Names: Key Hour: Date: /25 Points

Names: Key

Hour:

Date:

/25 Points

As a group of four, decide who is going to carry out which job (Jobs can be found on page 2). Follow the instructions below for the three numbers assigned to your group from the sentence code sheet (Page 3). Be sure to fill out the blanks on both pages 1 and 2 as you go.

1. The DNA stays in the nucleus (which is your assigned lab table). The DNA has the sentence code sheet out focusing on the # assigned to your lab group. This person's job is to actually read out loud the letter code from the code sheet. Remember: each sentence code represents one side of a DNA molecule. (3 Points)

#

#

#

2. The m-RNA transcribes each codon from the DNA, using base pairing rules, onto the lines below. This process is transcription; which is the first step of protein synthesis. (remember to use U instead of T) This step occurs in the nucleus. Example: DNA says "CCC" you write "GGG". (3 Points)

#

code:

#

code:

#

code:

3. The m-RNA leaves the nucleus with the transcribed code and goes to the cytoplasm (the rest of the classroom) to find the ribosome (the student desks). (1 point)

4. The m-RNA and the ribosome tell the t-RNA which anti-codons are needed (cards around

the room). The ribosome writes the anti-codon instructions on the lines below for t-RNA to

take when searching for the appropriate anti-codon and the corresponding amino acid. (3

Points)

#

anti-codon:

#

anti-codon:

#

anti-codon:

5. The t-RNA searches the cell (classroom) for the correct amino acid (word on the back of the anti-codon card) and begins to create the protein (sentence). This completes the translation step of protein synthesis. The t-RNA should write the sentence on the lines below. (3 Points)

#

protein:

#

protein:

#

protein:

LZHS ? Chapter 12 Protein Synthesis Lab

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Biology I CP

You are responsible for taking notes on each role. You must include information such as where each person performed their function, what their function was, which step of protein synthesis they were involved in, which types of nucleotides were involved, how many strands were involved, etc...

Person #1 = DNA (Name:

)

Where function performed (cytoplasm or nucleus) nucleus

What is their function stores information (read code)

Which step of protein synthesis was involved (transcription or translation)

Which nucleotides were involved (ATCG or AUCG) ATCG

How many strands were involved (one or two)

2

Other Important Info.

transcription

Person #2 = m-RNA (

Name:

)

Where function performed nucleus & cytoplasm

What is their function transcribes each codon from DNA

Which step of protein synthesis was involved

transcription

Which nucleotides were involved AUCG

How many strands were involved 1

Other Important Info.

Person #3 = r-RNA (Name:

)

Where function performed ribosome

What is their function

tell tRNA which anticodons are needed

Which step of protein synthesis was involved

transcription

Which nucleotides were involved AUCG

How many strands were involved 1

Other Important Info.

Person #4 = t-RNA (Name:

)

Where function performed cytoplasm

What is their function

find correct amino acid & begins to create protein

Which step of protein synthesis was involved

translation

Which nucleotides were involved AUCG

How many strands were involved 1

Other Important Info.

LZHS ? Chapter 12 Protein Synthesis Lab

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Biology I CP

Sentence Code Sheet

Each "sentence code" below represents one side of a DNA strand. You must imagine that you have "unzipped" a DNA strand and are now focusing on only one side. You will be transcribing the code into an m-RNA strand and further translating the code using a tRNA molecule with an appropriate amino acid attached. You will, however, be creating a sentence using words, rather than a protein using amino acids. Good Luck!

1. ATGAAAAACAAGGTACACATCTAG 2. ATGAAAAACAATTGCACGTAG 3. ATGTAAACCACTACATAG 4. ATGAGAAGTAGGAGAAGCATAATCTAG 5. ATGATTCAACACATCCAGCCACATTAG 6. ATGCCCCCGAGAAGCCCTTAG 7. ATGCGACGCCGGCGTTAG 8. ATGCTACTCATAGATCTGCTTTAG 9. ATGTAAAGGGAAGACGAGTAG 10. ATGCCCCCGGCAGCCGCGTAG 11. ATGGCTCCGAGAGGAGGCAGAGGGTAG 12. ATGAAAGGTAAGGTAGTCTAG 13. ATGAAAGTGAAGGTTTAG 14. ATGTAAAGGGAATACTATTCATAG 15. ATGTAATCCTCGTCTCGGCGTTAG 16. ATGATAGATCTGCTTCCGAGAAGCTAG 17. ATGCCCCCGGAATGATGCTAG 18. ATGTGGGTATGTCGGCGTTAG 19. ATGTTACCGAGATTCTTGTTTTAG

20. ATGTTATCCTCGTGGTTGTTTTAG

LZHS ? Chapter 12 Protein Synthesis Lab

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Biology I CP

AAC UAA CGA GUA UAU CGG GUG GAC AAU UCG CCG UGC UGG

MOTHER WE

DRINK A

THIS EVERY BROTHER DEMENTED DRESSES

BE IS YOU READ

LZHS ? Chapter 12 Protein Synthesis Lab

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Biology I CP

CGU UCU UUG GUC GAA UUA AGU CGC AUU CAA CUG GCU AGA

DAY INFORMED

FOR DRESS

ALL DNA BEATLES WATER AN OLD ROLL EDUCATION THE

LZHS ? Chapter 12 Protein Synthesis Lab

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Biology I CP

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