Steps for primer design:

Max Primer Pair Tm Mismatch :1°C %GC Content : 20-80%. Number to return : 5 (can be changed as needed) Copy/Paste Primer data into the Word gene file and to the “d.htm” file. Copy/paste the candidate primer sequences to the bottom of the Word gene file and BLAST search each against the specific genome using “exact nucleotide match”. ................
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