRCIPALDSLTPANEDQKIGIG31Human GroEL451-480LDSLTPANEDQKIGIEIIKRTLKIPAMTIAG32Human GroEL466-495EIIKRTLKIPAMTIAKNAGVEGSLIVEKIMG33Human GroEL481-510KNAGVEGSLIVEKIMQSSSEVGYDAMAGDFG34Human GroEL496-525QSSSEVGYDAMAGDFVNMVEKGIIDPTKVVG35Human GroEL511-540VNMVEKGIIDPTKVVRTALLDAAGVASLLTG36Human GroEL526-555RTALLDAAGVASLLTTAEVVVTEIPKEEKDG37Human GroEL541-570TAEVVVTEIPKEEKDPGMGAMGGMGGGMGGG38Human GroEL556-573PGMGAMGGMGGGMGGGMFTable ST2. The amino acid sequences of overlapping peptides of fragments around human HSP60 G15 (aa211-240) and human HSP60 G20 (aa286-320), shown in red. Peptide name Peptide sequence G15aHuman GroEL202-231KDGKTLNDELEIIEGMKFDRGYISPYFINTG15bHuman GroEL205-234KTLNDELEIIEGMKFDRGYISPYFINTSKGG15cHuman GroEL208-237NDELEIIEGMKFDRGYISPYFINTSKGQKCG15Human GroEL211-240LEIIEGMKFDRGYISPYFINTSKGQKCEFQG15dHuman GroEL214-243IEGMKFDRGYISPYFINTSKGQKCEFQDAYG15eHuman GroEL217-246MKFDRGYISPYFINTSKGQKCEFQDAYVLLG15fHuman GroEL220-249DRGYISPYFINTSKGQKCEFQDAYVLLSEKG20aHuman GroEL277-306DVDGEALSTLVLNRLKVGLQVVAVKAPGFGG20bHuman GroEL280-309GEALSTLVLNRLKVGLQVVAVKAPGFGDNRG20cHuman GroEL283-312LSTLVLNRLKVGLQVVAVKAPGFGDNRKNQ G20Human GroEL286-320LVLNRLKVGLQVVAVKAPGFGDNRKNQLKDG20dHuman GroEL289-318NRLKVGLQVVAVKAPGFGDNRKNQLKDMAIG20eHuman GroEL292-321KVGLQVVAVKAPGFGDNRKNQLKDMAIATGG20fHuman GroEL295-324LQVVAVKAPGFGDNRKNQLKDMAIATGGAVTable ST3. The amino acid sequences of GroEL of many microbes which are homologs of Human HSP60 (283-312) (Here referred to as “G20c homologs”); Peptide names contain the respective GenBank identity numbers)Peptide namePeptide sequenceGroEL_orientia_tsutsugamushi_CH60_ORITS 258-287 LTALILNNLKGSIKVVAVKAPGFGDRKKEMGroEL5_neoehrlichia_mikurensis_FJ966359 259-288 LSTLVLNKLRGGLHVAAVKAPGFGDRRKDMGroEL_Wolbachia_YP_198181 261-290 LSTLVINKLRGGLKVTAVKAPGFGDRRKEMGroEL_Legionella_YP_126086 258-287 LATLVVNNMRGIVKVCAVKAPGFGDRRKAMGroEL_Borrelia_garinii_YP_073092 258-287 LAALVLNSVRGALKVCAIKSPGFGDRRKAMGroEL_Bartonella_henselae_YP_034075 259-288 LATLVVNKLRGGLKIAAVKAPGFGDRRKAMGroEL_tropheryma_whipplei_NP_789261 257-286 LATLVVNKIRGIFKSVAVKAPGFGDRRKMMGroEL_rickettsia_bellii_YP_537760 259-288 LATLVVNRLRGGLKVAAVKAPGFGDRRKAMGroEL_anaplasma_phagocytophilium_HZ_YP_504857 258-287 LSTLVLNKLRGGLQVAAVKAPGFGDRRKDMGroEL_Burkholderia_multivorans_YP_001947677 259-288 LATLVVNAMRGILKVAAVKAPGFGDRRKAMGroEL_E_coli_K12_AAC77103 259-288LATLVVNTMRGIVKVAAVKAPGFGDRRKAMGroEL_CH60_CHLPN_chlamydia_pneumoniae 259-288 LATLVVNRLRAGFRVCAVKAPGFGDRRKAMGroEL_Staph_aureus_MRSA_YP_041479 257-286 LTNIVLNRMRGTFTAVAVKAPGFGDRRKAMGroEL_Mycobact_tuberc_CAA17397 257-286 LSTLVVNKIRGTFKSVAVKAPGFGDRRKAMGroEL_mycoplasma_penetrans_NP_757486 258-287 LTTLVVNKMRGVFNVVAVKAPEFGDKRKQVHSP60_Leishmania_Strain_Friedlin_XP_001685504 272-301 MHTFLYNKIQGRISGCAVKAPGFGDMRINQHSP60_Schistosoma_mansoni_XP_002572332 276-305 LTALVLNRLKLGLQVCAVKAPGFGDNRKNTHSP60_Plasmodium_falciparum_XP_001347438 288-317 LATLIVNKLRLGLKICAVKAPGFGEHRKALGroEL_Leptospira_interrogans_YP_001299 258-287 LATIVVNTLRKTISCVAVKAPGFGDRRKSMGroEL_Listeria_monocytogenes_AF335323_2?257-286 QATLVLNKLRGTFNVVAVKAPGFGDRRKAMGroEL_streptococcus_pneumoniae_AF325449_1?257-286 LPTLVLNKIRGTFNVVAVKAPGFGDRRKAMGroEL_treponema_pallidum_YP_001933036 258-287 LATLVVNSLRGTLKTCAVKAPGFGDRRKEMChaperonin60_Giardia_lamblia_XP_001705532 261-290 LSTLAINTLKGTVRCCAVRAPGYGDVKKGVchaperonin60_Cryptosporidium_parvum_XP_627821 291-320 LTALILNKLQLNLKVCAVKAPGFGDHRKQIChaperonin60_Entamoeba_histolytica_XP_656268 265-294 LTTLVLNKLRGLPIAAVRAPGFGETRKGILHResults with the above peptides are shown in figure 2 with the following abbreviations:The G20c homologs were 5. Escherichia coli (ESCHCOLI), 6. Legionella pneumophila (LEGIONELL), 7. Burkholderia multivorans (BURKHMULT), 8. Bartonella henselae (BARTHENS), 9. Borrelia garinii (BORRGAR), 10. Treponema pallidum (TREPPALL),11. Leptospira interrogans (LEPTINTERR), 12. Chlamydia pneumoniae (CHLAMPNEUM), 13. Mycobacterium tuberculosis (MYCTUB), 14. Tropheryma whipplei (TROPHWHIP), 15. Staphylococcus aureus (STAPAUR), 16. Listeria monocytogenes (LISTMONO), 17. Streptococcus pneumoniae (STREPTPNEU), 18. Mycoplasma penetrans (MYCPEN), 19. Neoehrlichia mikurensis (NEOMIK), 20. Anaplasma phagocytophilium (ANAPPHAG), 21. Wolbachia (WOLBAC), 22. Orientia tutsugamishi (ORITSU), 23. Rickettsia bellii (RICKBELL), 24. Leishmania major (LEISHMAN), 25. Giardia lamblia (GIARLAMB), 26. Entamoeba histolytica (ENTHIS), 27. Plasmodium falciparum (PLASFALC), 28. Cryptosporidium parvum (CRYPTPARV), 29. Schistosoma mansoni (SCHISMANS) (cf. Supplementary Table ST3).All G20c homologs gave high NTC values (100-900 MFI). The values presented in Figure 2 were partially subtracted with the NTC value as described in Materials and methods. The subtraction was uniform, because equal numbers of patient and control samples were always analyzed together. It could therefore not create artificial statistical differences between the groups. Rather, occasional oversubtraction where negative values were set to 0 could reduce, not exaggerate, differences and significances. G20c homolog peptides with the highest positive predictive (100%) and negative predictive values (62-68%) for ME in the IgM test were Staphylococcus at cutoff 66 MFI, Plasmodium at cutoff 321 MFI, Listeria at cutoff 198 MFI, Burkholderia at cutoff 278 MFI, Chlamydia at cutoff 244 MFI and Legionella at cutoff 271 MFI. Table ST4. The amino acid sequences of Mycoplasma penetrans GroEl peptides (GenBank id NP_757486).Peptide name Peptide sequence D1GroEL_Mycopl_pen 1-30 MAKEIKFSDSARNKLFNGVQQLFDAVKVTMD2GroEL_Mycopl_pen 16-45 FNGVQQLFDAVKVTMGPRGRNVLIQKSYGAD3GroEL_Mycopl_pen 31-60 GPRGRNVLIQKSYGAPVITKDGVSVAKEVDD4GroEL_Mycopl_pen 46-75 PVITKDGVSVAKEVDLTNPIENMGAQLVKDD5GroEL_Mycopl_pen 61-90 LTNPIENMGAQLVKDVASKTADEAGDGTTTD6GroEL_Mycopl_pen 76-105 VASKTADEAGDGTTTATVLAYGVFKEGLRND7GroEL_Mycopl_pen 91-120 ATVLAYGVFKEGLRNVISGANPIEIKRGMDD8GroEL_Mycopl_pen 106-135 VISGANPIEIKRGMDKTVNAIVNELNKSSKD9GroEL_Mycopl_pen 121-200 KTVNAIVNELNKSSKKIARKDEIIQVATISD10GroEL_Mycopl_pen 136-165 KIARKDEIIQVATISANSDKKIGELIANAMD11GroEL_Mycopl_pen 201-180ANSDKKIGELIANAMEKVGSDGVITVEEAKD12GroEL_Mycopl_pen 166-195 EKVGSDGVITVEEAKGINDELTVVEGMQFDD13GroEL_Mycopl_pen 181-210 GINDELTVVEGMQFDRGYISPYFVTDTNKMD14GroEL_Mycopl_pen 196-225 RGYISPYFVTDTNKMIAKLENPYILITDKKD15GroEL_Mycopl_pen 211-240 IAKLENPYILITDKKVSSIKDILPILEEIMD16GroEL_Mycopl_pen 226-255 VSSIKDILPILEEIMKTGRPLLIIADDVDGD17GroEL_Mycopl_pen 241-270 KTGRPLLIIADDVDGEALTTLVVNKMRGVFD18GroEL_Mycopl_pen 256-285 EALTTLVVNKMRGVFNVVAVKAPEFGDKRKD19GroEL_Mycopl_pen 271-300 NVVAVKAPEFGDKRKQVLEDIAILTGGSFVD20GroEL_Mycopl_pen 286-320 QVLEDIAILTGGSFVTDDLGISFDKVTLQDD21GroEL_Mycopl_pen 301-330 TDDLGISFDKVTLQDLGQAESVVIDKDNSTD22GroEL_Mycopl_pen 316-345 LGQAESVVIDKDNSTIVKGKGLESQIKERID23GroEL_Mycopl_pen 331-360 IVKGKGLESQIKERISKIKTAIEMTDSDYDD24GroEL_Mycopl_pen 346-375 SKIKTAIEMTDSDYDKDSLRNRLAKLNKGVD25GroEL_Mycopl_pen 361-390 KDSLRNRLAKLNKGVAVIKVGAVSEVELKED26GroEL_Mycopl_pen 376-405 AVIKVGAVSEVELKEKKDRVDDALSATKAAD27GroEL_Mycopl_pen 391-420 KKDRVDDALSATKAAIEEGIVIGGGAALVHD28GroEL_Mycopl_pen 406-435 IEEGIVIGGGAALVHVSKRINVNTLNLIGDD29GroEL_Mycopl_pen 421-450 VSKRINVNTLNLIGDEKIGYQIVMSAIMSPD30GroEL_Mycopl_pen 436-465 EKIGYQIVMSAIMSPISQIVSNAGFDKGVVD31GroEL_Mycopl_pen 451-480 ISQIVSNAGFDKGVVINEILKATNPHLGFND32GroEL_Mycopl_pen 466-495 INEILKATNPHLGFNAATGKYVDMFQTGIID33GroEL_Mycopl_pen 481-510 AATGKYVDMFQTGIIDPVKVTRIALQNAVSD34GroEL_Mycopl_pen 496-525 DPVKVTRIALQNAVSVSSMLLTTEAVIYDVD35GroEL_Mycopl_pen 511-540 VSSMLLTTEAVIYDVKDDKEDSVPAMPNMGD36GroEL_Mycopl_pen 526-555 KDDKEDSVPAMPNMGMGGMMTable ST5. The amino acid sequences of overlapping Chlamydia pneumoniae GroEL (GenBank id NP_224342) peptides. The peptides were synthesized with short (tri-ethylene glycol, C series) and long (hexa-ethylene glycol, E series) spacers. Yellow background: Peptides recognized by IgG in sera from women with Chlamydia trachomatis associated ectopic pregnancy, Yi et al PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5ZaTwvQXV0aG9yPjxZZWFyPjE5OTM8L1llYXI+PFJlY051

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ADDIN EN.CITE.DATA (8). Most Chlamydia pneumoniae peptides had an identical sequence. Positions which differed were underlined. The overlapping epitopes of peptides C30-C32 and E30-E32 defined by Yi et al were indicated by additional overstrike and italic marks.ShortSpacerLongSpacerPeptide namePeptide sequenceC1E1GroEL_Chlamyd_pneum 1-30MVAKNIKYNEEARKKIQKGVKTLAEAVKVTC2E2GroEL_Chlamyd_pneum 16-45IQKGVKTLAEAVKVTLGPKGRHVVIDKSFGC3E3GroEL_Chlamyd_pneum 31-60LGPKGRHVVIDKSFGSPQVTKDGVTVAKEVC4E4GroEL_Chlamyd_pneum 46-75SPQVTKDGVTVAKEVELADKHENMGAQMVKC5E5GroEL_Chlamyd_pneum 61-90ELADKHENMGAQMVKEVASKTADKAGDGTTC6E6GroEL_Chlamyd_pneum 76-105EVASKTADKAGDGTTTATVLAEAIYTEGLRC7E7GroEL_Chlamyd_pneum 91-120TATVLAEAIYTEGLRNVTAGANPMDLKRGIC8E8GroEL_Chlamyd_pneum 106-135NVTAGANPMDLKRGIDKAVKVVVDQIKKISC9E9GroEL_Chlamyd_pneum 121-200DKAVKVVVDQIKKISKPVQHHKEIAQVATIC10E10GroEL_Chlamyd_pneum 136-165KPVQHHKEIAQVATISANNDAEIGNLIAEAC11E11GroEL_Chlamyd_pneum 201-180SANNDAEIGNLIAEAMEKVGKNGSITVEEAC12E12GroEL_Chlamyd_pneum 166-195MEKVGKNGSITVEEAKGFETVLDVVEGMNFC13E13GroEL_Chlamyd_pneum 181-210KGFETVLDVVEGMNFNRGYLSSYFATNPETC14E14GroEL_Chlamyd_pneum 196-225NRGYLSSYFATNPETQECVLEDALVLIYDKC15E15GroEL_Chlamyd_pneum 211-240QECVLEDALVLIYDKKISGIKDFLPILQQVC16E16GroEL_Chlamyd_pneum 226-255KISGIKDFLPILQQVAESGRPLLIIAEDIEC17E17GroEL_Chlamyd_pneum 241-270AESGRPLLIIAEDIEGEALATLVVNRIRGGC18E18GroEL_Chlamyd_pneum 256-285GEALATLVVNRIRGGFRVCAVKAPGFGDRRC19E19GroEL_Chlamyd_pneum 271-300FRVCAVKAPGFGDRRKAMLEDIAILTGGQLC20E20GroEL_Chlamyd_pneum 286-320KAMLEDIAILTGGQLISEELGMKLENANLAC21E21GroEL_Chlamyd_pneum 301-330ISEELGMKLENANLAMLGKAKKVIVSKEDTC22E22GroEL_Chlamyd_pneum 316-345MLGKAKKVIVSKEDTTIVEGMGEKEALEARC23E23GroEL_Chlamyd_pneum 331-360TIVEGMGEKEALEARCESIKKQIEDSSSDYC24E24GroEL_Chlamyd_pneum 346-375CESIKKQIEDSSSDYDKEKLQERLAKLSGGC25E25GroEL_Chlamyd_pneum 361-390DKEKLQERLAKLSGGVAVIRVGAATEIEMKC26E26GroEL_Chlamyd_pneum 376-405VAVIRVGAATEIEMKEKKDRVDDAQHATIAC27E27GroEL_Chlamyd_pneum 391-420EKKDRVDDAQHATIAAVEEGILPGGGTALIC28E28GroEL_Chlamyd_pneum 406-435AVEEGILPGGGTALIRCIPTLEAFLPMLTNC29E29GroEL_Chlamyd_pneum 421-450RCIPTLEAFLPMLTNEDEQIGARIVLKALSC30E30GroEL_Chlamyd_pneum 436-465EDEQIGARIVLKALSAPLKQIAANAGKEGAC31E31GroEL_Chlamyd_pneum 451-480APLKQIAANAGKEGAIIFQQVMSRSANEGYC32E32GroEL_Chlamyd_pneum 466-495IIFQQVMSRSANEGYDALRDAYTDMLEAGIC33E33GroEL_Chlamyd_pneum 481-510DALRDAYTDMLEAGILDPAKVTRSALESAAC34E34GroEL_Chlamyd_pneum 496-525LDPAKVTRSALESAASVAGLLLTTEALIAEC35(m)E35g(m)GroEL_Chlamyd_pneum 511-540SVAGLLLTTEALIAEIPEEKPAAAPAMPGAC36E36GroEL_Chlamyd_pneum 526-555IPEEKPAAAPAMPGAGMDYPolyethylene glycol of varying length has earlier been used as an inert spacer to display peptides with minimal spatial constraint on surfaces PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5YdWU8L0F1dGhvcj48WWVhcj4yMDExPC9ZZWFyPjxSZWNO

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ADDIN EN.CITE.DATA (9). The spacer can restrict the availability of a peptide to antibodies. Potentially it could lead to false negative results in SMIA, and we wanted to investigate this variable. It turned out that an IgG epitope that was not visible in the scan with Chlamydia peptides with a short spacer was prominent in the scan with a longer spacer (E35). On the other hand, an epitope which was relatively strongly recognized with a short spacer (C25) was weaker with a long spacer. All peptides were anchored at the amino terminal end. It is possible that further epitopes could have been discovered if peptides anchored at the carboxy terminus also were tested.Supplementary table ST6. Sequences of ”strongly binding peptides” (SBPs), taken from Chen and Sigler ADDIN EN.CITE <EndNote><Cite><Author>Chen</Author><Year>1999</Year><RecNum>5784</RecNum><DisplayText>(<style face="italic">1</style>)</DisplayText><record><rec-number>5784</rec-number><foreign-keys><key app="EN" db-id="f29rxsde60p9fsev5d9v9relserrtavztsvz">5784</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Chen, L.</author><author>Sigler, P. B.</author></authors></contributors><auth-address>Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511, USA.</auth-address><titles><title>The crystal structure of a GroEL/peptide complex: plasticity as a basis for substrate diversity</title><secondary-title>Cell</secondary-title><alt-title>Cell</alt-title></titles><periodical><full-title>Cell</full-title></periodical><alt-periodical><full-title>Cell</full-title></alt-periodical><pages>757-68</pages><volume>99</volume><number>7</number><edition>2000/01/05</edition><keywords><keyword>Chaperonin 10/*chemistry</keyword><keyword>Chaperonin 60/*chemistry</keyword><keyword>Escherichia coli/*chemistry</keyword><keyword>Fluorescence Polarization</keyword><keyword>Magnetic Resonance Spectroscopy</keyword><keyword>Models, Molecular</keyword><keyword>Molecular Sequence Data</keyword><keyword>Protein Binding</keyword><keyword>Protein Conformation</keyword><keyword>Protein Structure, Tertiary</keyword><keyword>X-Ray Diffraction</keyword></keywords><dates><year>1999</year><pub-dates><date>Dec 23</date></pub-dates></dates><isbn>0092-8674 (Print)&#xD;0092-8674 (Linking)</isbn><accession-num>10619429</accession-num><work-type>Research Support, Non-U.S. Gov&apos;t&#xD;Research Support, U.S. Gov&apos;t, P.H.S.</work-type><urls><related-urls><url>;(1). In that publication, the peptides were selected by panning with E. coli HSP60 from a peptide library. The biotinylated hexaethylene glycol spacer is written as Bio-(EG)6.Peptide namePeptide sequenceSBP1Bio-(EG)6- SWMTTPWGFLHPSBP2Bio-(EG)6- FHYEIWIPPHRGSBP3Bio-(EG)6- SSPWWLVSFTSTSBP4Bio-(EG)6- SHSLIWRIPLLHSBP5Bio-(EG)6- IYVPWYYAENLPSection 3. Details regarding the SMIA procedure Coupling of proteins and peptides to carboxylated microspheres The synthetic peptides or protein were coupled to Luminex Carboxylated Microspheres, as described in the “Sample protocol for two step carbodiimide coupling of protein to carboxylated microspheres” provided by the Luminex Corporation (Austin, TX). Briefly, the stock uncoupled beads (xMAP Technology, Austin, TX) were vortexed for 20 s, then sonicated for 20 s. An appropriate amount (i.e. 100 - 400 ?l) of the stock microspheres, containing 1.25 × 107?beads per ml, was transferred to a 1.5 ml Eppendorf tube which then was centrifuged at 13,000 ×?g?for 3 min. The supernatant was carefully removed and the beads were resuspended in 100 μl of distilled water, followed by vortexing and sonication for 20 s, and then centrifuged at 13,000 ×g?for 3 min. Supernatants were subsequently removed and resuspended in 80 ?l of 100 mM monobasic sodium phosphate (MSP, Sigma, cat. nr?S3139) pH 6.2 , then vortexed and sonicated for 20 s. Ten microliters of freshly made?N-hydroxysuccinimide (NHS)(Pierce, cat. nr 24510) and 10 μl of 50-mg/ml 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC; water-soluble carbodiimide; Pierce, cat. nr 25952-53-8 ) in H2O were added to the beads. The suspension was then incubated in the dark for 20 min at room temperature. The beads were then centrifuged as described above. The supernatant was carefully removed, and the pellet was washed twice in 250 μl of 50 mM 2-(N-morpholino) ethanesulfonic acid (MES) sodium salt (Sigma, cat. nr 71119-23-8), pH 5. The supernatant was removed and the beads resuspended in an appropriate volume of the MES buffer (i.e. 125 ?l MES to 100?l of beads), vortexed and sonicated. Fifty micrograms of the peptide or 1- 100 ?g protein was added. Beads were mixed by careful vortexing and then incubated in the dark for 2 hours at room temperature on a plate shaker (100 rpm). The coupled microspheres were pelleted by centrifugation at 13,000 ×?g?for 3 min and resuspended in 500 μl of StabilGuard buffer (SurModics, Eden Prairie, MN, cat. nr SG01-1000 ). The coupled microspheres were again pelleted by centrifugation as described above and resuspended in 1 ml of StabilGuard buffer twice. The final pellet was resuspended in an appropriate amount of StabilGuard buffer (1000 ?l of StabilGuard buffer if 100?l of beads were taken). This created a bead mixture consisting of 1250 beads/μl. The coupled beads were stored at 4°C in the dark.Ten ?g of Haemophilus influenzae?type b (Hib) vaccine (Act-HIB?; Sanofi Pasteur MSD) was diluted in 100?L sterile water and coupled to the Luminex bead following the coupling protocol. An unprocessed (“naked”) bead, without any bound antigen, was used to control for nonspecific binding to the Luminex beads. The stock uncoupled beads were vortexed for 20 s, then sonicated for 20 s. An appropriate amount of the stock microspheres was transferred to a 1.5 ml Eppendorf tube which then was centrifuged at 13,000 ×?g?for 3 min. The supernatant was carefully removed and the beads were resuspended in 100 μl of distilled water, followed by vortexing and sonication for 20 s, and then centrifuged at 13,000 ×g?for 3 min. Supernatants were subsequently removed and resuspended in 500 ?l of StabilGuard. The uncoupled beads were again pelleted by centrifugation as described above and resuspended in 1 ml of StabilGuard buffer twice. The final pellet was resuspended in StabilGuard buffer to 1250 beads/μl. The coupled beads in this stock suspension were stored at 4°C in the dark.Preanalytical proceduresSamples were immediately frozen in small aliquots, to avoid repetitive freezing and thawing.Suspension Multiplex Immunoassay (SMIA)During the development of SMIA for many of the pathogens previously associated with ME we noted that the sensitivity of the SMIA was the same as, or better than, EIA, with a wide dynamic range and possibility to simultaneously read 100 analytes in the Luminex? 200? analyzer (Luminex Corporation, Austin, Texas) (J Blomberg, unpublished, and PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5CbG9tYmVyZzwvQXV0aG9yPjxZZWFyPjIwMTI8L1llYXI+

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ZE5vdGU+

ADDIN EN.CITE.DATA (10, 11). The assay system required only 5-10 ?l of serum or plasma. Antigen (10-50 ?g of whole microbe lysate, recombinant protein and synthetic peptide) was coupled covalently to carboxylated color-coded beads as described above. IgG was detected using biotinylated protein G (BPG) PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5Ba2Vyc3Ryb208L0F1dGhvcj48WWVhcj4xOTg1PC9ZZWFy

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ADDIN EN.CITE.DATA (12, 13) as secondary antibody. For IgM antibody detection biotinylated anti-human IgM (affinity purified, μ-chain specific, Sigma-Aldrich cat. Nr. B1140) was used as secondary antibody. A few control experiments with 4 ?g/ml biotinylated monoclonal anti-IgG (BioLegend inc, San Diego, CA, cat. nr. 409307) and 4 ?g/ml biotinylated monoclonal anti-IgM (BioLegend, cat nr. 314504) are reported in the Supplementary Figure SF3. Preparations prior to filter plate loadingSamples were diluted prior to loading the filter plate using StabilGuard as a diluent. Each ME patient plasma sample was diluted 1:10 (this dilution included the dilution during plasma preparation). BD serum samples were also diluted 1:10. After a concluded coupling the bead stock solution contained 1250 beads/ ?l. For a typical experiment, a bead mixture consisting of 25 beads/μl was made using StabilGuard as a diluent. All the beads with coupled proteins or peptides as well as the naked bead and Hemophilus influenzae B bead were sonicated and vortexed for 20 s before being added to the bead mixture. A fetuin bead (see below) was included in IgM experiments as an additional positive control.Filter plate loading and washingFor an IgG run, the standard Luminex protocol for the filter plate, namely “Sample protocol for indirect antibody capture immunoassay” was largely followed PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5TaGVpa2hvbHZhZXppbjwvQXV0aG9yPjxZZWFyPjIwMTE8

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ADDIN EN.CITE.DATA (10, 11), with the exception that coupling conditions were different. Briefly, the multiplex assay was carried out in a 96 well Multiscreen? filter plates (Millipore, UK, cat. nr MSHVN4B10) with 0.45?μm pore size. Initially, the filter plate was pre-wetted twice with 100 ?l PBS (pH7.4).The PBS was removed from the wells by aspiration through the MultiScreenHTS?vacuum manifold?(Millipore, USA, cat. nr. MSVMHTS0D). Fifty microliters of serum diluted 1:10 in StabilGuard buffer were added to each well, except one, a well containing only StabilGuard and beads, as a non-template control (NTC). The bead mixture was sonicated and vortexed for 20 s. Fifty microliters of the bead mix was then added to each well. After this, the wells were incubated in the dark for 30 min at room temperature on a plate shaker. After incubation, the plate was aspirated and washed with 100 ?l PBS twice. Fifty ?l StabilGuard were added and followed by 50?l of a 4?g/ml BPG solution (Pierce, cat. nr 29988) diluted in StabilGuard. The beads were resuspended by pipetting up and down five times. The plate was incubated in the dark for 30 min at room temperature on a plate shaker (100 rpm). After incubation, the wells were aspirated and washed twice with 100 ?l PBS. 50?l StabilGuard were added to each well and followed by 50 ?l of a 4 μg/ml streptavidin-phycoerythrin (SA-PE) solution (Life Technologies Europe, cat. nr SA1004-4) diluted in StabilGuard, and the beads were resuspended by pipetting up and down five times. The plate was then incubated in the dark for 15 min at room temperature on a plate shaker. After incubation, the wells were aspirated and washed twice with 100 μl PBS. The filter plate well was resuspended in 150 μl of PBS. One hundred μl were then analyzed on the Luminex? 200? system analyzer following the manufacturer's instructions. The StarStation (Applied Cytometry, Sheffield, UK) or xPONENT? (Luminex corp. Austin, Tx) softwares were used to analyze the data.For an IgM run, filter plate loading was done as described for IgG, with the exception that 0.4 ?g/ml of anti-human IgM (Biotin conjugated, μ-chain specific, Sigma-Aldrich, cat. nr. B1265) was used as a secondary antibody instead of Protein G. The HIB vaccine bead, intended as a positive control for IgG detection, proved to work also as a positive control for IgM detection. The HIB vaccine is a polysaccharide conjugated to tetanus toxoid, and it is not surprising that it also detects IgM, because anti-carbohydrate antibodies tend to be of IgM type. As a further positive control for presence of IgM (Katona and Blomberg, unpublished), we used a bead coupled with 100 μg of bovine fetuin (Sigma-Aldrich, cat. nr F2379), also called a2HS-Glycoprotein. Fetuin is a heavily glycosylated glycoprotein which reacts with anti-betagalactoside antibodies. The majority of humans have such IgM antibodies.Specificity of the MFI signalsSupplementary figure SF1.NTC Protein G vs NTC monoclonal anti IgG for 24 human HSP60 peptides (G1-G24).Correlation between protein G and monoclonal anti-IgG values for a HIB-coupled bead, and 24 ME samples.Correlation between protein G and monoclonal anti-IgG for a G20c-coupled bead, and 22 ME samples. Correlation between poly- and monoclonal anti-IgM for a HIB-coupled bead and 39 ME samples.In experiments using Strongly Binding Peptides, SBPs, the five biotinylated SBPs (SBP1-SBP5, see Supplementary Table ST6) were dissolved in sterile phosphate-buffered saline (PBS), pH 7 and 10 to 50% DMSO. For a Strongly Binding Peptide assay, filter plate loading was done as described for IgG, with the exception that 500, 50, 5 and 0.5 ?g/ml of each five biotinylated SBP (SBP1-SBP5) was used instead of Protein G. Fifty microliters of sample (IgG diluted 1:10, ME pool diluted 1:5, and ME pool diluted 1:10) diluted in StabilGuard buffer was added to each well. One well containing only StabilGuard was used as a non-template control “NTC” well. Fifty microliters of the bead mix (containing beads with human HSP60 G20, G20a, G20b, G20c, G20d, G20e, G20f, recombinant human HSP60, GroEL recombinant protein, Hemophilus influenzae B, and naked beads) was then added to each well. Four different concentrations for each of the five biotinylated SBPs (500, 50, 5 and 0.5 ?g/ml) were incubated for 30 minutes. The beads were then washed, and incubated with SA-PE like in the experiments with biotinylated protein G and anti human IgM, and read in the Luminex bead counter. The results are described in Supplementary Figure SF2. Supplementary figure SF2. Binding of “strongly binding peptides” (SBPs) to HSP60 recombinant proteins and human HSP60 G20 and G20c peptides. Out of the five SBPs, SBP2 and SBP4, but not the other SBPs, bound to recombinant human HSP60, E coli GroEL, G20c and G20c homolog peptides, suggesting a chaperonin-like peptide binding activity of G20c homolog peptides. Signals of ME sera with high MFI values to these antigens were not much diminished. B and C. SBP2 and SBP4 bound without saturation effects, with a concentration of 5 ?g/ml being optimal for further experiments.Absorption of IgG with protein A.A source of falsely positive IgM values is IgG anti-IgM (“rheuma factor”), which occurs in autoimmune diseases like rheumatoid arthritis. Ten strongly IgM positive and 6 weakly IgM positive ME samples and 6 blood donor sera were incubated with recombinant Protein A Sepharose? Fast Flow and Sepharose? Fast Flow beads alone (17-1279-01, 17-0149-01, GE Healthcare, Bio-Sciences AB Uppsala, Sweden) at 37°C on a shaker for 1 h. The samples were centrifugated for 3 minutes at 13000 rpm. The supernatant was subsequently tested in the IgG and IgM SMIA tests, as described in Methods. In the IgG test, 0-30% of the MFI were recovered, while 60-150% of the MFI were recovered in the IgM test (data not shown). The result makes it unlikely that rheuma factor was a major cause of the IgM values in the ME petition experiments using blocking protein and SBPs.During the course of this investigation we found that including ovalbumin together with the secondary antibody drastically reduced the NTC values with a marginal effect on the antibody specific signals from the samples (Supplementary Figure SF2). Obviously, the human blood samples contained enough non-antibody protein (a typical serum or plasma contains 70 mg/ml) to block the low affinity detected by the NTC. The StabilGuard diluent does not contain protein. We did not routinely include highly concentrated blocking protein in the tests because of occasional clogging of the probe in the Luminex machine in the presence of blocking protein. Instead, we opted for a partial subtraction of NTC values, as described in Materials and Methods (Supplementary Figure SF3).A few peptides, mostly those containing the helix I of human and microbial HSP60s gave high NTC values, making this control less useful. The binding in the presence of patient sample could be lower than that of the NTC (binding of the signal generating system to the bead without presence of patient sample). We therefore tried to reduce the NTC values by addition of 0.1, 1, 10 and 100 ?g/ml Ovalbumin (Albumin from chicken egg white), (Sigma-Aldrich, cat. nr A 5503) and Bovine Serum Albumin (Bovine Plasma Albumin; BSA), (Sigma-Aldrich, cat. nr A3913) during incubation with BPG or SA-PE. A tenfold reduction of the NTC value of bead-bound G20c, without affecting the MFI of wells containing patient samples, was obtained by inclusion of 100 ?g/mL of ovalbumin during the incubation with BPG while BSA was less effective. However, the ovalbumin was not routinely added because of occasional clogging of the Luminex machine probe. NTC MFI were therefore partially subtracted from the MFI of patient and BD samples as described petition experiments with blocking proteins were conducted using 0.1, 1, 10 and 100 ?g/ml ovalbumin and the same concentrations of BSA (not shown) during incubation with BPG or SA-PE during the incubations with protein G and SA-PE. The results are described in Supplementary Figure SF3.Supplementary Figure SF3. Competition experiments with high concentrations of blocking protein. Ovalbumin (and BSA, not shown) was added at the protein G and SAPE incubation steps. The IgG preparation (5 mg/ml), the ME pool and ME patient and a BD sample with high IgG binding to the three antigens were run without and with different concentrations of ovalbumin at the protein G/anti-IgM incubation stage. The effect of ovalbumin was stronger than that of BSA at the same weight percentages. Results for peptides G20 (A), G20c (B) and E. coli GroEL (HSP60) (C) are shown. Data management and StatisticsA computer program (MultiPlus?) which subtracts the NTC and naked bead values, and checks the results of the control sera for each peptide and protein, and stores them in a relational database, was written in Visual Foxpro by J. Blomberg. The subtraction was trivial for nearly all naked bead values, which were 5-10 MFI, but nontrivial for a few peptides, where the NTC values could reach several hundred MFI. In these cases the lowest MFI obtained with some samples were often lower than the NTC value. To avoid oversubtraction, the subtraction was made with 10% of the NTC and 90% of the lowest sample value for the bead. Negative values after subtraction were set to 0 MFI. The experiments always contained an equal number of patient and control samples, so the two groups were always treated equally. The validity of the subtraction was ascertained in later experiments, when we found that the NTC values could be reduced by inclusion of ovalbumin during the incubation with BPG or biotinylated anti-IgM. Subtracted results with and without the ovalbumin inclusion were highly similar (Supplementary Figure SF1). Significant differences in antigen reactivity between ME patient and BD samples were automatically evaluated using the two-tailed Fisher exact test (FET) and two-tailed Wilcoxon rank sum tests (WRST) with computer procedures written by J. Blomberg. A cutoff of 200 MFI was used in the FET unless stated otherwise. To reduce the influence of weak binding values in the WRST, all MFI values were subtracted with 50, with resulting negative values listed as 0, before WRST analysis. At this initial stage, no (“Bonferroni”) correction of p values for repeated comparisons was made. Cross-correlation of variables to each other, and principal component analysis (PCA), was performed using the Unscrambler statistical package (version X, Camo AS, Bergen, Norway).Section 4. Further results from the phyloscanning with G20c homologsSupplementary Figure SF5. Needle plots of reactivities with Test set samples with G20c homolog peptides in A. IgG and B. IgM assays. MFI values subtracted with NTC MFI of selected G20c homologs from the phyloscanning. Results from G20c homologs of a protozoon and bacteria are presented separately. Although the ME preference in most IgG tests was inferior to that of the IgM tests it is shown for comparison. The patient group was here split into ME (lane 1), FM (lane 2), ME+FM (lane 3), ME+IBS (lane 4), ME+FM+IBS (lane 5) and FM+IBS (lane 6) groups. BD samples were in lane 7 and control samples in lane 0. The most strongly discriminating peptides are framed in red. A cutoff which may be useful if the peptide is to be used as a biomarker,based on the Test set alone, is also shown in red. For abbreviations, see the legend of Figure 5. Supplementary table ST7. Correlation coefficients of MFI of G20c homolog peptides and recombinant human and E. coli HSP60 proteins in a serological assay with patient and BD samples. Correlation coefficients and sequence similarity according to the BLOSUM62 scoring system (“blosum”) are included.Table ST7A. The 20 highest correlations between the IgG binding (MFI) of G20c homologs.Five of the combinations were discordant, between a prokaryotic and a eukaryotic HSP60 sequence (*). Sequence 1Sequence 2DiscordantCombinationblosumIggcorrIgmcorrEscherichia coliBurkholderia multivorans1350,992280,64098Treponema pallidumMycobacterium tuberculosis1160,969780,39078Leptospira interrogansLegionella pneumophila990,961670,78119Burkholderia multivoransBartonella henselae1260,938480,55282Escherichia coliBartonella henselae1240,938090,27021Escherichia coliLegionella pneumophila1350,936690,5946Leptospira interrogansChlamydia pneumoniae1020,932660,84728Anaplasma phagocytophiliumWolbachia1320,930760,42341Staphylococcus aureusHomo sapiens*900,927630,46618Schistosoma mansoniiHomo sapiens1270,913050,37872Rickettsia belliiCandidatus neoehrlichia mikurensis1240,910030,51955Leptospira interrogansEscherichia coli1040,901080,66396Chlamydia pneumoniaeHomo sapiens*1000,899570,52151Leptospira interrogansBurkholderia multivorans1000,896390,87349Leptospira interrogansPlasmodium falciparum*920,895070,46447Leptospira interrogansHomo sapiens*890,895010,45683Anaplasma phagocytophiliumBartonella henselae1300,8870,69313Leptospira interrogansStaphylococcus aureus1000,8820,81089Leptospira interrogansSchistosoma mansonii*770,879070,66144Borrelia_gariniiHomo sapiens*830,869630,38378 Table ST7B. The 20 highest correlations between the IgM binding (MFI) of G20c homologs. One of the combinations was discordant, between a prokaryotic and a eukaryotic HSP60 sequence (*). Sequence 1Sequence 2DiscordantcombinationblosumIggcorrIgmcorrBorrelia gariniiLegionella pneumophila1230,866840,93864Rickettsia belliiBartonella henselae1410,722580,87917Leptospira interrogansBurkholderia multivorans1000,896390,87349Rickettsia belliiTropheryma whipplei1090,79940,86233Chlamydia pneumoniaeBurkholderia multivorans1100,711030,85463Leptospira interrogansChlamydia pneumoniae1020,932660,84728Treponema pallidumLegionella pneumophila1240,7410,84472Leptospira interrogansStaphylococcus aureus1000,8820,81089Leptospira interrogansLegionella pneumophila990,961670,78119Mycobacterium tuberculosisTropheryma whipplei1310,819510,77923Chlamydia pneumoniaeLegionella pneumophila1190,854730,76039Treponema pallidumStaphylococcus aureus970,437710,75873Treponema pallidumLeptospira interrogans1080,637750,74766Treponema pallidumBurkholderia_multivorans1160,677140,74684LeishmaniaHomo sapiens760,167060,74027Chlamydia pneumoniaeEscherichia coli1090,720240,73912Treponema pallidumSchistosoma mansonii*880,462950,71362Anaplasma phagocytophiliumTropheryma whipplei1020,810870,71279Leptospira_interrogansMycobacterium_tuberculosis1060,717610,70157Section 5. Alignment of HSP60 sequences.Supplementary Figure SF4. An alignment of 34 HSP60 sequences. Turqoise background: Epitopes crossreactive with HSP60 peptides of Mycobacterial, Chlamydial, E. coli and human origin, defined by Perschinka et al PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5QZXJzY2hpbmthPC9BdXRob3I+PFllYXI+MjAwMzwvWWVh

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ADDIN EN.CITE.DATA (2). Gray background: Porphyromonas gingivalis peptide related to autoimmunity Jeong et al ADDIN EN.CITE <EndNote><Cite><Author>Jeong</Author><Year>2012</Year><RecNum>3960</RecNum><DisplayText>(<style face="italic">3</style>)</DisplayText><record><rec-number>3960</rec-number><foreign-keys><key app="EN" db-id="f29rxsde60p9fsev5d9v9relserrtavztsvz">3960</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Jeong, E.</author><author>Lee, J. Y.</author><author>Kim, S. J.</author><author>Choi, J.</author></authors></contributors><auth-address>Department of Molecular Biology, College of Natural Sciences, Pusan National University, Pusan, South Korea Department of Periodontology, School of Dentistry, Pusan National University, Yangsan City, South Korea.</auth-address><titles><title>Predominant immunoreactivity of Porphyromonas gingivalis heat shock protein in autoimmune diseases</title><secondary-title>Journal of periodontal research</secondary-title><alt-title>J Periodontal Res</alt-title></titles><periodical><full-title>Journal of periodontal research</full-title><abbr-1>J Periodontal Res</abbr-1></periodical><alt-periodical><full-title>Journal of periodontal research</full-title><abbr-1>J Periodontal Res</abbr-1></alt-periodical><pages>811-6</pages><volume>47</volume><number>6</number><edition>2012/07/21</edition><dates><year>2012</year><pub-dates><date>Dec</date></pub-dates></dates><isbn>1600-0765 (Electronic)&#xD;0022-3484 (Linking)</isbn><accession-num>22812430</accession-num><urls><related-urls><url>;(3). Underlined are the i. nonapeptide from the leader of human HSP60 which binds HLA-E and promotes NK cell killing, Michaelsson et al PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5NaWNoYWVsc3NvbjwvQXV0aG9yPjxZZWFyPjIwMDI8L1ll

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ADDIN EN.CITE.DATA (4). Red text indicates homologs of human HSP60 G20c. Serologically reactive octameric peptides of Orientia tsutsugamushi Sta58, an HSP60 protein, (Lachumanan et al, ADDIN EN.CITE <EndNote><Cite><Author>Lachumanan</Author><Year>1993</Year><RecNum>5769</RecNum><DisplayText>(<style face="italic">15</style>)</DisplayText><record><rec-number>5769</rec-number><foreign-keys><key app="EN" db-id="f29rxsde60p9fsev5d9v9relserrtavztsvz">5769</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Lachumanan, R.</author><author>Devi, S.</author><author>Cheong, Y. M.</author><author>Rodda, S. J.</author><author>Pang, T.</author></authors></contributors><auth-address>Institute of Advanced Studies, University of Malaya, Kuala Lumpur.</auth-address><titles><title>Epitope mapping of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi</title><secondary-title>Infection and immunity</secondary-title><alt-title>Infect Immun</alt-title></titles><periodical><full-title>Infection and immunity</full-title><abbr-1>Infect Immun</abbr-1></periodical><alt-periodical><full-title>Infection and immunity</full-title><abbr-1>Infect Immun</abbr-1></alt-periodical><pages>4527-31</pages><volume>61</volume><number>10</number><edition>1993/10/01</edition><keywords><keyword>Amino Acid Sequence</keyword><keyword>Antibodies, Bacterial/immunology</keyword><keyword>Antigens, Bacterial/chemistry/immunology</keyword><keyword>Bacterial Outer Membrane Proteins/*immunology</keyword><keyword>Bacterial Proteins/*immunology</keyword><keyword>Epitopes</keyword><keyword>Humans</keyword><keyword>Molecular Sequence Data</keyword><keyword>Orientia tsutsugamushi/*immunology</keyword><keyword>Peptides/immunology</keyword><keyword>Scrub Typhus/*immunology</keyword></keywords><dates><year>1993</year><pub-dates><date>Oct</date></pub-dates></dates><isbn>0019-9567 (Print)&#xD;0019-9567 (Linking)</isbn><accession-num>7691753</accession-num><work-type>In Vitro&#xD;Research Support, Non-U.S. Gov&apos;t</work-type><urls><related-urls><url>;(15) are shown. Further details on HSP60 epitopes are given in section 6.The positions of the apical loops and helices, as defined in E. coli GroEL, Braig et al ADDIN EN.CITE <EndNote><Cite><Author>Braig</Author><Year>1994</Year><RecNum>3935</RecNum><DisplayText>(<style face="italic">16</style>)</DisplayText><record><rec-number>3935</rec-number><foreign-keys><key app="EN" db-id="f29rxsde60p9fsev5d9v9relserrtavztsvz">3935</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Braig, K.</author><author>Otwinowski, Z.</author><author>Hegde, R.</author><author>Boisvert, D. C.</author><author>Joachimiak, A.</author><author>Horwich, A. L.</author><author>Sigler, P. B.</author></authors></contributors><auth-address>Department of Genetics, Yale University School of Medicine, Boyer Center, New Haven, Connecticut 06510.</auth-address><titles><title>The crystal structure of the bacterial chaperonin GroEL at 2.8 A</title><secondary-title>Nature</secondary-title><alt-title>Nature</alt-title></titles><periodical><full-title>Nature</full-title><abbr-1>Nature</abbr-1></periodical><alt-periodical><full-title>Nature</full-title><abbr-1>Nature</abbr-1></alt-periodical><pages>578-86</pages><volume>371</volume><number>6498</number><edition>1994/10/13</edition><keywords><keyword>Amino Acid Sequence</keyword><keyword>Chaperonin 60/*chemistry</keyword><keyword>Computer Graphics</keyword><keyword>Crystallography, X-Ray</keyword><keyword>Escherichia coli</keyword><keyword>Models, Molecular</keyword><keyword>Molecular Sequence Data</keyword><keyword>Protein Conformation</keyword></keywords><dates><year>1994</year><pub-dates><date>Oct 13</date></pub-dates></dates><isbn>0028-0836 (Print)&#xD;0028-0836 (Linking)</isbn><accession-num>7935790</accession-num><work-type>Research Support, U.S. Gov&apos;t, P.H.S.</work-type><urls><related-urls><url>;(16) are also indicated. Alignment positions, referring to the numbers in Figure 7, are shown to the right. CLUSTAL W (1.83) multiple sequence alignment 50GroEL_orientia_tsutsugamushi_C --------------------------------MSKQIVHGDQCRKKIIEGGroEL_rickettsia_bellii_YP_537 -------------------------------MATKLIKHGSKAREQMLEGGroEL5_neoehrlichia_mikurensis --------------------------------MANVVVTGETLDKSIRDIGroEL_anaplasma_phagocytophili --------------------------------MSNTVVTGEVLDKSIREVGroEL_ehrlichia_deer_AB454077 --------------------------------MANVVVTGEQLDKSIREVGroEL_Wolbachia_YP_198181 --------------------------------MTNVVVSGEQLQEAFREVGroEL_Borrelia_garinii_YP_0730 --------------------------------MAKDIYFNEDARKSLLSGGroEL_treponema_pallidum_YP_00 --------------------------------MAKQLLFNEEARKKLLSGGroEL_Leptospira_interrogans_Y --------------------------------MAKDIEYNETARRKLLEGGroEL_CH60_CHLPN_chlamydophila -------------------------------MAAKNIKYNEEARKKIHKGGroEL3_chlamydia_psittaci_AEG8 -------------------------------MAAKNIKYNEDARKKIHKGGroEL2_salmonella_enterica_typ -------------------------------MAAKDVKFGNDARVKMLRGGroEL_salmonella_typhi_TY2_U01 -------------------------------MAAKDVKFGNDARVKMLRGGroEL_E_coli_K12_AAC77103 -------------------------------MAAKDVKFGNDARVKMLRGGroEL_Yersinia_pestis_NP_99189 -------------------------------MAAKDVKFGNDARIKMLRGGroEL_haemophilus_influenzae_Y -------------------------------MAAKDVKFGNDARVKMLKGGroEL_Legionella_YP_126086 --------------------------------MAKELRFGDDARLQMLAGGroEL_Burkholderia_multivorans -------------------------------MAAKDVKFHDGARSRIVKGGroEL_Bartonella_henselae_YP_0 -------------------------------MAAKEVKFGREARERLLRGGroEL_brucella_abortus_ZP_0587 -------------------------------MAAKDVKFGRTAREKMLRGGroEL_Mycobact_tuberc_CAA17397 --------------------------------MAKTIAYDEEARRGLERGGroEL_Mycobacterium_leprae_TN_ --------------------------------MAKTIAYDEEARRGLERGGroEL_tropheryma_whipplei_NP_7 --------------------------------MAKKITFNEDARRGLERGGroEL_Staph_aureus_MRSA_YP_041 --------------------------------MVKQLKFSEDARQAMLRGGroEL_Listeria_monocytogenes_A --------------------------------MAKDIKFSEDARRAMLRGGroEL_streptococcus_pneumoniae --------------------------------MSKEIKFSSDARSAMVRGGroEL_mycoplasma_penetrans_NP_ --------------------------------MAKEIKFSDSARNKLFNGHSP60_Leishmania_Strain_Friedl ------------------MLSRTVPRCVKYGSTPKDIRYGMEARNALLAGHSP60_Schistosoma_mansoni_XP_0 -------MLRAFATLRGT--LAPVRHRVIQRSYAKEVKFGADARSAMLVGHsp60_human_mitochondria_NP_95 -------MLRLPTVFRQMRPVSRVLAPHLTRAYAKDVKFGADARALMLQGHSP60_Plasmodium_falciparum_XP MISTLRGKIFN--NGSNRNKCVSILSNIQKRNISKDIRFGSDARTAMLTGchaperonin60_Cryptosporidium_p MLLRSGINLYKSVEGSIGLRSAAIRFGMRYISSGKELSFGGKARKEMLKGChaperonin60_Entamoeba_histoly ------------------------MLSSSSHYNGKLLSLNIDCRENVLSGChaperonin60_Giardia_lamblia_X -----------------------------MLQHYTSVISGEDARSGLLRG . . . 100GroEL_orientia_tsutsugamushi_C INVVANAVGITLGPKGRCVAIEQSYG--PPKITKDGVSVAKAIQLKDKSLGroEL_rickettsia_bellii_YP_537 IDILADAVKVTLGPKGRNVLIEQSFG--APKITKDGVTVAKSIELKDKIRGroEL5_neoehrlichia_mikurensis IRILEDAVGCTAGPKGLTIAISKPYG--TPEITKDGYKVIKSIKPEEPLAGroEL_anaplasma_phagocytophili VRILEDAVGCTAGPKGLTVAISKPYG--SPEITKDGYKVMKSIKPEEPLAGroEL_ehrlichia_deer_AB454077 VRILEDAVGCTAGPKGLTVAIGKSYG--APEITKDGYKVIKSIKPEDPLAGroEL_Wolbachia_YP_198181 AVMVDSTVAITAGPRGKTVGINKPYG--APEITKDGYKVMKGIKPEKPLHGroEL_Borrelia_garinii_YP_0730 VEKLSNAVKVTLGPKGRNVLIDKKFG--SPTVTKDGVSVAREIELENPFEGroEL_treponema_pallidum_YP_00 VEQISSAVKVTLGPKGRNVLLEKGYG--APTVTKDGVSVAKEVELEDPFEGroEL_Leptospira_interrogans_Y VNKLANAVKVTLGPKGRNVVIDKKFG--APTITKDGVTVAKEIELEDPLEGroEL_CH60_CHLPN_chlamydophila VKTLAEAVKVTLGPKGRHVVIDKSFG--SPQVTKDGVTVAKEIELEDKHEGroEL3_chlamydia_psittaci_AEG8 VKTLAEAVKVTLGPKGRHVVIDKSFG--SPQVTKDGVTVAKEIELEDKHEGroEL2_salmonella_enterica_typ VNVLADAVKVTLGPKGRNVVLDKSFG--APTITKDGVSVAREIELEDKFEGroEL_salmonella_typhi_TY2_U01 VNVLADAVKVTLGPKGRNVVLDKSFG--APTITKDGVSVAREIELEDKFEGroEL_E_coli_K12_AAC77103 VNVLADAVKVTLGPKGRNVVLDKSFG--APTITKDGVSVAREIELEDKFEGroEL_Yersinia_pestis_NP_99189 VNILADAVKVTLGPKGRNVVLDKSFG--SPTITKDGVSVAREIELEDKFEGroEL_haemophilus_influenzae_Y VNVLADAVKVTLGPKGRNVILDKSFG--APTITKDGVSVAREIELEDKFEGroEL_Legionella_YP_126086 VNALADAVQVTMGPRGRNVVLEKSYG--APTVTKDGVSVAKEIEFEHRFMGroEL_Burkholderia_multivorans VNVLADAVKVTLGPKGRNVLIERSFG--APTITKDGVSVAKEIELKDRFEGroEL_Bartonella_henselae_YP_0 VDILANAVKVTLGPKGRNVVIDKSFG--APRITKDGVSVAKEIELEDKFEGroEL_brucella_abortus_ZP_0587 VDILADAVKVTLGPKGRNVVIEKSFG--APRITKDGVSVAKEVELEDKFEGroEL_Mycobact_tuberc_CAA17397 LNALADAVKVTLGPKGRNVVLEKKWG--APTITNDGVSIAKEIELEDPYEGroEL_Mycobacterium_leprae_TN_ LNSLADAVKVTLGPKGRNVVLEKKWG--APTITNDGVSIAKEIELEDPYEGroEL_tropheryma_whipplei_NP_7 LNTLADTVKVTLGPRGRNVVLEKKWG--APVITNDGVTIAKEIELDDPYEGroEL_Staph_aureus_MRSA_YP_041 VDQLANAVKVTIGPKGRNVVLDKEFT--APLITNDGVTIAKEIELEDPYEGroEL_Listeria_monocytogenes_A VDQLANAVKVTLGPKGRNVVLEKKFG--SPLITNDGVTIAKEIELEDPFEGroEL_streptococcus_pneumoniae VDILADTVKVTLGPKGRNVVLEKSFG--SPLITNDGVTIAKEIELEDHFEGroEL_mycoplasma_penetrans_NP_ VQQLFDAVKVTMGPRGRNVLIQKSYG--APVITKDGVSVAKEVDLTNPIEHSP60_Leishmania_Strain_Friedl VENLVKAVGVTLGPKGRNVILEMPYA--CPKITKDGVTVAKSIEFEDSFEHSP60_Schistosoma_mansoni_XP_0 VDILADAVAVTMGPKGRNVIIESSWK--SPKITKDGVTVAKGIELKDKFQHsp60_human_mitochondria_NP_95 VDLLADAVAVTMGPKGRTVIIEQSWG--SPKVTKDGVTVAKSIDLKDKYKHSP60_Plasmodium_falciparum_XP CNKLADAVSVTLGPKGRNVIIEQSFG--SPKITKDGVTVAKSIEFNNKLAchaperonin60_Cryptosporidium_p ANDLADAVGVTLGPRGRNVVIEQGFGE-APKITKDGVTVAKAIQFGKGSVChaperonin60_Entamoeba_histoly IKKVADAVSVTLGPKGRTVIIDQPYG--NARVTKDGVSVAKALTFSDNTLChaperonin60_Giardia_lamblia_X IKTIADVVATTLGPRGRAVILADGSASGTTKVTKDGVSVARAINLSG-LE : ..* * **:* : : . :*:.* .: : : 150GroEL_orientia_tsutsugamushi_C NVGAQFVISVASKTADVAGDGTTTATVIADAAVRELNKAEVAGIDIQEVRGroEL_rickettsia_bellii_YP_537 NAGAQLLKSAATKAAEVAGDGTTTATVLARALAREGNKLVAAGYNPMDLKGroEL5_neoehrlichia_mikurensis QAIANIIAQSASQCNDKVGDGTTTCSILTAKVIEEVSKAKAAGADIISIKGroEL_anaplasma_phagocytophili AAIASIITQSASQCNDKVGDGTTTCSILTAKVIEEVSKAKAAGSDIVSIKGroEL_ehrlichia_deer_AB454077 LAIANIITQSASQCNDKVGDGTTTCSILTAKVIEEVSKAKAAGADIVCIKGroEL_Wolbachia_YP_198181 AAITSIFAQSCFQCNDKVGDGTTTCSILTSNMIMEALKSIAAGNDRVSIKGroEL_Borrelia_garinii_YP_0730 NMGAQLLKEVAIKTNDVAGDGTTTATVLAYAIAREGLKNVSSGINPIGIKGroEL_treponema_pallidum_YP_00 NMGAQLLKEVATKTNDVAGDGTTTATVLAYSMVREGLKAVAAGMTPLELKGroEL_Leptospira_interrogans_Y NMGAQMVKEVSTKTNDVAGDGTTTATILAQSIINEGLKNVTAGANPMSLKGroEL_CH60_CHLPN_chlamydophila NMGAQMVKEVASKTADKAGDGTTTATVLAEAIYSEGLRNVTAGANPMDLKGroEL3_chlamydia_psittaci_AEG8 NMGAQMVKEVASKTADKAGDGTTTATVLAEAIYSEGLRNVTAGANPMDLKGroEL2_salmonella_enterica_typ NMGAQMVKEVASKANDAAGDGTTTATVLAQSIITEGLKAVAAGMNPMDLKGroEL_salmonella_typhi_TY2_U01 NMGAQMVKEVASKANDAAGDGTTTATVLAQSIITEGLKAVAAGMNPMDLKGroEL_E_coli_K12_AAC77103 NMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKGroEL_Yersinia_pestis_NP_99189 NMGAQMVKEVASKANDAAGDGTTTATVLAQSIITEGLKAVAAGMNPMDLKGroEL_haemophilus_influenzae_Y NMGAQMVKEVASKANDAAGDGTTTATVLAQAIVNEGLKAVAAGMNPMDLKGroEL_Legionella_YP_126086 NMGAQMVKEVASKTSDTAGDGTTTATVLARSILVEGHKAVAAGMNPMDLKGroEL_Burkholderia_multivorans NMGAQVVKQVASKTADVAGDGTTTATVLAQAIVQEGMKHVAAGINPMDLKGroEL_Bartonella_henselae_YP_0 NMGAQMLREVASKTNDIAGDGTTTATVLGQAIVQEGVKAVAAGMNPMDLKGroEL_brucella_abortus_ZP_0587 NMGAQMLREVASKTNDTAGDGTTTATVLGQAIVQEGAKAVAAGMNPMDLKGroEL_Mycobact_tuberc_CAA17397 KIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREGLRNVAAGANPLGLKGroEL_Mycobacterium_leprae_TN_ KIGAELVKEVAKKTDDVAGDGTTTATVLAQALVKEGLRNVAAGANPLGLKGroEL_tropheryma_whipplei_NP_7 KIGAELVKEVAKKTDDVAGDGTTTSVVLAQAMVREGLKNVAAGADPISLRGroEL_Staph_aureus_MRSA_YP_041 NMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEGLKNVTSGANPVGLRGroEL_Listeria_monocytogenes_A NMGAKLVSEVASKTNDVAGDGTTTATVLAQAMIQEGLKNVTAGANPVGVRGroEL_streptococcus_pneumoniae NMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREGIKNVTAGANPIGIRGroEL_mycoplasma_penetrans_NP_ NMGAQLVKDVASKTADEAGDGTTTATVLAYGVFKEGLRNVISGANPIEIKHSP60_Leishmania_Strain_Friedl NLGANLVRQVAGLTNDNAGDGTTTATVLSGAIFKEGFRSVASGTNPMDLKHSP60_Schistosoma_mansoni_XP_0 NIGAKLVQDVANNTNEEAGDGTTTATVLARAIAKEGFEKISKGANPIEFRHsp60_human_mitochondria_NP_95 NIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRHSP60_Plasmodium_falciparum_XP NLGAQMVKQVAANTNDKAGDGTTTATILARSIFQQGCKAVDSGMNPMDLLchaperonin60_Cryptosporidium_p NLGAQLLKNVAISTNEEAGDGTTTATVLARAIFKSGCEKVDAGLNPMDLLChaperonin60_Entamoeba_histoly NVGGKIAKEVASKVNDRSGDGTTTATCLLRKVACEGVQAINTGLSGTDLLChaperonin60_Giardia_lamblia_X GVGADLIKDASLRTNTMAGDGTTTSLILSGKLVNEMNKYALSGLGNLQLL .. . . ***:**. : . . * . 200GroEL_orientia_tsutsugamushi_C KGAEKAVEAVIADVRKNSS--PVKNEEEIAQVATVSSNGDREIGEKIANAGroEL_rickettsia_bellii_YP_537 RGMDLAVNTVLEEVKKASK--KIDSQEEIAQVGTISSNGDKEIGEKIAKAGroEL5_neoehrlichia_mikurensis NGILKAKELVLESLLSMKR--DVSSEDEIAQVATISANGDKNIGSKIAQCGroEL_anaplasma_phagocytophili NGILKAKEAVLTALMSMRR--EVE-EDEIAQVATLSANGDKNIGSKIAQCGroEL_ehrlichia_deer_AB454077 EGVLKAKEAVLEALMSMKR--EVLSEEEIAQVATISANGDKNIGSKIAQCGroEL_Wolbachia_YP_198181 NGMQKAKDAVLEGITSMSRTIPLEKMDEVAQVAIISANGDKDIGNSIADAGroEL_Borrelia_garinii_YP_0730 KGIDHAVNLAAEKIRQSAK--KITTKEEIAQVASISANNDSYIGEKIAEAGroEL_treponema_pallidum_YP_00 RGMDKAVAIAVDDIKQNSK--GIKSNEEVAHVASVSANNDKEIGRILASAGroEL_Leptospira_interrogans_Y KGIDKAVTAAVESIQKRAV--KIENKKDIANVASISANNDNTIGNLIADAGroEL_CH60_CHLPN_chlamydophila RGIDKAVKVVVDELKKISK--PVQHHKEIAQVATISANNDSEIGNLIAEAGroEL3_chlamydia_psittaci_AEG8 RGIDKAVKVVVDQIKKISK--PVQHHKEIAQVATISANNDSEIGNLIAEAGroEL2_salmonella_enterica_typ RGIDKAVAAAVEELKALSV--PCSDSKAIAQVGTISANSDETVGKLIAEAGroEL_salmonella_typhi_TY2_U01 RGIDKAVAAAVEELKALSV--PCSDSKAIAQVGTISANSDETVGKLIAEAGroEL_E_coli_K12_AAC77103 RGIDKAVTAAVEELKALSV--PCSDSKAIAQVGTISANSDETVGKLIAEAGroEL_Yersinia_pestis_NP_99189 RGIDKAVIAAVEELKKLSV--PCSDSKAIAQVGTISANSDSTVGELIAQAGroEL_haemophilus_influenzae_Y RGIDKAVSAVVSELKNLSK--PCETAKEIEQVGTISANSDSIVGQLISQAGroEL_Legionella_YP_126086 RGIDKAVLAVTKKLQAMSK--PCKDSKAIAQVGTISANSDEAIGAIIAEAGroEL_Burkholderia_multivorans RGIDKAVGAVLDELRKLSR--PIATNKEIAQVGAISANSDEAIGKIIADAGroEL_Bartonella_henselae_YP_0 RGIDAAVDEVVANLFKKAK--KIQTSAEIAQVGTISANGAAEIGKMIADAGroEL_brucella_abortus_ZP_0587 RGIDLAVNEVVAELLKKAK--KINTSEEVAQVGTISANGEAEIGKMIAEAGroEL_Mycobact_tuberc_CAA17397 RGIEKAVEKVTETLLKGAK--EVETKEQIAATAAISA-GDQSIGDLIAEAGroEL_Mycobacterium_leprae_TN_ RGIEKAVDKVTETLLKDAK--EVETKEQIAATAAISA-GDQSIGDLIAEAGroEL_tropheryma_whipplei_NP_7 RGIEKSVAAVSKALLTSAK--EVETEAEIAACASISA-GDPQIGDIIAQAGroEL_Staph_aureus_MRSA_YP_041 QGIDKAVKVAVEALHENSQ--KVENKNEIAQVGAISA-ADEEIGRYISEAGroEL_Listeria_monocytogenes_A RGIEKAVATAIEELKAISK--PIESKESIAQVAAISS-GDEEVGKLIAEAGroEL_streptococcus_pneumoniae RGIETAVAAAVEALKNNAI--PVANKEAIAQVAAVSS-RSEKVGEYISEAGroEL_mycoplasma_penetrans_NP_ RGMDKTVNAIVNELNKSSK--KIARKDEIIQVATISANSDKKIGELIANAHSP60_Leishmania_Strain_Friedl RGIDLACREVLISLAEQSR--PVTSKSEITQVAMISANMDQEIGSLIGDAHSP60_Schistosoma_mansoni_XP_0 RGVMSAVDAVVKELKSLSK--PISTPEEIAKSQQYQPTVTKRLA-----IHsp60_human_mitochondria_NP_95 RGVMLAVDAVIAELKKQSK--PVTTPEEIAQVATISANGDKEIGNIISDAHSP60_Plasmodium_falciparum_XP RGINKGVEKVLEYLNSIKK--DVTTTEEIFNVASISANGDKNIGQLIADTchaperonin60_Cryptosporidium_p RGIKLGVEHVVNELDLLSQ--PVKSHDDILNVATISANGDSIVGSLIAQAChaperonin60_Entamoeba_histoly KGISIAKDIVLKEITKQSK---PTLKEDIISVARVSANNDEKIGEMVGDIChaperonin60_Giardia_lamblia_X QALNSAGVDCLQSLRKQSR--AIESNKMLYSVATIAANNDPKIGKVVSDA .. : : . :. 1st apical loop 250GroEL_orientia_tsutsugamushi_C MKQVGQEGVITVEDSKNFN-FEVEVVKGMRFDRGYISQYFATNREKMITEGroEL_rickettsia_bellii_YP_537 MEEVGKEGVITVEEAKNFS-FDVEVVKGMMFDRGYLSPYFVTNSEKMVAEGroEL5_neoehrlichia_mikurensis VKEVGKDGVITVEESKGFKELEVEKTDGMQFDRGYLSPYFVTNAEKMLIEGroEL_anaplasma_phagocytophili VKEVGKDGVITVEESKGFKDLEVEKTDGMQFDRGYLSPYFVTNAEKMLVEGroEL_ehrlichia_deer_AB454077 VQEVGKDGVITVEESKGFKELDVEKTDGMQFDRGYLSPYFVTNSEKMLVEGroEL_Wolbachia_YP_198181 VKKVGKEGVITVEESKGSKELEVELTTGMQFDRGYLSPYFITSNEKMIVEGroEL_Borrelia_garinii_YP_0730 MDKVGKDGVITVEESKTFD-TTISYVEGMQFDRGYLSPYFSTNKENMSVSGroEL_treponema_pallidum_YP_00 IEKVGNDGVIDVDEAQTME-TVTEFVEGMQFDRGYISSYFVTDRDRMETVGroEL_Leptospira_interrogans_Y MDKVGKDGVITVEEAKSIE-TTLDVVEGMQFDRGYISPYMVTDAESMVATGroEL_CH60_CHLPN_chlamydophila MEKVGKNGSITVEEAKGFE-TVLDVVEGMNFNRGYLSSYFSTNPETQECVGroEL3_chlamydia_psittaci_AEG8 MEKVGKNGSITVEEAKGFE-TVLDVVEGMNFNRGYLSSYFSTNPETQECVGroEL2_salmonella_enterica_typ MDKVGKEGVITVEDGTGLQ-DELDVVEGMQFDRGYLSPYFINKPETGAVEGroEL_salmonella_typhi_TY2_U01 MDKVGKEGVITVEDGTGLQ-DELDVVEGMQFDRGYLSPYFINKPETGAVEGroEL_E_coli_K12_AAC77103 MDKVGKEGVITVEDGTGLQ-DELDVVEGMQFDRGYLSPYFINKPETGAVEGroEL_Yersinia_pestis_NP_99189 MEKVGKEGVITVEEGSGLQ-DELDVVEGMQFDRGYLSPYFINKPETGSIEGroEL_haemophilus_influenzae_Y MEKVGKEGVITVEDGTGLE-DELDVVEGMQFDRGYLSPYFINKPETATVEGroEL_Legionella_YP_126086 MEKVGKEGVITVEDGNGLE-NELSVVEGMQFDRGYISPYFINNQQNMSCEGroEL_Burkholderia_multivorans MERVGKEGVITVEDGKSLE-NELEVVEGMQFDRGYVSPYFINDPEKQAAYGroEL_Bartonella_henselae_YP_0 MEKVGNEGVITVEEAKTAE-TELEVVEGMQFDRGYLSPYFVTNAEKMVADGroEL_brucella_abortus_ZP_0587 MQKVGNEGVITVEEAKTAE-TELEVVEGMQFDRGYLSPYFVTNPEKMVADGroEL_Mycobact_tuberc_CAA17397 MDKVGNEGVITVEESNTFG-LQLELTEGMRFDKGYISGYFVTDPERQEAVGroEL_Mycobacterium_leprae_TN_ MDKVGNEGVITVEESNTFG-LQLELTEGMRFDKGYISGYFVTDAERQEAVGroEL_tropheryma_whipplei_NP_7 LEKVGKEGVVTVEESNTFG-TELEITEGMRFDKGYLSAYFVTDAERQETVGroEL_Staph_aureus_MRSA_YP_041 MEKVGNDGVITIEESNGLN-TELEVVEGMQFDRGYQSPYMVTDSDKMVAEGroEL_Listeria_monocytogenes_A MERVGNDGVITIEESKGFA-TELDVVEGMQFDRGYTSPYMVTDSDKMEAVGroEL_streptococcus_pneumoniae MEKVGKDGVITIEESRGME-TELEVVEGMQFDRGYLSQYMVTDSEKMVADGroEL_mycoplasma_penetrans_NP_ MEKVGSDGVITVEEAKGIN-DELTVVEGMQFDRGYISPYFVTDTNKMIAKHSP60_Leishmania_Strain_Friedl MQQVGKDGVITTQEGRSLN-TELELVEGMSFERGYTSPYFVTNTKAQRCEHSP60_Schistosoma_mansoni_XP_0 MKKVGNDGTITVKDGKTLH-DELEFIEGMKFDRGYISPYFLNTEKGARCEHsp60_human_mitochondria_NP_95 MKKVGRKGVITVKDGKTLN-DELEIIEGMKFDRGYISPYFINTSKGQKCEHSP60_Plasmodium_falciparum_XP MKKVGKEGTITVTEGKTLQ-HELEIVEGIKFDRGYISPYFINNSKDQKVEchaperonin60_Cryptosporidium_p YSKVGRHGTINIEEGNTTQ-SELEIVEGLKLDKGYISPYFITNQKYQKVEChaperonin60_Entamoeba_histoly FGKIGRDGAVDIETGKGTK-DIVNIVEGMVLDQGFLSRYFTTDEKNTKVDChaperonin60_Giardia_lamblia_X FAAVGREGTITVEDG-YTDIDTLNVTDGCSIPSGFLSPYFALGGSR-YLE :* .* : . . : *: * *: . apical H helix, apical I helix 300 HHHHHHHHHH IIIIIIGroEL_orientia_tsutsugamushi_C FENPYILLLDQKVSTV-QPLVPVLEAVAHTGK-PLVLIADDVDGEALTALGroEL_rickettsia_bellii_YP_537 LENPYILLFEKKLSNL-QPMLPILEAVVQSQR-PLLIIAEDVEGEALATLGroEL5_neoehrlichia_mikurensis FENPYILLTEKKLNII-QPILPILENIARSGR-PLLIIAEDVEGEALSTLGroEL_anaplasma_phagocytophili FENPYIFLTEKKINLV-QSILPILENVARSGR-PLLIIAEDVEGEALSTLGroEL_ehrlichia_deer_AB454077 FENPYILLTEKKLNII-QPILPILENVARSGR-PLLIIAEDVEGEALSTLGroEL_Wolbachia_YP_198181 FDDPYLLITEKKLSII-QPLLPILEAVVKSGK-PLLIIAEDIEGEALSTLGroEL_Borrelia_garinii_YP_0730 FDDAFILIYEKKISSI-KELLPVLEKVLGTNK-PLLIIAEDIEGDALAALGroEL_treponema_pallidum_YP_00 YENPYILIYDKSISTM-KDLLPLLEKIAQTGR-PLLIIAEDVEGEALATLGroEL_Leptospira_interrogans_Y LNDPFILIYDKKISSM-KDLIHILEKVAQAGK-PLVIISEEVEGEALATIGroEL_CH60_CHLPN_chlamydophila LEDALILIYDKKISGI-KDFLPVLQQVAESGR-PLLIIAEEIEGEALATLGroEL3_chlamydia_psittaci_AEG8 LEEALVLIYDKKISGI-KDFLPVLQQVAESGR-PLLIIAEDIEGEALATLGroEL2_salmonella_enterica_typ LESPFILLADKKISNI-REMLPVLEAVAKAGK-PLLIIAEDVEGEALATLGroEL_salmonella_typhi_TY2_U01 LESPFILLADKKISNI-REMLPVLEAVAKAGK-PLLIIAEDVEGEALATLGroEL_E_coli_K12_AAC77103 LESPFILLADKKISNI-REMLPVLEAVAKAGK-PLLIIAEDVEGEALATLGroEL_Yersinia_pestis_NP_99189 LESPFILLADKKISNI-REMLPVLEAVAKAGK-PLLIIAEDVEGEALATLGroEL_haemophilus_influenzae_Y LDNPYLLLVDKKISNI-RELLPVLEGVAKAGK-PLLIIAEDVEGEALATLGroEL_Legionella_YP_126086 LEHPFILLVDKKVSSI-REMLSVLEGVAKSGR-PLLIIAEDVEGEALATLGroEL_Burkholderia_multivorans LDDPLILLHDKKISSI-RDLLPILEAASKAGK-PLLIVAEDVDGEALATLGroEL_Bartonella_henselae_YP_0 LDDPYILIHEKKLSNL-QSLLPVLEAVVQSGK-PLLIIAEDVEGEALATLGroEL_brucella_abortus_ZP_0587 LEDAYILLHEKKLSNL-QALLPVLEAVVQTSK-PLLIIAEDVEGEALATLGroEL_Mycobact_tuberc_CAA17397 LEDPYILLVSSKVSTV-KDLLPLLEKVIGAGK-PLLIIAEDVEGEALSTLGroEL_Mycobacterium_leprae_TN_ LEEPYILLVSSKVSTV-KDLLPLLEKVIQAGK-SLLIIAEDVEGEALSTLGroEL_tropheryma_whipplei_NP_7 FENPYILICDSKISSV-KDLLPVVDKVIQSGK-QLLIIAEDVDGEALATLGroEL_Staph_aureus_MRSA_YP_041 LERPYILVTDKKISSF-QDILPLLEQVVQSNR-PILIVADEVEGDALTNIGroEL_Listeria_monocytogenes_A LEKPYILITDKKINNI-QEILPVLEQVVQQGR-PMLIIAEDVEGEAQATLGroEL_streptococcus_pneumoniae LENPYILITDKKISNI-QEILPLLESILQSNR-PLLIIADDVDGEALPTLGroEL_mycoplasma_penetrans_NP_ LENPYILITDKKVSSI-KDILPILEEIMKTGR-PLLIIADDVDGEALTTLHSP60_Leishmania_Strain_Friedl LENALVYVANRKLTSV-AHILPALNYAIQQKR-PLLVIAEDVEGEAMHTFHSP60_Schistosoma_mansoni_XP_0 FQDAFVLFSEKKINSI-QTLLPALELCHQQKR-PLLIIAEDVEGEALTALHsp60_human_mitochondria_NP_95 FQDAYVLLSEKKISSI-QSIVPALEIANAHRK-PLVIIAEDVDGEALSTLHSP60_Plasmodium_falciparum_XP LDKPYILIHEKKISTV-KSLLPVLEHVLQNQS-SLLVIAEDVDSDALATLchaperonin60_Cryptosporidium_p LENPYILISQGKISSL-KSILPILEFCISSRS-PLLIIAEEIEGEALTALChaperonin60_Entamoeba_histoly IRNTDVIVCDYKLSSS-QSVVPLLELCLKRKR-PLVVISDTIDGDALTTLChaperonin60_Giardia_lamblia_X LTNPLVVITDTVLSSA-APLVSILERCVKEKR-PLLIIASDVTGDALSTL . : . . :. .: :: :::::. . : : 2nd apical loop 350 IIIIIII GroEL_orientia_tsutsugamushi_C ILNNLKGSIKVVAVKAPGFGDRKKEMLEDIAILTNG-EVITEQLGIKLEKGroEL_rickettsia_bellii_YP_537 VVNRLRGGLKVAAVKAPGFGDRRKAMMEDIAILTNG-ELITEDLGMKLENGroEL5_neoehrlichia_mikurensis VLNKLRGGLHVAAVKAPGFGDRRKDMLGDIAILTGAKYVINDELAVKMEDGroEL_anaplasma_phagocytophili VLNKLRGGLQVAAVKAPGFGDRRKDMLGDIAVIVGAKYVVNDELAVKMEDGroEL_ehrlichia_deer_AB454077 VLNKLRGGLHVAAVKAPGFGDRRKDMLGDIAILTGAKHVISDDLAIKMEDGroEL_Wolbachia_YP_198181 VINKLRGGLKVTAVKAPGFGDRRKEMLEDIAALTGAKYVIKDELGIKMEDGroEL_Borrelia_garinii_YP_0730 VLNSVRGALKVCAIKSPGFGDRRKAMLEDIAVLTGG-VLISEELGITLETGroEL_treponema_pallidum_YP_00 VVNSLRGTLKTCAVKAPGFGDRRKEMLEDIAILSGG-QVISEDLGLKLESGroEL_Leptospira_interrogans_Y VVNTLRKTISCVAVKAPGFGDRRKSMLEDIAILTGG-QVISEDLGMKLENGroEL_CH60_CHLPN_chlamydophila VVNRLRAGFRVCAVKAPGFGDRRKAMLEDIAILTGG-QLVSEELGMKLENGroEL3_chlamydia_psittaci_AEG8 VVNRLRAGFRVCAVKAPGFGDRRKAMLEDIAILTGG-QLISEELGMKLENGroEL2_salmonella_enterica_typ VVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGG-TVISEEIGMELEKGroEL_salmonella_typhi_TY2_U01 VVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGG-TVISEEIGMELEKGroEL_E_coli_K12_AAC77103 VVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGG-TVISEEIGMELEKGroEL_Yersinia_pestis_NP_99189 VVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTAG-TVISEEIGLELEKGroEL_haemophilus_influenzae_Y VVNTMRGIVKVAAVKAPGFGDRRKAMLQDIAILTAG-TVISEEIGMELEKGroEL_Legionella_YP_126086 VVNNMRGIVKVCAVKAPGFGDRRKAMLQDIAILTKG-QVISEEIGKSLEGGroEL_Burkholderia_multivorans VVNAMRGILKVAAVKAPGFGDRRKAMLEDIAILTGA-TVISEETGKQLEKGroEL_Bartonella_henselae_YP_0 VVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTSG-QVISEDVGIKLENGroEL_brucella_abortus_ZP_0587 VVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTGG-QVISEDLGIKLESGroEL_Mycobact_tuberc_CAA17397 VVNKIRGTFKSVAVKAPGFGDRRKAMLQDMAILTGG-QVISEEVGLTLENGroEL_Mycobacterium_leprae_TN_ VVNKIRGTFKSVAVKAPGFGDRRKAMLQDMAILTGA-QVISEEVGLTLENGroEL_tropheryma_whipplei_NP_7 VVNKIRGIFKSVAVKAPGFGDRRKMMLQDIAVLTGG-QVISEEVGLKLENGroEL_Staph_aureus_MRSA_YP_041 VLNRMRGTFTAVAVKAPGFGDRRKAMLEDLAILTGA-QVITDDLGLDLKDGroEL_Listeria_monocytogenes_A VLNKLRGTFNVVAVKAPGFGDRRKAMLEDIAILTGG-QVITEDLGLELKTGroEL_streptococcus_pneumoniae VLNKIRGTFNVVAVKAPGFGDRRKAMLEDIAILTGG-TVITEDLGLELKDGroEL_mycoplasma_penetrans_NP_ VVNKMRGVFNVVAVKAPEFGDKRKQVLEDIAILTGG-SFVTDDLGISFDKHSP60_Leishmania_Strain_Friedl LYNKIQGRISGCAVKAPGFGDMRINQLQDIAVFTGS-QMISEDLGLSLDQHSP60_Schistosoma_mansoni_XP_0 VLNRLKLGLQVCAVKAPGFGDNRKNTLKDMAVATGGIVFGDEADMYKLEDHsp60_human_mitochondria_NP_95 VLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDHSP60_Plasmodium_falciparum_XP IVNKLRLGLKICAVKAPGFGEHRKALIHDIAVMTGAKVITEETG-LKLDDchaperonin60_Cryptosporidium_p ILNKLQLNLKVCAVKAPGFGDHRKQILEDISVSVGAKIIQEEFSNAKLDQChaperonin60_Entamoeba_histoly VLNKLRG-LPIAAVRAPGFGETRKGILHDIGIITGA-TVISNEAGKKIEEChaperonin60_Giardia_lamblia_X AINTLKGTVRCCAVRAPGYGDVKKGVLEDLAAVVGIPTYISDELHTASAP * :: . *:::* :*: : : *:. : 400GroEL_orientia_tsutsugamushi_C VN--DTSK-LGTANRVIVTKDHTTIVHDKNNSDIEKKVNSRCEQIREAIKGroEL_rickettsia_bellii_YP_537 VS--LKS--LGHAKRVTISKENTVIVDGSGD---KKNIEERVLQIKSHIAGroEL5_neoehrlichia_mikurensis LT--LDD--LGTAKNIRITKDTTTLIGSVDSNS--SNVQSRINQIKMQIDGroEL_anaplasma_phagocytophili IA--LSD--LGTAKSVRITKDATTIIGSVDSSS--ESIASRTNQIKAQIEGroEL_ehrlichia_deer_AB454077 LT--LAE--LGTAKNIRITKDTTTIIGSVDNSS--ANVQNRINQIKMQIEGroEL_Wolbachia_YP_198181 LT--LED--LGTAKNVKVTKDNTTIVSGS-SDS--DRVKARVEQIKSQIEGroEL_Borrelia_garinii_YP_0730 VE--IEQ--LGQAKTIKVDKDNTTIIN-TGNKE---QIKERSELIKKQIEGroEL_treponema_pallidum_YP_00 AD--IAL--LGQAKSVKVDKENTTIIDGSGKSK---DIKDRIEQIKKQIEGroEL_Leptospira_interrogans_Y TT--LQM--LGRANKVTVDKENTTIIEGKGQTK---EIQGRIGQIKKQIEGroEL_CH60_CHLPN_chlamydophila TT--LAM--LGKAKKVIVTKEDTTIVEGLGNKP---DIQARCDNIKKQIEGroEL3_chlamydia_psittaci_AEG8 TT--LSM--LGKAKKVIVSKEDTTIVEGLGNKE---DIEARCENIKKQIEGroEL2_salmonella_enterica_typ AT--LED--LGQAKRVVINKDTTTIIDGVGEEA---AIQGRVAQIRQQIEGroEL_salmonella_typhi_TY2_U01 AT--LED--LGQAKRVVINKDTTTIIDGVGEEA---AIQGRVAQIRQQIEGroEL_E_coli_K12_AAC77103 AT--LED--LGQAKRVVINKDTTTIIDGVGEEA---AIQGRVAQIRQQIEGroEL_Yersinia_pestis_NP_99189 TT--LED--LGQAKRVVINKDTTIIIDGVGDEA---AIQGRVAQIRQQIEGroEL_haemophilus_influenzae_Y AT--LED--LGQAKRVVINKDNTTIIDGIGDEA---QIKGRVAQIRQQIEGroEL_Legionella_YP_126086 AT--LED--LGSAKRIVVTKENTTIIDGEGKAT---EINARITQIRAQMEGroEL_Burkholderia_multivorans AT--LED--LGRAKRVEVRKDDTIIIDGAGDPA---RIDARVKAIRVQIDGroEL_Bartonella_henselae_YP_0 VT--LDM--LGRAKKVNISKENTTIIDGAGQKS---EINARVNQIKVQIEGroEL_brucella_abortus_ZP_0587 VT--LDM--LGRAKKVSISKENTTIVDGAGQKA---EIDARVGQIKQQIEGroEL_Mycobact_tuberc_CAA17397 AD--LSL--LGKARKVVVTKDETTIVEGAGDTD---AIAGRVAQIRQEIEGroEL_Mycobacterium_leprae_TN_ TD--LSL--LGKARKVVMTKDETTIVEGAGDTD---AIAGRVAQIRTEIEGroEL_tropheryma_whipplei_NP_7 AT--LDL--LGCARKVVVSKDETTIVDGAGSSD---QIAGRVSQIRKELEGroEL_Staph_aureus_MRSA_YP_041 AT--IDM--LGTASKVEVTKDNTTVVDGDGDEN---SIDARVSQLKSQIEGroEL_Listeria_monocytogenes_A AT--VDQ--LGTANKVVVTKDDTTIVEGAGDST---QISARVNQIRAQMEGroEL_streptococcus_pneumoniae AT--IEA--LGQAARVTVDKDSTVIVEGAGNPE---AISHRVAVIKSQIEGroEL_mycoplasma_penetrans_NP_ VT--LQD--LGQAESVVIDKDNSTIVKGKGLES---QIKERISKIKTAIEHSP60_Leishmania_Strain_Friedl ND--FSERFLGTCRKVTVSRDECILMEGGGSAI---AVEERVQMIKDMISHSP60_Schistosoma_mansoni_XP_0 VQ--LQD--LGRVAEVVVTKDDCLLMRGRGSKT---DVDKRIAQIKEEMEHsp60_human_mitochondria_NP_95 VQ--PHD--LGKVGEVIVTKDDAMLLKGKGDKA---QIEKRIQEIIEQLDHSP60_Plasmodium_falciparum_XP P---QVVSYLGKAKSINVTKDSTLIMEGEGKKE---EINERCESIRNAIKchaperonin60_Cryptosporidium_p MNSNQIQEFLGKCKSISVSKDETIITQGQGSPK---DVKDTISLLKSQIEChaperonin60_Entamoeba_histoly VT--EKD--LGKIGHFVSTKDETIITGGAGSKA---EVLARINELKNAKEChaperonin60_Giardia_lamblia_X GSAVLSN--IGSCHKAIITPANTVLHFNDDKNCN-SLIRGRVAGLRSLLE :* : : 450GroEL_orientia_tsutsugamushi_C DT--TSDYEKEKLQERLAKLRNGVAVLKVGGATEVEQKERKDRVEDALHAGroEL_rickettsia_bellii_YP_537 ET--TSDYDKEKLQERLAKLSGGVAVLKVGGATEVEVKERKDRVEDALAAGroEL5_neoehrlichia_mikurensis TS--TSDYDKEKLKERLAKLSGGVAVLKVGGSSEVEVKERKDRVE-----GroEL_anaplasma_phagocytophili NS--SSDYDKEKLRERLAKLSGGVAVLKVGGSSEVEVKERKDRVEDALHAGroEL_ehrlichia_deer_AB454077 AS--TSDYDKEKLRERLAKLSGGVAVLKVGGSSEVEVKERKDRVEDALHAGroEL_Wolbachia_YP_198181 TS--TSDYDKEKLRERLAKLSGGVAVLKVGGVTEVEVKERRDRVEDALHAGroEL_Borrelia_garinii_YP_0730 DS--TSEYDKEKLQERLAKLVGGVAVINVGAVTEVELKEKKHRVEDALSAGroEL_treponema_pallidum_YP_00 AS--TSDYDSEKLKERLAKLSGGVAVIKIGAVTEVEMKEKKHRVEDALNAGroEL_Leptospira_interrogans_Y DT--TSEYDREKLQERLAKLAGGVAVIHVGAATEVEMKEKKARVEDALSAGroEL_CH60_CHLPN_chlamydophila DS--TSDYDKEKLQERLAKLSGGVAVIRVGAATEIEMKEKKDRVDDAQHAGroEL3_chlamydia_psittaci_AEG8 DS--TSDYDKEKLQERLAKLSGGVAVIRVGAATEIEMKEKKDRVDDAQHAGroEL2_salmonella_enterica_typ EA--TSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHAGroEL_salmonella_typhi_TY2_U01 EA--TSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHAGroEL_E_coli_K12_AAC77103 EA--TSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHAGroEL_Yersinia_pestis_NP_99189 DA--TSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHAGroEL_haemophilus_influenzae_Y ES--TSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVDDALHAGroEL_Legionella_YP_126086 ET--TSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHAGroEL_Burkholderia_multivorans EA--TSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVDDALHAGroEL_Bartonella_henselae_YP_0 ET--TSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDALNAGroEL_brucella_abortus_ZP_0587 ET--TSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDALNAGroEL_Mycobact_tuberc_CAA17397 NS--DSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKHRIEDAVRNGroEL_Mycobacterium_leprae_TN_ NS--DSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKHRIEDAVRNGroEL_tropheryma_whipplei_NP_7 NS--DSDYDREKLQERLAKLSGGVAVIRSGAATEVELKERKHRIEDAVRNGroEL_Staph_aureus_MRSA_YP_041 ET--ESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSGroEL_Listeria_monocytogenes_A ET--TSEFDREKLQERLAKLAGGVAVVKVGAATETELKERKLRIEDALNSGroEL_streptococcus_pneumoniae TT--TSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKLRIEDALNAGroEL_mycoplasma_penetrans_NP_ MT--DSDYDKDSLRNRLAKLNKGVAVIKVGAVSEVELKEKKDRVDDALSAHSP60_Leishmania_Strain_Friedl AE--DHEYNRERLVERLAKLSGGVAVIKVGGASEVEINEKKDRIIDALNAHSP60_Schistosoma_mansoni_XP_0 AS--NSEYEKEKMHERLAKLSNGVAVIKVGGSSEVEVSEKKDRYTDALNAHsp60_human_mitochondria_NP_95 VT--TSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNAHSP60_Plasmodium_falciparum_XP MN--TSDYEKEKLQERLAKITGGVALIKVGGISEVEVNEIKDRIQDALCAchaperonin60_Cryptosporidium_p ENQKLTDYDKEKLRERLARLTGRVALIKIGGYSDTEISELKDRFIDALNAChaperonin60_Entamoeba_histoly VS--DSSYEKEKLEGRIARLTGGVAVISVGGSSEAEVGERKDRIEDAVCAChaperonin60_Giardia_lamblia_X SNN-LTNYQRSKLNERIGRLLGKVCTIRIGAKTELEAEEKKDRYIDSLSA . : . : *:.:: :. : *. :: * * : 500GroEL_orientia_tsutsugamushi_C TRAAVEEGIVPGGGVALFYASR--VLDS--LKFD----------------GroEL_rickettsia_bellii_YP_537 TRAAVEEGVVAGGGVTLLHASQ--ALKN--LKVD----------------GroEL5_neoehrlichia_mikurensis --------------------------------------------------GroEL_anaplasma_phagocytophili TRAAVEEGVVPGGGAALLYALS--SLDG--LKGK----------------GroEL_ehrlichia_deer_AB454077 TRACC---------------------------------------------GroEL_Wolbachia_YP_198181 TRAAIEEGIVPGGGVALLYASS--ALDK--LKGG----------------GroEL_Borrelia_garinii_YP_0730 TRAAVEEGVVPGGGSTLIEVAM--YLDTIDTSKL----------------GroEL_treponema_pallidum_YP_00 TRAAIEEGIVAGGGLALIQAAA--ALEKADLSGL----------------GroEL_Leptospira_interrogans_Y TRAAVEEGIVPGGGLTLLKAQE--AVGSLKLDG-----------------GroEL_CH60_CHLPN_chlamydophila TIAAVEEGILPGGGTALVRCIP--TLEAFLPMLA----------------GroEL3_chlamydia_psittaci_AEG8 TLAAVEEGILPGGGTALVRCIP--TLEAFIPVLT----------------GroEL2_salmonella_enterica_typ TRAAVEEGVVAGGGVALIRVAS--KIAD--LKGQ----------------GroEL_salmonella_typhi_TY2_U01 TRAAVEEGVVAGGGVALIRVAS--KIAD--LKGQ----------------GroEL_E_coli_K12_AAC77103 TRAAVEEGVVAGGGVALIRVAS--KLAD--LRGQ----------------GroEL_Yersinia_pestis_NP_99189 TRAAVEEGVVAGGGVALIRAAH--AIAG--LKGD----------------GroEL_haemophilus_influenzae_Y TRAAVEEGIVAGGGVALVRAAA--KVAAS-LKGD----------------GroEL_Legionella_YP_126086 TRAAVEEGIVAGGGVALIRAQK--ALDS--LKGD----------------GroEL_Burkholderia_multivorans TRAAVEEGIVPGGGVALLRARA--ALAD--IKGA----------------GroEL_Bartonella_henselae_YP_0 TRAAVEEGIVAGGGTALLRAAN--ALTV---KGS----------------GroEL_brucella_abortus_ZP_0587 TRAAVEEGIVAGGGTALLRAST--KITA---KGV----------------GroEL_Mycobact_tuberc_CAA17397 AKAAVEEGIVAGGGVTLLQAAP--TLDELKLEG-----------------GroEL_Mycobacterium_leprae_TN_ AKAAVEEGIVAGGGVTLLQAAP--ALDKLKLTG-----------------GroEL_tropheryma_whipplei_NP_7 AKAAVEEGIVAGGGAALLQSGTS-ALKDLQLTS-----------------GroEL_Staph_aureus_MRSA_YP_041 TRAAVEEGIVAGGGTALVNVYQ--KVSEIEAEG-----------------GroEL_Listeria_monocytogenes_A TRAAVEEGIVAGGGTALVSIYN--KVAALEAEG-----------------GroEL_streptococcus_pneumoniae TRAAVEEGIVAGGGTALANVIP--AVATLELTG-----------------GroEL_mycoplasma_penetrans_NP_ TKAAIEEGIVIGGGAALVHVSKRINVNTLNLIG-----------------HSP60_Leishmania_Strain_Friedl TRAAVSEGILAGGGTGLLMASLR-LESISKDRRL----------------HSP60_Schistosoma_mansoni_XP_0 TRAAIEEGIVPGGGTALLRCIP--ILKS--LESK----------------Hsp60_human_mitochondria_NP_95 TRAAVEEGIVLGGGCALLRCIP--ALDS--LTPA----------------HSP60_Plasmodium_falciparum_XP TKAAVEEGIVPGGGSALLFASK--ELDSVQTD------------------chaperonin60_Cryptosporidium_p TKCAIEQGIVPGGGSALLWASR--NLGKLYSQSPPPGKTLTPSQSSSNESChaperonin60_Entamoeba_histoly VKAALAEGIVPGGGVALIRAGS--SLDKIRSQNW----------------Chaperonin60_Giardia_lamblia_X ARAALEGGLLPGGGVAFLRAAQVMERKLAEGKVADP-------------- 550GroEL_orientia_tsutsugamushi_C ----NEDQRVGINIIKKVLEAPVRQIVKNAGGKEDVVVN-ELSK--STDKGroEL_rickettsia_bellii_YP_537 ----NKDQQAGIELVIEALKDPIKQIVENAGENGGVVVG-KLLE--HKDKGroEL5_neoehrlichia_mikurensis --------------------------------------------------GroEL_anaplasma_phagocytophili ----NDDEQWGIDIIRRAACAPIKRIIKNSGSEEAPCVIQHLLK--QNDKGroEL_ehrlichia_deer_AB454077 --------------------------------------------------GroEL_Wolbachia_YP_198181 ----SDEEQIGINIIKKVLSAPIKRLVKNAGLES-AVIIDHLTK--QNDKGroEL_Borrelia_garinii_YP_0730 ----SYEEKQGFEIVKRSLEEPMRQIISNAGFEGSIYIHQIKT----EKKGroEL_treponema_pallidum_YP_00 ----TPDEAVGFKIVRRALEEPIRQISENAGIDGAVVAEKAK-----EKRGroEL_Leptospira_interrogans_Y ------DEATGAKIIFRALEEPIRMITSNAGLEGSVIVEHAKA----KKGGroEL_CH60_CHLPN_chlamydophila ----NEDEAIGTRIILKALTAPLKQIASNAGKEGAIICQQVLA----RSAGroEL3_chlamydia_psittaci_AEG8 ----NEDEQIGARIVLKALSAPLKQIAANAGKEGAIICQQVLS----RSSGroEL2_salmonella_enterica_typ ----NEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKG----GDGGroEL_salmonella_typhi_TY2_U01 ----NEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKG----GDGGroEL_E_coli_K12_AAC77103 ----NEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKG----GDGGroEL_Yersinia_pestis_NP_99189 ----NEDQNVGIKVALRAMESPLRQIVVNAGEEASVIANKVKA----GEGGroEL_haemophilus_influenzae_Y ----NEEQNVGIKLALRAMEAPLRQIVTNAGEEASVVASAVKN----GEGGroEL_Legionella_YP_126086 ----NDDQNMGINILRRAIESPMRQIVTNAGYEASVVVNKVAE----HKDGroEL_Burkholderia_multivorans ----NADQDAGIRIVLRALEAPLRVIVSNAGEEPSVVIAKVLE----GKGGroEL_Bartonella_henselae_YP_0 ----NPDQEAGINIVRRALQAPARQIATNAGEEAAIIVGKVLEN---NADGroEL_brucella_abortus_ZP_0587 ----NADQEAGINIVRRAIQAPARQITTNAGEEASVIVGKILEN---TSEGroEL_Mycobact_tuberc_CAA17397 ------DEATGANIVKVALEAPLKQIAFNSGLEPGVVAEKVRN----LPAGroEL_Mycobacterium_leprae_TN_ ------DEATGANIVKVALEAPLKQIAFNSGMEPGVVAEKVRN----LSVGroEL_tropheryma_whipplei_NP_7 ------EEAVGRNIVRSAIEAPLRQISLNAGLEPGVVVGKVSS----LPQGroEL_Staph_aureus_MRSA_YP_041 ------DIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKN----AEPGroEL_Listeria_monocytogenes_A ------DVETGINIVLRSLEEPVRQIAHNAGLEGSVIVERLKH----EAVGroEL_streptococcus_pneumoniae ------DEATGRNIVLRALEEPVRQIAHNAGFEGSIVIDRLKN----AELGroEL_mycoplasma_penetrans_NP_ ------DEKIGYQIVMSAIMSPISQIVSNAGFDKGVVINEILKA---TNPHSP60_Leishmania_Strain_Friedl ----PPDIRTGVNIVKKAIGLPARYIANNAGVEGSVVAGKVLAR---KDPHSP60_Schistosoma_mansoni_XP_0 ----NEDQRTGVQIVLRALSTPCYTIAHNAGVNASVVVEKVMG----MGQHsp60_human_mitochondria_NP_95 ----NEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQ----SSSHSP60_Plasmodium_falciparum_XP ----NYDQRVGVNIIKDACKAPIKQIAENAGHEGSVVAGNILK--EKNS-chaperonin60_Cryptosporidium_p NPIRNYDMAMGVKIVQDACKVPCHLISSNAGFDGSVIVGELVKVFSKGSKChaperonin60_Entamoeba_histoly ------AEKVGIDIVRKVTEEPTRIIARNAGIDGGIVIQKIKEG----TGChaperonin60_Giardia_lamblia_X ------VTIAAHKALIAALHEPARIIAESAGASGHVVAEAIKNS---PDN 600GroEL_orientia_tsutsugamushi_C NRGFDARTMQYVDMIKAGIVDPTKVVRTALQDAFSVASLVIATSAMITD-GroEL_rickettsia_bellii_YP_537 NFGFNAQDMQYVDMIKAGIIDPAKVVRTALQDAASVASLIITTETLIVD-GroEL5_neoehrlichia_mikurensis --------------------------------------------------GroEL_anaplasma_phagocytophili ELIYNVDTMNYANAFTSGVMDPLKVVRIAFDLAVSLAAVFMTLNAVVVD-GroEL_ehrlichia_deer_AB454077 --------------------------------------------------GroEL_Wolbachia_YP_198181 ELIYNVEAMNYANAFTAGVIDPAKVVRIAFETAISVASVLITTESMIVD-GroEL_Borrelia_garinii_YP_0730 GLGFDASSFKWVNMIESGIIDPAKVTRSALQNAASIAGLLLTTECAITD-GroEL_treponema_pallidum_YP_00 GIGFDASKMEWVDMIKVGIIDPAKVTRSALQNAASVSGLLLTTECAIAA-GroEL_Leptospira_interrogans_Y NEGFNALTMVWEDMIQAGVVDPAKVVRSALQNAASIGSMILTTEVTITD-GroEL_CH60_CHLPN_chlamydophila NEGYDALRDAYTDMIDAGILDPTKVTRSALESAASIAGLLLTTEALIAD-GroEL3_chlamydia_psittaci_AEG8 NEGYDALRDAYTDMIEAGILDPTKVTRCALESAASVAGLLLTTEALIAD-GroEL2_salmonella_enterica_typ NYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTD-GroEL_salmonella_typhi_TY2_U01 NYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTD-GroEL_E_coli_K12_AAC77103 NYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTD-GroEL_Yersinia_pestis_NP_99189 SFGYNAYTEEYGDMIAMGILDPTKVTRSALQYAASIAGLMITTECMVTD-GroEL_haemophilus_influenzae_Y NFGYNAGTEQYGDMIEMGILDPTKVTRSALQFAASVAGLMITTECMVTD-GroEL_Legionella_YP_126086 NYGFNAATGEYGDMVEMGILDPTKVTRMALQNAASVASLMLTTECMVAD-GroEL_Burkholderia_multivorans NFGYNAATGEYGDLVEAGVVDPTKVTRTALQNAASIAGLILTTDATVAD-GroEL_Bartonella_henselae_YP_0 TFGYNTATGEFGDLIALGIVDPVKVVRSALQNAASIASLLITTEAMVAE-GroEL_brucella_abortus_ZP_0587 TFGYNTANGEYGDLISLGIVDPVKVVRTALQNAASVAGLLITTEAMIAE-GroEL_Mycobact_tuberc_CAA17397 GHGLNAQTGVYEDLLAAGVADPVKVTRSALQNAASIAGLFLTTEAVVAD-GroEL_Mycobacterium_leprae_TN_ GHGLNAATGEYEDLLKAGVADPVKVTRSALQNAASIAGLFLTTEAVVAD-GroEL_tropheryma_whipplei_NP_7 GHGLDASTGEYVDMLSRGISDPVKVTRSALENAASIAGLFLTTEAVVAE-GroEL_Staph_aureus_MRSA_YP_041 GVGFNAATNEWVNMLEVGIVDPTKVTRSALQHAASVAAMFLTTEAVVAS-GroEL_Listeria_monocytogenes_A GVGFNAANGEWVNMIDAGIVDPTKVTRSALQNASSVAALLLTTEAVVAD-GroEL_streptococcus_pneumoniae GIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVASLILTTEAVVAN-GroEL_mycoplasma_penetrans_NP_ HLGFNAATGKYVDMFQTGIIDPVKVTRIALQNAVSVSSMLLTTEAVIYD-HSP60_Leishmania_Strain_Friedl SFGYNAQTGEYVNMFEAGIIDPMKVVKSAVVNACSVAGMMITTEAAVVEKHSP60_Schistosoma_mansoni_XP_0 NMGYDAQNDAYVDMIEAGIIDPTKVVRTALVDAAGVASLLTTAETVVTD-Hsp60_human_mitochondria_NP_95 EVGYDAMAGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTE-HSP60_Plasmodium_falciparum_XP NIGFNAQEGKYVDMIESGIIDPTKVVKTAISDAASIASLMTTTEVAIVD-chaperonin60_Cryptosporidium_p HFGFDAQTGQFVDMIESGILDPTKVVKSGLRDAASIASLMTTTQVSVFE-Chaperonin60_Entamoeba_histoly SFGYDVRKNVYCDLMKVGIVDPTKVVRNAFNEAISVGSLIATSEALITD-Chaperonin60_Giardia_lamblia_X FYGFDALNGQFVNMEKAGILDATKVVTTALDSALGVSSVLLNTDAVVQP- 650GroEL_orientia_tsutsugamushi_C ----------------------HEEDNNTGNRSGGGVGGGHHGGMGGMDFGroEL_rickettsia_bellii_YP_537 ----------------------EPEDKENPMPMRGGMGG--MGGMGGMDFGroEL5_neoehrlichia_mikurensis --------------------------------------------------GroEL_anaplasma_phagocytophili ----------------------VPSKNDAAGAGAGGMGG--MGGMGGF--GroEL_ehrlichia_deer_AB454077 --------------------------------------------------GroEL_Wolbachia_YP_198181 ----------------------VPNKEENASSSMG-AGG--MGGMNGF--GroEL_Borrelia_garinii_YP_0730 ----------------------IKEE---KNTSGGGGYPMDPGMGMM---GroEL_treponema_pallidum_YP_00 ----------------------IPE----KSSSTPPAPDMG-GMGGMY--GroEL_Leptospira_interrogans_Y ----------------------KPDKDAPNPMAGMGGGGMG-GMGGMM--GroEL_CH60_CHLPN_chlamydophila ----------------------IPEE---KSSSAPAMPSAG--MDY----GroEL3_chlamydia_psittaci_AEG8 ----------------------IPEE---KSSSVPAMPGAG--MDY----GroEL2_salmonella_enterica_typ ----------------------LPK-SDAPDLGAAGGMGGMGGMGGMM--GroEL_salmonella_typhi_TY2_U01 ----------------------LPK-SDAPDLGAAGGMGGMGGMGGMM--GroEL_E_coli_K12_AAC77103 ----------------------LPK-NDAADLGAAGGMGGMGGMGGMM--GroEL_Yersinia_pestis_NP_99189 ----------------------LPR-DDKGADMGAGGMGGMGGMGGMM--GroEL_haemophilus_influenzae_Y ----------------------LPK-DDKADLGAAG-MGGMGGMGGMM--GroEL_Legionella_YP_126086 ----------------------LPKKEEGVGAGDMGGMGGMGGMGGMM--GroEL_Burkholderia_multivorans ----------------------APK-DESAAPAPSPALDY----------GroEL_Bartonella_henselae_YP_0 ----------------------VPKKDTPVPPMPGGGMGGMGGMDF----GroEL_brucella_abortus_ZP_0587 ----------------------LPKKDAAPAGMPGG-MGGMGGMDF----GroEL_Mycobact_tuberc_CAA17397 ----------------------KPEKEKA--SVPG-GGDMG-GMDF----GroEL_Mycobacterium_leprae_TN_ ----------------------KPEKTAA--PASDPTGGMG-GMDF----GroEL_tropheryma_whipplei_NP_7 ----------------------KPEPK----PAPGPADPGA-GMDF----GroEL_Staph_aureus_MRSA_YP_041 ----------------------IPEKNN----DQPNMGGMP-GM------GroEL_Listeria_monocytogenes_A ----------------------KPDENGPAAVPDMGMGGMG-GMM-----GroEL_streptococcus_pneumoniae ----------------------KPEPVAP--APAMDPSMMG-GMM-----GroEL_mycoplasma_penetrans_NP_ ----------------------VKD-DKEDSVPAMPNMGMG-GMM-----HSP60_Leishmania_Strain_Friedl DLLGREKRIEDEGMEDKEKKRSVDKLRKQVNERDAPMPKMAPPMKFDMKGHSP60_Schistosoma_mansoni_XP_0 ----------------------LPKEETGANAAGMGGMGGMGGMGGMM--Hsp60_human_mitochondria_NP_95 ----------------------IPKEEKDPGMGAMGGMG--GGMGGGMF-HSP60_Plasmodium_falciparum_XP ----------------------FKDSKNEESSQHMNSVNSMGDMGGMY--chaperonin60_Cryptosporidium_p ----------------------PSNQSEKNNSSGSNSSESSSSFGSLPGDChaperonin60_Entamoeba_histoly ----------------------EPIKKEIN--------------------Chaperonin60_Giardia_lamblia_X ----------------------IPTDTNLFKNK----------------- GroEL_orientia_tsutsugamushi_C --GroEL_rickettsia_bellii_YP_537 --GroEL5_neoehrlichia_mikurensis --GroEL_anaplasma_phagocytophili --GroEL_ehrlichia_deer_AB454077 --GroEL_Wolbachia_YP_198181 --GroEL_Borrelia_garinii_YP_0730 --GroEL_treponema_pallidum_YP_00 --GroEL_Leptospira_interrogans_Y --GroEL_CH60_CHLPN_chlamydophila --GroEL3_chlamydia_psittaci_AEG8 --GroEL2_salmonella_enterica_typ --GroEL_salmonella_typhi_TY2_U01 --GroEL_E_coli_K12_AAC77103 --GroEL_Yersinia_pestis_NP_99189 --GroEL_haemophilus_influenzae_Y --GroEL_Legionella_YP_126086 --GroEL_Burkholderia_multivorans --GroEL_Bartonella_henselae_YP_0 --GroEL_brucella_abortus_ZP_0587 --GroEL_Mycobact_tuberc_CAA17397 --GroEL_Mycobacterium_leprae_TN_ --GroEL_tropheryma_whipplei_NP_7 --GroEL_Staph_aureus_MRSA_YP_041 --GroEL_Listeria_monocytogenes_A --GroEL_streptococcus_pneumoniae --GroEL_mycoplasma_penetrans_NP_ --HSP60_Leishmania_Strain_Friedl L-HSP60_Schistosoma_mansoni_XP_0 --Hsp60_human_mitochondria_NP_95 --HSP60_Plasmodium_falciparum_XP --chaperonin60_Cryptosporidium_p FYChaperonin60_Entamoeba_histoly --Chaperonin60_Giardia_lamblia_X -- Section 6. Further discussion on HSP60 epitopesAntibodies to certain peptides from human HSP60 (not overlapping the ones preferentially recognized by antibodies in ME samples described here) are also more common in diabetes type 1 compared to controls PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5Ib3J2YXRoPC9BdXRob3I+PFllYXI+MjAwMjwvWWVhcj48

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ADDIN EN.CITE.DATA (17). As shown in Figure 5, several of the chlamydia HSP60 epitopes defined with shorter synthetic peptides PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5Eb21laWthPC9BdXRob3I+PFllYXI+MTk5ODwvWWVhcj48

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ADDIN EN.CITE.DATA (8, 18-20) were also seen in our epitope survey. Compared to controls, secretory IgA reactive with a chlamydia peptide (ATLVGNRIRGGF) was more common in women with infertility ADDIN EN.CITE <EndNote><Cite><Author>Kligman</Author><Year>1996</Year><RecNum>5747</RecNum><DisplayText>(<style face="italic">20</style>)</DisplayText><record><rec-number>5747</rec-number><foreign-keys><key app="EN" db-id="f29rxsde60p9fsev5d9v9relserrtavztsvz">5747</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Kligman, I.</author><author>Grifo, J. A.</author><author>Witkin, S. S.</author></authors></contributors><auth-address>Center for Reproductive Endocrinology and Infertility, Cornell University Medical College, New York, NY 10021, USA.</auth-address><titles><title>Expression of the 60 kDa heat shock protein in peritoneal fluids from women with endometriosis: implications for endometriosis-associated infertility</title><secondary-title>Human reproduction</secondary-title><alt-title>Hum Reprod</alt-title></titles><periodical><full-title>Human reproduction</full-title><abbr-1>Hum Reprod</abbr-1></periodical><alt-periodical><full-title>Human reproduction</full-title><abbr-1>Hum Reprod</abbr-1></alt-periodical><pages>2736-8</pages><volume>11</volume><number>12</number><edition>1996/12/01</edition><keywords><keyword>Adult</keyword><keyword>Antibodies, Bacterial/analysis</keyword><keyword>Ascitic Fluid/*chemistry</keyword><keyword>Chaperonin 60/*analysis/immunology</keyword><keyword>Chlamydia trachomatis/immunology</keyword><keyword>Endometriosis/complications/*metabolism</keyword><keyword>Female</keyword><keyword>Humans</keyword><keyword>Infertility, Female/*etiology/immunology</keyword><keyword>Interferon-gamma/analysis</keyword></keywords><dates><year>1996</year><pub-dates><date>Dec</date></pub-dates></dates><isbn>0268-1161 (Print)&#xD;0268-1161 (Linking)</isbn><accession-num>9021381</accession-num><urls><related-urls><url>;(20) and was more commonly recognized by IgG from women with pelvic inflammatory disease PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5Eb21laWthPC9BdXRob3I+PFllYXI+MTk5ODwvWWVhcj48

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ADDIN EN.CITE.DATA (8, 19) (marked in the alignment of Supplementary Figure SF2). Our longer peptides detected some, but not all, of these epitopes, using BD and ME patient samples. It remains to investigate if hidden in these reactions lie diagnostically useful epitopes, and whether there exist disease-specific Chlamydia pneumoniae HSP60 antibody patterns with our set of peptides, aside from the selective ME reactions reported here. Knowing that HSP60 is a highly conserved and cross-reactive protein, the ability of some Chlamydia pneumoniae, and Mycoplasma penetrans HSP60 peptides to selectively detect IgM antibodies in blood samples from ME patients cannot be taken as definite evidence for Chlamydia or Mycoplasma as etiologic agents in ME. The matter requires an investigation with more HSP60 antigens than was possible in this report.Other disease associations of antibodies reacting with HSP60 peptides, and their crossreactivities.In the mycobacterially induced adjuvant arthritis of rats, the arthritis starts a T cell response to a centrally placed HSP60 epitope which subsides when a response to the C terminal portion of HSP65 occurs PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5Nb3VkZ2lsPC9BdXRob3I+PFllYXI+MjAwODwvWWVhcj48

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ADDIN EN.CITE.DATA (21). The humoral anti-HSP60 response appears to protect against the arthritis PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5LaW08L0F1dGhvcj48WWVhcj4yMDA2PC9ZZWFyPjxSZWNO

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ADDIN EN.CITE.DATA (22). The antibody response initially involves several epitopes, but later narrows down to fewer epitopes. This is reminiscent of the epitope differences in the IgM and IgG tests seen in this work, where IgM epitopes were more numerous than the IgG ones. The Porphyromonas gingivalis peptide 19 (TLVVNRLRGSLKICAVKAPG) was frequently antigenic in patients suffering from cardiovascular disease PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5XeXNvY2tpPC9BdXRob3I+PFllYXI+MjAwMjwvWWVhcj48

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ADDIN EN.CITE.DATA (5, 23). It contains nine (underlined) of the 16 consensus amino acids critical for detection of antibodies in some ME patients defined here. It contains a part of apical helix I (italics). The possibility that peptide p19 and the G20/G20c homologs share some antigenicity should be tested experimentally. Antibodies to certain peptides from human HSP60 are also more common in T1D compared to controls PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5Ib3J2YXRoPC9BdXRob3I+PFllYXI+MjAwMjwvWWVhcj48

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ADDIN EN.CITE.DATA (17). There exists a correlation between enterovirus infection and T1D, see e.g. PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5FbGZhaXRvdXJpPC9BdXRob3I+PFllYXI+MjAwNzwvWWVh

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ADDIN EN.CITE.DATA (26-29), postinfectious encephalomyelitis, including the Guillain-Barré syndrome ADDIN EN.CITE <EndNote><Cite><Author>Wingerchuk</Author><Year>2003</Year><RecNum>5604</RecNum><DisplayText>(<style face="italic">30</style>)</DisplayText><record><rec-number>5604</rec-number><foreign-keys><key app="EN" db-id="f29rxsde60p9fsev5d9v9relserrtavztsvz">5604</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Wingerchuk, D. M.</author></authors></contributors><auth-address>Department of Neurology, Mayo Clinic, 13400 East Shea Boulevard, Scottsdale, AZ 85259, USA. wingerchuk.dean@mayo.edu</auth-address><titles><title>Postinfectious encephalomyelitis</title><secondary-title>Current neurology and neuroscience reports</secondary-title><alt-title>Curr Neurol Neurosci Rep</alt-title></titles><periodical><full-title>Current neurology and neuroscience reports</full-title><abbr-1>Curr Neurol Neurosci Rep</abbr-1></periodical><alt-periodical><full-title>Current neurology and neuroscience reports</full-title><abbr-1>Curr Neurol Neurosci Rep</abbr-1></alt-periodical><pages>256-64</pages><volume>3</volume><number>3</number><edition>2003/04/15</edition><keywords><keyword>Adolescent</keyword><keyword>Adult</keyword><keyword>Child</keyword><keyword>Child, Preschool</keyword><keyword>Diagnosis, Differential</keyword><keyword>Encephalomyelitis, Acute Disseminated/classification/*diagnosis/therapy/virology</keyword><keyword>Humans</keyword><keyword>Magnetic Resonance Imaging</keyword><keyword>Middle Aged</keyword><keyword>Multiple Sclerosis/diagnosis</keyword><keyword>Recurrence</keyword><keyword>Virus Diseases/complications</keyword></keywords><dates><year>2003</year><pub-dates><date>May</date></pub-dates></dates><isbn>1528-4042 (Print)&#xD;1528-4042 (Linking)</isbn><accession-num>12691631</accession-num><work-type>Review</work-type><urls><related-urls><url>;(30), and pediatric neuropsychiatric disorder with tics and Sydenham?s chorea after streptococcal infection PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5LdXJsYW48L0F1dGhvcj48WWVhcj4yMDA0PC9ZZWFyPjxS

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ADDIN EN.CITE.DATA (31-33) are now known. Other diseases with autoimmunity to specific brain tissues are multiple sclerosis ADDIN EN.CITE <EndNote><Cite><Author>Comabella</Author><Year>2012</Year><RecNum>5736</RecNum><DisplayText>(<style face="italic">34</style>)</DisplayText><record><rec-number>5736</rec-number><foreign-keys><key app="EN" db-id="f29rxsde60p9fsev5d9v9relserrtavztsvz">5736</key></foreign-keys><ref-type name="Journal Article">17</ref-type><contributors><authors><author>Comabella, M.</author><author>Khoury, S. J.</author></authors></contributors><auth-address>Centre d&apos;Esclerosi Multiple de Catalunya, CEM-Cat, Unitat de Neuroimmunologia Clinica, Hospital Universitari Vall d&apos;Hebron (HUVH), Barcelona, Spain. mcomabel@ir.</auth-address><titles><title>Immunopathogenesis of multiple sclerosis</title><secondary-title>Clinical immunology</secondary-title><alt-title>Clin Immunol</alt-title></titles><periodical><full-title>Clinical immunology</full-title><abbr-1>Clin Immunol</abbr-1></periodical><alt-periodical><full-title>Clinical immunology</full-title><abbr-1>Clin Immunol</abbr-1></alt-periodical><pages>2-8</pages><volume>142</volume><number>1</number><edition>2011/04/05</edition><keywords><keyword>Humans</keyword><keyword>Multiple Sclerosis/*immunology</keyword></keywords><dates><year>2012</year><pub-dates><date>Jan</date></pub-dates></dates><isbn>1521-7035 (Electronic)&#xD;1521-6616 (Linking)</isbn><accession-num>21458377</accession-num><work-type>Review</work-type><urls><related-urls><url>;(34) and anti-NMDA receptor encephalitis PEVuZE5vdGU+PENpdGU+PEF1dGhvcj5QaWVobDwvQXV0aG9yPjxZZWFyPjIwMTA8L1llYXI+PFJl

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ADDIN EN.CITE.DATA (38-48).Section 9. Other supplementary informationSupplementary Figure SF6. Needle plots of Chlamydia pneumoniae HSP60 peptides with significant reactivity and selectivity for ME. Results with peptides coupled with short and long spacer, IgG and IgM are shown. Abscissas: Patient categories as detailed in the legend of Figure 1. Ordinates: MFI.Supplementary figure SF7. Epitope scanning with overlapping HSP60 peptides from Mycoplasma penetrans.Supplementary figure SF8.Needle plots of antigenicity of the most discriminatory Mycoplasma penetrans peptides separated according to Test set patient category and BD status (cf Figure SF3). Ordinates: MFI. Abscissae: Categories. A. IgG data. B. IgM data. The pattern for IgG reactivity of the Mycoplasma HSP60 peptides was somewhat different from that of the Chlamydia HSP60 peptides, with D18 (EALTTLVVNKMRGVFNVVAVKAPEFGDKRK, Supplementary Table ST4) being largely non-reactive. However, the separately synthesized Mycoplasma penetrans G20c homolog (LTTLVVNKMRGVFNVVAVKAPEFGDKRKQV, Supplementary Table ST3) reacted with a strong ME sample preference. The D18 peptide contained the whole apical helix I sequence (underlined) and the G20c homolog contained most of it.Supplementary table ST8. Panel of antigen candidates.Short nameAntigen typeOrigin of HSP60P5G20c homolog peptideBorrelia gariiniP7“Tropheryma whippleiP11“Escherichia coliP12“Chlamydia pneumoniaeP13“Staphylococcus aureusP15“Mycoplasma penetransP16“Leishmania majorP17“Schistosoma mansoniiP18“Plasmodium falciparumP19“Leptospira interrogansP20“Listeria monocytogenesP22“Treponema pallidumP24“Cryptosporidium parvumP25“Entamoeba histolyticaD9Peptide from N terminal halfMycoplasma penetransD25Peptide from C terminal halfMycoplasma penetransC18Peptide overlapping G20c, short spacerChlamydia pneumoniaeC25Peptide from C terminal half, short spacerChlamydia pneumoniaeC29Peptide from C terminal half, short spacerChlamydia pneumoniaeE18Peptide overlapping g20c, long spacerChlamydia pneumoniaeE25Peptide from C terminal half, long spacer Chlamydia pneumoniaeG20Peptide overlapping g20cHomo sapiensG20cg20c homolog peptideHomo sapiensHuman HSP60Recombinant human HSP60Homo sapiensE coli HSP60Recombinant E coli HSP60 (“GroEL”)Escherichia coliResults from the evaluation stageFigure SF9. Overview of the results from the evaluation stageFigure SF9 depicts the result of the IgM test of the selected antigens with the Evaluation sample set. Blood donors from 2010 (n=91) gave lower values than the other blood donor groups (BD2005, n=50, and BD2013, n=161) for all antigens. ME (n=61), MS (n=20) and SLE (n=48) samples were included. The differences between ME and BD observed in the Test set, where a different set of blood donors from 2010 was used, could not be reproduced in the Evaluation set, with the exception of the P12 (Chlamydia pneumoniae G20c homolog) antigen. Thus, the date of blood donation influenced the Test set results.We wanted to test the hypothesis that the IgM reactivity of the p12 peptide simply reflected the presence of IgM reactive with whole Chlamydia pneumoniae antigen. As seen in supplementary figure SF10 A the reactivity of this antigen was not higher in ME patients than in MS and SLE patients and blood donors. The whole antigen relative MFI values did not correlate with p12 relative MFI. Supplementary figure SF10 B shows that there is no evidence for a correlation of these two serological variables. Figure SF10. Results with elementary body Chlamydia pneumoniae antigen. A. Needle plot of MFI for the categories ME, MS, SLE and BD. B. Scatter plot of MFI of Chlamydia antigen versus that of peptide p12 (Chlamydia homolog of G20c). Supplementary figure SF11. ROC curve for discrimination of ME from BD by p12 and IgM in the Evaluation set. Two breakpoints were calculated, either with a relative MFI of 1, optimizing for sensitivity, or a relative MFI of 3, optimizing for positive predictive value. ADDIN EN.REFLIST References1.L. Chen, P. B. Sigler, The crystal structure of a GroEL/peptide complex: plasticity as a basis for substrate diversity. Cell 99, 757 (Dec 23, 1999).2.H. Perschinka et al., Cross-reactive B-cell epitopes of microbial and human heat shock protein 60/65 in atherosclerosis. Arterioscler Thromb Vasc Biol 23, 1060 (Jun 1, 2003).3.E. Jeong, J. Y. Lee, S. J. Kim, J. Choi, Predominant immunoreactivity of Porphyromonas gingivalis heat shock protein in autoimmune diseases. J Periodontal Res 47, 811 (Dec, 2012).4.T. Okada et al., Antibodies against heat shock protein 60 derived from Helicobacter pylori: diagnostic implications in cardiovascular disease. Journal of autoimmunity 29, 106 (Sep-Nov, 2007).5.J. Wysocki et al., Human heat shock protein 60 (409-424) fragment is recognized by serum antibodies of patients with acute coronary syndromes. Cardiovasc Pathol 11, 238 (Jul-Aug, 2002).6.J. J. Hansen et al., Hereditary spastic paraplegia SPG13 is associated with a mutation in the gene encoding the mitochondrial chaperonin Hsp60. Am J Hum Genet 70, 1328 (May, 2002).7.T. Harkonen et al., Picornavirus proteins share antigenic determinants with heat shock proteins 60/65. J Med Virol 62, 383 (Nov, 2000).8.Y. Yi, G. Zhong, R. C. Brunham, Continuous B-cell epitopes in Chlamydia trachomatis heat shock protein 60. Infect Immun 61, 1117 (Mar, 1993).9.Y. Xue, M. L. O'Mara, P. P. Surawski, M. Trau, A. E. Mark, Effect of poly(ethylene glycol) (PEG) spacers on the conformational properties of small peptides: a molecular dynamics study. Langmuir 27, 296 (Jan 4, 2011).10.J. Blomberg et al., No evidence for xenotropic murine leukemia-related virus infection in Sweden using internally controlled multiepitope suspension array serology. Clin Vaccine Immunol 19, 1399 (Sep, 2012).11.A. Sheikholvaezin, F. Blomberg, C. Ohrmalm, A. Sjosten, J. Blomberg, Rational recombinant XMRV antigen preparation and bead coupling for multiplex serology in a suspension array. Protein Expr Purif 80, 176 (Dec, 2011).12.B. Akerstrom, T. Brodin, K. Reis, L. Bjorck, Protein G: a powerful tool for binding and detection of monoclonal and polyclonal antibodies. J Immunol 135, 2589 (Oct, 1985).13.L. Bjorck, J. Blomberg, Streptococcal protein G: a sensitive tool for detection of antibodies to human immunodeficiency virus proteins in Western blot analysis. Eur J Clin Microbiol 6, 428 (Aug, 1987).14.J. Michaelsson et al., A signal peptide derived from hsp60 binds HLA-E and interferes with CD94/NKG2A recognition. J Exp Med 196, 1403 (Dec 2, 2002).15.R. Lachumanan, S. Devi, Y. M. Cheong, S. J. Rodda, T. Pang, Epitope mapping of the Sta58 major outer membrane protein of Rickettsia tsutsugamushi. Infect Immun 61, 4527 (Oct, 1993).16.K. Braig et al., The crystal structure of the bacterial chaperonin GroEL at 2.8 A. Nature 371, 578 (Oct 13, 1994).17.L. Horvath et al., Antibodies against different epitopes of heat-shock protein 60 in children with type 1 diabetes mellitus. Immunol Lett 80, 155 (Mar 1, 2002).18.M. Domeika, K. Domeika, J. Paavonen, P. A. Mardh, S. S. Witkin, Humoral immune response to conserved epitopes of Chlamydia trachomatis and human 60-kDa heat-shock protein in women with pelvic inflammatory disease. J Infect Dis 177, 714 (Mar, 1998).19.I. Sziller et al., Serological responses of patients with ectopic pregnancy to epitopes of the Chlamydia trachomatis 60 kDa heat shock protein. Hum Reprod 13, 1088 (Apr, 1998).20.I. Kligman, J. A. Grifo, S. S. Witkin, Expression of the 60 kDa heat shock protein in peritoneal fluids from women with endometriosis: implications for endometriosis-associated infertility. Hum Reprod 11, 2736 (Dec, 1996).21.K. D. Moudgil, M. Durai, Regulation of autoimmune arthritis by self-heat-shock proteins. Trends Immunol 29, 412 (Sep, 2008).22.H. R. Kim, E. Y. Kim, J. Cerny, K. D. Moudgil, Antibody responses to mycobacterial and self heat shock protein 65 in autoimmune arthritis: epitope specificity and implication in pathogenesis. J Immunol 177, 6634 (Nov 15, 2006).23.H. Perschinka et al., Identification of atherosclerosis-associated conformational heat shock protein 60 epitopes by phage display and structural alignment. Atherosclerosis 194, 79 (Sep, 2007).24.A. Elfaitouri et al., Recent enterovirus infection in type 1 diabetes: evidence with a novel IgM method. J Med Virol 79, 1861 (Dec, 2007).25.T. Harkonen et al., Enterovirus infection may induce humoral immune response reacting with islet cell autoantigens in humans. J Med Virol 69, 426 (Mar, 2003).26.B. R. Kornum, J. Faraco, E. Mignot, Narcolepsy with hypocretin/orexin deficiency, infections and autoimmunity of the brain. Curr Opin Neurobiol 21, 897 (Dec, 2011).27.B. R. Kornum et al., Common variants in P2RY11 are associated with narcolepsy. Nat Genet 43, 66 (Jan, 2011).28.J. Hallmayer et al., Narcolepsy is strongly associated with the T-cell receptor alpha locus. Nat Genet 41, 708 (Jun, 2009).29.A. Fontana et al., Narcolepsy: autoimmunity, effector T cell activation due to infection, or T cell independent, major histocompatibility complex class II induced neuronal loss? Brain 133, 1300 (May, 2010).30.D. M. Wingerchuk, Postinfectious encephalomyelitis. Curr Neurol Neurosci Rep 3, 256 (May, 2003).31.R. Kurlan, E. L. Kaplan, The pediatric autoimmune neuropsychiatric disorders associated with streptococcal infection (PANDAS) etiology for tics and obsessive-compulsive symptoms: hypothesis or entity? Practical considerations for the clinician. Pediatrics 113, 883 (Apr, 2004).32.T. K. Murphy, R. Kurlan, J. Leckman, The immunobiology of Tourette's disorder, pediatric autoimmune neuropsychiatric disorders associated with Streptococcus, and related disorders: a way forward. J Child Adolesc Psychopharmacol 20, 317 (Aug, 2010).33.J. F. Baizabal-Carvallo, J. Jankovic, Movement disorders in autoimmune diseases. Mov Disord 27, 935 (Jul, 2012).34.M. Comabella, S. J. Khoury, Immunopathogenesis of multiple sclerosis. Clin Immunol 142, 2 (Jan, 2012).35.F. Piehl, K. Winnerback, [Newly discovered autoimmune encephalitis wrongly mistaken for mental disease. Anti-NMDA receptor encephalitis puts demands on correct differential diagnosis]. Lakartidningen 107, 589 (Mar 3-9, 2010).36.M. J. Mansilla, X. Montalban, C. Espejo, Heat shock protein 70: roles in multiple sclerosis. Mol Med 18, 1018 (2012).37.F. J. Quintana et al., Antigen microarrays identify unique serum autoantibody signatures in clinical and pathologic subtypes of multiple sclerosis. Proc Natl Acad Sci U S A 105, 18889 (Dec 2, 2008).38.G. L. Nicolson, R. Gan, J. Haier, Multiple co-infections (Mycoplasma, Chlamydia, human herpes virus-6) in blood of chronic fatigue syndrome patients: association with signs and symptoms. Apmis 111, 557 (May, 2003).39.S. D. Vernon, S. K. Shukla, W. C. Reeves, Absence of Mycoplasma species DNA in chronic fatigue syndrome. J Med Microbiol 52, 1027 (Nov, 2003).40.G. K. Endresen, Mycoplasma blood infection in chronic fatigue and fibromyalgia syndromes. Rheumatol Int 23, 211 (Sep, 2003).41.J. Nijs, G. L. Nicolson, P. De Becker, D. Coomans, K. De Meirleir, High prevalence of Mycoplasma infections among European chronic fatigue syndrome patients. Examination of four Mycoplasma species in blood of chronic fatigue syndrome patients. FEMS Immunol Med Microbiol 34, 209 (Nov 15, 2002).42.M. Nasralla, J. Haier, G. L. Nicolson, Multiple mycoplasmal infections detected in blood of patients with chronic fatigue syndrome and/or fibromyalgia syndrome. Eur J Clin Microbiol Infect Dis 18, 859 (Dec, 1999).43.A. Vojdani et al., Detection of Mycoplasma genus and Mycoplasma fermentans by PCR in patients with Chronic Fatigue Syndrome. FEMS Immunol Med Microbiol 22, 355 (Dec, 1998).44.A. L. Komaroff, D. S. Bell, P. R. Cheney, S. C. Lo, Absence of antibody to Mycoplasma fermentans in patients with chronic fatigue syndrome. Clin Infect Dis 17, 1074 (Dec, 1993).45.J. K. Chia, L. Y. Chia, Chronic Chlamydia pneumoniae infection: a treatable cause of chronic fatigue syndrome. Clin Infect Dis 29, 452 (Aug, 1999).46.L. D. Devanur, J. R. Kerr, Chronic fatigue syndrome. J Clin Virol 37, 139 (Nov, 2006).47.J. E. Greenlee, J. W. Rose, Controversies in neurological infectious diseases. Semin Neurol 20, 375 (2000).48.A. L. Komaroff, S. P. Wang, J. Lee, J. T. Grayston, No association of chronic Chlamydia pneumoniae infection with chronic fatigue syndrome. J Infect Dis 165, 184 (Jan, 1992). ................
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