Standard Preparations - University of Missouri

Autoclave 90-ml portions in 125-ml polypropylene bottles When cooled, to each bottle add 10 ml of separately autoclaved potassium phosphate buffer (0.17 M KH2PO4, 0.72 M K2HPO4). This buffer is made by dissolving 2.31 g KH2PO4 (anhydrous) and 12.54 g K2HPO4 (anhydrous) in 90 ml water, adjusting volume to 100 ml, and autoclaving. ................
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