Probablility that X2 will exceed tabulated values
Figure 15. Probe design settings in the Primer3 probe design tool. Click the “Pick Primers” button. You will now get a probe design to optimally hybridize with the sickle cell gene in a human blood sample (see Figure 16). Record your probe sequence for Question 8. Figure 16. Probe sequence obtained from Primer3 probe design tool. ................
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