Lab IV: Recombinant DNA Analysis
C. Purify the plasmid DNA. 7. Centrifuge for 10 minutes at max speed. A white pellet will form. 8. Apply the supernatant to a plasmid prep column by decanting, or using a pipet. Your plasmid DNA will be retained on the silica-gel membrane inside due to the high salt conditions of the supernatant. ................
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