Week - Los Angeles Mission College



MICROBIOLOGY 20: General Microbiology Lecture Section 17288 Los Angeles Mission CollegeFall, 2020Lecture: Tue & Thu 5:15-6:40 PM (Online by Zoom)Laboratory Sections: Section 17289: Tuesday 6:50-10:00 PM (Online)Section 17420: Thursday 6:50 AM-10:00 PM (Online)Angela Echeverri, Ph.D.Message through Canvas or e-mail: echeveac@laccd.edu Office hours: Held online via Zoom Tu & Th 5:00-5:15 pm or by appointmentPrerequisites: Successful completion of the following courses: Biology 3, AND Chemistry 51, 65 or 101 (or the equivalent) Hours: Lecture – 3 Carnegie hours/wk, Laboratory – 3 Carnegie hours/week; 4 semester units. This course is UC:CSU Transferable. Articulation: Introductory Microbiology with laboratory (BIOL 215 & 215L) at CSU Northridge. Visit to view all articulation agreements. Course Description - This course examines microbiological principles related to the morphology, metabolism and genetics of bacteria and distinguishing characteristics of viruses, protozoa, fungi, and multicellular parasites, with applications to human disease. It is intended primarily for allied health majors with 3 hours of lab each week. Student Learning OutcomesStudents will successfully perform and interpret a Gram stain, compare and contrast the structures of Gram–negative and Gram-positive cell walls, and address why each stains differently with a Gram stain.Students will demonstrate understanding of key concepts in the course by designing a brochure or poster for an infectious disease or immune system disorder.(3) Students will identify an unknown bacterium using laboratory skills learned in the course.TEACHING PHILOSOPHY: Microbiology is a fascinating discipline that is rapidly changing and always in the news, especially during the current COVID-19 pandemic. The field encompasses a wide range of specialties including bacteriology, parasitology, virology, epidemiology, genetics, cell & molecular biology, and immunology. Since most of you are allied health majors, we will emphasize infectious and immune system diseases, as well as how to prevent and/or treat them. In this class you will also learn how to use the microscope, aseptic technique, staining methods, and metabolic test results. In order to get the most out of your learning experience, study on a regular basis, plan ahead, be prepared for class, ask questions, participate fully in the lab experiments, monitor your progress online regularly, and seek help early when you need it. I encourage you to take full advantage of the many resources available to help you develop effective study habits and succeed in Microbiology 20 at Los Angeles Mission College.Required Books and MaterialsMICROBIOLOGY. Nina Parker et al, 1st ed. 2018 Rice University. Open Stax/ASM Press. Textbook: 978-1-947172-23-4 (PDF version); ISBN 978-1-938168-14-47 (print version). Available for free download at <; Printed copies are also available in the LAMC BookstoreMICROBIOLOGY Laboratory Theory & Application (LAMC Custom 10th edition). Michael Leboffe & Burton Pierce, 2016. Morton Publishing Company (ISBN 978-1-61731-701-9). MATERIALS: Bound notebook for lab (graph ruled), Microbiology kit and microscope (to be distributed), Sharpie pen, colored pencil set, 8 Scantron 882-E forms, box of gloves (optional), and digital camera (optional).Course GradeThere will be 1000 possible points for the entire course as shown below:LECTURE PORTION (650 Points)LAB PORTION (350 Points)3 Lecture Midterm Exams 300 pointsMorphological UnknownBiochemical Unknown30 points70 pointsLecture Final Exam100 pointsLab Quizzes 70 pointsOnline Lecture Quizzes 160 pointsLab Practical Final Exam100 pointsOnline Discussions40 pointsWritten Assignment50 pointsLabster Simulations & Participation60 pointsLab Notebook20 points Lecture ASSIGNMENTS: Exams & Quizzes: Exams and quizzes will consist of multiple choice, matching, true/false, short answer, and essay questions. Exam multiple-choice and matching questions are to be answered online. No make-up exams will be given. The first missed exam score will be replaced by the average of the highest and lowest midterm exams taken during the semester. Lecture quizzes will be administered online using the Canvas Web site; most quizzes will open once each chapter is covered and close 3 days later at midnight. You are responsible for taking the quizzes by the deadline. Written Assignment: Students will prepare a tri-fold brochure on an infectious or immune system disease. Guidelines for the Disease Brochure will be detailed in a separate handout. Online Assignments: You will have several online assignments to be completed on Canvas. LabORATORY ASSIGNMENTS Participation: Students are expected to read each lab exercise BEFORE class. Lab participation scores will be based on preparation, attendance, completion of labs, lab safety, cleanup, teamwork, microscope care and usage, appropriate use of equipment and supplies, as well as participation in class and online activities. You will be assigned a microbiology kit and microscope for the semester, which will be distributed by the LAMC Bookstore. You are responsible for the proper use and maintenance of the microscope. You will be allowed to miss one laboratory without penalty. Ten points will be deducted for each unexcused absence from the laboratory. Additional absences will impact your grade. Unknown Reports: You will turn in two unknown reports, a morphological unknown and a biochemical unknown. The forms for both reports are posted on Canvas and my Web site.Labster Simulations: You will need to complete several Labster simulations in Canvas. Lab Quizzes: Several lab quizzes will be administered online during the semester. Lab Notebook: Students will keep a log of experimental procedures and results in a lab notebook, which will be checked and graded by the instructor. See lab schedule for labs to be recorded.Lab Exam: For the laboratory final exam you will be allowed to use your lab notebook. Therefore it is important that you keep accurate and complete records of your lab results. Extra Credit Disease Presentations: You have the option of giving a presentation during lab on your disease topic for 20 extra credit points. Course GradeYou can earn a total of 1000 points for the entire course as shown below:Grading Scale:Out of a total of 1000 points:900+ points (90-100%)A780-899 points (78-89%)B650-779 points (65-77%)C550-649 points (55-64%)D0-549 points (below 55%)FATTENDANCE POLICYAttendance is required and roll will be taken. You are responsible for any information, date changes, etc., presented in class, whether or not you are present. Exchange contact information with at least two other classmates in case you are late or cannot attend class. Students missing more than 2 consecutive classes may be dropped.Students who are adding must complete the process by Sunday, September 13th. If you stop attending or wish to drop this class you must drop the course yourself online or in person by the deadline. Failure to do so may result in a grade of “F” in the class. Students withdrawing from the course must do so by the following dates:Sunday, September 13th: Last day to drop 16-week classes without incurring fees and without a “W” on your transcript.Sunday, September 22nd: Last day to drop class with a “W.” A letter grade is given after this date.Note: State policy limits students to 3 attempts per course.? Receiving a grade or "W" for a course counts as an attempt, regardless of when the course was taken.? Withdrawal by 9/13/2020 will avoid a "W" and will not count as an attempt.Recommendations for SuccessMicrobiology 20 is a demanding class covering a lot of information. Don’t procrastinate! On average, full-time college students study 15 hours a week. To make the most of your study time:Get organized: Staying organized is key. Make sure you have a notebook/binder for each class and organize your notes, handouts, graded exams, reports, and quizzes. When you prepare to study, have all the materials (e.g.: syllabus, pens, paper, calculator, books) that you will need for your study session. Use a planner to keep track of due dates, exams, quizzes, and projects. Keep all of your records until you receive your final course grade. Check Canvas daily: Use to take quizzes, check your scores, read announcements, download handouts, and communicate with your instructor and classmates. Pick your study time and place: Keep up with the material by studying on a regular basis. Experts advise students to study in chunks of shorter time and to avoid marathon study sessions or all-nighters. Set a study schedule and stick to it. Find a comfortable place to study with good lighting and few distractions. Every campus has great places to study. Your room or home may not be the best place to study since there may a TV, bed, roommate, or other distractions to lure you away from your work. Avoid multi-tasking and being distracted by texts or phone calls during study time. After a productive study session reward yourself with a break, but get back to work until you have accomplished your mission. Take good notes and review them correctly: Class notes are a crucial part of studying in college. Record the main points and concentrate on key words, leaving plenty of blank space to fill in later. Make note of any questions you have. Keep your notes neat and in order. Each time you study, read you textbook, spend time adding details, learn key terms, and organize your notes. Use associations, acronyms, and flash cards to help you remember information. Review each topic at least three times; this is the secret to long-term memory.Read effectively: Read actively, searching for the main points. Learn to scan for headings, introductions, keywords, and summaries. Take notes, write summaries, and test yourself. Get help early and often: Attend free tutoring and workshop sessions offered by the SSC (see resources on page 5). Form study groups with your classmates and come to office hours.Participate: In college as in life, 90% of success is showing up. Come to class prepared. Participate in lecture and lab and ask questions if you have any. ***Prepared students do well in this class***SPECIAL ACCOMMODATIONSIf you require special accommodations for a disability, religious holiday, or any other reason, please inform your instructor within the first week of the course and we will accommodate you if at all possible. For accommodations due to disability, consult with the Disabled Student Programs and Services office (see resources on page 5), and they will generate an accommodation letter with their recommendations.ONLINE RESOURCES FOR MICROBIOLOGY 20 STUDENTSYour instructor’s LAMC Web site: Web site: Announcements, quizzes, handouts, Zoom class recordings, and other important information will be posted on this site. You can use the Canvas site to take your quizzes, monitor grades, and communicate with your instructor and each other, as well as ask/answer questions about lecture, laboratory, and assignments. If you already have used a Canvass account, sign in using your userid and password. If you have never used a Canvas account before you will need to set up your account. Detailed instructions, tutorials, and support for online students can be viewed at Textbook Web site: Life Sciences Department Web site: District Web site: To add/drop courses or view your grades.sTUDENT RESOURCES FOR ONLINE/REMOTE INSTRUCTIONLACCD Student Distance Learning ResourcesEnroll Here to access these resourcesCanvas Student?Self-Orientation to CanvasEnroll Here to access this resourceCOLLEGE RESOURCES FOR MICROBIOLOGY 20 STUDENTSAdmissions and Records: Register for classes, request transcripts, file petitions for graduation, and drop classes at this office. For information call (818) 833-3322 or visit: : For hours of operation, book availability, buybacks, and other information call (818) 364-7767 or 7768 or visit Counseling Department: Call (818) 364-7655 or visit Students Programs and Services (DSP&S): For appointments, eligibility, services, and information call (818) 364-7732 or visit Extended Opportunity Programs and Services (EOPS): For appointments, eligibility and information call (818) 364-7645 or visit Financial Aid: For information call (818) 364-7648 or visit (TAP) Program: Call (818) 364-7888 or visit Library: For information contact (818) 364-7106 or visit Tutoring Services in Learning Center: (818) 364-7754 or visit lamission.edu/learningcenter/ STEM Office: For information on resources and services for STEM (Science, Technology, Engineering, and Math) students visit the STEM Center in Room CMS 8 or OF STRESS AND MENTAL HEALTH: As a student, not only do you have the pressure of succeeding in school, you may also be contending with the COVID-19 pandemic, work, financial issues, relationships, managing time effectively, getting enough sleep, etc. The staff and faculty of Los Angeles Mission College are here to provide support that will help you to be successful in your academic pursuits. You can learn more about the broad range of confidential student services offered, including counseling and mental health services, by visiting the Student Health Center (SHC) webpage at: ? or calling: 818 362-6182. I also encourage you to save the National Suicide Prevention Lifeline number (1-800-273-8255) into your cell phone in case you or someone you know is in distress and needs someone with whom to talk.TENTATIVE LECTURE SCHEDULE-FALL 2020Dates are subject to change as neededWeekDateTuesday (Lecture)DateThursday (Lecture)19/1Introduction to Microbiology 20Chapter 1: An Invisible World9/3Chapter 1: An Invisible WorldQuiz 1: Chapter 1 (online)29/8Chapter 7: Chemistry Review9/10Chapter 7: Chemistry ReviewQuiz 2: Chapter 7 (online)39/15Chapter 3: The Cell9/17Chapter 3: The CellQuiz 3: Chapter 3 (online)49/22Chapter 2: How We See the Invisible World (Microscopy)9/24Chapter 2: How We See the Invisible World (Microscopy)Quiz 4: Chapter 2 (online)59/29Chapter 4: Prokaryotic DiversityEXAM 1: Chapters 1-3 & 710/1Chapter 5: The EukaryotesQuiz 5: Chapter 4 (online)610/6Chapter 5: The Eukaryotes of Microbiology10/8Chapter 9: Microbial Growth Quiz 6 & 7: Chapters 5 & 9 (online)710/13Chapter 9: Microbial Growth Ch 13: Control of Microbial Growth10/15Ch 13: Control of Microbial Growth Quiz 8: Chapter 9 (online)810/20Chapter 14: Antimicrobial Drugs10/22Chapter 14: Antimicrobial DrugsQuiz 9: Chapter 13 (online)910/27Chapter 6: Acellular AgentsEXAM 2: Chapter 4, 5, 9, 13 & 1410/29Ch 6: Acellular Agents & VaccinesQuiz 10: Chapter 6 & Vaccines(online)1011/3Chapter 8: Microbial Metabolism 11/5Chapter 8: Microbial Metabolism Chapter 11: Microbial GeneticsQuiz 11: Chapter 8 (online)1111/10Chapter 11: Microbial Genetics 11/12Chapter 15: Microbial Mechanisms of PathogenicityQuiz 12: Chapter 11 (online)1211/17Ch.16: Disease & Epidemiology 11/19Chapter 17: Innate Nonspecific Immunity Quiz 13: Chapter 16 (online)1311/24Chapter 18: Specific Host Defenses EXAM 3: Ch 6, 8, 11, 15, & 1611/26THANKSGIVING HOLIDAY1412/1Chapter 18: Adaptive Specific Host Defenses Quiz 14: Chapter 17 (online)12/3Chapter 19: Diseases of the Immune System Quiz 15: Chapter 18 (online)*Disease Brochure Due1512/8 Chapter 21-26: Human Diseases12/10Chapter 21-26: Human DiseasesFinal Review-Last Class Quiz 16: Chapter 19 (online)1612/1512/17LECTURE FINAL EXAM5:30-7:30 PMTENTATIVE LABORATORY SCHEDULE-FALL 2020Dates are subject to change as neededWeekTuesdayLab Topic (Exercise #)Thursday1Sep 1Lab Orientation & Safety Video *Metric System, Introduction to Labster Simulations Labster 1: Biosafety SimulationSep 32Sep 8*Aseptic Technique (1-2); Quadrant Streak (1-3); *Microbes in the Environment (2-1)Labster 2: Atomic StructureSep 103Sep 15*Nutrient Broth & Nutrient Agar Preparation (p. 405)*Colony Morphology (2-2), *Growth on Slants (2-3), *in Broth (2-4); Preparation of Smears and Simple Staining (3-3)Labster 3: Food MacromoleculesSep 174Sep 22*Use and Care of the Microscope (3-1); Gram Stain (3-5) and Acid Fast (3-6)*Motility, Wet Mount & Hanging Drop, *Motility Agar (5-16)Labster 4: Gram StainSep 245Sep 29Capsule (3-7), & Endospore Stains (3-8)Morphological UnknownLabster 5: Identification of UnknownOct 16Oct 6LAB QUIZ #1Fungi, Protozoa & Helminths (3-2)Effect of Temperature on Bacterial Growth (2-8)Labster 6: Control of Microbial GrowthOct 87Oct 13Standard Plate Counts (6-1)Control of Bacterial Growth – UV light (2-10)Antimicrobial Drug Testing – Kirby-Bauer Method (7-2)Labster 7: Protein DenaturationOct 158Oct 20Control of Bacterial Growth – UV light (2-10)Chemical Disinfectants (handout)Labster 8: Cellular Respiration**Morphological Unknown report due**Oct 229Oct 27Biochemical UnknownOxidation-Fermentation (5-1), Phenol Red (5-2), MR-VP (5-3) testsLabster 9: FermentationOct 2910Nov 3Mannitol Salt Agar (4-1), Catalase (5-4), Oxidase (5-5), Starch (5-9), Blood Agar (5-14) testsReview for Lab Quiz 2Nov 511Nov 10LAB QUIZ #2Nitrate Reduction (5-6), Citrate (5-7), Decarboxylase (5-8), Urease (5-10) & SIM (5-18) testsLabster 10: Polymerase Chain ReactionNov 1212Nov 17Enterotube II (9-5)Water Testing-PR Lactose (Handout)PCR amplification of DNA (handout)Labster 11: Introduction to ImmunologyNov 1913Nov 24Water Testing-EMB agar (4-5)Labster 12: AntibodiesTHANKSGIVING-Non-Instruction Day – NO CLASS Nov 2614Dec 1LAB QUIZ #3Review for Laboratory Exam**Biochemical Unknown report due**Dec 3Thursday Group only:Water Testing-EMB agar (4-5)Labster 12: Antibodies15Dec 8Laboratory FINAL ExamDec 10* These exercises do not need to be entered into your lab notebook.Code of Academic Honor and IntegrityLAMC Department of Life SciencesBy enrolling in this course, you commit to integrity in all aspects of your work, whether quiz, exam, lab reports, or other assignment.? Unless your instructor explicitly states otherwise, the work you submit should be your own and convey what you yourself have actually learned.? This also means that you agree to follow the Standards of Student Conduct from the college catalog (pages 52-54) and will not engage in: copying another’s work; allowing someone else’s work to be submitted as if it was your own; using resources the instructor does not allow during assessments; submitting material without properly citing sources; or self-plagiarism, including submitting work completed for a previous attempt or another course.? Evidence of breaking this commitment will result in serious consequences and formal documentation of the incident. If you are unsure if an act constitutes plagiarism or any other violation of academic integrity, be sure to ask your instructor or librarian for clarification. ?MICROBIOLOGY 20 Course Objectives Upon completion of this course a successful student will:Employ microbiology terminology correctly in the laboratory and workplace.Analyze the differences between prokaryotic and eukaryotic cells. List subcellular structures and organelles of prokaryotic and eukaryotic cells and their functions, draw the structure of prokaryotic and eukaryotic cells and label their pare and contrast eukaryotic organisms including fungi, algae, and multicellular parasites using the compound light microscope, and describe important features.Describe the underlying mechanisms of chemical reactions in microbes, including energy and material pare and contrast the effect of pH, temperature, radiation, O2 concentration, osmotic pressure, and nutrients on microbial growth. Differentiate important events of the four growth stages of bacterial populations (lag, log, stationary, and decline). Describe the processes of DNA replication, mutations, transcription, and translation. Distinguish among mechanisms of genetic recombination in bacteria (transformation, transduction, and conjugation). Organize and name the major taxonomic categories of bacteria, fungi, viruses, algae, protozoa, and helminths.Demonstrate knowledge of the different features of prokaryotes, their different taxonomical groupings and to use reference sources for microbial identifications.Analyze the traits of important microbiological eukaryotes and their clinical and environmental relevance.Explain viral structure and the major steps in the life cycle of an animal virus. Describe the structure of a bacteriophage and distinguish between lytic and lysogenic life cycles. Examine and distinguish the stages of infectious disease (incubation, prodromal, illness, decline, and convalescence). Explain Koch’s postulates for infectious diseases and exceptions to them. Distinguish between bacterial endotoxins and exotoxins and give examples of each. Describe microbial mechanisms to enter host, evade defenses, and cause cellular damage.Explain the role of the nonspecific host defenses against microbial infection.Examine the features of IgA, IgD, IgM, IgG, and IgE immunoglobulins. Analyze specific host defenses against microbial infection, and distinguish between humoral and cell-mediated immunity. Discuss cells and cell fragments found in human blood and describe their functions in nonspecific and specific host responses.Distinguish among the major categories of immune disorders in humans, their signs and symptoms and the factors that may play a role in the etiology of such pare and contrast the major categories of antimicrobial drugs.Summarize the causes and characteristics of major human microbial diseases.NOTES & OTHER INFORMATION ................
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