University of Manchester



Whole genome microarray data supports the importance of chronic wound debridement prior to application of dermal skin substitutes

Mohammed Ashrafi MB ChB1, Anil Sebastian PhD1, Barbara Shih1 PhD, Nicholas Greaves1 PhD, Teresa Alonso-Rasgado PhD2, Mohamed Baguneid MD3, Ardeshir Bayat MB BS PhD1*

1Plastic and Reconstructive Surgery Research, Centre for Dermatological Research, Institute of Inflammation and Repair, University of Manchester, UK

2Bioengineering Group, School of Materials, University of Manchester, UK

3University Hospital South Manchester NHS Foundation Trust, Wythenshawe Hospital, Manchester, UK

*Corresponding author at: Dr Ardeshir Bayat, Plastic and Reconstructive Surgery Research, Centre for Dermatological Research, Institute of Inflammation and Repair, University of Manchester, Oxford Road, Manchester, M13 9PT, UK Tel.: +44 161 306 0607. E-mail address: ardeshir.bayat@manchester.ac.uk

Running title: Whole genome microarray data supports the importance of chronic wound debridement prior to application of dermal skin substitutes

Keywords: microarray; dermal skin substitutes; chronic wounds; decellularised dermis

ABSTRACT

Clinical consensus is that debridement is necessary for successful application of dermal skin substitutes (DSS) to chronic wounds. The aim was to identify commonly expressed genes associated with wound healing in untreated acute wounds and chronic wounds treated with wound debridement followed by DSS. Cutaneous biopsies were taken at two time points from untreated acute and chronic wounds and from chronic wounds treated with DSS following debridement. Microarray analysis identified significant differences (p1.01 were identified. For chronic wound normalised data genes with fold change ratio of 1.01) and those same genes subsequently down-regulated in chronic wounds (fold change ratio ................
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