Lab IV: Recombinant DNA Analysis
5. Add 26 (L of ddH2O and 4 (L of EB to each of your tubes 1-4. Add 5 (L of ddH2O and 1 (L of EB to the shared tubes A and B. 6. Add 10 (L of pUC-Kan to tubes 1 and 3. Add 10 (L of pBR322 to tubes 2 and 4. Add only 4 (L of pUC-Kan to tube A and 4 (L of pBR322 to tube B. Mix by “finger vortex” (ask for demo if you are unclear on what this is ... ................
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